Abstract
The growth-promoting and/or differentiation-blocking activities of Kirsten (Ki-MSV) or Harvey murine sarcoma virus (Ha7–MSV) on various types of cells in vitro are well documented1–8. Here we report an unexpected effect of these viruses on a rat phaeochromocytoma cell line, PC12. PC12 cells, which multiply indefinitely in growth medium, are known to respond to nerve growth factor (NGF) by cessation of cell division and expression of several properties resembling those of differentiated sympathetic neurones9,10. We have found that Ki- and Ha-MSV mimic some, if not all, of the activities of NGF in PC12 cells, and there is evidence that the viral oncogenes, v-Ki-ras and v-Ha-ras, are responsible for this phenomenon. This system may be of value for studying the mechanism of action of the v-ras genes as well as the regulatory mechanism of growth and differentiation in neuronal cells.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
- Purchase on SpringerLink
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Scher, C. D., Scolnick, E. M. & Siegler, R. Nature 256, 225–226 (1975).
Yoakum, G. H. et al. Science 227, 1174–1179 (1985).
Rhim, J. S. et al. Science 227, 1250–1252 (1985).
Colletta, G. et al. Molec. cell. Biol. 3, 2099–2109 (1983).
Pierce, J. H. & Aaronson, S. A. J. exp. Med. 156, 873–887 (1982).
Yuspa, S. H., Kilkenny, A. E., Stanley, J. & Lichti, U. Nature 314, 459–462 (1985).
Weissman, B. E. & Aaronson, S. A. Cell 32, 599–606 (1983).
Hankins, W. D. & Scolnick, E. M. Cell 26, 91–97 (1981).
Greene, L. A. & Tischler, A. S. Proc. natn. Acad. Sci. U.S.A. 73, 2424–2428 (1976).
Burstein, D. E. & Greene, L. A. in Molecular Approaches to Neurobiology (ed. Brown, I. R.) 159–177 (Academic, Toronto, 1982).
Ellis, R. W., Lowy, D. R. & Scolnick, E. M. Adv. viral Oncol. 1, 107–126 (1982).
Scolnick, E. M., Stephenson, J. R. & Aaronson, S. A. J. Virol. 10, 653–657 (1972).
Devouge, M. W., Mukherjee, B. B. & Pena, S. D. J. Virology 121, 327–344 (1982).
Rieger, F., Shelanski, M. L. & Greene, L. A. Devl Biol. 76, 238–243 (1980).
Dichter, M. A., Tischler, A. S. & Greene, L. A. Nature 268, 501–504 (1977).
Alemà, S., Casalbore, P., Agostini, E. & Tatò, F. Nature 316, 557–559 (1985).
Noda, M., Selinger, Z., Scolnick, E. M. & Bassin, R. H. Proc. natn. Acad. Sci. U.S.A. 80, 5602–5606 (1983).
Bradshaw, R. A. A. Rev. Biochem. 47, 191–216 (1978).
Yankner, B. A. & Shooter, E. M. A. Rev. Biochem. 51, 845–868 (1982).
Schubert, D., LaCorbiere, M., Whitlock, C. & Stallcup, W. Nature 273, 718–723 (1978).
Skaper, S. D. & Varon, S. Devl Biol. 98, 257–264 (1983).
Traynor, A. E. Devl Brain Res. 14, 205–210 (1984).
Pfenninger, K. H. & Johnson, M. P. Proc. natn. Acad. Sci. U.S.A. 78, 7797–7800 (1981).
Toda, T. et al. Cell 40, 27–36 (1985).
Broek, D. et al. Cell 41, 763–769 (1985).
Ellman, G. L., Courtney, K. D., Andres, V. Jr & Featherstone, R. M. Biochem. Pharmac. 7, 88–95 (1961).
Ogura, A. & Takahashi, M. Brain Res. 301, 323–330 (1984).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Noda, M., Ko, M., Ogura, A. et al. Sarcoma viruses carrying ras oncogenes induce differentiation-associated properties in a neuronal cell line. Nature 318, 73–75 (1985). https://doi.org/10.1038/318073a0
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1038/318073a0