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Design of a Computer-Assisted System to Automatically Detect Cell Types Using ANA IIF Images for the Diagnosis of Autoimmune Diseases

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Abstract

Indirect immunofluorescence technique applied on HEp-2 cell substrates provides the major screening method to detect ANA patterns in the diagnosis of autoimmune diseases. Currently, the ANA patterns are mostly inspected by experienced physicians to identify abnormal cell patterns. The objective of this study is to design a computer-assisted system to automatically detect cell patterns of IIF images for the diagnosis of autoimmune diseases in the clinical setting. The system simulates the functions of modern flow cytometer and provides the diagnostic reports generated by the system to the technicians and physicians through the radar graphs, box-plots, and tables. The experimental results show that, among the IIF images collected from 17 patients, 6 were classified as coarse-speckled, 3 as diffused, 2 as discrete-speckled, 1 as fine-speckled, 2 as nucleolar, and 3 as peripheral patterns, which were consistent with the patterns determined by the physicians. In addition to recognition of cell patterns, the system also provides the function to automatically generate the report for each patient. The time needed for the whole procedure is less than 30 min, which is more efficient than the manual operation of the physician after inspecting the ANA IIF images. Besides, the system can be easily deployed on many desktop and laptop computers. In conclusion, the designed system, containing functions for automatic detection of ANA cell pattern and generation of diagnostic report, is effective and efficient to assist physicians to diagnose patients with autoimmune diseases. The limitations of the current developed system include (1) only a unique cell pattern was considered for the IIF images collected from a patient, and (2) the cells during the process of mitosis were not adopted for cell classification.

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Acknowledgments

This study was supported in part by Ministry of Science and Technology of Taiwan under grant nos. NSC100-2410-H-166-007-MY3 and MOST103-2622-H-166-001.

Conflict of interests

The authors declare that there is no conflict of interests regarding the publication of this paper.

Author information

Authors and Affiliations

  1. Department of Electrical Engineering, National Chung Hsing University, Taichung, 402, Taiwan

    Chung-Chuan Cheng & Jin-Shiuh Taur

  2. Department of Electrical Engineering and Energy Technology, Chung Chou University of Science and Technology, Changhua, 510, Taiwan

    Chun-Feng Lu

  3. Division of Allergy, Immunology and Rheumatology, Taichung Veterans General Hospital, Taichung, 40705, Taiwan

    Tsu-Yi Hsieh

  4. Departments of Management Information System, Central Taiwan University of Science and Technology, Taichung, 40601, Taiwan

    Yaw-Jen Lin

  5. Departments of Health Services Administration, China Medical University, Taichung, 40402, Taiwan

    Yung-Fu Chen

  6. Department of Dental Technology and Materials Science, Central Taiwan University of Science and Technology, Taichung, 40601, Taiwan, Republic of China

    Yung-Fu Chen

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  1. Chung-Chuan Cheng
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  2. Chun-Feng Lu
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Correspondence to Yung-Fu Chen.

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This article is part of the Topical Collection on Smart Living in Healthcare and Innovations.

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Cheng, CC., Lu, CF., Hsieh, TY. et al. Design of a Computer-Assisted System to Automatically Detect Cell Types Using ANA IIF Images for the Diagnosis of Autoimmune Diseases. J Med Syst 39, 117 (2015). https://doi.org/10.1007/s10916-015-0314-3

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