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17 pages, 8569 KiB  
Article
Transforming Prediction into Decision: Leveraging Transformer-Long Short-Term Memory Networks and Automatic Control for Enhanced Water Treatment Efficiency and Sustainability
by Cheng Qiu, Qingchuan Li, Jiang Jing, Ningbo Tan, Jieping Wu, Mingxi Wang and Qianglin Li
Sensors 2025, 25(6), 1652; https://doi.org/10.3390/s25061652 (registering DOI) - 7 Mar 2025
Abstract
The study addresses the critical issue of accurately predicting ammonia nitrogen (NH3-N) concentration in a sequencing batch reactor (SBR) system, achieving reduced consumption through automatic control technology. NH3-N concentration serves as a key indicator of treatment efficiency and environmental [...] Read more.
The study addresses the critical issue of accurately predicting ammonia nitrogen (NH3-N) concentration in a sequencing batch reactor (SBR) system, achieving reduced consumption through automatic control technology. NH3-N concentration serves as a key indicator of treatment efficiency and environmental impact; however, its complex dynamics and the scarcity of measurements pose significant challenges for accurate prediction. To tackle this problem, an innovative Transformer-long short-term memory (Transformer-LSTM) network model was proposed, which effectively integrates the strengths of both Transformer and LSTM architectures. The Transformer component excels at capturing long-range dependencies, while the LSTM component is adept at modeling sequential patterns. The innovation of the proposed methodology resides in the incorporation of dissolved oxygen (DO), electrical conductivity (EC), and oxidation-reduction potential (ORP) as input variables, along with their respective rate of change and cumulative value. This strategic selection of input features enhances the traditional utilization of water quality indicators and offers a more comprehensive dataset for prediction, ultimately improving model accuracy and reliability. Experimental validation on NH3-N datasets from the SBR system reveals that the proposed model significantly outperforms existing advanced methods in terms of root mean squared error (RMSE), mean absolute error (MAE), and coefficient of determination (R2). Furthermore, by integrating real-time sensor data with the Transformer-LSTM network and automatic control, substantial improvements in water treatment processes were achieved, resulting in a 26.9% reduction in energy or time consumption compared with traditional fixed processing cycles. This methodology provides an accurate and reliable tool for predicting NH3-N concentrations, contributing significantly to the sustainability of water treatment and ensuring compliance with emission standards. Full article
(This article belongs to the Topic Water and Energy Monitoring and Their Nexus)
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<p>LSTM neural network structure.</p>
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<p>Transformer-LSTM inner-unit network structure.</p>
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<p>Transformer-LSTM outer-unit network structure.</p>
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<p>Treatment process of SBR.</p>
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<p>Typical variation of NH<sub>3</sub>-N, EC, DO, and ORP during a fixed process cycle.</p>
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<p>Typical processed input variables of EC, DO, and ORP during a fixed process cycle.</p>
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<p>Prediction errors of all methods.</p>
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<p>Prediction results of the Transformer-LSTM method.</p>
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<p>The technical balance between the SBR and machine learning.</p>
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<p>Predicted NH<sub>3</sub>-N by Transformer-LSTM network and process range on 20 cycles (All horizontal axes represent time (min), and all vertical axes represent NH<sub>3</sub>-N (mg/L).</p>
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23 pages, 20698 KiB  
Article
Numerical Study on the Bending Performance of Steel-Ribbed Composite Slabs for Substations
by Lin Li, Yong Liu, Zhenzhong Wei, Yunan Jiang, Haomiao Chen, Yu Zhang, Chen Liu, Kunjie Rong and Li Tian
Appl. Sci. 2025, 15(6), 2876; https://doi.org/10.3390/app15062876 (registering DOI) - 7 Mar 2025
Abstract
This study investigates the bending behavior of steel-ribbed composite slabs for a 500 kV substation project in China through numerical simulation. The unidirectional bending performance of the slab was first analyzed and validated against theoretical calculations. After that, the bidirectional bending performance of [...] Read more.
This study investigates the bending behavior of steel-ribbed composite slabs for a 500 kV substation project in China through numerical simulation. The unidirectional bending performance of the slab was first analyzed and validated against theoretical calculations. After that, the bidirectional bending performance of double-spliced and triple-spliced composite slabs were evaluated against the monolithic slab, followed by a parametric analysis to identify the influence of key factors. The results indicate that the steel-ribbed composite slabs feature high cracking strength, post-crack stiffness, bearing capacity, and commendable ductility under both unidirectional and bidirectional loading conditions. Under unidirectional loading, the ultimate capacity of the slab reaches 57–58 kN/m2. Under bidirectional loading, the cracking load and bearing capacity of the dense-splicing composite slabs increase by more than 60% compared with unidirectional loading. Composite and monolithic slabs exhibit similar crack patterns and ultimate capacities under bidirectional loading; however, the presence of splicing joints results in a slight increase in the ultimate deflection of the double-spliced and triple-spliced composite slabs by 7.53% and 7.75% compared with that of the monolithic slab. The ratio of prestressing steel is identified as the most critical parameter for failure control, followed by the concrete strength. When the strength of the joint-connecting rebars exceeds 235 MPa and the diameter is greater than 4 mm, transversal force transfer across the joints is reliable. This paper provides valuable insights and practical guidance for the prefabricated construction of substations. Full article
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<p>Daocheng (Huangbuling) 500 kV substation. (<b>a</b>) Aerial view; (<b>b</b>) Layout plan for the 8.800 m floor (mm).</p>
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<p>Illustration of the steel-ribbed composite slab (mm).</p>
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<p>Detailed drawing of precast base slabs A and B (mm).</p>
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<p>Double-spliced, triple-spliced, and cast-in-place monolithic slab (mm).</p>
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<p>Constitutive models: (<b>a</b>) Response of concrete in compression; (<b>b</b>) Concrete compressive damage; (<b>c</b>) Response of concrete in tension; (<b>d</b>) Concrete tensile damage; (<b>e</b>) Response of rebar in tension; (<b>f</b>) Response of prestressing steel in tension.</p>
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<p>FE model of steel-ribbed composite slab A.</p>
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<p>FE model of steel-ribbed composite slab B.</p>
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<p>FE model of the double-spliced composite slab: (<b>a</b>) After composition; (<b>b</b>) Transverse joint-connecting rebars before composition.</p>
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<p>FE model of the triple-spliced composite slab: (<b>a</b>) After composition; (<b>b</b>) Transverse joint-connecting rebars before composition.</p>
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<p>FE model of the cast-in-place monolithic slab: (<b>a</b>) After construction; (<b>b</b>) Reinforcement.</p>
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<p>Comparison of load–deflection curves between test and FE results: (<b>a</b>) Uniform loading of the precast base slab [<a href="#B24-applsci-15-02876" class="html-bibr">24</a>]; (<b>b</b>) Four-point loading of the composite slab [<a href="#B14-applsci-15-02876" class="html-bibr">14</a>].</p>
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<p>Analysis results of composite slab A under unidirectional loading: (<b>a</b>) Deflection at cracking; (<b>b</b>) Deflection at failure; (<b>c</b>) Initial bottom cracks at cracking; (<b>d</b>) Distribution of bottom cracks at failure; (<b>e</b>) Stress in prestressing steel at cracking; (<b>f</b>) Stress in prestressing steel at failure.</p>
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<p>Analysis results of composite slab B under unidirectional loading: (<b>a</b>) Deflection at cracking; (<b>b</b>) Deflection at failure; (<b>c</b>) Initial bottom cracks at cracking; (<b>d</b>) Distribution of bottom cracks at failure; (<b>e</b>) Stress in prestressing steel at cracking; (<b>f</b>) Stress in prestressing steel at failure.</p>
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<p>Load–deflection curves for steel-ribbed composite slabs at mid-span.</p>
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<p>Analysis results of double-spliced composite bidirectional slab: (<b>a</b>) Deflection at cracking; (<b>b</b>) Deflection at failure; (<b>c</b>) Initial bottom cracks at cracking; (<b>d</b>) Distribution of bottom cracks at failure; (<b>e</b>) Stress in prestressing steel at cracking; (<b>f</b>) Stress in prestressing steel at failure; (<b>g</b>) Stress of transverse rebar stress at cracking; (<b>h</b>) Stress of transverse rebar stress at failure.</p>
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<p>Analysis results of double-spliced composite bidirectional slab: (<b>a</b>) Deflection at cracking; (<b>b</b>) Deflection at failure; (<b>c</b>) Initial bottom cracks at cracking; (<b>d</b>) Distribution of bottom cracks at failure; (<b>e</b>) Stress in prestressing steel at cracking; (<b>f</b>) Stress in prestressing steel at failure; (<b>g</b>) Stress of transverse rebar stress at cracking; (<b>h</b>) Stress of transverse rebar stress at failure.</p>
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<p>Load–stress curves for reinforcement at the center of the slab: (<b>a</b>) Prestressing steel; (<b>b</b>) Joint-connecting rebar.</p>
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<p>Analysis results of triple-spliced composite bidirectional slab: (<b>a</b>) Deflection at cracking; (<b>b</b>) Deflection at failure; (<b>c</b>) Initial bottom cracks at cracking; (<b>d</b>) Distribution of bottom cracks at failure; (<b>e</b>) Stress in prestressing steel at cracking; (<b>f</b>) Stress in prestressing steel at failure; (<b>g</b>) Stress of transverse rebar stress at cracking; (<b>h</b>) Stress of transverse rebar stress at failure.</p>
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<p>Analysis results of triple-spliced composite bidirectional slab: (<b>a</b>) Deflection at cracking; (<b>b</b>) Deflection at failure; (<b>c</b>) Initial bottom cracks at cracking; (<b>d</b>) Distribution of bottom cracks at failure; (<b>e</b>) Stress in prestressing steel at cracking; (<b>f</b>) Stress in prestressing steel at failure; (<b>g</b>) Stress of transverse rebar stress at cracking; (<b>h</b>) Stress of transverse rebar stress at failure.</p>
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<p>Load–stress curves for reinforcement at the center of the slab: (<b>a</b>) Prestressing steel; (<b>b</b>) Joint-connecting rebar.</p>
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<p>Analysis results of the cast-in-place bidirectional slab: (<b>a</b>) Deflection at cracking; (<b>b</b>) Deflection at failure; (<b>c</b>) Initial bottom cracks at cracking; (<b>d</b>) Distribution of bottom cracks at failure; (<b>e</b>) Stress in prestressing steel at cracking; (<b>f</b>) Stress in prestressing steel at failure; (<b>g</b>) Stress of transverse rebar stress at cracking; (<b>h</b>) Stress of transverse rebar stress at failure.</p>
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<p>Load–stress curves for reinforcement at the center of the slab: (<b>a</b>) Prestressing steel; (<b>b</b>) Joint-connecting rebar.</p>
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<p>Load–deflection curves for bidirectional slabs.</p>
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<p>Failure modes of dense splicing slabs: (<b>a</b>) Yielding of the longitudinal prestressing steel; (<b>b</b>) Concrete crushing; (<b>c</b>) Yielding of the transverse joint-connecting steel.</p>
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<p>Parametric analysis results of the triple-spliced composite slab: (<b>a</b>) Concrete strength; (<b>b</b>) Ratio of the longitudinal prestressing steel; (<b>c</b>) Strength of the joint-connecting rebar; (<b>d</b>) Diameter of the joint-connecting rebar.</p>
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13 pages, 5323 KiB  
Article
Advances in the Detection and Identification of Bacterial Biofilms Through NIR Spectroscopy
by Cristina Allende-Prieto, Lucía Fernández, Pablo Rodríguez-Gonzálvez, Pilar García, Ana Rodríguez, Carmen Recondo and Beatriz Martínez
Foods 2025, 14(6), 913; https://doi.org/10.3390/foods14060913 (registering DOI) - 7 Mar 2025
Abstract
Bacterial biofilms play an important role in the pathogenesis of infectious diseases but are also very relevant in other fields such as the food industry. This fact has led to an increased focus on the early identification of these structures as prophylaxes to [...] Read more.
Bacterial biofilms play an important role in the pathogenesis of infectious diseases but are also very relevant in other fields such as the food industry. This fact has led to an increased focus on the early identification of these structures as prophylaxes to prevent biofilm-related contaminations or infections. One of the objectives of the present study was to assess the effectiveness of NIR (Near Infrared) spectroscopy in the detection and differentiation of biofilms from different bacterial species, namely Staphylococcus epidermidis, Staphylococcus aureus, Enterococcus faecium, Salmonella Typhymurium, Escherichia coli, Listeria monocytogenes, and Lactiplantibacillus plantarum. Additionally, we aimed to examine the capability of this technology to specifically identify S. aureus biofilms on glass surfaces commonly used as storage containers and processing equipment. We developed a detailed methodology for data acquisition and processing that takes into consideration the biochemical composition of these biofilms. To improve the quality of the spectral data, SNV (Standard Normal Variate) and Savitzky–Golay filters were applied, which correct systematic variations and eliminate random noise, followed by an exploratory analysis that revealed significant spectral differences in the NIR range. Then, we performed principal component analysis (PCA) to reduce data dimensionality and, subsequently, a Random Forest discriminant statistical analysis was used to classify biofilms accurately and reliably. The samples were organized into two groups, a control set and a test set, for the purpose of performing a comparative analysis. Model validation yielded an accuracy of 80.00% in the first analysis (detection and differentiation of biofilm) and 93.75% in the second (identification of biofilm on glass surfaces), thus demonstrating the efficacy of the proposed method. These results demonstrate that this technique is effective and reliable, indicating great potential for its application in the field of biofilm detection. Full article
(This article belongs to the Section Food Microbiology)
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<p>Spectral signatures obtained after NIR measurement of each bacterial biofilm. Bacterial species and control are indicated on the bottom left.</p>
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<p>Random Forest performance: Influence of <span class="html-italic">mtry</span> on accuracy and stability.</p>
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<p>Distribution of the bacterial samples.</p>
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<p>Performance metrics of the Random Forest model.</p>
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<p>Average spectral signatures of contaminated and uncontaminated samples.</p>
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<p>Principal component analysis: cumulative variance explained.</p>
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21 pages, 825 KiB  
Article
The Chinese Adaptation of the Teachers’ Sense of Efficacy Scale in Early Childhood Pre-Service Teachers: Validity, Measurement Invariance, and Reliability
by Mingxing Shao, Mohd Mokhtar Muhamad, Fazilah Razali, Nasnoor Juzaily Mohd Nasiruddin, Xinchong Sha and Guoqiang Yin
Behav. Sci. 2025, 15(3), 329; https://doi.org/10.3390/bs15030329 (registering DOI) - 7 Mar 2025
Abstract
Teachers’ sense of efficacy (TSE) is a crucial construct for evaluating the quality of pre-service teachers. While the Teachers’ Sense of Efficacy Scale (TSES) is the most widely used and promising instrument for measuring TSE, there is no existing literature assessing the appropriateness [...] Read more.
Teachers’ sense of efficacy (TSE) is a crucial construct for evaluating the quality of pre-service teachers. While the Teachers’ Sense of Efficacy Scale (TSES) is the most widely used and promising instrument for measuring TSE, there is no existing literature assessing the appropriateness of the TSES for early childhood pre-service teachers in China. This study aimed to adapt the English version of the TSES for the Chinese early childhood education contexts, testing its factor structure, validity, measurement invariance, and reliability. The sample included 402 participants in China. The TSES was translated into Chinese using the standard back-to-back translation method. CFA results indicated that the TSES is best represented by a modified three-factor model, demonstrating strong preliminary, overall, and internal structure fit. The concurrent validity, convergent validity, criterion-related validity, internal consistency reliability, and composite reliability of the Chinese version of the TSES were robust. The measurement invariance across age and college year was also confirmed. This study addresses a gap in the literature by providing robust empirical evidence on the factor structure, validity, measurement invariance, and reliability of the Chinese version of the TSES for early childhood pre-service teachers, thereby enhancing understanding of TSE in Chinese-speaking Confucian culture and in early childhood education contexts. Full article
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<p>The best-fit model of TSES-SF (Model 4). Note. IS: “Efficacy for instructional strategies”; CM: “Efficacy for classroom management”; SE: “Efficacy for student engagement”.</p>
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11 pages, 467 KiB  
Article
A Hubble Constant Determination Through Quasar Time Delays and Type Ia Supernovae
by Leonardo R. Colaço
Universe 2025, 11(3), 89; https://doi.org/10.3390/universe11030089 (registering DOI) - 7 Mar 2025
Abstract
This paper presents a new model-independent constraint on the Hubble constant (H0) by anchoring relative distances from Type Ia supernovae (SNe Ia) observations to absolute distance measurements from time-delay strong Gravitational Lensing (SGL) systems. The approach only uses the validity [...] Read more.
This paper presents a new model-independent constraint on the Hubble constant (H0) by anchoring relative distances from Type Ia supernovae (SNe Ia) observations to absolute distance measurements from time-delay strong Gravitational Lensing (SGL) systems. The approach only uses the validity of the cosmic distance duality relation (CDDR) to derive constraints on H0. By using Gaussian Process (GP) regression to reconstruct the unanchored luminosity distance from the Pantheon+ compilation to match the time-delay angular diameter distance at the redshift of the lenses, one yields a value of H0=75.57±4.415 km/s/Mpc at a 68% confidence level. The result aligns well with the local estimate from Cepheid variables within the 1σ confidence region, indicating consistency with late-universe probes. Full article
(This article belongs to the Special Issue Current Status of the Hubble Tension)
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<p>(<b>Left</b>): The GP reconstruction of <math display="inline"><semantics> <mrow> <mi mathvariant="normal">Θ</mi> <mrow> <mo>(</mo> <mi>z</mi> <mo>)</mo> </mrow> <mo>≡</mo> <msub> <mi>H</mi> <mn>0</mn> </msub> <msub> <mi>D</mi> <mi>L</mi> </msub> <mrow> <mo>(</mo> <mi>z</mi> <mo>)</mo> </mrow> </mrow> </semantics></math> using the SNe Ia Pantheon+ compilation [<a href="#B44-universe-11-00089" class="html-bibr">44</a>]. (<b>Right</b>): The 15 selected <math display="inline"><semantics> <mrow> <msubsup> <mi>D</mi> <mrow> <mi>A</mi> <mo>,</mo> <mo>Δ</mo> <mi>t</mi> </mrow> <mi>SGL</mi> </msubsup> <mrow> <mo>(</mo> <msub> <mi>z</mi> <mi>l</mi> </msub> <mo>,</mo> <msub> <mi>z</mi> <mi>s</mi> </msub> <mo>)</mo> </mrow> </mrow> </semantics></math> data points according to <math display="inline"><semantics> <msub> <mi>z</mi> <mi>l</mi> </msub> </semantics></math> from [<a href="#B31-universe-11-00089" class="html-bibr">31</a>,<a href="#B45-universe-11-00089" class="html-bibr">45</a>].</p>
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<p>The posterior probability distribution function for the free parameter <math display="inline"><semantics> <msub> <mi>H</mi> <mn>0</mn> </msub> </semantics></math>, with a best-fit value of <math display="inline"><semantics> <mrow> <mn>75.57</mn> <mo>±</mo> <mn>4.415</mn> </mrow> </semantics></math> km/s/Mpc at the <math display="inline"><semantics> <mrow> <mn>1</mn> <mi>σ</mi> </mrow> </semantics></math> confidence level. The grey and blue vertical dashed lines represent the estimates from Planck [<a href="#B11-universe-11-00089" class="html-bibr">11</a>] and Riess [<a href="#B4-universe-11-00089" class="html-bibr">4</a>], respectively, along with their corresponding <math display="inline"><semantics> <mrow> <mn>1</mn> <mi>σ</mi> </mrow> </semantics></math> confidence regions. The light green horizontal dashed lines indicate the <math display="inline"><semantics> <mrow> <mn>1</mn> <mi>σ</mi> </mrow> </semantics></math> and <math display="inline"><semantics> <mrow> <mn>2</mn> <mi>σ</mi> </mrow> </semantics></math> confidence levels.</p>
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16 pages, 2010 KiB  
Article
Locked and Loaded: Divergent Handgrip Tests as Surrogate Measures for One-Repetition Maximal Strength
by S. Kyle Travis, Antonella V. Schwarz and Benjamin I. Burke
Biomechanics 2025, 5(1), 16; https://doi.org/10.3390/biomechanics5010016 - 7 Mar 2025
Abstract
Background/Objectives: Despite widespread use in clinical and athletic settings, validity of handgrip strength (HGS) as a surrogate for maximal strength remains debated, particularly regarding how testing posture influences its predictive value. Moreover, while HGS is frequently considered a marker of ‘total strength’, this [...] Read more.
Background/Objectives: Despite widespread use in clinical and athletic settings, validity of handgrip strength (HGS) as a surrogate for maximal strength remains debated, particularly regarding how testing posture influences its predictive value. Moreover, while HGS is frequently considered a marker of ‘total strength’, this term is often vaguely defined, lacking a clear, performance-based framework. Therefore, this study investigates HGS as a potential surrogate measure for one-repetition maximum (1RM) performances in key compound lifts via back squat (BS), bench press (BP), deadlift (DL), and total (TOT), while accounting for variations in testing posture. Methods: Two distinct testing conditions were used to account for postural influences: Experiment 1 implemented high-output standing HGS (HGSSTAND) in 22 recreationally trained males [Wilks Score: 318.51 ± 44.61 au] vs. Experiment 2, which included low-output seated HGS (HGSSIT) in 22 competitive powerlifters [409.86 ± 46.76 au], with all testing immediately followed by 1RM assessment. Results: Correlational analyses identified the strongest association between HGSSTAND and 1RM DL (r = 0.693, BF10 = 106.42), whereas HGSSIT exhibited the strongest relationship with 1RM BP (r = 0.732, BF10 = 291.32). Postural effects had a significant impact on HGS outcomes (p < 0.001, η2 = 0.413), with HGSSTAND producing higher outputs than HGSSIT despite lower absolute strength 1RM capabilities. Conclusions: These findings emphasize the role of biomechanical specificity and neuromuscular engagement in grip strength assessments, indicating that HGS can function as a practical surrogate for maximal strength, though its predictive value depends on posture. Strength practitioners, sport scientists, and clinicians should consider these confounding factors when implementing HGS-based monitoring strategies. Full article
(This article belongs to the Special Issue Biomechanics in Sport, Exercise and Performance)
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<p>Sample distribution for one-repetition-maximum (1RM) back squat (<b>A</b>), bench press (<b>B</b>), deadlift (<b>C</b>), and total (<b>D</b>).</p>
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<p>Testing timeline begins with initial laboratory testing via (<b>A</b>) hydration, height, and body mass check preceding (<b>B1</b>) standing or (<b>B2</b>) seated handgrip strength testing followed by a (<b>C1</b>) dynamic warm-up and 1RMs on (<b>C2</b>) back squat, (<b>C3</b>) bench press, and (<b>C4</b>) deadlift.</p>
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<p>Classical correlations: standing handgrip strength (HGS<sub>STAND</sub>) vs. one-repetition-maximum (1RM) for (<b>A</b>) back squat, (<b>B</b>) bench press, (<b>C</b>) deadlift, and (<b>D</b>) total. Black solid lines represent the line of best fit, while blue dashed lines indicate the 95% confidence intervals. Each point represents an individual data observation.</p>
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<p>Classical correlations: seated handgrip strength (HGS<sub>SIT</sub>) vs. one-repetition-maximum (1RM) for (<b>A</b>) back squat, (<b>B</b>) bench press, (<b>C</b>) deadlift, and (<b>D</b>) total. Black solid lines represent the line of best fit, while blue dashed lines indicate the 95% confidence intervals. Each point represents an individual data observation.</p>
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16 pages, 9628 KiB  
Article
Genome-Wide Identification of the NAC Gene Family in Brassica rapa (L.) and Expression Pattern Analysis of BrNAC2s
by Weiqiang Li, Fan Ping, Huixuan Jiang, Shuqing Zhang, Tong Zhao, Kaiwen Liu, Hongrui Yu, Iqbal Hussian, Xiliang Ren and Xiaolin Yu
Plants 2025, 14(6), 834; https://doi.org/10.3390/plants14060834 (registering DOI) - 7 Mar 2025
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Abstract
Flowers are one of the most important organs in plants. Their development serves as a key indicator of the transition from vegetative to reproductive growth and is regulated by various internal signals and environmental factors. NAC (NAM, ATAF, CUC) transcription factors (TFs) play [...] Read more.
Flowers are one of the most important organs in plants. Their development serves as a key indicator of the transition from vegetative to reproductive growth and is regulated by various internal signals and environmental factors. NAC (NAM, ATAF, CUC) transcription factors (TFs) play a crucial regulatory role in floral organ development; however, research on the analysis and identification of the NAC TF family in Chinese cabbage (Brassica rapa L.) remains limited. In this study, we performed a comprehensive genome-wide analysis of NACs in B. rapa and identified 279 members of the BrNAC gene family. Their physicochemical properties, domain structure, collinearity relation, and cis-regulatory elements were evaluated. Phylogenetic analysis indicates that NAC proteins from Arabidopsis, B. rapa, B. oleracea, and B. nigra can be classified into seven distinct clades. BrNACs exhibit a tissue-specific expression, and nine BrNACs being specifically expressed in the inflorescence. Furthermore, nine flower-related BrNACs were selected for RT-qPCR analysis to validate their expression profiles. BrNAC2s has been cloned to investigate their subcellular localization, and examine the expression patterns of their promoters in Arabidopsis inflorescences. BrNAC2a and BrNAC2c are highly expressed in stamens while BrNAC2b exhibits elevated expression in pistils and pedicel. Collectively, our findings enhance the understanding of the BrNAC family and provide a foundation for future studies on the molecular mechanisms of BrNACs in floral development. Full article
(This article belongs to the Section Plant Genetics, Genomics and Biotechnology)
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<p>Chromosome distribution of <span class="html-italic">BrNAC</span> genes. The distribution of 279 <span class="html-italic">BrNAC</span> genes on ten <span class="html-italic">B. rapa</span> chromosomes.</p>
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<p>Phylogenetic analysis of NAC proteins in <span class="html-italic">B. rapa</span>. Phylogenetic analyses were conducted on NAC proteins from <span class="html-italic">B. rapa</span>, <span class="html-italic">A. thaliana</span>, <span class="html-italic">B. oleracea</span>, and <span class="html-italic">B. nigra</span>. MUSCLE was used for multiple sequence alignment. Phylogenetic trees were constructed using the Maximum Likelihood (ML) method with 1000 bootstrap repeats. The resulting phylogenetic tree was visualized with the online tool iTOL 6.9.1 (<a href="https://itol.embl.de/" target="_blank">https://itol.embl.de/</a>, accessed on 20 September 2024). Different colors indicate different NAC subgroups.</p>
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<p>Collinear gene pair analysis of <span class="html-italic">BrNACs</span> between <span class="html-italic">A. thaliana</span>, <span class="html-italic">B. oleracea</span> and <span class="html-italic">B. nigra</span>. The red line represents collinear gene pairs. The orange color represents the <span class="html-italic">A. thaliana</span> chromosome, the green color represents the <span class="html-italic">B. rapa</span> chromosome, and the pink color represents the <span class="html-italic">B. oleracea</span> chromosome, the blue color represents the <span class="html-italic">B. nigra</span> chromosome.</p>
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<p>Cis-elements in the 2 kb promoter sequences of <span class="html-italic">BrNAC</span> genes. Different colored rectangles represent various cis-elements, with their positions indicated according to their locations within the promoters.</p>
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<p>The tissue expression pattern of <span class="html-italic">BrNAC</span> genes in different tissues including root, stem, leaf and inflorescence. Red indicates high expression levels, and blue indicates low expression levels.</p>
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<p>Expression profiles of <span class="html-italic">BrNAC2</span> genes in different tissues and organs. (<b>A</b>) Expression levels of <span class="html-italic">BrNAC2a</span>; (<b>B</b>) Expression levels of <span class="html-italic">BrNAC2b</span>; (<b>C</b>) Expression levels of <span class="html-italic">BrNAC2c.</span> Different letters represent significant difference (<span class="html-italic">p</span> &lt; 0.05).</p>
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<p>Subcellular localization of the BrNAC2s-eGFP in <span class="html-italic">Nicotiana benthamiana</span> leaves. pFGC5941-35S-eGFP and pFGC5941-35S-<span class="html-italic">BrNAC2s</span>-eGFP fusion proteins were transiently expressed in <span class="html-italic">N. tabacum</span> leaves. The fields included green fluorescence filed (488 nm), nucleus autofluorescence field (570 nm), bright field, and merged filed. Empty vector control showing the expression of 35S-eGFP in epidermal cells of <span class="html-italic">N. tabacum</span>, and co-localization of 35S-eGFP with BrNAC2 proteins observed by nucleus autofluorescence. Bars = 30 µm.</p>
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<p>Pro<span class="html-italic">BrNAC2s</span>–GUS expression in transgenic <span class="html-italic">A. thaliana</span>. (<b>A</b>) Expression activity of <span class="html-italic">BrNAC2a</span> promoter in inflorescence. (<b>B</b>) Expression activity of <span class="html-italic">BrNAC2b</span> promoter in inflorescence. (<b>C</b>) Expression activity of <span class="html-italic">BrNAC2c</span> promoter in inflorescence. Scale bars = 1 mm.</p>
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25 pages, 11574 KiB  
Article
Research on GRU-LSTM-Based Early Warning Method for Electric Vehicle Lithium-Ion Battery Voltage Fault Classification
by Liang Zhang, Qizhi Wu, Longfei Wang, Ling Lyu, Linru Jiang and Yu Shi
Energies 2025, 18(6), 1315; https://doi.org/10.3390/en18061315 - 7 Mar 2025
Viewed by 100
Abstract
Battery cell voltage is an important evaluation index for electric vehicle condition estimation and one of the main monitoring parameters of the battery management system, and accurate voltage prediction is crucial for electric vehicle battery failure warning. Therefore, this paper proposes a novel [...] Read more.
Battery cell voltage is an important evaluation index for electric vehicle condition estimation and one of the main monitoring parameters of the battery management system, and accurate voltage prediction is crucial for electric vehicle battery failure warning. Therefore, this paper proposes a novel hybrid gated recurrent unit and long short-term memory (GRU-LSTM) neural network to predict electric vehicle lithium-ion battery cell voltage. Firstly, Pearson coefficient correlation analysis is carried out to determine the input parameters of the neural network by analyzing the influence factors of the voltage parameters, and the hyperparameters of the neural network are determined through cross-validation to construct the lithium-ion battery single-unit voltage prediction model based on GRU-LSTM. Secondly, the voltage prediction accuracy and robustness of the GRU-LSTM model are verified by training the historical data of real vehicles in spring, summer, fall, and winter, combined with four different error indicators. Finally, the feasibility of the proposed method is verified by designing hierarchical warning rules based on the prediction data to realize the accurate warning of multiple voltage anomalies. Full article
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<p>Electric vehicle battery voltage fault warning flow chart.</p>
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<p>LSTM network architecture unit.</p>
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<p>GRU network architecture.</p>
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<p>GRU-LSTM hybrid model structure.</p>
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<p>Flowchart of the GRU-LSTM prediction model.</p>
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<p>Electric vehicle battery-related parameter curve.</p>
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<p>Pearson correlation coefficient between influencing factors.</p>
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<p>Training under different hidden layer nodes and iteration times.</p>
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<p>Training of different batch sizes at different learning rates.</p>
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<p>Battery voltage fitting during driving and charging periods.</p>
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<p>Prediction results and absolute errors of three algorithms.</p>
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<p>The R<sup>2</sup> values of the results predicted by the three algorithms.</p>
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<p>Battery voltage prediction effect in different seasons.</p>
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<p>Absolute value of battery voltage prediction error for different seasons.</p>
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<p>Battery voltage prediction error metrics for four seasons.</p>
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<p>The real voltage and predicted voltage curve of vehicle battery are studied.</p>
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<p>Change in voltage difference in a single battery.</p>
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<p>Standard deviation comparison between the true value and the predicted value.</p>
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<p>Dynamic early-warning effect of maximum/minimum cell voltage.</p>
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15 pages, 2416 KiB  
Article
Research on Self-Diagnosis and Self-Healing Technologies for Intelligent Fiber Optic Sensing Networks
by Ruiqi Zhang, Liang Fan and Dongzhu Lu
Sensors 2025, 25(6), 1641; https://doi.org/10.3390/s25061641 - 7 Mar 2025
Viewed by 132
Abstract
To address the issue of insufficient reliability of fiber optic sensing networks in complex environments, this study proposes a self-diagnosis and self-healing method based on intelligent algorithms. This method integrates redundant fiber paths and a fault detection mechanism, enabling rapid data transmission recovery [...] Read more.
To address the issue of insufficient reliability of fiber optic sensing networks in complex environments, this study proposes a self-diagnosis and self-healing method based on intelligent algorithms. This method integrates redundant fiber paths and a fault detection mechanism, enabling rapid data transmission recovery through redundant paths during network faults, ensuring the stable operation of the monitoring system. Unlike traditional self-diagnosis techniques that rely on an optical time domain reflectometer, the proposed self-diagnosis algorithm utilizes data structure analysis, significantly reducing dependence on costly equipment and improving self-diagnosis efficiency. On the hardware front, a light switch driving device that does not require an external power source has been developed, expanding the application scenarios of optical switches and enhancing system adaptability and ease of operation. In the experiments, three fiber optic sensing network topologies—redundant ring structure, redundant dual-ring structure, and redundant mesh structure—are constructed for testing. The results show that the average self-diagnosis time is 0.1257 s, and the self-healing time is 0.5364 s, validating the efficiency and practicality of the proposed method. Furthermore, this study also proposes a robustness evaluation model based on sensor perception ability and coverage uniformity indicators, providing a theoretical basis for the self-healing capability of fiber optic sensing networks. This model aids in network topology optimization and fault recovery strategy design, contributing to the improvement of the stability and reliability of fiber optic sensing networks in practical applications. Full article
(This article belongs to the Special Issue Advances in Optical Fiber Sensors and Fiber Lasers)
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<p>(<b>a</b>) Optical switch drive device and installation diagram; (<b>b</b>) schematic diagram of the actual network topology connection architecture.</p>
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<p>Algorithm flowchart.</p>
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<p>Schematic diagram of redundant topology structure: (<b>a</b>) redundant ring, (<b>b</b>) redundant dual-ring, (<b>c</b>) redundant mesh topology.</p>
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<p>Self-diagnostic and self-healing time for three redundant networks: (<b>a</b>) redundant ring; (<b>b</b>) redundant dual-ring; (<b>c</b>) redundant mesh topology.</p>
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<p>(<b>a</b>) Schematic diagram of optical switch combinations; (<b>b</b>) repair path under extreme damage.</p>
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<p>Robustness values of the three redundant networks and their non-redundant counterparts.</p>
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18 pages, 10057 KiB  
Article
Effects of NatureKnit™, a Blend of Fruit and Vegetable Fibers Rich in Naturally Occurring Bound Polyphenols, on the Metabolic Activity and Community Composition of the Human Gut Microbiome Using the M-SHIME® Gastrointestinal Model
by Marlies Govaert, Cindy Duysburgh, Brendan Kesler and Massimo Marzorati
Microorganisms 2025, 13(3), 613; https://doi.org/10.3390/microorganisms13030613 (registering DOI) - 7 Mar 2025
Viewed by 42
Abstract
This study evaluated the impact of a proprietary blend of fruit and vegetable fibers rich in naturally occurring bound polyphenols (commercially marketed as NatureKnitTM), compared to purified fibers (inulin and psyllium), on the human gut microbiome using the validated M-SHIME® [...] Read more.
This study evaluated the impact of a proprietary blend of fruit and vegetable fibers rich in naturally occurring bound polyphenols (commercially marketed as NatureKnitTM), compared to purified fibers (inulin and psyllium), on the human gut microbiome using the validated M-SHIME® gastrointestinal model. A short-term single-stage colonic M-SHIME® experiment (with fecal inoculum from three healthy human donors) was used to evaluate the test products compared to a negative control. Samples were assessed for pH, gas pressure, short-chain fatty acid (SCFA) production, lactate, and ammonium from 0 h to 48 h. Microbial community composition was assessed at 0 h (negative control only), 24 h, and 48 h (lumen) or 48 h (mucosal). All test products were fermented well in the colon as demonstrated by decreases in pH and increases in gas pressure over time; these changes occurred faster with the purified fibers, whereas NatureKnit™ demonstrated slow, steady changes, potentially indicating a gentler fermentation process. SCFA production significantly increased over the course of the 48 h experiment with all test products versus negative control. SCFA production was significantly greater with NatureKnit™ versus the purified fibers. Shifts in the microbial community composition were observed with all test products versus negative control. At the conclusion of the 48 h experiment, the absolute bacterial abundance and the richness of observed bacterial taxa in the lumen compartment was significantly greater with NatureKnit™ compared with inulin, psyllium, and negative control. Overall, NatureKnit™ demonstrated greater or similar prebiotic effects on study measures compared with established prebiotic fibers. Full article
(This article belongs to the Section Gut Microbiota)
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<p>Changes in (<b>a</b>) pH and (<b>b</b>) gas pressure over time following test product administration in M-SHIME<sup>®</sup> short-term colonic incubations. Incubations included the negative control (colonic incubation blank medium), NatureKnit™ (1.667 g fiber/L, 3.333 g/L total), inulin (1.667 g fiber/L), and psyllium (1.667 g fiber/L). Donors A, B, and C represent three individual healthy human fecal donors, and average donor represents the average of the three donors. Incubations were performed in triplicate (<span class="html-italic">n</span> = 3) and the results are presented as mean ± standard deviation. Statistical analysis was performed over the course of the entire colonic incubation phase (i.e., between 0 h and 48 h). Paired student’s <span class="html-italic">t</span>-tests were used to compare changes observed for the test products versus negative control. A <span class="html-italic">p</span>-value of &lt;0.05 was considered statistically significant. Different letters above the bars indicate statistically significant differences between test conditions, while no significant differences were observed between test conditions that share the same letter. M-SHIME<sup>®</sup> = Mucosal Simulator of the Human Intestinal Microbial Ecosystem.</p>
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<p>Changes in (<b>a</b>) total SCFA, (<b>b</b>) lactate, (<b>c</b>) branched SCFA, and (<b>d</b>) ammonium-N over time following test product administration in M-SHIME<sup>®</sup> short-term colonic incubations. Incubations included the negative control (colonic incubation blank medium), NatureKnit™ (1.667 g fiber/L, 3.333 g/L total), inulin (1.667 g fiber/L), and psyllium (1.667 g fiber/L). Donors A, B, and C represent three individual healthy human fecal donors, and average donor represents the average of the three donors. Incubations were performed in triplicate (<span class="html-italic">n</span> = 3) and the results are presented as mean ± standard deviation. Statistical analysis was performed over the course of the entire colonic incubation phase (i.e., between 0 h and 48 h) or over the course of the initial time interval (i.e., between 0 h and 6 h; lactate only). Paired student’s <span class="html-italic">t</span>-tests were used to compare changes observed for the test products versus negative control. A <span class="html-italic">p</span>-value of &lt;0.05 was considered statistically significant. Different letters above the bars indicate statistically significant differences between test conditions, while no significant differences were observed between test conditions that share the same letter. M-SHIME<sup>®</sup> = Mucosal Simulator of the Human Intestinal Microbial Ecosystem; SCFA = short chain fatty acid.</p>
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<p>Changes in (<b>a</b>) total SCFA, (<b>b</b>) lactate, (<b>c</b>) branched SCFA, and (<b>d</b>) ammonium-N over time following test product administration in M-SHIME<sup>®</sup> short-term colonic incubations. Incubations included the negative control (colonic incubation blank medium), NatureKnit™ (1.667 g fiber/L, 3.333 g/L total), inulin (1.667 g fiber/L), and psyllium (1.667 g fiber/L). Donors A, B, and C represent three individual healthy human fecal donors, and average donor represents the average of the three donors. Incubations were performed in triplicate (<span class="html-italic">n</span> = 3) and the results are presented as mean ± standard deviation. Statistical analysis was performed over the course of the entire colonic incubation phase (i.e., between 0 h and 48 h) or over the course of the initial time interval (i.e., between 0 h and 6 h; lactate only). Paired student’s <span class="html-italic">t</span>-tests were used to compare changes observed for the test products versus negative control. A <span class="html-italic">p</span>-value of &lt;0.05 was considered statistically significant. Different letters above the bars indicate statistically significant differences between test conditions, while no significant differences were observed between test conditions that share the same letter. M-SHIME<sup>®</sup> = Mucosal Simulator of the Human Intestinal Microbial Ecosystem; SCFA = short chain fatty acid.</p>
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<p>Stacked bar plots showing (<b>a</b>) absolute phyla abundances (cells/mL) in the lumen compartment and (<b>b</b>) relative phyla abundances in the mucosal compartment. Incubations included the negative control (colonic incubation blank medium), NatureKnit™ (1.667 g fiber/L, 3.333 g/L total), inulin (1.667 g fiber/L), and psyllium (1.667 g fiber/L). Donors A, B, and C represent three individual healthy human fecal donors. Incubations were performed in triplicate (<span class="html-italic">n</span> = 3). Flow cytometry was used to determine the total number of bacterial cells in the luminal samples.</p>
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<p>Jitter plots showing (<b>a</b>) the absolute abundance (cells/mL) of the top 20 most abundant phyla and families in the lumen compartment after 24 h, (<b>b</b>) the absolute abundance (cells/mL) of the top 20 most abundant phyla and families in the lumen compartment after 48 h, and (<b>c</b>) the relative abundance of the top 20 most abundant phyla and families in the mucosal compartment after 48 h. Incubations included the negative control (colonic incubation blank medium), NatureKnit™ (1.667 g fiber/L, 3.333 g/L total), inulin (1.667 g fiber/L), and psyllium (1.667 g fiber/L). Incubations were performed for each donor in triplicate (per donor, <span class="html-italic">n</span> = 3; total, <span class="html-italic">n</span> = 9). Each dot represents the average across donors.</p>
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<p>Effect of the test products on alpha diversity as calculated by the observed taxa, Shannon, and inverse Simpson indexes in the (<b>a</b>) lumen compartment (24 h and 48 h) and (<b>b</b>) mucosal compartment (48 h). Incubations included the negative control (colonic incubation blank medium), NatureKnit™ (1.667 g fiber/L, 3.333 g/L total), inulin (1.667 g fiber/L), and psyllium (1.667 g fiber/L). Donors A, B, and C represent three individual healthy human fecal donors. Incubations were performed in triplicate (<span class="html-italic">n</span> = 3). Paired student’s <span class="html-italic">t</span>-tests were used to compare each test product with the negative control. A <span class="html-italic">p</span>-value of &lt;0.05 was considered statistically significant; the asterisk represents a significant difference versus negative control.</p>
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29 pages, 10427 KiB  
Article
Cultural Perception of Tourism Heritage Landscapes via Multi-Label Deep Learning: A Study of Jingdezhen, the Porcelain Capital
by Yue Cheng and Weizhen Chen
Land 2025, 14(3), 559; https://doi.org/10.3390/land14030559 - 6 Mar 2025
Viewed by 86
Abstract
In the face of rapid progress in heritage preservation and cultural tourism integration, landscape planning in historic cities is pivotal to showcasing regional identities and disseminating cultural value. However, the complexity of cultural characteristic identification and the imbalance in planning often restrict the [...] Read more.
In the face of rapid progress in heritage preservation and cultural tourism integration, landscape planning in historic cities is pivotal to showcasing regional identities and disseminating cultural value. However, the complexity of cultural characteristic identification and the imbalance in planning often restrict the progress of urban development. Additionally, existing studies predominantly rely on subjective methods and focus on a single cultural attribute, highlighting the urgent need for research on diversified cultural perception. Using Jingdezhen, a renowned historic cultural city, as an example, this study introduces a multi-label deep learning approach to examine cultural perceptions in tourism heritage landscapes. Leveraging social media big data and an optimized ResNet-50 model, a framework encompassing artifacts, production, folk, and living culture was constructed and integrated with ArcGIS spatial analysis and diversity indices. The results show: (1) The multi-label classification model achieves 92.35% accuracy, validating its potential; (2) Heritage landscapes exhibit a “material-dominated, intangible-weak” structure, with artifacts culture as the main component; (3) Cultural perception intensity is unevenly distributed, with core areas demonstrating higher recognition and diversity; (4) Diversity indices suggest that comprehensive venues display stronger cultural balance, whereas specialized ones reveal marked cultural singularity, indicating a need for improved integration across sites. This research expands the use of multi-label deep learning in tourism heritage studies and offers practical guidance for global heritage sites tackling mass tourism. Full article
(This article belongs to the Special Issue Landscape Planning for Mass Tourism in Historical Cities)
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<p>Research framework for multi-label cultural perception of CCHL.</p>
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<p>Overview of the Jingdezhen region.</p>
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<p>ResNet50 architecture: detailed structure of ResNet50 stages and residual blocks.</p>
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<p>Enhanced ResNet-50 architecture. In the diagram, the circular symbols represent addition and multiplication.</p>
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<p>The interface of dataset annotation using the labellmg program.</p>
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<p>Expert sampling evaluation of venue images. The bar graph displays consensus scores for each image, with the overall mean consensus score (3.72) indicated by a red dashed line.</p>
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<p>Model performance results for different hyperparameters.</p>
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<p>Several results from multi-label classification using the ResNet-50 model demonstrate high prediction accuracy for CCHL.</p>
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<p>The percentage distribution of four types of cultural patterns in CCHL.</p>
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<p>Cultural perception results of eleven CCHL in Jingdezhen.</p>
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<p>Absolute and relative quantities of cultural perception in different CCHL. a1–a4 represent the absolute number of four cultures (artifacts culture, production culture, folk culture, and living culture), and b1–b4 represent the relative number of four cultures.</p>
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<p>Shannon diversity index and Simpson index distribution of the CCHL.</p>
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25 pages, 17447 KiB  
Article
BuZhong YiQi Formula Alleviates Diabetes-Caused Hyposalivation by Activating Salivary Secretion Pathway in the Parotid and Submandibular Glands of Rats
by Ming-Yu Wang, Zhen-Ran Hu, Liang Wang, Xin-Xin Zeng, Xiang-Ke Li, Guo-Jun Fei, Jing-Li Zhang, Jing-Ru Chen and Ze-Min Yang
Pharmaceuticals 2025, 18(3), 377; https://doi.org/10.3390/ph18030377 - 6 Mar 2025
Viewed by 77
Abstract
Background/Objectives: BuZhong Yiqi Formula (BZYQF) has significant ameliorative effects on type 2 diabetes mellitus (T2DM). However, its efficacy in alleviating the hyposalivation caused by T2DM needs to be confirmed, and its mechanism is unclear. Methods: Network pharmacology and molecular docking were [...] Read more.
Background/Objectives: BuZhong Yiqi Formula (BZYQF) has significant ameliorative effects on type 2 diabetes mellitus (T2DM). However, its efficacy in alleviating the hyposalivation caused by T2DM needs to be confirmed, and its mechanism is unclear. Methods: Network pharmacology and molecular docking were combined to analyze the molecular mechanism by which BZYQF alleviates T2DM-caused hyposalivation. A T2DM rat model was induced to evaluate the efficacy of BZYQF. The total saliva before and after acid stimulation was collected to determine the salivary flow rate and salivary alpha-amylase (sAA) activity. The parotid (PG) and submandibular glands (SMG) of experimental rats were removed to perform histopathology observation, biochemical indicator determination, and expression detection of signaling molecules in the salivary secretion pathway. Results: The present study screened out 1014 potential targets of BZYQF regarding the treatment of T2DM. These targets were mainly involved in the formation of the receptor complex, exercising the neurotransmitter receptor activity and regulating secretion. They were significantly enriched in the salivary secretion pathway of β1-AR/PKA/AMY1 and CHRM3/IP3R/AQP5. Furthermore, in BZYQF, nine validated compounds were able to dock into the active site of β1-AR, and three validated compounds were able to dock into the active site of CHRM3. Animal experiments confirmed that BZYQF significantly reduces fasting blood glucose, total cholesterol and triglyceride levels; enhances insulin level and HOMA-IS (p < 0.05); and increases salivary flow rate (Basal: increase from 21.04 ± 14.31 to 42.65 ± 8.84 μL/min, effect size of Cohen’s d = 6.80, p = 0.0078; Stimulated: increase from 36.88 ± 17.48 to 72.63 ± 17.67 μL/min, effect size of Cohen’s d = 7.61, p = 0.0025) and sAA activity (Basal: increase from 0.68 ± 0.32 to 2.17 ± 0.77 U/mL, effect size of Cohen’s d = 9.49, p = 0.0027; Stimulated: increase from 1.15 ± 0.77 to 4.80 ± 1.26 U/mL, effect size of Cohen’s d = 13.10, p = 0.0001) in basal and stimulated saliva in T2DM rats. Further mechanistic studies revealed that BZYQF reduces glucose and lipid accumulation, enhances acetylcholine content, improves pathological lesions and inflammation, and significantly increases the expression of salivary secretion pathway signaling molecules, including PKA, IP3R, β1-AR, AQP5, CHRM3, and AMY1 in the PG and SMG of T2DM rats (p < 0.05). Conclusions: The present study demonstrated that BZYQF is able to alleviate T2DM-caused hyposalivation by improving glucose metabolism and activating the salivary secretion pathway in the PG and SMG of T2DM rats. This study might provide a novel rationale and treatment strategy for BZYQF in diabetes-induced hyposalivation in a clinical setting. Full article
(This article belongs to the Section Natural Products)
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<p>The enriched GO terms and KEGG pathways by the common targets of drug and disease and compound-target-salivary secretion pathway interaction network. The orange underline indicates the enriched GO-BP, MF, CC, and KEGG related to saliva secretion. The orange squares indicates the top 10 genes with the highest number of nodes in the compound-target-salivary secretion interaction network.</p>
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<p>The docked 3D and 2D diagrams based on predicted optimal binding mode of validated compounds in BZYQF to β1-AR and CHRM3. They indicate CDOCKER interaction energy with a unit of Kcal/mol. Isoproterenol and iperoxo are agonists of β1-AR and CHRM3, respectively. Pilocarpine is a sialogogue drug working on muscarinic receptors.</p>
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<p>Daily indicators of experimental rats. CON group: N = 8; T2DM group: N = 8; BZYQF group, N = 8; MH group, N = 6.</p>
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<p>Basal and stimulated salivary parameters in experimental rats. CON group: N = 8; T2DM group: N = 8; BZYQF group, N = 8; MH group, N = 6.</p>
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<p>HE staining of PG tissue in experimental rats (400×). Black arrows indicate acinar cells, yellow arrows indicate inflammatory cells, green arrows indicate secretory ducts, and red arrows indicate interlobular ducts. CON group: N = 3; T2DM group: N = 3; BZYQF group, N = 3.</p>
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<p>HE staining of SMG tissue in experimental rats (400×). Blue arrows indicate serous acinar cells, black arrows indicate mucinous acinar cells, yellow arrows indicate inflammatory cells, and green arrows indicate secretory ducts. CON group: N = 3; T2DM group: N = 3; BZYQF group, N = 3.</p>
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<p>IHC staining for sAA in the PG and SMG tissue of experimental rats. (<b>a</b>–<b>c</b>) PG; (<b>d</b>–<b>f</b>) SMG. Magnification is 40× and 100×. CON group: N = 3; T2DM group: N = 3; BZYQF group, N = 3.</p>
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<p>The mRNA expression of inflammatory factors and salivary secretion pathway signaling molecules in the PG of experimental rats. CON group: N = 6–8; T2DM group: N = 6–8; BZYQF group, N = 6–8.</p>
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<p>The mRNA expression of inflammatory factors and salivary secretion pathway signaling molecules in the SMG of experimental rats. CON group: N = 6–8; T2DM group: N = 6–8; BZYQF group, N = 6–8.</p>
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<p>The protein expression of β1-AR, sAA and CHRM3 in the PG and SMG of experimental rats. CON group: N = 3; T2DM group: N = 3; BZYQF group, N = 3.</p>
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27 pages, 10829 KiB  
Article
Potentiality Delineation of Groundwater Recharge in Arid Regions Using Multi-Criteria Analysis
by Heba El-Bagoury, Mahmoud H. Darwish, Sedky H. A. Hassan, Sang-Eun Oh, Kotb A. Attia and Hanaa A. Megahed
Water 2025, 17(5), 766; https://doi.org/10.3390/w17050766 - 6 Mar 2025
Viewed by 119
Abstract
This study integrates morphometric analysis, remote sensing, and GIS with the analytical hierarchical process (AHP) to identify high potential groundwater recharge areas in Wadi Abadi, Egyptian Eastern Desert, supporting sustainable water resource management. Groundwater recharge primarily comes from rainfall and Nile River water, [...] Read more.
This study integrates morphometric analysis, remote sensing, and GIS with the analytical hierarchical process (AHP) to identify high potential groundwater recharge areas in Wadi Abadi, Egyptian Eastern Desert, supporting sustainable water resource management. Groundwater recharge primarily comes from rainfall and Nile River water, particularly for Quaternary aquifers. The analysis focused on the Quaternary and Nubian Sandstone aquifers, evaluating 16 influencing parameters, including elevation, slope, rainfall, lithology, soil type, and land use/land cover (LULC). The drainage network was derived from a 30 m-resolution Digital Elevation Model (DEM). ArcGIS 10.8 was used to classify the basin into 13 sub-basins, with layers reclassified and weighted using a raster calculator. The groundwater potential map revealed that 24.95% and 29.87% of the area fall into very low and moderate potential categories, respectively, while low, high, and very high potential zones account for 18.62%, 17.65%, and 8.91%. Data from 41 observation wells were used to verify the potential groundwater resources. In this study, the ROC curve was applied to assess the accuracy of the GWPZ models generated through different methods. The validation results indicated that approximately 87% of the wells corresponded accurately with the designated zones on the GWPZ map, confirming its reliability. Over-pumping in the southwest has significantly lowered water levels in the Quaternary aquifer. This study provides a systematic approach for identifying groundwater recharge zones, offering insights that can support resource allocation, well placement, and aquifer sustainability in arid regions. This study also underscores the importance of recharge assessment for shallow aquifers, even in hyper-arid environments. Full article
(This article belongs to the Special Issue Advance in Groundwater in Arid Areas)
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<p>(<b>a</b>) Egypt Landsat satellite image; (<b>b</b>) study area by Google Earth image, 2024.</p>
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<p>Geological map of Wadi Abadi Basin (after CONCO, 1987 [<a href="#B63-water-17-00766" class="html-bibr">63</a>]).</p>
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<p>The flowchart of approaches and methodology.</p>
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<p>Geographical distribution of the main groundwater aquifers and forty-two of drilled wells of the Wadi Abadi basin.</p>
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<p>Hydrogeological cross-section (A–A′) of the Nubia Sandstone aquifer at the study area.</p>
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<p>(<b>a</b>) Digital elevation model; (<b>b</b>) slope; (<b>c</b>) aspect; (<b>d</b>) rainfall distribution; (<b>e</b>) lithology; (<b>f</b>) soil types; and (<b>g</b>) LULC.</p>
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<p>(<b>a</b>) Stream order and number; (<b>b</b>) stream length; (<b>c</b>) bifurcation ratio; (<b>d</b>) drainage density; (<b>e</b>) length of overland flow; (<b>f</b>) stream frequency; (<b>g</b>) drainage texture; (<b>h</b>) elongation ratio; and (<b>i</b>) relief ratio.</p>
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<p>A groundwater potential zone map (GWPZ) associated with observation wells illustrating the classes of potential recharge zoning at Wadi Abadi.</p>
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29 pages, 3120 KiB  
Article
Linear Model and Gradient Feature Elimination Algorithm Based on Seasonal Decomposition for Time Series Forecasting
by Sheng-Tzong Cheng, Ya-Jin Lyu and Yi-Hong Lin
Mathematics 2025, 13(5), 883; https://doi.org/10.3390/math13050883 - 6 Mar 2025
Viewed by 159
Abstract
In the wave of digital transformation and Industry 4.0, accurate time series forecasting has become critical across industries such as manufacturing, energy, and finance. However, while deep learning models offer high predictive accuracy, their lack of interpretability often undermines decision-makers’ trust. This study [...] Read more.
In the wave of digital transformation and Industry 4.0, accurate time series forecasting has become critical across industries such as manufacturing, energy, and finance. However, while deep learning models offer high predictive accuracy, their lack of interpretability often undermines decision-makers’ trust. This study proposes a linear time series model architecture based on seasonal decomposition. The model effectively captures trends and seasonality using an additive decomposition, chosen based on initial data visualization, indicating stable seasonal variations. An augmented feature generator is introduced to enhance predictive performance by generating features such as differences, rolling statistics, and moving averages. Furthermore, we propose a gradient-based feature importance method to improve interpretability and implement a gradient feature elimination algorithm to reduce noise and enhance model accuracy. The approach is validated on multiple datasets, including order demand, energy load, and solar radiation, demonstrating its applicability to diverse time series forecasting tasks. Full article
20 pages, 6179 KiB  
Article
Non-Contact Dimensional Quality Inspection System of Prefabricated Components Using 3D Matrix Camera
by Wanqing Lyu, Xiwang Chen, Wenlong Han, Kun Ni, Rui Jing, Lin Tong, Junzheng Pan and Qian Wang
Buildings 2025, 15(5), 837; https://doi.org/10.3390/buildings15050837 (registering DOI) - 6 Mar 2025
Viewed by 102
Abstract
Dimensional quality inspection of prefabricated components is crucial for ensuring building quality and safety. Currently, manual measurement methods are predominantly used in dimensional quality inspection of prefabricated components, which are both time-consuming and labor-intensive, constraining production efficiency. This study thus developed a non-contact [...] Read more.
Dimensional quality inspection of prefabricated components is crucial for ensuring building quality and safety. Currently, manual measurement methods are predominantly used in dimensional quality inspection of prefabricated components, which are both time-consuming and labor-intensive, constraining production efficiency. This study thus developed a non-contact image measurement system using an innovative three-dimensional (3D) matrix camera, which automatically performed dimensional quality inspection, utilizing technologies such as a parallel optical axis four-camera matrix imaging and machine learning algorithms. Compared to traditional techniques, this system exhibited enhanced adaptability to the manufacturing process of prefabricated components, along with desirable accuracy and efficiency. Building upon a comprehensive literature review, the hardware constituents of the 3D matrix camera image measurement system were meticulously introduced, followed by the underlying principles and implementations of data acquisition, processing and comparison methods, including parallel optical axis four-camera matrix imaging, automatic stitching algorithms for 3D point clouds, feature recognition algorithms, and matching principles. The feasibility of the proposed system was validated through a case study analysis. The application results indicated that the system was capable of automatically performing non-contact measurements of dimensional deviations in prefabricated components with an accuracy of ±3 mm, thereby enhancing production quality. Full article
(This article belongs to the Special Issue Intelligence and Automation in Construction Industry)
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<p>Manual measurement inspection of prefabricated component dimensions.</p>
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<p>Hardware composition of 3D matrix camera image measurement system.</p>
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<p>Schematic diagrams of single set of matrix cameras and its field of view.</p>
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<p>Schematic layout of matrix camera array.</p>
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<p>Software system of 3D matrix camera image measurement system.</p>
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<p>Schematic diagram of imaging principle.</p>
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<p>Generalized steps and algorithms for matching operations.</p>
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<p>Placement rules of prefabricated components on molding table.</p>
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<p>Photos of application case.</p>
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<p>Three-dimensional point clouds generated by one set of matrix cameras.</p>
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<p>Three-dimensional point cloud stitching.</p>
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<p>Three-dimensional point clouds of one prefabricated component, obtained through segmentation.</p>
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<p>Local recognition results of corresponding features.</p>
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<p>Visualization of measurement results.</p>
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