Abstract
We present an image driven approach to the reconstruction of 3-D volumes from stacks of 2-D post-mortem sections (histology, cryoimaging, autoradiography or immunohistochemistry) in the absence of any external information. We note that a desirable quality of the reconstructed volume is the smoothness of its notable structures (e.g. the gray/white matter surfaces in brain images). Here we propose to use smoothness as a means to drive the reconstruction process itself.
From an initial rigid pair-wise reconstruction of the input 2-D sections, we extract the boundaries of structures of interest. Those are then evolved under a mean curvature flow modified to constrain the flow within 2-D planes. Sparse displacement fields are then computed, independently for each slice, from the resulting flow. A variety of transformations, from globally rigid to arbitrarily flexible ones, can then be estimated from those fields and applied to the individual input 2-D sections to form a smooth volume.
We detail our method and discuss preliminary results on both real histological data and synthetic examples.
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Cifor, A., Pridmore, T., Pitiot, A. (2009). Smooth 3-D Reconstruction for 2-D Histological Images. In: Prince, J.L., Pham, D.L., Myers, K.J. (eds) Information Processing in Medical Imaging. IPMI 2009. Lecture Notes in Computer Science, vol 5636. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-02498-6_29
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DOI: https://doi.org/10.1007/978-3-642-02498-6_29
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