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Date published: 2025-7-14
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EWS (C-9): sc-48404. Immunofluorescence detection of EWS in formalin-fixed HeLa cells showing nuclear localization. Detect
EWS (C-9): sc-48404. Immunofluorescence detection of EWS in formalin-fixed HeLa cells showing nuclear localization. Detection reagent used: m-IgGκ BP-FITC: sc-516140.
EWS (C-9): sc-48404. Immunofluorescence detection of EWS in formalin-fixed HeLa cells showing nuclear localization. Detection reagent used: m-IgGκ BP-FITC: sc-516140.

m-IgGκ BP-FITC: sc-516140

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Datasheets
  • mouse IgG kappa binding protein (m-IgGκ BP) conjugated to fluorescein (FITC)
  • supplied at 200 µg in 0.5 ml volume
  • Strongly recommended alternative to conventional anti-mouse IgG secondary antibodies for Immunofluorescence (IF) and Flow Cytometry (FCM)
  • Suitable for binding to most, but not all, mouse IgGκ light chain immunoglobulins; not suitable for use with mouse monoclonal IgGλ light chain antibodies
  • Highly specific reagent that provides strong signal with minimal background and virtually complete elimination of lot to lot variation associated with conventionally generated secondary antibodies
  • While we continue to offer conventional fluorescein (FITC) conjugates (525 nm emission) for immunofluorescent cell staining, we strongly recommend CruzFluor™ 488 Binding Protein (525 nm emission) as an alternative and much superior reagent.
  • Mouse IgG binding proteins are recommended for some, but not all of our monoclonal antibodies. Product descriptions on our monoclonal antibody product pages will state if the Mouse IgG binding protein is the preferred detection reagent for that product.

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Description
Datasheets and Protocols

Mouse IgGκ light chain binding protein is a strongly recommended alternative to conventional goat/rabbit anti-mouse IgG secondary antibodies for mmunofluorescence (IF) and Flow Cytometry (FCM) signal enhancement. Mouse IgGκ light chain binding protein is a highly specific reagent that provides strong signal with minimal background and virtually complete elimination of lot to lot variation associated with conventionally generated secondary antibodies. Mouse IgGκ light chain binding protein (m-IgGκ BP) conjugated to fluorescein isothiocyanate (FITC) is suitable for binding to mouse IgGκ light chain immunoglobulins, comprising a vast majority of mouse monoclonal primary antibodies; not suitable for use with mouse monoclonal IgGλ light chain primary antibodies.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

Ordering Information

Product NameCatalog #UNITPriceQtyFAVORITES

m-IgGκ BP-FITC

sc-516140
200 µg/0.5 ml
$92.00

Will this product (m-IgGκ BP-FITC: sc-516140), which is specific for kappa light chain, bind to your normal mouse IgG1 sc-3890. This normal mouse IgG1 does not specify kappa or lambda. Please clarify.

Asked by: DrDW
Thank you for your question. Yes, sc-516140 will work with sc-3890. Another alternative is sc-516606 : https://www.scbt.com/p/normal-mouse-igg-alexa-fluor-488
Answered by: BlakeJ
Date published: 2023-03-21

Can m-IgGκ BP-FITC (sc-516140) be used as a secondary staining for ACE2 Antibody (E-11) (sc-390851)?

Asked by: DrDW
This can be used a secondary for sc-390851
Answered by: Technical Service
Date published: 2023-03-13

I am planning to multicolor-immunostain cultured hippocampal neurons using sc-516140. Would sc-516140 cross-react the IgG kappa light chain of other species (e.g., goat, rabbit, guinea pig, chicken-IgY, etc) and cause non-specific signals?

Asked by: Nijima
Thank you for your question. It would be helpful if you could call us, allowing for a more interactive discussion of this and other related questions.
Answered by: Technical Support
Date published: 2021-07-23

What is the RRID for this antibody? Need this information in our paper. Thank you!

Asked by: Fang Wang
SCBT Is not responsible for the curation or assignment of RRID numbers for our products. This is an initiative from a consortium of researchers and The Antibody Registry. More information can be found on the website below. Http://antibodyregistry.org/
Answered by: Tech Service 1
Date published: 2021-02-04

This protein bind to IgGk. Does this mean the tertiary structure of IgG.

Asked by: yapyap
Thank you for your question. This binding protein is specific against the kappa light chain of IgG, IgA and IgM antibodies, which is a small polypeptide subunit of those immunoglobulins.
Answered by: Tech Support Europe
Date published: 2020-11-27

Can the these antibodies be labeled with m-IgIK BP-FITC: sc-52219, sc-52221, sc-52223, sc-57716, sc-71004? I didn't see kappa light chain in their descriptions.

Asked by: Christian_RTP
Thank you for your question. Yes, this secondary antibody can be used for detection with each of the primary antibodies listed. We confirmed these primary antibodies are all kappa light chain type. This information will be updated in their descriptions on our website.
Answered by: Technical Support
Date published: 2020-04-09

May i know this secondary antibody sc516140 is raised in goat or rabbit?thank you

Asked by: Fazlin
Thank you for your question. This item, sc-516140 is a mouse IgG kappa binding protein conjugated to FITC. It is not raised in goat or rabbit.
Answered by: Tech Service
Date published: 2019-10-23

Can m-IgGκ BP-FITC: sc-516140 be used for western blotting? and if yes how can bands be visualized

Asked by: Yahia
Thank you for your question. Yes, m-IgGκ BP-FITC: sc-516140, can be used for Western blotting as long as you have capabilities to visualize the results. FITC is a fluorochrome and you need to have the ability to see the resulting bands. We do not actually recommend sc-516140 for WB, so I would suggest using a CruzFluor conjugated secondary instead, which can be visualized using for example LiCor. You can find WB recommended secondaries here: https://www.scbt.com/browse/Secondary-Antibodies/_/N-1hoaxk4
Answered by: Tech Service
Date published: 2019-10-03
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Rated 5 out of 5 by from Great for flow cytometry or immunofluorescenceGreat detection reagent for flow cytometry and immunofluorescence. For use with primary mouse monoclonal antibodies that bind to mouse IgGκ Binding Protein. -SCBT QC
Date published: 2023-09-14
Rated 5 out of 5 by from sc-516140之前试用了很多家抗体，最终选择santa cruz公司的单克隆抗体。在做WB和免疫荧光实验，santa的单抗效果非常好。santa公司每年都有很多单抗小样供试用，非常棒。
Date published: 2022-08-13
Rated 4 out of 5 by from 免疫荧光早期申请过santa该产品试用装，用于免疫荧光，着色还可以，1：100-200稀释，性价比还是比较高的。
Date published: 2021-06-23
Rated 5 out of 5 by from Epigenome EditingNot long ago, I bought this product, which was shipped in time and well packaged.It's suitable for my experiment. it's very helpful to carry out the experiment.
Date published: 2021-06-03
Rated 4 out of 5 by from Secondary Antibody for flow cytometryThis antibody worked really well for flow cytometry
Date published: 2018-05-04
Rated 4 out of 5 by from Works fineI got this antibody as a free sample. It worked well for immunofluorescence applications but had some background.
Date published: 2017-12-01
Rated 5 out of 5 by from Works fine for cryosections!I used this antibody 1:80 for cryosections of mouse adrenals, with chromogranin A (sc-393941) 1:60 as primary. Works fine!
Date published: 2017-12-01
Rated 5 out of 5 by from 强烈推荐santa抗体这是我申请的试用装，染色效果很好，强烈推荐，货号SC-516140 SC-7273 ，进行流式检测，需要破核膜染色
Date published: 2017-11-28
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m-IgGκ BP-FITC is rated 4.6 out of 5 by 39.
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