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Physiological properties and chlorophyll biosynthesis in a Pak-choi (Brassica rapa L. ssp. chinensis) yellow leaf mutant, pylm

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Abstract

The chlorophyll (Chl)-deficient mutant pylm is a double haploid (DH) line of Pak-choi (Brassica rapa L. ssp. chinensis) that was obtained from Pak-choi variety ‘Huaguan’ by isolated microspore culture. In this study, the aim was to examine the physiological properties and Chl biosynthesis in the pylm mutant. Compared to the control line ‘CK-51’, pylm is slender, with an earlier flowering phenotype and obviously elongated hypocotyls. Normally grown pylm seedlings had yellow leaves with reduced total Chl content but an increased Chl a/b ratio. Ultrastructural analysis showed that the pylm mutant has a loose grana lamellae structure and few thylakoid stacks, resulting in impaired chloroplast development. Measurement of photosynthetic gas exchange and Chl fluorescence parameters indicated that the pylm mutant has lower photosynthetic activity and photochemical conversion efficiency than ‘CK-51’ plants. We investigated the biochemical mechanism of Chl deficiency in pylm by examining Chl biosynthesis precursors in this mutant, showing that Chl biosynthesis is blocked in the mutant at the Chl a production step. In addition, the expression of a number of genes related with Chl biosynthesis is down-regulated in pylm. Therefore, these results provide a basis for understanding the physiological and biochemical causes of the mutant phenotype of pylm.

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Abbreviations

ALA:

5-Aminolevulinate

PBG:

Porphobilinogen

Urogen III:

Uroporphyrinogen III

Coprogen III:

Coproporphyrinogen III

Proto IX:

Protoporphyrin IX

Mg-Proto IX:

Mg-protoporphyrin IX

Pchlide:

Protochlorophyllide

Chlide:

Chlorophyllide

qRT-PCR:

Quantitative reverse-transcription PCR

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Acknowledgements

This research was funded by grants from the National Natural Science Foundation of China (31071792), and the Specialized Research Fund for the Doctoral Program of Higher Education in China (20092103110001).

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    1. Department of Horticulture, Shenyang Agricultural University, 120 Dongling Road, Shenhe District, Shenyang, 110866, People’s Republic of China

      Kun Zhang, Zhiyong Liu, Xiaofei Shan, Chengyu Li, Xiaoyan Tang, Mingyu Chi & Hui Feng

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    1. Kun Zhang
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    Correspondence to Hui Feng.

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    Communicated by M. Horbowicz.

    K. Zhang and Z. Liu contributed equally to this work and share the first authorship.

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    Zhang, K., Liu, Z., Shan, X. et al. Physiological properties and chlorophyll biosynthesis in a Pak-choi (Brassica rapa L. ssp. chinensis) yellow leaf mutant, pylm . Acta Physiol Plant 39, 22 (2017). https://doi.org/10.1007/s11738-016-2321-5

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