Abstract
Hoffman microscopy imaging systems are part of numerous fertility clinics world-wide. We discuss the physics of the Hoffman imaging system from optical thickness to image intensity, implement a simple, yet fast, reconstruction algorithm using Fast Fourier Transformation and discuss the usability of the method on a number of cells from a human embryo. Novelty is identifying the non-linearity of a typical Hoffman imaging system, and the application of Fourier Transformation to reconstruct the optical thickness.
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Olsen, N.H., Sporring, J., Nielsen, M., Hnida, C., Ziebe, S. (2003). Reconstructing the Optical Thickness from Hoffman Modulation Contrast Images. In: Bigun, J., Gustavsson, T. (eds) Image Analysis. SCIA 2003. Lecture Notes in Computer Science, vol 2749. Springer, Berlin, Heidelberg. https://doi.org/10.1007/3-540-45103-X_70
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DOI: https://doi.org/10.1007/3-540-45103-X_70
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