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CCAT2 is a lung adenocarcinoma-specific long non-coding RNA and promotes invasion of non-small cell lung cancer

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Tumor Biology

Abstract

The prognosis of non-small cell lung cancer (NSCLC) is still poor, and it is necessary to identify effectively diagnostic and prognostic biomarkers for NSCLC. Recent evidence demonstrates that long non-coding RNA (lncRNA) is actively transcribed from human genome. Some lncRNAs show a time- or tissue-specific expression manner and play important roles in diverse biological processes. Additionally, various cancer-associated lncRNAs have been identified, such as metastasis-associated lung adenocarcinoma transcript 1 for lung cancer. Here, we characterized the expression profile of a novel lncRNA, colon cancer-associated transcript 2 (CCAT2), in lung cancer and found that CCAT2 was significantly over-expressed in NSCLC tissues compared with paired adjacent normal tissues, with an average up-regulation fold of 7.5. Intriguingly, over-expression of CCAT2 was significantly associated with lung adenocarcinoma (p = 0.033) but not squamous cell cancer. Silencing CCAT2 by siRNA led to inhibition of proliferation and invasion in NSCLC cell lines in vitro. Additionally, CCAT2 combined with CEA could predict lymph node metastasis. Our findings indicate that CCAT2 is a lung adenocarcinoma-specific lncRNA and promotes invasion of NSCLC and highlight its potential as a biomarker for lymph node metastasis.

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Conflicts of interests

None

Funding sources

This study is funded by the Natural Science Foundation of China (81372321, 81201830), Natural Science Foundation for High Education of Jiangsu Province (13KJB320010), Research and Innovation Program for Graduates of Jiangsu Province (CXLX13_571), Human Resource Summit Grant of Jiangsu Province (10-D-078), and Key Clinical Medicine Technology Foundation of Jiangsu Province (BL2012030).

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Authors and Affiliations

  1. Jiangsu Key Laboratory of Molecular and Translational Cancer Research, Nanjing Medical University Affiliated Cancer Hospital, Cancer Institute of Jiangsu Province, Nanjing, 210009, China

    Mantang Qiu, Youtao Xu, Xin Yang, Jie Wang, Jingwen Hu, Lin Xu & Rong Yin

  2. Department of Thoracic Surgery, Nanjing Medical University Affiliated Cancer Hospital, Baiziting 42 Cancer Institute of Jiangsu Province, Nanjing, 210009, China

    Mantang Qiu, Youtao Xu, Xin Yang, Jingwen Hu, Lin Xu & Rong Yin

  3. The Fourth Clinical College, Nanjing Medical University, Nanjing, 210029, China

    Mantang Qiu

  4. The First Clinical College, Nanjing Medical University, Nanjing, 210029, China

    Youtao Xu & Xin Yang

  5. Department of Scientific Research, Nanjing Medical University Affiliated Cancer Hospital, Cancer Institute of Jiangsu Province, Nanjing, 210009, China

    Jie Wang

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  1. Mantang Qiu
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  2. Youtao Xu
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  3. Xin Yang
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  4. Jie Wang
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  6. Lin Xu
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  7. Rong Yin
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Corresponding authors

Correspondence to Lin Xu or Rong Yin.

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Mantang Qiu and Youtao Xu are contributed equally to this work

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Qiu, M., Xu, Y., Yang, X. et al. CCAT2 is a lung adenocarcinoma-specific long non-coding RNA and promotes invasion of non-small cell lung cancer. Tumor Biol. 35, 5375–5380 (2014). https://doi.org/10.1007/s13277-014-1700-z

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  • DOI: https://doi.org/10.1007/s13277-014-1700-z

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