Extended Data Fig. 3: Purkinje cell responses to an airpuff stimulus in controls and CF-ChR2 animals with different expression levels.

a, b, Example electrophysiological traces and population histograms (N = 26 units from 4 mice) of Purkinje cell SSpks (grey) and CSpks (red) from control mice in response to an airpuff. c,d, Same as a,b but for low ChR2-CF expression levels (CF-ChR2-LE; N = 20 units from 4 mice). e,f, Same as c,d but for standard CF-ChR2 expression (N = 15 units from 5 mice). g, p(CSpk) to airpuff vs. spontaneous CSpk rate for each Purkinje cell of controls vs. mice with standard CF-ChR2 expression (CF-ChR2). h, Same as g, but comparing controls vs. mice with low CF-ChR2 expression (CF-ChR2-LE). i, Normalized cumulative histogram of timing of the first CSpk after airpuff onset (grey: controls N = 26 cells from 4 animals; light blue, CF-ChR2-LE, N = 20 cells from 4 animals; blue: CF-ChR2 N = 15 cells from 5 mice); controls vs CF-ChR2, P = 0.03*, KS-test; controls vs CF-ChR2-LE, P =  0.67n.s., KS-test. Shaded rectangle indicates time of airpuff (red). j, Average pause in SSpks after a CSpk: controls vs CF-ChR2, P = 0.15n.s., two-sample t-test; controls vs CF-ChR2-LE, P = 0.24n.s., two- sample t-test. k, Average CSpk doublets (2 CSpks occurring within 200 ms of each other) ratio to total number of CSpks during spontaneous and airpuff epochs; controls vs CF-ChR2, P = 0.14n.s., two-sample t-test (15 cells in 5 mice); controls vs CF-ChR2-LE, P = 0.24n.s., two-sample t-test (20 cells in 4 mice). l,m, Average number of airpuff-driven and spontaneous CSpk spikelets, respectively. (All two- sample t-test) Airpuff: controls vs CF-ChR2, P = 0.5n.s., 15 cells in 5 mice; controls vs CF-ChR2- LE, P = 0.3n.s., 20 cells in 4 mice. Spont.: controls vs CF-ChR2, P = 0.1n.s. 15 cells in 5 mice; controls vs CF-ChR2-LE, P = 0.1n.s., 20 cells in 4 mice.