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Soluble Arg-Gly-Asp Peptides Reduce Collagen Accumulation in Cultured Rat Hepatic Stellate Cells

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Abstract

Hepatic stellate cells play a central role inthe pathogenesis of liver fibrosis, both via productionof extracellular matrix proteins and through secretionof matrix metalloproteinases. In this study, effects of soluble cell adhesion peptides oncollagen type I accumulation and on expression of matrixmetalloproteinases were analyzed. First, we revealed theexpression of α5-integrin on hepaticstellate cells by immunostaining. Treatment with 100μg/ml of soluble Arg-Gly-Asp (RGD) peptides was foundto reduce accumulation of type I collagen without anyeffects on its transcriptional level in rat hepatic stellate cells, whereas a control peptideGly-Arg-Gly-Glu-Ser (GRGES) had no such effect. SolubleRGD peptides also increased the secretion of collagenaseby stellate cells. These data suggested that reduced accumulation of type I collagen caused by theRGD peptide ligation to integrins on hepatic stellatecells was partly due to stimulated expression ofcollagenase by stellate cells.

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Authors
  1. Hiroaki Iwamoto
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  2. Hironori Sakai
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  3. Kazuhiro Kotoh
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  4. Makoto Nakamuta
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  5. Hajime Nawata
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Iwamoto, H., Sakai, H., Kotoh, K. et al. Soluble Arg-Gly-Asp Peptides Reduce Collagen Accumulation in Cultured Rat Hepatic Stellate Cells. Dig Dis Sci 44, 1038–1045 (1999). https://doi.org/10.1023/A:1026633302985

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