Search Results (1,762)

The symptoms of foodborne illness caused by Bacillus cereus often go unreported, complicating the effectiveness of conventional chemical and physical methods used to inhibit its growth in food production. This challenge, combined with the increasing use of lactic acid bacteria (LAB) in the food industry and consumer preference for minimally processed products, prompted this study. The antibacterial activity of diffusible substances produced by Lactococcus lactis ATCC 11454 against Bacillus cereus NC11143 and Escherichia coli K-12 MG1655 was investigated using a non-contact co-culture model utilising deMann Rogosa and Sharpe broth, with glucose as a carbon source. This study employed plate counting and flow cytometry to assess the impact of these substances on bacterial growth and to analyse their composition and antimicrobial efficacy. The co-culture of Lactococcus lactis ATCC 11454 resulted in the production of a stable antimicrobial peptide, which was heat resistant and acid tolerant. Purification was achieved via ammonium sulphate precipitation and preparative HPLC, yielding a peptide with a molecular mass of 3.3 kDa, with daughter ion fractions similar to nisin A. Antimicrobial activity studies demonstrated that the diffusible substances effectively inhibited B. cereus growth over a period of eight days and exhibited bactericidal activity, killing 99% of the B. cereus cells. Additionally, these substances also inhibited Escherichia coli K-12 MG1655 grown under similar conditions. Comparative analysis revealed that in the co-culture assay, L. lactis produced a 50% higher yield of the antimicrobial peptides compared to pure cultures. Similarly, the specific growth rate of L. lactis was four times higher. With respect to protein purification and concentration, ammonium sulphate precipitation coupled with solid phase extraction was most effective in the purification and concentration of the diffusible substances. The findings provide a basis for utilising bacteriocin-producing strains as a preservation method, offering an alternative to traditional chemical and physical control approaches especially for the food industry. Full article

The causes of the size effect (SE) and loading rate effect (LR) for rocks remain unclear. Based on this, a gypsum-mixed material was used to simulate sandstone, where the dosing ratio was 7.5% river sand, 17.5% quartz, 58.3% $\alpha$-high-strength gypsum, and 16.7% water. The specimens were designed to have a height-to-diameter ratio (HDR) of 0.6~2, and three strain rates (SRs)—static, quasi-dynamic, and dynamic—were used to perform single-factor rotational uniaxial compression experiments. PFC^2D was used to numerically simulate the damage pattern of a sandstone-like specimen. The results showed that the physical parameters did not change monotonically, as was previously found. The main reason for this is that the end-face friction effect (EFE) is generated when the dynamic SR or the HDR is 0.6~1, with a damage pattern of “X”. Under mechanical analysis, the power consumed by the EFE was inversely proportional to the HDR and directly proportional to the LR, and it can reduce the actual amount of energy transferred inside the specimen. This paper may provide a foundation for the study of non-linear hazards in coal and rock. Full article

Indium phosphide (InP) is widely utilized in the fields of electronics and photovoltaics due to its high electron mobility and high photoelectric conversion efficiency. Strain engineering has been extensively employed in semiconductor devices to adjust physical properties and enhance material performance. In the present work, the band structure and electronic effective mass of InP under different strains are investigated by ab initio calculations. The results show that InP consistently exhibits a direct bandgap under different strains. Both uniaxial strain and biaxial tensile strain exhibit linear effects on the change in bandgap values. However, the bandgap of InP is significantly influenced by uniaxial compressive strain and biaxial tensile strain, respectively. The study of the InP bandgap under different hydrostatic pressures reveals that InP becomes metallic when the pressure is less than −7 GPa. Furthermore, strain also leads to changes in effective mass and the anisotropy of electron mobility. The studies of electronic properties under different strain types are of great significance for broadening the application of InP devices. Full article

Regulating the nucleation temperature and growth rates during inverse temperature crystallization (ITC) is vital for obtaining high-quality perovskite single crystals via this technique. Precise control over these parameters enables growing crystals optimized for various optoelectronic devices. In this study, it is demonstrated that incorporating a 1-butyl-3-methylimidazolium bromide (BMIB) ionic liquid into the precursor solution of cesium lead bromide (CsPbBr₃) brings about a dual enhancement effect. This includes a reduction in nucleation temperature from 85 °C to 65 °C and a significant improvement in both optoelectronic characteristics and crystal properties. The CsPbBr₃ single crystals grown using ITC with BMIB added (method (2)) demonstrate improved chemical and physical properties (crystallinity, lattice strain, nonradioactive recombination, and trap density) compared to CsPbBr₃ single crystals produced through conventional 85 °C ITC alone (method (1)). The exceptional quality of CsPbBr₃ single crystals produced with the inclusion of BMIB allowed for the development of a highly responsive optoelectronic device, demonstrating heightened sensitivity to green light. The findings of this investigation reveal that the growth of perovskite single crystals assisted by ionic liquid exerts a substantial impact on the characteristics of the crystals. This influence proves advantageous for the development of optoelectronic devices based on single crystals. Full article

Strong mine pressure often emerges when the working face of the lower coal seam in a closely spaced coal seam system passes through the remaining coal pillar in the overlying goaf. This study investigates the law of overburden movement and the manifestation of mine pressure during mining under the remaining coal pillar. A physical model measuring 2.5 × 0.2 × 1.503 m is constructed. Fiber Bragg grating sensing technology (FBG) and Brillouin optical time domain analysis technology (BOTDA) are employed in the physical model experiment to monitor the internal strain of the overlying rock as the working face advances. This study determines the laws of overlying rock fracture and working face pressure while mining coal seams beneath the remaining coal pillar. It analyzes the relationship between the pressure at the working face and the strain characteristics of the horizontally distributed optical fiber. A fiber grating characterization method is established for the stress evolution law of overlying rock while passing the remaining coal pillar. The experimental results indicated that the fracture angle of overlying rock gradually decreases during the mining stage through and after the coal pillar. In the mining stage through the coal pillar, the cycle pressure step distance of the working face is reduced by 33.3% compared to the stage after mining through the coal pillar. Initially, the strain pattern of the horizontal optical fiber is unimodal when pressure is first applied to the working face, and it transitions from unimodal to bimodal during periodic pressure. The peak value of fiber Bragg grating compressive strain and the range of influence of advanced support pressure are 3.6 times and 4.8 times, respectively, before passing through the remaining coal pillar. Finally, the accuracy of the FBG characterization method is verified by comparing it to the monitoring curve of the coal seam floor pressure sensor. The research results contribute to applying fiber optic sensing technology in mining physical model experiments. Full article

Polyhydroxyalkanoate (PHA), specifically poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) (P(3HB-co-3HHx), PHBHHx) with physical properties governed by the 3-hydroxyhexanoate (3HHx) mole fraction, is a promising bioplastic. Although engineered strains used to produce P(3HB-co-3HHx) with various 3HHx mole contents and fermentation techniques have been studied, mass production with specific 3HHx fractions and monomers depends on the batch, supply of substrates, and strains, resulting in the time-consuming development of strains and complex culture conditions for P(3HB-co-3HHx). To overcome these limitations, we blended poly(3-hydroxybutyrate) [(P(3HB), produced from C. necator H16] and P(3HB-co-20 mol%3HHx) [from C. necator 2668/pCB81] to prepare films with various 3HHx contents. We evaluated the molecular weight and physical, thermal, and mechanical properties of these films and confirmed the influence of the 3HHx monomer content on the mechanical and thermal properties as well as degradability of the blended P(3HB-co-3HHx) films containing various 3HHx mole fractions, similar to that of original microbial-based P(3HB-co-3HHx). Moreover, the degradation rate analyzed via Microbulbifer sp. was >76% at all blending ratios within 2 days, whereas a weaker effect of the 3HHx mole fraction of the blended polymer on degradation was observed. P(3HB-co-3HHx) could be produced via simple blending using abundantly produced P(3HB) and P(3HB-co-20mol%HHx), and the resulting copolymer is applicable as a biodegradable plastic. Full article

Agricultural practices affect the physical, chemical, and biological properties of soil in agroecosystems. This study evaluated the impact of food production strategies on the rhizosphere microbial communities in the Yaqui Valley, Mexico, on key crops (Medicago sativa, Brassica oleracea, Asparagus officinalis, Phaseolus vulgaris, Citrus sinensis, Zea mays, Solanum tuberosum, Triticum durum, and an undisturbed native ecosystem). Soil samples were collected from 30 cm depths across one-hectare fields and analyzed for bulk density, pH, organic matter content, and electrical conductivity. Standardized methods were used for these analyses, along with microbial isolation through culturing, PCR amplification, and DNA sequencing for microbial identification. The use of synthetic fertilizers in the region was linked to increased salinity and soil compaction. Organic matter content was notably low at ≤1.4%, which negatively impacted microbial diversity. A total of 317 microbial strains were isolated, with bacteria comprising 73% and fungi 27%. Bacillus was the most dominant bacterial genus (41% of isolates), while Aspergillus was the most abundant fungal genus (31% of isolates). Crop-specific microbial strains were identified. This study provides the first detailed insight into how agricultural practices shape microbial communities in the Yaqui Valley’s major crops, highlighting the link between soil properties and microbial diversity. Full article

Actinobacteria, especially the genus Streptomyces, have been shown to be potential biocontrol agents for phytopathogenic bacteria. Bacteria spot disease caused by Xanthomonas spp. may severely affect chili pepper (Capsicum annuum) crops with a subsequent decrease in productivity. Therefore, the objective of the study was to isolate rhizospheric actinobacteria from soil samples treated by physical methods and evaluate the inhibitory activity of the isolates over Xanthomonas. Initially, soil samples collected from avocado tree orchards were treated by dry heat air and microwave irradiation; thereafter, isolation was implemented. Then, antibacterial activity (AA) of isolates was evaluated by the double-layer agar method. Furthermore, the positive/negative effect on AA for selected isolates was evaluated on three culture media (potato-dextrose agar, PDA; yeast malt extract agar, YME; and oat agar, OA). Isolates were identified by 16S rRNA sequence analysis. A total of 198 isolates were obtained; 76 (series BVEZ) correspond to samples treated by dry heat and 122 strains (series BVEZMW) were isolated from samples irradiated with microwaves. A total of 19 dry heat and 25 microwave-irradiated isolates showed AA with inhibition zones (IZ, diameter in mm) ranging from 12.7 to 82.3 mm and from 11.4 to 55.4 mm, respectively. An increment for the AA was registered for isolates cultured on PDA and YME, with an IZ from 21.1 to 80.2 mm and 14.1 to 69.6 mm, respectively. A lower AA was detected when isolates were cultured on OA media (15.0 to 38.1 mm). Based on the 16S rRNA gene sequencing analysis, the actinobacteria belong to the Streptomyces (6) and Amycolatopsis (2) genera. Therefore, the study showed that microwave irradiation is a suitable method to increase the isolation of soil bacteria with AA against Xanthomonas sp. In addition, Streptomyces sp. BVEZ 50 was the isolate with the highest IZ (80.2 mm). Full article

With the development of offshore oil fields, the reservoirs of X oilfield in the west of the South China Sea are poor in physical property, serious in pollution, and increasingly prominent in interlayer contradictions. Water injection dilation technology has strongly affected the development of loose sandstone reservoirs. To explore whether this technology applies to the low-permeability sandstone of X oilfield in the west of the South China Sea and the dilation effect and radius of water injection dilation technology on the target reservoir, low confining pressure rock mechanics experiments and numerical simulation of water injection in this reservoir section are carried out. The triaxial shear experiment of low confining pressure shows that the target reservoir sandstone with low-permeability can have a shear strength of 45 MPa when the effective confining pressure is 0.5 MPa, and the target reservoir core can have dilatancy. When the axial strain is 2.5%, the core dilatancy is 1%, and the permeability changes by 1.17 times. It was found that the core volume dilation was obviously under low effective confining pressure, and the permeability is 2 orders higher than in the initial condition. The numerical simulation of the target reservoir shows that the bottom-hole pressure reaches 47.12 MPa at the end of water injection in typical wells. The reservoir was deformed to different degrees around the well, and the top layer was raised by 5.58 mm. This paper characterizes the rock expansion potential and expansion flow capacity of low-permeability sandstone reservoirs from multiple perspectives and establishes a three-dimensional, full-size wellbore formation crustal stress strict matching geological model for offshore expansion wells. We have provided theoretical guidance for on-site construction. Full article

The risk of infections in chronic wounds represents a serious issue, particularly in aged people and in patients affected by diseases such as diabetes and obesity. Moreover, the growing resistance demonstrated by many bacterial strains has significantly reduced the therapeutic options for clinicians and has become a great challenge for the researchers in the definition of novel approaches that promote the wound healing process and reduce the healing time. Tissue engineering approaches based on biomaterials and three-dimensional scaffolds have demonstrated huge potential in supporting cell proliferation; among them, Bombyx mori-derived silk fibroin is a very appealing possibility for the development of devices with regenerative properties for wound healing applications. However, due to the high risk of infections in chronic wounds, an antibacterial treatment is also strongly encouraged for preventing bacterial proliferation at the wound site. In this work, to develop a device with regenerative and antibacterial properties, antibacterial silver coatings were deposited onto silk fibroin scaffolds, and the effect of the treatment in terms of chemical–physical and microbiological properties was investigated. The results demonstrated that the silver treatment improved the mechanical properties of the protein scaffold and provided good antibacterial efficacy against representative bacterial strains in wound infection, namely Escherichia coli and antibiotic-resistant Pseudomonas aeruginosa. Full article

Two-dimensional (2D) semiconductor components have excellent physical attributes, such as excellent mechanical ductility, high mobility, low dielectric constant, and tunable bandgap, which have attracted much attention to the fields of flexible devices, optoelectronic conversion, and microelectronic devices. Additionally, one-dimensional (1D) semiconductor materials with unique physical attributes, such as high surface area and mechanical potency, show great potential in many applications. However, isolated 1D and 2D materials often do not meet the demand for multifunctionality. Therefore, more functionality is achieved by reconstructing new composite structures from 1D and 2D materials, and according to the current study, it has been demonstrated that hybrid dimensional integration yields a significant enhancement in performance and functionality, which is widely promising in the field of constructing novel electronic and optoelectronic nanodevices. In this review, we first briefly introduce the preparation methods of 1D materials, 2D materials, and 1D/2D heterostructures, as well as their advantages and limitations. The applications of 1D/2D heterostructures in photodetectors, gas sensors, pressure and strain sensors, as well as photoelectrical synapses and biosensors are then discussed, along with the opportunities and challenges of their current applications. Finally, the outlook of the emerging field of 1D/2D heterojunction structures is given. Full article

This study explores the water consumption and greenhouse gas (GHG) emissions in the Yarkand River Basin, focusing on their dynamic interactions across industrial sectors. Utilizing environmental input–output analysis (IOA), the CROPWAT model, and life cycle assessment (LCA), we quantified the historical evolution of physical and virtual water cycles in relation to the water–carbon nexus. Our findings reveal that the planting industry, particularly the production of export-oriented, water-intensive crops like cotton, significantly contributes to both blue and green water consumption, exacerbating regional water scarcity. The persistent external market demand drives this over-extraction, further strained by the basin’s limited water retention capabilities. Although advancements have been made in reducing the per-unit water footprint of crops, total water consumption continues to rise due to agricultural expansion, intensifying pressure on blue water resources. Additionally, agricultural GHG emissions have surged, driven by increased electricity consumption, heavy fertilizer use, and escalating soil N₂O emissions. In light of these challenges, our research underscores the critical need for integrated resource management strategies that align with sustainable development goals. By promoting efficient water allocation within the agricultural sector and diversifying crop structures downstream, we can enhance ecosystem resilience and reduce environmental degradation. Furthermore, the advancement of value-added agricultural processing and the implementation of innovative water conservation technologies are essential for fostering economic sustainability. These strategies not only mitigate the environmental impacts associated with agricultural practices but also strengthen the region’s adaptive capacity in the face of climate change and fluctuating market demands. Our findings contribute to the broader discourse on sustainable agricultural practices, emphasizing the interconnectedness of water management, climate resilience, and economic viability in arid regions. Full article

Background/Objectives: The issue of effective wound healing remains highly relevant. The objective of the study is to develop an optimal method for the synthesis of nanosized cerium oxide powder obtained via the thermal decomposition of cerium carbonate precipitated from aqueous nitrate solution for the technical creation of new drugs in production conditions; the select modification of synthesis under different conditions based on the evaluation of the physicochemical characteristics of the obtained material and its biological activity, and an evaluation of the broad-spectrum effect on cells involved in the regeneration of skin structure as well as antimicrobial properties. Methods: Several modes of the industrial synthesis of cerium dioxide nanoparticles (NPs) were carried out. The synthesis stages and the chemical and physical parameters of the obtained NPs were described using transmission electron microscopy (TEM), X-ray diffraction, Raman spectroscopy, and mass spectrometry. The cell cultures of human fibroblasts and keratinocytes were cultured with different concentrations of different nanoceria variations, and the cytotoxicity and the metabolic and proliferative activity were investigated. An MTT test and cell counting were performed. The antimicrobial activity of CeO₂ variations at a concentration of 0.1–0.0001 M against Pseudomonas aeruginosa was studied. Results: The purity of the synthesized nanoceria powders in all the batches was >99.99%. According to TEM data, the size of the NPs varied from 1 nm to 70 nm under different conditions and methodologies. The most optimal technology for the synthesis of the nanoceria with the maximum biological effect was selected. A method for obtaining the most bioactive NPs of optimal size (up to 10 nm) was proposed. The repeatability of the results of the proposed method of nanoceria synthesis in terms of particle size was confirmed. It was proven that the more structural defects on the surface of the CeO₂ crystal lattice, the higher the efficiency of the NPs due to oxygen vacancies. The strain provided the best redox activity and antioxidant properties of the nanoceria, which was demonstrated by better regenerative potential on various cell lines. The beneficial effect of synthesized nanoceria on the proliferative and metabolic activity of the cell lines involved in skin regeneration (human fibroblasts, human keratinocytes) was demonstrated. The antimicrobial effect of synthesized nanoceria on the culture of the most-resistant-to-modern-antibiotics microorganism Pseudomonas aeruginosa was confirmed. The optimal concentrations of the nanoceria to achieve the maximum biological effect were determined (10⁻³ M). Conclusions: It was possible to develop a method for the industrial synthesis of nanoceria, which can be used to produce drugs and medical devices containing CeO₂ NPs. Full article

The mining industry is undergoing a transformation driven by the adoption of Industry 4.0 technologies, implementing autonomous trucks, drones, positions systems, and similar technologies. This article, drawing on experiences and observations from several studies conducted in the mining industry, explores the impact of these technologies on mining work. It identifies eight key potential changes in working conditions. Firstly, routine and dangerous tasks are increasingly automated, reducing physical strain but potentially leading to job displacement and increased maintenance demands. Secondly, operators and managers are shifting toward handling disturbances and training algorithms, as AI takes over decision-making processes. Thirdly, managers are responsible for more capital with fewer people, potentially altering managerial roles and spans of control. Fourthly, the global connectivity of operations makes the world both larger and smaller, with a universal language blurring boundaries. Fifthly, work becomes location-independent, allowing for remote operation and management. Sixthly, the distinction between work and private life blurs, with increased availability expected from operators and managers. Seventhly, technology expands human senses, providing real-time data and situational awareness. Eighthly and lastly, the pervasive collection and retention of data create a scenario where one’s history is inescapable, raising concerns about data ownership and privacy. These changes necessitate a strategic response from the mining industry to ensure socially sustainable technology development and to attract a future workforce. Full article

The diversity of the microbial community structure plays a crucial role in the flavor and nutritional value of Shanxi aged vinegar in fermentation. Illumina Miseq high-throughput sequencing identified thirteen bacterial genera, with Lactobacillales (44.89%) and Acetobacter (21.04%) being the predominant species. Meanwhile, the fermentation characteristics of selected lactic acid bacteria strains isolated from Shanxi aged vinegar were studied in different media. The results showed that the biomass, and physical and chemical indices, as well as flavor compounds of the four strains of lactic acid bacteria in the simulated vinegar fermented grains medium were superior to those in barley and pea medium and sorghum juice medium. The bacterial interaction was conducted to investigate the effects on growth, the physicochemical indices, and flavor substances in order to determine the optimal combination. Furthermore, the interaction between pure cultures and co-cultures of lactic acid bacteria in a simulated vinegar culture medium was investigated, with a focus on the impact of this interaction on strain growth, fermentation characteristics, and flavor compound production. Compared with the pure culture, when strains L7 and L729 were co-inoculated, the reducing sugar content was 0.31 ± 0.01 g/100 g, total acid content was 3.02 ± 0.06 g/100 g, acetoin content was 2.41 ± 0.07 g/100 g, and total organic acid content was 3.77 ± 0.17 g/100 g. In terms of flavor compounds, the combined culture system exhibited higher levels of esters, aldehydes, and acids compared to pure cultures or other co-culture systems. This study revealed the fermentation characteristics of selected lactic acid strains in Shanxi aged vinegar under different conditions and their interaction in simulated vinegar fermentation media, which could provide theoretical support for the safety and health evaluation of aged vinegar. Full article