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Modulating the growth of cysteine-capped cadmium sulfide quantum dots with enzymatically produced hydrogen peroxide

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Abstract

Cysteine (CSH) readily stabilizes cadmium sulfide quantum dots (CdS QDs) that grow in aqueous buffered solutions. The oxidation of CSH by hydrogen peroxide (H2O2) at room temperature yields cystine (CSSC), which is less efficient in stabilizing CdS QDs compared to CSH. Herein, we demonstrate that such oxidation causes a decrease in the formation rate of CSH-capped CdS QDs from Cd2+ and S2− ions. For the first time, we combined the oxidation of CSH with the glucose oxidase (GO x )-assisted biocatalytic oxidation of D-glucose, which leads to a buildup of H2O2 in the reaction mixture. The enzymatically modulated in situ growth of CdS QDs was monitored using two techniques: fluorescence spectroscopy and photoelectrochemical (PEC) analysis. This system enables quantification of GO x and glucose in human serum.

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Acknowledgements

This work was supported by the Spanish Ministry of Economy and Competitiveness (grant number BIO2014-59741-R).

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Authors and Affiliations

  1. Biosensing Unit, CIC BiomaGUNE, Parque Tecnológico de San Sebastian, Paseo Miramón 182, Donostia-San Sebastián, 20014, Spain

    Ruta Grinyte, Javier Barroso, Laura Saa & Valeri Pavlov

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  1. Ruta Grinyte
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  2. Javier Barroso
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  3. Laura Saa
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  4. Valeri Pavlov
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Correspondence to Valeri Pavlov.

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Grinyte, R., Barroso, J., Saa, L. et al. Modulating the growth of cysteine-capped cadmium sulfide quantum dots with enzymatically produced hydrogen peroxide. Nano Res. 10, 1932–1941 (2017). https://doi.org/10.1007/s12274-016-1378-1

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  • DOI: https://doi.org/10.1007/s12274-016-1378-1

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