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Search Results (463)

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13 pages, 3239 KiB  
Article
Morphological and Performance Biomechanics Profiles of Draft Preparation American-Style Football Players
by Monique Mokha, Maria Berrocales, Aidan Rohman, Andrew Schafer, Jack Stensland, Joseph Petruzzelli, Ahmad Nasri, Talia Thompson, Easa Taha and Pete Bommarito
Biomechanics 2024, 4(4), 685-697; https://doi.org/10.3390/biomechanics4040049 - 10 Nov 2024
Viewed by 357
Abstract
Background/Objectives: Using advanced methodologies may enhance athlete profiling. This study profiled morphological and laboratory-derived performance biomechanics by position of American-style football players training for the draft. Methods: Fifty-five players were categorized into three groups: Big (e.g., lineman; n = 17), Big–skill (e.g., tight [...] Read more.
Background/Objectives: Using advanced methodologies may enhance athlete profiling. This study profiled morphological and laboratory-derived performance biomechanics by position of American-style football players training for the draft. Methods: Fifty-five players were categorized into three groups: Big (e.g., lineman; n = 17), Big–skill (e.g., tight end; n = 11), and Skill (e.g., receiver; n = 27). Body fat (BF%), lean body mass (LBM), and total body mass were measured using a bioelectrical impedance device. Running ground reaction force (GRF) and ground contact time (GCT) were obtained using an instrumented treadmill synchronized with a motion capture system. Dual uniaxial force plates captured countermovement jump height (CMJ-JH), normalized peak power (CMJ-NPP), and reactive strength. Asymmetry was calculated for running force, GCT, and CMJ eccentric and concentric impulse (IMP). MANOVA determined between-group differences, and radar plots for morphological and performance characteristics were created using Z-scores. Results: There was a between-group difference (F(26,80) = 5.70, p < 0.001; Wilk’s Λ = 0.123, partial η2 = 0.649). Fisher’s least squares difference post hoc analyses showed that participants in the Skill group had greater JH, CMJ-NPP, reactive strength, and running GRF values versus Big players but not Big–skill players (p < 0.05). Big athletes had greater BF%, LBM, total body mass, and GCT values than Skill and Big–skill athletes (p < 0.05). Big–skill players had greater GCT asymmetry than Skill and Big players (p < 0.05). Asymmetries in running forces, CMJ eccentric, and concentric IMP were not different (p > 0.05). Morphological and performance biomechanics differences are pronounced between Skill and Big players. Big–skill players possess characteristics from both groups. Laboratory-derived metrics offer precise values of running and jumping force strategies and body composition that can aid sports science researchers and practitioners in refining draft trainee profiles. Full article
(This article belongs to the Special Issue Biomechanics in Sport, Exercise and Performance)
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<p>Protocol schematic.</p>
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<p>Morphological measurements taken using InBody 270.</p>
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<p>Running assessment using motion capture and instrumented treadmill.</p>
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<p>Countermovement jump assessment using dual uniaxial force plates.</p>
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<p>Radar plot of the 12 morphological and biomechanics performance characteristics based on player position group. CMJ = countermovement jump.</p>
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11 pages, 1668 KiB  
Article
Development of Traffic Scheduling Based on TSN in Smart Substation Devices
by Xin Mei, Jin Wang, Chang Liu, Chang Liu, Jiangpei Xu, Zishang Cui, Lijun Peng and Bing Chen
Appl. Sci. 2024, 14(22), 10135; https://doi.org/10.3390/app142210135 - 5 Nov 2024
Viewed by 497
Abstract
Smart substations are an important trend in substation construction. With increasing data traffic, it is difficult for the traditional Ethernet network to meet the real-time requirements of control information in smart substations. Hence, in this paper, a deterministic network architecture for substations based [...] Read more.
Smart substations are an important trend in substation construction. With increasing data traffic, it is difficult for the traditional Ethernet network to meet the real-time requirements of control information in smart substations. Hence, in this paper, a deterministic network architecture for substations based on time-sensitive networks (TSN) has been developed in order to realize the domain-wide time synchronization and efficient real-time communication of the “three-layer and two-network” model in smart substations. Furthermore, a design scheme for substation automation equipment based on TSN is proposed. The proposed device realizes the timely transmission of real-time control information packets by utilizing the Earliest TxTime First (ETF) Qdisc technology of Linux and the timing sending capability of Intel 210 NIC. Furthermore, it collaborates with the time-aware shaper (TAS) traffic scheduling mechanism of TSN switches to ensure the end-to-end deterministic delay of time-sensitive traffic. As a result, it provides efficient real-time communication services with low latency and jitter for smart substation automation systems. Full article
(This article belongs to the Special Issue AI-Based Methods for Object Detection and Path Planning)
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<p>The three-layer two-network model.</p>
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<p>TAS gating mechanism.</p>
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<p>Combined hardware and software egress scheduling scheme.</p>
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<p>ETF soft scheduling in Linux.</p>
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<p>Test of timing and sending function of TSN end device.</p>
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<p>Latency and jitter without TSN.</p>
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<p>Latency and jitter with TSN.</p>
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19 pages, 3584 KiB  
Article
High-Efficiency e-Powertrain Topology by Integrating Open-End Winding and Winding Changeover for Improving Fuel Economy of Electric Vehicles
by Kyoung-Soo Cha, Jae-Hyun Kim, Sung-Woo Hwang, Myung-Seop Lim and Soo-Hwan Park
Mathematics 2024, 12(21), 3415; https://doi.org/10.3390/math12213415 - 31 Oct 2024
Viewed by 482
Abstract
The fuel economy of electric vehicles (EVs) is an important factor in determining the competitiveness of EVs. Since the fuel economy is affected by the efficiency of an e-powertrain composed of a motor and inverter, it is necessary to select a high-efficiency topology [...] Read more.
The fuel economy of electric vehicles (EVs) is an important factor in determining the competitiveness of EVs. Since the fuel economy is affected by the efficiency of an e-powertrain composed of a motor and inverter, it is necessary to select a high-efficiency topology for the e-powertrain. In this paper, a novel topology of e-powertrains to improve the fuel economy of EVs is proposed. The proposed topology aims to improve the system efficiency by integrating open-end winding (OEW) and winding changeover (WC). The proposed OEW-PMSM with WC enables to drive a permanent magnet synchronous motor (PMSM) in four different modes. Each mode can increase inverter efficiency and motor efficiency by changing motor parameters and maximum modulation index. In this paper, the system efficiency of the proposed topology was evaluated using electromagnetic finite element analysis and a loss model of power semiconductors. In addition, the vehicle simulations were performed to evaluate the fuel economy of the proposed topology, thereby proving the superiority of the proposed topology compared with the conventional PMSM. Full article
(This article belongs to the Section Engineering Mathematics)
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<p>Schematic of (<b>a</b>) dual inverter fed OEW-PMSM and (<b>b</b>) hexagonal modulation diagram.</p>
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<p>Comparison of current vector for flux-weakening control between conventional three-phase PMSMs and OEW-PMSMs.</p>
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<p>Comparison of torque-speed curve between OEW-PMSM and conventional PMSM for maintaining power density.</p>
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<p>Characteristics and switching loss of power semiconductors: (<b>a</b>) Characteristics of IGBT, (<b>b</b>) diode and switching loss of (<b>c</b>) IGBT, (<b>d</b>) diode.</p>
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<p>Power factor between armature current and reference voltage from each inverter.</p>
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<p>Principle of the winding changeover. (<b>a</b>) Example circuit for winding changeover and (<b>b</b>) current flow in series and parallel mode.</p>
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<p>Electromagnetic characteristics of conventional PMSM with (<b>a</b>) series mode and (<b>b</b>) parallel mode.</p>
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<p>Schematic of proposed OEW-PMSM with WC.</p>
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<p>Operation range of proposed topology.</p>
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<p>Algorithm for selecting operation mode to achieve high system efficiency drive.</p>
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<p>Motor efficiency map for (<b>a</b>) three-phase parallel mode, (<b>b</b>) OEW parallel mode, (<b>c</b>) three-phase series mode, and (<b>d</b>) OEW series mode.</p>
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<p>Inverter efficiency map for (<b>a</b>) three-phase parallel mode, (<b>b</b>) OEW parallel mode, (<b>c</b>) three-phase series mode, and (<b>d</b>) OEW series mode.</p>
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<p>System efficiency map for (<b>a</b>) three-phase parallel mode, (<b>b</b>) OEW parallel mode, (<b>c</b>) three-phase series mode, and (<b>d</b>) OEW series mode.</p>
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<p>Operation mode of proposed topology to maximize the system efficiency.</p>
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<p>Integrated system efficiency map of proposed topology.</p>
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<p>Difference of system efficiency map between conventional PMSM and proposed topology.</p>
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<p>Target driving cycle that is suitable for personal mobility: (<b>a</b>) waveform of vehicle speed and (<b>b</b>) vehicle speed distribution of NYCC cycle.</p>
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<p>Target driving cycle that is suitable for personal mobility.</p>
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<p>Operation points of traction motor and TN-curve for (<b>a</b>) conventional PMSM and (<b>b</b>) proposed topology.</p>
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<p>Comparison of energy consumption for driving cycle according to the e-powertrain topology: (<b>a</b>) rotational speed of the motor, (<b>b</b>) controlled torque of motor, (<b>c</b>) comparison of system loss for each topology, and (<b>d</b>) comparison of consumed energy for each topology.</p>
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<p>Comparison of total consumed energy and fuel economy according to the e-powertrain topology: (<b>a</b>) Comparison of total consumed energy and (<b>b</b>) fuel economy.</p>
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22 pages, 3745 KiB  
Article
Optimal Configuration Model for Large Capacity Synchronous Condenser Considering Transient Voltage Stability in Multiple UHV DC Receiving End Grids
by Lang Zhao, Zhidong Wang, Hao Sheng, Yizheng Li, Xueying Wang, Yao Wang and Haifeng Yu
Energies 2024, 17(21), 5346; https://doi.org/10.3390/en17215346 - 27 Oct 2024
Viewed by 581
Abstract
In a multi-fed DC environment, the UHV DC recipient grid faces significant challenges related to DC phase shift failure and voltage instability due to the high AC/DC coupling strength and low system inertia level. While the new large-capacity synchronous condensers (SCs) can provide [...] Read more.
In a multi-fed DC environment, the UHV DC recipient grid faces significant challenges related to DC phase shift failure and voltage instability due to the high AC/DC coupling strength and low system inertia level. While the new large-capacity synchronous condensers (SCs) can provide effective transient reactive power support, the associated investment and operation costs are high. Therefore, it is valuable to investigate the optimization of SC configuration at key nodes in the recipient grid in a scientific and rational manner. This study begins by qualitatively and quantitatively analyzing the dynamic characteristics of DC reactive power and induction motors under AC faults. The sub-transient and transient reactive power output model is established to describe the SC output characteristics, elucidating the coupling relationship between the SC’s reactive power output and the DC reactive power demand at different time scales. Subsequently, a critical stabilized voltage index for dynamic loads is defined, and the SC’s reactive power compensation target is quantitatively calculated across different time scales, revealing the impact of transient changes in DC reactive power on the transient voltage stability of the multi-fed DC environment with dynamic load integration. Finally, an optimal configuration model for the large-capacity SC is proposed under the critical stability constraint of dynamic loads to maximize the SC’s reactive power support capability at the lowest economic cost. The proposed model is validated in a multi-fed DC area, demonstrating that the optimal configuration scheme effectively addresses issues related to DC phase shift failures and voltage instability resulting from AC bus voltage drops. Full article
(This article belongs to the Section F1: Electrical Power System)
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<p>Comparison of DC current and reactive power consumption. (<b>a</b>) Comparison of DC currents under AC side grounding fault. (<b>b</b>) Comparison of reactive power consumption under AC side grounding fault.</p>
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<p>Comparison of DC current and reactive power consumption. (<b>a</b>) Comparison of DC currents under AC side grounding fault. (<b>b</b>) Comparison of reactive power consumption under AC side grounding fault.</p>
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<p>Reactive power exchange in AC/DC hybrid systems.</p>
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<p>UHV AC/DC hybrid grid topology considering dynamic loads.</p>
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<p>Equivalent circuits for ultra-high voltage AC/DC hybrid grids.</p>
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<p>Power-slip waveform under different conditions.</p>
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<p>Solving process of the optimal configuration model.</p>
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<p>Wiring diagram of a multi-feed DC area in China.</p>
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<p>Sub-function coupling relationship.</p>
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<p>Comparison of ZP and TZ1 transient voltage waveform under AC fault. (<b>a</b>) ZP node voltage at fault A. (<b>b</b>) TZ1 node voltage at fault A. (<b>c</b>) ZP node voltage at fault B. (<b>d</b>) TZ1 node voltage at fault B.</p>
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<p>Comparison of ZP and TZ1 transient voltage waveform under AC fault. (<b>a</b>) ZP node voltage at fault A. (<b>b</b>) TZ1 node voltage at fault A. (<b>c</b>) ZP node voltage at fault B. (<b>d</b>) TZ1 node voltage at fault B.</p>
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<p>DC system reactive power output and demand, dynamic load slip variations. (<b>a</b>) Reactive power output and demand with different numbers of SC accessed. (<b>b</b>) Dynamic load slip variations with different numbers of SC accessed.</p>
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28 pages, 8087 KiB  
Article
Hazard Identification and Risk Assessment During Simultaneous Operations in Industrial Plant Maintenance Based on Job Safety Analysis
by Sung-Jin Kwon, So-Won Choi and Eul-Bum Lee
Sustainability 2024, 16(21), 9277; https://doi.org/10.3390/su16219277 - 25 Oct 2024
Viewed by 944
Abstract
The risk of accidents during simultaneous operations (SIMOPS) in plant maintenance has been increasing. However, research on methods to prevent such accidents has been limited. This study aims to develop a novel framework, hazard identification and risk assessment of simultaneous operations (HIRAS), for [...] Read more.
The risk of accidents during simultaneous operations (SIMOPS) in plant maintenance has been increasing. However, research on methods to prevent such accidents has been limited. This study aims to develop a novel framework, hazard identification and risk assessment of simultaneous operations (HIRAS), for identifying and evaluating potential hazards during concurrent tasks. The framework developed herein is expected to be an effective safety management tool that can help prevent accidents during these operations. To this end, the job location and hazard information in job safety analysis (JSA) were standardized into four attributes. The standardized information was then synchronized spatially and temporally to develop a HIRAS model that identifies and assesses the impact of hazards between operations. The model was tested using 40 JSA documents corresponding to maintenance operations at Company P, a South Korean steel-making company. The model was tested in two scenarios: one with planned operations and the other with unplanned operations in addition to planned operations. The performance evaluation results of the first scenario showed an F1-score of 98.33%. In this case, a recall of 97.52% means that the model identified 97.52% of the hazard-inducing factors. The second scenario was compared with the results of a review by six subject matter experts (SMEs). The comparison of the results identified by the SMEs and the model showed an accuracy of 89.3%. This study demonstrates the potential of JSA, which incorporates the domain knowledge of workers and can be used not only for individual tasks but also as a safety management tool for surrounding operations. Furthermore, by improving the plant maintenance work environment, it is expected to prevent accidents, protect workers’ lives and health, and contribute to the long-term sustainable management of companies. Full article
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<p>Occupational fatalities and the proportion of occupational fatalities due to SIMOPS during 2016–2022.</p>
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<p>General steps of JSA.</p>
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<p>Overall research process.</p>
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<p>Schematic of the R-JSA synchronization model and JSA.</p>
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<p>Connecting the components between SIMOPS and the R-JSA synchronization model. (S) *: Structured data, (U) **: unstructured data.</p>
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<p>The system architecture of HIRAS.</p>
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<p>Example of a relation-oriented JSA form. <sup>1</sup> TR: Task range; <sup>2</sup> S: selection; <sup>3</sup> IOSO: impact on surrounding operations; <sup>4</sup> HR: hazard range; <sup>5</sup> D: direction; <sup>6</sup> R: residue; <sup>7</sup> S: severity; <sup>8</sup> P: probability; <sup>9</sup> RR: risk rating; <sup>10</sup> HV-: the downward direction of the horizontal and vertical; <sup>11</sup> H: horizontal.</p>
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<p>GPT prompt for disaster type classification.</p>
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<p>Criteria and process for selecting items with potential for SIMOPS accidents among disaster types.</p>
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<p>Schematic of data generation for R-JSA synchronization.</p>
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<p>Schematic of exploratory analysis for target–source job identification.</p>
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<p>Schematic of hierarchical analysis for identifying source job hazards.</p>
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<p>Results of the analysis for Scenario 1: (<b>a</b>) first day; (<b>b</b>) second day.</p>
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<p>Results of the analysis for Scenario 1: (<b>a</b>) first day; (<b>b</b>) second day.</p>
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30 pages, 16711 KiB  
Article
Dinochromosome Heterotermini with Telosomal Anchorages
by Alvin Chun Man Kwok, Kosmo Ting Hin Yan, Shaoping Wen, Shiyong Sun, Chongping Li and Joseph Tin Yum Wong
Int. J. Mol. Sci. 2024, 25(20), 11312; https://doi.org/10.3390/ijms252011312 - 21 Oct 2024
Viewed by 571
Abstract
Dinoflagellate birefringent chromosomes (BfCs) contain some of the largest known genomes, yet they lack typical nucleosomal micrococcal-nuclease protection patterns despite containing variant core histones. One BfC end interacts with extranuclear mitotic microtubules at the nuclear envelope (NE), which remains intact throughout the cell [...] Read more.
Dinoflagellate birefringent chromosomes (BfCs) contain some of the largest known genomes, yet they lack typical nucleosomal micrococcal-nuclease protection patterns despite containing variant core histones. One BfC end interacts with extranuclear mitotic microtubules at the nuclear envelope (NE), which remains intact throughout the cell cycle. Ultrastructural studies, polarized light and fluorescence microscopy, and micrococcal nuclease-resistant profiles (MNRPs) revealed that NE-associated chromosome ends persisted post-mitosis. Histone H3K9me3 inhibition caused S-G2 delay in synchronous cells, without any effects at G1. Differential labeling and nuclear envelope swelling upon decompaction indicate an extension of the inner compartment into telosomal anchorages (TAs). Additionally, limited effects of low-concentration sirtinol on bulk BfCs, coupled with distinct mobility patterns in MNase-digested and psoralen-crosslinked nuclei observed on 2D gels, suggest that telomeric nucleosomes (TNs) are the primary histone structures. The absence of a nucleosomal ladder with cDNA probes, the presence of histone H2A and telomere-enriched H3.3 variants, along with the immuno-localization of H3 variants mainly at the NE further reinforce telomeric regions as the main nucleosomal domains. Cumulative biochemical and molecular analyses suggest that telomeric repeats constitute the major octameric MNRPs that provision chromosomal anchorage at the NE. Full article
(This article belongs to the Section Macromolecules)
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<p>Fluorescent photomicrographs of isolated <span class="html-italic">Karenia</span> nuclei and chromosomes exhibiting differential decompaction between the two termini. (<b>A</b>) Fluorescent photomicrograph of freshly prepared <span class="html-italic">Karenia</span> nuclei examined with SYTOX Green, revealing well-separated chromosomes with unstainable peripheral chromosome loops. Certain dyes were less effective at staining the chromosomes, possibly because their intercalation led to increased decompaction. G<sub>1</sub> cell—white dashed rectangle, G<sub>2</sub> cell—pink dashed rectangle. (<b>B</b>) Chromosome preparations stained with DAPI showed notable differences at the chromosome ends, with the condensed ends marked by red arrows. Several isolated chromosomes appeared to lack a condensed domain at either end (green arrows). (<b>C</b>) After digestion with micrococcal nuclease, most BfCs were digested, leaving behind residual dots (indicated by red arrows). A smaller BfC fraction did not stain well with DAPI, exhibiting a green color; these chromosomes were longer and occasionally had sister BfCs joined together (orange arrows), considered to be G<sub>2</sub> BfCs that required duplication prior to segregation. Some dots appeared green, unlike the typical blue of DAPI-stained DNA. We interpret these green signals as membrane lipids associated with telomeric anchorage to the nuclear envelope. This interpretation is based on several factors: their resistance to nuclease digestion, which suggests a non-DNA composition; the shift from blue to green fluorescence, which is consistent with DAPI’s known interaction with lipids [<a href="#B65-ijms-25-11312" class="html-bibr">65</a>]; and their localization, which aligns with the expected position of telomere attachment sites on the nuclear envelope. While further investigation using specific lipid stains and telomere probes would be beneficial to confirm this interpretation, the observed characteristics are consistent with lipid-rich structures. The chromosomal material that remained resistant (indicated by orange arrow) is thought to represent mitotic anaphase BfCs with a modified chromosome surface. The less-stained chromosome ends, with the proposed anaphase resistance domain, suggested either reduced accessibility or compaction of the mitotic telomeric regions. (<b>D</b>) Transmission electron microscopy of a <span class="html-italic">Karenia</span> nuclear section revealed chromosome ends (black arrow) on the nuclear envelope. Not all DNA dyes, which differed in their effects on DNA structures, resulted in staining enclaves on the NE. For (<b>A</b>–<b>C</b>), scale bar = 10 μm.</p>
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<p>Comparative micrococcal nuclease profiling suggesting that two chromosome termini have rDNA and telomeric repeats with different resistance. (<b>A</b>,<b>B</b>) Semi-automatic polarized light imaging (Metripol) of isolated nuclei suggesting non-nucleic acid domains (black, indicated by white arrow) at chromosome termini in the nuclear envelope that persisted after cation chelation. Chromosome decompaction was induced by treating the nuclei with (<b>B</b>) 2 mM EDTA. The orientation micrographs depict extinction angles, which correspond to the angle between the long axis of the chromosomes and the alignment direction of the chromatin filament fibers. Black arrow points to one chromosome end expulsed from the NE. Scale bar = 10 μm. (<b>C</b>) Ethidium bromide staining and telomeric Southern blot analysis of <span class="html-italic">K. brevis</span> nuclei subjected to MNase digestion following restriction enzyme treatment (25 U MboI digestion of 10<sup>5</sup> nuclei). (<b>D</b>) MNase-digested isolated chromosome preparation, and (<b>E</b>) MNase-digested nuclei preparation. The isolated chromosomes did not exhibit the prominent MNase-protected domains that the whole nuclei did (red boxes, <a href="#ijms-25-11312-f002" class="html-fig">Figure 2</a>D compared to <a href="#ijms-25-11312-f002" class="html-fig">Figure 2</a>E), with apparent m.w. lower than 100 bp, and the protected domain remained largely undigested, even after 24 h (compared to digested nuclei in <a href="#ijms-25-11312-f002" class="html-fig">Figure 2</a>E, green box region). (<b>F</b>) Ethidium bromide staining and 16S rDNA Southern blot analysis of MNase-digested <span class="html-italic">K. brevis</span> nuclei. The high-molecular-weight rDNA in the open-end (red box) did not share the resistance pattern of telomeric repeats (<b>E</b>), indicating that some of the open-ended rDNAs were in a nucleosomal conformation that persisted for up to 5 h of MNase digestion. MNase-protected domains, revealed by overnight digestion (24 h) and probed with rDNA, indicated the presence of persistently protected rDNA loci, implicating potential protection with nucleosomal domains. Two distinct rDNA (rRNA)-associated populations are highlighted in yellow (<b>F</b>), with equal intensities, suggesting that the population associated with the gel was not a result of digesting the well-associated population.</p>
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<p>High-resolution analysis of micrococcal nuclease-resistant regions revealing DNA–protein interactions and chromosomal anchoring structures. (<b>A</b>) Diagrammatic representation of micrococcal nuclease-mediated BfC disassembly in the absence of restriction enzymes. BfCs of dinoflagellates are hypothesized to organize into three distinct compartments [<a href="#B63-ijms-25-11312" class="html-bibr">63</a>]: Compartment 1 (C(i)) is the highly condensed inner core with a columnar–hexagonal mesophase, exhibiting the highest DNA packaging density. Compartment 2 (C(ii.1)) is the less dense surface layer that spirals around C(i), consisting of chromonema coils. Compartment 3 (C(ii.2)) forms the outermost layer, comprising peripheral chromosomal loops (PCLs) that are transcriptionally active. C(ii.1) and C(ii.2) exist in a dynamic equilibrium, with C(ii.2) capable of condensing into C(ii.1), especially during mitosis. This compartmental organization allows for unique structural flexibility and functional regulation through soft-matter phase transitions, which are crucial for the distinctive chromosome architecture and function in dinoflagellates [<a href="#B63-ijms-25-11312" class="html-bibr">63</a>]. (<b>B–D</b>) Pre-digestion of additional restriction enzymes led to faster emergence of the higher-sensitivity fraction and more resolved higher resilient fractions. (<b>B</b>) Isolated <span class="html-italic">Karenia</span> nuclei were digested with PstI (CTGCA^G) and MboI (^GATC) at 37 °C for 1 h prior to gel electrophoresis. (<b>C</b>) <span class="html-italic">Karenia brevis</span> nuclei digested with MboI were subsequently subjected to MNase digestion for 1 h using various amounts of the enzyme (enzyme units). (<b>D</b>) Southern blotting of the samples from (<b>C</b>), using telomeric sequences as the probe. Telomeric positive resilient fractions were observed at lower MNase amounts (0.5–10 units). We interpret this as potentially indicative of mitotic chromosomes with highly condensed domains. The increasing amount of telomeric Southern signal, rather than decreasing as expected for open accessibility, suggested that the NE-associated chromosome ends were only accessible after concerted RE + micrococcal nuclease digestion (red arrow). (<b>E</b>) Deproteination of the DNase I-digested sample resulted in a nucleosome-like ladder pattern (green box) on the telomeric Southern blot. (<b>F</b>) Diagrammatic representation of micrococcal nuclease-mediated BfC disassembly with restriction enzymes. Southern blotting analysis of <span class="html-italic">C. cohnii</span> nuclei MNase profiling using probes specific to (<b>G</b>) repetitive elements Cc18 and (<b>H</b>) Cc20.</p>
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<p>Sirtinol-mediated chromosome decompaction leading to telosomal enclave expansion within nuclear envelope. Sirtinol treatment resulted in the decompaction of chromosome termini, accompanied by the formation of swollen-like nodules on the nuclear envelope. (<b>A</b>) Confocal images of anti-H3K9me3-labeled <span class="html-italic">Karenia brevis</span> nuclei. DAPI-stained DNA was pseudo-colored red. Although no apparent labeling was observed at the nuclear center, the nuclear envelope (NE) may have been displaced during post-fixation, resulting in the appearance of a thicker NE. (<b>B</b>) Immunoblot analysis of the H3K9me3 epigenetic mark in <span class="html-italic">Karenia brevis</span> cells treated with 100 μM sirtinol. (<b>C</b>) Sirtinol-induced decompaction of chromosome termini. After 9 h of treatment with 100 µM sirtinol, the nuclear volume significantly increased, ballooning to the size of the cells. (<b>D</b>) At 100 µm sirtinol, individual BfCs, initially tightly packed, became visible. By T = 9 h, BfCs lost their stainability, except at the nuclear envelope, where swollen-like nodules were observed. All the samples were stained with SYTOX Green. (<b>E</b>) Comparison of control and AMSA-treated isolated nuclei: Control, 2 h AMSA treatment, 4 h AMSA treatment, and 6 h AMSA treatment. AMSA-induced BfCs exhibited much greater decompaction, without individualized BfCs being visualized, and no SYTOX Green staining was observed on the nuclear envelope. This suggests that the decompaction of BfCs mediated by the topoisomerase II inhibitor AMSA progressed from the chromosome proper, whereas sirtinol-induced decompaction progressed from the nuclear envelope. It is noteworthy that sirtinol required a significantly longer time for decompaction compared with AMSA, with centrally located BfCs taking a longer time to decompact (blue arrows). Scale bar = 10 μm.</p>
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<p>H3K9me3 reduction suppressed the exit of the S phase and cell proliferation. (<b>A</b>) Chaetocin treatment was associated with reduced cell proliferation. Data represent means ± SE of triplicate experiments. Asterisks (*) indicate significant differences from the control (<span class="html-italic">p</span> &lt; 0.05). (<b>B</b>) Immunoblot analyses of core histones in chaetocin-treated <span class="html-italic">C. cohnii</span> cells confirming the inhibition of H3K9me3. (<b>C</b>) DNA-flow cytograms of synchronized <span class="html-italic">Crypthecodinium cohnii</span> cells treated with chaetocin (administered at T = 0). Chaetocin, a histone methyltransferase inhibitor specifically targeting histone H3K9 tri-methylation (H3K9me3), induced a delay in S-phase exit (or G<sub>2</sub> phase entry) in treated cells compared with the vehicle control (0.01% <span class="html-italic">v</span>/<span class="html-italic">v</span> DMSO), evidenced by the gradual shift of twin peaks toward the G<sub>1</sub> phase.</p>
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<p>Telomeric nucleosomal domains are the major core histone-containing fraction in birefringent chromosomes. (<b>A</b>) Ethidium bromide (EtBr) staining and (<b>B</b>) telomeric Southern blot analysis were conducted on 2D gels analyzing the MNase-digested <span class="html-italic">K. brevis</span> nuclei and (<b>C</b>,<b>D</b>) psoralen-crosslinked, MNase-digested <span class="html-italic">K. brevis</span> nuclei. Psoralen crosslinking led to a complete shift in resistance of all telomeric sequences to MNase digestion. (<b>E</b>–<b>I</b>) Overlay images of the EtBr-stained gels and telomeric Southern blots. Psoralen pre-crosslinking caused a significant shift in nearly all resistant DNAs, including two populations of telomeric-containing DNA. The higher-molecular-weight fraction, which was stained with EtBr, indicated the presence of dsDNA. The EtBr-negative fraction may consist of ssDNA at lower concentrations. Both populations shifted to the same position following psoralen treatment, suggesting their association with proteins. The majority of the telomeric repeat-positive range exhibited a linear relationship, indicating a dose-dependent effect. Most of the supercoiled DNA exhibited resistance to MNase digestion and contained telomeric sequences. The substantial changes in mobility demonstrated the lesser superhelicity commonly associated with telomeric nucleosomes. (<b>J</b>) Workflow illustrating the procedure for MNase digestion and psoralen crosslinking of <span class="html-italic">K. brevis</span> nuclei.</p>
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<p>Psoralen pre-crosslinking led to a substantial increase in immunocaptured micrococcal nuclease-resistant nucleosome–octameric complexes. (<b>A</b>) Immunoblot analysis of histone H2B in the CcH2A-immunoprecipitates pulled down from DSG–formaldehyde or DSG–psoralen-crosslinked <span class="html-italic">Crypthecodinium cohnii</span> cell lysates. (<b>B</b>) Immunoblot analysis of histone H3 in the same immunoprecipitants as (<b>A</b>). Samples were analyzed on denaturing SDS-PAGE gels. The use of psoralen coupled with DSG-crosslinking, when compared with DSG–formaldehyde crosslinking alone, was more efficient in pulling down high-molecular-weight bands/complexes (yellow boxes) that were absent in the mock control. These high-molecular-weight bands represent crosslinked protein complexes that were not fully dissociated under the denaturing conditions. Lower-molecular-weight bands/complexes (orange boxes), likely corresponding to H2A–H2B or H3–H4 complexes, were also detected in the IP product. (<b>C</b>) Workflow diagram for (<b>A</b>,<b>B</b>). (<b>D</b>) EtBr staining of electrophoresed Psoralen-crosslinked G<sub>1</sub> and G<sub>2</sub> <span class="html-italic">C. cohnii</span> nuclei preparations. (<b>E</b>) Silver staining of the excised ~100–1000 bp fraction (from (<b>D</b>)) following SDS-PAGE. Notably, the apparent molecular weight (mw) of several kilobase pairs shifted from the monomeric 100–200 bp DNA (without proteins) to approximately 500 bp of protein–DNA complex within a nucleosome. The free population with open ends exhibited a streaky and continuous distribution, while the non-open end displayed higher integrity. (<b>F</b>) Workflow diagram for (<b>D</b>,<b>E</b>).</p>
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20 pages, 4833 KiB  
Article
The Downregulation of the Liver Lipid Metabolism Induced by Hypothyroidism in Male Mice: Metabolic Flexibility Favors Compensatory Mechanisms in White Adipose Tissue
by Lamis Chamas, Isabelle Seugnet, Odessa Tanvé, Valérie Enderlin and Marie-Stéphanie Clerget-Froidevaux
Int. J. Mol. Sci. 2024, 25(19), 10792; https://doi.org/10.3390/ijms251910792 - 8 Oct 2024
Viewed by 668
Abstract
In mammals, the maintenance of energy homeostasis relies on complex mechanisms requiring tight synchronization between peripheral organs and the brain. Thyroid hormones (THs), through their pleiotropic actions, play a central role in these regulations. Hypothyroidism, which is characterized by low circulating TH levels, [...] Read more.
In mammals, the maintenance of energy homeostasis relies on complex mechanisms requiring tight synchronization between peripheral organs and the brain. Thyroid hormones (THs), through their pleiotropic actions, play a central role in these regulations. Hypothyroidism, which is characterized by low circulating TH levels, slows down the metabolism, which leads to a reduction in energy expenditure as well as in lipid and glucose metabolism. The objective of this study was to evaluate whether the metabolic deregulations induced by hypothyroidism could be avoided through regulatory mechanisms involved in metabolic flexibility. To this end, the response to induced hypothyroidism was compared in males from two mouse strains, the wild-derived WSB/EiJ mouse strain characterized by a diet-induced obesity (DIO) resistance due to its high metabolic flexibility phenotype and C57BL/6J mice, which are prone to DIO. The results show that propylthiouracil (PTU)-induced hypothyroidism led to metabolic deregulations, particularly a reduction in hepatic lipid synthesis in both strains. Furthermore, in contrast to the C57BL/6J mice, the WSB/EiJ mice were resistant to the metabolic dysregulations induced by hypothyroidism, mainly through enhanced lipid metabolism in their adipose tissue. Indeed, WSB/EiJ mice compensated for the decrease in hepatic lipid synthesis by mobilizing lipid reserves from white adipose tissue. Gene expression analysis revealed that hypothyroidism stimulated the hypothalamic orexigenic circuit in both strains, but there was unchanged melanocortin 4 receptor (Mc4r) and leptin receptor (LepR) expression in the hypothyroid WSB/EiJ mice strain, which reflects their adaptability to maintain their body weight, in contrast to C57BL/6J mice. Thus, this study showed that WSB/EiJ male mice displayed a resistance to the metabolic dysregulations induced by hypothyroidism through compensatory mechanisms. This highlights the importance of metabolic flexibility in the ability to adapt to disturbed circulating TH levels. Full article
(This article belongs to the Special Issue Metabolism and Diseases Related to Thyroid Function)
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Figure 1
<p>PTU treatment effects on metabolism including body weight, food intake and body fat mass. (<b>A</b>) Body weight variation upon weeks of treatment expressed as a percentage of the starting body weight (Week 0) for each group (% each week BW/Initial BW before the treatment; <span class="html-italic">n</span> = 13–14 per group). (<b>B</b>) Food intake measurements (relative to BW) during the 7 weeks of treatment (<span class="html-italic">n</span> = 13–15 per group). Food intake data were collected starting from 24 h before the treatment (−1). (<b>A</b>,<b>B</b>) The statistical differences between treatment and time were assessed by two-way repeated measures ANOVA followed by Tukey’s multiple-comparisons test. Significant differences were indicated by different symbols to account for group differences at each week (blue and pink *: WSB/EIJ CTRL vs. PTU and C57BL/6J CTRL vs. PTU, respectively; <span>$</span>: C57BL/6J CTRL vs. WSB/EIJ CTRL; #: C57BL/6J PTU vs. WSB/EIJ PTU; *,#, <span class="html-italic">p</span> ≤ 0.05; ##, <span class="html-italic">p</span> ≤ 0.01; ###, <span class="html-italic">p</span> ≤ 0.001; ####, <span>$</span><span>$</span><span>$</span><span>$</span>, <span class="html-italic">p</span> ≤ 0.0001). Values are mean ± SEM. (<b>C</b>) Effect of hypothyroidism on bone growth. Femur length measurements were not affected by PTU treatment in C57BL/6J and WSB/EiJ mice (<span class="html-italic">n</span> = 8–12 per group; non-parametric two-way ANOVA with permutations test, <span class="html-italic">p</span> &gt; 0.05). (<b>D</b>) Epididymal white adipose tissue (eWAT) weights measured at the end of the treatment (relative to final BW; <span class="html-italic">n</span> = 12–15 per group; non-parametric one-way ANOVA with permutations test). (<b>E</b>) Circulating leptin (in ng/mL; <span class="html-italic">n</span> = 9–13 per group) Boxplot represents median values and min–max whiskers. Non-parametric two-way ANOVA with permutations test were performed for (<b>E</b>) data (see <a href="#app1-ijms-25-10792" class="html-app">Table S2</a>). Statistically significant effects with the respective <span class="html-italic">p</span>-values are indicated on the graph (S: strain, S × T: strain × treatment interaction). Post hoc tests results are indicated on the graphs (**, <span class="html-italic">p</span> ≤ 0.01; ***, <span class="html-italic">p</span> ≤ 0.001; ****, <span class="html-italic">p</span> ≤ 0.0001).</p>
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<p>PTU treatment effect on hypothalamic control of energy balance. Expression of appetite-stimulating (orexigenic) neuropeptide agouti-related peptide (<span class="html-italic">Agrp</span>) (<b>A</b>), appetite-suppressing (anorexigenic) neuropeptides pro-opiomelanocortin (<span class="html-italic">Pomc</span>) (<b>B</b>), melanocortin 4 receptor (<span class="html-italic">Mc4r)</span> (<b>C</b>) and leptin receptor (<span class="html-italic">Lepr</span>) (<b>D</b>) in response to 7weeks of PTU treatment. Data are represented as relative fold-change expression (FC). Boxplots represent median values and min–max whiskers. Non-parametric one-way ANOVA with permutations post hoc tests results are indicated on the graph (<span class="html-italic">n</span> = 5–6 per group; *, <span class="html-italic">p</span> ≤ 0.05; **, <span class="html-italic">p</span> ≤ 0.01; nqs: not quite significant). Non-parametric two-way ANOVA with permutations test: statistically significant effects with the respective <span class="html-italic">p</span>-values are indicated on the graph (S: strain, T: treatment, S × T: strain × treatment interaction). When interaction <span class="html-italic">p</span>-value is significant, post hoc test results are indicated on the figure.</p>
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<p>Circulating lipid patterns are differentially altered between mouse strains in response to hypothyroidism. (<b>A</b>) Cholesterol (in mmol/L), (<b>B</b>) low-density lipoprotein fatty acid (LDL in mmol/L) and (<b>C</b>) high-density lipoprotein (HDL in mmol/L) levels were increased in both mouse <span class="html-italic">p</span>-values as indicated on the graph (S: strain, T: treatment, S × T: strain × treatment interaction). Strains after PTU treatment. (<b>D</b>) Triglycerides (in mmol/L) and (<b>E</b>) non-esterified fatty acid (NEFA in mmol/L) levels were decreased in hypothyroid C57BL/6J mice whereas they remained unchanged in WSB/EiJ mice. Boxplot represents median values and min–max whiskers. Non-parametric one-way ANOVA with permutations post hoc tests results are indicated on the graph (<span class="html-italic">n</span> = 7–8 per group; *, <span class="html-italic">p</span> ≤ 0.05; **, <span class="html-italic">p</span> ≤ 0.01; ***, <span class="html-italic">p</span> ≤ 0.001). Non-parametric two-way ANOVA with permutations test: statistically significant effects with the respective <span class="html-italic">p</span>-values are indicated on the graph (S: strain, T: treatment, S × T: strain × treatment interaction). When interaction <span class="html-italic">p</span>-value is significant, post hoc test results are indicated on the figure.</p>
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<p>Hepatic lipid metabolism is reduced in both strains in response to hypothyroidism. Hepatic expression of lipogenesis key genes <span class="html-italic">Fasn</span> (<b>A</b>) and <span class="html-italic">Acacα</span> (<b>B</b>) involved in the fatty acid and TGs synthesis (referred as de novo lipogenesis (DNL)) were downregulated in both strains after PTU treatment as well as <span class="html-italic">Chrebp</span> expression (<b>C</b>), the DNL-regulated transcription factor. Expression of <span class="html-italic">Pparα</span> (<b>D</b>), <span class="html-italic">Ppargc1α</span> (<b>E</b>) and <span class="html-italic">Fgf21</span> (<b>F</b>) genes involved in lipid metabolism regulation and fatty acid oxidation were differentially regulated between strains. Data are represented as relative fold-change expression (FC). Boxplot represents median values and min–max whiskers. Non-parametric two-way ANOVA with permutations tests: statistically significant effects with the respective <span class="html-italic">p</span>-values are indicated on the graph (S: strain, T: treatment, S × T: strain × treatment interaction). When the interaction <span class="html-italic">p</span>-value is significant, post hoc test results are indicated on the figure (<span class="html-italic">n</span> = 5–6 per group; *, <span class="html-italic">p</span> ≤ 0.05; **, <span class="html-italic">p</span> ≤ 0.01).</p>
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<p>Adipose lipid metabolism is globally enhanced in WSB/EiJ mice and unchanged in C57BL/6J mice under PTU treatment. (<b>A</b>) Expression of <span class="html-italic">Pnpla2</span> transcript, catalyzing TGs into fatty acids (referred as lipolysis) seemed to be increased only in WSB/EiJ mice but did not reach significance. (<b>B</b>) Expression of <span class="html-italic">Ppargc1α</span> gene, involved in fatty acid oxidation, was downregulated between strains. Expression in the eWAT of lipogenesis key genes <span class="html-italic">Fasn</span> (<b>C</b>) and <span class="html-italic">Acacα</span> (<b>D</b>) involved in the fatty acid and TGs synthesis was upregulated only in WSB/EiJ mice after PTU treatment. (<b>E</b>) Expression of the DNL-regulated transcription factor <span class="html-italic">Pparγ</span> gene was different between strains. Data are represented as relative fold-change expression (FC). Boxplot represents median values with min–max whiskers. Non-parametric two-way ANOVA with permutations tests: statistically significant effects with the respective <span class="html-italic">p</span>-values are indicated on the graph (S: strain, T: treatment, S × T: strain × treatment interaction). When interaction <span class="html-italic">p</span>-value is significant, post hoc test results are indicated on the figure (<span class="html-italic">n</span> = 5–6 per group; *, <span class="html-italic">p</span> ≤ 0.05; **, <span class="html-italic">p</span> ≤ 0.01; ***, <span class="html-italic">p</span> ≤ 0.001).</p>
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<p>Effect of PTU treatment on inflammatory cytokines. (<b>A</b>) Expression of pro-inflammatory <span class="html-italic">Il1B</span>, <span class="html-italic">Il6</span>, <span class="html-italic">Tnfα</span>, and anti-inflammatory <span class="html-italic">Il10</span> cytokine genes in the eWAT were overall downregulated in hypothyroid C57BL/6J mice, whereas they remained unchanged in WSB/EiJ mice. Data are represented as relative fold-change expression (FC). (<b>B</b>) Circulating inflammatory cytokines measured by ELISA: IL10 (in pg/mL), IL1β (in pg/mL), IFNγ (in pg/mL), and IL6 (in pg/mL) levels were overall decreased in hypothyroid C57BL/6J mice, whereas they remained unchanged in WSB/EiJ mice. Boxplot represents median values with min–max whiskers. Non-parametric one-way ANOVA with permutations post hoc tests results are indicated on the graph (<span class="html-italic">n</span> = 5–6 per group; *, <span class="html-italic">p</span> ≤ 0.05; **, <span class="html-italic">p</span> ≤ 0.01; ***, <span class="html-italic">p</span> ≤ 0.001; <span class="html-italic">ns</span>, not significant). Non-parametric two-way ANOVA with permutations tests: statistically significant effects with the respective <span class="html-italic">p</span>-values are indicated on the graph (S: strain, T: treatment, S × T: strain × treatment interaction). When interaction <span class="html-italic">p</span>-value is significant, post hoc test results are indicated on the figure.</p>
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<p>Effect of hypothyroidism on glial cells density in the hypothalamic arcuate nucleus (ARC) of mouse strains. (<b>A</b>) Representative confocal images of IBA1+ microglia (green), in the ARC (white ROI) of euthyroid (CTRL) and hypothyroid (PTU) C57BL/6J (left panel) and WSB/EiJ (right panel) mice. Cells nuclei are stained with DAPI (in blue). (<b>B</b>) Quantitative analysis revealed a decrease in microglia density in the ARC of C57BL/6J mice, whereas it was unchanged in WSB/EiJ mice in response to PTU treatment. (<b>C</b>) Representative confocal images of GFAP+ astrocytes (red), in the ARC of euthyroid (CTRL) and hypothyroid (PTU) C57BL/6J (left panel) and WSB/EiJ (right panel) mice. Cells nuclei are stained with DAPI (in blue). (<b>D</b>) Density of GFAP+ astrocytes remained unchanged in the ARC of both mouse strains after PTU treatment, and a strain effect revealed lower astrocyte density in the ARC of WSB/EiJ compared to C57BL/6J mice. Boxplot represents median values with min–max whiskers. Strain effect result is indicated on the graph (<span class="html-italic">n</span> = 4 mice per group, <span class="html-italic">n</span> = 2–6 sections per mouse and <span class="html-italic">n</span> = 2 ROI per section). Non-parametric two-way ANOVA with permutations tests: statistically significant effects with the respective <span class="html-italic">p</span>-values are indicated on the graph (S: strain, T: treatment, S × T: strain × treatment interaction). When the interaction <span class="html-italic">p</span>-value is significant, post hoc test results are indicated on the figure. *, <span class="html-italic">p</span> ≤ 0.05; **, <span class="html-italic">p</span> ≤ 0.01; ***, <span class="html-italic">p</span> ≤ 0.001. Scale bars = 100 μm.</p>
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<p>Metabolic flexibility allows for adaptation to circulating hypothyroidism: Circulating hypothyroidism induces different consequences on peripheral lipid metabolism in C57BL/6J and WSB/EiJ mouse strains. In C57BL/6J mice (<b>left</b>) and WSB/EiJ mice (<b>right</b>), circulating hypothyroidism induces an alteration in hepatic lipid metabolism, resulting in a decreased lipid synthesis in the liver. However, compensatory mechanisms (mobilization of WAT lipid stores) occur only in WSB/EiJ mice to maintain their circulating levels of NEFA and TG despite hypothyroidism, and therefore prevent hypothyroidism-induced weight loss, unlike in C57BL/6J mice. These results highlight the importance of metabolic flexibility in order to adapt to TH level disruption. <span class="html-italic">This figure was created with BioRender.com.</span></p>
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24 pages, 7675 KiB  
Article
Coordinated Ship Welding with Optimal Lazy Robot Ratio and Energy Consumption via Reinforcement Learning
by Rui Yu and Yang-Yang Chen
J. Mar. Sci. Eng. 2024, 12(10), 1765; https://doi.org/10.3390/jmse12101765 - 5 Oct 2024
Viewed by 502
Abstract
Ship welding is a crucial part of ship building, requiring higher levels of robot coordination and working efficiency than ever before. To this end, this paper studies the coordinated ship-welding task, which involves multi-robot welding of multiple weld lines consisting of synchronous ones [...] Read more.
Ship welding is a crucial part of ship building, requiring higher levels of robot coordination and working efficiency than ever before. To this end, this paper studies the coordinated ship-welding task, which involves multi-robot welding of multiple weld lines consisting of synchronous ones to be executed by a pair of robots and normal ones that can be executed by one robot. To evaluate working efficiency, the objectives of optimal lazy robot ratio and energy consumption were considered, which are tackled by the proposed dynamic Kuhn–Munkres-based model-free policy gradient (DKM-MFPG) reinforcement learning algorithm. In DKM-MFPG, a dynamic Kuhn–Munkres (DKM) dispatcher is designed based on weld line and co-welding robot position information obtained by the wireless sensors, such that robots always have dispatched weld lines in real-time and the lazy robot ratio is 0. Simultaneously, a model-free policy gradient (MFPG) based on reinforcement learning is designed to achieve the energy-optimal motion control for all robots. The optimal lazy robot ratio of the DKM dispatcher and the network convergence of MFPG are theoretically analyzed. Furthermore, the performance of DKM-MFPG is simulated with variant settings of welding scenarios and compared with baseline optimization methods. Compared to the four baselines, DKM-MFPG owns a slight performance advantage within 1% on energy consumption and reduces the average lazy robot ratio by 11.30%, 10.99%, 8.27%, and 10.39%. Full article
(This article belongs to the Special Issue Ship Wireless Sensor)
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<p>An example of the coordinated ship-welding task.</p>
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<p>Motion of robots on the gantry.</p>
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<p>The DKM-MFPG framework.</p>
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<p>An example of the DKM dispatcher for Case 2.</p>
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<p>Trajectories of robots for S1–S5 under DKM-MFPG.</p>
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<p>Trajectories of robots for S1–S5 under DKM-MFPG.</p>
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<p>Number of non-lazy robots for S1–S5 under DKM-MFPG.</p>
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<p>Time histories of state, action, energy, and weights of DKM-MFPG under S1–S5.</p>
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<p>Pareto front of all methods under S1–S5.</p>
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<p>Pareto front of all methods under S1–S5.</p>
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36 pages, 14602 KiB  
Article
Reliability Enhancement of a Double-Switch Single-Ended Primary Inductance–Buck Regulator in a Wind-Driven Permanent Magnet Synchronous Generator Using a Double-Band Hysteresis Current Controller
by Walid Emar, Mais Alzgool and Ibrahim Mansour
Energies 2024, 17(19), 4868; https://doi.org/10.3390/en17194868 - 27 Sep 2024
Viewed by 464
Abstract
The wind power exchange system (WECS) covered in this paper consists of a voltage source inverter (VSI), a DSSB regulator, and an uncontrolled rectifier. An AC grid or a heavy inductive or resistive load (RL) can be supplied by this system. The DSSB [...] Read more.
The wind power exchange system (WECS) covered in this paper consists of a voltage source inverter (VSI), a DSSB regulator, and an uncontrolled rectifier. An AC grid or a heavy inductive or resistive load (RL) can be supplied by this system. The DSSB is a recently developed DC-DC regulator consisting of an improved single-ended primary inductance regulator (SEPIC) followed by a buck regulator. It has a peak efficiency of 95–98% and a voltage gain of (D (1+D)/(1D). where D is the regulator transistor’s on-to-off switching ratio. The proposed regulator improves the voltage stability and MPPT strategy (optimal or maximum power-point tracking). The combination of the DSSB and the proposed regulator improves the efficiency of the system and increases the power output of the wind turbine by reducing the harmonics of the system voltages and current. This method also reduces the influence of air density as well as wind speed variations on the MPPT strategy. Classical proportional–integral (PI) controllers are used in conjunction with a vector-controlled voltage source inverter, which adheres to the suggested DSSB regulator, to control the PMSM speed and d-q axis currents and to correct for current error. In addition to the vector-controlled voltage source inverter (which follows the recommended DSSB regulator), classical proportional–integral controllers are used to regulate the PMSM speed and d-q axis currents, and to correct current errors. In addition, a model Predictive Controller (PPC) is used with the pitch angle control (PAC) of WECS. This is done to show how well the proposed WECS (WECS with DSSB regulator) enhances voltage stability. A software-based simulation (MATLAB/Simulink) evaluates the results for ideal and unoptimized parameters of the WT and WECS under a variety of conditions. The results of the simulation show an increase in MPPT precision and output power performance. Full article
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<p>PMSG-based VSWT with a rectifier and boost DC-DC regulator, Voltage Source Inverter (VSI), and PMSM load.</p>
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<p>Possible connection of DSSB regulator.</p>
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<p>Results of the DSSB MATLAB simulation for <math display="inline"><semantics> <mrow> <mi>D</mi> <mo> </mo> <mo>≤</mo> <mo>(</mo> <msqrt> <mn>2</mn> </msqrt> <mo>−</mo> <mn>1</mn> <mo>)</mo> </mrow> </semantics></math>: (<b>a</b>) waveforms for the DC voltage and current in the input and output of the DSSB under equilibrium conditions. (<b>b</b>) Steady-state waveforms of DSSB currents and voltages. (<b>c</b>) DC input and output powers with a regulator efficiency of 99%.</p>
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<p>Results of the DSSB MATLAB simulation for <math display="inline"><semantics> <mrow> <mi>D</mi> <mo> </mo> <mo>≤</mo> <mo>(</mo> <msqrt> <mn>2</mn> </msqrt> <mo>−</mo> <mn>1</mn> <mo>)</mo> </mrow> </semantics></math>: (<b>a</b>) waveforms for the DC voltage and current in the input and output of the DSSB under equilibrium conditions. (<b>b</b>) Steady-state waveforms of DSSB currents and voltages. (<b>c</b>) DC input and output powers with a regulator efficiency of 99%.</p>
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<p>Results of the DSSB MATLAB simulation for <math display="inline"><semantics> <mrow> <mi>D</mi> <mo> </mo> <mo>≥</mo> <mo>(</mo> <msqrt> <mn>2</mn> </msqrt> <mo>−</mo> <mn>1</mn> <mo>)</mo> </mrow> </semantics></math>: (<b>a</b>,<b>b</b>) Waveforms for the steady-state current and voltage in the DSSB for both the input and output DC voltages and currents. (<b>c</b>) DC active powers of input and output with a 99% regulator efficiency.</p>
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<p>Sketch of a three-phase salient-pole synchronous machine: original system (<b>left</b>), and two-phase replacement (<b>right</b>).</p>
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<p>dq model of PMSG equivalent circuit in the synchronous reference frame.</p>
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<p>Simplified schematic diagram of the simulated WECS system with an AC grid.</p>
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<p>Power ratio (coefficient) versus tip speed.</p>
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<p>The turbine’s mechanical driving power in relation to tip speed ratio and power output at different wind speeds.</p>
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<p>Turbine-generated mechanical power as a function of rotor speed for varying wind speeds.</p>
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<p>Schematic diagram of the hysteresis current-mode-regulated DSSB regulator along with the PMSG wind turbine and three-phase diode bridge rectifier.</p>
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<p>Block diagram of DC link voltage loop control.</p>
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<p>Diagram of the recommended DSSB along with the ramp-hysteresis current-mode control block.</p>
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<p>The block structure of a const. frequency hysteresis current-mode controller with fixed window limits.</p>
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<p>Mean-value block for computing the total average value of capacitor voltages using MATLAB.</p>
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<p>Control scheme to produce reference cycle for MOS1 switch.</p>
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<p>Control scheme to produce reference cycle for MOS2 switch.</p>
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<p>MATLAB/Simulink-built constant frequency of established bandwidth of double-hysteresis current-mode controller framework for MOS1.</p>
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<p>MATLAB/Simulink-built constant frequency of fixed bandwidth of double-hysteresis current-mode controller framework for MOS2.</p>
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<p>Results of MATLAB simulation of DSSB-based hysteresis controller with constant source current (40 A) and step changes in wind speed (12 m/s, 16 m/s, 20 m/s): (<b>a</b>) DSSB output/input voltages and currents. (<b>b</b>) DSSB output/input powers with efficiency and PMSG output active and reactive powers (<span class="html-italic">P<sub>gen</sub></span> &amp; <span class="html-italic">Q<sub>gen</sub></span>). (<b>c</b>) Torques and rotor speed. Target frequency = 10 kHz.</p>
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<p>Results of MATLAB simulation of DSSB-based hysteresis controller with constant source current (40 A) and variable wind speed (12 m/s, 16 m/s, 20 m/s): (<b>a</b>) dq-axis phase voltages (V<sub>ds</sub>, V<sub>qs</sub>) and dq-axis phase current (I<sub>ds</sub>, I<sub>qs</sub>) of PMSG. (<b>b</b>) PMSG stator currents (I<sub>as</sub>, I<sub>bs</sub>, I<sub>cs</sub>) and PMSG dq-axis line voltages (V<sub>dL</sub>, V<sub>qL</sub>). (<b>c</b>) Snapshot of PMSG stator currents (I<sub>as</sub>, I<sub>bs</sub>, I<sub>cs</sub>) and PMSG dq-axis line voltages (V<sub>dL</sub>, V<sub>qL</sub>) at a wind speed of 12 m/s. Target switching frequency = 10 kHz.</p>
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<p>Results of MATLAB simulation of DSSB-based hysteresis controller with constant source current (40 A) and variable wind speed (12 m/s, 16 m/s, 20 m/s): (<b>a</b>) dq-axis phase voltages (V<sub>ds</sub>, V<sub>qs</sub>) and dq-axis phase current (I<sub>ds</sub>, I<sub>qs</sub>) of PMSG. (<b>b</b>) PMSG stator currents (I<sub>as</sub>, I<sub>bs</sub>, I<sub>cs</sub>) and PMSG dq-axis line voltages (V<sub>dL</sub>, V<sub>qL</sub>). (<b>c</b>) Snapshot of PMSG stator currents (I<sub>as</sub>, I<sub>bs</sub>, I<sub>cs</sub>) and PMSG dq-axis line voltages (V<sub>dL</sub>, V<sub>qL</sub>) at a wind speed of 12 m/s. Target switching frequency = 10 kHz.</p>
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<p>Front-loading inverter in grid-connected mode with DSSB, a diode rectifier, and a DC voltage source, E.</p>
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<p>A per-phase T-model of a two-port cable connected between the grid and the VSI.</p>
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<p>Performance of the grid-side inverter that is connected to the grid through LCL Kalman filters, and is subjected to a stepwise variation in the constant DC source of between 1200 and 2400 volts. The PMSG, DSSB regulator, and wind turbine are removed. (<b>a</b>) The phase voltage, V<sub>f</sub> = V<sub>b</sub>, across the LCL filter with the grid, and the line voltage, V<sub>fL</sub>, across two phases of the LCL filters with the grid, in addition to the phase current flowing through these filters. (<b>b</b>) the line voltage (V<sub>gL</sub>) between two grid phases, as well as the grid phase currents and voltages (Vgabc and Igabc), with the VSI DC supply voltage (V<sub>dc</sub>).</p>
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<p>Performance and power capacity of the grid with the VSI after the wind turbine, PMSG, and DSSB were removed, along with a stepwise variation in the DC source of between 1200 and 2400 volts: (<b>a</b>) Active and reactive powers on the inverter side (P<sub>f</sub> and Q<sub>f</sub>,), the grid side (P<sub>g</sub>, and Q<sub>g</sub>), and the LCL filter side (P<sub>c</sub> and Q<sub>c</sub>) (<b>b</b>) dq-axes currents and voltages of the grid.</p>
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<p>Waveforms of WECS variables as responses to the step change in the wind speed profile (10 m/s and 20 m/s): (<b>a</b>) complete waveform: load’s phase currents (<span class="html-italic">I<sub>ga</sub>, I<sub>gb</sub>, I<sub>gc</sub></span>), phase voltages (<span class="html-italic">V<sub>ag</sub>, V<sub>bg</sub>, V<sub>cg</sub></span>), line voltage (<span class="html-italic">V<sub>gL</sub>)</span>, and active and reactive powers (<span class="html-italic">P<sub>g</sub></span> and <span class="html-italic">Q<sub>g</sub></span>); (<b>b</b>) a condensed waveform of the line and phase voltages of VSI together with the wind speed and load phase current; (<b>c</b>) load dq-axis currents and dq-axis voltages.</p>
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<p>Waveforms of WECS variables as responses to the step change in the wind speed profile (10 m/s and 20 m/s): (<b>a</b>) complete waveform: load’s phase currents (<span class="html-italic">I<sub>ga</sub>, I<sub>gb</sub>, I<sub>gc</sub></span>), phase voltages (<span class="html-italic">V<sub>ag</sub>, V<sub>bg</sub>, V<sub>cg</sub></span>), line voltage (<span class="html-italic">V<sub>gL</sub>)</span>, and active and reactive powers (<span class="html-italic">P<sub>g</sub></span> and <span class="html-italic">Q<sub>g</sub></span>); (<b>b</b>) a condensed waveform of the line and phase voltages of VSI together with the wind speed and load phase current; (<b>c</b>) load dq-axis currents and dq-axis voltages.</p>
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<p>Simulation of WECS variables: (<b>a</b>) Active and reactive power of all main WECS system components (AC load, PMSG, DSSB, VSI). (<b>b</b>) DSSB output/input currents and voltages as the wind speed changes from 10 m/s to 20 m/s. (<b>c</b>) DSSB output and input powers, <span class="html-italic">P<sub>s</sub></span> and <span class="html-italic">P<sub>o</sub></span>, and efficiency with PMSG’s active and reactive powers.</p>
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<p>Simulation of WECS variables: (<b>a</b>) Active and reactive power of all main WECS system components (AC load, PMSG, DSSB, VSI). (<b>b</b>) DSSB output/input currents and voltages as the wind speed changes from 10 m/s to 20 m/s. (<b>c</b>) DSSB output and input powers, <span class="html-italic">P<sub>s</sub></span> and <span class="html-italic">P<sub>o</sub></span>, and efficiency with PMSG’s active and reactive powers.</p>
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<p>The WECS wind power system’s performance in the event of a zero-wind-speed fault: (<b>a</b>) PMSG currents and voltages; (<b>b</b>) DSSB currents and voltages; (<b>c</b>) load-side currents and voltages with wind speed; (<b>d</b>) a snapshot of the load-side currents and voltages with wind speed around the fault point.</p>
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<p>Responses of WECS variables (currents, line and phase voltages) to the step change in the wind speed profile from 10 m/s to 20 m/s: (<b>a</b>) waveforms of grid’s phase currents (<span class="html-italic">I<sub>ga</sub>, I<sub>gb</sub>, I<sub>gc</sub></span>), phase voltages (<span class="html-italic">V<sub>ag</sub>, V<sub>bg</sub>, V<sub>cg</sub></span>), line voltage (<span class="html-italic">V<sub>gL</sub>)</span>, and active and reactive powers (<span class="html-italic">P<sub>g</sub></span> and <span class="html-italic">Q<sub>g</sub></span>); (<b>b</b>) a snapshot-waveform of VSI phase and line voltages (<span class="html-italic">V<sub>f</sub>, V<sub>fL</sub></span>) and grid’s phase current (<span class="html-italic">I<sub>g</sub></span>); (<b>c</b>) dq-axis phase currents and voltages of the grid.</p>
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<p>Simulation of WECS variables: (<b>a</b>) DSSB output/input currents and voltages as the wind speed changes from 10 m/s to 20 m/s. (<b>b</b>) DSSB output and input powers, <span class="html-italic">P<sub>s</sub></span> and <span class="html-italic">P<sub>o</sub></span>, and efficiency with PMSG’s active and reactive powers. (<b>c</b>) Active and reactive power of all main WECS system components (grid, PMSG, DSSB, VSI).</p>
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<p>In response to a wind speed change from 10 m/s to 20 m/s, three types of PMSG variable waveforms are shown here: (<b>a</b>) PMSG torques and rotor angular speed; (<b>b</b>) PMSG stator phase current (Igen) and line voltage (VsL) with their dq-axis phase currents and voltages; and (<b>c</b>) a shorter-term snapshot of (<b>b</b>).</p>
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<p>In response to a wind speed change from 10 m/s to 20 m/s, three types of PMSG variable waveforms are shown here: (<b>a</b>) PMSG torques and rotor angular speed; (<b>b</b>) PMSG stator phase current (Igen) and line voltage (VsL) with their dq-axis phase currents and voltages; and (<b>c</b>) a shorter-term snapshot of (<b>b</b>).</p>
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13 pages, 4569 KiB  
Article
End-to-End Electrocardiogram Signal Transformation from Continuous-Wave Radar Signal Using Deep Learning Model with Maximum-Overlap Discrete Wavelet Transform and Adaptive Neuro-Fuzzy Network Layers
by Tae-Wan Kim and Keun-Chang Kwak
Appl. Sci. 2024, 14(19), 8730; https://doi.org/10.3390/app14198730 - 27 Sep 2024
Viewed by 575
Abstract
This paper is concerned with an end-to-end electrocardiogram (ECG) signal transformation from a continuous-wave (CW) radar signal using a specialized deep learning model. For this purpose, the presented deep learning model is designed using convolutional neural networks (CNNs) and bidirectional long short-term memory [...] Read more.
This paper is concerned with an end-to-end electrocardiogram (ECG) signal transformation from a continuous-wave (CW) radar signal using a specialized deep learning model. For this purpose, the presented deep learning model is designed using convolutional neural networks (CNNs) and bidirectional long short-term memory (Bi-LSTM) with a maximum-overlap discrete wavelet transform (MODWT) layer and an adaptive neuro-fuzzy network (ANFN) layer. The proposed method has the advantage of developing existing deep networks and machine learning to reconstruct signals through CW radars to acquire ECG biological information in a non-contact manner. The fully connected (FC) layer of the CNN is replaced by an ANFN layer suitable for resolving black boxes and handling complex nonlinear data. The MODWT layer is activated via discrete wavelet transform frequency decomposition with maximum-overlap to extract ECG-related frequency components from radar signals to generate essential information. In order to evaluate the performance of the proposed model, we use a dataset of clinically recorded vital signs with a synchronized reference sensor signal measured simultaneously. As a result of the experiment, the performance is evaluated by the mean squared error (MSE) between the measured and reconstructed ECG signals. The experimental results reveal that the proposed model shows good performance in comparison to the existing deep learning model. From the performance comparison, we confirm that the ANFN layer preserves the nonlinearity of information received from the model by replacing the fully connected layer used in the conventional deep learning model. Full article
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<p>Overview of the reconstruction of a CW radar signal into an ECG signal.</p>
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<p>Overview of the ECG signal’s reconstruction process using MODWT, deep learning, and ANFN.</p>
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<p>Process procedure in the design of the ANFN.</p>
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<p>Plot of demodulated radar signal and synchronized ECG: (<b>a</b>) CW radar samples and (<b>b</b>) synchronized ECG samples.</p>
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<p>Membership function for each input channel.</p>
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<p>Comparison between the signal predicted through MCBF-net and the true signal: (<b>a</b>) reconstructed ECG signal by MCBF-net and (<b>b</b>) actual ECG signal.</p>
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<p>Comparison between the signal predicted through MCBF-net and the true signal: (<b>a</b>) reconstructed ECG signal by MCBF-net and (<b>b</b>) actual ECG signal.</p>
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<p>Prediction performance of the actual ECG signal and the reconstructed ECG signal. (Blue signal: the actual ECG; red signal: the reconstructed ECG; and green signal: the difference between the actual ECG and the reconstructed ECG.)</p>
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<p>Performance comparison by MSE between measured and reconstructed ECG signals.</p>
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28 pages, 12031 KiB  
Article
Key Synchronization Method Based on Negative Databases and Physical Channel State Characteristics of Wireless Sensor Network
by Haoyang Pu, Wen Chen, Hongchao Wang and Shenghong Bao
Sensors 2024, 24(19), 6217; https://doi.org/10.3390/s24196217 - 25 Sep 2024
Viewed by 534
Abstract
Due to their inherent openness, wireless sensor networks (WSNs) are vulnerable to eavesdropping attacks. Addressing the issue of secure Internet Key Exchange (IKE) in the absence of reliable third parties like CA/PKI (Certificate Authority/Public Key Infrastructure) in WSNs, a novel key synchronization method [...] Read more.
Due to their inherent openness, wireless sensor networks (WSNs) are vulnerable to eavesdropping attacks. Addressing the issue of secure Internet Key Exchange (IKE) in the absence of reliable third parties like CA/PKI (Certificate Authority/Public Key Infrastructure) in WSNs, a novel key synchronization method named NDPCS-KS is proposed in the paper. Firstly, through an initial negotiation process, both ends of the main channels generate the same initial key seeds using the Channel State Information (CSI). Subsequently, negotiation keys and a negative database (NDB) are synchronously generated at the two ends based on the initial key seeds. Then, in a second-negotiation process, the NDB is employed to filter the negotiation keys to obtain the keys for encryption. NDPCS-KS reduced the risk of information leakage, since the keys are not directly transmitted over the network, and the eavesdroppers cannot acquire the initial key seeds because of the physical isolation of their eavesdropping channels and the main channels. Furthermore, due to the NP-hard problem of reversing the NDB, even if an attacker obtains the NDB, deducing the initial key seeds is computationally infeasible. Therefore, it becomes exceedingly difficult for attackers to generate legitimate encryption keys without the NDB or initial key seeds. Moreover, a lightweight anti-replay and identity verification mechanism is designed to deal with replay attacks or forgery attacks. Experimental results show that NDPCS-KS has less time overhead and stronger randomness in key generation compared with other methods, and it can effectively counter replay, forgery, and tampering attacks. Full article
(This article belongs to the Section Sensor Networks)
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<p>System model.</p>
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<p>Schematic diagram of the NDB.</p>
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<p>Schematic diagram showing the distribution of the generated NDB and negotiation key in a two-dimensional plane.</p>
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<p>Schematic diagram of communication key generated by dual negotiation of the NDB.</p>
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<p>Data transmission flow.</p>
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<p>Schematic diagram of sensor topology.</p>
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<p>Schematic diagram of ESP32 development board.</p>
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<p>Replay attack detection accuracy.</p>
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<p>(<b>a</b>) Forgery detection accuracy. (<b>b</b>) Tamper detection accuracy.</p>
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<p>Comparison of execution time. The execution time of NDPCS-KS is compared with that of Rangarajan et al. (2023) [<a href="#B18-sensors-24-06217" class="html-bibr">18</a>], Moara-Nkwe et al. (2018) [<a href="#B19-sensors-24-06217" class="html-bibr">19</a>], and Ji et al. (2022) [<a href="#B20-sensors-24-06217" class="html-bibr">20</a>].</p>
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<p>(<b>a</b>) Total key generation time across different network scales. The execution time of NDPCS-KS is compared with the methods of Rangarajan et al. (2023) [<a href="#B18-sensors-24-06217" class="html-bibr">18</a>], Moara-Nkwe et al. (2018) [<a href="#B19-sensors-24-06217" class="html-bibr">19</a>], and Ji et al. (2022) [<a href="#B20-sensors-24-06217" class="html-bibr">20</a>]. (<b>b</b>) Average key generation time per node across different network scales. The comparison includes NDPCS-KS and the methods from Rangarajan et al. (2023) [<a href="#B18-sensors-24-06217" class="html-bibr">18</a>], Moara-Nkwe et al. (2018) [<a href="#B19-sensors-24-06217" class="html-bibr">19</a>], and Ji et al. (2022) [<a href="#B20-sensors-24-06217" class="html-bibr">20</a>].</p>
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<p>Key distribution chart.</p>
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<p>Monte Carlo simulation results.</p>
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<p>Entropy statistics of keys.</p>
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19 pages, 3946 KiB  
Article
Analysis of the Interrelation and Seasonal Variation Characteristics of the Spatial Niche of Dominant Fishery Species—A Case Study of the East China Sea
by Yong Liu and Jiahua Cheng
Biology 2024, 13(9), 751; https://doi.org/10.3390/biology13090751 - 23 Sep 2024
Viewed by 669
Abstract
The spatial niche has garnered significant attention in ecological research, particularly regarding species distribution patterns. The East China Sea, known for its favorable natural conditions and abundant fishery resources, exhibits diverse spatial distribution patterns among species, shaped by their seasonal physiological needs. This [...] Read more.
The spatial niche has garnered significant attention in ecological research, particularly regarding species distribution patterns. The East China Sea, known for its favorable natural conditions and abundant fishery resources, exhibits diverse spatial distribution patterns among species, shaped by their seasonal physiological needs. This study utilized a habitat suitability index model to explore the spatial distribution patterns of key fishery resources in the East China Sea across four seasons and their interactions. Two methodologies were employed to identify key environmental factors and assess the ecological niche overlap among different species and seasons. Results indicated that the initial method identified water temperature as the critical factor for hairtail, while the subsequent method emphasized water temperature and salinity for hairtail, salinity for small yellow croaker, and water depth for Bombay duck. The main spatial habitat overlap was observed between paired species, likely driven by predator-prey interactions. During summer and autumn, increased overlap among multiple species was primarily influenced by synchronized life cycles. An overlap index formula quantified the seasonal species overlap, showing an increase from spring to winter, reflecting changes in convergent habitat preferences. The peak overlap occurred in winter, driven by overwintering, reduced food competition, and enhanced coexistence potential, while the lowest overlap was noted in spring as overwintering ended and predation and competition intensified. Full article
(This article belongs to the Section Ecology)
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<p>Schematic diagram of the study area.</p>
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<p>The relative importance of environmental factors for each fish species by season.</p>
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<p>Interspecies differences in environmental SI by season.</p>
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<p>Seasonal difference of environmental SI of major fish species. Note: The gray area indicates the range where the preferred environmental conditions of different species are relatively consistent.</p>
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<p>Distribution of HSI for each fish species by season.</p>
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<p>The distribution pattern of optimal suitable habitat for each fish species by season.</p>
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<p>Overlapping relationship of optimally suitable habitats for fish species in different seasons. Note: This figure includes four sub-figures, with labels (<b>a</b>–<b>d</b>) in the top left corner of each sub-figure, representing the analysis results for the four seasons: spring, summer, autumn, and winter, respectively.</p>
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25 pages, 4418 KiB  
Article
Two-Stage Optimal Configuration Strategy of Distributed Synchronous Condensers at the Sending End of Large-Scale Wind Power Generation Bases
by Lang Zhao, Zhidong Wang, Yizheng Li, Xueying Wang, Zhiyun Hu and Yunpeng Xiao
Energies 2024, 17(18), 4748; https://doi.org/10.3390/en17184748 - 23 Sep 2024
Viewed by 423
Abstract
The transmission end of large-scale wind power generation bases faces challenges such as high AC-DC coupling strength, low system inertia, and weak voltage support capabilities. Deploying distributed synchronous condensers (SCs) within and around wind farms can effectively provide transient reactive power support, enhance [...] Read more.
The transmission end of large-scale wind power generation bases faces challenges such as high AC-DC coupling strength, low system inertia, and weak voltage support capabilities. Deploying distributed synchronous condensers (SCs) within and around wind farms can effectively provide transient reactive power support, enhance grid system inertia at the transmission end, and improve dynamic frequency support capabilities. However, the high investment and maintenance costs of SCs hinder their large-scale deployment, necessitating the investigation of optimal SC configuration strategies at critical nodes in the transmission grid. Initially, a node inertia model was developed to identify weaknesses in dynamic frequency support, and a critical inertia constraint based on node frequency stability was proposed. Subsequently, a multi-timescale reactive power response model was formulated to quantify the impact on short-circuit ratio improvement and transient overvoltage suppression. Finally, a two-stage optimal configuration strategy for distributed SCs at the transmission end was proposed, considering dynamic frequency support and transient voltage stability. In the first stage, the optimal SC configuration aimed to maximize system inertia improvement per unit investment to meet dynamic frequency support requirements. In the second stage, the configuration results from the first stage were adjusted by incorporating constraints for enhancing the multiple renewable short-circuit ratio (MRSCR) and suppressing transient overvoltage. The proposed model was validated using the feeder grid of a large energy base in western China. The results demonstrate that the optimal configuration scheme effectively suppressed transient overvoltage at the generator end and significantly enhanced the system’s dynamic frequency support strength. Full article
(This article belongs to the Section A3: Wind, Wave and Tidal Energy)
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<p>Sending-end equivalent network of large energy base convergence area.</p>
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<p>Simplified AC equivalent circuit after condenser access.</p>
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<p>Solving process for the two-stage optimal configuration model of distributed SCs in Matlab software (version 2021a).</p>
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<p>Wiring diagram for sending-end grid of large energy bases in China.</p>
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<p>Coupling relationship between economic cost and node inertia enhancement.</p>
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<p>Comparison of node inertia improvement before and after distributed regulator configuration. (<b>a</b>) Before the distributed SC configuration. (<b>b</b>) After the distributed SC configuration.</p>
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<p>Frequency comparison before and after distributed SC configuration.</p>
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<p>Voltage curves of nodes 31 and 36 before and after correction of the distributed SC. (<b>a</b>) The voltage curves of node31 before and after correction of the distributed SC. (<b>b</b>) The voltage curves of node36 before and after correction of the distributed SC.</p>
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<p>Voltage curves of nodes 31 and 36 before and after correction of the distributed SC. (<b>a</b>) The voltage curves of node31 before and after correction of the distributed SC. (<b>b</b>) The voltage curves of node36 before and after correction of the distributed SC.</p>
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<p>Comparison of 42 and 45 transient voltage waveforms. (<b>a</b>) Transient voltage waveform at node 42. (<b>b</b>) Transient voltage waveform at node 45.</p>
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<p>Comparison of 42 and 45 transient voltage waveforms. (<b>a</b>) Transient voltage waveform at node 42. (<b>b</b>) Transient voltage waveform at node 45.</p>
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<p>DC system reactive power output and demand.</p>
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7 pages, 2099 KiB  
Case Report
Synchronous Seminoma of Testis and Renal Cell Carcinoma: A Rare Case Report
by Stasys Auskalnis, Rasa Janciauskiene, Urte Rimsaite, Aurelija Alksnyte and Rasa Ugenskiene
Medicina 2024, 60(9), 1553; https://doi.org/10.3390/medicina60091553 - 23 Sep 2024
Viewed by 802
Abstract
Background and Objectives: Seminoma is the most common solid malignant tumour in young men. Clear-cell kidney carcinoma is the most common malignancy of the genitourinary tract. However, the synchronous occurrence of both of these tumours is rare. Case presentation: We present the [...] Read more.
Background and Objectives: Seminoma is the most common solid malignant tumour in young men. Clear-cell kidney carcinoma is the most common malignancy of the genitourinary tract. However, the synchronous occurrence of both of these tumours is rare. Case presentation: We present the case of a 36-year-old patient who presented to a medical facility at the end of 2019 with an enlarged right testicle. A unilateral orchofuniculectomy was performed, and a mass measuring 30 cm was removed. During histological examination, testicular seminoma pT2, R0, was diagnosed. An abdominal computed tomography (CT) scan showed a 6.4 cm × 6.8 cm × 6.7 cm tumour in the right kidney and a metastatic-like lesion in the right adrenal gland. A right nephrectomy and an adrenalectomy and paraaortic and paracaval lymphadenectomies were performed. A histological evaluation confirmed the presence of clear-cell renal carcinoma pT2aR0 G2, adrenal hyperplasia, and seminoma metastases in the removed lymph node. Chemotherapy with a Bleomycin, Etoposide, and Cisplatin (BEP) regimen was carried out. Three years after the last cycle of chemotherapy, a follow-up CT scan showed metastases in the left kidney, the right ischium, and the right lung. A well-differentiated clear-cell carcinoma G1 of the left kidney and metastasis of clear-cell carcinoma G2 in the right ischium were confirmed after the biopsy, and no tumour lesions were found in the lung tissue specimen. Treatment with targeted therapy with Sunitinib was started because the risk was favourable according to the Heng criteria. Genetic testing was performed, and the following genes were analysed: VHL, BAP1, CHEK2, FH, MET, MUTYH, APC, and STK11. The testing did not reveal any pathogenic or potentially pathogenic mutations or sequence changes of unknown clinical significance in the genes analysed. Conclusions: According to the authors, the occurrence of synchronous primary tumours is linked to one’s genetic predisposition. DNA sequencing of tumour tissue could provide more information on the corresponding aetiopathogenesis. Full article
(This article belongs to the Section Oncology)
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<p>CT image. Pathological paraaortic and paracaval lymph nodes.</p>
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<p>CT image. Tumour mass in the inferior part of the right kidney.</p>
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<p>CT image. Post-right-nephrectomy view. Cyst in the left kidney is shown.</p>
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<p>CT image. Metastatic lesions in the left kidney.</p>
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<p>CT image. Osteoclastic-type metastatic tumour in the pelvic bones.</p>
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<p>CT image. Pathological lymph node with central necrosis in the hilum of the right lung.</p>
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15 pages, 13243 KiB  
Article
Three-Dimensional Probe Mispositioning Errors Compensation: A Feasibility Study in the Non-Redundant Helicoidal Near to Far-Field Transformation Case
by Francesco D’Agostino, Flaminio Ferrara, Claudio Gennarelli, Rocco Guerriero, Massimo Migliozzi, Luigi Pascarella and Giovanni Riccio
Electronics 2024, 13(18), 3767; https://doi.org/10.3390/electronics13183767 - 22 Sep 2024
Viewed by 555
Abstract
A feasibility study on the compensation of 3D mispositioning errors of the probe occurring in the characterization of a long antenna, via a non-redundant (NR) near to far-field (NTFF) transformation with helicoidal scan, is conducted in this article. Such types of errors can [...] Read more.
A feasibility study on the compensation of 3D mispositioning errors of the probe occurring in the characterization of a long antenna, via a non-redundant (NR) near to far-field (NTFF) transformation with helicoidal scan, is conducted in this article. Such types of errors can result from imperfections in the rail driving the linear motion of the probe and from an imprecise synchronization of the linear and rotational movements of the probe and the antenna when drawing the scan helix. To correct them, an approach, which proceeds through two steps, is proposed. The former step uses a technique called cylindo rical wave (CW) correction for compensating the phase of the near-field (NF) samples, which, owing to the rail imperfections, result in not being acquired over the measurement cylinder surface. The latter exploits an iterative scheme to restore the samples at the sampling points required by the adopted NR representation along the scan helix from those obtained by applying the CW correction technique and impaired by 2D mispositioning errors. The so compensated NF samples are then effectively recovered via a 2D optimal sampling interpolation (OSI) scheme to accurately obtain the input data required to carry out the standard cylindrical NTFF transformation. The OSI representation is determined here by assuming a long antenna under test as enclosed in a prolate ellipsoid or cylinder ending into two hemispheres (cigar) in order to make, depending on the particular geometry of the considered antenna, the representation effectively non-redundant. The reported numerical simulation results show the capability of the proposed approach to compensate even severe 3D mispositioning errors, thus enabling its usage in a real measurement scenario. Full article
(This article belongs to the Special Issue Feature Papers in Microwave and Wireless Communications Section)
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<p>Characterization of a long AUT through a helicoidal NF setup.</p>
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<p>(<b>a</b>) Cigar. (<b>b</b>) Prolate ellipsoid.</p>
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<p>Relevant to the determination of <span class="html-italic">μ</span> and <span class="html-italic">ψ</span> in the case of the cigar modeling.</p>
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<p>Representative flowchart of the two-step technique.</p>
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<p>CASE 1. Voltage on the generatrix at <span class="html-italic">φ</span> = 90°. <span style="color:blue">––––</span> exact. <span style="color:red">+ + + +</span> obtained from the mispositioning errors impaired NF samples by using the two-step procedure. • • • • achieved from the positioning errors corrupted NF samples without performing the two-step procedure: (<b>a</b>) Amplitude; (<b>b</b>) Phase.</p>
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<p>CASE 2. Voltage on the generatrix at <span class="html-italic">φ</span> = 90°. <span style="color:blue">––––</span> exact. <span style="color:red">+ + + +</span> obtained from the mispositioning errors impaired NF samples by using the two-step procedure. • • • • achieved from the positioning errors corrupted NF samples without performing the two-step procedure: (<b>a</b>) Amplitude; (<b>b</b>) Phase.</p>
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<p>CASE 1. Voltage on the generatrix at <span class="html-italic">φ</span> = 60°. <span style="color:blue">––––</span> exact. <span style="color:red">+ + + +</span> obtained from the mispositioning errors impaired NF samples by using the two-step procedure. • • • • achieved from the positioning errors corrupted NF samples without performing the two-step procedure: (<b>a</b>) Amplitude; (<b>b</b>) Phase.</p>
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<p>CASE 2. Voltage on the generatrix at <span class="html-italic">φ</span> = 60°. <span style="color:blue">––––</span> exact. <span style="color:red">+ + + +</span> obtained from the mispositioning errors impaired NF samples by using the two-step procedure. • • • • achieved from the positioning errors corrupted NF samples without performing the two-step procedure: (<b>a</b>) Amplitude; (<b>b</b>) Phase.</p>
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<p>Voltage amplitude on the generatrix at <span class="html-italic">φ</span> = 90°. <span style="color:blue">––––</span> exact. <span style="color:red">▼▼▼▼</span> obtained from the positioning errors corrupted NF samples by performing only the CW phase correction: (<b>a</b>) CASE 1; (<b>b</b>) CASE 2.</p>
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<p>Voltage phase on the generatrix at <span class="html-italic">φ</span> = 90°. <span style="color:blue">––––</span> exact. <span style="color:green">◆◆◆◆</span> obtained from the positioning errors NF samples by performing only the iterative procedure: (<b>a</b>) CASE 1; (<b>b</b>) CASE 2.</p>
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<p>Voltage amplitude on the generatrix at <span class="html-italic">φ</span> = 90°. <span style="color:blue">––––</span> exact. <span style="color:red">• • • •</span> obtained from the positioning and measurement errors altered NF samples by using the two-step procedure. (<b>a</b>) CASE 1; (<b>b</b>) CASE 2.</p>
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<p>CASE 1. FF patterns in the principal planes. <span style="color:blue">––––</span> exact. <span style="color:red">+ + + +</span> obtained from the positioning errors corrupted NF samples by using the two-step procedure. • • • • attained from the positioning errors altered NF samples without the two-step procedure: (<b>a</b>) E-Plane; (<b>b</b>) H-plane.</p>
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<p>CASE 2. FF patterns in the principal planes. <span style="color:blue">––––</span> exact. <span style="color:red">+ + + +</span> obtained from the positioning errors corrupted NF samples by using the two-step procedure. • • • • attained from the positioning errors altered NF samples without the two-step procedure: (<b>a</b>) E-Plane; (<b>b</b>) H-plane.</p>
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<p>CASE 1. FF patterns in the principal planes. <span style="color:blue">––––</span> exact. <span style="color:red">▼▼▼▼</span> obtained from the position errors corrupted NF samples using only the CW correction. <span style="color:green">◆◆◆◆</span> obtained from the position errors corrupted NF samples using only the iterative technique. (<b>a</b>) E-Plane; (<b>b</b>) H-plane.</p>
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<p>CASE 2. FF patterns in the principal planes. <span style="color:blue">––––</span> exact. <span style="color:red">▼▼▼▼</span> obtained from the position errors corrupted NF samples using only the CW correction. <span style="color:green">◆◆◆◆</span> obtained from the position errors corrupted NF samples using only the iterative technique. (<b>a</b>) E-Plane; (<b>b</b>) H-plane.</p>
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