Antonie van Leeuwenhoek

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Antonie Philips van Leeuwenhoek^{[note 2]} FRS (/ˈɑːntəni vɑːn ˈleɪvənhuːk, -hʊk/ AHN-tə-nee vahn LAY-vən-hook, -⁠huuk; Dutch: [ˈɑntoːni vɑn ˈleːu.ə(n)ˌɦuk] ^ⓘ; 24 October 1632 – 26 August 1723) was a Dutch microbiologist and microscopist in the Golden Age of Dutch science and technology. A largely self-taught man in science, he is commonly known as "the Father of Microbiology", and one of the first microscopists and microbiologists.^[5][6][7][8] Van Leeuwenhoek is best known for his pioneering work in microscopy and for his contributions toward the establishment of microbiology as a scientific discipline.

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Antonie van Leeuwenhoek FRS
Portrait by Jan Verkolje, after 1680
Born	(1632-10-24)24 October 1632 Delft, Dutch Republic
Died	26 August 1723(1723-08-26) (aged 90) Delft, Dutch Republic
Known for	First acknowledged microscopist and microbiologist in history[note 1] Microscopic discovery of microorganisms (animalcule)
Scientific career
Fields	Microscopy Microbiology
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Raised in Delft, Dutch Republic, Van Leeuwenhoek worked as a draper in his youth and founded his own shop in 1654. He became well-recognized in municipal politics and developed an interest in lensmaking. In the 1670s, he started to explore microbial life with his microscope.

Using single-lensed microscopes of his own design and make, Van Leeuwenhoek was the first to observe and to experiment with microbes, which he originally referred to as dierkens, diertgens or diertjes.^{[note 3]} He was the first to relatively determine their size. Most of the "animalcules" are now referred to as unicellular organisms, although he observed multicellular organisms in pond water. He was also the first to document microscopic observations of muscle fibers, bacteria, spermatozoa, red blood cells, crystals in gouty tophi, and among the first to see blood flow in capillaries. Although Van Leeuwenhoek did not write any books, he described his discoveries in chaotic letters to the Royal Society, which published many of his letters in their Philosophical Transactions.^[10]

Antonie van Leeuwenhoek was born in Delft, Dutch Republic, on 24 October 1632. On 4 November, he was baptized as Thonis. His father, Philips Antonisz van Leeuwenhoek, was a basket maker who died when Antonie was only five years old. His mother, Margaretha (Bel van den Berch), came from a well-to-do brewer's family. She remarried Jacob Jansz Molijn, a painter and the family moved to Warmond around 1640. Antonie had four older sisters: Margriet, Geertruyt, Neeltje, and Catharina.^[11] When he was around ten years old his step-father died. He was sent to live in Benthuizen with his uncle, an attorney. At the age of 16 he became a bookkeeper's apprentice (casher) at a linen-draper's shop at Warmoesstraat in Amsterdam,^[12] which was owned by William Davidson. Van Leeuwenhoek left there after six years.^[13][14]

In July 1654, Van Leeuwenhoek married Barbara de Mey in Delft, with whom he fathered one surviving daughter, Maria (four other children died in infancy). He would live and study for the rest of his life at Hypolytusbuurt in a house he bought in 1655. He opened a draper's shop, selling linen, yarn and ribbon to seamstresses and tailors.^[15] His status in Delft grew throughout the years. In 1660 he received a lucrative job as chamberlain for the sheriffs in the city hall, a position which he would hold for almost 40 years. His duties included maintaining the premises, heating, cleaning, opening for meetings, performing duties for those assembled, and maintaining silence on all matters discussed there.

In 1669 he was appointed as a land surveyor by the court of Holland; at some time he combined it with another municipal job, being the official "wine-gauger" of Delft and in charge of the city wine imports and taxation.^[16] His wife had died in 1666, and in 1671, Van Leeuwenhoek remarried to Cornelia Swalmius with whom he had no children.^[17]

Van Leeuwenhoek was a contemporary of another famous Delft citizen, the painter Johannes Vermeer, who was baptized just four days earlier. It has been suggested that he is the man portrayed in two Vermeer paintings of the late 1660s, The Astronomer and The Geographer, but others argue that there appears to be little physical similarity. Because they were both relatively important men in a city with only 24,000 inhabitants, living both close to the main market, it is likely they knew each other. Van Leeuwenhoek acted as the executor of Vermeer's will when the painter died in 1675.^{[18][note 4]}

Van Leeuwenhoek's religion was "Dutch Reformed" and Calvinist.^[19] Like Jan Swammerdam he often referred with reverence to the wonders God designed in making creatures great and small, and believed that his discoveries were merely further proof of the wonder of creation.^[20][21]

While running his draper shop, Van Leeuwenhoek wanted to see the quality of the thread better than what was possible using the magnifying lenses of the time. He developed an interest in lensmaking, although few records exist of his early activity. By placing the middle of a small rod of soda lime glass in a hot flame, one can pull the hot section apart to create two long whiskers of glass. Then, by reinserting the end of one whisker into the flame, a very small, high-quality glass lens is created. Significantly, a May 2021 neutron tomography study of a high-magnification Leeuwenhoek microscope^[22] captured images of the short glass stem characteristic of this lens creation method. For lower magnifications he also made ground lenses.^[23] To help keep his methods confidential he apparently intentionally encouraged others to think grinding was his primary or only lens construction method.^{[citation needed]}

Recognition by the Royal Society

After developing his method for creating powerful lenses and applying them to the study of the microscopic world,^[24] Van Leeuwenhoek introduced his work to his friend, the prominent Dutch physician Reinier de Graaf. When the Royal Society in London published the groundbreaking work of an Italian lensmaker in their journal Philosophical Transactions of the Royal Society, de Graaf wrote to the editor of the journal, Henry Oldenburg, with a ringing endorsement of Van Leeuwenhoek's microscopes which, he claimed, "far surpass those which we have hitherto seen". In response, in 1673 the society published a letter from Van Leeuwenhoek that included his microscopic observations on mold, bees, and lice.^[25] Then, in 1674, Van Leeuwenhoek made his most significant discovery. Starting from the assumption that life and motility are similar, he determined that the moving objects observed under his microscope were little animals. He later recorded his observations in his diary.^[26]

Van Leeuwenhoek's work fully captured the attention of the Royal Society, and he began corresponding regularly with the society regarding his observations. At first he had been reluctant to publicize his findings, regarding himself as a businessman with little scientific, artistic, or writing background, but de Graaf urged him to be more confident in his work.^[27] By the time Van Leeuwenhoek died in 1723, he had written some 190 letters to the Royal Society, detailing his findings in a wide variety of fields, centered on his work in microscopy. He only wrote letters in his own colloquial Dutch; he never published a proper scientific paper in Latin. He strongly preferred to work alone, distrusting the sincerity of those who offered their assistance.^[28] The letters were translated into Latin or English by Henry Oldenburg, who had learned Dutch for this very purpose.^{[citation needed]} He was also the first to use the word animalcules to translate the Dutch words that Leeuwenhoek used to describe microorganisms.^[9] Despite the initial success of Van Leeuwenhoek's relationship with the Royal Society, soon relations became severely strained. His credibility was questioned when he sent the Royal Society a copy of his first observations of microscopic single-celled organisms dated 9 October 1676.^[29] Previously, the existence of single-celled organisms was entirely unknown. Thus, even with his established reputation with the Royal Society as a reliable observer, his observations of microscopic life were initially met with some skepticism.^[30]

Eventually, in the face of Van Leeuwenhoek's insistence, the Royal Society arranged for Alexander Petrie, minister to the English Reformed Church in Delft; Benedict Haan, at that time Lutheran minister at Delft; and Henrik Cordes, then Lutheran minister at the Hague, accompanied by Sir Robert Gordon and four others, to determine whether it was in fact Van Leeuwenhoek's ability to observe and reason clearly, or perhaps, the Royal Society's theories of life that might require reform. Finally in 1677,^[31] Van Leeuwenhoek's observations were fully acknowledged by the Royal Society.^[32]

Antonie van Leeuwenhoek was elected to the Royal Society in February 1680 on the nomination of William Croone, a then-prominent physician.^{[note 5]} Van Leeuwenhoek was "taken aback" by the nomination, which he considered a high honour, although he did not attend the induction ceremony in London, nor did he ever attend a Royal Society meeting.^[34] He had his portrait painted by Jan Verkolje with the certificate signed by James II of England on the table beside him.

Antonie van Leeuwenhoek made more than 500 optical lenses. He also created at least 25 single-lens microscopes, of differing types, of which only nine have survived. These microscopes were made of silver or copper frames, holding hand-made lenses. Those that have survived are capable of magnification up to 275 times. It is suspected that Van Leeuwenhoek possessed some microscopes that could magnify up to 500 times. Although he has been widely regarded as a dilettante or amateur, his scientific research was of remarkably high quality.^{[40][citation needed]}

The single-lens microscopes of Van Leeuwenhoek were relatively small devices, the largest being about 5 cm long.^[41][42] They are used by placing the lens very close in front of the eye. The other side of the microscope had a pin, where the sample was attached in order to stay close to the lens. There were also three screws to move the pin and the sample along three axes: one axis to change the focus, and the two other axes to navigate through the sample.

Van Leeuwenhoek maintained throughout his life that there are aspects of microscope construction "which I only keep for myself", in particular his most critical secret of how he made the lenses.^[43] For many years no one was able to reconstruct Van Leeuwenhoek's design techniques, but, in 1957, C.L. Stong used thin glass thread fusing instead of polishing, and successfully created some working samples of a Van Leeuwenhoek design microscope.^[44] Such a method was also discovered independently by A. Mosolov and A. Belkin at the Russian Novosibirsk State Medical Institute.^[45] In May 2021, researchers in the Netherlands published a non-destructive neutron tomography study of a Leeuwenhoek microscope.^[22] One image in particular shows a Stong/Mosolov-type spherical lens with a single short glass stem attached (Fig. 4). Such lenses are created by pulling an extremely thin glass filament, breaking the filament, and briefly fusing the filament end. The nuclear tomography article notes this lens creation method was first devised by Robert Hooke rather than Leeuwenhoek, which is ironic given Hooke's subsequent surprise at Leeuwenhoek's findings.

Van Leeuwenhoek used samples and measurements to estimate numbers of microorganisms in units of water.^[46][47] He also made good use of the huge advantage provided by his method. He studied a broad range of microscopic phenomena, and shared the resulting observations freely with groups such as the British Royal Society.^[48] Such work firmly established his place in history as one of the first and most important explorers of the microscopic world. Van Leeuwenhoek was one of the first people to observe cells, much like Robert Hooke.^[6] He also corresponded with Antonio Magliabechi.^[49]

• Leeuwenhoek was one of the first to conduct experiments on himself. It was from his finger that blood was drawn for examination, and he placed pieces of his skin under a microscope, examining its structure in various parts of the body, and counting the number of vessels that permeate it.^[50]
• Both Marcello Malpighi and Jan Swammerdam saw these structures before Leeuwenhoek, but Leeuwenhoek was the first to recognize what they are: red blood cells.^[51]
• Infusoria (protists in modern zoological classification), in 1674
• In 1675, he was studying a variety of minerals, especially salts, and parts of plants and animals.
• The vacuole of the cell in 1676
• Spermatozoa, in 1677
• The banded pattern of muscular fibers, in 1682
• Bacteria, (e.g., large Selenomonads from the human mouth), in 1683^{[52][note 6][53][note 7]}
• It seems he used horseradish to find out what causes irritation on the tongue.^[54] He used the effect of vinegar.
• Leeuwenhoek diligently began to search for his animalcules.^[50] He found them everywhere: in rotten water, in ditches, on his own teeth. "Although I am now fifty years old," he wrote to the Royal Society, "my teeth are well preserved, because I am in the habit of rubbing them with salt every morning." He described paradontitis.^[55]
• In 1684 he published his research on the ovary.^[56]
• In 1687, Van Leeuwenhoek reported his research on the coffee bean.^[57][58] He roasted the bean, cut it into slices and saw a spongy interior. The bean was pressed, and an oil appeared. He boiled the coffee with rain water twice and set it aside.^[59]
• Leeuwenhoek corresponded regularly with Anthonie Heinsius, the Delft pensionary in the States of Holland and in 1687 member of the board of the Delft chamber of the VOC.
• In 1696 Nicolaas Witsen sent him a map of Tartary and ore found near the Amur in Siberia.^[60]

• Van Leeuwenhoek has been recognized as the first person to use a histological stain to color specimens observed under the microscope using saffron.^[61] He used this technique only once.^[62][63]

• In 1702 he requested a book on Peruvian silver mines in Potosí.

Like Robert Boyle and Nicolaas Hartsoeker, Van Leeuwenhoek was interested in dried cochineal, trying to find out if the dye came from a berry or an insect.^{[64][65][66][67]}

He studied rainwater, the seeds of oranges, worms in sheep's liver, the eye of a whale, the blood of fishes, mites, coccinellidae, the skin of elephants, Celandine, and Cinchona.^[49]

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  Van Leeuwenhoek's microscopes by Henry Baker
• 
  Leeuwenhoek Boerhaave museum
• 
  A replica of a microscope by Van Leeuwenhoek

By the end of his life, Van Leeuwenhoek had written approximately 560 letters to the Royal Society and other scientific institutions concerning his observations and discoveries. Even during the last weeks of his life, Van Leeuwenhoek continued to send letters full of observations to London. The last few contained a precise description of his own illness. He suffered from a rare disease, an uncontrolled movement of the midriff, which now is named Van Leeuwenhoek's disease.^[68] He died at the age of 90, on 26 August 1723, and was buried four days later in the Oude Kerk in Delft.^[69]

In 1981, the British microscopist Brian J. Ford found that Van Leeuwenhoek's original specimens had survived in the collections of the Royal Society of London. They were found to be of high quality, and all were well preserved.^[70][71][72] Ford carried out observations with a range of single-lens microscopes, adding to our knowledge of Van Leeuwenhoek's work.^[73] In Ford's opinion, Leeuwenhoek remained imperfectly understood, the popular view that his work was crude and undisciplined at odds with the evidence of conscientious and painstaking observation. He constructed rational and repeatable experimental procedures and was willing to oppose received opinion, such as spontaneous generation, and he changed his mind in the light of evidence.^[40]

On his importance in the history of microbiology and science in general, the British biochemist Nick Lane wrote that he was "the first even to think of looking—certainly, the first with the power to see." His experiments were ingenious, and he was "a scientist of the highest calibre", attacked by people who envied him or "scorned his unschooled origins", not helped by his secrecy about his methods.^[30]

The Antoni van Leeuwenhoek Hospital in Amsterdam, named after Van Leeuwenhoek, is specialized in oncology.^[74] In 2004, a public poll in the Netherlands to determine the greatest Dutchman ("De Grootste Nederlander") named Van Leeuwenhoek the 4th-greatest Dutchman of all time.^[75]

On 24 October 2016, Google commemorated the 384th anniversary of Van Leeuwenhoek's birth with a Doodle that depicted his discovery of "little animals" or animalcules, now known as unicellular organisms.^[76]

The Leeuwenhoek Medal, Leeuwenhoek Lecture, Leeuwenhoek crater, Leeuwenhoeckia, Levenhookia (a genus in the family Stylidiaceae), Leeuwenhoekiella (an aerobic bacterial genus), and the scientific publication Antonie van Leeuwenhoek: International Journal of General and Molecular Microbiology are named after him.^[77]

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  Memorial of Antonie van Leeuwenhoek in the Oude Kerk in Delft
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  Antonie van Leeuwenhoek is buried in the Oude Kerk.
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  Het Gouden Hoofd (Hippolytusbuurt 1–3, Delft).^[78]

• Animalcule
• Regnier de Graaf
• Dutch Golden Age
• History of microbiology
• Microscopy
• Microscope
• Robert Hooke
• Microscopic discovery of microorganisms
• Microscopic scale
• Science and technology in the Dutch Republic
• Scientific Revolution
• Nicolas Steno
• Jan Swammerdam
• Timeline of microscope technology
• Johannes Vermeer