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MFα Signal Peptide Enhances the Expression of Cellulase eg1 Gene in Yeast

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Abstract

Ethanol production from lignocellulose by recombinant yeast with high level expression of heterologous cellulase genes has been a major anticipation. The native secretion signal sequence of the cellulase endoglucanase I (eg1) gene was replaced by Saccharomyces cerevisiae mating factor α prepro-leader sequence (MFα). The transformants containing native secretion signal (Y 1) and MFα secretion signal (Y 2) were characterized with respect to gene expression and growth on cellulose substrate. Increased enzyme activity and cellulose utilization were observed. The enzyme activity of Y 2 was 0.084 U/ml, 61.5% higher than Y 1 (0.052 U/ml). The sufficiency parameter (S value) was raised from 0.6 to 0.84. MFα signal peptide was more efficient than the native signal peptide of eg1 gene, suggesting that signal peptide replacement is an efficient way to enhance the cellulase expression level in yeast, for cellulose-derived ethanol production.

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References

  1. Börjesson, J., Peterson, R., & Tjerneld, F. (2007). Enzyme and Microbial Technology, 40(4, 5), 754–762.

    Article  Google Scholar 

  2. Lynd, L. R., Van Zyl, W. H., Mcbride, J., & Laser, M. (2005). Current Opinion in Biotechnology, 16, 577–583.

    Article  CAS  Google Scholar 

  3. Zaldivar, J., Nielsen, J., & Olsson, L. (2001). Applied Microbiology and Biotechnology, 56(1–2), 17–34.

    Article  CAS  Google Scholar 

  4. Sticklen, M. B. (2007). Feedstock Crop Genetic Engineering for Alcohol Fuels. Crop Science, 47(6), 2238–2248.

    Article  CAS  Google Scholar 

  5. Wyman, C. E. (2003). Biotechnology Progress, 19, 254–262.

    Article  CAS  Google Scholar 

  6. Penttilä, M. E., André, L., Saloheimo, M., Lehtovaara, P., & Knowles, J. K. (1987). Yeast, 3(3), 175–185.

    Article  Google Scholar 

  7. van Rooyen, R., Hahn-Hägerdal, B., La Grange, D. C., & van Zyl, W. H. (2005). Journal of Biotechnology, 120, 284–295.

    Article  Google Scholar 

  8. Den Haan, R., Rose, S. H., Lynd, L. R., & van Zyl, W. H. (2007). Metabolic Engineering, 9(1), 87–94.

    Article  Google Scholar 

  9. Fujita, Y., Ito, J., Ueda, M., Fukuda, H., & Kondo, A. (2004). Applied and Environmental Microbiology, 70, 1207–1212.

    Article  CAS  Google Scholar 

  10. Den Haan, R., McBride, J. E., Grange, D. C. L., Lynd, L. R., & Van Zyl, W. H. (2007). Enzyme and Microbial Technology, 40(5), 1291–1299.

    Article  Google Scholar 

  11. Han, Y., Wilson, D. B., & Lei, X. G. (1999). Applied and Environmental Microbiology, 65(5), 1915–1918.

    CAS  Google Scholar 

  12. van den Berg, M. A., & Steensma, H. Y. (1997). Yeast, 13(6), 551–559.

    Article  Google Scholar 

  13. Sambrook, J., & Russell, D. W. (2001). Molecular Cloning: A Laboratory Manual (3rd ed.). Cold Spring Harbor: Cold Spring Harbor Laboratory Press.

    Google Scholar 

  14. McBride, J. E., Zietsman, J. J., Van Zyl, W. H., & Lynd, L. R. (2005). Enzyme and Microbial Technology, 37, 93–101.

    Article  CAS  Google Scholar 

Download references

Acknowledgements

This work was supported by the National Natural Science Foundation of China (No. 50673078), the “863” Project of the Ministry of Science and Technology (No. 2007AA022004), the Innovation Program of Shanghai Municipal Education Commission (No. 08ZZ21), and the Yong faculty grant of Anhui Agricultural university (No. yj2008-25).

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Authors and Affiliations

  1. Key Laboratory of Microbial Control, Anhui Agricultural University, Hefei, 230036, People’s Republic of China

    Hong Zhu

  2. School of Life Science and Technology, TongJi University, Shanghai, 200092, People’s Republic of China

    Hong Zhu, Side Yao & Shilong Wang

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  1. Hong Zhu
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  2. Side Yao
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  3. Shilong Wang
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Correspondence to Shilong Wang.

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Zhu, H., Yao, S. & Wang, S. MFα Signal Peptide Enhances the Expression of Cellulase eg1 Gene in Yeast. Appl Biochem Biotechnol 162, 617–624 (2010). https://doi.org/10.1007/s12010-009-8880-9

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  • DOI: https://doi.org/10.1007/s12010-009-8880-9

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