Abstract
MicroRNAs (miRNAs) regulate gene expression post-transcriptionally by binding the 3′ untranslated regions of target mRNAs. We examined the subcellular distribution of three miRNAs in exponentially growing HeLa cells and found that the vast majority are associated with mRNAs in polysomes. Several lines of evidence indicate that most of these mRNAs, including a known miRNA-regulated target (KRAS mRNA), are actively being translated.
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Acknowledgements
We thank the anonymous reviewers for helpful comments. This work was supported by grants from the US National Institutes of Health to T.W.N.
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Maroney, P., Yu, Y., Fisher, J. et al. Evidence that microRNAs are associated with translating messenger RNAs in human cells. Nat Struct Mol Biol 13, 1102–1107 (2006). https://doi.org/10.1038/nsmb1174
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DOI: https://doi.org/10.1038/nsmb1174
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