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ZmMPK17, a novel maize group D MAP kinase gene, is involved in multiple stress responses

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Abstract

Plant mitogen-activated protein kinase (MAPK) cascades play a pivotal role in a range of biotic and abiotic stress responses. In this study, we isolated a novel group D MAPK gene, ZmMPK17, from maize (Zea mays L.). ZmMPK17 is localized mainly to the nucleus and its C-terminal domain extension is believed to be essential for this. Northern-blot analysis indicated that ZmMPK17 transcription is involved in response to exogenous signaling molecules such as abscisic acid, hydrogen peroxide, salicylic acid, jasmonic acid and ethylene and induced by low temperature and osmotic stress. Hydrogen peroxide and Ca2+ mediate PEG-induced downregulation of ZmMPK17 at transcription level and Ca2+ also mediates low temperature-induced expression of ZmMPK17. Overexpression of ZmMPK17 in tobacco (Nicotonia tobaccum) accumulated less reactive oxygen species under osmotic stress by affecting antioxidant defense systems. Transgenic tobacco exhibited enhanced tolerance to cold by means of an increased germination rate, and increased proline and soluble sugar levels relative to control plants. The transcription levels of NtERD10 genes were higher in ZmMPK17-overexpressing lines than in control plants under cold and osmotic stress conditions. ZmMPK17-overexpressing plants displayed enhanced resistance to viral pathogens, and the expression of the pathogenesis-related gene PR1a was significantly increased, indicating that ZmMPK17 might be involved in SA-mediated pathogen defense-signaling pathways.

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Abbreviations

ABA:

Abscisic acid

APX:

Ascorbate peroxidase

CaMV:

Cauliflower mosaic virus

CAT:

Catalase

CD:

C-terminal domain extension

DMTU:

Dimethylthiourea

DAB:

3,3′-Diaminobenzidine

ELISA:

Enzyme-linked immunosorbent assay

GFP:

Green fluorescent protein

H2O2 :

Hydrogen peroxide

JA:

Jasmonic acid

MAPK:

Mitogen-activated protein kinase

NBT:

Nitroblue tetrazolium

POD:

Peroxidase

ROS:

Reactive oxygen species

SA:

Salicylic acid

SOD:

Superoxide dismutase

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Acknowledgments

We thank Lin Ma (Shandong Agricultural University, P.R.China) for providing pathogen and polyclonal antiserum for CMV and PVY detection. Funding for this research was provided by the Grants from the Nation Natural Science Foundation of China (Nos.30871457 and 31071337) and the State Key Basic Research and Development Plan of China (No.2009CB118500).

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Authors and Affiliations

  1. State Key Laboratory of Crop Biology, Shandong Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, 61 Dai Zong Street, Tai’an, 271018, Shandong, China

    Jiaowen Pan, Maoying Zhang, Xiangpei Kong, Xin Xing, Yukun Liu, Yan Zhou, Yang Liu & Dequan Li

  2. Taishan Medical University, Tai’an, 271018, Shandong, China

    Liping Sun

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  1. Jiaowen Pan
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Correspondence to Dequan Li.

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Pan, J., Zhang, M., Kong, X. et al. ZmMPK17, a novel maize group D MAP kinase gene, is involved in multiple stress responses. Planta 235, 661–676 (2012). https://doi.org/10.1007/s00425-011-1510-0

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