Abstract
Multiple stains are usually used to highlight biological substances in biomedical image analysis. To decompose multiple stains for co-localization quantification, blind source separation is usually performed. Prior model-based stain separation methods usually rely on stains’ spatial distributions over an image and may fail to solve the co-localization problem. With the advantage of machine learning, deep generative models are used for this purpose. Since prior knowledge of imaging models is ignored in purely data-driven solutions, these methods may be sub-optimal. In this study, a novel learning-based blind source separation framework is proposed, where the physical model of biomedical imaging is incorporated to regularize the learning process. The introduced model-relevant adversarial loss couples all generators in the framework and limits the capacities of the generative models. Further more, a training algorithm is innovated for the proposed framework to avoid inter-generator confusion during learning. This paper particularly takes fluorescence unmixing in fluorescence microscopy images as an application example of the proposed framework. Qualitative and quantitative experimentation on a public fluorescence microscopy image set demonstrates the superiority of the proposed method over both prior model-based approaches and learning-based methods.
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Li, X. (2021). Blind Stain Separation Using Model-Aware Generative Learning and Its Applications on Fluorescence Microscopy Images. In: Feragen, A., Sommer, S., Schnabel, J., Nielsen, M. (eds) Information Processing in Medical Imaging. IPMI 2021. Lecture Notes in Computer Science(), vol 12729. Springer, Cham. https://doi.org/10.1007/978-3-030-78191-0_8
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