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Evaluation of oyster mushroom strains for resistance to Pseudomonas tolaasii by inoculation in spawned substrates

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Abstract

Bacterial brown blotch disease, caused by Pseudomonas tolaasii, has been one of the most serious bacterial diseases of the oyster mushroom. Resistance to disease has been a priority in breeding programs for the oyster mushroom. In this study, a set of 37 Pleurotus ostreatus strains was tested for resistance to the pathogen P. tolaasii by two methods, with inoculations on pileus and spawned substrate under controlled environmental conditions. The results showed that the protocol with inoculation on spawned substrate was sensitive and adequate for testing resistance of oyster mushroom to brown blotch disease. According to the disease severity of fruiting bodies with inoculation on spawned substrates, strain ACCC50618 was resistant; ACCC50236 was moderate resistant; and the others were susceptible to the pathogen P. tolaasii (ACCC 01267). However, it was difficult to differentiate the resistance of P. ostreatus strains to P. tolaasii by inoculation on the pileus. This is the first report about resistance evaluation of P. ostreatus to brown blotch disease.

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Acknowledgements

This work was supported by the National Natural Science Foundation of China (No. 31101588), and the China agriculture research system (No.CARS-24).

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Authors and Affiliations

  1. Key Laboratory of Microbial Resources Collection and Preservation, Ministry of Agriculture, and Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, Beijing, 100081, China

    Rui Ying Zhang, Jin Gang Gu, Xue Mei Zuo, Qing Xiu Hu & Jin Xia Zhang

  2. Beijing Academy of Science and Technology, Beijing, 100089, China

    Dan Dan Hu

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  1. Rui Ying Zhang
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  2. Dan Dan Hu
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  4. Xue Mei Zuo
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Correspondence to Qing Xiu Hu or Jin Xia Zhang.

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Zhang, R.Y., Hu, D.D., Gu, J.G. et al. Evaluation of oyster mushroom strains for resistance to Pseudomonas tolaasii by inoculation in spawned substrates. Eur J Plant Pathol 137, 119–126 (2013). https://doi.org/10.1007/s10658-013-0223-6

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