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17 pages, 3279 KiB  
Article
TCN-GRU Based on Attention Mechanism for Solar Irradiance Prediction
by Zhi Rao, Zaimin Yang, Xiongping Yang, Jiaming Li, Wenchuan Meng and Zhichu Wei
Energies 2024, 17(22), 5767; https://doi.org/10.3390/en17225767 (registering DOI) - 18 Nov 2024
Abstract
The global horizontal irradiance (GHI) is the most important metric for evaluating solar resources. The accurate prediction of GHI is of great significance for effectively assessing solar energy resources and selecting photovoltaic power stations. Considering the time series nature of the GHI and [...] Read more.
The global horizontal irradiance (GHI) is the most important metric for evaluating solar resources. The accurate prediction of GHI is of great significance for effectively assessing solar energy resources and selecting photovoltaic power stations. Considering the time series nature of the GHI and monitoring sites dispersed over different latitudes, longitudes, and altitudes, this study proposes a model combining deep neural networks and deep convolutional neural networks for the multi-step prediction of GHI. The model utilizes parallel temporal convolutional networks and gate recurrent unit attention for the prediction, and the final prediction result is obtained by multilayer perceptron. The results show that, compared to the second-ranked algorithm, the proposed model improves the evaluation metrics of mean absolute error, mean absolute percentage error, and root mean square error by 24.4%, 33.33%, and 24.3%, respectively. Full article
(This article belongs to the Section A2: Solar Energy and Photovoltaic Systems)
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Figure 1

Figure 1
<p>The framework diagram of TCN.</p>
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<p>The framework diagram of causal convolution.</p>
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<p>The framework diagram of dilated convolution.</p>
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<p>The framework diagram of residual connection.</p>
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<p>The framework diagram of GRU.</p>
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<p>The framework diagram of attention mechanism.</p>
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<p>The framework diagram of MLP.</p>
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<p>The framework diagram of TGAM.</p>
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<p>The prediction fitting plot of TGAM.</p>
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<p>The prediction fitting plot of TGAM and comparison algorithms.</p>
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<p>The evaluation indicators and improvement percentages of TGAM and comparison algorithms: (<b>a</b>) MAE; (<b>b</b>) MAPE; (<b>c</b>) RMSE.</p>
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<p>The impact of missing variables on model prediction: (<b>a</b>) MAE; (<b>b</b>) MAPE; (<b>c</b>) RMSE.</p>
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14 pages, 2175 KiB  
Article
Optimizing Basil Seed Vigor Evaluations: An Automatic Approach Using Computer Vision-Based Technique
by Júlio César Altizani-Júnior, Silvio Moure Cicero, Cristina Batista de Lima, Rafael Mateus Alves and Francisco Guilhien Gomes-Junior
Horticulturae 2024, 10(11), 1220; https://doi.org/10.3390/horticulturae10111220 - 18 Nov 2024
Abstract
The short cultivation cycle and high essential oil content of basil plants render them a valuable raw material source for diverse industries. However, large-scale production is hindered by the lack of specific protocols to assess seed vigor; thus, a consistent supply of high-quality [...] Read more.
The short cultivation cycle and high essential oil content of basil plants render them a valuable raw material source for diverse industries. However, large-scale production is hindered by the lack of specific protocols to assess seed vigor; thus, a consistent supply of high-quality seeds that meet consumer demands cannot be ensured. This study investigated the effectiveness of an automated system for seedling analysis as a tool for evaluating basil seed vigor and compared it to traditional tests. For this purpose, seeds from eight commercial lots were evaluated in two separate trials spaced six months apart using the following tests: germination, first germination count, saturated salt accelerated aging, primary root emergence, mean germination time, seedling emergence, seedling emergence speed index, and computerized seedling image analysis. The parameters provided by the system allowed us to clearly and objectively classify the basil seed lots based on vigor, and the results were strongly and significantly correlated with the findings of traditional vigor tests, particularly between the vigor index and seedling length. Digital analysis of four-day-old seedlings proved to be a fast and efficient technique for evaluating basil seed vigor and has the potential for use in automating the data collection and analysis process. Full article
(This article belongs to the Section Propagation and Seeds)
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Figure 1
<p>Step-by-step procedure for automated image analysis of basil seedlings using SVIS<sup>®</sup> software. Seeds were sown on blotting paper at 2 cm (top row) and 6 cm (bottom row) from the top of the paper; after sowing, the containers were placed in a germination chamber at a 70° angle with the horizontal (<b>A</b>). Seedlings and non-germinated seeds were transferred to a blue ethylene vinyl acetate sheet and scanned to capture images (<b>B</b>). Captured images were processed to generate development uniformity, growth, and vigor indices. Analyzed seedlings are highlighted in red, while non-germinated seeds are marked in green (<b>C</b>).</p>
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<p>Primary root emergence progress curves for basil seed lots. Values represent the mean ± standard error (<span class="html-italic">n</span> = 8).</p>
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<p>Visual appearance of three- and four-day-old basil seedlings. The white line indicates the image scale (2.0 cm).</p>
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<p>Length of three- and four-day-old basil seedlings from seed lots subjected to computerized image analysis using the SVIS<sup>®</sup> software. The red crosses indicate the values used as a reference for calculating the growth index (3.05 and 5.08 cm). Values represent the mean (<span class="html-italic">n</span> = 200).</p>
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<p>Hypothetical scheme of the distribution of seed lots in the categories of high, medium, and low vigor as a function of the different phases of the deterioration process.</p>
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19 pages, 2508 KiB  
Review
Plant Responses and Adaptations to Salt Stress: A Review
by Cuiyu Liu, Xibing Jiang and Zhaohe Yuan
Horticulturae 2024, 10(11), 1221; https://doi.org/10.3390/horticulturae10111221 - 18 Nov 2024
Abstract
Salinity poses a significant environmental challenge, limiting plant growth and development. To cultivate salt-tolerant plants, it is crucial to understand the physiological, biochemical, and molecular responses and adaptations to salt stress, as well as to explore natural genetic resources linked to salt tolerance. [...] Read more.
Salinity poses a significant environmental challenge, limiting plant growth and development. To cultivate salt-tolerant plants, it is crucial to understand the physiological, biochemical, and molecular responses and adaptations to salt stress, as well as to explore natural genetic resources linked to salt tolerance. In this review, we provide a detailed overview of the mechanisms behind morphological and physiological responses triggered by salt stress, including salt damage to plants, the disturbance of cell osmotic potentials and ion homeostasis, lipid peroxidation, and the suppression of photosynthesis and growth. We also describe the physiological mechanisms that confer salt tolerance in plants, such as osmotic adjustments, reactive oxygen species (ROS) scavenging, photosynthetic responses, phytohormone regulation, and ion regulation. Additionally, we summarize the salt-stress sensing and signaling pathways, gene regulatory networks, as well as salt-tolerance mechanisms in plants. The key pathways involved in salt-stress signal perception and transduction, including Ca2+-dependent protein kinase (CDPK) cascades, the salt overly sensitive (SOS) pathway, and the abscisic acid (ABA) pathway, are discussed, along with relevant salt-stress-responsive genes and transcription factors. In the end, the important issues and challenges related to salt tolerance for future research are addressed. Overall, this review aims to provide essential insights for the future cultivation and breeding of crops and fruits. Full article
(This article belongs to the Section Biotic and Abiotic Stress)
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Figure 1

Figure 1
<p>Plant physiological and biochemical responses and adaptations to salt stress. ROS: reactive oxygen species, SOD: superoxide dismutase, POD: peroxidase, CAT: catalase, APX: ascorbate peroxidase, GR: glutathione reductase, AsA: ascorbic acid, GSH: glutathione, Car: carotenoids, GB: glycine betaine, ABA: abscisic acid, SA: salicylic acid, JA: jasmonic acid, IAA: auxin, GA: gibberellin, CK: cytokinin, BR: brassinosteroids, ET: ethylene, Chl a: chlorophyll a, Chl b: chlorophyll b, Pn: photosynthesis rate, gs: stomatal conductance, Tr: transpiration rate, Ci: CO<sub>2</sub> concentration, Fv/Fm: optimal/maximal photochemical efficiency of PSII in the dark.</p>
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<p>Ion uptake and transport in plant tissues under NaCl stress. ADP: adenosine diphosphate, AKT1: affinity K<sup>+</sup> transporter 1, ATP: adenosine triphosphate, CCC: cation-chloride cotransporter, CHX: Cation/H<sup>+</sup> exchanger, CLC: chloride channel, CNGC: cyclic nucleotide-gated channel, GLR: glutamate receptor-like channel, GR: glutathione reductase, GSH: glutathione, HKT1/HAK5: high-affinity K<sup>+</sup> transporter 1/5, NHX: Na<sup>+</sup>/H<sup>+</sup> antiporter, NSCC: non-selective cation channels, SLAH: slow anion channel-associated 1 homolog, SOS1: salt overly sensitive gene 1.</p>
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<p>The molecular responses of plants to salt stress. ABA: abscisic acid, AKT1: affinity K<sup>+</sup> transporter 1, AP2/ERF: Apetala2/ethylene response factor, APX: ascorbate peroxidase, AQP: aquaporin, AsA: ascorbic acid, bHLH: basic helix–loop–helix, bZIP: basic leucine zipper, CaM: calmodulin, CaMBP: CaM-like binding protein, CAT: catalase, CDPK: Ca<sup>2+</sup>-dependent protein kinase, CHX: Cation/H<sup>+</sup> exchanger, CLC: chloride channel, CML: CaM-like, CNGC: cyclic nucleotide-gated channel, DREB/CBF: dehydration-responsive element-binding protein/C-repeat binding factor, GLR: glutamate receptor-like protein, GR: glutathione reductase, GSH: glutathione, HKT1/HAK5: high-affinity K<sup>+</sup> transporter 1/5, HSP: heat shock protein, LEA: late embryogenesis abundant protein, MAPKs: mitogen-activated protein kinases, MAPKK: MAPK kinase, MAPKKK: MAPKK kinase, NHX: Na<sup>+</sup>/H<sup>+</sup> antiporter, NSCCs: non-selective cation channels, POD: peroxidase, PP2C: protein phosphatase 2C, PYR/PYL: pyrabactin resistance/pyrabactin resistance 1-like protein, SCaBPs: SOS3-like calcium-binding protein, SnRK2: sucrose non-fermenting-related protein kinase 2, SOD: superoxide dismutase, SOS1/2/3: salt overly sensitive gene 1/2/3.</p>
Full article ">Figure 4
<p>The mechanisms of plant responses and adaptations to salt stress. ABA: abscisic acid, AKT1: affinity K<sup>+</sup> transporter 1, AP2/ERF: Apetala2/ethylene response factor, APX: ascorbate peroxidase, AQP: aquaporin, AsA: ascorbic acid, bHLH: basic helix–loop–helix, BR: brassinosteroids, bZIP: basic leucine zipper, CAT: catalase, CAX: H<sup>+</sup>/Ca<sup>2+</sup> antiporter, CDPK: Ca<sup>2+</sup>-dependent protein kinase, Chl a: chlorophyll a, Chl b: chlorophyll b, Ci: CO<sub>2</sub> concentration, CK: cytokinin, CLC: chloride channel, DREB/CBF: dehydration-responsive element-binding protein/C-repeat binding factor, ET: ethylene, GA: gibberellin, GB: glycine betaine, GR: glutathione reductase, gs: stomatal conductance, GSH: glutathione, HKT1: high-affinity K<sup>+</sup> transporter 1, HSP: heat shock protein, IAA: auxin, JA: jasmonic acid, LEA: late embryogenesis abundant protein, MAPKs: mitogen-activated protein kinases, NHX1: Na<sup>+</sup>/H<sup>+</sup> antiporter 1, Pn: photosynthesis rate, POD: peroxidase, ROS: reactive oxygen species, SA: salicylic acid, SOD: superoxide dismutase, SOS: salt overly sensitive, Tr: transpiration rate.</p>
Full article ">
18 pages, 775 KiB  
Article
Valorization of Bioactive Compounds from Lingonberry Pomace and Grape Pomace with Antidiabetic Potential
by Elena Neagu, Gabriela Paun, Camelia Albu and Gabriel Lucian Radu
Molecules 2024, 29(22), 5443; https://doi.org/10.3390/molecules29225443 (registering DOI) - 18 Nov 2024
Abstract
In recent years, increased attention has been paid to the recovery of bioactive compounds from waste and by-products resulting from the agro-industrial sector and their valorization into new products, which can be used in the health, food, or agricultural industry, as innovative and [...] Read more.
In recent years, increased attention has been paid to the recovery of bioactive compounds from waste and by-products resulting from the agro-industrial sector and their valorization into new products, which can be used in the health, food, or agricultural industry, as innovative and sustainable approaches to waste management. In this work, two of these by-products resulting from the fruit-processing industry were used for the recovery of bioactive compounds (polyphenols), namely lingonberry pomace (Vaccinium vitis-idaea) and grape pomace (Vitis vinifera). Two green extraction techniques were employed to obtain hydroalcoholic extracts (solvent: 50% EtOH, 10% mass): ultrasound-assisted extraction (UAE) and accelerated solvent extraction (ASE). The extracts were subjected to micro- and ultrafiltration processes, and further analyzed to determine the bioactive compound content through spectrophotometric (UV-Vis) and chromatographic (HPLC-PDA) methods. Additionally, the extracts exhibited significant enzyme inhibition, particularly against α-amylase and β-glucosidase, suggesting potential anti-diabetic properties. The extracts characteristics, polyphenolic content, antioxidant capacity and enzyme inhibitory ability, were statistically compared, and significant differences were found between the two extraction methods. The grape pomace concentrated extracts showed a pronounced inhibitory activity on both analyzed enzymes compared to the lingonberry pomace concentrated extracts, closer to the standard used; e.g., IC50 α-amylase = 0.30 ± 0.01 µg/mL (IC50 acarbose = 0.3 ± 0.01 µg/mL), IC50 α-glucosidase = 0.60 ± 0.01 µg/mL (IC50 acarbose = 0.57 ± 0.02 µg/mL). These findings highlight the potential of agro-industrial residues as bioactive compound resources, with their valorization through application in food, nutraceutical, or pharmaceutical industries therefore contributing to the sustainable development and promotion of circular economy principles with the recovery of valuable inputs from plant by-products. Full article
20 pages, 8743 KiB  
Article
Effects of Heavy Grazing on Interspecific Relationships at Different Spatial Scales in Desert Steppe of China
by Xiaoyu Du, Jun Zhang, Juhong Liu, Shijie Lv and Haijun Liu
Sustainability 2024, 16(22), 10059; https://doi.org/10.3390/su162210059 - 18 Nov 2024
Abstract
This study investigates the effects of grazing intensity and spatial scale on the important values, interspecific relationships, and community stability of desert steppe plant communities in Siziwang Banner, Inner Mongolia, China. Using vegetation data collected at three spatial scales (50 m × 50 [...] Read more.
This study investigates the effects of grazing intensity and spatial scale on the important values, interspecific relationships, and community stability of desert steppe plant communities in Siziwang Banner, Inner Mongolia, China. Using vegetation data collected at three spatial scales (50 m × 50 m, 25 m × 25 m, and 2.5 m × 2.5 m) under two grazing conditions (no grazing and heavy grazing), we employed ecological statistics, including variance ratio analysis, χ2 tests, and the Jaccard index, to analyze species interactions and community structure. The results indicated that the important values of species vary with both spatial scale and grazing intensity; for example, Stipa breviflori and Chenopodium aristatum exhibited significantly higher important values in heavily grazed areas. Larger spatial scales enhanced the dominance of Cleistogenes songorica and Chenopodium aristatum, while smaller scales favored Stipa breviflori and Caragana stenophylla. Furthermore, interspecific associations were stronger in heavy grazing conditions. The community demonstrated consistent instability; however, no grazing areas were more stable than heavily grazed ones. These findings highlight that species importance, interspecific relationships, and community stability are closely linked to grazing intensity and spatial scale, emphasizing the critical role of sustainable grazing management in maintaining the long-term stability and resilience of desert steppe ecosystems. By emphasizing the need for targeted and sustainable management strategies, this study aims to contribute to the restoration and preservation of these vital ecosystems. Full article
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Figure 1
<p>The location of Siziwang Banner in Inner Mongolia and experiment plots in Siziwang Banner.</p>
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<p>The diagram of the spatial scale plot.</p>
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<p><math display="inline"><semantics> <mrow> <msup> <mrow> <mi>χ</mi> </mrow> <mrow> <mn>2</mn> </mrow> </msup> </mrow> </semantics></math> test and Jaccard index semi-matrix of population in 2.5 m × 2.5 m ungrazed area. The lower diagonal is the <math display="inline"><semantics> <mrow> <msup> <mrow> <mi>χ</mi> </mrow> <mrow> <mn>2</mn> </mrow> </msup> </mrow> </semantics></math> test, and the upper diagonal is the Jaccard index. 1: <span class="html-italic">Caragana stenophylla</span>, 2: <span class="html-italic">Stipa breviflora</span>, 3: <span class="html-italic">Cleistogenes songorica</span>, 4: <span class="html-italic">Aparagus lucidus lindl</span>, 5: <span class="html-italic">Convolvulus ammannii</span>, 6: <span class="html-italic">Lagochilus ilicifolium</span>, 7: <span class="html-italic">Chenopodium aristatum</span>, 8: <span class="html-italic">Allium tenuissimum</span>, 9: <span class="html-italic">Ceratoides latens</span>, 10: <span class="html-italic">Salsola collina</span>, and 11: <span class="html-italic">Kochia prostrata</span>.</p>
Full article ">Figure 4
<p><math display="inline"><semantics> <mrow> <msup> <mrow> <mi>χ</mi> </mrow> <mrow> <mn>2</mn> </mrow> </msup> </mrow> </semantics></math> test and Jaccard index semi-matrix of population in 2.5 m × 2.5 m heavily grazed area. The lower diagonal is the <math display="inline"><semantics> <mrow> <msup> <mrow> <mi>χ</mi> </mrow> <mrow> <mn>2</mn> </mrow> </msup> </mrow> </semantics></math> test, and the upper diagonal is the Jaccard index. 1: <span class="html-italic">Chenopodium aristatum</span>, 2: <span class="html-italic">Cleistogenes songorica</span>, 3: <span class="html-italic">Stipa breviflora</span>, 4: <span class="html-italic">Parthenocissus tricuspidate</span>, 5: <span class="html-italic">Convolvulus ammannii</span>, and 6: <span class="html-italic">Salsola collina</span>.</p>
Full article ">Figure 5
<p><math display="inline"><semantics> <mrow> <msup> <mrow> <mi>χ</mi> </mrow> <mrow> <mn>2</mn> </mrow> </msup> </mrow> </semantics></math> test and Jaccard index semi-matrix of population in 25 m × 25 m ungrazed area. The lower diagonal is the <math display="inline"><semantics> <mrow> <msup> <mrow> <mi>χ</mi> </mrow> <mrow> <mn>2</mn> </mrow> </msup> </mrow> </semantics></math> test, and the upper diagonal is the Jaccard index. 1: <span class="html-italic">Stipa breviflora</span>, 2: <span class="html-italic">Cleistogenes songorica</span>, 3: <span class="html-italic">Caragana stenophylla</span>, 4: <span class="html-italic">Convolvulus ammannii</span>, 5: <span class="html-italic">Lagochilus ilicifolium</span>, 6: <span class="html-italic">Chenopodium aristatum</span>, 7: <span class="html-italic">Allium tenuissimum</span>, 8: <span class="html-italic">Ceratoides latens</span>, 9: <span class="html-italic">Kochia prostrata</span>, 10: <span class="html-italic">Salsola collina</span>, 11: <span class="html-italic">Leymus chinensis</span>, 12: <span class="html-italic">Artemisia frigida</span>, 13: <span class="html-italic">Chenopodidm glaucum</span>, 14: <span class="html-italic">Potentilla bifurca</span>, and 15: <span class="html-italic">Stipa krylovii</span>.</p>
Full article ">Figure 6
<p><math display="inline"><semantics> <mrow> <msup> <mrow> <mi>χ</mi> </mrow> <mrow> <mn>2</mn> </mrow> </msup> </mrow> </semantics></math> test and Jaccard index semi-matrix of population in 25 m × 25 m heavily grazed area. The lower diagonal is the <math display="inline"><semantics> <mrow> <msup> <mrow> <mi>χ</mi> </mrow> <mrow> <mn>2</mn> </mrow> </msup> </mrow> </semantics></math> test, and the upper diagonal is the Jaccard index. 1: <span class="html-italic">Stipa breviflora</span>, 2: <span class="html-italic">Chenopodium aristatum</span>, 3: <span class="html-italic">Cleistogenes songorica</span>, 4: <span class="html-italic">Parthenocissus tricuspidate</span>, 5: <span class="html-italic">Convolvulus ammannii</span>, 6: <span class="html-italic">Caragana stenophylla</span>, and 7: <span class="html-italic">Potentilla bifurca</span>.</p>
Full article ">Figure 7
<p><math display="inline"><semantics> <mrow> <msup> <mrow> <mi>χ</mi> </mrow> <mrow> <mn>2</mn> </mrow> </msup> </mrow> </semantics></math> test and Jaccard index semi-matrix of population in 50 m × 50 m ungrazed area. The lower diagonal is the <math display="inline"><semantics> <mrow> <msup> <mrow> <mi>χ</mi> </mrow> <mrow> <mn>2</mn> </mrow> </msup> </mrow> </semantics></math> test, and the upper diagonal is the Jaccard index. 1: <span class="html-italic">Stipa breviflora</span>, 2: <span class="html-italic">Cleistogenes songorica</span>, 3: <span class="html-italic">Caragana stenophylla</span>, 4: <span class="html-italic">Convolvulus ammannii</span>, 5: <span class="html-italic">Lagochilus ilicifolium</span>, 6: <span class="html-italic">Allium tenuissimum</span>, 7: <span class="html-italic">Ceratoides latens</span>, 8: <span class="html-italic">Kochia prostrata</span>, 9: <span class="html-italic">Leymus chinensis</span>, 10: <span class="html-italic">Artemisia frigida</span>, 11: <span class="html-italic">Potentilla bifurca</span>, 12: <span class="html-italic">Chenopodidm glaucum</span>, 13: <span class="html-italic">Chenopodium aristatum</span>, 14: <span class="html-italic">Cleistogenes squarrosa</span>, and 15: <span class="html-italic">Salsola collina</span>.</p>
Full article ">Figure 8
<p><math display="inline"><semantics> <mrow> <msup> <mrow> <mi>χ</mi> </mrow> <mrow> <mn>2</mn> </mrow> </msup> </mrow> </semantics></math> test and Jaccard index semi-matrix of population in 50 m × 50 m heavily grazed area. The lower diagonal is the <math display="inline"><semantics> <mrow> <msup> <mrow> <mi>χ</mi> </mrow> <mrow> <mn>2</mn> </mrow> </msup> </mrow> </semantics></math> test, and the upper diagonal is the Jaccard index. 1: <span class="html-italic">Stipa breviflora</span>, 2: <span class="html-italic">Chenopodium aristatum</span>, 3: <span class="html-italic">Cleistogenes songorica</span>, 4: <span class="html-italic">Parthenocissus tricuspidate</span>, 5: <span class="html-italic">Convolvulus ammannii</span>, 6: <span class="html-italic">Neopallasia pectinate</span>, and 7: <span class="html-italic">Leymus chinensis</span>.</p>
Full article ">Figure 9
<p>Semi-matrix diagram of AC value of desert steppe in the 2.5 m × 2.5 m ungrazed area. 1: <span class="html-italic">Caragana stenophylla</span>, 2: <span class="html-italic">Stipa breviflora</span>, 3: <span class="html-italic">Cleistogenes songorica</span>, 4: <span class="html-italic">Aparagus lucidus lindl</span>, 5: <span class="html-italic">Convolvulus ammannii</span>, 6: <span class="html-italic">Lagochilus ilicifolium</span>, 7: <span class="html-italic">Chenopodium aristatum</span>, 8: <span class="html-italic">Allium tenuissimum</span>, 9: <span class="html-italic">Ceratoides latens</span>, 10: <span class="html-italic">Salsola collina</span>, and 11: <span class="html-italic">Kochia prostrata</span>.</p>
Full article ">Figure 10
<p>Semi-matrix diagram of AC values of desert steppe in 2.5 m × 2.5 m heavily grazed area. 1: <span class="html-italic">Chenopodium aristatum</span>, 2: <span class="html-italic">Cleistogenes songorica</span>, 3: <span class="html-italic">Stipa breviflora</span>, 4: <span class="html-italic">Parthenocissus tricuspidate</span>, 5: <span class="html-italic">Convolvulus ammannii</span>, and 6: <span class="html-italic">Salsola collina</span>.</p>
Full article ">Figure 11
<p>Semi-matrix diagram of AC value of desert steppe in 25 m × 25 m ungrazed area. 1: <span class="html-italic">Stipa breviflora</span>, 2: <span class="html-italic">Cleistogenes songorica</span>, 3: <span class="html-italic">Caragana stenophylla</span>, 4: <span class="html-italic">Convolvulus ammannii</span>, 5: <span class="html-italic">Lagochilus ilicifolium</span>, 6: <span class="html-italic">Chenopodium aristatum</span>, 7: <span class="html-italic">Allium tenuissimum</span>, 8: <span class="html-italic">Ceratoides latens</span>, 9: <span class="html-italic">Kochia prostrata</span>, 10: <span class="html-italic">Salsola collina</span>, 11: <span class="html-italic">Leymus chinensis</span>, 12: <span class="html-italic">Artemisia frigida</span>, 13: <span class="html-italic">Chenopodidm glaucum</span>, 14: <span class="html-italic">Potentilla bifurca</span>, and 15: <span class="html-italic">Stipa krylovii</span>.</p>
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<p>Semi-matrix diagram of AC values of the desert steppe in 25 m × 25 m heavily grazed area. 1: <span class="html-italic">Stipa breviflora</span>, 2: <span class="html-italic">Chenopodium aristatum</span>, 3: <span class="html-italic">Cleistogenes songorica</span>, 4: <span class="html-italic">Parthenocissus tricuspidate</span>, 5: <span class="html-italic">Convolvulus ammannii</span>, 6: <span class="html-italic">Caragana stenophylla</span>, and 7: <span class="html-italic">Potentilla bifurca</span>.</p>
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<p>Semi-matrix diagram of AC value of desert steppe in 50 m × 50 m ungrazed area. 1: <span class="html-italic">Stipa breviflora</span>, 2: <span class="html-italic">Cleistogenes songorica</span>, 3: <span class="html-italic">Caragana stenophylla</span>, 4: <span class="html-italic">Convolvulus ammannii</span>, 5: <span class="html-italic">Lagochilus ilicifolium</span>, 6: <span class="html-italic">Allium tenuissimum</span>, 7: <span class="html-italic">Ceratoides latens</span>, 8: <span class="html-italic">Kochia prostrata</span>, 9: <span class="html-italic">Leymus chinensis</span>, 10: <span class="html-italic">Artemisia frigida</span>, 11: <span class="html-italic">Potentilla bifurca</span>, 12: <span class="html-italic">Chenopodidm glaucum</span>, 13: <span class="html-italic">Chenopodium aristatum</span>, 14: <span class="html-italic">Cleistogenes squarrosa</span>, and 15: <span class="html-italic">Salsola collina</span>.</p>
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<p>Semi-matrix diagram of AC values of desert steppe in 50 m × 50 m heavily grazed area. 1: <span class="html-italic">Stipa breviflora</span>, 2: <span class="html-italic">Chenopodium aristatum</span>, 3: <span class="html-italic">Cleistogenes songorica</span>, 4: <span class="html-italic">Convolvulus ammannii</span>, 5: <span class="html-italic">Parthenocissus tricuspidate</span>, 6: <span class="html-italic">Neopallasia pectinata</span>, and 7: <span class="html-italic">Leymus chinensis</span>.</p>
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<p>Stability fitting curves of M.Godron at different spatial scales in ungrazed area.</p>
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<p>Stability fitting curves of M.Godron at different spatial scales in heavily grazed area.</p>
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22 pages, 942 KiB  
Article
Microbial Indoor Air Quality Within Greenhouses and Polytunnels Is Crucial for Sustainable Horticulture (Malopolska Province, Poland Conditions)
by Jacek Kozdrój, Dariusz Roman Ropek, Krzysztof Frączek, Karol Bulski and Barbara Breza-Boruta
Sustainability 2024, 16(22), 10058; https://doi.org/10.3390/su162210058 - 18 Nov 2024
Abstract
Sustainable horticulture is crucially based on the greenhouse production of vegetables under controlled conditions. In this study, we wanted to learn how cultivated plants may impact indoor air quality and whether the workers can be exposed to bioaerosols in a similar way in [...] Read more.
Sustainable horticulture is crucially based on the greenhouse production of vegetables under controlled conditions. In this study, we wanted to learn how cultivated plants may impact indoor air quality and whether the workers can be exposed to bioaerosols in a similar way in these settings. The study objective was to test the hypothesis that the microbial concentrations, distribution of bioaerosol particle sizes, and composition of the airborne microbiome are specific to greenhouses, polytunnels, and open-air sites. The air samples were collected to assess the concentration of total culturable bacteria (TCB), fungi, actinomycetes, and β-haemolytic bacteria and for the identification of bacterial and fungal strains. Higher concentrations of TCB and fungi were found in the greenhouse (log 3.71 and 3.49 cfu m−3, respectively) than in polytunnels (log 2.60–2.48 and 2.51–2.31 cfu m−3, respectively) during the vegetation of cucumbers. These airborne microbes were represented by a significant contribution of the respirable fraction with a distinct contribution of fine particles in size below 4.7 µm. Cultivation of cucumbers resulted in the higher emission of airborne microorganisms in contrast with growing herbs such as oregano and basil. In total, 35 different bacteria and 12 fungal species, including pathogenic or allergenic agents, were identified within the studied sites. The workers can be exposed to increased concentrations of TCB and fungi in the greenhouse during the plant vegetation. It might be recommended to properly manage greenhouses and polytunnels, dispose of dust sources, and maintain appropriate ventilation to sustain relevant air quality. Full article
(This article belongs to the Special Issue Soil, Plant and Human Health in Sustainable Environment)
24 pages, 1473 KiB  
Protocol
Switching Mediterranean Consumers to Mediterranean Sustainable Healthy Dietary Patterns (SWITCHtoHEALTHY): Study Protocol of a Multicentric and Multi-Cultural Family-Based Nutritional Intervention Study
by Lorena Calderón-Pérez, Alícia Domingo, Josep M. del Bas, Biotza Gutiérrez, Anna Crescenti, Djamel Rahmani, Amèlia Sarroca, José Maria Gil, Kenza Goumeida, Tianyu Zhang Jin, Metin Güldaş, Çağla Erdoğan Demir, Asmaa El Hamdouchi, Lazaros P. Gymnopoulos, Kosmas Dimitropoulos, Perla Degli Innocenti, Alice Rosi, Francesca Scazzina, Eva Petri, Leyre Urtasun, Giuseppe Salvio, Marco de la Feld and Noemi Boquéadd Show full author list remove Hide full author list
Nutrients 2024, 16(22), 3938; https://doi.org/10.3390/nu16223938 (registering DOI) - 18 Nov 2024
Abstract
Background/Objectives: Populations in Mediterranean countries are abandoning the traditional Mediterranean diet (MD) and lifestyle, shifting towards unhealthier habits due to profound cultural and socioeconomic changes. The SWITCHtoHEALTHY project aims to demonstrate the effectiveness of a multi-component nutritional intervention to improve the adherence of [...] Read more.
Background/Objectives: Populations in Mediterranean countries are abandoning the traditional Mediterranean diet (MD) and lifestyle, shifting towards unhealthier habits due to profound cultural and socioeconomic changes. The SWITCHtoHEALTHY project aims to demonstrate the effectiveness of a multi-component nutritional intervention to improve the adherence of families to the MD in three Mediterranean countries, thus prompting a dietary behavior change. Methods: A parallel, randomized, single-blinded, and controlled multicentric nutritional intervention study will be conducted over 3 months in 480 families with children and adolescents aged 3–17 years from Spain, Morocco, and Turkey. The multi-component intervention will combine digital interactive tools, hands-on educational materials, and easy-to-eat healthy snacks developed for this study. Through the developed SWITCHtoHEALTHY app, families will receive personalized weekly meal plans, which also consider what children eat at school. The engagement of all family members will be prompted by using a life simulation game. In addition, a set of activities and educational materials for adolescents based on a learning-through-playing approach will be codesigned. Innovative and sustainable plant-based snacks will be developed and introduced into the children’s dietary plan as healthy alternatives for between meals. By using a full-factorial design, families will be randomized into eight groups (one control and seven interventions) to test the independent and combined effects of each component (application and/or educational materials and/or snacks). The impact of the intervention on diet quality, economy, and the environment, as well as on classical anthropometric parameters and vital signs, will be assessed in three different visits. The COM-B behavioral model will be used to assess essential factors driving the behavior change. The main outcome will be adherence to the MD assessed through MEDAS in adults and KIDMED in children and adolescents. Conclusions: SWITCHtoHEALTHY will provide new insights into the use of sustained models for inducing dietary and lifestyle behavior changes in the family setting. It will facilitate generating, boosting, and maintaining the switch to a healthier MD dietary pattern across the Mediterranean area. Registered Trial, National Institutes of Health, ClinicalTrials.gov (NCT06057324). Full article
(This article belongs to the Special Issue Advances in Sustainable Healthy Diets)
37 pages, 2386 KiB  
Review
Cajaninstilbene Acid and Its Derivative as Multi-Therapeutic Agents: A Comprehensive Review
by Wen Hou, Lejun Huang, Jinyang Wang, Walter Luyten, Jia Lai, Zhinuo Zhou, Sishuang Kang, Ping Dai, Yanzhu Wang, Hao Huang and Jinxia Lan
Molecules 2024, 29(22), 5440; https://doi.org/10.3390/molecules29225440 (registering DOI) - 18 Nov 2024
Abstract
: Pigeon pea (Cajanus cajan (L.) Millsp.) is a traditional Chinese medicinal plant widely utilized in folk medicine due to its significant pharmacological and nutritional properties. Cajaninstilbene acid (CSA), a stilbene compound derived from pigeon pea leaves, has been extensively investigated [...] Read more.
: Pigeon pea (Cajanus cajan (L.) Millsp.) is a traditional Chinese medicinal plant widely utilized in folk medicine due to its significant pharmacological and nutritional properties. Cajaninstilbene acid (CSA), a stilbene compound derived from pigeon pea leaves, has been extensively investigated since the 1980s. A thorough understanding of CSA’s mechanisms of action and its therapeutic effects on various diseases is crucial for developing novel therapeutic approaches. This paper presents an overview of recent research advancements concerning the biological activities and mechanisms of CSA and its derivatives up to February 2024. The review encompasses discussions on the in vivo metabolism of CSA and its derivatives, including antipathogenic micro-organisms activity, anti-tumor activity, systematic and organ protection activity (such as bone protection, cardiovascular protection, neuroprotection), anti-inflammatory activity, antioxidant activity, immune regulation as well as action mechanism of CSA and its derivatives. The most studied activities are antipathogenic micro-organisms activities. Additionally, the structure–activity relationships of CSA and its derivatives as well as the total synthesis of CSA are explored, highlighting the potential for developing new pharmaceutical agents. This review aims to provide a foundation for future clinical applications of CSA and its derivatives. Full article
(This article belongs to the Special Issue Advances in Natural Products and Their Biological Activities)
22 pages, 3933 KiB  
Article
A Quasi Time-Domain Method for Fatigue Analysis of Reactor Pressure Vessels in Floating Nuclear Power Plants in Marine Environments
by Fuxuan Ma, Huanming Li, Meng Zhang and Xiangiang Qu
J. Mar. Sci. Eng. 2024, 12(11), 2085; https://doi.org/10.3390/jmse12112085 - 18 Nov 2024
Abstract
The reactor pressure vessel (RPV) in onshore nuclear power plants is typically analysed for fatigue life by considering the temperature, internal pressure, and seismic effects using a simplified time-domain fatigue analysis. In contrast, the frequency-domain fatigue analysis method is commonly employed to assess [...] Read more.
The reactor pressure vessel (RPV) in onshore nuclear power plants is typically analysed for fatigue life by considering the temperature, internal pressure, and seismic effects using a simplified time-domain fatigue analysis. In contrast, the frequency-domain fatigue analysis method is commonly employed to assess the fatigue life of ship structures. The RPV of a floating nuclear power plant (FNPP) is subjected to a combination of temperature, internal pressure, and wave loads in the marine environment. Consequently, it is essential to effectively integrate the frequency-domain fatigue analysis method used for hull structures with the time-domain fatigue analysis method for RPVs in FNPPs or, alternatively, to develop a suitable method that effectively accounts for the temperature, internal pressure, and wave loads. In this study, a quasi-time-domain method is proposed for the fatigue analysis of RPVs in FNPPs. In this method, secondary components of marine environmental loads are filtered out using principal component analysis. Subsequently, the stress spectrum induced by waves is transformed into a stress time history. Fatigue stress under the combined influence of temperature, internal pressure, and wave loads is then obtained through a stress component superposition method. Finally, the accuracy of the quasi-time-domain method was validated through three numerical examples. The results indicate that the calculated values obtained by the quasi-time-domain method are slightly higher than those obtained by the traditional time-domain method, with a maximum deviation of no more than 24%. Additionally, the computation time of the quasi-time-domain method is reduced by 98.67% compared to the traditional time-domain method. Full article
22 pages, 5047 KiB  
Article
Unlocking the Secrets of Corn: Physiological Responses and Rapid Forecasting in Varied Drought Stress Environments
by Wenlong Song, Kaizheng Xiang, Yizhu Lu, Mengyi Li, Hongjie Liu, Long Chen, Xiuhua Chen and Haider Abbas
Remote Sens. 2024, 16(22), 4302; https://doi.org/10.3390/rs16224302 (registering DOI) - 18 Nov 2024
Abstract
Understanding the intricate relationship between drought stress and corn yield is crucial for ensuring food security and sustainable agriculture in the face of climate change. This study investigates the subtle effects of drought stress on corn physiological, morphological, and spectral characteristics at different [...] Read more.
Understanding the intricate relationship between drought stress and corn yield is crucial for ensuring food security and sustainable agriculture in the face of climate change. This study investigates the subtle effects of drought stress on corn physiological, morphological, and spectral characteristics at different growth stages, in order to construct a new drought index to characterize drought characteristics, so as to provide valuable insights for maize recovery mechanism and yield prediction. Specific conclusions are as follows. Firstly, the impact of drought stress on corn growth and development shows a gradient effect, with the most significant effects observed during the elongation stage and tasseling stage. Notably, Soil and Plant Analyzer Development (SPAD) and Leaf Area Index (LAI) are significantly affected during the silking stage, while plant height and stem width remain relatively unaffected. Secondly, spectral feature analysis reveals that, from the elongation stage to the silking stage, canopy reflectance exhibits peak–valley variations. Drought severity correlates positively with reflectance in the visible and shortwave infrared bands and negatively with reflectance in the near-infrared band. Canopy spectra during the silking stage are more affected by moderate and severe drought stress. Thirdly, LAI shows a significant positive correlation with yield, indicating its reliability in explaining yield variations. Finally, the yield-related drought index (YI) constructed based on Convolutional Neural Network (CNN), Random Forest (RF) and Multiple Linear Regression (MLR) methods has a good effect on revealing drought characteristics (R = 0.9332, p < 0.001). This study underscores the importance of understanding corn responses to drought stress at various growth stages for effective yield prediction and agricultural management strategies. Full article
15 pages, 2646 KiB  
Article
A Novel Gene, OsRLCK191, Involved in Culm Strength Improving Lodging Resistance in Rice
by Huilin Chang, Hanjing Sha, Shiwei Gao, Qing Liu, Yuqiang Liu, Cheng Ma, Bowen Shi and Shoujun Nie
Int. J. Mol. Sci. 2024, 25(22), 12382; https://doi.org/10.3390/ijms252212382 - 18 Nov 2024
Abstract
Lodging is one of the major problems in rice production. However, few genes that can explain the culm strength within the temperate japonica subspecies have been identified. In this study, we identified OsRLCK191, which encodes receptor-like cytoplasmic kinase and plays critical roles [...] Read more.
Lodging is one of the major problems in rice production. However, few genes that can explain the culm strength within the temperate japonica subspecies have been identified. In this study, we identified OsRLCK191, which encodes receptor-like cytoplasmic kinase and plays critical roles in culm strength. OsRLCK191 mutants were produced by the CRISPR-Cas9 DNA-editing system. Compared with wild types (WTs), the bending moment of the whole plant (WP), the bending moment at breaking (BM), and the section modulus (SM) were decreased in rlck191 significantly. Although there is no significant decrease in the culm length of rlck191 compared with the WT; in the mutant, except the length of the fourth internode being significantly increased, the lengths of other internodes are significantly shortened. In addition, the yield traits of panicle length, thousand-seed weight, and seed setting rate decreased significantly in rlck191. Moreover, RNA-seq experiments were performed at an early stage of rice panicle differentiation in shoot apex. The differentially expressed genes (DEGs) are mainly involved in cell wall biogenesis, cell wall polysaccharide metabolic processes, cellar component biogenesis, and DNA-binding transcription factors. Transcriptome analysis of the cell wall biological process pathways showed that major genes that participated in the cytokinin oxidase/dehydrogenase family, cellulose synthase catalytic subunit genes, and ethylene response factor family transcription factor were related to culm strength. Our research provides an important theoretical basis for analyzing the lodging resistance mechanism and lodging resistance breeding of temperate japonica. Full article
(This article belongs to the Section Molecular Plant Sciences)
18 pages, 3388 KiB  
Article
In Vitro Evaluation of Iraqi Kurdistan Tomato Accessions Under Drought Stress Conditions Using Polyethylene Glycol-6000
by Nawroz Abdul-razzak Tahir, Kamaran Salh Rasul, Djshwar Dhahir Lateef, Rebwar Rafat Aziz and Jalal Omer Ahmed
Life 2024, 14(11), 1502; https://doi.org/10.3390/life14111502 - 18 Nov 2024
Abstract
Drought is one of the major abiotic stresses that affect plant growth and productivity, and plant stress responses are affected by both the intensity of stress and genotype. In Iraqi Kurdistan, tomato plants play a significant role in the country’s economy. Due to [...] Read more.
Drought is one of the major abiotic stresses that affect plant growth and productivity, and plant stress responses are affected by both the intensity of stress and genotype. In Iraqi Kurdistan, tomato plants play a significant role in the country’s economy. Due to climate change, which causes soil moisture to diminish, the crop’s growth and yield have been dropping in recent years. Accordingly, the effects of simulated drought stress on germination parameters were assessed in 64 tomato accessions gathered from the Iraqi Kurdistan region in order to identify sensitive and tolerant accessions. In this respect, the responses associated with drought stress were observed phenotypically and biochemically. Germination percentage (GP) and morphological characteristics such as root length (RL), shoot length (SL), and shoot fresh weight (SFW) were significantly reduced in both stress treatments with polyethylene glycol (PEG-6000) (7.5% PEG and 15% PEG). On the other hand, significant changes in biochemical profiles such as proline content (PC), soluble sugar content (SSC), total phenolic content (TPC), antioxidant activity (AC), guaiacol peroxidase (GPA), catalase (CAT), and lipid peroxidation (LP) in tomato accessions were detected; all biochemical traits were increased in most tomato accessions under the PEG-induced treatments compared to the control treatment (0.0% PEG). Three tomato accessions (AC61 (Raza Pashayi), AC9 (Wrdi Be Tow), and AC63 (Sandra)) were found to be the most tolerant accessions under all drought conditions, whereas the performances of the other tested accessions (AC13 (Braw), AC30 (Yadgar), and AC8 (Israili)) were inferior. The OMIC analysis identified the biomarker parameters for differentiating the highly, moderately, and low tolerant groups as PC, SSC, and TPC. This study shows that early PEG-6000 screening for drought stress may help in choosing a genotype that is suitable for growth in water-stressed environments. Hence, Raza Pashayi, Wrdi Be Tow, and Sandra accessions, which had great performances under drought conditions, can be candidates for selection in a breeding program to improve the growth of plants and production in the areas that face water limits. Full article
(This article belongs to the Special Issue Plant Biotic and Abiotic Stresses 2024)
26 pages, 1847 KiB  
Article
Long-Term Cumulative Effect of Management Decisions on Forest Structure and Biodiversity in Hemiboreal Forests
by Teele Paluots, Jaan Liira, Mare Leis, Diana Laarmann, Eneli Põldveer, Jerry F. Franklin and Henn Korjus
Forests 2024, 15(11), 2035; https://doi.org/10.3390/f15112035 - 18 Nov 2024
Abstract
We evaluated the long-term impacts of various forest management practices on the structure and biodiversity of Estonian hemiboreal forests, a unique ecological transition zone between temperate and boreal forests, found primarily in regions with cold winters and moderately warm summers, such as the [...] Read more.
We evaluated the long-term impacts of various forest management practices on the structure and biodiversity of Estonian hemiboreal forests, a unique ecological transition zone between temperate and boreal forests, found primarily in regions with cold winters and moderately warm summers, such as the northern parts of Europe, Asia, and North America. The study examined 150 plots across stands of different ages (65–177 years), including commercial forests and Natura 2000 habitat 9010* “Western Taiga”. These plots varied in stand origin—multi-aged (trees of varying ages) versus even-aged (uniform tree ages), management history—historical (practices before the 1990s) and recent (post-1990s practices), and conservation status—protected forests (e.g., Natura 2000 areas) and commercial forests focused on timber production. Data on forest structure, including canopy tree diameters, deadwood volumes, and species richness, were collected alongside detailed field surveys of vascular plants and bryophytes. Management histories were assessed using historical maps and records. Statistical analyses, including General Linear Mixed Models (GLMMs), Multi-Response Permutation Procedures (MRPP), and Indicator Species Analysis (ISA), were used to evaluate the effects of origin, management history, and conservation status on forest structure and species composition. Results indicated that multi-aged origin forests had significantly higher canopy tree diameters and deadwood volumes compared to even-aged origin stands, highlighting the benefits of varied-age management for structural diversity. Historically managed forests showed increased tree species richness, but lower deadwood volumes, suggesting a biodiversity–structure trade-off. Recent management, however, negatively impacted both deadwood volume and understory diversity, reflecting short-term forestry consequences. Protected areas exhibited higher deadwood volumes and bryophyte richness compared to commercial forests, indicating a small yet persistent effect of conservation strategies in sustaining forest complexity and biodiversity. Indicator species analysis identified specific vascular plants and bryophytes as markers of long-term management impacts. These findings highlight the ecological significance of integrating historical legacies and conservation priorities into modern management to support forest resilience and biodiversity. Full article
17 pages, 9077 KiB  
Article
Diversity and Virulence of Diaporthe Species Associated with Peach Trunk Diseases in China
by Ying Zhou, Wei Zhang, Pranami D. Abeywickrama, Zhizheng He, Zhixiang Zhang, Yonghua Li, Shifang Li, Zaifeng Fan and Jiye Yan
Plants 2024, 13(22), 3238; https://doi.org/10.3390/plants13223238 - 18 Nov 2024
Abstract
Peach (Prunus persica L.) is one of the most important and oldest stone fruits grown in China. Though Diaporthe species have more commonly been reported as plant pathogens, endophytes and saprophytes with a wide range of plant hosts, little is known about [...] Read more.
Peach (Prunus persica L.) is one of the most important and oldest stone fruits grown in China. Though Diaporthe species have more commonly been reported as plant pathogens, endophytes and saprophytes with a wide range of plant hosts, little is known about the Diaporthe species associated with peach trunk diseases in China. In the present study, forty-four Diaporthe isolates were obtained from trees with peach branch canker, shoot blight and gummosis symptoms in four provinces in China. Based on a combination of morphology and multi-locus sequence analysis of the rDNA internal transcribed spacer region (ITS), calmodulin (cal), translation elongation factor 1-α (tef1) and β-tubulin (tub2), these Diaporthe isolates were assigned to four species. Detailed descriptions and illustrations of all of the species, D. arecae, D. caulivora, D. discoidispora and D. eres, are provided. This study further reports the first host association of D. caulivora and D. discoidispora on peaches worldwide. The pathogenicity experiment results revealed that D. arecae was the most aggressive species, whereas D. discoidispora was the least aggressive on detached peach shoots. This study provides new insights into the fungi associated with peach trunk diseases in China, and the results of this study may help to facilitate routine diagnosis and planning of suitable plant disease management strategies. Full article
(This article belongs to the Special Issue Mycology and Plant Pathology)
Show Figures

Figure 1

Figure 1
<p>Phylogenetic tree generated from maximum likelihood analysis based on combined ITS, <span class="html-italic">cal</span>, <span class="html-italic">tef1</span> and <span class="html-italic">tub2</span> sequence data for the <span class="html-italic">Diaporthe arecae</span> species complex. Bootstrap support values for maximum likelihood (ML-BS ≥ 70%) and Bayesian posterior probabilities (PP ≥ 0.90) are shown at the nodes. Type strains are indicated in bold. The scale bar represents the expected number of changes per site. The tree is rooted with <span class="html-italic">Diaporthella coryli</span> (CBS 121124). Isolates obtained from this study are marked in red.</p>
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<p>Phylogenetic tree generated from maximum likelihood analysis based on combined ITS, <span class="html-italic">cal</span>, <span class="html-italic">tef1</span> and <span class="html-italic">tub2</span> sequence data for the <span class="html-italic">Diaporthe eres</span> and related species. Bootstrap support values for maximum likelihood (ML-BS ≥ 70%) and Bayesian posterior probabilities (PP ≥ 0.90) are shown at the nodes. Type strains are indicated in bold. The scale bar represents the expected number of changes per site. The tree is rooted with <span class="html-italic">Diaporthe virgiliae</span> (CMW40748). Isolates obtained from this study are marked in red.</p>
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<p>Phylogenetic tree generated from maximum likelihood analysis based on combined ITS, <span class="html-italic">cal</span>, <span class="html-italic">tef1</span> and <span class="html-italic">tub2</span> sequence data for the <span class="html-italic">Diaporthe eres</span> and related species. Bootstrap support values for maximum likelihood (ML-BS ≥ 70%) and Bayesian posterior probabilities (PP ≥ 0.90) are shown at the nodes. Type strains are indicated in bold. The scale bar represents the expected number of changes per site. The tree is rooted with <span class="html-italic">Diaporthe virgiliae</span> (CMW40748). Isolates obtained from this study are marked in red.</p>
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<p>Phylogenetic tree generated from maximum likelihood analysis based on combined ITS, <span class="html-italic">cal</span>, <span class="html-italic">tef1</span> and <span class="html-italic">tub2</span> sequence data for the <span class="html-italic">Diaporthe</span> species. Bootstrap support values for maximum likelihood (ML-BS ≥ 70%) and Bayesian posterior probabilities (PP ≥ 0.90) are shown at the nodes. Type strains are indicated in bold. The scale bar represents the expected number of changes per site. The tree is rooted with <span class="html-italic">Diaporthe eres</span> (CBS 587.79). Isolates obtained from this study are marked in red.</p>
Full article ">Figure 3 Cont.
<p>Phylogenetic tree generated from maximum likelihood analysis based on combined ITS, <span class="html-italic">cal</span>, <span class="html-italic">tef1</span> and <span class="html-italic">tub2</span> sequence data for the <span class="html-italic">Diaporthe</span> species. Bootstrap support values for maximum likelihood (ML-BS ≥ 70%) and Bayesian posterior probabilities (PP ≥ 0.90) are shown at the nodes. Type strains are indicated in bold. The scale bar represents the expected number of changes per site. The tree is rooted with <span class="html-italic">Diaporthe eres</span> (CBS 587.79). Isolates obtained from this study are marked in red.</p>
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<p><span class="html-italic">Diaporthe arecae</span> (JZB320302). (<b>A</b>,<b>B</b>) Colony on PDA (front and reverse); (<b>C</b>) conidiomata on PDA; (<b>D</b>) conidiophores; (<b>E</b>) alpha conidia; (<b>F</b>) beta conidia. Scale bars: 200 μm (<b>C</b>); 10 μm (<b>D</b>–<b>F</b>).</p>
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<p><span class="html-italic">Diaporthe eres</span> (JZB320287) (<b>A</b>,<b>B</b>) Colony on PDA (front and reverse) (<b>C</b>) Culture on PDA and conidiomata (<b>D</b>) conidiomata on PDA (<b>E</b>) Conidiophores (<b>F</b>) Alpha and Beta conidia (<b>G</b>) Beta conidia. Scale bars: 200 μm (<b>D</b>); 10 μm (<b>E</b>–<b>G</b>).</p>
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<p><span class="html-italic">Diaporthe caulivora</span> (JZB320306). (<b>A</b>,<b>B</b>) Colony on PDA (front and reverse). (<b>C</b>) Culture on PDA and conidiomata. (<b>D</b>) Conidiomata on PDA. (<b>E</b>) Conidiophores. (<b>F</b>) Alpha conidia. (<b>G</b>) Gamma conidia. Scale bars: 200 μm (<b>D</b>); 5 μm (<b>E</b>–<b>G</b>).</p>
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<p><span class="html-italic">Diaporthe discoidispora</span> (JZB320298) (<b>A</b>,<b>B</b>) Colony on PDA (front and reverse). (<b>C</b>) Culture on PDA and conidiomata. (<b>D</b>) Conidiomata on PDA. (<b>E</b>) Conidiophores. (<b>F</b>) Alpha conidia. (<b>G</b>) Beta conidia. Scale bars: 200 μm (<b>D</b>); 10 μm (<b>E</b>–<b>G</b>).</p>
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<p>Disease lesions developed on peach green shoots after 7 days of inoculation of <span class="html-italic">Diaporthe</span> isolates. (<b>A</b>–<b>J</b>) <span class="html-italic">D. eres</span>; (<b>A</b>): JZB320279; (<b>B</b>): JZB320280; (<b>C</b>): JZB320281; (<b>D</b>): JZB320282; (<b>E</b>): JZB320283; (<b>F</b>): JZB320287; (<b>G</b>): JZB320288; (<b>H</b>): JZB320290; (<b>I</b>): JZB320293; (<b>J</b>): JZB320295. (<b>K</b>,<b>L</b>) <span class="html-italic">D. discoidispora</span>; (<b>K</b>): JZB320298; (<b>L</b>): JZB320299. (<b>M</b>,<b>N</b>) <span class="html-italic">D. arecae</span>; (<b>M</b>): JZB320302; (<b>N</b>): JZB320303. (<b>O</b>,<b>P</b>) <span class="html-italic">D. caulivora</span>; (<b>O</b>): JZB320305; (<b>P</b>): JZB320306. (<b>Q</b>,<b>R</b>): control.</p>
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<p>Average lesion length (cm) resulting from inoculation trials with <span class="html-italic">Prunus persica</span> L. on the seventh day. Vertical bars represent the standard error of means. Different letters above the bars indicate treatments that were significantly different (<span class="html-italic">p</span> = 0.05). <span class="html-italic">Diaporthe eres</span>: JZB320279, JZB320280, JZB320281, JZB320282, JZB320283, JZB320287, JZB320288, JZB320290, JZB320293, JZB320295; <span class="html-italic">D</span>. <span class="html-italic">discoidispora</span>: JZB320298, JZB320299, <span class="html-italic">D. arecae</span>: JZB320302, JZB320303. <span class="html-italic">D</span>. <span class="html-italic">caulivora</span>: JZB320305, JZB320306.</p>
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27 pages, 1154 KiB  
Review
A Critical Review of Recent Advances in Maize Stress Molecular Biology
by Lingbo Meng, Jian Zhang and Nicholas Clarke
Int. J. Mol. Sci. 2024, 25(22), 12383; https://doi.org/10.3390/ijms252212383 - 18 Nov 2024
Abstract
With the intensification of global climate change and environmental stress, research on abiotic and biotic stress resistance in maize is particularly important. High temperatures and drought, low temperatures, heavy metals, salinization, and diseases are widespread stress factors that can reduce maize yields and [...] Read more.
With the intensification of global climate change and environmental stress, research on abiotic and biotic stress resistance in maize is particularly important. High temperatures and drought, low temperatures, heavy metals, salinization, and diseases are widespread stress factors that can reduce maize yields and are a focus of maize-breeding research. Molecular biology provides new opportunities for the study of maize and other plants. This article reviews the physiological and biochemical responses of maize to high temperatures and drought, low temperatures, heavy metals, salinization, and diseases, as well as the molecular mechanisms associated with them. Special attention is given to key transcription factors in signal transduction pathways and their roles in regulating maize stress adaptability. In addition, the application of transcriptomics, genome-wide association studies (GWAS), and QTL technology provides new strategies for the identification of molecular markers and genes for maize-stress-resistance traits. Crop genetic improvements through gene editing technologies such as the CRISPR/Cas system provide a new avenue for the development of new stress-resistant varieties. These studies not only help to understand the molecular basis of maize stress responses but also provide important scientific evidence for improving crop tolerance through molecular biological methods. Full article
(This article belongs to the Special Issue Recent Advances in Maize Stress Biology)
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