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Selenium Deficiency Induces Inflammation via the iNOS/NF-κB Pathway in the Brain of Pigs

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Abstract

Selenium (Se) is an essential trace element to maintain homeostasis in humans and animals. The aim of the present study was to clarify the mechanism of Se deficiency-induced inflammation in the pig’s brain. Twenty-four healthy pigs were randomly divided into two groups (n = 12/group): control group (group C) was fed diet with 0.3 mg/kg inorganic Se, and Se-deficient group (group L) was fed diet with 0.007 mg/kg inorganic Se. At the 90th day of the experiment, the histology in the pig’s brain was observed by the microscope, the NO levels and iNOS activity were assayed, and the mRNA and protein expression levels of inflammatory cytokines (iNOS, COX-2, NF-κB, and PTGEs) and HSPs (HSP27, HSP40, HSP60, HSP70, and HSP90) were detected by real-time quantitative PCR and Western blot. Compared with group C, both of NO levels and iNOS activity were increased in group L, and the mRNA and protein expression levels of inflammatory cytokines (iNOS, COX-2, NF-κB, and PTGEs) and HSPs (HSP27, HSP40, HSP60, HSP70, and HSP90) were also upregulated; histological observation displayed inflammatory response in the brain of pig. In summary, diet with Se deficiency can activate the iNOS/NF-κB pathway to upregulate the expression of inflammatory cytokines, thereby leading to inflammatory lesions in the pig’s brain, and HSPs are involved in the compensatory regulation of inflammation. This study provides a reference for the prevention of pig brain inflammation from the perspective of nutrition.

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Acknowledgments

The study was supported by the Earmarked Fund for China Agriculture Research System (no. CARS 35).

Conflict of Interest

The authors declare that they have no conflict of interest.

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  1. Yilei Zhang and Jiawen Cui contributed equally to this work.

Authors and Affiliations

  1. College of Veterinary Medicine, Northeast Agricultural University, Harbin, 150030, People’s Republic of China

    Yilei Zhang, Jiawen Cui, Yingfei Lu, Chunzheng Huang, Honggui Liu & Shiwen Xu

  2. Key Laboratory of the Provincial Education Department of Heilongjiang for Common Animal Disease Prevention and Treatment, College of Veterinary Medicine, Northeast Agricultural University, Harbin, 150030, People’s Republic of China

    Shiwen Xu

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Zhang, Y., Cui, J., Lu, Y. et al. Selenium Deficiency Induces Inflammation via the iNOS/NF-κB Pathway in the Brain of Pigs. Biol Trace Elem Res 196, 103–109 (2020). https://doi.org/10.1007/s12011-019-01908-y

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