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Identification and quantification of microcystins from a Nostoc muscorum bloom occurring in Oukaïmeden River (High-Atlas mountains of Marrakech, Morocco)

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Abstract

Health risks generated by cyanobacterial toxins in drinking and recreational waters are clearly recognised. During the monitoring programme on the distribution of toxic freshwater cyanobacteria in various water bodies including reservoirs, ponds and rivers of Morocco, many toxigenic cyanobacteria bloom-forming species have been identified. Particular attention was given to the investigation of the toxicology of a benthic Nostoc species—Nostoc muscorum Ag. (cyanobacteria, Nostocales, Nostocaceae)—that was found dominant in Oukaïmeden river located at 2,600 m of altitude in High-Atlas mountains of Marrakech. The massive growth of the mat-forming N. muscorum occurred yearly during the period of March–October, when the water temperature was above 10°C. During 1997–1999, samples were collected from either floating or benthic mats. Hepatotoxicity associated to gastrointestinal (diarrhoea) intoxication symptoms was confirmed by intraperitoneal (i.p.) injection in mice of N. muscorum thallus extract. The survival time was estimated to be from 2–5 h, and the calculated i.p. LD50 in mice ranged from 15 to 125 mg kg−1 body weight. The application of the high performance liquid chromatography with photodiode array detection confirmed the occurrence of microcystin-LR (MC-LR) and three others microcystin variants from the methanolic Nostoc extract. The MC-LR represented a proportion of 39% of the total microcystin content however, the total concentration equivalents—eq—of MC-LR was estimated to be 139 μg MC-LR eq per gram dry weight. The existence of a benthic microcystin-producing N. muscorum strain under the particular environmental conditions of Oukaïmeden region may be a potential human health hazard and the ecological harmful effects of these cyanobacterial toxins need to be assessed. This paper constitutes the first report of the occurrence of a toxic benthic Nostoc in Morocco. So, the benthic species should be considered during monitoring of toxic Cyanobacteria particularly for river used for source of drinking water.

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Authors and Affiliations

  1. Faculty of Sciences Semlalia-Marrakech, Department of Biology, Laboratory of Biology and Biotechnology of Micro-organisms, Microbiology and Toxicology Environmental University, P.O. Box 2390, 40 000, Marrakech, Morocco

    B. Oudra

  2. Laboratoire de cytologie et d’Anatomopathologie, N°186 Avenue Mohammed V, Marrakech, Morocco

    M. Dadi-El Andaloussi

  3. Departamento de Zoologia e Antropologia, Faculdade de Ciências, Universidade do Porto, 4050, Porto, Portugal

    V. M. Vasconcelos

  4. CIIMAR/CIMAR, Centre of Marine and Environmental Research, R. Dos Bragas, 289-4050-123, Porto, Portugal

    V. M. Vasconcelos

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  1. B. Oudra
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  2. M. Dadi-El Andaloussi
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  3. V. M. Vasconcelos
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Correspondence to V. M. Vasconcelos.

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Oudra, B., Dadi-El Andaloussi, M. & Vasconcelos, V.M. Identification and quantification of microcystins from a Nostoc muscorum bloom occurring in Oukaïmeden River (High-Atlas mountains of Marrakech, Morocco). Environ Monit Assess 149, 437–444 (2009). https://doi.org/10.1007/s10661-008-0220-y

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  • DOI: https://doi.org/10.1007/s10661-008-0220-y

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