Extended Data Figure 4: R-loops and RNAi promote the H3K9me2 mark over mouse β-actin terminator.
From: R-loops induce repressive chromatin marks over mammalian gene terminators
a, DIP performed on mouse β-actin gene in MEFs. b, RT–qPCR of total RNA from MEF cells on β-actin gene to detect antisense transcripts with region-specific forward primers. Average RT–qPCR values are ±s.d. from four biological repeats. c, Ago1 ChIP performed on mouse β-actin gene in MEFs. ChIP signal is normalized to intron 1 signal. d, Left, ratio of H3K9me2 ChIP signal versus H3 on mouse β-actin in MEFs. Middle, normalized H3K9me3 to total H3 levels. Right, ratio of H3K9me2 and H3K9me3 signal versus H3 signal on major satellites in MEFs. e, Ago1 ChIP in wild-type (grey bars) and Ago2-knockout (KO) (white bars) cells. Ago1 recruitment over mouse β-actin is enhanced upon Ago2 depletion. f, Left, ratio of H3K9me2 ChIP signal versus total H3 on β-actin gene in wild-type and G9a/Glp double-knockout mouse embryonic stem cells. Right, H3K9me2/H3 ratio on the mouse major satellites in wild-type and G9a/Glp double-knockout cells. Average ChIP and DIP values are ±s.d. from three biological repeats.