Abstract
High deposition levels of atmospheric ammonia in the Netherlands have led to the major replacement of dwarf shrubs by grasses and to elevated nitrification rates in acid heathland soils. In order to study the efficacy of a naturally established grass-heath of Deschampsia flexuosa at capturing NO- 3, an outdoor 'mesocosm' lysimeter experiment was set up with relatively large and undisturbed soil columns from two Deschampsia-dominated heathland sites. One of the sites (Ede) had a relatively high rate of nitrate production, whereas the other (Hoorneboeg [HB]) showed practically no nitrate formation. For part of the Ede columns, the fate of labeled nitrate, split-applied at two rates (30 or 150 kg ha-1 yr-1) during two seasons, was studied. D. flexuosa was highly effective in acquiring fertilizer nitrate, as demonstrated by (1) distinct rises in foliar NRA, especially at high N; (2) increased 15N enrichments in all plant components with N rate; (3) significant increases in organic-N and carboxylate concentration in several plant compartments; and (4) clear shifts in biomass allocation in favour of the aboveground tissues.
After 18 months at low N, an average 39 and 23% of the applied N was immobilized in the plant and soil compartments, respectively; at high N rate, corresponding recoveries were 33 and 20%. Total leaching of nitrate (beyond a depth of 35 cm) from the unfertilized Ede columns corresponded to an annual loss of 1.9 kmol N ha-1, whereas leaching was virtually zero from HB columns. Relatively high amounts of N leached from the fertilized columns with apparent fertilizer recovery in the leachate reaching an average 60% at high N. However,15 N analyses revealed only recoveries of 2.0% (low N) and 7.2% (high N) of the applied N in the leachate. From columns where the plant cover had been removed, apparent and real leaching losses reached values of >100 and 10% of the applied N, respectively. Hence, soil-derived N appeared by far the major source of leaching. Unplanted and unfertilized HB columns displayed high rates of nitrification and leached high amounts of nitrate, suggesting a plant-induced repression of the in situ nitrification at this site.
On average, planted columns had lost 37% (low N) and 40% (high N) of the applied N, whereas unplanted lysimeters had lost 89% (for both low and high N). The N not recovered was presumed lost by denitrification due to favourable conditions with respect to nitrate concentration, moisture, carbon supply, and temperature.
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References
Aerts R 1993 Biomass and nutrient dynamics of dominant plant species from heathlands. In Heathlands: Patterns and Processes in a Changing Environment. Eds. R Aerts and G W Heil. pp 51–84. Kluwer Academic Publishers, Dordrecht.
Barraclough D, Hyden M J and Davies G P 1983 Fate of fertilizer nitrogen applied to grassland. I. Field leaching results. J. Soil Sci. 34, 483–497.
Bijay-Singh, Ryden J C and Whitehead D C 1989 Denitrification potential and actual rates of denitrification in soils under longterm grassland and arable cropping. Soil Biol. Biochem. 21, 897–901.
Bristow AW, Ryden J C and Whitehead D C 1987 The fate at several time intervals of 15N-labelled ammonium nitrate applied to an established grass sward. J. Soil Sci. 38, 245–254.
Broadbent F E and Nakashima T 1971 Effect of added salts on nitrogen mineralization in three California soils. Soil Sci. Soc. Am. Proc. 35, 457–460.
Cameron K C and Haynes R J 1986 Retention and movement of nitrogen in soils. In Mineral Nitrogen in the Plant-Soil System. Ed. R J Haynes. pp 166–241. Academic Press, New York.
Clark F E 1977 Internal cycling of 15nitrogen in shortgrass prairie. Ecology 58, 1322–1333.
Colbourn P and Dowdell R J 1984 Denitrification in field soils. Plant Soil 76, 213–226.
De Boer W, Duyts H and Laanbroek H J 1988 Autotrophic nitrification in a fertilized acid heath soil. Soil Biol. Biochem. 20, 845–850.
De Boer W, Klein Gunnewiek P J A and Troelstra S R 1990 Nitrification in Dutch heathland soils. II. Characteristics of nitrate production. Plant Soil 127, 193–200.
Destain J P, Guiot J, Francois E and Riga A 1989 Fertilizer nitrogen budgets of two doses of Na15NO3 dressings split-applied to winter wheat in microplots on a loam soil. Plant Soil 117, 177– 183.
Dowdell R J, Webster C P, Hill D and Mercer E R 1984 A lysimeter study of the fate of fertilizer nitrogen in spring barley crops grown on shallow soil overlying Chalk: crop uptake and leaching losses. J. Soil Sci. 35, 169–181.
Firestone M K 1982 Biological denitrification. In Nitrogen in Agricultural Soils. Ed. F J Stevenson. Agronomy 22, 289–326. ASACSA-SSSA, Madison, WI.
Hart P B S, Rayner J H and Jenkinson D S 1986 Influence of pool substitution on the interpretation of fertilizer experiments with 15N. J. Soil Sci. 37, 389–403.
Hauck R D, Meisinger J J and Mulvaney R L 1994 Practical considerations in the use of nitrogen tracers in agricultural and environmental research. In Methods of Soil Analysis, Part 2. Microbiological and Biochemical Properties. Eds. R W Weaver et al. SSSA Book Series, no. 5, pp 907–950. SSSA, Madison, WI.
Haynes R J 1986 The decomposition process: mineralization, immobilization, humus formation, and degradation. In Mineral Nitrogen in the Plant-Soil System. Ed. R J Haynes. pp 52–126. Academic Press, New York.
Jansson S L 1971 Use of 15N in studies of soil nitrogen. In Soil Biochemistry, Vol. 2. Eds. A D McLaren and J Skujiņš. pp 129–166. Marcel Dekker, New York.
Jansson S L and Persson J 1982 Mineralization and immobilization of soil nitrogen. In Nitrogen in Agricultural Soils. Ed. F J Stevenson. Agronomy 22, 229–252. ASA-CSA-SSSA, Madison, WI.
Jenkinson D S, Fox R H and Rayner J H 1985 Interactions between fertilizer nitrogen and soil nitrogen – the so-called 'priming' effect. J. Soil Sci. 36, 425–444.
Jordan C 1989 The effect of fertiliser type and application rate on denitrification losses from cut grassland in Northern Ireland. Fertil. Res. 19, 45–55.
Kreutzer K, Göttlein A, Pröbstle P 1991 Dynamik und chemische Auswirkungen der Auflösung von Dolomitkalk unter Fichte (Picea abies [L.] Karst.). In Ö kosystemforschung Höglwald. Eds. K Kreutzer and A Göttlein. pp 186–204. Parey, Hamburg-Berlin, Germany.
Kundler P 1970 Ausnutzung, Festlegung und Verluste von D¨ungemittelstickstoff. Albrecht-Thaer-Archiv 14, 191–210.
Legg J O and Meisinger J J 1982 Soil nitrogen budgets. In Nitrogen in Agricultural Soils. Ed. F J Stevenson. Agronomy 22, 467–502. ASA-CSA-SSSA, Madison, WI.
Léon M, Lainé P, Ourry A and Boucaud J 1995 Increased uptake of native soil nitrogen by roots of Lolium multiflorum Lam. after nitrogen fertilization is explained by a stimulation of the uptake process itself. Plant Soil 173, 197–203.
MacDonald A J, Powlson D S, Poulton P R and Jenkinson DS 1989 Unused fertiliser nitrogen in arable soils – its contribution to nitrate leaching. J. Sci. Food Agric. 46, 407–419.
McCracken D V, Smith M S, Grove J H, MacKown C T and Blevins R L 1994 Nitrate leaching as influenced by cover cropping and nitrogen source. Soil Sci. Soc. Am. J. 58, 1476–1483.
Nielsen N E, Schjørring J K and Jensen H E 1988 Efficiency of fertilizer nitrogen uptake by spring barley. In Nitrogen Efficiency in Agricultural Soils. Eds. D S Jenkinson and K A Smith. pp 62–72. Elsevier Applied Science, London, New York.
Overrein L N 1967 Immobilization and mineralization of tracer nitrogen in forest raw humus. I. Effect of temperature on the interchange of nitrogen after addition of urea-, ammonium-and nitrate-N15. Plant Soil 27, 1–19.
Power J F and Legg J O 1984 Nitrogen-15 recovery for five years after application of ammonium nitrate to crested wheatgrass. Soil Sci. Soc. Am. J. 48, 322–326.
Rao A C S, Smith J L, Papendick R I and Parr J F 1991 Influence of added nitrogen interactions in estimating recovery efficiency of labeled nitrogen. Soil Sci. Soc. Am. J. 55, 1616–1621.
Reddy G B and Reddy K R 1993 Fate of nitrogen-15 enriched ammonium nitrate applied to corn. Soil Sci. Soc. Am. J. 57, 111–115.
Ryden J C 1983 Denitrification loss from a grassland soil in the field receiving different rates of nitrogen as ammonium nitrate. J. Soil Sci. 34, 355–365.
Sapozhnikov N A, Nesterova E I, Rusinova I P, Sirota L B and Livanova T K 1968 The effect of fertilizer nitrogen on plant uptake of nitrogen from different podzolic soils. Trans. 9th Int. Congr. Soil Sci. II, 467–474.
Schneider U and Haider K 1992 Denitrification-and nitrate leachinglosses in an intensively cropped watershed. Z. Pflanzenernähr. Bodenk. 155, 135–141.
Sexstone A J, Parkin T B and Tiedje J M 1985 Temporal response of soil denitrification rates to rainfall and irrigation. Soil Sci. Soc. Am. J. 49, 99–103.
Stark J M, Hart S C 1997 High rates of nitrification and nitrate turnover in undisturbed coniferous forests. Nature 385, 61–64.
Troelstra S R, Wagenaar R and De Boer W 1990 Nitrification in Dutch heathland soils. I. General soil characteristics and nitrification in undisturbed soil cores. Plant Soil 127, 179–192.
Troelstra S R, Wagenaar R and Smant W 1995a Nitrogen utilization by plant species from acid heathland soils. I. Comparison between nitrate and ammonium nutrition at constant low pH. J. Exp. Bot. 46, 1103–1112.
Troelstra S R, Wagenaar R and Smant W 1995b Nitrogen utilization by plant species from acid heathland soils. II. Growth and shoot/root partitioning of NO -3 assimilation at constant low pH and varying NO -3 /NH -4 ratio. J. Exp. Bot. 46, 1113–1121.
Troelstra S R, Wagenaar R, Smant W and De Boer W 1995c Soil nitrogen transformations and nitrate utilization by Deschampsia flexuosa (L.) Trin. at two contrasting heathland sites. Plant Soil 176, 81–93.
Van Vuuren M M I, Aerts R, Berendse F and De Visser W 1992 Nitrogen mineralization in heathland ecosystems dominated by different plant species. Biogeochemistry 16, 151–166.
Vermoesen A, De Groot C-J, Nollet L, Boeckx P and Van Cleemput O 1996 Effect of ammonium and nitrate application on the NO and N2O emission out of different soils. Plant Soil 181, 153– 162.
Walters D T and Malzer G L 1990a Nitrogen management and nitrification inhibitor effects on nitrogen-15 urea: I. Yield and fertilizer use efficiency. Soil Sci. Soc. Am. J. 54, 115–122.
Walters D T and Malzer G L 1990b Nitrogen management and nitrification inhibitor effects on nitrogen-15 urea: II. Nitrogen leaching and balance. Soil Sci. Soc. Am. J. 54, 122–130.
Westerman R L and Tucker T C 1974 Effects of salts and salts plus nitrogen-15-labeled ammonium chloride on mineralization of soil nitrogen, nitrification, and immobilization. Soil Sci. Soc. Am. Proc. 38, 602–605.
Whitehead D C and Dawson K P 1984 Nitrogen, including 15N labelled fertilizer nitrogen, in components of a grass sward. J. Appl. Ecol. 21, 983–989.
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Troelstra, S., Wagenaar, R. & Smant, W. Utilization and leaching of nitrate from two Deschampsia-dominated heathland sites: a lysimeter study using intact soil columns* . Plant and Soil 197, 41–53 (1997). https://doi.org/10.1023/A:1004206332608
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DOI: https://doi.org/10.1023/A:1004206332608