Nothing Special   »   [go: up one dir, main page]
Skip to main content

Advertisement

Springer Nature Link
Log in

Allozyme Variation and Population Genetic Structure in the Carpet Shell Clam Ruditapes decussatus Across the Siculo-Tunisian Strait

  • Published:
Biochemical Genetics Aims and scope Submit manuscript

Abstract

This study reports on the polymorphism of 15 allozyme loci in Ruditapes decussatus clams collected from 11 locations along the Tunisian coasts. We concentrated our sampling effort around the Siculo-Tunisian region to verify if any population structuring exists in this region and to identify the factors that have shaped this structure. Measurements of genetic diversity were quantified both within and between populations, and the geographic variability of gene frequencies was analyzed. Our study shows that the Siculo-Tunisian Strait is an important genetic boundary between eastern and western regions, which agrees with findings for a variety of other species. We suggest that vicariance is a predominant factor shaping the current distribution of genetic diversity of R. decussatus, and the mixing of divergent gene pools from the eastern and western regions still seems to be limited by some physical and/or biological factors.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Subscribe and save

Springer+ Basic
$34.99 /Month
  • Get 10 units per month
  • Download Article/Chapter or eBook
  • 1 Unit = 1 Article or 1 Chapter
  • Cancel anytime
Subscribe now

Buy Now

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2

Similar content being viewed by others

References

  • Arnaud-Haond S, Migliaccio M, Diaz-Almela E, Teixeira S, Van de Vliet MS, Alberto F, Procaccini G, Duarte CM, Serrao EA (2007) Vicariance patterns in the Mediterranean Sea: east-west cleavage and low dispersal in the endemic seagrass Posidonia oceanica. J Biogeogr 34:963–976

    Article  Google Scholar 

  • Ayala FJ, Hedgecock D, Zumwalt GS, Valentine JW (1973) Genetic variation in Tridacna maxima, an ecological analog of some unsuccessful evolutionary lineages. Evolution 27:177–191

    Article  Google Scholar 

  • Bahri-Sfar L, Lemaire C, Ben Hassine OK, Bonhomme F (2000) Fragmentation of sea-bass populations in the western and eastern Mediterranean as revealed by microsatellite polymorphism. Proc Roy Soc Lond B 267:929–935

    Article  CAS  Google Scholar 

  • Barton NH, Gale KS (1993) Genetic analysis of hybrid zones. In: Harrison RG (ed) Hybrid zones and the evolutionary process. Oxford University Press, New York, pp 13–45

    Google Scholar 

  • Belkhir K, Borsa P, Chikhi L, Raufaste N, Bonhomme F (2001) Genetix ver. 4.03, logiciel sous Windows la génétique des populations. Laboratoire Génome, Populations, Interactions CNRS UMR 5000, Université de Montpellier II

  • Ben Slimen H, Guerbej H, Ben Othmen A, Ould Brahim I, Blel H, Chatti N, Elabed A, Said K (2004) Genetic differentiation between populations of gilthead sea bream (Sparus aurata) along the Tunisian coast. Cybium 28:45–50

    Google Scholar 

  • Berger EM (1983) Population genetics of marine gastropods and bivalves. Mollusca 6:563–596

    Google Scholar 

  • Borsa P (1997) Intraspecific zoogeography of the Mediterranean: population genetic analysis on sixteen Atlanto-Mediterranean species (fishes and invertebrates). Vie milieu 47:295–305

    Google Scholar 

  • Borsa P, Thiriot-Quiévreux C (1990) Karyological and allozymic characterization of Ruditapes philippinarurn, R. aureus and R. decussatus (Bivalvia: Veneridae). Aquaculture 90:209–227

    Article  Google Scholar 

  • Borsa P, Zainuri M, Delay B (1991) Heterozygote deficiency and population structure in the bivalve Ruditapes decussatus. Heredity 66:1–8

    Article  Google Scholar 

  • Borsa P, Jarne P, Belkhir K, Bonhomme F (1994) Genetic structure of the palourde Ruditapes decussatus L. in the Mediterranean. In: Beaumont AR (ed) Genetics and evolution of aquatic organisms. Chapman and Hall, London, pp 103–113

    Google Scholar 

  • Buroker NE (1982) Allozyme variation in three non-sibling Ostrea species. J Shellfish Res 2:157–163

    Google Scholar 

  • Buroker NE (1983) Population genetics of the American oyster Crassostrea virginica along the Atlantic coast and the Gulf of Mexico. Mar Biol 75:99–112

    Article  Google Scholar 

  • Buroker NE, Hershberger WK, Chew KK (1975) Genetic variation in the Pacific oyster, Crassostrea gigas. J Fish Res Board Can 32:2471–2477

    Article  Google Scholar 

  • Burton RS, Feldman MW (1982) Population genetics of coastal and estuarine invertebrates: does larval behavior influence population structure? In: Kennedy VS (ed) Estuarine comparisons. Academic Press, New York, pp 537–551

    Google Scholar 

  • Cook RM, Sinclair A, Stefansson G (1997) Potential collapse of North Sea cod stocks. Nature 385:521–522

    Article  CAS  Google Scholar 

  • Endler JA (1977) Geographic variation, speciation and clines. Princeton University Press, Princeton

    Google Scholar 

  • Fujio Y (1979) Enzyme polymorphism and population structure of the Pacific oyster, Crassostrea gigas. Tohoku J Agr Res 30:32–42

    CAS  Google Scholar 

  • Gaffney PM, Scott TM, Koehn RK, Diehl WJ (1990) Interrelationships of heterozygosity, growth rate and heterozygote deficiencies in the coot clam, Mulinia lateralis. Genetics 124:687–699

    PubMed  CAS  Google Scholar 

  • Gallardo MH, Penaloza L, Clasing E (1998) Gene flow and allozymic population structure in the clam Venus antiqua from Southern Chile. J Exp Mar Biol Ecol 230:193–205

    Article  Google Scholar 

  • Hewitt GM (1989) The subdivision of species by hybrid zones. In: Otte D, Endler JA (eds) Speciation and its consequences. Sinauer Associates, Sunderland, pp 85–110

    Google Scholar 

  • Hilbish TJ (1985) Demographic and temporal structure of an allele frequency cline in the mussel Mytilus edulis. Mar Biol 86:163–171

    Article  Google Scholar 

  • Hutchings JA (2000) Collapse and recovery of marine fishes. Nature 406:882–885

    Article  PubMed  CAS  Google Scholar 

  • Jarne P, Berrebi P, Guelorget O (1988) Variabilité génétique et morphométrique de cinq populations de la palourde Ruditapes decussatus. Oceanol Acta 11:401–407

    Google Scholar 

  • Jordaens K, De Wolf H, Willems T, Van Dongen S, Brito C, Frias Martins AM, Backeljau T (2000) Loss of genetic variation in a strongly isolated Azorean population of the edible clam, Tapes decussatus. J Shellfish Res 19:29–34

    Google Scholar 

  • Lucas A (1968) Mise en évidence de l’hermaphrodisme juvénile chez Venerupis decussata (L.) (Bivalvia: Veneridae). CR Acad Sci D 367:2332–2333

    Google Scholar 

  • Mantel N (1967) The detection of disease clustering and generalized regression approach. Cancer Res 27:209–220

    PubMed  CAS  Google Scholar 

  • Nei M (1972) Genetic distance between populations. Am Nat 106:283–291

    Article  Google Scholar 

  • Nikula R, Väinölä R (2003) Phylogeography of Cerastoderma glaucum (Bivalvia: Cardiidae) across Europe: a major break in the Eastern Mediterranean. Mar Biol 143:339–350

    Article  Google Scholar 

  • Parache A (1982) La palourde. Pêche Maritime 1254:496–507

    Google Scholar 

  • Passamonti M, Mantovani B, Scali V (1997) Allozymic caracterisation and genetic relationships among four species of Tapetinae. Ital J Zool 64:117–124

    Article  CAS  Google Scholar 

  • Pasteur N, Pasteur G, Bonhomme F, Catalan J, Britton-Davidian J (1986) Manuel d’électrophorèse appliquée à la génétique des populations. Techniques et documentation. Lavoisier, Paris, p 217

    Google Scholar 

  • Pinardi N, Masetti E (2000) Variability of the large scale general circulation of the Mediterranean Sea from observations and modelling: a review. Palaeogeogr Palaeocl 158:153–173

    Article  Google Scholar 

  • Quesada H, Beynon CM, Skibinski DOF (1995) A mitochondrial DNA discontinuity in the mussel Mytilus galloprovincialis Lmk: pleistocene vicariance biogeography and secondary intergradation. Mol Biol Evol 12:521–524

    PubMed  CAS  Google Scholar 

  • Raymond M, Rousset F (2003) Updated version of Genepop (version 1.2): population genetics software for exact tests and ecumenicism. J Hered 86:248–249

    Google Scholar 

  • Rice WR (1989) Analyzing tables of statistical tests. Evolution 43:223–225

    Article  Google Scholar 

  • Riginos C, Cunningham C (2005) Local adaptation and species segregation in two mussels (Mytilus edulis × Mytilus trossulus) hybrid zones. Mar Ecol 14:381–400

    CAS  Google Scholar 

  • Ríos C, Sanz S, Saavedra C, Peña JB (2002) Allozyme variation in populations of scallops, Pecten jacobaeus (L.) and P. maximus (L.) (Bivalvia: Pectinidae), across the Almeria-Oran front. J Exp Mar Biol Ecol 267:223–244

    Article  Google Scholar 

  • Rixen M, Beckers JM, Levitus S, Antonov J, Boyer T, Maillard C, Fichaut M, Balopoulos E, Iona S, Dooley H, Garcia MJ, Manca B, Giorgetti A, Manzella G, Mikhailov N, Pinardi N, Zavatarelli M, The Medar Consortium (2005) The Western Mediterranean deep water: a proxy for climate change. Geophys Res Lett 32:L12608.1–L12608.4

    Article  Google Scholar 

  • Saavedra C, Zapata C, Guerra A, Alvarez G (1993) Allozyme variation in European populations of the oyster Ostrea edulis. Mar Biol 115:85–95

    Article  CAS  Google Scholar 

  • Saavedra C, Zapata C, Alvarez G (1995) Geographical patterns of variability at allozyme loci in the European oyster Ostrea edulis. Mar Biol 122:95–104

    Article  Google Scholar 

  • Sarver SK, Foltz DW (1993) Genetic population structure of a species’ complex of blue mussels (Mytilus spp.). Mar Biol 117:105–112

    Article  Google Scholar 

  • Singh SM, Green RH (1984) Excess of allozyme homozygosity in marine molluscs and its possible biological significance. Malacologia 25:569–581

    Google Scholar 

  • Skibinski DOF, Woodwark M, Ward RD (1993) A quantitative test of the neutral theory using pooled allozyme data. Genetics 135:233–248

    PubMed  CAS  Google Scholar 

  • Theisen BF (1978) Allozyme clines and evidence of strong selection in three loci in Mytilus edulis L. (Bivalvia) from Danish waters. Ophelia 17:135–142

    Google Scholar 

  • Thiede J (1978) A glacial Mediterranean. Nature 276:680–683

    Article  Google Scholar 

  • Thorpe JP, Solé-Cava AM, Watts PC (2000) Exploited marine invertebrates: genetics and fisheries. Hydrobiologia 420:165–184

    Article  Google Scholar 

  • Trigui el Menif N, Le Pennec M, El Abed A (2001) Variabilité génétique de la palourde Ruditapes decussatus de la cote nord-est tunisienne. Haliotis 30:13–22

    Google Scholar 

  • Utter FM (1991) Biochemical genetics and fishery management: an historical perspective. J Fish Biol 39:1–20

    Article  Google Scholar 

  • Väinölä R, Hvilsom MM (1991) Genetic divergence and a hybrid zone between Baltic and North Sea Mytilus populations (Mytilidae: Mollusca). Biol J Linn Soc 43:127–148

    Article  Google Scholar 

  • Weir BS, Cockerham CC (1984) Estimating F-statistics for the analysis of population structure. Evolution 38:1358–1370

    Article  Google Scholar 

  • Wright S (1978) Variability within and among natural populations. In: Evolution and the genetics of populations 4:242–322. University of Chicago Press, Chicago

  • Zitari-Chatti R, Chatti N, Elouaer A, Said K (2008) Genetic variation and population structure of the caramote prawn Penaeus kerathurus from the eastern and western Mediterranean coasts in Tunisia. Aquac Res 39:70–76

    Article  Google Scholar 

  • Zitari-Chatti R, Chatti N, Fulgione D, Caiazza I, Aprea G, El Ouaer A, Said K, Capriglione T (2009) Mitochondrial DNA variation in the caramote prawn Penaeus kerathurus across a transition zone in the Mediterranean sea. Genetica 136:439–447

    Article  PubMed  CAS  Google Scholar 

  • Zouros E, Foltz DF (1984) Minimal selection requirements for the correlation between heterozygosity and growth and for the deficiency of heterozygotes, in oyster populations. Dev Genet 4:393–405

    Article  Google Scholar 

  • Zouros E, Romero-Dorey M, Mallet AL (1988) Heterozygosity and growth in marine bivalves: further data and possible explanations. Evolution 42:1332–1341

    Article  Google Scholar 

Download references

Acknowledgments

We are grateful to Mohamed Nejib Medhioub and to Abderrahman Saidan for the assistance in the collection of the samples from Kneiss and from Kerkenna, respectively. This study was supported by a grant from the Tunisian Ministère de l’Enseignement Supérieur et de la Recherche Scientifique.

Author information

Authors and Affiliations

  1. Unité de Recherche Génétique, Biodiversité et Valorisation des Bioressources, UR03ES09, Institut Supérieur de Biotechnologie de Monastir, Université de Monastir, Av. Tahar Haddad, 5000, Monastir, Tunisia

    Aicha Gharbi, Rym Zitari-Chatti, Khaled Said & Noureddine Chatti

  2. Station de Biologie Marine du Muséum National d’Histoire Naturelle, BP225, 29900, Concarneau, France

    Aicha Gharbi, Alain Van Wormhoudt & Françoise Denis

  3. Institut National des Sciences et Technologies de la Mer (INSTM), Salammbô, Tunisia

    Mohamed Naceur Dhraief

Authors
  1. Aicha Gharbi
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Rym Zitari-Chatti
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Alain Van Wormhoudt
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Mohamed Naceur Dhraief
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Françoise Denis
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Khaled Said
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Noureddine Chatti
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Noureddine Chatti.

 

 

Appendix I Allele frequencies at nine polymorphic loci in 11 populations of Ruditapes decussatus
Full size table

Rights and permissions

Reprints and permissions

About this article

Cite this article

Gharbi, A., Zitari-Chatti, R., Van Wormhoudt, A. et al. Allozyme Variation and Population Genetic Structure in the Carpet Shell Clam Ruditapes decussatus Across the Siculo-Tunisian Strait. Biochem Genet 49, 788–805 (2011). https://doi.org/10.1007/s10528-011-9450-8

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10528-011-9450-8

Keywords

Search

Navigation