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Salivary markers and risk factor data: A multivariate modeling approach for head and neck squamous cell carcinoma detection

Article type: Research Article

Authors: Pereira, Lutécia H. Mateusa | Adebisi, Islamiyat Nancya | Perez, Aymeeb | Wiebel, Michaelc | Reis, Isildinhad; e | Duncan, Robertd | Goodwin, W. Jarrarda; c | Hu, Jennifer J.a; d | Lokeshwar, Vinata B.b; f | Franzmann, Elizabeth J.a; c; *

Affiliations: [a] Sylvester Comprehensive Cancer Center, University of Miami Leonard Miller School of Medicine, Miami, FL, USA | [b] Department of Cell Biology and Anatomy, University of Miami Leonard Miller School of Medicine, Miami, FL, USA | [c] Department of Otolaryngology, University of Miami Leonard Miller School of Medicine, Miami, FL, USA | [d] Division of Biostatistics, Department of Epidemiology and Public Health, University of Miami Leonard Miller School of Medicine, Miami, FL, USA | [e] Sylvester Comprehensive Cancer Center, Division of Biostatistics, University of Miami Leonard Miller School of Medicine, Miami, FL, USA | [f] Department of Urology, University of Miami Leonard Miller School of Medicine, Miami, FL, USA

Correspondence: [*] Corresponding author: Elizabeth J. Franzmann, Department of Otolaryngology, Head and Neck Surgery Division, University of Miami, Leonard Miller School of Medicine, and Jackson Memorial Hospital, 1120 NW 14th Street, Clinical Research Bldg, Room 1513, Miami, FL 33136, USA. Tel.: +1 305 243 5955; Fax: +1 305 243 3383; E-mail: [email protected].

Keywords: CD44, hyaluronic acid, IL-8, protein, head and neck cancer

DOI: 10.3233/CBM-2012-0252

Journal: Cancer Biomarkers, vol. 10, no. 5, pp. 241-249, 2012

Published: 8 June 2012
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Abstract

Background:

Head and neck squamous cell carcinoma (HNSCC) is a debilitating and deadly disease largely due to late stage diagnosis. Prior work indicates that soluble CD44 (solCD44) and total protein may be useful diagnostic markers for HNSCC. In this study we combine the markers solCD44, IL-8, HA, and total protein with demographic and risk factor data to derive a multivariate logistic model that improves HNSCC detection as compared to our previous data using biomarkers alone.

Methods:

We performed the solCD44, IL-8, HA, and total protein assays on oral rinses from 40 HNSCC patients and 39 controls using ELISA assays. Controls had benign diseases of the upper aerodigestive tract and a history of tobacco or alcohol use. All subjects completed a questionnaire including demographic and risk factor data.

Results:

Depending on cancer subsite, differences between cases and controls were found for all markers. A multivariate logistic model including solCD44, total protein and variables related to smoking, oral health and education offered a significant improvement over the univariate models with an AUC of 0.853. Sensitivity ranged from 75–82.5% and specificity from 69.2âĂŞ82.1% depending on predictive probability cut points.

Conclusion:

A multivariate model, including simple and inexpensive molecular tests in combination with risk factors, results in a promising tool for distinguishing HNSCC patients from controls.

Impact:

In this case-control study, the resulting observations led to an unprecedented multivariate model that distinguished HNSCC cases from controls with better accuracy than the current gold standard which includes oral examination followed by tissue biopsy. Since the components are simple, noninvasive, and inexpensive to obtain, this model combining biomarkers, risk factor and demographic data serves as a promising prototype for future cancer detection tests.

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