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Expression of prolyl 4-hydroxylase beta-polypeptide in non-small cell lung cancer treated with Chinese medicines

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Abstract

Objectives

To investigate the role of prolyl 4-hydroxylase beta polypeptide (P4HB) expressed in lung carcinoma and the intervention effect of Yiqi Chutan Formula (益气除痰方, YQCTF).

Methods

Lung carcinoma model was established by subcutaneously inoculating LEWIS lung carcinoma cells in C57BL/6J mice. The differential expression of P4HB protein between the YQCTF (3.0 g/kg, gavage, once daily, 21 days) group and the control group was acquired by a 2 fluorescence difference gel electrophoresis (2D-DIGE), verified by Western blotting and identified by matrix-assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF/TOF-MS). The expression of P4HB and P4HB mRNA in cultured A549 cells from cisplatin (DDP) 1.5 µg/mL group and 15% serum combined with DDP 1.5 µg/mL group were detected by cellular immunohistochemistry and reverse transcription-polymerase chain reaction, respectively.

Results

The proteomics research discovered that one-third of differential proteins including P4HB were decreased in the YQCTF group (P<0.01). Clinical pathology and tissue microarray studies showed that P4HB expression in lung cancer tissue was stronger than adjacent tissues and normal lung epithelial (P<0.01). In the YQCTF and DDP combined groups, the expression of P4HB and P4HB mRNA in A549 cell were decreased significantly (P<0.01).

Conclusion

YQCTF could inhibit the LEWIS lung carcinoma's growth, decrease the expression of P4HB in LEWIS lung carcinoma and A549 cells. YQCTF might take effect through regulating P4HB in endoplasmic reticulum to inhibit the incidence and growth process of lung carcinoma.

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Authors and Affiliations

  1. Department of Clinical Teaching and Research, College of Traditional Chinese Medicine, Chongqing Medical University, Chongqing, 401331, China

    Shu-mei Wang  (王淑美)

  2. Department of Oncology, the First Affiliated Hospital of Guangzhou Traditional Chinese Medicine University, Guangzhou, 510405, China

    Li-zhu Lin  (林丽珠), Dai-han Zhou  (周岱翰) & Jing-xu Zhou  (周京旭)

  3. Department of Oncology, the Affiliated Hospital of Chengdu Traditional Chinese Medicine University, Chengdu, 610075, China

    Shao-quan Xiong  (熊绍权)

Authors
  1. Shu-mei Wang  (王淑美)
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  2. Li-zhu Lin  (林丽珠)
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  3. Dai-han Zhou  (周岱翰)
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  4. Jing-xu Zhou  (周京旭)
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  5. Shao-quan Xiong  (熊绍权)
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Corresponding author

Correspondence to Li-zhu Lin  (林丽珠).

Additional information

Supported by the National Natural Science Fundation of China (No. 30772862); Guangdong Natural Science Fund (No. 2010-3); Project of Guangdong University Talent Fund (No. 2010-79)

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Wang, Sm., Lin, Lz., Zhou, Dh. et al. Expression of prolyl 4-hydroxylase beta-polypeptide in non-small cell lung cancer treated with Chinese medicines. Chin. J. Integr. Med. 21, 689–696 (2015). https://doi.org/10.1007/s11655-013-1535-2

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  • DOI: https://doi.org/10.1007/s11655-013-1535-2

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