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22 pages, 10421 KiB  
Article
Distributed High-Speed Videogrammetry for Real-Time 3D Displacement Monitoring of Large Structure on Shaking Table
by Haibo Shi, Peng Chen, Xianglei Liu, Zhonghua Hong, Zhen Ye, Yi Gao, Ziqi Liu and Xiaohua Tong
Remote Sens. 2024, 16(23), 4345; https://doi.org/10.3390/rs16234345 - 21 Nov 2024
Abstract
The accurate and timely acquisition of high-frequency three-dimensional (3D) displacement responses of large structures is crucial for evaluating their condition during seismic excitation on shaking tables. This paper presents a distributed high-speed videogrammetric method designed to rapidly measure the 3D displacement of large [...] Read more.
The accurate and timely acquisition of high-frequency three-dimensional (3D) displacement responses of large structures is crucial for evaluating their condition during seismic excitation on shaking tables. This paper presents a distributed high-speed videogrammetric method designed to rapidly measure the 3D displacement of large shaking table structures at high sampling frequencies. The method uses non-coded circular targets affixed to key points on the structure and an automatic correspondence approach to efficiently estimate the extrinsic parameters of multiple cameras with large fields of view. This process eliminates the need for large calibration boards or manual visual adjustments. A distributed computation and reconstruction strategy, employing the alternating direction method of multipliers, enables the global reconstruction of time-sequenced 3D coordinates for all points of interest across multiple devices simultaneously. The accuracy and efficiency of this method were validated through comparisons with total stations, contact sensors, and conventional approaches in shaking table tests involving large structures with RCBs. Additionally, the proposed method demonstrated a speed increase of at least six times compared to the advanced commercial photogrammetric software. It could acquire 3D displacement responses of large structures at high sampling frequencies in real time without requiring a high-performance computing cluster. Full article
Show Figures

Figure 1

Figure 1
<p>Framework of the proposed videogrammetric method.</p>
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<p>General distributed videogrammetric network.</p>
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<p>Stereo-matching method of circular targets in large FOV (red dots indicate SIFT feature points of stereo images).</p>
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<p>Distributed computation and reconstruction strategy.</p>
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<p>(<b>a</b>) Real structure model. (<b>b</b>) Camera layout and spatial coordinate system. (<b>c</b>) Measurement point distribution.</p>
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<p>Measurement errors between the videogrammetry and the total station at each checkpoint in the X, Y, and Z directions.</p>
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<p>Three-dimensional positioning errors of the checkpoint calculated using different methods after each seismic wave load.</p>
Full article ">Figure 8
<p>Comparison of displacement and acceleration response histories obtained by the proposed videogrammetry and contact sensors at points <span class="html-italic">R</span><sub>3</sub> and <span class="html-italic">R</span><sub>18</sub> subjected to different seismic excitations: (<b>a</b>) Experiment No. 1; (<b>b</b>) Experiment No. 3; (<b>c</b>) Experiment No. 5.</p>
Full article ">Figure 9
<p>Time consumption and mean reprojection error of different methods for reconstructing the shaking table dataset.</p>
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<p>Time consumption of different methods for reconstructing the shaking table dataset.</p>
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<p>Three-dimensional displacement response histories of measurement points distributed across the coupling beams during (<b>a</b>) Experiment No. 1, (<b>b</b>) Experiment No. 3, and (<b>c</b>) Experiment No. 5.</p>
Full article ">
22 pages, 14579 KiB  
Article
Towards Effective Participation: A Stakeholder-Centered Evaluation for Aggregating Spatially Explicit Data
by Sebastian Leahy and Eduardo Dias
Land 2024, 13(12), 1973; https://doi.org/10.3390/land13121973 - 21 Nov 2024
Viewed by 157
Abstract
As participatory mapping becomes more ingrained into participatory practices, it is vital to understand what makes a method effective. Through the employment of a participatory mapping experiment within an ongoing urban greening initiative, this research aims at validating two established aggregation methods of [...] Read more.
As participatory mapping becomes more ingrained into participatory practices, it is vital to understand what makes a method effective. Through the employment of a participatory mapping experiment within an ongoing urban greening initiative, this research aims at validating two established aggregation methods of spatially explicit data points in participatory mapping. Utilizing a paper mapping activity with community residents, the collected spatial data points were analyzed using both democratic and weighted aggregation. Subsequently, a follow-up study was employed to validate the perceived feeling of ownership among both of the methods in terms of the stakeholders’ sense of community representation within the visual outputs. This research introduces a novel approach to the evaluation of participatory mapping practices. A key finding includes the development of a comprehensive methodological model that explores various aspects of a participatory process. Additionally, a new criterion was presented, stakeholder representation, in which to evaluate effectiveness. The difference between the methods was not found to be significant, as the mapping results revealed a consistent overlap in the areas of interest across the stakeholder groups, suggesting a general public consensus on the local development priorities. These insights hold considerable value for local decisions makers, as agreement areas provide a stable foundation for planning. Lastly, this research contributes to the ongoing efforts to evaluate and standardize participatory mapping methods, while also addressing the potential of varied aggregation methods to enhance effectiveness. Full article
(This article belongs to the Special Issue Geodesign in Urban Planning)
Show Figures

Figure 1

Figure 1
<p>(<b>a</b>) Map of Science Park boundary (red) and Carolina Macgillavrylaan (green); (<b>b</b>) Map of Science Park within the surrounding city of Amsterdam.</p>
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<p>Two images captured from the center of Carolina Macgillavrylaan (<b>a</b>) facing north-west; (<b>b</b>) facing south-east.</p>
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<p>(<b>a</b>) Picture of resident mind mapping activity; (<b>b</b>) picture of stakeholder meeting.</p>
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<p>The final mapping survey worksheet.</p>
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<p>Summary of GIS processes.</p>
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<p>Bar graphs of descriptive statistics; (<b>a</b>) problems with Carolina Macgillavrylaan; (<b>b</b>) stakeholder satisfaction with current street structure.</p>
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<p>(<b>a</b>) Example of spatially explicit data drawn by a resident; (<b>b</b>) Example of spatially explicit data drawn by a student.</p>
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<p>Mapping activity visual results: (<b>a</b>) column of democratic aggregation for location preferences; (<b>b</b>) column of weighted aggregation for location preferences.</p>
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<p>Example of varying visualization methods (<b>a</b>) heatmaps; (<b>b</b>) overlapping polygons.</p>
Full article ">Figure A1
<p>Mapping activity survey questions including ranking schematic.</p>
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26 pages, 2492 KiB  
Article
The Effects of Tree Shade on Vineyard Microclimate and Grape Production: A Novel Approach to Sun Radiation Modelling as a Response to Climate Change
by Isilda Cunha Menezes, Mário Santos, Lourdes Bugalho and Mário Gonzalez Pereira
Land 2024, 13(11), 1970; https://doi.org/10.3390/land13111970 - 20 Nov 2024
Viewed by 404
Abstract
Climate change threatens established agricultural systems and production, driving the need for adaptation and mitigation strategies. Vitiforestry, an alternative cultivation system combining trees and shrubs in the vineyard, promotes environmental sustainability and offers a possible adaptation strategy to climate change. This work scrutinizes [...] Read more.
Climate change threatens established agricultural systems and production, driving the need for adaptation and mitigation strategies. Vitiforestry, an alternative cultivation system combining trees and shrubs in the vineyard, promotes environmental sustainability and offers a possible adaptation strategy to climate change. This work scrutinizes the impact of shading on vineyards using an Integrated Model of Vineyard Shading and Climate Adaptation (IMVSCA), supported by a system dynamics approach. This model estimates solar radiation and computes daily and annual trends of insolation, air temperature, and relative humidity to shading and its influence on vineyard growth stages. It also assesses the effects of shading-related extreme weather events and the occurrence of grapevine disease development driven by daily weather conditions and zoning adaptations. The pilot results depict the effects of tree shading on vineyards, namely the impacts of solar radiation and air temperature on vine phenology, pollination, pollen germination, fungal diseases, and the complimentary indicators of grape production and quality. Our modeling framework and findings suggest that vitiforestry could be an interesting climate change adaptation technique, providing a starting point for further studies in this scope. Full article
(This article belongs to the Section Land–Climate Interactions)
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Figure 1

Figure 1
<p>The Integrated Model of Vineyard Shading and Climate Adaptation (IMVSCA) scheme, with the logical sequence of its modules. The Light–Shadow Module consists of three submodules: Sunrise–Sunset, Light–Orchard, and Tree–Shadow.</p>
Full article ">Figure 2
<p>Description of the shadow effect of a tree on a grapevine according to the (<b>a</b>) azimuthal and (<b>b</b>) zenithal movement of the sun. The tree has a cylindrical trunk of negligible diameter, a cylindrical crown with a radius <math display="inline"><semantics> <mrow> <mi>r</mi> </mrow> </semantics></math> and a height <math display="inline"><semantics> <mrow> <mi>h</mi> <mo>=</mo> <msub> <mrow> <mi>h</mi> </mrow> <mrow> <mi>c</mi> <mi>r</mi> <mi>o</mi> <mi>w</mi> <mi>n</mi> <mo> </mo> <mi>t</mi> <mi>o</mi> <mi>p</mi> </mrow> </msub> <mo>−</mo> <msub> <mrow> <mi>h</mi> </mrow> <mrow> <mi>c</mi> <mi>r</mi> <mi>o</mi> <mi>w</mi> <mi>n</mi> <mo> </mo> <mi>b</mi> <mi>a</mi> <mi>s</mi> <mi>e</mi> </mrow> </msub> </mrow> </semantics></math>. Without losing generality, in the case illustrated in the figure, the grapevine is located at point O and the tree at point T to the east, and at a distance (<math display="inline"><semantics> <mrow> <msub> <mrow> <mi>d</mi> </mrow> <mrow> <mi>V</mi> <mi>T</mi> </mrow> </msub> </mrow> </semantics></math>) from the grapevine. On panel (<b>a</b>), <math display="inline"><semantics> <mrow> <mi>ψ</mi> </mrow> </semantics></math> represents the azimuthal angle of the sun, which changes from sunrise to sunset, <math display="inline"><semantics> <mrow> <msub> <mrow> <mi>α</mi> </mrow> <mrow> <mi>T</mi> </mrow> </msub> </mrow> </semantics></math> is the constant azimuth angle of the tree trunk, and <math display="inline"><semantics> <mrow> <mi>β</mi> </mrow> </semantics></math> is the shadow angle defined between the line segments <math display="inline"><semantics> <mrow> <mover accent="true"> <mrow> <mi>O</mi> <mi>T</mi> </mrow> <mo>¯</mo> </mover> </mrow> </semantics></math> and the tangents <math display="inline"><semantics> <mrow> <mi>c</mi> </mrow> </semantics></math> to the projection of the crown on the horizontal plane. On panel (<b>b</b>), <math display="inline"><semantics> <mrow> <mi>θ</mi> </mrow> </semantics></math> is the solar height angle, which also changes in time, and <math display="inline"><semantics> <mrow> <msub> <mrow> <mi>θ</mi> </mrow> <mrow> <mn>1</mn> </mrow> </msub> </mrow> </semantics></math> and <math display="inline"><semantics> <mrow> <msub> <mrow> <mi>θ</mi> </mrow> <mrow> <mn>2</mn> </mrow> </msub> </mrow> </semantics></math> are the height angle of the tree crown top and bottom, respectively.</p>
Full article ">Figure 3
<p>The monthly anomaly of the mean air temperature (<b>left panel</b>) and the precipitation (<b>right panel</b>) in the weather station of Pinhão for the years 1981–1982 and 2004–2005 (an anomaly is the difference between the monthly mean for a specific month of a specific year and the climatological normal for that month).</p>
Full article ">Figure 4
<p>Periods (maximum and minimum dates) of the major phenological phases of the grapevines grown in the Douro Valley (beige horizontal bars), computed for the 1980–2009 period (Real et al., 2015), and the average dates of the same events obtained from the observed phenological dates in the city of Peso da Régua (light pink vertical bars) provided by the Association for the Development of Douro Viticulture (Associação para o Desenvolvimento da Viticultura Duriense, ADVID) (ADVID 2012), and in the Quinta de Santa Bárbara (QSB) (dark pink vertical bars), located in Pinhão (Sousa 2014), and simulated by the PM, using data from the weather station located in Pinhão, for the Touriga Franca variety, years 1982 and 2005, and considering the shadow effect of trees placed at the distances of 2.5 m (T2.5m) and 3 m (T3m) from the grapevine, north, east, south, and west of the grapevine.</p>
Full article ">Figure 5
<p>Simulation of the days with optimum weather conditions for the grapevine pollination and germination of the pollen for the years 1982 and 2005, under the influence of the trees located at a distance from the vineyard of 2.5 m and 3 m positioned at the north, west or east, and south of the grapevine.</p>
Full article ">
22 pages, 6690 KiB  
Article
Effects of Differential Displacements Between the Ground Anchors in Suspension Bridges
by Paolo Clemente
Infrastructures 2024, 9(11), 211; https://doi.org/10.3390/infrastructures9110211 - 20 Nov 2024
Viewed by 230
Abstract
A simple model to evaluate the effects of relative displacements between the ground anchors of a suspension bridge is proposed. An equation system is defined, which allows for the evaluation of the structural response under a general displacement set of the ground anchor [...] Read more.
A simple model to evaluate the effects of relative displacements between the ground anchors of a suspension bridge is proposed. An equation system is defined, which allows for the evaluation of the structural response under a general displacement set of the ground anchor points. Then, the most interesting and likely cases are analyzed in detail with reference to a suspension bridge having geometrical and mechanical characteristics typical of a long-span bridge. A simple procedure for the assessment of variation in cable stress is also given, which can be used to choose the optimum values for stress in cables under dead loads, as a percentage of their strength. The results obtained showed that expected movements do not significantly impact the structure in its lifetime and that the effects become negligible for very long-span bridges. Finally, the results obtained can be easily used for the condition monitoring of suspension bridges. Full article
(This article belongs to the Section Infrastructures and Structural Engineering)
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Figure 1

Figure 1
<p>General model of a suspension bridge.</p>
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<p>New configuration: longitudinal view and plan view.</p>
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<p>Details of angles between the new planes at (<b>a</b>) pylon B and (<b>b</b>) pylon C.</p>
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<p>Investigated suspension bridge model.</p>
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<p>Effects due to <math display="inline"><semantics> <mrow> <msub> <mover accent="true"> <mi>d</mi> <mo stretchy="false">^</mo> </mover> <mrow> <mi>D</mi> <mi>x</mi> </mrow> </msub> </mrow> </semantics></math>≠ 0.</p>
Full article ">Figure 6
<p>Variation in (<b>a</b>) <math display="inline"><semantics> <mrow> <msub> <mover accent="true"> <mi>f</mi> <mo stretchy="false">^</mo> </mover> <mn>1</mn> </msub> </mrow> </semantics></math> and (<b>b</b>) <math display="inline"><semantics> <mrow> <msub> <mover accent="true"> <mi>H</mi> <mo stretchy="false">^</mo> </mover> <mn>1</mn> </msub> </mrow> </semantics></math> due to <math display="inline"><semantics> <mrow> <msub> <mover accent="true"> <mi>d</mi> <mo stretchy="false">^</mo> </mover> <mrow> <mi>D</mi> <mi>x</mi> </mrow> </msub> </mrow> </semantics></math> ≠ 0.</p>
Full article ">Figure 7
<p>Effects of <math display="inline"><semantics> <mrow> <msub> <mover accent="true"> <mi>d</mi> <mo stretchy="false">^</mo> </mover> <mrow> <mi>C</mi> <mi>x</mi> </mrow> </msub> <mo>=</mo> <msub> <mover accent="true"> <mi>d</mi> <mo stretchy="false">^</mo> </mover> <mrow> <mi>D</mi> <mi>x</mi> </mrow> </msub> </mrow> </semantics></math> ≠ 0.</p>
Full article ">Figure 8
<p>Variation in (<b>a</b>) <math display="inline"><semantics> <mrow> <msub> <mover accent="true"> <mi>f</mi> <mo stretchy="false">^</mo> </mover> <mn>1</mn> </msub> </mrow> </semantics></math> and (<b>b</b>) <math display="inline"><semantics> <mrow> <msub> <mover accent="true"> <mi>H</mi> <mo stretchy="false">^</mo> </mover> <mn>1</mn> </msub> </mrow> </semantics></math> due to <math display="inline"><semantics> <mrow> <msub> <mover accent="true"> <mi>d</mi> <mo stretchy="false">^</mo> </mover> <mrow> <mi>C</mi> <mi>x</mi> </mrow> </msub> <mo>=</mo> <msub> <mover accent="true"> <mi>d</mi> <mo stretchy="false">^</mo> </mover> <mrow> <mi>D</mi> <mi>x</mi> </mrow> </msub> </mrow> </semantics></math> ≠ 0.</p>
Full article ">Figure 9
<p>Effects of <math display="inline"><semantics> <mrow> <msub> <mover accent="true"> <mi>d</mi> <mo stretchy="false">^</mo> </mover> <mrow> <mi>D</mi> <mi>z</mi> </mrow> </msub> </mrow> </semantics></math>≠ 0.</p>
Full article ">Figure 10
<p>Variation in (<b>a</b>) <math display="inline"><semantics> <mrow> <msub> <mover accent="true"> <mi>f</mi> <mo stretchy="false">^</mo> </mover> <mn>1</mn> </msub> </mrow> </semantics></math> and (<b>b</b>) <math display="inline"><semantics> <mrow> <msub> <mover accent="true"> <mi>H</mi> <mo stretchy="false">^</mo> </mover> <mn>1</mn> </msub> </mrow> </semantics></math> due to <math display="inline"><semantics> <mrow> <msub> <mover accent="true"> <mi>d</mi> <mo stretchy="false">^</mo> </mover> <mrow> <mi>D</mi> <mi>z</mi> </mrow> </msub> </mrow> </semantics></math> ≠ 0.</p>
Full article ">Figure 11
<p>Effects of <math display="inline"><semantics> <mrow> <msub> <mover accent="true"> <mi>d</mi> <mo stretchy="false">^</mo> </mover> <mrow> <mi>C</mi> <mi>z</mi> </mrow> </msub> <mo>=</mo> <msub> <mover accent="true"> <mi>d</mi> <mo stretchy="false">^</mo> </mover> <mrow> <mi>D</mi> <mi>z</mi> </mrow> </msub> </mrow> </semantics></math> ≠ 0.</p>
Full article ">Figure 12
<p>Variation in (<b>a</b>) <math display="inline"><semantics> <mrow> <msub> <mover accent="true"> <mi>f</mi> <mo stretchy="false">^</mo> </mover> <mn>1</mn> </msub> </mrow> </semantics></math> and (<b>b</b>) <math display="inline"><semantics> <mrow> <msub> <mover accent="true"> <mi>H</mi> <mo stretchy="false">^</mo> </mover> <mn>1</mn> </msub> </mrow> </semantics></math> due to <math display="inline"><semantics> <mrow> <msub> <mover accent="true"> <mi>d</mi> <mo stretchy="false">^</mo> </mover> <mrow> <mi>C</mi> <mi>z</mi> </mrow> </msub> <mo>=</mo> <msub> <mover accent="true"> <mi>d</mi> <mo stretchy="false">^</mo> </mover> <mrow> <mi>D</mi> <mi>z</mi> </mrow> </msub> </mrow> </semantics></math> ≠ 0.</p>
Full article ">Figure 13
<p>Effects of <span class="html-italic">d<sub>Dy</sub></span> ≠ 0.</p>
Full article ">Figure 14
<p>Variation in (<b>a</b>) <math display="inline"><semantics> <mrow> <msub> <mover accent="true"> <mi>f</mi> <mo stretchy="false">^</mo> </mover> <mn>1</mn> </msub> </mrow> </semantics></math>, (<b>b</b>) <math display="inline"><semantics> <mrow> <msub> <mover accent="true"> <mi>H</mi> <mo stretchy="false">^</mo> </mover> <mn>1</mn> </msub> </mrow> </semantics></math>, (<b>c</b>) <math display="inline"><semantics> <mrow> <msub> <mover accent="true"> <mi>H</mi> <mo stretchy="false">^</mo> </mover> <mrow> <mi>C</mi> <mi>y</mi> </mrow> </msub> </mrow> </semantics></math> and (<b>d</b>) <math display="inline"><semantics> <mrow> <msub> <mover accent="true"> <mi>H</mi> <mo stretchy="false">^</mo> </mover> <mrow> <mi>C</mi> <mi>x</mi> </mrow> </msub> </mrow> </semantics></math> due to <math display="inline"><semantics> <mrow> <msub> <mover accent="true"> <mi>d</mi> <mo stretchy="false">^</mo> </mover> <mrow> <mi>D</mi> <mi>y</mi> </mrow> </msub> </mrow> </semantics></math> ≠ 0.</p>
Full article ">Figure 15
<p>Effects of <span class="html-italic">d<sub>Cy</sub></span> = <span class="html-italic">d<sub>Dy</sub></span> ≠ 0.</p>
Full article ">Figure 16
<p>Variation in (<b>a</b>) <math display="inline"><semantics> <mrow> <msub> <mover accent="true"> <mi>f</mi> <mo stretchy="false">^</mo> </mover> <mn>1</mn> </msub> </mrow> </semantics></math>, (<b>b</b>) <math display="inline"><semantics> <mrow> <msub> <mover accent="true"> <mi>H</mi> <mo stretchy="false">^</mo> </mover> <mn>1</mn> </msub> </mrow> </semantics></math>, (<b>c</b>) <math display="inline"><semantics> <mrow> <msub> <mover accent="true"> <mi>H</mi> <mo stretchy="false">^</mo> </mover> <mrow> <mi>C</mi> <mi>y</mi> </mrow> </msub> </mrow> </semantics></math>, and (<b>d</b>) <math display="inline"><semantics> <mrow> <msub> <mover accent="true"> <mi>H</mi> <mo stretchy="false">^</mo> </mover> <mrow> <mi>C</mi> <mi>x</mi> </mrow> </msub> </mrow> </semantics></math> due to <math display="inline"><semantics> <mrow> <msub> <mover accent="true"> <mi>d</mi> <mo stretchy="false">^</mo> </mover> <mrow> <mi>C</mi> <mi>y</mi> </mrow> </msub> <mo>=</mo> <msub> <mover accent="true"> <mi>d</mi> <mo stretchy="false">^</mo> </mover> <mrow> <mi>D</mi> <mi>y</mi> </mrow> </msub> </mrow> </semantics></math> ≠ 0.</p>
Full article ">Figure 17
<p>Effects of tower rotation β<sub>B</sub>.</p>
Full article ">Figure 18
<p>Variation in (<b>a</b>) <math display="inline"><semantics> <mrow> <msub> <mover accent="true"> <mi>f</mi> <mo stretchy="false">^</mo> </mover> <mn>1</mn> </msub> </mrow> </semantics></math> and (<b>b</b>) <math display="inline"><semantics> <mrow> <msub> <mover accent="true"> <mi>H</mi> <mo stretchy="false">^</mo> </mover> <mn>1</mn> </msub> </mrow> </semantics></math> due to <math display="inline"><semantics> <mrow> <msub> <mi>β</mi> <mi>B</mi> </msub> </mrow> </semantics></math> ≠ 0.</p>
Full article ">Figure 19
<p>Variation in α<sub>1</sub> due to (<b>a</b>) <math display="inline"><semantics> <mrow> <msub> <mover accent="true"> <mi>d</mi> <mo stretchy="false">^</mo> </mover> <mrow> <mi>D</mi> <mi>x</mi> </mrow> </msub> </mrow> </semantics></math> ≠ 0 and (<b>b</b>) to <math display="inline"><semantics> <mrow> <msub> <mover accent="true"> <mi>d</mi> <mo stretchy="false">^</mo> </mover> <mrow> <mi>C</mi> <mi>x</mi> </mrow> </msub> <mo>=</mo> <msub> <mover accent="true"> <mi>d</mi> <mo stretchy="false">^</mo> </mover> <mrow> <mi>D</mi> <mi>x</mi> </mrow> </msub> </mrow> </semantics></math> ≠ 0.</p>
Full article ">Figure 20
<p>Variation in α<sub>1</sub> due to (<b>a</b>) <math display="inline"><semantics> <mrow> <msub> <mover accent="true"> <mi>d</mi> <mo stretchy="false">^</mo> </mover> <mrow> <mi>C</mi> <mi>z</mi> </mrow> </msub> </mrow> </semantics></math>≠ 0 and (<b>b</b>) to <math display="inline"><semantics> <mrow> <msub> <mover accent="true"> <mi>d</mi> <mo stretchy="false">^</mo> </mover> <mrow> <mi>C</mi> <mi>z</mi> </mrow> </msub> <mo>=</mo> <msub> <mover accent="true"> <mi>d</mi> <mo stretchy="false">^</mo> </mover> <mrow> <mi>D</mi> <mi>z</mi> </mrow> </msub> </mrow> </semantics></math> ≠ 0.</p>
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<p>Variation in α<sub>1</sub> due to (<b>a</b>) <math display="inline"><semantics> <mrow> <msub> <mover accent="true"> <mi>d</mi> <mo stretchy="false">^</mo> </mover> <mrow> <mi>C</mi> <mi>y</mi> </mrow> </msub> </mrow> </semantics></math> ≠ 0 and (<b>b</b>) to <math display="inline"><semantics> <mrow> <msub> <mover accent="true"> <mi>d</mi> <mo stretchy="false">^</mo> </mover> <mrow> <mi>C</mi> <mi>y</mi> </mrow> </msub> <mo>=</mo> <msub> <mover accent="true"> <mi>d</mi> <mo stretchy="false">^</mo> </mover> <mrow> <mi>D</mi> <mi>y</mi> </mrow> </msub> </mrow> </semantics></math> ≠ 0.</p>
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<p>Variation in α<sub>1</sub> due to <math display="inline"><semantics> <mrow> <msub> <mi>β</mi> <mi>B</mi> </msub> </mrow> </semantics></math> ≠ 0.</p>
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<p>Variation in α<sub>1</sub> due to <math display="inline"><semantics> <mrow> <msub> <mover accent="true"> <mi>d</mi> <mo stretchy="false">^</mo> </mover> <mrow> <mi>C</mi> <mi>x</mi> </mrow> </msub> <mo>=</mo> <msub> <mover accent="true"> <mi>d</mi> <mo stretchy="false">^</mo> </mover> <mrow> <mi>D</mi> <mi>x</mi> </mrow> </msub> <mo>=</mo> <msub> <mover accent="true"> <mi>d</mi> <mo stretchy="false">^</mo> </mover> <mrow> <mi>D</mi> <mi>z</mi> </mrow> </msub> </mrow> </semantics></math>.</p>
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20 pages, 2942 KiB  
Systematic Review
Natural Antibacterial Compounds with Potential for Incorporation into Dental Adhesives: A Systematic Review
by Ana Catarina Sousa, Paulo Mascarenhas, Mário Polido and Joana Vasconcelos e Cruz
Polymers 2024, 16(22), 3217; https://doi.org/10.3390/polym16223217 - 20 Nov 2024
Viewed by 236
Abstract
Dental adhesives are essential in modern restorative dentistry and are constantly evolving. However, challenges like secondary caries from bacterial infiltration at the adhesive–tooth interface persist. While synthetic antibacterial agents in adhesives show promise, safety concerns have shifted interest toward natural options that are [...] Read more.
Dental adhesives are essential in modern restorative dentistry and are constantly evolving. However, challenges like secondary caries from bacterial infiltration at the adhesive–tooth interface persist. While synthetic antibacterial agents in adhesives show promise, safety concerns have shifted interest toward natural options that are biocompatible, sustainable, and effective. Therefore, this study evaluated whether natural antibacterial compounds in dental adhesives can provide effective antimicrobial activity without compromising their integrity. This systematic review followed PRISMA 2020 statement guidelines. Four databases were screened, PubMed, Scopus, EMBASE, and Web of Science, without language or publication date restrictions until July 2024. The selection criteria were in vitro studies in which natural antimicrobial substances were incorporated into dental adhesives and the resulting composites were tested for their antibacterial and physicochemical properties. A quality assessment was conducted on the selected studies. Most of the studies reviewed reported significant antibacterial activity while retaining the adhesive’s integrity, generally achieved with lower concentrations of the natural agents. Higher concentrations increase the antimicrobial effectiveness but negatively impact the adhesive’s properties. This review highlights the promising role of natural antibacterial compounds in enhancing the functionality of dental adhesives while also pointing to the need for continued research to address current challenges. Full article
(This article belongs to the Special Issue Advanced Polymer-Based Materials for Dental Applications)
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<p>Flowchart outlining the study selection process (as described in the PRISMA 2020 statement guidelines).</p>
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<p>Traffic light plot of the studies’ risk of bias (RoB) analysis [<a href="#B18-polymers-16-03217" class="html-bibr">18</a>,<a href="#B35-polymers-16-03217" class="html-bibr">35</a>,<a href="#B36-polymers-16-03217" class="html-bibr">36</a>,<a href="#B37-polymers-16-03217" class="html-bibr">37</a>,<a href="#B38-polymers-16-03217" class="html-bibr">38</a>,<a href="#B39-polymers-16-03217" class="html-bibr">39</a>,<a href="#B40-polymers-16-03217" class="html-bibr">40</a>,<a href="#B41-polymers-16-03217" class="html-bibr">41</a>,<a href="#B42-polymers-16-03217" class="html-bibr">42</a>,<a href="#B43-polymers-16-03217" class="html-bibr">43</a>,<a href="#B44-polymers-16-03217" class="html-bibr">44</a>,<a href="#B45-polymers-16-03217" class="html-bibr">45</a>,<a href="#B46-polymers-16-03217" class="html-bibr">46</a>].</p>
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<p>Summary plot of the studies’ risk of bias (RoB) analysis.</p>
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23 pages, 32729 KiB  
Article
PLC-Fusion: Perspective-Based Hierarchical and Deep LiDAR Camera Fusion for 3D Object Detection in Autonomous Vehicles
by Husnain Mushtaq, Xiaoheng Deng, Fizza Azhar, Mubashir Ali and Hafiz Husnain Raza Sherazi
Information 2024, 15(11), 739; https://doi.org/10.3390/info15110739 - 19 Nov 2024
Viewed by 202
Abstract
Accurate 3D object detection is essential for autonomous driving, yet traditional LiDAR models often struggle with sparse point clouds. We propose perspective-aware hierarchical vision transformer-based LiDAR-camera fusion (PLC-Fusion) for 3D object detection to address this. This efficient, multi-modal 3D object detection framework integrates [...] Read more.
Accurate 3D object detection is essential for autonomous driving, yet traditional LiDAR models often struggle with sparse point clouds. We propose perspective-aware hierarchical vision transformer-based LiDAR-camera fusion (PLC-Fusion) for 3D object detection to address this. This efficient, multi-modal 3D object detection framework integrates LiDAR and camera data for improved performance. First, our method enhances LiDAR data by projecting them onto a 2D plane, enabling the extraction of object perspective features from a probability map via the Object Perspective Sampling (OPS) module. It incorporates a lightweight perspective detector, consisting of interconnected 2D and monocular 3D sub-networks, to extract image features and generate object perspective proposals by predicting and refining top-scored 3D candidates. Second, it leverages two independent transformers—CamViT for 2D image features and LidViT for 3D point cloud features. These ViT-based representations are fused via the Cross-Fusion module for hierarchical and deep representation learning, improving performance and computational efficiency. These mechanisms enhance the utilization of semantic features in a region of interest (ROI) to obtain more representative point features, leading to a more effective fusion of information from both LiDAR and camera sources. PLC-Fusion outperforms existing methods, achieving a mean average precision (mAP) of 83.52% and 90.37% for 3D and BEV detection, respectively. Moreover, PLC-Fusion maintains a competitive inference time of 0.18 s. Our model addresses computational bottlenecks by eliminating the need for dense BEV searches and global attention mechanisms while improving detection range and precision. Full article
(This article belongs to the Special Issue Emerging Research in Object Tracking and Image Segmentation)
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<p>The architecture of our PLC-Fusion model for 3D object detection using LiDAR and camera data. The raw point cloud from LiDAR and raw image data are processed by separate 3D and 2D backbones, respectively. Perspective-based sampling is applied to both modalities before passing through a vision transformer (ViT)-based model (LiDViT for LiDAR data and CamViT for image data) to establish 2D and 3D correspondence. The Cross-Fusion module integrates these features, followed by region of interest (RoI)-based 3D detection for generating 3D bounding box predictions.</p>
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<p>Graphical depiction of the object perspective sampling process for LiDAR and camera data within the multimodal fusion model.</p>
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<p>Illustration of our object perspective sampling and projection process for LiDAR and camera data within the multimodal fusion model. The sampled points from LiDAR and camera images are projected into their respective 3D and 2D coordinate systems. Sparse feature extraction is applied to both modalities before being passed into vision transformer (ViT)-based encoders (LiDAR-ViT for LiDAR features and camera-ViT for image features). These extracted features are then fused in the Cross-Fusion module to establish a 2D–3D correspondence for improved multimodal 3D object detection.</p>
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<p>The figure illustrates the vision transformer (ViT)-based cross-fusion approach for 3D object detection, combining camera and LiDAR data. Object perspective sampling extracts features from both sensors. The camera branch (CamViT) generates 3D and 2D feature maps <math display="inline"><semantics> <mrow> <msub> <mi mathvariant="bold">H</mi> <mi>c</mi> </msub> <mo>∈</mo> <msup> <mi mathvariant="double-struck">R</mi> <mrow> <msub> <mi>N</mi> <mi>c</mi> </msub> <mo>×</mo> <msub> <mi>D</mi> <mi>c</mi> </msub> </mrow> </msup> </mrow> </semantics></math> using multi-head attention (MH-Attention) and a feedforward neural network (FFN), while the LiDAR branch (LiDViT) processes 3D voxel features <math display="inline"><semantics> <mrow> <msub> <mi mathvariant="bold">H</mi> <mi>v</mi> </msub> <mo>∈</mo> <msup> <mi mathvariant="double-struck">R</mi> <mrow> <mi>V</mi> <mo>×</mo> <msub> <mi>D</mi> <mi>v</mi> </msub> </mrow> </msup> </mrow> </semantics></math> through a similar transformer architecture. The 2D and 3D feature maps from both modalities are concatenated <math display="inline"><semantics> <mrow> <msub> <mi mathvariant="bold">F</mi> <mrow> <mi>f</mi> <mi>u</mi> <mi>s</mi> <mi>i</mi> <mi>o</mi> <mi>n</mi> </mrow> </msub> <mo>=</mo> <mrow> <mo>[</mo> <msub> <mi mathvariant="bold">A</mi> <mrow> <mi>c</mi> <mi>v</mi> </mrow> </msub> <mo>;</mo> <msub> <mi mathvariant="bold">H</mi> <mi>c</mi> </msub> <mo>;</mo> <msub> <mi mathvariant="bold">A</mi> <mrow> <mi>v</mi> <mi>c</mi> </mrow> </msub> <mo>;</mo> <msub> <mi mathvariant="bold">H</mi> <mi>v</mi> </msub> <mo>]</mo> </mrow> </mrow> </semantics></math> and undergo cross-attention to align visual and geometric data. A final FFN refines the fused representation <math display="inline"><semantics> <mrow> <msub> <mi mathvariant="bold">F</mi> <mrow> <mi>f</mi> <mi>i</mi> <mi>n</mi> <mi>a</mi> <mi>l</mi> </mrow> </msub> <mo>=</mo> <mi>MLP</mi> <mrow> <mo>(</mo> <msub> <mi mathvariant="bold">F</mi> <mrow> <mi>f</mi> <mi>u</mi> <mi>s</mi> <mi>i</mi> <mi>o</mi> <mi>n</mi> </mrow> </msub> <mo>)</mo> </mrow> </mrow> </semantics></math>, providing deep multimodal features for accurate object detection in 3D space.</p>
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<p>Visual results of the proposed method on the KITTI validation dataset. For each case of sub-figures (<b>a</b>–<b>d</b>), the top row shows the visualization in the RGB image, and the bottom row displays the visualization in the LiDAR point cloud. Green represents the ground truth, and blue denotes the predicted outcomes.</p>
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<p>Visual results of the proposed method on the KITTI test and validation datasets. Row (<b>a</b>) presents the testing results, and row (<b>b</b>) displays the validation outcomes. The detection results demonstrate the effectiveness of our method, with the dotted circles highlighting the undetected instances caused by distance and heavy occlusion.</p>
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<p>Car class with Moderate condition: AP vs. IoU on KITII validation set.</p>
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<p>Comparative analysis of the runtime of our model with recent methods.</p>
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31 pages, 4747 KiB  
Article
Optimizing Forest Management: Balancing Environmental and Economic Goals Using Game Theory and Multi-Objective Approaches
by Neda Amiri and Soleiman Mohammadi Limaei
Forests 2024, 15(11), 2044; https://doi.org/10.3390/f15112044 - 19 Nov 2024
Viewed by 234
Abstract
Forests are complex ecosystems that require integrated management to balance economic, social, and environmental dimensions. Conflicting objectives among stakeholders make optimal decision-making particularly challenging. This study seeks to balance the economic gains of forest harvesting with the goals of environmental conservation, with a [...] Read more.
Forests are complex ecosystems that require integrated management to balance economic, social, and environmental dimensions. Conflicting objectives among stakeholders make optimal decision-making particularly challenging. This study seeks to balance the economic gains of forest harvesting with the goals of environmental conservation, with a focus on the Shafarood forest in Northern Iran. We applied multi-objective optimization and game theory to maximize the net present value (NPV) of forest harvesting while enhancing carbon sequestration. The research utilized data on stumpage prices, harvesting costs, tree density, volume per ha, growth rates, interest rates, carbon sequestration, and labour costs. Applying the epsilon-constraint method, we derived Pareto optimal solutions for a bi-objective model, and game theory was applied to negotiate between economic and environmental stakeholders. In the fifth round of bargaining, a Nash equilibrium was achieved between the two players. At this equilibrium point, the economic player achieved NPV from forest harvesting of 9001.884 (IRR 10,000/ha) and amount of carbon sequestration of 159.9383 tons/ha. Meanwhile, the environmental player achieved NPV from forest harvesting of 7861.248 (IRR 10,000/ha), along with a carbon sequestration of 159.9731 tons/ha. Results indicate significant trade-offs but reveal potential gains for both economic and environmental goals. These findings provide a robust framework for sustainable forest management and offer practical tools to support informed decision-making for diverse stakeholders. Full article
(This article belongs to the Special Issue Optimization of Forestry and Forest Supply Chain)
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<p>Study area, from left to right: Guilan province, Shafarood watershed, Bargah Zamin (District, 7).</p>
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<p>Game theory and multi-objective modeling for optimal forest management.</p>
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<p>Range of objective function variations for each player at stock level 1.</p>
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<p>Range of objective function variations for each player at stock level 2.</p>
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<p>Range of objective function variations for each player at stock level 3.</p>
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<p>Range of objective function variations for each player at stock level 4.</p>
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<p>Range of objective function variations for each player at stock level 5.</p>
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<p>Sensitivity of NPV to interest rate changes for optimal stock of 457 (m<sup>3</sup>/ha).</p>
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<p>Sensitivity of NPV to interest rate changes for optimal standing inventory of 457 (m<sup>3</sup>/ha).</p>
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<p>Pareto optimal frontier at stock level 1.</p>
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<p>Pareto optimal frontier at stock level 2.</p>
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<p>Pareto optimal frontier at stock level 3.</p>
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<p>Pareto optimal frontier at stock level 4.</p>
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<p>Pareto optimal frontier at forest stock level 5.</p>
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25 pages, 3907 KiB  
Article
Exploring Hydrogen–Diesel Dual Fuel Combustion in a Light-Duty Engine: A Numerical Investigation
by Francesco Scrignoli, Alfredo Maria Pisapia, Tommaso Savioli, Ezio Mancaruso, Enrico Mattarelli and Carlo Alberto Rinaldini
Energies 2024, 17(22), 5761; https://doi.org/10.3390/en17225761 - 18 Nov 2024
Viewed by 292
Abstract
Dual fuel combustion has gained attention as a cost-effective solution for reducing the pollutant emissions of internal combustion engines. The typical approach is combining a conventional high-reactivity fossil fuel (diesel fuel) with a sustainable low-reactivity fuel, such as bio-methane, ethanol, or green hydrogen. [...] Read more.
Dual fuel combustion has gained attention as a cost-effective solution for reducing the pollutant emissions of internal combustion engines. The typical approach is combining a conventional high-reactivity fossil fuel (diesel fuel) with a sustainable low-reactivity fuel, such as bio-methane, ethanol, or green hydrogen. The last one is particularly interesting, as in theory it produces only water and NOx when it burns. However, integrating hydrogen into stock diesel engines is far from trivial due to a number of theoretical and practical challenges, mainly related to the control of combustion at different loads and speeds. The use of 3D-CFD simulation, supported by experimental data, appears to be the most effective way to address these issues. This study investigates the hydrogen-diesel dual fuel concept implemented with minimum modifications in a light-duty diesel engine (2.8 L, 4-cylinder, direct injection with common rail), considering two operating points representing typical partial and full load conditions for a light commercial vehicle or an industrial engine. The numerical analysis explores the effects of progressively replacing diesel fuel with hydrogen, up to 80% of the total energy input. The goal is to assess how this substitution affects engine performance and combustion characteristics. The results show that a moderate hydrogen substitution improves brake thermal efficiency, while higher substitution rates present quite a severe challenge. To address these issues, the diesel fuel injection strategy is optimized under dual fuel operation. The research findings are promising, but they also indicate that further investigations are needed at high hydrogen substitution rates in order to exploit the potential of the concept. Full article
(This article belongs to the Special Issue Advances in Ignition Technology for Combustion Engines)
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<p>Cross section of a 60° sector of the combustion chamber, corresponding to one injector hole.</p>
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<p>Computational grid at TDC.</p>
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<p>Comparison between experimental and numerical in-cylinder pressure and AHRR: (<b>a</b>) 2400 rpm–10.5 bar BMEP (<b>b</b>) 4000 rpm–13.5 bar BMEP.</p>
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<p>Comparison between experimental and numerical in-cylinder pressure and AHRR of the single-cylinder, optically accessible H<sub>2</sub>-D DF engine.</p>
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<p>Diesel fuel injection strategy for diesel (black line) and H2-D DF operation with increasing H<sub>2</sub> energy share: (<b>a</b>) 2400 rpm–10.5 bar BMEP; (<b>b</b>) 4000 rpm–13.5 bar BMEP.</p>
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<p>Variation in the equivalence ratio of the H<sub>2</sub>-air premixed charge with increasing H<sub>2</sub> energy share at partial load (2400 rpm–10.5 bar BMEP).</p>
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<p>Effect of increasing H<sub>2</sub> energy share on in-cylinder pressure and ROHR at partial load (2400 rpm–10.5 bar BMEP).</p>
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<p>Effect of increasing H<sub>2</sub> energy share on the main combustion characteristics at partial load (2400 rpm–10.5 bar BMEP): (<b>a</b>) peak in-cylinder pressure and PPRR; (<b>b</b>) IMEP* and combustion efficiency; (<b>c</b>) combustion phasing parameters; (<b>d</b>) turbulent combustion duration.</p>
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<p>Effect of increasing H<sub>2</sub> energy share on the main combustion characteristics at partial load (2400 rpm–10.5 bar BMEP): (<b>a</b>) peak in-cylinder pressure and PPRR; (<b>b</b>) IMEP* and combustion efficiency; (<b>c</b>) combustion phasing parameters; (<b>d</b>) turbulent combustion duration.</p>
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<p>Effect of SOI variation on diesel fuel injection law for diesel case at partial load (2400 rpm–10.5 bar BMEP).</p>
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<p>Influence of H2 energy share and diesel fuel SOI on main combustion characteristics at partial load (2400 rpm–10.5 bar BMEP): (<b>a</b>) peak in-cylinder pressure; (<b>b</b>) PPRR; (<b>c</b>) IMEP*; (<b>d</b>) combustion efficiency; (<b>e</b>) CA10-90.</p>
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<p>Comparison among baseline diesel, optimized diesel, and optimized H<sub>2</sub>-D DF cases in terms of the following: (<b>a</b>) peak in-cylinder pressure; (<b>b</b>) IMEP* and combustion efficiency; (<b>c</b>) combustion phasing; (<b>d</b>) combustion duration at partial load (2400 rpm–10.5 bar BMEP).</p>
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<p>Comparison among baseline diesel, optimized diesel, and optimized H<sub>2</sub>-D DF cases in terms of the following: (<b>a</b>) peak in-cylinder pressure; (<b>b</b>) IMEP* and combustion efficiency; (<b>c</b>) combustion phasing; (<b>d</b>) combustion duration at partial load (2400 rpm–10.5 bar BMEP).</p>
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<p>Variation in the equivalence ratio of the H<sub>2</sub>-air premixed charge with increasing H<sub>2</sub> energy share at full load (4000 rpm–13.5 bar BMEP).</p>
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<p>Effect of increasing H<sub>2</sub> energy share on in-cylinder pressure and ROHR at full load (4000 rpm–13.5 bar BMEP).</p>
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<p>Effect of increasing H<sub>2</sub> energy share on main combustion characteristics at full load (4000 rpm–13.5 bar BMEP): (<b>a</b>) peak in-cylinder pressure and PPRR; (<b>b</b>) IMEP* and combustion efficiency; (<b>c</b>) combustion phasing parameters; (<b>d</b>) turbulent combustion duration.</p>
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<p>Effect of increasing H<sub>2</sub> energy share on main combustion characteristics at full load (4000 rpm–13.5 bar BMEP): (<b>a</b>) peak in-cylinder pressure and PPRR; (<b>b</b>) IMEP* and combustion efficiency; (<b>c</b>) combustion phasing parameters; (<b>d</b>) turbulent combustion duration.</p>
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<p>Effect of SOI variation on diesel fuel injection law for diesel case at full load (4000 rpm–13.5 bar BMEP).</p>
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<p>Influence of H<sub>2</sub> energy share and diesel fuel SOI on main combustion characteristics at full load (4000 rpm–13.5 bar BMEP): (<b>a</b>) peak in-cylinder pressure; (<b>b</b>) PPRR; (<b>c</b>) IMEP*; (<b>d</b>) combustion efficiency; (<b>e</b>) CA10-90.</p>
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<p>SOI comparison between baseline and optimized (best) diesel fuel injection strategy for different H<sub>2</sub> energy shares.</p>
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<p>Comparison between the main combustion characteristics before and after the diesel fuel SOI optimization at full load (4000 rpm–13.5 bar BMEP): (<b>a</b>) peak in-cylinder pressure and PPRR; (<b>b</b>) IMEP* and combustion efficiency; (<b>c</b>) combustion phasing parameters; (<b>d</b>) turbulent combustion duration.</p>
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<p>Comparison between baseline diesel operation and optimized 80% H<sub>2</sub>-D DF combustion at full load (4000 rpm–13.5 bar BMEP) in terms of in-cylinder pressure and ROHR.</p>
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22 pages, 1516 KiB  
Article
Unlocking the Potential of Pick-Up Points in Last-Mile Delivery in Relation to Gen Z: Case Studies from Greece and Italy
by Efstathios Bouhouras, Stamatia Ftergioti, Antonio Russo, Socrates Basbas, Tiziana Campisi and Pantelis Symeon
Appl. Sci. 2024, 14(22), 10629; https://doi.org/10.3390/app142210629 - 18 Nov 2024
Viewed by 311
Abstract
Pick-up points (PUPs) have become a very attractive alternative for conventional home deliveries due to the growth of e-commerce. This paper investigates the level of satisfaction of the students (Gen Z) as well as the research, teaching, and administrative staff of the Aristotle [...] Read more.
Pick-up points (PUPs) have become a very attractive alternative for conventional home deliveries due to the growth of e-commerce. This paper investigates the level of satisfaction of the students (Gen Z) as well as the research, teaching, and administrative staff of the Aristotle University of Thessaloniki (AUTH), Greece, and the University of Enna “Kore”, Italy, implemented in November 2023. Optimizing the PUP users’ satisfaction is contingent upon various aspects, including but not limited to location accessibility, expedient pick-up procedures, unambiguous communication, and ensured item availability. The research recorded information about the users’ knowledge about the specific service, their level of satisfaction, their preferences on when and how they use the service, and information about the types of goods they order using the PUPs as their point of collection. The analysis of the collected data revealed very interesting findings that could be useful to the providers of this service, especially when taking into consideration that the majority of the poll’s participants are familiar with the existence of the PUPs in the Municipality of Thessaloniki, that they use this service mainly occasionally, and that the majority are quite pleased with the level of the provided services (accessibility, availability, safety, and security). For the case of Enna in Sicily, similar trends are shown: a high percentage of respondents are familiar with PUPs, and they use pick-up points occasionally and are pleased with the provided level of service. The comparative statistical analysis makes it possible to compare two contexts located in areas of the Mediterranean, i.e., two urban areas with different population sizes but with similar habits on the part of the university student cluster. Full article
(This article belongs to the Special Issue Sustainable Urban Mobility)
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<p>Distribution of collected data regarding Section’s A questions (Greek case study: blue, Italian case study: green).</p>
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<p>Distribution of collected data regarding Section’s A questions (Greek case study: blue, Italian case study: green).</p>
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<p>Distribution of collected data regarding Section’s B questions (Greek case study: blue/red, Italian case study: green/red).</p>
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<p>Distribution of collected data regarding Section’s B questions (Greek case study: blue/red, Italian case study: green/red).</p>
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<p>Distribution of collected data regarding Section’s B questions (Greek case study: blue/red, Italian case study: green/red).</p>
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<p>Distribution of collected data regarding Section’s B questions (Greek case study: blue/red, Italian case study: green/red).</p>
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15 pages, 4350 KiB  
Article
The Effect of Caffeine Exposure on Sleep Patterns in Zebrafish Larvae and Its Underlying Mechanism
by Yuanzheng Wei, Zongyu Miao, Huixin Ye, Meihui Wu, Xinru Wei, Yu Zhang and Lei Cai
Clocks & Sleep 2024, 6(4), 749-763; https://doi.org/10.3390/clockssleep6040048 - 18 Nov 2024
Viewed by 377
Abstract
The effect of caffeine on the behavior and sleep patterns of zebrafish larvae, as well as its underlying mechanisms, has been a topic of great interest. This study aimed to investigate the impact of caffeine on zebrafish larval sleep/wake behavior and the expression [...] Read more.
The effect of caffeine on the behavior and sleep patterns of zebrafish larvae, as well as its underlying mechanisms, has been a topic of great interest. This study aimed to investigate the impact of caffeine on zebrafish larval sleep/wake behavior and the expression of key regulatory genes such as cAMP-response element binding protein (CREB) and adenosine (ADA) in the sleep pathway. To begin, the study determined the optimal dose and duration of caffeine exposure, with the optimal doses found to be 31.25 μM, 62.5 μM, and 120 μM. Similarly, the optimal exposure time was established as no more than 120 h, ensuring a mortality rate of less than 10%. The confirmation of these conditions was achieved through the assessment of angiogenesis and the inflammatory reaction. As a result, the treatment time point of 24 h post-fertilization (hpf) was selected to examine the effects of caffeine on zebrafish larval sleep rhythm (48 h, with a light cycle of 14:10). Furthermore, the study analyzed the expression of clock genes (bmal1a, per1b, per2, per3, cry2), adenosine receptor genes (adora1a, adora1b, adora2aa, adora2ab, adora2b), and key regulatory factors (CREB and ADA). The research confirmed that caffeine could induce sleep pattern disorders, significantly upregulate adenosine receptor genes (adora1a, adora1b, adora2a, adora2ab, adora2b) (p < 0.05), and markedly decrease the total sleep time and sleep efficiency of the larvae. Additionally, the activity of ADA significantly increased during the exposure (p < 0.001), and the tissue-specific expression of CREB was also significantly increased, as assessed by immunofluorescence. Caffeine may regulate circadian clock genes through the ADA/ADORA/CREB pathway. These findings not only enhance our understanding of the effects of caffeine on zebrafish larvae but also provide valuable insights into the potential impact of caffeine on human behavior and sleep. Full article
(This article belongs to the Section Animal Basic Research)
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<p>Percent survival (<b>A</b>) and proportion of normal individuals (<b>B</b>) of developing zebrafish embryos after exposure to caffeine. The red horizontal line indicates the threshold when the proportion is 80%. The gray shading marks the time before sleep rhythms were established. Each treatment group’s (31.25 μM, 62.5 μM, 125 μM, 250 μM, 500 μM, 1000 μM, 2000 μM caffeine) survival was assessed, and the number of normal individuals was counted (3 trials, <span class="html-italic">n</span> = 30).</p>
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<p>Developmental toxicity assessment of zebrafish embryos in response to caffeine exposure. (<b>A</b>) represents the development status of dorsal longitudinal anastomotic vessels (DLAVs) and subintestinal vein (SIV) after 72 h of caffeine exposure; (<b>B</b>,<b>C</b>) represent the NR labeling of macrophages and SB labeling of neutrophils in 72 h post-embryonic (hpe) caffeine-treated zebrafish larvae; (<b>D</b>) represents the DLAV numbers; (<b>E</b>) represents the SIV number; (<b>F</b>) represents the number of macrophages; (<b>G</b>) represents the number of neutrophils. The results are presented as mean ± SD (three trials, <span class="html-italic">n</span> = 10) and a bar chart marked with different letters indicates significant differences (<span class="html-italic">p</span> &lt; 0.05). The letters a, b, and c on the bar chart represent the levels of significance. The same letter indicates no significant difference between the two, while different letters indicate a significant difference (<span class="html-italic">p</span> &lt; 0.05).</p>
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<p>Sleep/wake behavior alterations and relevant parameters from 120 hpf to 168 hpf after caffeine was removed. (<b>A</b>) Rest total and (<b>B</b>) waking activity were averaged every 10 min and then normalized to the control values. The red lines (31.25 μM), green lines (62.5 μM), and purple lines (125 μM) show the averages of the different treated groups with caffeine, and the blue line shows the average of the control group. The black and white bars in the abscissa indicate the dark (10 h) and light (14 h) periods. (<b>C</b>–<b>F</b>) Histograms for rest total, rest bouts length, activity total, and waking activity. Values are means of quadruplicate groups and presented as mean ± standard deviation (SD, <span class="html-italic">n</span> = 24); main effect means that values in the same column with different superscripts are significantly different (<span class="html-italic">p</span> &lt; 0.05). (<b>G</b>) Heatmap of sleep-wake related parameters. Rows indicate different behavioral parameters; columns indicate different groups. The black and white bars represente the dark and light periods respectively. The letters a, b, and c on the bar chart represent the levels of significance. The same letter indicates no significant difference between the two, while different letters indicate a significant difference (<span class="html-italic">p</span> &lt; 0.05).</p>
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<p>Relative mRNA expression of clock genes from 120 hpf to 168 hpf after caffeine was removed. The relative expression of each gene compared to the reference gene β-actin is shown as a bar graph. (<b>A</b>) is the <span class="html-italic">Bmal1a</span> gene, (<b>B</b>) is the <span class="html-italic">Per1b</span> gene, (<b>C</b>) is the <span class="html-italic">Cry1a</span> gene, (<b>D</b>) is the <span class="html-italic">Cry2</span> gene, (<b>E</b>) is the <span class="html-italic">Per2</span> gene, (<b>F</b>) is the <span class="html-italic">Per3</span> gene. The black bars (dark1, dark2) and light gray bars (light1, light2) in the abscissa indicate the two dark/light periods—the dark (10 h) and light (14 h) periods. The results represent the mean ± SD (<span class="html-italic">n</span> = 3), and a bar chart marked with different letters indicates significant differences (<span class="html-italic">p</span> &lt; 0.05). The letters a, b, and c on the bar chart represent the levels of significance. The same letter indicates no significant difference between the two, while different letters indicate a significant difference (<span class="html-italic">p</span> &lt; 0.05).</p>
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<p>Relative mRNA expression of adenosine receptor genes from 120 hpf to 168 hpf after caffeine was removed and ADA enzyme activity in response to different concentrations of caffeine. (<b>A</b>) to (<b>E</b>) represent the diurnal expression patterns of five adenosinergic receptor genes under the influence of different concentrations of caffeine, where (<b>A</b>) is the adora1a gene, (<b>B</b>) is the adora1b gene, (<b>C</b>) is the adora2aa gene, (<b>D</b>) is the adora2ab gene, and (<b>E</b>) is the adora2b gene. (<b>F</b>) represents the enzyme activity of ADA under the influence of different concentrations of caffeine. Both the adenosinergic receptor genes and the ADA enzyme activity show an upward trend. The letters a, b, and c on the bar chart represent the levels of significance. The same letter indicates no significant difference between the two, while different letters indicate a significant difference (<span class="html-italic">p</span> &lt; 0.05).</p>
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<p>Immunofluorescence analysis of CREB in zebrafish brain tissue following exposure to varying concentrations of caffeine. (<b>A</b>) is designated as the control cohort, while (<b>B</b>–<b>D</b>) correspond to the experimental groups treated with caffeine at concentrations of 31.25 μM, 62.5 μM, and 125 μM, respectively. The term “Nucleus” refers to the cellular nuclear staining visualized in blue, “CREB” denotes the immunofluorescent labeling of the CREB protein in green, and “Merge” illustrates the composite image where the blue and green channels are superimposed.</p>
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20 pages, 3061 KiB  
Article
Concentrating Cocoa Polyphenols—Clarification of an Aqueous Cocoa Extract by Protein Precipitation and Filtration
by Nicole Beeler, Tilo Hühn, Sascha Rohn and Renato Colombi
Membranes 2024, 14(11), 242; https://doi.org/10.3390/membranes14110242 - 17 Nov 2024
Viewed by 623
Abstract
The seeds of Theobroma cacao L. are rich in antioxidant flavonoids such as flavan-3-ols, which are valued for their health benefits. In this context, it is of interest to improve flavanol content in cocoa extracts. The present study aimed at improving the clarification [...] Read more.
The seeds of Theobroma cacao L. are rich in antioxidant flavonoids such as flavan-3-ols, which are valued for their health benefits. In this context, it is of interest to improve flavanol content in cocoa extracts. The present study aimed at improving the clarification process of an aqueous cocoa extract using protein precipitation and filtration. Five pH modifications and two bentonite amounts were tested for their effects on protein precipitation and flavanol content. Micro- and ultrafiltration as a subsequent step was done by testing three different ceramic membranes (30, 80, and 200 nm). Lower pH in pre-treatment reduced protein content and kept flavanols constant, while at higher pH, flavanols were reduced up to 40%. Larger membrane pores enhanced polyphenol permeation, while smaller pores limited protein permeation. Adjusting pH to the isoelectric point increased protein adsorption, improving filtration quality despite decreased permeate flux. However, membrane fouling results in higher permeate quality due to increased selectivity. Furthermore, the addition of bentonite during filtration reduced both protein and flavanol content in the permeate, similar to the effects seen in the pre-treatment of the supernatant. Optimizing pH and membrane pore size enhances the recovery and quality of polyphenols during filtration, balancing protein removal and flavanol retention. Full article
(This article belongs to the Section Membrane Applications for Other Areas)
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<p>Schematic diagram of the clarifying process of the cocoa extract with the extraction, pre-treatment, and filtration step.</p>
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<p>Illustration of the CRDM and the experimental set-up with the dynamic crossflow filtration pilot plant. Feed: Supernatant from pre-treatments; Retentate: retained product; Permeate: clarified product. Created in BioRender [<a href="#B38-membranes-14-00242" class="html-bibr">38</a>].</p>
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<p>Yield after pre-treatment of cocoa extracts in terms of solids expressed as dry matter content (<b>a</b>), flavanols (<b>b</b>), and protein contents (<b>c</b>) in the supernatant. And the yield after pre-treatment experiments with and without bentonite addition (bento.add) in terms of solids (<b>d</b>), flavanols (<b>e</b>), and proteins (<b>f</b>) in the supernatant. If marked with (*), no data is available.</p>
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<p>Dry matter (<b>a</b>), flavanol (<b>b</b>), and protein content (<b>c</b>) in g/100 g dry matter (dm) of the cocoa extract and supernatant after pre-treatment. And the content of the cocoa extract and supernatant after pre-treatment experiments with and without bentonite addition (bento.add) in terms of solids (<b>d</b>), flavanols (<b>e</b>), and proteins (<b>f</b>). If marked with (*), no data is available.</p>
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<p>Permeation coefficient of cocoa solids expressed as dry matter content, flavanol, and protein content of the cocoa extract after pre-treatment and filtration with the origin from Cuba. If marked with (*), no data is available.</p>
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<p>Permeate flux (<math display="inline"><semantics> <mrow> <msub> <mrow> <mi>J</mi> </mrow> <mrow> <mi>P</mi> </mrow> </msub> </mrow> </semantics></math>) in kg/(m<sup>2</sup>h) as a function of the WRF at three different membrane pore sizes. Operation conditions: TMP 0.8 bar; Q<sub>f</sub>, 100 L/min; T, 10 °C.</p>
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<p>Permeation coefficient of cocoa solids expressed as dry matter content (<b>a</b>), flavanols (<b>b</b>), and proteins (<b>c</b>) after pH-modification and filtration. As well as permeation of the cocoa solids (<b>d</b>), flavanols (<b>e</b>), and proteins (<b>f</b>) after pH modification with and without bentonite addition (bento.add) and filtration. If marked with (*), no data is available.</p>
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<p>Dry matter (<b>a</b>), flavanol (<b>b</b>), and protein content (<b>c</b>) in g/100 g dry matter (dm) of the cocoa extract and permeate after pre-treatment and filtration. And the content of the cocoa solids (<b>d</b>), flavanols (<b>e</b>), and proteins (<b>f</b>) of the cocoa extract and permeate after pH-modification with and without bentonite addition (bento.add) and filtration. If marked with (*), no data is available.</p>
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<p>Permeate flux (<math display="inline"><semantics> <mrow> <msub> <mrow> <mi>J</mi> </mrow> <mrow> <mi>P</mi> </mrow> </msub> </mrow> </semantics></math>) in kg/(m<sup>2</sup>h) as a function of the WRF at five different pH values for (<b>a</b>) the origin DOM, (<b>b</b>) the origin CUBA, and (<b>c</b>) the origin PERU. Operation conditions: TMP 0.8 bar; Q<sub>f</sub>, 100 L/min; T, 10 °C.</p>
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33 pages, 394 KiB  
Article
On the Problem of the Uniqueness of Fixed Points and Solutions for Quadratic Fractional-Integral Equations on Banach Algebras
by Kinga Cichoń, Mieczysław Cichoń and Maciej Ciesielski
Symmetry 2024, 16(11), 1535; https://doi.org/10.3390/sym16111535 - 16 Nov 2024
Viewed by 328
Abstract
In this paper, we study the problem of the uniqueness of fixed points for operators defined on subspaces of the space of continuous functions C[a,b] equipped with norms stronger than the supremum norm. We are particularly interested in [...] Read more.
In this paper, we study the problem of the uniqueness of fixed points for operators defined on subspaces of the space of continuous functions C[a,b] equipped with norms stronger than the supremum norm. We are particularly interested in Hölder spaces since they are natural ranges of integral operators of fractional order. Our goal is to preserve the expected regularity of the fixed points (solutions of the equations) when investigating their uniqueness, without assuming a contraction condition on the space under study. We claim some symmetry between the case of the obtained results and the case of the classical Banach fixed-point theorem in such spaces, even for operators which are not necessarily contractions in the sense of the norm of these subspaces. This result is of particular interest for the study of quadratic integral equations, and as an application example we prove the uniqueness theorem for such a kind equations with general fractional order integral operators, which are not necessarily contractions, in a suitably constructed generalized Hölder space. Full article
(This article belongs to the Special Issue New Trends in Fixed Point Theory with Emphasis on Symmetry)
12 pages, 675 KiB  
Article
Interpretable Embeddings for Next Point-of-Interest Recommendation via Large Language Model Question–Answering
by Jiubing Chen, Haoyu Wang, Jianxin Shang and Chaomurilige
Mathematics 2024, 12(22), 3592; https://doi.org/10.3390/math12223592 - 16 Nov 2024
Viewed by 348
Abstract
Next point-of-interest (POI) recommendation provides users with location suggestions that they may be interested in, allowing them to explore their surroundings. Existing sequence-based or graph-based POI recommendation methods have matured in capturing spatiotemporal information; however, POI recommendation methods based on large language models [...] Read more.
Next point-of-interest (POI) recommendation provides users with location suggestions that they may be interested in, allowing them to explore their surroundings. Existing sequence-based or graph-based POI recommendation methods have matured in capturing spatiotemporal information; however, POI recommendation methods based on large language models (LLMs) focus more on capturing sequential transition relationships. This raises an unexplored challenge: how to leverage LLMs to better capture geographic contextual information. To address this, we propose interpretable embeddings for next point-of-interest recommendation via large language model question–answering, named QA-POI, which transforms the POI recommendation task into obtaining interpretable embeddings via LLM prompts, followed by lightweight MLP fine-tuning. We introduce question–answer embeddings, which are generated by asking LLMs yes/no questions about the user’s trajectory sequence. By asking spatiotemporal questions about the trajectory sequence, we aim to extract as much spatiotemporal information from the LLM as possible. During training, QA-POI iteratively selects the most valuable subset of potential questions from a set of questions to prompt the LLM for the next POI recommendation. It is then fine-tuned for the next POI recommendation task using a lightweight Multi-Layer Perceptron (MLP). Extensive experiments on two datasets demonstrate the effectiveness of our approach. Full article
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<p>An example of a check-in sequence in a trajectory where the selection of candidate POIs is influenced by multiple factors, with geographical factors playing a significant role. Note that the dashed arrows denote the historical trajectory while the dotted ones stand for the potential visit of the next check-in. The numbers denote the order of check-ins.</p>
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<p>It illustrates the architecture of our proposed model. It consists of (1) a prompt template for generating input text modules, (2) a module for learning to answer yes/no question sets, (3) an MLP fine-tuning module, and (4) a prediction module. In particular, (1) is our proposed prompt template that incorporates various comprehensive factors to prompt the LLM to automatically generate questions; (2) continues to input these questions into the LLM to produce representation vectors and trims the question set based on the prediction results; (3) uses an MLP to further fine-tune the model parameters; and (4) the prediction module outputs a top-k set of potential target candidates.</p>
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<p>The performance comparison about the dimension <span class="html-italic">d</span>, the number of question <span class="html-italic">P</span>. The circles and squares denote the scores on Foursquare and Gowalla, respectively.</p>
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26 pages, 5734 KiB  
Article
Big Data Analysis of ‘VTuber’ Perceptions in South Korea: Insights for the Virtual YouTuber Industry
by Hyemin Kim and Jungho Suh
Journal. Media 2024, 5(4), 1723-1748; https://doi.org/10.3390/journalmedia5040105 - 15 Nov 2024
Viewed by 506
Abstract
The global VTuber market is experiencing rapid growth, with VTubers extending beyond mere content creators to be utilized in various fields such as social interaction, public relations, and health. VTubers have the potential to expand the existing content market and contribute to increasing [...] Read more.
The global VTuber market is experiencing rapid growth, with VTubers extending beyond mere content creators to be utilized in various fields such as social interaction, public relations, and health. VTubers have the potential to expand the existing content market and contribute to increasing economic and public value. This study aims to investigate the perception of VTubers in South Korea and to provide insights that can contribute to the global activation of the VTuber entertainment industry. For this purpose, unstructured data on VTubers from the past three years, during which interest in VTubers has significantly grown in South Korea, was collected. A total of 57,891 samples were gathered from Naver, Daum, and Google, of which 50 highly relevant data points between VTubers and users were selected for analysis. First, key terms such as ‘Broadcast’, ‘YouTube’, ‘Live’, ‘Game’, ‘Youtuber’, ‘Japan’, ‘Character’, ‘Video’, ‘Sing’, ‘Virtual’, ‘Woowakgood’, ‘Fan’, ‘Idol’, ‘Korea’, ‘Twitch’, ‘IsegyeIdol’, ‘Communication’, ‘Worldview’, ‘VTuberIndustry’, ‘Contents’, ‘AfricaTV’, ‘Nijisanji’, and ‘Streamer’ were extracted. Second, CONCOR analysis revealed four clusters: ‘Famous VTubers’, ‘Features of VTubers’, ‘VTuber Industry’, and ‘VTuber Platforms’. Based on these findings, the study offers various academic and practical implications regarding VTubers in South Korea and explores the potential for global growth in the VTuber industry. Full article
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<p>Trend graph of ‘VTuber’ mentions on Google Trends.</p>
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<p>Time series analysis of data collection for ‘VTubers’.</p>
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<p>Centrality analysis and ego network density analysis for ‘VTuber’.</p>
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<p>CONCOR analysis network of 50 nodes for ‘VTuber’.</p>
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<p>The four groups and clusters among the groups.</p>
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19 pages, 3906 KiB  
Article
Comparison of Different 3D Surface Registration-Based Methods to Assess Facial Asymmetry
by Annalisa Cappella, Riccardo Solazzo, Luisa Gigante, Alice Gervasoni, Daniele Maria Gibelli, Claudia Dolci, Gianluca Martino Tartaglia and Chiarella Sforza
Diagnostics 2024, 14(22), 2573; https://doi.org/10.3390/diagnostics14222573 - 15 Nov 2024
Viewed by 278
Abstract
Background/Objectives: Facial asymmetry is gaining an increasing diagnostic interest in many clinical contexts. Several three-dimensional surface-based methods have been proposed for its assessment; however, they might provide non-equivalent data. Since there is a lack of comparative studies in these terms, this study aims [...] Read more.
Background/Objectives: Facial asymmetry is gaining an increasing diagnostic interest in many clinical contexts. Several three-dimensional surface-based methods have been proposed for its assessment; however, they might provide non-equivalent data. Since there is a lack of comparative studies in these terms, this study aims to compare three methods for assessing the asymmetry of the face and facial thirds, thus addressing whether the potential differences can be considered clinically acceptable or not. Methods: Two ‘maxillofacial’ methods based on the trigeminal nerve distribution and one ‘orthodontic’ method based on reference horizontal planes were used to identify the facial thirds on 3D facial models of 80 Italian healthy adults to calculate the asymmetry of the face, and the upper, middle, and lower thirds of the face differently selected by each method. As a measure of asymmetry, the Root Mean Square value was calculated through a mirroring surface-based registration. Intra- and inter-operator reliability was verified for each method. Differences and interchangeability between the methods were tested, respectively, by two-way repeated measures ANOVA (Analysis of Variance) and Bland–Altman and Similarity Percentage model analysis. Additionally, the time required to perform each method was assessed. Results: All methods demonstrated excellent intra- and inter-operator reliability. While the ANOVA analysis found significant differences (p < 0.001) for the majority of facial Regions of Interest between each method, the Bland–Altman analysis revealed that the differences were clinically acceptable (<0.50 mm) for all facial regions between the trigeminal methods, and for the face and the upper third of the face between the orthodontic method, which was revealed to be faster, and the trigeminal ones. The additional similarity percentage model provided visual support for the complete interchangeability of the two trigeminal methods, as evidenced by the lower Coefficient of Variation value. Conclusions: There is no best method for assessing facial asymmetry that applies to all types of clinical settings, as we have shown that different methods may not be completely interchangeable. However, we suggest that the methods based on the trigeminal subdivision can be used interchangeably in contexts where the morpho-functional analysis of maxillofacial regions with different embryological origins is considered. Thus, the clinical setting imposes the choice of one method over another and, as we have pointed out, the consequent comparison of data with those obtained with methods whose interchangeability has been demonstrated. Full article
(This article belongs to the Special Issue Diagnostics and Management in Oral and Maxillofacial Medicine)
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<p>Selection of the facial area of interest. (<b>a</b>) 3D model of the participant; (<b>b</b>) selection of the FAI through perimetral landmarks; (<b>c</b>) FAI.</p>
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<p>Method 1 for ROI selection. (<b>a</b>) FAI; (<b>b</b>) hemiface (green); (<b>c</b>) thirds of the face: upper third (orange), middle third (pink), lower third (light blue) of the face.</p>
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<p>Method 2 for ROI selection. (<b>a</b>) FAI; (<b>b</b>) face (green); (<b>c</b>) facial thirds: upper third (orange), middle third (pink), lower third (light blue) of the face.</p>
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<p>Method 3 for ROI selection. (<b>a</b>) FAI; (<b>b</b>) face (green); (<b>c</b>) facial thirds: upper third (orange), middle third (pink), lower third (light blue) of the face.</p>
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<p>Bland–Altman plots for the overall RMS obtained by the three methods and pairs compared (total data including hemifaces and facial thirds). The black dots represent the differences of paired measurements plotted against their mean values. The continuous blue line indicates the mean difference (bias); superior and lower dashed lines represent the 95% interval of agreement (respectively, upper Limit of Agreement (uLoA) and lower Limit of Agreement (lLoA)). The black continuous line represents the 0. The green area represents the limits of agreement from −1.96<span class="html-italic">s</span> to +1.96<span class="html-italic">s</span> (<span class="html-italic">s</span>: standard deviation of the differences). The light blue areas delimited by dotted blue lines represents the 95% confidence interval of the limits of agreement and bias. The clinical acceptability is set to 0.50 mm, and, whenever the amplitude of the LoA is greater than this value, the methods cannot be considered clinically interchangeable. The plots where Method 1 is compared with Method 2 show clinical acceptability. M: Method.</p>
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<p>Bland–Altman plots for the RMS of the hemiface obtained by the three methods and pair compared (M2 and M3 are identical). The black dots represent the differences of paired measurements plotted against their mean values. The continuous blue line indicates the mean difference (bias); superior and lower dashed lines represent the 95% interval of agreement (respectively, upper Limit of Agreement (uLoA) and lower Limit of Agreement (lLoA)). The black continuous line represents the 0. The green area represents the limits of agreement from −1.96<span class="html-italic">s</span> to +1.96<span class="html-italic">s</span> (<span class="html-italic">s</span>: standard deviation of the differences). The light blue areas delimited by dotted blue lines represents the 95% confidence interval of the limits of agreement and bias. The clinical acceptability is set to 0.50 mm, and, whenever the amplitude of the LoA is greater than this value, the methods cannot be considered clinically interchangeable. The plots where the hemiface of Method 1 is compared with the face of Methods 2 and 3. M: Method.</p>
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<p>Bland–Altman plots for the RMS of the facial thirds (upper, middle, and lower) were obtained with the three methods and pair compared. The black dots represent the differences of paired measurements plotted against their mean values. The continuous blue line indicates the mean difference (bias); superior and lower dashed lines represent the 95% interval of agreement (respectively, upper Limit of Agreement (uLoA) and lower Limit of Agreement (lLoA)). The black continuous line represents the 0. The green area represents the limits of agreement from −1.96<span class="html-italic">s</span> to +1.96<span class="html-italic">s</span> (<span class="html-italic">s</span>: standard deviation of the differences). The light blue areas delimited by dotted blue lines represents the 95% confidence interval of the limits of agreement and bias. The clinical acceptability is set to 0.50 mm, and, whenever the amplitude of the LoA is greater than this value, the methods are not considered clinically interchangeable. The plots where Method 1 is compared with Method 2 show clinical acceptability for all thirds of the face, with the amplitude &lt; 0.5 mm. Only the plots of the upper third of the face showed clinical acceptability when Method 3 is compared with Method 1 and Method 2. M: Method.</p>
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<p>The percentage similarity histogram of each pair method comparison (method 2 and 3 versus method 1 and method 3 versus method 2). The horizontal axis represents the percentage similarity values in intervals, and the vertical axis represents the number (percentage of the total number) of percentage similarity values in each interval.</p>
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