NSF Antibody (A-3): sc-515043
- NSF Antibody (A-3) is a mouse monoclonal IgG2a κ NSF antibody provided at 200 µg/ml
- specific for an epitope mapping between amino acids 50-69 near the N-terminus of NSF of human origin
- NSF Antibody (A-3) is recommended for detection of NSF of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-NSF Antibody (A-3) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of NSF (A-3): sc-515043.
- m-IgG Fc BP-HRP, 2a BP-HRP">m-IgG2a BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for NSF Antibody (A-3) for WB applications. These reagents are now offered in bundles with NSF Antibody (A-3) (see ordering information below).
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NSF Antibody (A-3) is a mouse monoclonal IgG2a kappa light chain antibody that detects NSF protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-NSF antibody (A-3) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. NSF (A-3) antibody recognizes a protein that plays a crucial role in regulating membrane fusion processes, particularly in exocytosis, where NSF is essential for disassembling SNARE complexes vital for vesicle fusion with target membranes. By facilitating SNARE protein recycling, NSF ensures efficient operation of cellular machinery responsible for neurotransmitter release and other membrane trafficking events. NSF (A-3) monoclonal antibody serves as an invaluable tool for researchers studying exocytosis dynamics and molecular mechanisms underlying synaptic function, providing insights into both normal physiology and potential pathologies associated with synaptic dysfunction.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
NSF Antibody (A-3) References:
- The syntaxin family of vesicular transport receptors. | Bennett, MK., et al. 1993. Cell. 74: 863-73. PMID: 7690687
- Synaptobrevin binding to synaptophysin: a potential mechanism for controlling the exocytotic fusion machine. | Edelmann, L., et al. 1995. EMBO J. 14: 224-31. PMID: 7835333
- Synaptic core complex of synaptobrevin, syntaxin, and SNAP25 forms high affinity alpha-SNAP binding site. | McMahon, HT. and Südhof, TC. 1995. J Biol Chem. 270: 2213-7. PMID: 7836452
- Exocytosis relating proteins in the nervous system. | Yamaguchi, K. and Akagawa, K. 1994. Neurosci Res. 20: 289-92. PMID: 7870381
- Synaptic vesicle membrane fusion complex: action of clostridial neurotoxins on assembly. | Hayashi, T., et al. 1994. EMBO J. 13: 5051-61. PMID: 7957071
- A role for synaptotagmin (p65) in regulated exocytosis. | Elferink, LA., et al. 1993. Cell. 72: 153-9. PMID: 8422678
- Stimulation of NSF ATPase activity by alpha-SNAP is required for SNARE complex disassembly and exocytosis. | Barnard, RJ., et al. 1997. J Cell Biol. 139: 875-83. PMID: 9362506
- Structural organization of the synaptic exocytosis core complex. | Lin, RC. and Scheller, RH. 1997. Neuron. 19: 1087-94. PMID: 9390521
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
NSF Antibody (A-3) | sc-515043 | 200 µg/ml | $316.00 | |||
NSF Antibody (A-3): m-IgG Fc BP-HRP Bundle | sc-531273 | 200 µg Ab; 10 µg BP | $354.00 | |||
NSF Antibody (A-3): m-IgGκ BP-HRP Bundle | sc-524915 | 200 µg Ab, 40 µg BP | $354.00 | |||
NSF Antibody (A-3): m-IgG2a BP-HRP Bundle | sc-547747 | 200 µg Ab; 10 µg BP | $354.00 | |||
NSF Antibody (A-3) AC | sc-515043 AC | 500 µg/ml, 25% agarose | $416.00 | |||
NSF Antibody (A-3) HRP | sc-515043 HRP | 200 µg/ml | $316.00 | |||
NSF Antibody (A-3) FITC | sc-515043 FITC | 200 µg/ml | $330.00 | |||
NSF Antibody (A-3) PE | sc-515043 PE | 200 µg/ml | $343.00 | |||
NSF Antibody (A-3) Alexa Fluor® 488 | sc-515043 AF488 | 200 µg/ml | $357.00 | |||
NSF Antibody (A-3) Alexa Fluor® 546 | sc-515043 AF546 | 200 µg/ml | $357.00 | |||
NSF Antibody (A-3) Alexa Fluor® 594 | sc-515043 AF594 | 200 µg/ml | $357.00 | |||
NSF Antibody (A-3) Alexa Fluor® 647 | sc-515043 AF647 | 200 µg/ml | $357.00 | |||
NSF Antibody (A-3) Alexa Fluor® 680 | sc-515043 AF680 | 200 µg/ml | $357.00 | |||
NSF Antibody (A-3) Alexa Fluor® 790 | sc-515043 AF790 | 200 µg/ml | $357.00 | |||
NSF (A-3) Neutralizing Peptide | sc-515043 P | 100 µg/0.5 ml | $68.00 |