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Search Results (614)

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16 pages, 2037 KiB  
Article
Modulation of the Gut–Lung Axis by Water Kefir and Kefiran and Their Impact on Toll-like Receptor 3-Mediated Respiratory Immunity
by Stefania Dentice Maidana, Julio Nicolás Argañaraz Aybar, Leonardo Albarracin, Yoshiya Imamura, Luciano Arellano-Arriagada, Fu Namai, Yoshihito Suda, Keita Nishiyama, Julio Villena and Haruki Kitazawa
Biomolecules 2024, 14(11), 1457; https://doi.org/10.3390/biom14111457 - 17 Nov 2024
Viewed by 485
Abstract
The beneficial effect of milk kefir on respiratory heath has been previously demonstrated; however, water kefir and kefiran in the context of respiratory viral infections have not been investigated. Water kefir and kefiran could be alternatives to milk kefir for their application in [...] Read more.
The beneficial effect of milk kefir on respiratory heath has been previously demonstrated; however, water kefir and kefiran in the context of respiratory viral infections have not been investigated. Water kefir and kefiran could be alternatives to milk kefir for their application in persons with lactose intolerance or milk allergy and could be incorporated into vegan diets. Using mice models, this work demonstrated that the oral administration of water kefir or kefiran can modulate the respiratory Toll-like receptor (TLR3)-mediated innate antiviral immunity and improve the resistance to respiratory syncytial virus (RSV) infection. The treatment of mice with water kefir or kefiran for 6 days improved the production of interferons (IFN-β and IFN-γ) and antiviral factors (Mx2, OAS1, RNAseL, and IFITM3) in the respiratory tract after the activation of the TLR3 signaling pathway, differentially modulated the balance of pro- and anti-inflammatory cytokines, reduced RSV replication, and diminished lung tissue damage. Maintaining a proper balance between anti-inflammatory and pro-inflammatory mediators is vital for ensuring an effective and safe antiviral immune response, and the results of this work show that water kefir and kefiran would help to maintain that balance promoting a controlled inflammatory response that defends against infection while minimizing tissue damage. Full article
(This article belongs to the Section Natural and Bio-derived Molecules)
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<p>Effect of water kefir and kefiran on TLR3-mediated lung damage. Mice were fed water kefir or kefiran for 6 days and stimulated on days 7, 8, and 9 with the TLR3 agonist poly(I:C) by the nasal route. Mice without water kefir or kefiran treatment and stimulated with poly(I:C) were used as control. The concentrations of broncho-alveolar lavage (BAL) proteins and albumin, the activity of BAL lactate dehydrogenase (LDH), and lung histology were determined 2 days after TLR3 activation. Hematoxylin–eosin stain of histological slices of lung micrographs at 10× are shown. The results are expressed as mean ± SD. Significant differences were shown compared to the poly(I:C)-treated control group at <span class="html-italic">p</span> &lt; 0.05 (*) or <span class="html-italic">p</span> &lt; 0.01 (**).</p>
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<p>Effect of water kefir and kefiran on TLR3-mediated lung inflammatory cells infiltration. Mice were fed water kefir or kefiran for 6 days and stimulated on days 7, 8 and 9 with the TLR3 agonist poly(I:C) by the nasal route. Mice without water kefir or kefiran treatment and stimulated with poly(I:C) were used as control. The numbers of broncho-alveolar lavage (BAL) leukocytes, macrophages, neutrophils, and lymphocytes were determined 2 days after TLR3 activation. The results are expressed as mean ± SD. Significant differences were shown compared to the poly(I:C)-treated control group at <span class="html-italic">p</span> &lt; 0.05 (*) or <span class="html-italic">p</span> &lt; 0.01 (**).</p>
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<p>Effect of water kefir and kefiran on TLR3-mediated lung cytokine response. Mice were fed water kefir or kefiran for 6 days and stimulated on days 7, 8, and 9 with the TLR3 agonist poly(I:C) by the nasal route. Mice without water kefir or kefiran treatment and stimulated with poly(I:C) were used as control. The concentrations of broncho-alveolar lavage (BAL) TNF-α, IL-6, KC, MCP-1, IFN-β, IFN-γ, IL-10, and IL-27 were determined 2 days after TLR3 activation. The results are expressed as mean ± SD. Significant differences were shown compared to the poly(I:C)-treated control group at <span class="html-italic">p</span> &lt; 0.05 (*) or <span class="html-italic">p</span> &lt; 0.01 (**).</p>
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<p>Effect of water kefir and kefiran on TLR3-mediated alveolar macrophages antiviral factors response. Mice were fed water kefir or kefiran for 6 days; on day 7, alveolar macrophages were collected and stimulated in vitro with the TLR3 agonist poly(I:C). Alveolar macrophages obtained from mice without water kefir or kefiran treatment and stimulated in vitro with poly(I:C) were used as control. The expressions of IFN-β, IFN-γ, Mx2, OAS1, RNAseL, and IFITM3 were determined 12 h after TLR3 activation. The results are expressed as mean ± SD. Significant differences were shown compared to the poly(I:C)-treated control alveolar macrophages at <span class="html-italic">p</span> &lt; 0.05 (*) or <span class="html-italic">p</span> &lt; 0.01 (**).</p>
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<p>Effect of water kefir and kefiran on the resistance to RSV infection. Mice were fed water kefir or kefiran for 6 days and challenged on day 7 with RSV by the nasal route. Mice without water kefir or kefiran treatment and infected with RSV were used as control. The RSV lung titers, the concentrations of broncho-alveolar lavage (BAL) proteins and albumin, and the activity of BAL lactate dehydrogenase (LDH) were determined 2 days after RSV challenge. The results are expressed as mean ± SD. Significant differences were shown compared to the RSV-infected control group at <span class="html-italic">p</span> &lt; 0.05 (*) or <span class="html-italic">p</span> &lt; 0.01 (**).</p>
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11 pages, 254 KiB  
Review
Plant-Based Diets in Pediatric Subjects: Heart-Healthy Option or Dangerous Choice?
by Maria Elena Capra, Delia Monopoli, Brigida Stanyevic, Antonella Giudice, Nicola Mattia Decarolis, Susanna Esposito and Giacomo Biasucci
Healthcare 2024, 12(22), 2290; https://doi.org/10.3390/healthcare12222290 - 16 Nov 2024
Viewed by 343
Abstract
Background/Objectives: Plant-based diets (PBDs) are dietary patterns characterized by a certain degree of animal-derived food exclusion. PBDs can be divided into different dietary patterns, from vegetarian to vegan, depending on the degree and the extent of animal-derived food avoidance. PBDs are becoming epidemically [...] Read more.
Background/Objectives: Plant-based diets (PBDs) are dietary patterns characterized by a certain degree of animal-derived food exclusion. PBDs can be divided into different dietary patterns, from vegetarian to vegan, depending on the degree and the extent of animal-derived food avoidance. PBDs are becoming epidemically popular among the general population, including adult subjects as well as children and adolescents, who often follow the dietary pattern chosen by their families. Methods: Our narrative review aims to analyze the most frequently adopted plant-based dietary patterns in children and adolescents and to evaluate their feasibility, advantages, and risks in terms of health promotion and disease prevention in the developmental age. The MEDLINE–PubMed database was searched to collect and select publications from 1980 to 2024. Results: Subjects following these dietary patterns, especially vegan diets, must be under strict nutritional control and receive adequate micronutrients and vitamin supplementation. Conclusions: Nutrition-skilled professionals should be adequately updated and informed about the feasibility and the risks of these different patterns’ adoption at different ages, as they should guide and accompany children and adolescents and their families in their nutritional choices without prejudices, granting adequate macronutrient and micronutrient intake, adequate growth and neurodevelopment. Full article
15 pages, 2771 KiB  
Article
Sustainable Omega-3 Lipid Production from Agro-Industrial By-Products Using Thraustochytrids: Enabling Process Development, Optimization, and Scale-Up
by Guilherme Anacleto dos Reis, Brigitte Sthepani Orozco Colonia, Walter Jose Martínez-Burgos, Diego Ocán-Torres, Cristine Rodrigues, Gilberto Vinícius de Melo Pereira and Carlos Ricardo Soccol
Foods 2024, 13(22), 3646; https://doi.org/10.3390/foods13223646 - 16 Nov 2024
Viewed by 497
Abstract
Thraustochytrids are emerging as a valuable biomass source for high-quality omega-3 polyunsaturated fatty acids (PUFAs), crucial for both human and animal nutrition. This research focuses on cultivating Schizochytrium limacinum SR21 using cost-effective agro-industrial by-products, namely sugarcane molasses (SCM), corn steep liquor (CSL), and [...] Read more.
Thraustochytrids are emerging as a valuable biomass source for high-quality omega-3 polyunsaturated fatty acids (PUFAs), crucial for both human and animal nutrition. This research focuses on cultivating Schizochytrium limacinum SR21 using cost-effective agro-industrial by-products, namely sugarcane molasses (SCM), corn steep liquor (CSL), and residual yeast cream (RYC), to optimize biomass and lipid production through a comprehensive multistep bioprocess. The study involved optimization experiments in shake flasks and stirred-tank bioreactors, where we evaluated biomass, lipid content, and DHA yields. Shake flask optimization resulted in significant enhancements in biomass, lipid content, and lipid production by factors of 1.12, 1.72, and 1.92, respectively. In a 10 L stirred-tank bioreactor, biomass surged to 39.29 g/L, lipid concentration increased to 14.98 g/L, and DHA levels reached an impressive 32.83%. The optimal concentrations identified were 66 g/L of SCM, 24.5 g/L of CSL, and 6 g/L of RYC, achieving a desirability index of 0.87, aimed at maximizing biomass and lipid production. This study shows that agro-industrial by-products can be effective and low-cost substrates for producing lipids using thraustochytrids, offering a sustainable option for omega-3 PUFA production. The findings support future improvements in bioprocesses and potential uses of thraustochytrid biomass in food fortification, dietary supplements, nutraceuticals, and as vegan omega-3 sources. Full article
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<p>The Pareto chart shows standardized effects and compares predicted vs. observed values in the central composite design (CCD) for biomass and lipid production, using a 5% confidence level (blue dotted line) and prediction level (red dotted line).</p>
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<p>Prediction profiler and desirability for optimization of multiple responses Y1: Biomass (g/L), Y2: Lipid content (%), and Y3: Lipids (g/L).</p>
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<p>Response surfaces from CCD 23 regression models optimize bioprocess using sugarcane molasses (66 g/L), corn steep liquor (24.5 g/L), and residual yeast cream (6 g/L) based on predictions and desirability profiles.</p>
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<p>Batch kinetic curves of a bioprocess using thraustochytrid and agro-industrial by-products as substrate include: (<b>A</b>) Scale-up in a stirred-tank bioreactor (STBR). (<b>B</b>) 250 mL shake flask. (<b>C</b>) Logarithmic cell growth (Ln X). (<b>D</b>) Substrate consumption rate (Qs) and product formation rate (Qp). (<b>E</b>) Yield evolution curves for changes in biomass (ΔX), product (ΔP), and substrate (ΔS) in a 10 L STBR. (<b>F</b>) Yield evolution curves for changes in the same parameters in a 250 mL shake flask. The standard deviations depicted in <a href="#foods-13-03646-f004" class="html-fig">Figure 4</a>B were so minute that they were undetectable within the confines of the image.</p>
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<p>The objective of this study is to employ gas chromatography spectroscopy to identify the specific fatty acids present in the biomass. As can be observed, the four most prominent peaks represent pentadecanoic (11.54%), hexadecanoic (34.28%), heptadecanoic (7.34%), and docosahexaenoic (36.65%) fatty acids.</p>
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35 pages, 8115 KiB  
Article
Fashion Wears Sustainable Leather: A Social and Strategic Analysis Toward Sustainable Production and Consumption Goals
by Idiano D’Adamo, Marta Gagliarducci, Martina Iannilli and Valeria Mangani
Sustainability 2024, 16(22), 9971; https://doi.org/10.3390/su16229971 - 15 Nov 2024
Viewed by 591
Abstract
While fashion and sustainability are often proposed as opposing concepts, many companies in the fashion industry are actively working to transform their operations to achieve greater sustainability. The production of leather goods significantly contributes to air and water pollution and generates challenging waste [...] Read more.
While fashion and sustainability are often proposed as opposing concepts, many companies in the fashion industry are actively working to transform their operations to achieve greater sustainability. The production of leather goods significantly contributes to air and water pollution and generates challenging waste disposal issues. The present study explored emerging sustainable paradigms in the leather goods industry through both social and strategic analyses. The results indicate that, although consumers are often unaware of the differences among leather types, there is a growing openness to sustainable alternatives and a willingness to pay for these products. Quality and durability are key consumer priorities, though a bias toward fast fashion persists. Notably, while consumers value vegan leather as a sustainable alternative, experts caution that synthetic leather alternatives may have greater environmental drawbacks compared to traditional leather. The work supports SDG 12 by advocating for reduced reliance on materials with a high environmental impact and the adoption of sustainable alternatives. Enhanced consumer communication may also drive the industry toward a model that integrates environmental and social responsibility with profitability. Given the prestigious status of leather goods within the Made in Italy initiative, the present work suggests that the value of vegan leather may increase within the global market. Full article
(This article belongs to the Section Development Goals towards Sustainability)
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<p>Scientific production by nation (circular fashion and circular premium).</p>
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<p>Word cloud (circular fashion and circular premium).</p>
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<p>Co-occurrence network (circular fashion and circular premium).</p>
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<p>Thematic map (circular fashion and circular premium).</p>
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<p>Scientific production by nation (leather theme).</p>
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<p>Word cloud (leather theme).</p>
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<p>Co-occurrence network (leather theme).</p>
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<p>Thematic map (leather theme)<b>.</b></p>
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<p>PRISMA diagram.</p>
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<p>Sustainable behaviors in the apparel industry.</p>
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<p>Attributes considered in the purchase of traditional leather.</p>
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<p>Attributes considered in the purchase of vegan leather.</p>
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<p>WTP for leather bags from different sources.</p>
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<p>WTP for leather shoes from different sources.</p>
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<p>WTP for bags made from vegan and vegetable leather.</p>
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<p>WTP for shoes made from vegan and vegetable leather.</p>
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<p>Interest in trying bags and shoes made from alternative materials.</p>
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<p>Annual scientific production (circular fashion and circular premium).</p>
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<p>Geographical distribution matching authors (circular fashion and circular premium).</p>
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<p>Annual scientific production (leather theme).</p>
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<p>Geographical distribution of matching authors (leather theme). Light blue = single country publication and light red = multiple countries publication.</p>
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24 pages, 1264 KiB  
Review
Spirulina—An Invaluable Source of Macro- and Micronutrients with Broad Biological Activity and Application Potential
by Izabela Podgórska-Kryszczuk
Molecules 2024, 29(22), 5387; https://doi.org/10.3390/molecules29225387 - 15 Nov 2024
Viewed by 505
Abstract
With population growth expected in the near future and the planet’s limited resources, alternative food sources are already being looked for. In this context, spirulina is called the food of the future due to its rich nutritional composition. This blue–green alga is primarily [...] Read more.
With population growth expected in the near future and the planet’s limited resources, alternative food sources are already being looked for. In this context, spirulina is called the food of the future due to its rich nutritional composition. This blue–green alga is primarily a valuable source of protein (55–70%) containing all essential amino acids. In its composition, it also contains unsaturated fatty acids, minerals, vitamins, and pigments, including the valuable protein–pigment complex—phycocyanin. Due to its high content of complete protein and minerals such as iron and calcium, it is an excellent addition to diets, especially those of vegans and vegetarians. Despite several limitations to the use of spirulina, including its distinctive marine flavour, low consumer awareness, or relatively high price, scientists are attempting to enrich many food products with the microalga. This is supported not only by the improved nutritional composition of the fortified product but also by spirulina’s impact on sustainable food production. Therefore, this review aims to create consumer attention by presenting spirulina as a valuable and sustainable food source with health-promoting potential and great future significance. Full article
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<p>Chemical structure of phycocyanin from <span class="html-italic">A. platensis</span> [<a href="#B7-molecules-29-05387" class="html-bibr">7</a>].</p>
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<p>Comparison of spirulina production in open, closed, and hybrid systems [<a href="#B41-molecules-29-05387" class="html-bibr">41</a>,<a href="#B54-molecules-29-05387" class="html-bibr">54</a>].</p>
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9 pages, 1124 KiB  
Article
An Assessment of the Strength and Physical Properties of Edible Tableware from Flax Seed and Flaxseed Cake
by Dariusz Andrejko and Agata Blicharz-Kania
Materials 2024, 17(22), 5510; https://doi.org/10.3390/ma17225510 - 12 Nov 2024
Viewed by 288
Abstract
Alternatives to traditional disposable plastic tableware are constantly sought. The aim of the study was to assess the possibility of using oilseeds and their press cakes for the production of edible tableware. Edible vegan plates (P) and bowls (B) were produced. The basic [...] Read more.
Alternatives to traditional disposable plastic tableware are constantly sought. The aim of the study was to assess the possibility of using oilseeds and their press cakes for the production of edible tableware. Edible vegan plates (P) and bowls (B) were produced. The basic ingredients used for production were flax seeds (S) or flax press cake (C). Plates made using press cakes under a pressure of 3 kg deformed to a lesser extent than those containing seeds. However, they were more susceptible to crumbling during shaking. The colour of the tableware made on the basis of flax press cakes was lighter and was characterised by a higher chromaticity in the yellow and red direction. Significantly higher water absorption was characteristic of the tableware in which flax press cakes were used instead of flax seeds. The lowest water absorption (17.14%) after 30 min of soaking was recorded for the PS sample. After the test simulating the use of the tableware, a significant reduction in strength was observed overall (except for the PS test). The panelists rated the consistency and palatability of the PS, BS and PC tests very similarly. The highest overall acceptability was noted for the BS and PC tests. In conclusion, the development of edible bowls and plates made from flax seeds or flaxseed cake is an alternative solution for the production of environmentally friendly tableware. Full article
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<p>Appearance of the obtained edible tableware. PS—flaxseed plates, BS—flaxseed bowl, PC—flaxseed cake plates and BC—flaxseed cake bowl.</p>
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<p>Water absorption of edible tableware. PS—flaxseed plates, BS—flaxseed bowl, PC—flaxseed cake plates and BC—flaxseed cake bowl. Different letters indicate significantly different values at a given point (Tukey test. <span class="html-italic">p</span> ≤ 0.05).</p>
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<p>Sensory evaluation of edible tableware. PS—flaxseed plates, BS—flaxseed bowl, PC—flaxseed cake plates and BC—flaxseed cake bowl.</p>
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14 pages, 2916 KiB  
Article
What Is the Relationship Between Sensory Attributes Identified Using CATA (Check-All-That-Apply) Questionnaire and Consumer Acceptance of Cookies Using Plant-Based Oils?
by Juyoun Lee and Seyoung Ju
Foods 2024, 13(22), 3593; https://doi.org/10.3390/foods13223593 - 10 Nov 2024
Viewed by 485
Abstract
The purpose of this study was to investigate sensory attributes identified using a CATA questionnaire and consumer acceptance of cookies using plant-based oils and to determine which sensory attributes influence and improve purchase intention of cookies using plant-based oils. Frequency analysis, one-way analysis [...] Read more.
The purpose of this study was to investigate sensory attributes identified using a CATA questionnaire and consumer acceptance of cookies using plant-based oils and to determine which sensory attributes influence and improve purchase intention of cookies using plant-based oils. Frequency analysis, one-way analysis of variance, correspondence analysis, Pearson correlation analysis, and multiple factor analysis were used for statistical evaluation. Six cookie samples, five with types of vegetable oil (canola, brown rice, coconut, soybean, olive) and one with butter, were evaluated by 112 consumers. Of the 28 sensory attributes, the 11 sensory terms of milk, savory, sweet aroma/odor, sweet, roasted grain, milk taste/flavor, hardness, “crispy”, “stuffy”, roughness, and “crumble” were significantly different among samples (p < 0.001). In the consumer acceptance and purchase intention tests, participants evaluated 12 attributes, namely, overall liking, appearance, color, aroma/smell, overall taste, savory flavor, flavor, sweet taste, aftertaste, texture, “smooth”, “crispy”, “try again”, recommendations, and familiarity, on a 9-point structured hedonic scale. All attributes except “smooth” were significantly different among the six cookie samples tested (p < 0.001). Samples with coconut oil and butter had the highest consumer acceptance. In particular, in a correspondence analysis, the samples with butter and coconut oil positioned in the positive direction (+) of Dimension (Component) 1 were closely related with mouthfeel, sweet aroma, sweet taste, “crispy”, “try again”, recommendation, familiarity, and overall liking. Our results suggest that cookies with coconut oil could be a substitute for animal-fat-based cookies with butter. We hope that our research will provide fundamental data for the development of vegan bakery products that meet the needs of consumers and food companies. Full article
(This article belongs to the Section Plant Foods)
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<p>Correspondence analysis (CA) biplot of six cookie samples using 28 sensory attributes identified using CATA. Dim is an abbreviation for dimension.</p>
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<p>Pearson correlation coefficient heatmap regarding the consumer acceptance of cookie samples.</p>
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<p>Results of Multiple Factor Analysis (MFA) by sensory characteristics, consumer acceptance, and purchase attention of six cookie samples; plots for samples (<b>a</b>) and sensory characteristics identified using CATA, consumer acceptance, and purchase intention (<b>b</b>).</p>
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16 pages, 1771 KiB  
Review
The Influence of a Plant-Based Diet on Skin Health: Inflammatory Skin Diseases, Skin Healing, and Plant-Based Sources of Micro- and Macro-Nutrients
by Mildred Min, Anurag Tarmaster, Apple Bodemer and Raja K. Sivamani
Life 2024, 14(11), 1439; https://doi.org/10.3390/life14111439 - 7 Nov 2024
Viewed by 832
Abstract
Dietary patterns have been shown to worsen or alleviate several dermatological diseases. A well-balanced, plant-based diet is known to have anti-inflammatory, probiotic, and antioxidant properties, along with weight loss-promoting effects. Moreover, a plant-based diet has a low glycemic load, improving metabolic disease. Due [...] Read more.
Dietary patterns have been shown to worsen or alleviate several dermatological diseases. A well-balanced, plant-based diet is known to have anti-inflammatory, probiotic, and antioxidant properties, along with weight loss-promoting effects. Moreover, a plant-based diet has a low glycemic load, improving metabolic disease. Due to these qualities, plant-based diets may have beneficial effects on inflammatory skin conditions. In this review, we aim to discuss the possible mechanisms by which a plant-based diet reduces disease severity in psoriasis, acne, hidradenitis suppurativa, and atopic dermatitis. We also aim to clarify how a plant-based diet may influence skin healing and identify sources of vitamins, nutrients, fatty acids, and protein in a well-balanced, plant-based diet. We performed a literature search on PubMed/MEDLINE databases with the following keywords: “plant-based” OR “vegan” OR “vegetarian” OR “meat” OR “diet” AND “psoriasis” OR “hidradenitis suppurativa” OR “acne” OR “atopic dermatitis” OR “skin healing” OR “dermatology”. Our findings demonstrate that plant-based foods may improve inflammatory skin diseases by supporting the gut microbiome, exerting anti-inflammatory effects, providing barrier support, and improving glycemic control. With the proper education, there is an abundance of plant-based food sources or supplements that contain riboflavin, vitamin B12, vitamin A, omega-3 fatty acids, and protein, thereby ameliorating the risk of nutritional deficiencies. Thus, a plant-based diet may have therapeutic potential in dermatology. In spite of the evidence available, there is a paucity of clinical studies focusing specifically on plant-based diets and dermatologic conditions and further investigation is warranted. Full article
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<p><b>PRISMA flow diagram of selected studies.</b> This figure was created in Microsoft Word version 16.90 (Redmond, WA, USA).</p>
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<p><b>Proposed mechanisms of a plant-based diet on inflammatory skin diseases.</b> Plant-based foods are rich in fiber and probiotics which support bacteria that metabolize and produce SCFAs that are key regulators of gut health. Plant-based diets contain vitamin K, eliminate dairy sources, promote weight loss, and improve ratios of omega-3 fatty acids to omega-6 fatty acids, thereby reducing systemic inflammation. Plant-based foods also contain bioactive compounds such as carotenoids, isoflavones, and antioxidants that act on skin barrier support and function. Altogether, these mechanisms lead to a healthier gut microbiome, reduced systemic inflammation, and healthy skin. This figure was created on Canva (Sydney, Australia). Abbreviations: SCFA, short-chain fatty acid.</p>
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<p>Omega-6 and omega-3 pathways demonstrating shared enzymes and food sources of LA, ALA, AA, EPA, and DHA. When the ratio of omega-6 to omega-3 is high (&gt;4:1), enzymes are used up in the LA pathway, which results in a proinflammatory state. This figure was created on Canva (Sydney, Australia).</p>
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13 pages, 3495 KiB  
Article
Enhancement of Skin Anti-Wrinkling Effects of Arthrospira maxima Phycocynobilin by Combining with Wheat Bran Extract
by Eun-Jeong Koh, Taeho Kim, Yong-Kyun Ryu, Won-Kyu Lee, In-Yung Sunwoo, Hyang Seon Ro, Gibeom Jeon, Gyu Rae Kim, Hyeon Yong Lee and Woon-Yong Choi
Appl. Sci. 2024, 14(22), 10216; https://doi.org/10.3390/app142210216 - 7 Nov 2024
Viewed by 361
Abstract
Despite the many beneficial effects of phycocyanobilin (PCB) on human skin, its cosmetic applications have not been extensively investigated owing to its light and temperature sensitivity. This is the first report of PCB extract (SP) derived from marine Arthrospira maxima having skin anti-wrinkling [...] Read more.
Despite the many beneficial effects of phycocyanobilin (PCB) on human skin, its cosmetic applications have not been extensively investigated owing to its light and temperature sensitivity. This is the first report of PCB extract (SP) derived from marine Arthrospira maxima having skin anti-wrinkling effects associated with antioxidant efficacy and reduction of intracellular reactive oxygen species (ROS) production. We obtained 46.63 ± 1.72 mg PCB/g dry weight of A. maxima in SP through an ethanol extraction process. PCB extracts showed strong effects in increasing collagen synthesis and decreasing matrix metalloproteinases (MMP-1) production. Interestingly, skin anti-wrinkling effects of the PCB extracts were significantly increased by the addition of wheat bran extracts (WB), up to 20–30% of the effects of PCB at all concentrations, possibly due to the synergistic effects of soluble globulins and other active substances in WB. Moreover, the mixture of SP and WB (SPWB) greatly reduced cell cytotoxicity to approximately 15% of that of PCB. SPWB upregulated and downregulated the expression of collagen type I α1 (Col1A1) and MMP-1, respectively, although the downregulation of MMP-1 was higher than that of Col1A1. The optimal SPWB concentration for maintaining the highest skin anti-wrinkling effects was 0.5 mg/mL. We show that SPWB holds promise as a vegan cosmaceutical. Full article
(This article belongs to the Section Applied Biosciences and Bioengineering)
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<p>PCB structure and comparison of HPLC chromatogram of standard PCB and SP. (<b>a</b>) PCB structure (<b>b</b>) HPLC chromatogram of standard PCB and SP. Blue line: PCB standard; Black line: SP, the PCB extract from <span class="html-italic">Arthrospira maxima</span>, prepared by water extraction using ultrasonication at room temperature followed by ethanolic extraction at 70 °C.</p>
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<p>Cytotoxicity of the extracts against human skin fibroblasts. PCB, PCB standard; SP, the PCB extract from <span class="html-italic">Arthrospira maxima</span>, prepared by water extraction using ultrasonication at room temperature followed by ethanolic extraction at 70 °C; WB, the wheat bran extract prepared by water extraction at 4 °C for eight hours; SPWB, a mixture of SP and WB containing the same amounts of PCB and SP. Cytotoxicity of PCB standard (<b>a</b>) SP (<b>b</b>), WB (<b>c</b>), and SPWB (<b>d</b>). The mean ± SD values for triplicate experiments are shown; error bars represent the SD. * <span class="html-italic">p</span> &lt; 0.05, ** <span class="html-italic">p</span> &lt; 0.01, and *** <span class="html-italic">p</span> &lt; 0.001 compared with the non-treated group.</p>
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<p>DPPH free radical-scavenging activity of the extracts. Human fibroblasts were subjected to different treatments, as indicated. PCB, PCB standard; SP, the PCB extract from <span class="html-italic">Arthrospira maxima</span>, prepared by water extraction using ultrasonication at room temperature followed by ethanolic extraction at 70 °C; WB, the wheat bran extract prepared by water extraction at 4 °C for eight hours; SPWB, a mixture of SP and WB containing the same amounts of PCB and SP. Trolox (50 µg/mL) was used as a positive control. The mean ± SD values for triplicate experiments are shown; error bars represent the SD. *** <span class="html-italic">p</span> &lt; 0.001 compared with the Trolox group.</p>
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<p>Comparison of intracellular ROS production in UVB-irradiated human skin fibroblasts treated with the different extracts. PCB, PCB standard; SP, the PCB extract from <span class="html-italic">Arthrospira maxima</span>, prepared by water extraction using ultrasonication at room temperature followed by ethanolic extraction at 70 °C; WB, the wheat bran extract prepared by water extraction at 4 °C for eight hours; SPWB, a mixture of SP and WB containing the same amounts of PCB and SP. The mean ± SD values for triplicate experiments are shown; error bars represent the SD. * <span class="html-italic">p</span> &lt; 0.05, ** <span class="html-italic">p</span> &lt; 0.01 and *** <span class="html-italic">p</span> &lt; 0.001 compared with the control (UV+) group.</p>
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<p>Collagen levels in UVB-irradiated human skin fibroblasts subjected to different treatments. PCB, PCB standard; SP, the PCB extract from <span class="html-italic">Arthrospira maxima</span>, prepared by water extraction using ultrasonication at room temperature followed by ethanolic extraction at 70 °C; WB, the wheat bran extract prepared by water extraction at 4 °C for eight hours; SPWB, a mixture of SP and WB containing the same amounts of PCB and SP. TGF-β1 (0.1 ng/mL) was used as a positive control. The mean ± SD values for triplicate experiments are shown; error bars represent the SD. *** <span class="html-italic">p</span> &lt; 0.001 compared with the control (UV+) group.</p>
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<p>Inhibition of MMP-1 expression in UVB-irradiated human skin fibroblasts subjected to different treatments. PCB, PCB standard; SP, the PCB extract from <span class="html-italic">Arthrospira maxima</span>, prepared by water extraction using ultrasonication at room temperature followed by ethanolic extraction at 70 °C; WB, the wheat bran extract prepared by water extraction at 4 °C for eight hours; SPWB, a mixture of SP and WB containing the same amounts of PCB and SP. Retinol (0.1% <span class="html-italic">w/v</span>) was used as a positive control. The mean ± SD values for separate triplicate experiments are shown; error bars represent the SD. *** <span class="html-italic">p</span> &lt; 0.001 compared with the control (UV+) group.</p>
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<p>Up- and downregulation of mRNA levels of collagen (Col1A1) and MMP-1, respectively (<b>a</b>) in UVB-irradiated human skin fibroblasts, CCD-986sk. The relative densitometric intensities (<b>b</b>,<b>c</b>) of the bands, normalized against the mRNA levels of the housekeeping gene, GAPDH, in the different treatments, are shown. PCB, PCB standard; SP: the PCB extract from <span class="html-italic">Arthrospira maxima</span>, prepared by water extraction using ultrasonication at room temperature followed by ethanolic extraction at 70 °C; WB: the wheat bran extract prepared by water extraction at 4 °C for eight hours; SPWB: a mixture of SP and WB containing the same amounts of PCB and SP. TGF-β1 (0.1 ng/mL) was used as a positive control. The mean ± SD values for triplicate experiments are shown; error bars indicate the SD. ** <span class="html-italic">p</span> &lt; 0.01, *** <span class="html-italic">p</span> &lt; 0.001 compared with the control (UV+) group.</p>
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25 pages, 453 KiB  
Review
How to Keep Lactose Avoiders Healthy
by Zlatina Chengolova, Petar Shentov, Radina Ivanova and Reni Syarova
Dairy 2024, 5(4), 702-726; https://doi.org/10.3390/dairy5040052 - 6 Nov 2024
Viewed by 535
Abstract
A large portion of the world’s population has lactose intolerance. Fundamentally, this condition occurs when the small intestine does not produce enough of the lactase enzyme, which digests the disaccharide lactose in milk. Lactose avoiders might unconsciously decide to limit or exclude milk [...] Read more.
A large portion of the world’s population has lactose intolerance. Fundamentally, this condition occurs when the small intestine does not produce enough of the lactase enzyme, which digests the disaccharide lactose in milk. Lactose avoiders might unconsciously decide to limit or exclude milk and dairy products from their diets. This group includes people with lactose intolerance, people with an allergy to milk protein, vegans, and those expressing personal preferences. Lactose avoiders are often self-reported as being milk intolerant. In this review, specific amounts of lactose in different types of milk and milk products are presented. The amounts of micro- and macronutrients in them are compared with the daily requirements established by accepted sources. Foods are suggested that can play vital roles in permanently avoiding lactose-containing dairy products, for example, brussels sprouts, as a good source of vitamin B1; kale, as a source of vitamin K; and cereals at breakfast for vitamin B6. Attention is paid to mature cheeses as they are extremely beneficial for health due to their rich vitamin and elemental compositions, and they are also suitable for people with lactose intolerance due to their low lactose content. This information is rarely provided on packaging. In addition, the current state of labeling for the presence of lactose in food and pharmaceutical products is discussed. The term “hidden lactose” is introduced to include added lactose in unexpected foods, drinks, and even medicines. Full article
(This article belongs to the Section Milk and Human Health)
12 pages, 700 KiB  
Article
Probiotic Milk and Oat Beverages with Increased Protein Content: Survival of Probiotic Bacteria Under Simulated In Vitro Digestion Conditions
by Małgorzata Pawlos, Katarzyna Szajnar and Agata Znamirowska-Piotrowska
Nutrients 2024, 16(21), 3673; https://doi.org/10.3390/nu16213673 - 29 Oct 2024
Viewed by 628
Abstract
Background: The increasing prevalence of plant-based dietary preferences, driven by lactose intolerance, allergies, and adherence to vegan diets, has necessitated the exploration of alternative food matrices for probiotic delivery. Objectives: This study aimed to evaluate the effects of whey protein isolate, pea protein [...] Read more.
Background: The increasing prevalence of plant-based dietary preferences, driven by lactose intolerance, allergies, and adherence to vegan diets, has necessitated the exploration of alternative food matrices for probiotic delivery. Objectives: This study aimed to evaluate the effects of whey protein isolate, pea protein isolate, and soy protein isolate on the viability of L. casei and L. johnsonii during simulated in vitro gastrointestinal digestion. Furthermore, the study investigated the impact of two distinct matrices—cow’s milk and an oat-based beverage—on the survival of these probiotic strains. Fermented products were prepared using cow’s milk and an oat-based beverage as matrices, with simulated digestion performed following a seven-day storage period at 5 °C. The in vitro digestion model encompassed oral, gastric, and small intestinal phases, with probiotic viability assessed using the plate-deep method at each stage. Methods: Before digestion, L. casei exhibited higher populations than L. johnsonii in both matrices. Including 3% soy and pea protein, isolates promoted the growth of L. casei in both fermented milk and oat beverages. However, a marked reduction in probiotic viability was observed during the gastric phase, with L. casei counts decreasing by 6.4–7.8 log cfu g−1 in fermented milk and 3.1–4 log cfu g−1 in oat beverages, while L. johnsonii demonstrated similar reductions. Conclusion: These findings underscore the protective role of dairy components on probiotic viability, while the oat-based matrix exhibited a reduced capacity for sustaining probiotic populations throughout digestion. Future research should focus on optimizing plant-based matrices to enhance probiotic stability during gastrointestinal transit. Full article
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<p>Procedure of in vitro digestion process.</p>
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<p>The survival rate of <span class="html-italic">L. johnsonii</span> and <span class="html-italic">L. casei</span> in fermented beverages depending on the isolate additive applied. WPI—whey protein isolate; SPI—soy protein isolate; PPI—pea protein isolate.</p>
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23 pages, 2192 KiB  
Article
Enhanced Labeling to Promote Consumption of Nutrient Dense Foods and Healthier Diets
by Charles Benbrook and Robin Mesnage
Foods 2024, 13(21), 3377; https://doi.org/10.3390/foods13213377 - 24 Oct 2024
Viewed by 1254
Abstract
Background/Objectives: Efforts are underway worldwide to design and deploy food labeling systems that provide consumers with the information needed to shift dietary patterns toward nutrient dense, healthier foods. Despite a compelling need for progress, worrisome public health trends persist that are rooted in [...] Read more.
Background/Objectives: Efforts are underway worldwide to design and deploy food labeling systems that provide consumers with the information needed to shift dietary patterns toward nutrient dense, healthier foods. Despite a compelling need for progress, worrisome public health trends persist that are rooted in the popularity of unhealthy, heavily processed foods. Methods: The nutrition and health-related content on the packaging of nine common foods sold in the US and Europe is analyzed and compared. The current scope of nutrient-specific messaging is characterized, including messages highlighting health-related benefits stemming from the mix and levels of mostly macronutrients in food products. Results: An average of 6.9 unique nutrition-related messages appear on the packaging of nine US food products, while EU food products contain an average of 5.0. Messaging around the ingredients in food products accounts for the largest share, e.g., “100% whole grain”, “Vegan”, and “No artificial preservatives”. The macronutrients of fat, fiber, cholesterol, salt, sugar, and protein are the focus of most messaging around health benefits. The degree of food processing and essential vitamin, mineral, and phytochemical micronutrients receive little or no attention, despite their importance in positive health outcomes. Conclusions: Current nutrition-related labeling fails to inform consumers of the enormous differences in the contribution of food products in meeting nutritional needs. Existing metrics and rating systems do not effectively account for the critical relationship between nutrient density and caloric content. Existing metrics and systems do not reflect the impacts of processing on food nutritional quality in ways that provide consumers meaningful information. New concepts, metrics, and label elements are described that could promote healthier dietary patterns. Clear and mandatory nutrition labeling could begin shifting market share toward healthier options, and this could trigger and guide changes in manufactured food recipes that make brand-name products healthier, benefiting all consumers. Full article
(This article belongs to the Section Food Nutrition)
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<p>Graphics displaying macronutrient composition under consideration for front-of-pack nutrition labeling by the US FDA.</p>
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<p>Boxes Providing Information on Macronutrient Composition on Products Sold in the US and EU.</p>
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<p>A Front-of-Pack Nutritional Quality Continuum Based on the NuCal Value for Special K Cereal.</p>
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<p>Food Processing Graphic for Placement on Front-of-Pack Labeling.</p>
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<p>Conveying the Nutritional Quality and Degree of Food Processing in a Food Product in an Integrated Graphic.</p>
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15 pages, 275 KiB  
Article
A Qualitative Study of Rural Plant-Based Eaters’ Knowledge and Practices for Nutritional Adequacy
by Michelle Leonetti, Jane Kolodinsky, Amy Trubek and Emily H. Belarmino
Nutrients 2024, 16(20), 3504; https://doi.org/10.3390/nu16203504 - 16 Oct 2024
Viewed by 906
Abstract
(1) Background: Healthful plant-based diets, especially those rich in minimally processed plant-based foods such as fruits, vegetables, and whole grains, have been associated with a lower risk of diet-related chronic disease. However, individuals who limit or avoid animal products may be at risk [...] Read more.
(1) Background: Healthful plant-based diets, especially those rich in minimally processed plant-based foods such as fruits, vegetables, and whole grains, have been associated with a lower risk of diet-related chronic disease. However, individuals who limit or avoid animal products may be at risk of nutrient deficiencies, especially related to Vitamin B12, Vitamin D, omega-3 fatty acids, calcium, iron, iodine, zinc, and protein. Such deficiencies can result in both short- and long-term health challenges. We used qualitative methods to explore plant-based eaters’ knowledge and awareness of these eight nutrients of concern in diets that limit or exclude animal-source foods. (2) Methods: We conducted interviews with 28 rural flexitarian, pescatarian, vegan, or vegetarian adults in Vermont, USA. (3) Results: The participants positively viewed the healthfulness of plant-based diets, but many recognized limitations to accessing Vitamin B12, Vitamin D, and omega-3 fatty acids. They shared the strategies used to meet their needs including eating a varied diet, seeking out specific foods, and taking dietary supplements. Analyses identified gaps in the participants’ knowledge related to nutrient bioavailability, food sources of specific nutrients, and the importance of zinc and iodine. Vegans—the highest-risk group—generally presented as the most knowledgeable. The participants noted a lack of local plant-based nutrition expertise. (4) Conclusions: Addressing the identified knowledge gaps and challenges to dietary adequacy, especially among those who limit, but do not fully omit, animal-source foods, may support plant-based nutrition. Full article
(This article belongs to the Section Nutrition and Public Health)
19 pages, 1706 KiB  
Article
Plant-Based Beverages: Consumption Habits, Perception and Knowledge on a Sample of Portuguese Citizens
by Ofélia Anjos, Patrícia C. P. Pires, Joana Gonçalves, Letícia M. Estevinho, António G. Mendonça and Raquel P. F. Guiné
Foods 2024, 13(20), 3235; https://doi.org/10.3390/foods13203235 - 11 Oct 2024
Viewed by 647
Abstract
Plant-based beverages (PBB) consumption has increased significantly worldwide due to an interest in vegetarian/vegan diets, taste preferences, health and ethical and environmental issues. Therefore, this study intends to investigate consumption habits, consumer preferences and consumers’ level of knowledge about PBB. In this study, [...] Read more.
Plant-based beverages (PBB) consumption has increased significantly worldwide due to an interest in vegetarian/vegan diets, taste preferences, health and ethical and environmental issues. Therefore, this study intends to investigate consumption habits, consumer preferences and consumers’ level of knowledge about PBB. In this study, a voluntary, anonymous questionnaire survey was applied to a sample of participants from Portugal. The sample was recruited by convenience, and therefore, the distribution among the groups was not even. Data analysis involved different statistical techniques: basic statistics, chi-square tests, factor analysis, cluster analysis and tree classification analysis. The results indicated that the most consumed PBB were almond, soy and oat beverages. The majority of consumers chose these beverages for nutritional and health reasons, while a smaller number consumed them as part of a vegetarian or vegan diet. The main motivations for consuming PBB are mainly associated with sustainability and health benefits. The results regarding the respondents’ knowledge about PBB revealed that a health-related profession was the most significant predictor. These results allowed us to conclude that the factors of nutrition, health, ethics and practice of a vegetarian/vegan diet influence the consumption of PBB. It was also concluded that being in a healthcare profession, along with age and professional status within this field, were significant factors influencing the level of knowledge about PBB. Full article
(This article belongs to the Section Drinks and Liquid Nutrition)
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<p>Geographical distribution of the participants.</p>
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<p>Preferences regarding the type of PBB consumed (N = 481).</p>
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<p>Consumption of some PBB according to age group (<sup>1</sup> <span class="html-italic">p</span>-value of the chi-square test).</p>
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<p>Tree classification for the level of knowledge about PBB.</p>
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12 pages, 769 KiB  
Article
Assessment of the Nutritional Value and Antioxidant Properties of Plant-Based Yogurt from Chickpeas
by Grażyna Budryn and Joanna Grzelczyk
Appl. Sci. 2024, 14(20), 9228; https://doi.org/10.3390/app14209228 - 11 Oct 2024
Viewed by 776
Abstract
Plant-based yogurts are becoming more and more popular. These are vegan products for people with food allergies or intolerances, athletes, and ordinary consumers. The use of chickpea “milk” allows one to obtain a plant-based yogurt with a high protein content, dietary fiber and [...] Read more.
Plant-based yogurts are becoming more and more popular. These are vegan products for people with food allergies or intolerances, athletes, and ordinary consumers. The use of chickpea “milk” allows one to obtain a plant-based yogurt with a high protein content, dietary fiber and antioxidant potential. This study assessed the content of polyphenols, selected nutrients, and antioxidant properties in chickpea yogurt. The vegetable chickpea yogurt was rich in protein (4.34–7.90 g/100 g) and dietary fiber (2–4%). It was also characterized by a high antioxidant activity (DPPH = 2.61 to 4.32 μmol TE/g, FRAP = 3.11 to 48.33 μmol TE/g). The use of 3-day soaking and inoculation with Vegurt or a kefir bacterial inoculum allowed us to obtain a high protein content. The addition of honey increased the polyphenol content and antioxidant potential of the plant-based yogurt (139–148 GAE mg/100 g). This research will be used in future research into optimizing the conditions for obtaining plant-based yogurts with chickpeas. Further research should include models for testing health-promoting properties. Full article
(This article belongs to the Special Issue Extraction of Functional Ingredients and Their Application)
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<p>Samples after 14 h of incubation.</p>
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<p>Ash content in chickpea yogurts. Values are expressed as mean value ± SD; <span class="html-italic">n</span> = 3; values with the same superscript letter (a–e) along the same column are not significantly different (<span class="html-italic">p</span> &gt; 0.05).</p>
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<p>FRAP and DPPH antioxidant capacity analysis. Values are expressed as mean value ± SD; <span class="html-italic">n</span> = 3; values with the same superscript letter (a–d) along the same column are not significantly different (<span class="html-italic">p</span> &gt; 0.05).</p>
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