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Enhanced chilling tolerance at the booting stage in rice by transgenic overexpression of the ascorbate peroxidase gene, OsAPXa

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Abstract

Low temperatures during the booting stage reduce rice yields by causing cold-induced male sterility. To determine whether antioxidant capacity affects the ability of rice plants to withstand chilling at the booting stage, we produced transgenic rice plants that overexpress OsAPXa and have increased APX activity. The effect of increased APX activity on the levels of H2O2 and lipid peroxidation were determined by measuring H2O2 levels and malondialdehyde (MDA) contents in spikelets during cold treatments at the booting stage. The levels of H2O2 and the MDA content increased by 1.5-fold and twofold, respectively in WT plants subjected to a 12°C treatment for 6 days. In contrast, transgenic lines showed small changes in H2O2 levels and MDA content under cold stress, and H2O2 levels and MDA content were significantly lower than in WT plants. APX activity showed negative correlations with levels of H2O2 and MDA content, which increased during cold treatment. Cold tolerance at the booting stage in transgenic lines and WT plants was evaluated. Spikelet fertility was significantly higher in transgenic lines than in WT plants after a 12°C treatment for 6 days. These results indicate that higher APX activity enhances H2O2-scavenging capacity and protects spikelets from lipid peroxidation, thereby increasing spikelet fertility under cold stress.

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Acknowledgments

We would like to thank Chiyuki Kawata, Noriko Goto, Kazue Sugawara and Sanae Furuhata for technical assistance. This work was supported by a grant from the Ministry of Agriculture, Forestry and Fisheries of Japan (Genomics for Agricultural Innovation, GMA-0009).

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Authors and Affiliations

  1. National Agricultural Research Center for Hokkaido Region, Hitsujigaoka 1, Toyohira, Sapporo, 062-8555, Japan

    Yutaka Sato, Yukari Masuta, Koji Saito & Seiji Murayama

  2. National Institute of Agrobiological Sciences, 2-1-2 Kannondai, Tsukuba, Ibaraki, 305-8602, Japan

    Kenjiro Ozawa

Authors
  1. Yutaka Sato
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  2. Yukari Masuta
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  3. Koji Saito
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  4. Seiji Murayama
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  5. Kenjiro Ozawa
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Corresponding author

Correspondence to Yutaka Sato.

Additional information

Communicated by E. Guiderdoni.

A contribution to the Special Issue: Plant Biotechnology in Support of the Millennium Development Goals.

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Sato, Y., Masuta, Y., Saito, K. et al. Enhanced chilling tolerance at the booting stage in rice by transgenic overexpression of the ascorbate peroxidase gene, OsAPXa . Plant Cell Rep 30, 399–406 (2011). https://doi.org/10.1007/s00299-010-0985-7

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  • DOI: https://doi.org/10.1007/s00299-010-0985-7

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