Field stain

Field stain is a histological method for staining of blood smears. It is used for staining thick blood films in order to discover malarial parasites. Field's stain is a version of a Romanowsky stain, used for rapid processing of the specimens.^[1]

Thumb — Micrograph of a Field stain showing malignant melanoma.

Field's stain consists of two parts - Field's stain A is methylene blue and Azure 1 dissolved in phosphate buffer solution; Field's stain B is Eosin Y in buffer solution. Field stain is named after physician John William Field, who developed it in 1941.^[2]

Colorectal adenocarcinoma. Field stain.
Granuloma. Field stain.

[1]
Chunge, CN.; Ngige, S.; Bwibo, CR.; Mulega, PC.; Kilonzo, JF.; Kibati, F.; Owate, J. (Aug 1989). "A rapid staining technique for Leishmania parasites in splenic aspirate smears". Ann Trop Med Parasitol. 83 (4): 361–4. doi:10.1080/00034983.1989.11812358. PMID 2481429.
[2]
"Obituaries" (PDF). Medical Journal of Malaysia. June 1981. Retrieved 4 May 2015.

Field stain recipe (thelabrat.com)

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[pmid2481429-1] [1]
Chunge, CN.; Ngige, S.; Bwibo, CR.; Mulega, PC.; Kilonzo, JF.; Kibati, F.; Owate, J. (Aug 1989). "A rapid staining technique for Leishmania parasites in splenic aspirate smears". Ann Trop Med Parasitol. 83 (4): 361–4. doi:10.1080/00034983.1989.11812358. PMID 2481429.

[2] [2]
"Obituaries" (PDF). Medical Journal of Malaysia. June 1981. Retrieved 4 May 2015.

[1]

[2]

Field stain

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