Abstract
The effects of six microelements (Cu2+, Mn2+, Fe2+, Mo2+, Zn2+, Co2+) on the production of betalains and the growth of suspension cultures of Beta vulgaris were studied. The increase of Co2+ from 1–5 μM resulted in a 60% increment on the production of betalains. A positive effect of this divalent ion was only accomplished when it was added at the beginning of the culture. This was related to a doubling in the specific betalains production rate compared to B5 control medium. No effects on cell growth and ratio of betacyanines to betaxanthines were observed. Mo2+, Fe2+ and Cu2+ presented a positive but less marked effect, while the increase of Mn2+ did not show effects on the production of betalains compared to B5 control medium.
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References
Böhm H & Rink E (1988) Betalains. In: Vasil IK (ed) Cell Culture and Somatic Cell Genetics of Plants, Vol 1 (pp. 449–463). Academic Press, London
De Backer-Royer C, Vannereau A, Duret S, Villavignes R & Cosson L (1990) Action de metaux lourds Cu et Cd sur des cellules de Catharanthus roseus cultivées in vitro: adaptation et production alcaloïdique. Col. Inst. Nat. Res. Alim. 51: 247–251
Delgado-Vargas F, Jiménez-Aparicio AR & Paredes-López O (2000) Natural pigments: carotenoids, anthocyanins and betalains-characteristics, biosynthesis, processing, and stability. CRC Crit. Rev. Food Sci. Nut. 40(3): 173–289
Endress R (1976) Betacyanin-akkumulation in kallus von Portulacca grandiflora var. JR unter dem einfluss vonphytohormonem und Cu2+-ionen auf unterschiedlichen grundmedien. Biochem. Physiol. Pflanz. 16: 87–98
Fujita Y, Hara Y, Suga C & Morimoto T (1981) Production of shikonin derivatives by cell suspension cultures of Lithospermum erythrorizon. II. A new medium for the production of shikonin derivatives. Plant Cell Rep. 1: 61–63
Francis FJ (2000) Anthocyanins and betalains: composition and applications. Cereal Food World 45(5): 208–13
Furze JM, Rhodes MJC, Parr AJ, Robins RJ, Whitehead IM & Threlfall DR (1991) Abiotic factors elicit sesquiterpenoid phytoalexin production but not alkaloid production in transformed roots of Datura stramonium. Plant Cell Rep. 10: 111–114
Gamborg OL, Miller RA & Ojima K (1968) Nutrient requirement of suspension cultures of soybean root cells. Exp. Cell Res. 50: 151–158
Hinz UG, Fivaz J, Girod PA & Zyrd JP (1997) The gene coding for the DOPA dioxygenase involved in betalain biosynthesis in Amanita muscaria and its regulation. Mol. Gen. Genet. 256: 1–6
Huang S, Chen S, Wu K & Taung W (1995) Strategy for inducing pertinent cell line and optimization of the medium for Stizolobium hassjoo producing L-DOPA. J. Ferment. Bioeng. 79(4): 342–347
Jimenez, A (1995) Producción de colorantes de interés alimentario por cultivo de células vegetales: las betalaínas del betabel (Beta vulgaris L.) como caso de estudio. PhD Thesis ENCB-IPN, Mexico
Jimenez-Aparicio A & Gutierrez-Lopez G (1999) Production of food related colorants by culture of plant cells. The case of betalains. In: Fereidon SP, Kolodziejczyk J, Whitaker JR, Lopez-Munguia A & Fuller G (eds) Chemicals via Higher Plant Bioengineering (pp 195–210). Kluwer Academic/Plenum, NY
King PJ & Street HE (1977) Growth patterns in cell cultures. In: Street HE (ed) Plant Tissue and Cell Culture (pp 307–387). Blackwell, Oxford
Leathers RR, Davin C & Zrÿd JP (1992) Betalain producing cell cultures of Beta vulgaris L. var. Bikores monogerm (red beet). In Vitro Cell. Dev. Biol. 28P: 39–45
Mizukami H, Konoshima M & Tabata M (1977) Effect of nutritional factors on shikonin derivative formation in Lithospermun callus cultures. Phytochemistry. 16: 1183–1186
Ohlsson AB & Berglund T (1989) Effects of high MnSO4 levels on cardenolide accumulation by Digitalis lanata tissue cultures in light and darkness. J. Plant Physiol. 135: 505–507
Obrenovic S (1990) Effect of Cu(II) D-penicillamine on phytochorome-mediated betacyanin formation in Amaranthus caudatus seedlings. Plant Physiol. Biochem. 28: 639–646
Rodriguez M, Jimenez A A, Davila OG & Sepulveda J G (1994) Effect of carbon source on cell growth and betalains production in cell suspension culture of Beta vulgaris. Biotechnol. Lett. 16: 853–858
Rodriguez-Monroy M & Galindo E (1999) Broth rheology and metabolite production of Beta vulgaris suspension culture: a comparative study between cultures grown in shake flasks and in a stirred tank. Enz. Microb. Technol. 24(10): 687–693
Sri Andrijany V, Indrayanto G & Adi Soehono L (1999) Simultaneous effect of calcium, magnesium, copper and cobalt ions on sapogenin steroids content in callus cultures of Agave amaniensis. Plant Cell Tiss. Org. Cul. 55: 103–108
Suvarnalatha G, Rajendran L, Ravishankar GA & Venkataraman LV (1994) Elicitation of anthocyanin production in cell cultures of carrot (Daucus carota L.) by using elicitors and abiotic stress. Biotechnol. Lett. 16: 1275–1280
Threlfall DR & Whitehead IM (1988) The use of metal ions to induce the formation of secondary products in plant tissue culture. In: Robins RJ & Rhodes MJ (eds) Manipulating Secondary Metabolism in Culture (pp 51–56). Cambridge University, Melbourne
Trejo-Tapia G, Jimenez-Aparicio A, Rodriguez M, Sepulveda G, Salcedo G, Martinez B, Gutierrez G & De JesÚs A (1999) Influence of medium constituents on growth and betalain production in cell suspension cultures of Beta vulgaris. Asia-Pacific J. Mol. Biol. Biotechnol. 7(2): 167–172
Verpoorte R, Van der Heijden R, Ten Hoopen HJG & Memelink J (1999) Metabolic engineering of plant secondary metabolites pathways for the production of fine chemicals. Biotechnol. Lett. 21: 467–479
Zar JH (1984) Biostatistical Analysis. Prentice-Hall, Englewood Cliffs, NJ
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Trejo-Tapia, G., Jimenez-Aparicio, A., Rodriguez-Monroy, M. et al. Influence of cobalt and other microelements on the production of betalains and the growth of suspension cultures of Beta vulgaris. Plant Cell, Tissue and Organ Culture 67, 19–23 (2001). https://doi.org/10.1023/A:1011684619614
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DOI: https://doi.org/10.1023/A:1011684619614