Abstract
We have isolated a large number of polynucleotide kinase ribozymes from a pool of RNA molecules consisting of an ATP-binding domain flanked by regions of random sequence. Different classes of kinases catalyse the transfer of the γ-thiophosphate of ATP-γS to the 5′-hydroxyl or to internal 2′-hydroxyls. An engineered version of one class is able to catalyse the transfer of thiophosphate from ATP-γS to the 5′-hydroxyl of an exogenous oligoribonucleotide substrate with multiple turnover, thus acting as a true enzyme.
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References
Ellington, A. D. & Szostak, J. W. Nature 346, 818–822 (1990).
Ellington, A. D. & Szostak, J. W. Nature 355, 850–852 (1992).
Famulok, M. & Szostak, J. W. J. Am. chem. Soc. 114, 3990–3991 (1992).
Sassanfar, M. & Szostak, J. W. Nature 364, 550–552 (1993).
Connel, G. J., Illangeskare, M. & Yarus, M. Biochemistry 32, 5497–5502 (1993).
Lorsch, J. R. & Szostak, J. W. Biochemistry 33, 973–982 (1994).
Famulok, M. J. Am. chem. Soc. 116, 1698–1706 (1994).
Jenison, R. D., Gill, S. C., Pardi, A. & Polisky, B. Science 263, 1425–1429 (1994).
Bartel, D., Zapp, M. L., Green, M. R. & Szostak, J. W. Cell 67, 529–536 (1991).
Tuerk, C. & Gold, L. Science 249, 505–510 (1990).
Bock, L. C., Griffin, L. C., Latham, J. A., Vermaas, E. H. & Toole, J. J. Nature 355, 564–566 (1992).
Tsai, D. E., Kenan, D. J. & Keene, J. D. Proc. natn. Acad. Sci. U.S.A. 89, 8864–8868 (1992).
Beaudry, A. A. & Joyce, G. F. Science 257, 635–641 (1992).
Green, R. & Szostak, J. W. Science 258, 1910–1915 (1992).
Lehman, N. & Joyce, G. F. Nature 361, 182–185 (1993).
Pan, T. & Uhlenbeck, O. C. Biochemistry 31, 3887–3895 (1992).
Bartel, D. P. & Szostak, J. W. Science 261, 1411–1418 (1993).
Prudent, J. R., Uno, T. & Schultz, P. G. Science 264, 1924–1927 (1994).
Richardson, C. C. in The Enzymes Vol. 14 (ed. Boyer, P.) 299–314 (Academic, New York, 1981).
Zimmerman, S. B. & Pheiffer, B. H. in The Enzymes Vol. 14 (ed. Boyer, P.) 315–329 (Academic, New York, 1981).
Zaug, A. J. & Cech, T. R. Biochemistry 25, 4478–4482 (1986).
Cadwell, R. C. & Joyce, G. F. PCR Meth. Appl. 2, 28–33 (1992).
Milligan, J. F. & Uhlenbeck, O. C. Meth. Enzym. 180, 51–64 (1989).
Westaway, S. K., Belford, H. G., Apostol, B. L., Abelson, J. & Greer, C. L. J. biol. Chem. 268, 2435–2443 (1993).
Domdey, H. & Gross, H. J. Analyt. Biochem. 98, 346–352 (1979).
Konarska, M., Filipowicz, W., Domdey, H. & Gross, H. J. Nature 293, 112–116 (1981).
Guerrier-Takada, C., Gardiner, K., Marsh, T., Pace, N. & Altman, S. Cell 35, 849–857 (1983).
Bass, B. & Cech, T. R. Nature 308, 820–826 (1984).
Chowrira, B. M., Berzal-Herranz, A. & Burke, J. M. Nature 354, 320–322 (1991).
Perrotta, A. T. & Been, M. D. Biochemistry 31, 16–21 (1992).
Dahm, S. C., Derrick, W. B. & Uhlenbeck, O. C. Biochemistry 32, 13040–13045 (1993).
Pyle, A. M. & Green, J. B. Biochemistry 33, 2716–2725 (1994).
Lillehaug, J. R. & Kleppe, K. Biochemistry 14, 1221–1225 (1975).
Herschlag, D., Piccirilli, J. A. & Cech, T. R. Biochemistry 30, 4844–4854 (1991).
Breslow, R. & Katz, I. J. Am. chem. Soc. 90, 7376–7377 (1968).
Ramirez, F., Marecek, J. F. & Szamosi, J. J. Org. Chem. 45, 4748–4752 (1980).
Gilbert, W. Nature 319, 618 (1986).
Eckstein, F. A. Rev. Biochem. 54, 367–402 (1985).
Fersht, A. Enzyme Structure and Mechanism (Freeman, New York, 1985).
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Lorsch, J., Szostak, J. In vitro evolution of new ribozymes with polynucleotide kinase activity. Nature 371, 31–36 (1994). https://doi.org/10.1038/371031a0
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DOI: https://doi.org/10.1038/371031a0
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