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Relationship between salivary Chromogranin-A and stress induced by simulated monotonous driving

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Abstract

The purpose of the present study was to evaluate the use of salivary Chromogranin-A (CgA), which is already used in general as a mental stress marker, for studying the stressful situation created by simulated monotonous driving. After informed consent, 25 healthy male and female subjects were studied under constant environment-controlled conditions. We measured the following physiological variables: blood pressure (BP), cardiac output, total peripheral resistance (TPR), normalized pulse volume (NPV) as an index of alpha-adrenergic sympathetic activity to the finger arteriolar vessels, levels of cortisol and CgA during monotonous driving. The induced stress led to the expected decreases in NPV and increases in TPR and BP caused by peripherally related sympathetic acceleration. However, CgA levels were found to fall gradually in accordance with the gradual increase of subjective rating of stress (SRS) and significantly (p < 0.01) decreased over the period of the simulated monotonous driving. Our hypothesis for the gradual decrease of CgA levels during the simulated monotonous driving is as follows. CgA, catestatin and catecholamines are co-released into the extra-cellular environment. Peripheral sympathetic activity was accelerated by stress resulting from the simulated monotonous driving. Upon peripheral vessel constriction, an increase in TPR then increased BP which, in turn, activated catestatin. Consequently, secretion of CgA was blocked by the co-secreted catestatin from chromaffin granules. The results obtained strongly indicate that, although CgA has been reported as a possible marker of stress, CgA levels are not increased in the stressful situation of simulated monotonous driving.

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Abbreviations

CgA:

Chromogranin-A

BP:

Blood pressure

CO:

Cardiac output

TPR:

Total peripheral resistance

NPV:

Normalized pulse volume

SRS:

Subjective rating of stress

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Acknowledgments

The authors would like to give their special thanks to Prof. Ken-ichi Yamakoshi, Kanazawa University, for his valuable suggestions and comments, and Mr. Kenta Matsumura, Hokkaido University, for his technical assistance. We would also like to give our sincere thanks to Prof. Peter Rolfe, OBH Ltd., UK, for his helpful comments on this work and for his assistance in preparing the manuscript. Financial support for this work was partially provided by The Knowledge-based Cluster Creation Project (Ishikawa High-Tech Sensing Cluster) and the Ministry of Education, Science, Sports and Culture, Grant-in-Aid for Scientific Research (B), 17300149, 2007.

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Authors and Affiliations

  1. Graduate School of Natural Science and Technology, Kanazawa University, Kakuma, Kanazawa, Ishikawa, 920-1192, Japan

    Takehiro Yamakoshi & Sang-Bum Park

  2. Department of Chemistry, School of Advanced Science, Dankook University, San#29 Anseo-dong, Cheonan-si, Chungnam, 330-714, Korea

    Won-Cheoul Jang

  3. Department of Electronic Engineering, School of Engineering, Dankook University, San#29 Anseo-dong, Cheonan-si, Chungnam, 330-714, Korea

    Kyungho Kim

  4. Graduate School of Science and Technology, Chiba University, 1-33 Yayoi-cho Inage-ku, Chiba, Chiba, 263-8522, Japan

    Yasuhiro Yamakoshi

  5. The Department of Business Administration, Kinjo College, 1200 Kasama-machi, Hakusan, Ishikawa, 924-8511, Japan

    Hajime Hirose

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  1. Takehiro Yamakoshi
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Correspondence to Takehiro Yamakoshi.

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Yamakoshi, T., Park, SB., Jang, WC. et al. Relationship between salivary Chromogranin-A and stress induced by simulated monotonous driving. Med Biol Eng Comput 47, 449–456 (2009). https://doi.org/10.1007/s11517-009-0447-y

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