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A triple immunochromatographic test for simultaneous determination of cardiac troponin I, fatty acid binding protein, and C-reactive protein biomarkers

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Abstract

The authors describe an immunochromatographic test for the rapid detection of cardiac dysfunction by quantifying the cardiac markers troponin I (TnI), fatty acid binding protein (FABP), and C-reactive protein (CPR) in serum. The dynamics of the formation of the immuno-complexes between antibodies (labeled with gold nanoparticles) and analytes (biomarkers) during immunochromatography was studied, and requirements are specified in terms of membrane carriers and reaction medium that are necessary to detect these biomarkers with their varying intracellular localizations and physical and chemical properties. The test can be performed within 10 min and enables TnI to be determined in concentrations of ≥ 1.0 ng mL−1, FABP in concentrations of ≥ 3.8 ng mL−1, and CPR in concentrations of ≥ 600 ng mL−1. The results of the new test system correlated well with those obtained by ELISAs as used in cardiodiagnostics. It is emphasized here that simultaneous control of several myocardial biomarkers provides more information on the dynamics and development of the pathological process and enables more effective therapeutic decisions.

An immunochromatographic test system for rapid diagnostics of cardiac dysfunctions was developed. In 10 min it provides detection of two biomarker of acute myocardial infarction, namely «rapid» one, fatty acid binding protein and «slow» one, troponin I and also inflammation biomarker, C-reactive protein.

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Acknowledgements

We are grateful to S. M. Pridvorova (A.N. Bach Institute of Biochemistry, Federal Research Center «Fundamentals of Biotechnology») for performing transmission electron microscopy measurements of a gold nanoparticle preparation. This investigation was supported by the Russian Science Foundation project No. 14-14-01131 (the reagents for immunodetection of CRP) and by the Federal Target Program «Development of the Pharmaceutical and Medical Industry of Russia for 2013-2020», contract No. 13411.1008799.13.039 (the reagents for immunodetection of TnI and FABP).

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Authors and Affiliations

  1. A. N. Bach Institute of Biochemistry, Federal Research Center “Fundamentals of Biotechnology”, 119071, Moscow, Russia

    Nadezhda A. Byzova, Anatoly V. Zherdev, Yury Yu. Vengerov & Boris B. Dzantiev

  2. N. I. Pirogov City Clinical Hospital No. 1, 117049, Moscow, Russia

    Тatyana A. Starovoitova

Authors
  1. Nadezhda A. Byzova
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  2. Anatoly V. Zherdev
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  3. Yury Yu. Vengerov
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  4. Тatyana A. Starovoitova
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  5. Boris B. Dzantiev
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Correspondence to Boris B. Dzantiev.

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All serum samples were collected after approval by the local ethics committee and after obtaining written, informed consent from patients.

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Byzova, N.A., Zherdev, A.V., Vengerov, Y.Y. et al. A triple immunochromatographic test for simultaneous determination of cardiac troponin I, fatty acid binding protein, and C-reactive protein biomarkers. Microchim Acta 184, 463–471 (2017). https://doi.org/10.1007/s00604-016-2022-1

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