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Early development of human hematopoietic and acquired immune systems in new born NOD/Scid/Jak3null mice intrahepatic engrafted with cord blood-derived CD34+ cells

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Abstract

An animal model in which the human immune system can be reconstituted is necessary to study acquired immunity in vivo. We report here a novel model, the NOD/SCID/JAK3null mouse, for the human immune system’s development. Newborn mice transplanted with human cord blood CD34+ cells intrahepatically, developed human T and B cells, and myeloid and plasmacytoid dendritic cells. The T and B cells had a naïve to memory phenotype, and included plasma cells. The human acquired immune system can be reconstituted from CD34+ cells in NOD/SCID/JAK3null mice. This model is a powerful tool for the study of human immunity.

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Acknowledgments

We are grateful to Dr. K. Matsui and his coworkers (Fukuda Hospital, Kumamoto, Japan) for providing cord blood. We thank I. Suzu for technical assistance and Y. Endo for secretarial assistance. This work was supported in part by Health and Labour Sciences Research Grants from the Ministry of Health, Labour and Welfare of Japan (H-16-AIDS-003 and H19-AIDS-003), by grants from the Ministry of Education, Science, Sports, and Culture of Japan, and by a Grant from the Uehara Memorial Foundation.

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Authors and Affiliations

  1. Division of Hematopoiesis, Center for AIDS Research, Kumamoto University, 2-2-1, Honjo, Kumamoto, 860-0811, Japan

    Seiji Okada, Hideki Harada & Shinya Suzu

  2. Department of Pathology and Experimental Medicine, Graduate School of Medical Sciences, Kumamoto University, Kumamoto, Japan

    Takaaki Ito

  3. Laboratory for Cell Signaling, RIKEN Research Center for Allergy and Immunology, Yokohama, 230-0045, Japan

    Takashi Saito

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  1. Seiji Okada
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  2. Hideki Harada
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  3. Takaaki Ito
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  4. Takashi Saito
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  5. Shinya Suzu
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Correspondence to Seiji Okada.

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Okada, S., Harada, H., Ito, T. et al. Early development of human hematopoietic and acquired immune systems in new born NOD/Scid/Jak3null mice intrahepatic engrafted with cord blood-derived CD34+ cells. Int J Hematol 88, 476–482 (2008). https://doi.org/10.1007/s12185-008-0215-z

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  • DOI: https://doi.org/10.1007/s12185-008-0215-z

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