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Crystal structure of a yeast TBP/TATA-box complex

Nature volume 365, pages 512–520 (1993)Cite this article

Abstract

The 2.5 Å crystal structure of a TATA-box complex with yeast TBP shows that the eight base pairs of the TATA box bind to the concave surface of TBP by bending towards the major groove with unprecedented severity. This produces a wide open, underwound, shallow minor groove which forms a primarily hydrophobic interface with the entire under-surface of the TBP saddle. The severe bend and a positive writhe radically alter the trajectory of the flanking B-form DNA.

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Authors and Affiliations

  1. Department of Molecular Biophysics and Biochemistry and the Howard Hughes Medical Institute, Yale University, New Haven, Connecticut, 06510, USA

    Youngchang Kim, James. H. Geiger & Paul B. Sigler

  2. Fred Hutchinson Cancer Research Center, Seattle, Washington, 98104, USA

    Steven Hahn

Authors
  1. Youngchang Kim
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  2. James. H. Geiger
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  3. Steven Hahn
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  4. Paul B. Sigler
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Kim, Y., Geiger, J., Hahn, S. et al. Crystal structure of a yeast TBP/TATA-box complex. Nature 365, 512–520 (1993). https://doi.org/10.1038/365512a0

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