Hyperthermophile

General physiology

Due to their extreme environments, hyperthermophiles can be adapted to several variety of factors such as pH, redox potential, level of salinity, and temperature. They grow (similar to mesophiles) within a temperature range of about 25–30 °C between the minimal and maximal temperature. The fastest growth is obtained at their optimal growth temperature which may be up to 106 °C.^[10] The main characteristics they present in their morphology are:

• Cell wall: the outermost part of archaea, it is arranged around the cell and protects the cell contents. It does not contain peptidoglycan, which makes them naturally resistant to lysozyme. The most common wall is a paracrystalline surface layer formed by proteins or glycoproteins of hexagonal symmetry. An exception is the genus Thermoplasma which lacks a wall, a deficiency that is filled by the development of a cell membrane with a unique chemical structure, containing a lipid tetraether unit and glucose in a very high proportion to the total lipids. In addition, it is accompanied by glycoproteins that together with lipids give the membrane of Thermoplasma species stability against the acidic and thermophilic conditions in which it lives.^[11]
• Cytoplasmic membrane: is the main adaptation to temperature. This membrane is radically different from that known from eukaryotes. The membrane of Archaea is built on a tetraether unit, thus establishing ether bonds between glycerol molecules and hydrophobic side chains that do not consist of fatty acids. These side chains are mainly composed of repeating isoprene units.^[11] At certain points of the membrane, side chains linked by covalent bonds and a monolayer are found at these points. Thus, the membrane is much more stable and resistant to temperature alterations than the acidic bilayers present in eukaryotic organisms and bacteria.
• Proteins: denature at elevated temperatures and so also must adapt. Protein complexes known as heat shock proteins assist with proper folding. Their function is to bind or engulf the protein during synthesis, creating an environment conducive to its correct tertiary conformation. In addition, heat shock proteins can collaborate in transporting newly folded proteins to their site of action.^[11]
• DNA: is also adapted to elevated temperatures by several mechanisms. The first is cyclic potassium 2,3-diphosphoglycerate, which has been isolated in only a few species of the genus. Methanopyrus is characterized by the fact that it prevents DNA damage at these temperatures.^[10] Topoisomerase is an enzyme found in all hyperthermophiles. It is responsible for the introduction of positive spins which confer greater stability against high temperatures. Sac7d this protein has been found in the genus and characterized by an increase, up to 40 °C, in the melting temperature of DNA. The histones with which these proteins are associated collaborate in its supercoiling.^[12][10]

Adaptations

As a rule, hyperthermophiles do not propagate at 50 °C or below, some not even below 80 or 90º.^[16] Although unable to grow at ambient temperatures, they are able to survive there for many years. Based on their simple growth requirements, hyperthermophiles could grow in any hot water-containing site, potentially even on other planets and moons like Mars and Europa. Thermophiles and hyperthermophiles employ different mechanisms to adapt their cells to heat, especially to the cell wall, plasma membrane, and its biomolecules (DNA, proteins, etc.):^[12]

• The presence in their plasma membrane of long-chain and saturated fatty acids in bacteria and "ether" bonds (diether or tetraether) in archaea. In some archaea the membrane has a monolayer structure which further increases its heat resistance.
• Overexpression of GroES and GroEL chaperones that help the correct folding of proteins in situations of cellular stress such as the temperature in which they grow.
• Accumulation of compounds such as potassium diphosphoglycerate that prevent chemical damage (depurination or depyrimidination) to DNA.
• Production of spermidine that stabilizes DNA, RNA and ribosomes.
• Presence of a DNA reverse DNA gyrase that produces positive supercoiling and stabilizes DNA against heat.
• Presence of proteins with higher content in α-helix regions, more resistant to heat.

DNA repair

The hyperthermophilic archaea appear to have special strategies for coping with DNA damage that distinguish these organisms from other organisms.^[17] These strategies include an essential requirement for key proteins employed in homologous recombination (a DNA repair process), an apparent lack of the DNA repair process of nucleotide excision repair, and a lack of the MutS/MutL homologs (DNA mismatch repair proteins).^[17]

Archaea

• Strain 121, an archaeon living at 121 °C in the Pacific Ocean.
• Pyrolobus fumarii, an archaeon living at 113 °C in Atlantic hydrothermal vents.
• Pyrococcus furiosus, an archaeon which thrives at 100 °C, first discovered in Italy near a volcanic vent.
• Archaeoglobus fulgidus
• Methanococcus jannaschii
• Aeropyrum pernix
• Sulfolobus
• Methanopyrus kandleri strain 116, an archaeon in 80–122 °C in a Central Indian Ridge.

Gram-negative Bacteria

• Aquifex aeolicus
• Geothermobacterium ferrireducens, which thrives in 65–100 °C in Obsidian Pool, Yellowstone National Park.
• Thermotoga, especially Thermotoga maritima