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Can cutin and suberin biomarkers be used to trace shoot and root-derived organic matter? A molecular and isotopic approach

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Abstract

Cutins of plant shoots and suberins, mostly present in roots could contribute to significant portions of stable soil organic matter. Their biomarker potential, residing in their unique compositions in different plant types, has been used previously to infer sources of organic matter in sediments. These aliphatic plant biopolyesters contain specific biomarkers, which may be used for tracing their fate in soils and sediments, when combined with stable 13C isotope labelling. In order to evaluate the potential use of cutin and suberin biomarkers as indicators of shoot and root contributions from C3 and C4 plant origins, the objectives of this study were to 1) identify their constitutive monomers, which are specific for shoots and roots of maize (C4) and wheat (C3); 2) evaluate the 13C differences between maize and wheat biomarkers. Mid-chain hydroxy carboxylic acids were mainly found in the aboveground biomass, while α,ω-alkanedioic acids were only present in the roots. The differences in the isotopic composition of the specific monomers between wheat and maize plants (17–18‰ for shoot markers, 19‰ for root markers) were larger than those observed for bulk plant tissues and close to those observed for lignin-derived phenols in other studies. These differences should make it possible to differentiate and quantify the different types and sources of organic matter in sediments and soils. In particular, the molecular and isotopic signatures of cutins and suberins can be used to evaluate the specific dynamics of root vs shoot tissues in soils using C3/C4 chronosequences.

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Acknowledgements

This research was made possible through a grant from the ‘Ministère délégué à la Recherche et aux Nouvelles Technologies’—ACI no. JC10052. The authors wish to thank Gérard Bardoux for technical support during chromatographic and isotopic analysis. We thank two anonymous reviewers and Jeremy Jacob for their constructive comments.

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  1. UMR Bioemco (Biogéochimie et Ecologie des Milieux continentaux), CNRS, UPMC, Bldg Eger, 78850, Thiverval-Grignon, France

    Mercedes Mendez-Millan, Marie-France Dignac & Cornelia Rumpel

  2. UMR Bioemco, CNRS 7618, UPMC, 4, place Jussieu, 75252, Paris Cedex 05, France

    Sylvie Derenne

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  1. Mercedes Mendez-Millan
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  2. Marie-France Dignac
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Correspondence to Marie-France Dignac.

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Mendez-Millan, M., Dignac, MF., Rumpel, C. et al. Can cutin and suberin biomarkers be used to trace shoot and root-derived organic matter? A molecular and isotopic approach. Biogeochemistry 106, 23–38 (2011). https://doi.org/10.1007/s10533-010-9407-8

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