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Ultrastructural characteristics of Sin Nombre virus, causative agent of hantavirus pulmonary syndrome

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Summary

A previously unrecognized disease, hantavirus pulmonary syndrome, was described following an outbreak of severe, often lethal, pulmonary illness in the southwestern United States in May–June, 1993. We have now studied the morphologic features of the causative agent, Sin Nombre virus (SNV), by thin section electron microscopy and immunoelectron microscopy of infected Vero E6 cells. SNV virions were roughly spherical and had a mean diameter of 112 nm. They had a rather dense envelope and closely apposed fine surface projections, 7 nm in length. Filamentous nucleocapsids were present within virions. Viral inclusion bodies were present in the cytoplasm of infected cells; these appeared granular or filamentous, depending on the plane of section. All of these characteristics were similar to published descriptions of other hantaviruses; however, unlike all other hantaviruses and virtually all other member viruses of the familyBunyaviridae which bud upon smooth intracytoplasmic membranes, SNV budding occurred almost entirely upon the plasma membrane of infected cells. Virus budding was associated with the formation of long 28 nm diameter tubular projections. Occasional elongated 47 nm diameter virus-like particles were seen to bud upon intracytoplasmic membranes. As shown by immunoelectron microscopy, viral antigens were localized over virions, inclusions, and tubular projections associated with virion morphogenesis.

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References

  1. Anderson GW Jr, Smith JF (1987) Immunoelectron microscopy of Rift Valley fever viral morphogenesis in primary rat hepatocytes. Virology 161: 91–100

    Google Scholar 

  2. Bishop DHL, Shope RE (1979)Bunyaviridae. In: Fraenkel-Conrat HL, Wagner RR (eds) Comprehensive virology, vol 14. Plenum, New York, pp 1–156

    Google Scholar 

  3. Casals J (1963) New developments in the classification of arthropod-borne animal viruses. Ann Microbiol 11: 13–34

    Google Scholar 

  4. Centers for Disease Control and Prevention (1993) Outbreak of acute illness — southwestern United States, 1993. MMWR Morb Mortal Wkly Rep 42: 421–424

    Google Scholar 

  5. Centers for Disease Control and Prevention (1994) Newly identified hantavirus — Florida, 1994. MMWR Morb Mortal Wkly Rep 43: 99–105

    Google Scholar 

  6. Childs JE, Ksiazek TG, Spiropoulou CF, Krebs JW, Morzunov S, Maupin GO, Gage KL, Rollin PE, Sarisky J, Enscore RE, Frey J, Peters CJ (1994) Serologic and genetic identification ofPeromyscus maniculatus as the primary rodent reservoir for a new hantavirus in the southwestern United States. J Infect Dis 169: 1271–1280

    Google Scholar 

  7. Donets MA, Chumakov MP, Korolev MB, Rubin SG (1977) Physicochemical characteristics, morphology and morphogenesis of virions of the causative agent of Crimean hemorrhagic fever. Intervirology 8: 294–308

    Google Scholar 

  8. Duchin JS, Koster FT, Peters CJ, Simpson GL, Tempest B, Zaki SR, Ksiazek TG, Rollin PE, Nichol S, Umland ET, Moolenaar R, Reef S, Nolte K, Gallagher M, Butler J, Breiman R, Hantavirus Study Group (1994) Hantavirus pulmonary syndrome: a clinical description of 17 patients with a newly recognized disease. N Engl J Med 330: 949–955

    Google Scholar 

  9. Elliott LH, Ksiazek TG, Rollin PE, Spiropoulou CF, Morzunov S, Monroe M, Goldsmith CS, Humphrey CD, Zaki SR, Krebs JW, Maupin G, Gage K, Childs JE, Nichol ST, Peters CJ (1994) Isolation of the causative agent of hantavirus pulmonary syndrome. Am J Trop Med Hyg 51: 102–108

    Google Scholar 

  10. Fenner F (1975/76) The classification and nomenclature of viruses. Intervirology 6: 1–12

    Google Scholar 

  11. Goldsmith CS, Humphrey CD, Elliott LH, Zaki SR (1994) Morphology of Muerto Canyon virus, causative agent of hantavirus pulmonary syndrome. In: Bailey GW, Garratt-Reed AJ (eds) Proceedings of the Microscopy Society of America Fifty-Second Annual Meeting 1994, San Francisco, pp 272–273

  12. Hung T, Xia S-M, Zhao TX, Zhou JY, Song G, Liao GXH, Ye WW, Chu YL, Hang CS (1983) Morphological evidence for identfying the viruses of hemorrhagic fever with renal syndrome as candidate members of theBunyaviridae family. Arch Virol 78: 137–144

    Google Scholar 

  13. Khan AS, Spiropoulou CF, Morzunov S, Zaki SR, Kohn MA, Nawas SR, McFarland L, Nichol ST (1995) Fatal illness associated with a new hantavirus in Louisiana. J Med Virol 46: 281–286

    Google Scholar 

  14. Ksiazek TG, Peters CJ, Rollin PE, Zaki S, Nichol S, Spiropoulou C, Morzunov S, Feldmann H, Sanchez A, Khan AS, Mahy BWJ, Wachsmuth K, Butler J (1995) Identification of a new North American hantavirus that causes acute pulmonary insufficiency. Am J Trop Med Hyg 52: 117–123

    Google Scholar 

  15. Martin, ML, Lindsey-Regnery H, Sasso DR, McCormick JB, Palmer E (1985) Distinction betweenBunyaviridae genera by surface structure and comparison with Hantaan virus using negative stain electron microscopy. Arch Virol 86: 17–28

    Google Scholar 

  16. Matsuoka Y, Chen S-Y, Compans RW (1994) A signal for Golgi retention in the bunyavirus G1 glycoprotein. J Biol Chem 269: 22565–22573

    Google Scholar 

  17. Mullenhauer HH (1964) Plastic embedding mixtures for use in electron microscopy. Stain Technol 39: 111–114

    Google Scholar 

  18. Murphy FA, Harrison AK, Tzianabos T (1968) Electron microscopic observations of mouse brain infected with Bunyamwera group arboviruses. J Virol 2: 1315–1325

    Google Scholar 

  19. Murphy FA, Harrison AK, Whitfield SG (1973)Bunyaviridae: morphologic and morphogenetic similarities of Bunyamwera serologic supergroup viruses and several other arthropod-borne viruses. Intervirology 1: 297–316

    Google Scholar 

  20. Nichol ST, Spiropoulou CF, Morzunov S, Rollin PE, Ksiazek TG, Feldmann H, Sanchez A, Childs J, Zaki S, Peters CJ (1993) Genetic identification of a hantavirus associated with an outbreak of acute respiratory illness. Science 262: 914–917

    Google Scholar 

  21. Obijeski JF, Bishop DHL, Palmer E, Murphy FA (1976) Segmented genome and nucleocapsid of La Crosse virus. J Virol 20: 664–675

    Google Scholar 

  22. Pettersson RF (1991) Protein localization and virus assembly at intracellular membranes. Curr Top Microbiol Immunol 170: 67–106

    Google Scholar 

  23. Pettersson R, Hewlett MJ, Baltimore D, Coffin JM (1977) The genome of Uukuniemi virus consists of three unique RNA segments. Cell 11: 51–63

    Google Scholar 

  24. Rollin PE, Ksiazek TG, Elliott LH, Ravkov EV, Martin ML, Morzunov S, Livingstone W, Monroe M, Glass G, Ruo S, Khan AS, Childs JE, Nichol ST, Peters CJ (1995) Isolation of Black Creek Canal virus, a new hantavirus fromSigmodon hispidus in Florida. J Med Virol 46: 35–39

    Google Scholar 

  25. Ruo SL, Sanchez A, Elliott LH, Brammer LS, McCormick JB, Fisher-Hoch SP (1991) Monoclonal antibodies to three strains of hantaviruses: Hantaan, R22, and Puumala. Arch Virol 119: 1–11

    Google Scholar 

  26. Saikku P, von Bonsdorff CH, Brummer-Korvenknotio M, Vaheri A (1971) Isolation of non-cuboidal ribonucleoprotein from Inkoo virus, a Bunyamwera supergroup arbovirus. J Gen Virol 13: 335–337

    Google Scholar 

  27. Schmaljohn AL, Li D, Negley DL, Bressler DS, Turell MJ, Korch GW, Ascher MS, Schmaljohn CS (1995) Isolation and initial characterization of a newfound hantavirus from California. Virology 206: 963–972

    Google Scholar 

  28. Schmaljohn CS, Beaty BJ, Calisher CH, Dalrymple JM, Elliott RM, Karabatsos N, Kolakofsky K, Lee HW, Lvov DK, Marriott AC, Nuttall PA, Peters D, Pettersson RF, Shope RE (1995)Bunyaviridae. In: Murphy FA, Fauquet CM, Bishop DHL, Ghabrial SA, Jarvis AW, Martelli GP, Mayo MA, Summers MD (eds) Virus Taxonomy. Classification and Nomenclature of Viruses. Sixth Report of the International Committee on Taxonomy of Viruses. Springer, Wien New York, pp 300–315 (Arch Virol [Suppl] 10)

    Google Scholar 

  29. Schmaljohn CS, Patterson JL (1990) Bunyaviridae and their replication. In: Fields BN, Knipe DM (eds) Fields Virology. Raven Press, New York, pp 1175–1194

    Google Scholar 

  30. Spiropoulou CF, Morzunov S, Feldmann H, Sanchez A, Peters CJ, Nichol ST (1994) Genome structure and variability of a virus causing hantavirus pulmonary syndrome. Virology 200: 715–723

    Google Scholar 

  31. Stevenson AE, Holmes IH (1972) Electron microscopy of Koongol group arboviruses. Aust J Biol Sci 25: 53–60

    Google Scholar 

  32. Tao H (1988) Atlas of hemorrhagic fever with renal syndrome. Science Press, Beijing

    Google Scholar 

  33. Tao H, Semao X, Zinyi C, Gan S, Yanagihara R (1987) Morphology and morphogenesis of viruses of hemorrhagic fever with renal syndrome: II. Inclusion bodies — ultrastructural markers of hantavirus-infected cells. Intervirology 27: 45–52

    Google Scholar 

  34. Tao H, Zinyi C, Tungxin Z, Semao X, Changshou H (1985) Morphology and morphogenesis of viruses of hemorrhagic fever with renal syndrome (HFRS): I. Some peculiar aspects of the morphogenesis of various strains of HFRS virus. Intervirology 23: 97–108

    Google Scholar 

  35. Zaki SR, Greer PW, Coffield LM, Goldsmith CS, Nolte KB, Foucar K, Feddersen RM, Zumwalt RE, Miller GL, Khan AS, Rollin PE, Ksiazek TG, Nichol ST, Mahy BWJ, Peters CJ (1995) Hantavirus pulmonary syndrome: pathogenesis of an emerging infectious disease. Am J Pathol 146: 552–579

    Google Scholar 

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Authors and Affiliations

  1. Division of Viral and Rickettsial Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia, USA

    C. S. Goldsmith, L. H. Elliott, C. J. Peters & S. R. Zaki

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  1. C. S. Goldsmith
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  2. L. H. Elliott
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  3. C. J. Peters
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  4. S. R. Zaki
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Goldsmith, C.S., Elliott, L.H., Peters, C.J. et al. Ultrastructural characteristics of Sin Nombre virus, causative agent of hantavirus pulmonary syndrome. Archives of Virology 140, 2107–2122 (1995). https://doi.org/10.1007/BF01323234

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