Extended Data Fig. 7: Effect of BL-8040 plus pembrolizumab combination on the composition of immune cells in the tumor microenvironment.

a, Treatment promoted an increase in the density of CD3 + and CD8 + cells in the TME of pancreatic cancer patients. Tumor biopsies were harvested from subjects at pre- and on-treatment timepoints and cell density of CD3 + T cells and CD8 + T cells were calculated. Cell enumerations were done following IHC by manual counting and Image Analysis on paired biopsies of 10 subjects for CD3 + (BL-8040 Monotherapy Day 5, N = 5, red circles; BL-8040 plus pembrolizumab End of Cycle 2, N = 5, black circles) and 9 subjects for CD8 + (BL-8040 Monotherapy Day 5, N = 5, blue circles; BL-8040 plus pembrolizumab End of Cycle 2, N = 4, black circles). b, Treatment promoted an increase in the density of CXCR4 + T cells, CD4 + and activated CD8 + T cells (CD3 + CD8 + Granzyme B + ) and a decrease in the density of G-MDSCs (CD11B + CD33 + HLADR-CD15 + ) in the TME of pancreatic cancer patients. Density of different cell subtypes was assessed on screening and on-treatment biopsies; data presented as cell density compared to screening. Tumor infiltrating lymphocyte and MDSC markers were analyzed by Multi-Omyx on four evaluable paired biopsies (BL-8040 Monotherapy Day 5, N = 3, colored circles; BL-8040 plus pembrolizumab End of Cycle 2, N = 1, black circles).