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Synaptic activation of voltage-gated channels in the dendrites of hippocampal pyramidal neurons

Science. 1995 Apr 14;268(5208):301-4. doi: 10.1126/science.7716525.

Abstract

Activation of dendritic voltage-gated ion channels by local synaptic input was tested by simultaneous dendrite-attached patch-clamp recordings and whole-cell somatic voltage recordings made from CA1 pyramidal neurons in hippocampal slices. Schaffer collateral stimulation elicited subthreshold excitatory postsynaptic potentials (EPSPs) that opened voltage-gated sodium and calcium channels in the apical dendrites. The EPSP-activated sodium channels opened near the peak of the EPSP, whereas low voltage-activated calcium channels opened near the EPSP peak and during the decay phase. Dendritic high voltage-activated channels required somatic action potential generation or suprathreshold synaptic trains for activation. Dendritic voltage-gated channels are, therefore, likely to participate in dendritic integration of synaptic events.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Action Potentials
  • Animals
  • Calcium Channels / metabolism
  • Dendrites / physiology*
  • Ion Channel Gating*
  • Patch-Clamp Techniques
  • Pyramidal Cells / physiology*
  • Rats
  • Rats, Sprague-Dawley
  • Sodium Channels / metabolism
  • Synapses / physiology*
  • Synaptic Transmission
  • Tetrodotoxin / pharmacology

Substances

  • Calcium Channels
  • Sodium Channels
  • Tetrodotoxin