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Search Results (1,760)

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Keywords = strain sensing

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14 pages, 4021 KiB  
Article
AI-Aided Gait Analysis with a Wearable Device Featuring a Hydrogel Sensor
by Saima Hasan, Brent G. D’auria, M. A. Parvez Mahmud, Scott D. Adams, John M. Long, Lingxue Kong and Abbas Z. Kouzani
Sensors 2024, 24(22), 7370; https://doi.org/10.3390/s24227370 (registering DOI) - 19 Nov 2024
Abstract
Wearable devices have revolutionized real-time health monitoring, yet challenges persist in enhancing their flexibility, weight, and accuracy. This paper presents the development of a wearable device employing a conductive polyacrylamide–lithium chloride–MXene (PLM) hydrogel sensor, an electronic circuit, and artificial intelligence (AI) for gait [...] Read more.
Wearable devices have revolutionized real-time health monitoring, yet challenges persist in enhancing their flexibility, weight, and accuracy. This paper presents the development of a wearable device employing a conductive polyacrylamide–lithium chloride–MXene (PLM) hydrogel sensor, an electronic circuit, and artificial intelligence (AI) for gait monitoring. The PLM sensor includes tribo-negative polydimethylsiloxane (PDMS) and tribo-positive polyurethane (PU) layers, exhibiting extraordinary stretchability (317% strain) and durability (1000 cycles) while consistently delivering stable electrical signals. The wearable device weighs just 23 g and is strategically affixed to a knee brace, harnessing mechanical energy generated during knee motion which is converted into electrical signals. These signals are digitized and then analyzed using a one-dimensional (1D) convolutional neural network (CNN), achieving an impressive accuracy of 100% for the classification of four distinct gait patterns: standing, walking, jogging, and running. The wearable device demonstrates the potential for lightweight and energy-efficient sensing combined with AI analysis for advanced biomechanical monitoring in sports and healthcare applications. Full article
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<p>Schematic circuit diagram of the wearable device.</p>
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<p>Photographs of the enclosure with the electronic circuit. (<b>a</b>) Enclosed. (<b>b</b>) and (<b>c</b>) Internal components. (<b>d</b>) Weight (19 g).</p>
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<p>Knee brace. (<b>a</b>) Illustration of a human body wearing the knee brace. (<b>b</b>) Photograph of the knee brace with the wearable device. (<b>c</b>) Weight of the wearable device.</p>
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<p>Preparation of the PLM sensor. (<b>a</b>) Preparation of the PLM hydrogel (left); internal morphology of the hydrogel (right). (<b>b</b>) PDMS preparation (left); assembly of the PLM sensor (right).</p>
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<p>Working mechanism of the PLM sensor-based gait monitoring system. (<b>a</b>) Working principle of the PLM sensor during a stretch–release cycle. (<b>b</b>) System-level block diagram of the gait monitoring system, showing analog signals from the four activities (blue), processing and wireless transmission (green), the digital signal output, and the machine learning algorithm run by the computer (yellow).</p>
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<p>Mechanical and electrical performance of the PLM sensor. (<b>a</b>) Tensile stress–strain characteristics. (<b>b</b>) Generated voltage signals with different tensile strains (20%, 40%, 60%, 80%, and 100%). (<b>c</b>) Generated voltage signals at different stretching rates (from 100 to 500 mm min<sup>−1</sup>) at a fixed strain of 80%. (<b>d</b>) Mechanical durability test for up to 1000 continuous stretch–release cycles at 80% strain.</p>
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<p>Gait identification using the 1D CNN model. (<b>a</b>) Voltage acquisition from four gait patterns: standing, walking, jogging, and running. (<b>b</b>) Model structure. (<b>c</b>) Model accuracy. (<b>d</b>) Model loss. (<b>e</b>) Confusion map of the accuracy prediction for the four activities.</p>
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12 pages, 7388 KiB  
Article
Piezoresistive, Piezocapacitive and Memcapacitive Silk Fibroin-Based Cement Mortars
by Daniel A. Triana-Camacho, Antonella D’Alessandro, Silvia Bittolo Bon, Rocco Malaspina, Filippo Ubertini and Luca Valentini
Sensors 2024, 24(22), 7357; https://doi.org/10.3390/s24227357 (registering DOI) - 18 Nov 2024
Viewed by 259
Abstract
Water-stable proteins may offer a new field of applications in smart materials for buildings and infrastructures where hydraulic reactions are involved. In this study, cement mortars modified through water-soluble silk fibroin (SF) are proposed. Water-soluble SF obtained by redissolving SF films in phosphate [...] Read more.
Water-stable proteins may offer a new field of applications in smart materials for buildings and infrastructures where hydraulic reactions are involved. In this study, cement mortars modified through water-soluble silk fibroin (SF) are proposed. Water-soluble SF obtained by redissolving SF films in phosphate buffer solution (PBS) showed the formation of a gel with the β sheet features of silk II. Electrical measurements of SF indicate that calcium ions are primarily involved in the conductivity mechanism. By exploiting the water solubility properties of silk II and Ca2+ ion transport phenomena as well as their trapping effect on water molecules, SF provides piezoresistive and piezocapacitive properties to cement mortars, thus enabling self-sensing of mechanical strain, which is quite attractive in structural health monitoring applications. The SF/cement-based composite introduces a capacitive gauge factor which surpasses the traditional resistive gauge factor reported in the literature by threefold. Cyclic voltammetry measurements demonstrated that the SF/cement mortars possessed memcapacitive behavior for positive potentials near +5 V, which was attributed to an interfacial charge build-up modulated by the SF concentration and the working electrode. Electrical square-biphasic excitation combined with cyclic compressive loads revealed memristive behavior during the unloading stages. These findings, along with the availability and sustainability of SF, pave the way for the design of novel multifunctional materials, particularly for applications in masonry and concrete structures. Full article
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<p>Preparation of specimens based on silk fibroin.</p>
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<p>Experimental set-up to obtain electromechanical properties of SFms.</p>
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<p>Concentration dependence of SF sol–gel transition: (<b>a</b>) dynamic optical morphology, (<b>b</b>) FTIR spectra and (<b>c</b>) relative weights of components obtained by curve–fitting procedure of FTIR spectra of SF prepared by different concentrations.</p>
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<p>(<b>a</b>) Current transients of SF dispersions. (<b>b</b>) Capacitance–RH curve of the prepared SF dispersions with 20%→98%→50% sweeping RH.</p>
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<p>(<b>a</b>) Cyclic voltammetry of SFm specimens with SF concentrations of 0 mg/mL, 50 mg/mL, 100 mg/mL and 200 mg/mL, performed at a scan rate of 100 mV/s. (<b>b</b>) Magnification of SFm specimens with concentrations of 50 mg/mL, 100 mg/mL and 200 mg/mL.</p>
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<p>(<b>a</b>) Electrical resistance and (<b>b</b>) capacitance of SFm samples as a function of the SF concentrations.</p>
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<p>Variation in resistance and capacitance of SFm specimens with SF concentrations of (<b>a</b>,<b>b</b>) 0 mg/mL, (<b>c</b>,<b>d</b>) 50 mg/mL, (<b>e</b>,<b>f</b>) 100 mg/mL and (<b>g</b>,<b>h</b>) 200 mg/mL subjected to cyclic compressive force from 0.02 to 7 kN.</p>
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<p>Correlation between FCR and FCC and compressive strain of SFm specimens with SF concentrations of (<b>a</b>,<b>b</b>) 0 mg/mL, (<b>c</b>,<b>d</b>) 50 mg/mL, (<b>e</b>,<b>f</b>) 100 mg/mL and (<b>g</b>,<b>h</b>) 200 mg/mL. The whole specimens were subjected to cyclic compressive force from 0.02 to 7 kN.</p>
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<p>Diagram illustrating the definition of fractional change in resistance (FCR) and fractional change in capacitance (FCC) based on biphasic measurements.</p>
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19 pages, 19897 KiB  
Article
A Novel Rainfall Classification for Mapping Rainwater Harvesting: A Case Study in Kalar, Iraq
by Kawa Z. Abdulrahman, Shvan F. Aziz and Moses Karakouzian
Water 2024, 16(22), 3311; https://doi.org/10.3390/w16223311 - 18 Nov 2024
Viewed by 240
Abstract
Increasing water demand driven by population growth and climate change strains water resources, especially in arid regions. The effectiveness of rainwater harvesting (RWH) as a viable solution is contingent upon the meticulous selection of appropriate sites. Contemporary efforts have increasingly utilized Geographic Information [...] Read more.
Increasing water demand driven by population growth and climate change strains water resources, especially in arid regions. The effectiveness of rainwater harvesting (RWH) as a viable solution is contingent upon the meticulous selection of appropriate sites. Contemporary efforts have increasingly utilized Geographic Information Systems (GIS) and remote sensing technologies to optimize the identification of ideal locations for implementing RWH infrastructure. However, inconsistencies in rainfall classification methodologies can compromise the accuracy of the resulted suitability maps. Consequently, a standardized approach to grading rainfall depth for mapping RWH sites becomes imperative. This study presents an innovative rainfall classification method tailored for both micro and macro catchment areas, offering a reliable and adaptable approach to rainfall analysis. By refining classification criteria, this method aims to improve the consistency and precision of RWH mapping, addressing a gap in existing methodologies and providing a more standardized approach. Through the application of FAHP and Fuzzy overlay techniques in ArcGIS 10.4, the study compares traditional rainfall classification with the proposed new classification method to assess RWH suitability in Kalar. The comparison highlights that the new rainfall classification-based map yielded higher accuracy and realism compared to traditional methods. Full article
(This article belongs to the Special Issue Hydroclimate Extremes: Causes, Impacts, and Mitigation Plans)
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<p>Study area.</p>
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<p>Rainfall depths at Kifre, Kalar, and Darbandikhan Stations (DMSS).</p>
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<p>Work flow of the study.</p>
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<p>Rainfall classification map using the traditional classification.</p>
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<p>Rainfall classification map for macro catchments using the new classification.</p>
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<p>Rainfall classification map for micro catchments using the new classification.</p>
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<p>Slope classification map for RWH.</p>
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<p>LULC classification for RWH.</p>
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<p>Map showing the classified soil in the study area.</p>
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<p>Map showing the drainage density classification.</p>
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<p>Maps of (<b>a</b>) rainfall membership, (<b>b</b>) slope membership, (<b>c</b>) soil membership, (<b>d</b>) LULC membership, and (<b>e</b>) drainage density membership.</p>
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<p>Maps of (<b>a</b>) rainfall membership, (<b>b</b>) slope membership, (<b>c</b>) soil membership, (<b>d</b>) LULC membership, and (<b>e</b>) drainage density membership.</p>
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<p>Maps of (<b>a</b>) rainfall membership, (<b>b</b>) slope membership, (<b>c</b>) soil membership, (<b>d</b>) LULC membership, and (<b>e</b>) drainage density membership.</p>
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<p>The final suitability map for RWH using the traditional rainfall classification.</p>
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<p>The final suitability map for macro catchments using the new rainfall classification.</p>
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<p>The final suitability map for micro catchments using the new rainfall classification.</p>
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17 pages, 812 KiB  
Review
Metabolic Dysfunctions, Dysregulation of the Autonomic Nervous System, and Echocardiographic Parameters in Borderline Personality Disorder: A Narrative Review
by Paola Bozzatello, Giacomo Marin, Giulio Gabriele, Claudio Brasso, Paola Rocca and Silvio Bellino
Int. J. Mol. Sci. 2024, 25(22), 12286; https://doi.org/10.3390/ijms252212286 - 15 Nov 2024
Viewed by 229
Abstract
Borderline personality disorder (BPD) is a complex psychiatric disorder characterized by an unstable sense of self and identity, emotional dysregulation, impulsivity, and disturbed interpersonal relationships. This narrative review examines the interplay between dysregulation of the autonomic nervous system, metabolic changes, and cardiovascular risk [...] Read more.
Borderline personality disorder (BPD) is a complex psychiatric disorder characterized by an unstable sense of self and identity, emotional dysregulation, impulsivity, and disturbed interpersonal relationships. This narrative review examines the interplay between dysregulation of the autonomic nervous system, metabolic changes, and cardiovascular risk in BPD. Altered heart rate variability (HRV), reflecting the dysregulation of the autonomic nervous system, is associated with some BPD core symptoms, such as emotional instability and impulsivity. Dysregulation of the hypothalamic–pituitary–adrenal (HPA) axis, often stemming from early trauma, contributes to chronic inflammation and elevated allostatic load, which further increases cardiovascular risk. Metabolic dysfunctions in BPD, such as elevated body mass index (BMI), high blood pressure, and inflammatory markers like C-reactive protein (CRP), exacerbate these risks. Speckle-tracking echocardiography, particularly global longitudinal strain (GLS) and biomarkers such as homocysteine and epicardial fat, could be considered early predictors of cardiovascular events in individuals with BPD. Chronic stress, inflammation, and maladaptive stress responses further heighten cardiovascular vulnerability, potentially accelerating biological aging and cognitive decline. A literature search covering the period from 2014 to 2024 on PubMed identified 189 studies on this topic, of which 37 articles were deemed eligible for this review. These included cross-sectional, longitudinal, case–control, randomised controlled trials (RCTs), reviews, and meta-analysis designs, with sample sizes ranging from 14 to 5969 participants. The main limitations were that only one database was searched, the time of publications was limited, non-English manuscripts were excluded, and the quality of each paper was not commented on. This narrative review aims to provide an overview of recent evidence obtained on this topic, pointing out a direction for future research. Full article
(This article belongs to the Collection Feature Papers in Molecular Neurobiology)
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<p>Illustration of the records obtained from PubMed, along with the subsequent screening process for the studies.</p>
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15 pages, 3407 KiB  
Article
Minimalist Design for Multi-Dimensional Pressure-Sensing and Feedback Glove with Variable Perception Communication
by Hao Ling, Jie Li, Chuanxin Guo, Yuntian Wang, Tao Chen and Minglu Zhu
Actuators 2024, 13(11), 454; https://doi.org/10.3390/act13110454 - 13 Nov 2024
Viewed by 239
Abstract
Immersive human–machine interaction relies on comprehensive sensing and feedback systems, which enable transmission of multiple pieces of information. However, the integration of increasing numbers of feedback actuators and sensors causes a severe issue in terms of system complexity. In this work, we propose [...] Read more.
Immersive human–machine interaction relies on comprehensive sensing and feedback systems, which enable transmission of multiple pieces of information. However, the integration of increasing numbers of feedback actuators and sensors causes a severe issue in terms of system complexity. In this work, we propose a pressure-sensing and feedback glove that enables multi-dimensional pressure sensing and feedback with a minimalist design of the functional units. The proposed glove consists of modular strain and pressure sensors based on films of liquid metal microchannels and coin vibrators. Strain sensors located at the finger joints can simultaneously project the bending motion of the individual joint into the virtual space or robotic hand. For subsequent tactile interactions, the design of two symmetrically distributed pressure sensors and vibrators at the fingertips possesses capabilities for multi-directional pressure sensing and feedback by evaluating the relationship of the signal variations between two sensors and tuning the feedback intensities of two vibrators. Consequently, both dynamic and static multi-dimensional pressure communication can be realized, and the vibrational actuation can be monitored by a liquid-metal-based sensor via a triboelectric sensing mechanism. A demonstration of object interaction indicates that the proposed glove can effectively detect dynamic force in varied directions at the fingertip while offering the reconstruction of a similar perception via the haptic feedback function. This device introduces an approach that adopts a minimalist design to achieve a multi-functional system, and it can benefit commercial applications in a more cost-effective way. Full article
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<p>Multi-dimensional pressure-sensing and feedback glove and its intelligent interaction system. Schematic diagram of the glove’s application in enhanced spatial immersive interaction, including (i) the structural diagram of the pressure sensor, (ii) the components of the vibration haptic feedback module, and (iii) the structural diagram of the bending sensor.</p>
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<p>Sensors of the multi-dimensional pressure-sensing and feedback glove. (<b>a</b>) Optical image of the pressure sensor; (<b>b</b>) optical image of the bending sensor; and (<b>c</b>) optical image of the interactive glove and the corresponding components.</p>
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<p>Working mechanism of the pressure sensor and the bending sensor. (<b>a</b>) The (i) schematic diagram of the pressure sensor, (ii) dimensional changes of the liquid metal electrodes in the normal and pressurized states, and (iii) changes in the A-A’ cross-section of the liquid metal electrodes; and (<b>b</b>) the (i) schematic diagram of the bending sensor, (ii) changes in the liquid metal electrodes in the normal and bending states, and (iii) dimensional changes in the bending sensors observed from view B.</p>
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<p>Characterization of the pressure sensor. (<b>a</b>) Schematic of the characterization method; (<b>b</b>) relationship between the sensor’s output signal and the pressure under loading conditions; (<b>c</b>) relationship between the pressure sensor’s output signal and the pressure under loading and unloading conditions; (<b>d</b>) real-time monitoring of the output signal changes during one cycle of pressure increase and decrease; (<b>e</b>) response and recovery times of the sensor; (<b>f</b>) repeatability test over 2000 cycles at 55 kPa; (<b>g</b>) relationship between the driven voltage of a coin vibration and the collected triboelectric voltage signal of the sensor; and (<b>h</b>) real-time triboelectric voltage signal as the driven voltage continues to increase.</p>
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<p>Characterization of the bending sensor. (<b>a</b>) Schematic of the characterization method; (<b>b</b>) relationship between the sensor’s output signal and the strain under tensile conditions; (<b>c</b>) relationship between the bending sensor’s output signal and the pressure under loading and unloading conditions; (<b>d</b>) response and recovery times of the sensor; (<b>e</b>) repeatability test over 2000 cycles at 20% strain; (<b>f</b>) response of the sensor to strain with a given initial torsion angle; and (<b>g</b>) response of the sensor to strain with a given initial curvature.</p>
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<p>Demonstration application of the multi-dimensional pressure-sensing and feedback glove. (<b>a</b>) Schematic of fingertip pressing status including (i) left side contact, (ii) right side contact, (iii) intermediate contact and (iv) rolling from left to right; (<b>b</b>) real-time output signals of the pressure sensor at different pressing angles; (<b>c</b>) feedback from the coin vibrators at different pressing angles with single vibrator running condition marked by grey and both vibrators running condition marked by pale yellow; (<b>d</b>) output signals from the bending sensor measure the stepped bending of the finger at an angle of 10 degrees each time up to 90 degrees; (<b>e</b>) response of the bending sensor under different bending methods; (<b>f</b>) various hand gestures labelled from ① to ⑧ used to test the bending sensor; (<b>g</b>) output signals corresponding to different hand gestures labelled from ② to ⑧; (<b>h</b>) demonstration of grasping a test tube; (<b>i</b>) feedback from coin vibrators during the grasping process; (<b>j</b>) real-time signal output during the grasp; and (<b>k</b>) snapshot of pressure and bending angles before and after grasping.</p>
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15 pages, 4713 KiB  
Article
The Gene Cluster Cj0423Cj0425 Negatively Regulates Biofilm Formation in Campylobacter jejuni
by Zhi Wang, Yuwei Wu, Ming Liu, Ling Chen, Kaishan Xiao, Zhenying Huang, Yibing Zhao, Huixian Wang, Yu Ding, Xiuhua Lin, Jiahui Zeng, Feiting Peng, Jumei Zhang, Juan Wang and Qingping Wu
Int. J. Mol. Sci. 2024, 25(22), 12116; https://doi.org/10.3390/ijms252212116 - 12 Nov 2024
Viewed by 365
Abstract
Abstract: Campylobacter jejuni (C. jejuni) is a zoonotic foodborne pathogen that is widely distributed worldwide. Its optimal growth environment is microaerophilic conditions (5% O2, 10% CO2), but it can spread widely in the atmospheric environment. Biofilms [...] Read more.
Abstract: Campylobacter jejuni (C. jejuni) is a zoonotic foodborne pathogen that is widely distributed worldwide. Its optimal growth environment is microaerophilic conditions (5% O2, 10% CO2), but it can spread widely in the atmospheric environment. Biofilms are thought to play an important role in this process. However, there are currently relatively few research works on the regulatory mechanisms of C. jejuni biofilm formation. In this study, a pan-genome analysis, combined with the analysis of biofilm phenotypic information, revealed that the gene cluster Cj0423Cj0425 is associated with the negative regulation of biofilm formation in C. jejuni. Through gene knockout experiments, it was observed that the Cj0423Cj0425 mutant strain significantly increased biofilm formation and enhanced flagella formation. Furthermore, pull-down assay revealed that Cj0424 interacts with 93 proteins involved in pathways such as fatty acid synthesis and amino acid metabolism, and it also contains the quorum sensing-related gene luxS. This suggests that Cj0423Cj0425 affects fatty acid synthesis and amino acid metabolism, influencing quorum sensing and strain motility, ultimately inhibiting biofilm formation. Full article
(This article belongs to the Section Molecular Biology)
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<p>Distribution of <span class="html-italic">Cj0423</span>–<span class="html-italic">Cj0425</span> in <span class="html-italic">C. jejuni</span> and its relationship with biofilm. (<b>a</b>) Pan-genome analysis of 234 <span class="html-italic">C. jejuni</span> genomes in the NCBI genome database; (<b>b</b>) <span class="html-italic">Cj0423</span>–<span class="html-italic">Cj0425</span> is not present in all <span class="html-italic">C. jejuni</span>; (<b>c</b>) Association analysis between <span class="html-italic">Cj0423</span>–<span class="html-italic">Cj0425</span> and biofilm formation; most of the strong biofilm formation strains do not contain <span class="html-italic">Cj0423</span>–<span class="html-italic">Cj0425</span>; red color indicates the presence of this gene, blue color indicates that the gene is absent, mauve color represents strong biofilm formation ability strain, gray-green color represents weak biofilm formation ability strain; (<b>d</b>) Distribution of <span class="html-italic">Cj0423</span>–<span class="html-italic">Cj0425</span> and biofilm forming ability.</p>
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<p><span class="html-italic">Cj0423</span>–<span class="html-italic">Cj0425</span> negatively regulates biofilm formation. (<b>a</b>) Determining the growth curves of the wild strain and knockout strain by shaking culture under microaerophilic conditions. (<b>b</b>) Scanning Electron Microscope observation of biofilm. (<b>c</b>) Crystal violet method to determine its biofilm formation ability. (<b>d</b>) Observation of biofilm under laser confocal microscope. SYTO-9 is green fluorescence and stains live cells, while PI is red fluorescence and stains dead cells. “ns” means the <span class="html-italic">p</span> value is greater than 0.05; “****” means the <span class="html-italic">p</span> value is less than 0.0001.</p>
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<p><span class="html-italic">Cj0423</span>–<span class="html-italic">Cj0425</span> inhibits the mobility of <span class="html-italic">C. jejuni</span>. (<b>a</b>) Wild-type strain on the left, mutant strain on the right. (<b>b</b>) The diameter of the mobility was measured, and the significance was analyzed using <span class="html-italic">t</span>-test. “***” means the <span class="html-italic">p</span> value is less than 0.0001.</p>
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<p>RT-qPCR verifies the results of pull-down, extracts DNA from biofilms at different times, and verifies related genes such as motility, chemotaxis, and quorum sensing. “ns” means the <span class="html-italic">p</span> value is greater than 0.05; “*” means the <span class="html-italic">p</span> value is less than 0.05; “**” means the <span class="html-italic">p</span> value is less than 0.01; “***” means the <span class="html-italic">p</span> value is less than 0.001; “****” means the <span class="html-italic">p</span> value is less than 0.0001.</p>
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<p>Protein purification and exogenous addition. (<b>a</b>) SDS-PAGE of the protein purification, in which Lane 1 is the 500 mM imidazole eluate. (<b>b</b>–<b>e</b>) The purified protein Cj0424 was added to ∆<span class="html-italic">Cj0423–Cj0425</span> and wild-type strain for culture, and the amount of biofilm formation at different times was measured. “ns” means the <span class="html-italic">p</span> value is greater than 0.05; “*” means the <span class="html-italic">p</span> value is less than 0.05; “**” means the <span class="html-italic">p</span> value is less than 0.01; “***” means the <span class="html-italic">p</span> value is less than 0.001; “****” means the <span class="html-italic">p</span> value is less than 0.0001.</p>
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<p>Pull-down identification of interacting proteins: (<b>a</b>) Experimental flow chart for pull-down, including ① the bait protein and whole bacterial protein, ② interaction between the bait protein and whole bacterial protein, ③ use of a nickel column to remove unbound proteins and elute interacting proteins, ④ electrophoresis identification of protein interaction results, and ⑤ protein identification using mass spectrometry. (<b>b</b>) SDS-PAGE identification of pull-down results, where Lane 1 represents protein Cj0424, Lane 2 is <span class="html-italic">C. jejuni</span> whole bacterial protein, Lanes 3–5 depict the impurity washing process, and Lane 6 represents the eluate containing Cj0424-interacting proteins. (<b>c</b>) Enrichment of protein pathways. (<b>d</b>) Construction of a protein interaction map.</p>
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<p>Cj0424 affects biofilm formation through multiple pathways. Cj0424 can regulate biofilm formation through quorum sensing, chemotaxis, motility, and oxidative stress.</p>
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27 pages, 3503 KiB  
Review
Frequency Selective Surfaces: Design, Analysis, and Applications
by Waseem Afzal, Muhammad Zeeshan Baig, Amir Ebrahimi, Md. Rokunuzzaman Robel, Muhammad Tausif Afzal Rana and Wayne Rowe
Telecom 2024, 5(4), 1102-1128; https://doi.org/10.3390/telecom5040056 - 5 Nov 2024
Viewed by 625
Abstract
This paper aims to provide a general review of the fundamental ideas, varieties, methods, and experimental research of the most advanced frequency selective surfaces available today. Frequency-selective surfaces are periodic structures engineered to work as spatial filters in interaction with electromagnetic (EM) waves [...] Read more.
This paper aims to provide a general review of the fundamental ideas, varieties, methods, and experimental research of the most advanced frequency selective surfaces available today. Frequency-selective surfaces are periodic structures engineered to work as spatial filters in interaction with electromagnetic (EM) waves with different frequencies, polarization, and incident angles in a desired and controlled way. They are usually made of periodic elements with dimensions less than the operational wavelength. The primary issue examined is the need for more efficient, compact, and adaptable electromagnetic filtering solutions. The research method involved a comprehensive review of recent advancements in FSS design, focusing on structural diversity, miniaturization, multiband operations, and the integration of active components for tunability and reconfigurability. Key findings include the development of highly selective miniaturized FSSs, innovative applications on flexible and textile substrates, and the exploration of FSSs for liquid and strain sensing. The conclusions emphasize the significant potential of FSS technology to enhance wireless communication, environmental monitoring, and defense applications. This study provides valuable insights into the design and application of FSSs, aiming to guide future research and development in this dynamic field. Full article
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<p>Wave interaction with FSS characteristics: (<b>a</b>) bandpass, (<b>b</b>) bandstop, (<b>c</b>) absorber, (<b>d</b>) polarization converter [<a href="#B14-telecom-05-00056" class="html-bibr">14</a>].</p>
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<p>Radomes at the Cryptologic Operations Center, Misawa, Japan (photo courtesy of en. Wikipedia).</p>
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<p>Cassini high-gain antenna (HGA) with a four-band FSS [<a href="#B2-telecom-05-00056" class="html-bibr">2</a>].</p>
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<p>An active LC array comprising metallic strips interrupted by gaps in a periodic fashion. The gaps can be loaded with varactor diodes to alter the gap capacitance [<a href="#B5-telecom-05-00056" class="html-bibr">5</a>].</p>
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<p>Classification of FSSs.</p>
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<p>Current distribution at the first and the second resonant modes—(<b>top</b>) shows the fundamental mode (frequency <math display="inline"><semantics> <msub> <mi>f</mi> <mn>0</mn> </msub> </semantics></math>), which is excited for any element shape irrespective of the incidence angle; (<b>bottom</b>) shows the first odd mode at about 2<math display="inline"><semantics> <msub> <mi>f</mi> <mn>0</mn> </msub> </semantics></math>, which may be excited at oblique incidence. The frequency of this mode may change slightly depending on the element shape [<a href="#B1-telecom-05-00056" class="html-bibr">1</a>].</p>
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<p>Typical shapes of FSSs. (<b>a</b>) N-pole or center-connected; (<b>b</b>) Loop type; (<b>c</b>) Solid interiors; (<b>d</b>) Combination of either first three [<a href="#B112-telecom-05-00056" class="html-bibr">112</a>].</p>
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<p>Examples of the third class of FSSs, the plate type elements [<a href="#B111-telecom-05-00056" class="html-bibr">111</a>].</p>
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<p>Frequency selective surface made of Jerusalem cross elements. (<b>a</b>) Jerusalem cross unit cell, (<b>b</b>) Simulated transmission and reflection coefficient [<a href="#B131-telecom-05-00056" class="html-bibr">131</a>].</p>
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<p>(<b>a</b>) Perspective view of 3D FSS; (<b>b</b>) cross-sectional view of FSS (<b>c</b>); side-view (Picture is taken from [<a href="#B142-telecom-05-00056" class="html-bibr">142</a>]).</p>
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<p>The first miniaturized element FSS. (<b>a</b>) 3D view of FSS, (<b>b</b>) the unit cell of the structure. (Picture taken from [<a href="#B112-telecom-05-00056" class="html-bibr">112</a>]).</p>
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<p>(<b>a</b>) Lumped-element circuit model for miniaturized elements, (<b>b</b>) Comparison between EM and circuit model simulations with <math display="inline"><semantics> <mrow> <mi>L</mi> <mo>=</mo> <mn>1.08</mn> </mrow> </semantics></math> nH and <math display="inline"><semantics> <mrow> <mi>c</mi> <mo>=</mo> <mn>0.15</mn> </mrow> </semantics></math> pF (results are adopted from [<a href="#B112-telecom-05-00056" class="html-bibr">112</a>]).</p>
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<p>Scan angle performance of miniaturized-element FSS; (<b>a</b>) TE Polarization; (<b>b</b>) TM Polarization (results are adopted from [<a href="#B112-telecom-05-00056" class="html-bibr">112</a>]).</p>
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15 pages, 2224 KiB  
Article
Chromosomal Type II Toxin–Antitoxin Systems May Enhance Bacterial Fitness of a Hybrid Pathogenic Escherichia coli Strain Under Stress Conditions
by Jessika C. A. Silva, Lazaro M. Marques-Neto, Eneas Carvalho, Alejandra M. G. Del Carpio, Camila Henrique, Luciana C. C. Leite, Thais Mitsunari, Waldir P. Elias, Danielle D. Munhoz and Roxane M. F. Piazza
Toxins 2024, 16(11), 469; https://doi.org/10.3390/toxins16110469 - 1 Nov 2024
Viewed by 694
Abstract
The functions of bacterial plasmid-encoded toxin–antitoxin (TA) systems are unambiguous in the sense of controlling cells that fail to inherit a plasmid copy. However, its role in chromosomal copies is contradictory, including stress-response-promoting fitness and antibiotic treatment survival. A hybrid pathogenic Escherichia coli [...] Read more.
The functions of bacterial plasmid-encoded toxin–antitoxin (TA) systems are unambiguous in the sense of controlling cells that fail to inherit a plasmid copy. However, its role in chromosomal copies is contradictory, including stress-response-promoting fitness and antibiotic treatment survival. A hybrid pathogenic Escherichia coli strain may have the ability to colonize distinct host niches, facing contrasting stress environments. Herein, we determined the influence of multiple environmental stress factors on the bacterial growth dynamic and expression profile of previously described TA systems present in the chromosome of a hybrid atypical enteropathogenic and extraintestinal E. coli strain. Genomic analysis revealed 26 TA loci and the presence of five type II TA systems in the chromosome. Among the tested stress conditions, osmotic and acid stress significantly altered the growth dynamics of the hybrid strain, enhancing the necessary time to reach the stationary phase. Using qPCR analyses, 80% of the studied TA systems were differentially expressed in at least one of the tested conditions, either in the log or in the stationary phase. These data indicate that type II TA systems may contribute to the physiology of pathogenic hybrid strains, enabling their adaptation to different milieus. Full article
(This article belongs to the Special Issue Toxins: 15th Anniversary)
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<p>(<b>A</b>) Experimental design for bacterial growth under stress. The illustration depicts how the experiments were designed for cultivation under stress conditions, the media employed for bacterial growth, and the bacterial cultivation phase in which samples were collected for qPCR analyses. Created with BioRender.com. Count of BA1250 Colony Forming Units (CFUs/mL) under different culture conditions in the (<b>B</b>) logarithmic and (<b>C</b>) stationary phase. Statistical analysis was performed using the non-parametric <span class="html-italic">t</span>-test, compared to bacteria growth in the LB medium. * <span class="html-italic">p</span>-value &lt; 0.02; ** <span class="html-italic">p</span>-value &lt; 0.002.</p>
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<p>Relative expression of toxin–antitoxins in a log growth phase in duplicates of three independent experiments. <span class="html-italic">E. coli</span> BA1250 toxin–antitoxin gene pairs (<span class="html-italic">ccdB</span>/<span class="html-italic">ccdA</span>, <span class="html-italic">yhaV/prlF</span>, <span class="html-italic">mazE/mazF</span>, <span class="html-italic">yoeB/yefM</span>, and <span class="html-italic">pasT/pasI</span>) were evaluated in the log growth phase under nutritional scarcity, oxidative stress, acid shock, osmotic stress, and LB medium condition. Genes were considered up/downregulated when relative average expression was −1 &gt; Log2Fc &gt; 1 in comparison to the LB group. Statistical significance was considered when the <span class="html-italic">p</span> value &lt; 0.05 in 2-way ANOVA test comparing toxin and antitoxin at the same condition. (*) Represent statistical significance with <span class="html-italic">p</span> &lt; 0.05; (**) represent statistical significance with <span class="html-italic">p</span> &lt; 0.01.</p>
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<p>Relative expression of toxin–antitoxins in the stationary growth phase in duplicates of three independent experiments. <span class="html-italic">E. coli</span> BA1250 toxin–antitoxin gene pairs (<span class="html-italic">ccdB/ccdA</span>, <span class="html-italic">yhaV/prlF</span>, <span class="html-italic">mazE/mazF</span>, <span class="html-italic">yoeB/yefM</span>, and <span class="html-italic">pasT/pasI</span>) were evaluated in the stationary growth phase under nutritional scarcity, oxidative stress, acid shock, osmotic stress, and stress-free LB medium condition. Genes were considered up/downregulated when relative average expression was −1 &gt; Log2Fc &gt; 1 in comparison to the LB group. Statistical significance was considered when the <span class="html-italic">p</span> value &lt; 0.05 in a 2-way ANOVA test comparing toxin and antitoxin at the same condition. (*) Represent statistical significance with <span class="html-italic">p</span> &lt; 0.05; (****) represent statistical significance with <span class="html-italic">p</span> &lt; 0.0001.</p>
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<p>(<b>A</b>) Barplot demonstrating the predicted presence and absence of the 39 gene components of TA systems detected into aEPEC, ExPEC, Hybrid, and tEPEC strains chromosomes and plasmids. Bars indicate the percentage of predicted gene presence among the bacterial strains within each pathotype. Gene predicted rate of type I, II, IV, and V TA system components present in aEPEC, ExPEC, Hybrid, and tEPEC strains in the (<b>B</b>) chromosome and in the (<b>C</b>) plasmid. Bars indicate the percentage of predicted gene presence among the bacterial strains within each TA system type. * <span class="html-italic">p</span> &lt; 0.05, as determined by a non-parametric one-way ANOVA test.</p>
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17 pages, 6878 KiB  
Article
Study on Coal Seam Roof Failure Based on Optical Fiber Acoustic Sensing and the Parallel Electrical Method
by Zilong She, Bo Wang, Yan Zhang, Linfeng Zeng, Liujun Xie and Sihongren Shen
Energies 2024, 17(21), 5471; https://doi.org/10.3390/en17215471 - 31 Oct 2024
Viewed by 576
Abstract
As China enters the stage of deep coal mining, the accidents caused by roof failure pose increasingly serious threats. Current research on roof failure zones often use single methods, but single geophysical data may result in multisolution issues during interpretation. This paper employed [...] Read more.
As China enters the stage of deep coal mining, the accidents caused by roof failure pose increasingly serious threats. Current research on roof failure zones often use single methods, but single geophysical data may result in multisolution issues during interpretation. This paper employed similar simulation experiments, exploring the strain failure characteristics and the changes in apparent resistivity caused by stress variations, taking the 11-3106 working face of a mining area as the research object. Through optical fiber strain and apparent resistivity, the locations and degrees of fracture in postmining rock strata were identified. The feasibility of using distributed optical fiber sensing and the parallel electrical method for qualitative and quantitative analysis of mining-induced fractures was verified. The results showed that optical fiber strain increased significantly at the location of rock fracture, with apparent resistivity anomalies rising correspondingly. The peak strain region corresponded well with the region of apparent resistivity anomalies. In a similar simulation with a geometric ratio of 1:100, the height of the caving zone was measured to be 31.65 cm, with a caving-to-mining ratio of 6.33. In the field working face, the caving zone height was 29.47 m, with a caving-to-mining ratio of 6.01, consistent with the actual conditions of the 11-3106 working face. Full article
(This article belongs to the Topic Mining Safety and Sustainability, 2nd Volume)
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<p>Schematic diagram of optical fiber deformation under stress.</p>
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<p>Schematic diagram of parallel electrical method monitoring.</p>
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<p>Monitoring equipment diagram.</p>
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<p>Experimental system deployment program.</p>
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<p>Diagram of roof development process. (<b>a</b>) Noncaving stage; (<b>b</b>) development stage of the caving zone; (<b>c</b>) development stage of the water-flowing fractured zone; (<b>d</b>) stable stage of the water-flowing fractured zone.</p>
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<p>Periodic collapse of rock strata. (<b>a</b>) Rockfall; (<b>b</b>) New fracture appear; (<b>c</b>) Fissure enlargement; (<b>d</b>) Fissure closure.</p>
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<p>Diagram of model background.</p>
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<p>Diagram of experimental results. (<b>a</b>) Excavation 30 cm; (<b>b</b>) Excavation 60 cm; (<b>c</b>) Excavation 80 cm; (<b>d</b>) Excavation 90 cm; (<b>e</b>) Excavation 120 cm; (<b>f</b>) Excavation 150 cm; (<b>g</b>) Excavation 180 cm; (<b>h</b>) Excavation 200 cm.</p>
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<p>Diagram of experimental results. (<b>a</b>) Excavation 30 cm; (<b>b</b>) Excavation 60 cm; (<b>c</b>) Excavation 80 cm; (<b>d</b>) Excavation 90 cm; (<b>e</b>) Excavation 120 cm; (<b>f</b>) Excavation 150 cm; (<b>g</b>) Excavation 180 cm; (<b>h</b>) Excavation 200 cm.</p>
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<p>Schematic diagram of the monitoring system.</p>
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<p>Diagram of on-site monitoring results. (<b>a1</b>) Parallel electrical method−drill port 100 m; (<b>a2</b>) Optical fiber acoustic sensing−drill port 100 m; (<b>b1</b>) Parallel electrical method−drill port 80 m; (<b>b2</b>) Optical fiber acoustic sensing−drill port 80 m; (<b>c1</b>) Parallel electrical method−drill port 60 m; (<b>c2</b>) Optical fiber acoustic sensing−drill port 60 m; (<b>d1</b>) Parallel electrical method−drill port 40 m; (<b>d2</b>) Optical fiber acoustic sensing−drill port 40 m; (<b>e1</b>) Parallel electrical method−drill port 20 m; (<b>e2</b>) Optical fiber acoustic sensing−drill port 20 m.</p>
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<p>Diagram of on-site monitoring results. (<b>a1</b>) Parallel electrical method−drill port 100 m; (<b>a2</b>) Optical fiber acoustic sensing−drill port 100 m; (<b>b1</b>) Parallel electrical method−drill port 80 m; (<b>b2</b>) Optical fiber acoustic sensing−drill port 80 m; (<b>c1</b>) Parallel electrical method−drill port 60 m; (<b>c2</b>) Optical fiber acoustic sensing−drill port 60 m; (<b>d1</b>) Parallel electrical method−drill port 40 m; (<b>d2</b>) Optical fiber acoustic sensing−drill port 40 m; (<b>e1</b>) Parallel electrical method−drill port 20 m; (<b>e2</b>) Optical fiber acoustic sensing−drill port 20 m.</p>
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15 pages, 5629 KiB  
Article
FBG and BOTDA Based Monitoring of Mine Pressure Under Remaining Coal Pillars Using Physical Modeling
by Dingding Zhang, Zhi Li, Yanyan Duan, Long Yang and Hongrui Liu
Sensors 2024, 24(21), 7037; https://doi.org/10.3390/s24217037 - 31 Oct 2024
Viewed by 381
Abstract
Strong mine pressure often emerges when the working face of the lower coal seam in a closely spaced coal seam system passes through the remaining coal pillar in the overlying goaf. This study investigates the law of overburden movement and the manifestation of [...] Read more.
Strong mine pressure often emerges when the working face of the lower coal seam in a closely spaced coal seam system passes through the remaining coal pillar in the overlying goaf. This study investigates the law of overburden movement and the manifestation of mine pressure during mining under the remaining coal pillar. A physical model measuring 2.5 × 0.2 × 1.503 m is constructed. Fiber Bragg grating sensing technology (FBG) and Brillouin optical time domain analysis technology (BOTDA) are employed in the physical model experiment to monitor the internal strain of the overlying rock as the working face advances. This study determines the laws of overlying rock fracture and working face pressure while mining coal seams beneath the remaining coal pillar. It analyzes the relationship between the pressure at the working face and the strain characteristics of the horizontally distributed optical fiber. A fiber grating characterization method is established for the stress evolution law of overlying rock while passing the remaining coal pillar. The experimental results indicated that the fracture angle of overlying rock gradually decreases during the mining stage through and after the coal pillar. In the mining stage through the coal pillar, the cycle pressure step distance of the working face is reduced by 33.3% compared to the stage after mining through the coal pillar. Initially, the strain pattern of the horizontal optical fiber is unimodal when pressure is first applied to the working face, and it transitions from unimodal to bimodal during periodic pressure. The peak value of fiber Bragg grating compressive strain and the range of influence of advanced support pressure are 3.6 times and 4.8 times, respectively, before passing through the remaining coal pillar. Finally, the accuracy of the FBG characterization method is verified by comparing it to the monitoring curve of the coal seam floor pressure sensor. The research results contribute to applying fiber optic sensing technology in mining physical model experiments. Full article
(This article belongs to the Special Issue Optical Sensors for Industrial Applications)
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<p>Monitoring principle of BOTDA.</p>
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<p>Physical model lithology distribution and sensor layout.</p>
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<p>The collapse form of overlying rock after 1<sup>−2</sup> coal seam mining.</p>
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<p>Characteristics of overlying rock collapse as the working face advances.</p>
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<p>DFOS strain curve before mining through coal pillar. (<b>a</b>) Advance from 500 to 1000 mm; (<b>b</b>) Overlying rock collapse characteristics when advancing 850 mm.</p>
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<p>DFOS strain curve before mining through coal pillar. (<b>a</b>) Advance from 110 to 1250 mm; (<b>b</b>) Overlying rock collapse characteristics when advancing 1250 mm.</p>
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<p>DFOS strain curve after mining through coal pillar. (<b>a</b>) Advance from 1350 to 2300 mm; (<b>b</b>) Overlying rock collapse characteristics when advancing 1450 mm.</p>
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<p>FBG strain changes during the process of advancing the working face.</p>
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<p>FBG test results and their corresponding relationship with the position of the remaining coal pillar.</p>
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<p>Comparison of monitoring curves between FBG and CFP as the working face advances.</p>
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10 pages, 3759 KiB  
Communication
From Fiber Layout to the Sensor: Preparation Methods as Key Factors for High-Quality Coupled-Core-Fiber Sensors
by F. Lindner, J. Bierlich, M. Alonso-Murias, D. Maldonado-Hurtado, J. A. Flores-Bravo, S. Sales, J. Villatoro and K. Wondraczek
Sensors 2024, 24(21), 6999; https://doi.org/10.3390/s24216999 - 30 Oct 2024
Viewed by 491
Abstract
During recent years, the optical-fiber-based simultaneous sensing of strain and temperature has attracted increased interest for different applications, e.g., in medicine, architecture, and aerospace. Specialized fiber layouts further enlarge the field of applications at much lower costs and with easier handling. Today, the [...] Read more.
During recent years, the optical-fiber-based simultaneous sensing of strain and temperature has attracted increased interest for different applications, e.g., in medicine, architecture, and aerospace. Specialized fiber layouts further enlarge the field of applications at much lower costs and with easier handling. Today, the performance of many sensors fabricated from conventional fibers suffers from cross-sensitivity (temperature and strain) and relatively high interrogation costs. In contrast, customized fiber architectures would make it possible to circumvent such sensor drawbacks. Here, we report on the development of a high-quality coupled-core fiber and its performance for sensors—from the initial fiber layout via elaboration of the preform and fiber up to the sensor evaluation. A compact, high-speed, and cost-effective interrogation unit using such a specialized coupled-core fiber has been designed to monitor reflectivity changes while even being able to distinguish the direction of the force or impact. Several fiber core material techniques and approaches were investigated, which made it possible to obtain a sufficient volume of material for the required fiber core number and a specialized fiber core geometry in terms of core distances and radial refractive index profile, whilst handling the non-symmetrical fiber architectures of such modeled, complex structures and balancing resources and efforts. Full article
(This article belongs to the Special Issue Advanced Optics and Photonics Technologies for Sensing Applications)
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<p>Sketch of the coupled core fiber.</p>
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<p>Concept of the coupled-core fiber sensor fabrication steps.</p>
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<p>Radial refractive index profiles of (<b>a</b>) the GeO<sub>2</sub>-doped MCVD core preform and (<b>b</b>) an exemplary REPUSIL preform.</p>
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<p>Front views of two-coupled-core fiber preforms using the MCVD method to fabricate GeO<sub>2</sub> doped core rods (marked in red in the photographs). (<b>a</b>) Hexagonal stacking of SiO<sub>2</sub> cladding rods and GeO<sub>2</sub>-doped core rods; and (<b>b</b>) drilled preform with two core rods.</p>
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<p>Refractive index profile of the fabricated Ge-doped two-coupled-core fiber (TCF). The inset photograph shows the cross-section of the fabricated fiber.</p>
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<p>Schematics of the sensor architecture. SMF is single-mode fiber, TCF is two-coupled-core fiber, and <span class="html-italic">L</span> is the length of the TCF segment.</p>
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<p>FBG sensor fabrication. (<b>a</b>) Micrograph of the splicing point between the standard single-mode fiber and the two-core fiber. (<b>b</b>) Micrograph of the femtosecond point-by-point FBG inscription in the off-center core. (<b>c</b>) FBG spectrum (blue: reflected optical power, red: transmitted optical power). The black ellipses indicate the areas of interest (<b>a</b>,<b>b</b>).</p>
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<p>Illustration of the bending effect on the two-coupled-core fiber with FBGs in both cores. The blue color indicates that the light in this core is weaker than in the other core of the TCF. The arrows indicate the direction of bending.</p>
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<p>Experimental results of nearly isothermal bending on a TCF with Bragg grating. The direction of bending with respect to the core orientation of the TCF is indicated with the arrow. (<b>a</b>) Downward bending, (<b>b</b>) upward bending.</p>
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12 pages, 2548 KiB  
Article
In Situ Enrichment of Anammox Bacteria from Pig Farm Anoxic Sludge Through Co-Cultivation with a Quorum-Sensing Functional Strain Pseudomonas aeruginosa
by Yong Liu, Yiru Zhu, Jianping Deng, Bing Yan, Jian Zhan, Yuansong Wei, Hanbing Nie and Shuanglin Gui
Fermentation 2024, 10(11), 548; https://doi.org/10.3390/fermentation10110548 - 25 Oct 2024
Viewed by 553
Abstract
Anaerobic ammonium oxidation (anammox), as an efficient and low-carbon method for nitrogen removal from wastewater, faces the challenge of slow enrichment of functional bacteria. In this study, the enrichment of anammox bacteria Candidatus Brocadia was successfully accelerated by co-culturing with the quorum-sensing strain [...] Read more.
Anaerobic ammonium oxidation (anammox), as an efficient and low-carbon method for nitrogen removal from wastewater, faces the challenge of slow enrichment of functional bacteria. In this study, the enrichment of anammox bacteria Candidatus Brocadia was successfully accelerated by co-culturing with the quorum-sensing strain Pseudomonas aeruginosa and anoxic sludge from a pig farm. Experimental results showed that the R2, which had Pseudomonas aeruginosa added, exhibited chemical reaction ratios RS (NO2-N consumption/NH4+-N consumption) and RP (NO3-N production/NH4+-N consumption) closer to the theoretical values of the anammox reaction since Phase Ⅱ. Bacterial community analysis indicated that the abundance of Candidatus Brocadia in R2 reached 1.63% in cycle 20, significantly higher than the 0.45% in R1. More quorum-sensing signaling molecules, primarily C6-HSL, were detected in R2. C6-HSL was positively correlated with processes such as the secretion of anammox extracellular polymers (EPS) and the regulation of nitric oxide reductase (Nir), which may explain the reason behind the accelerated increase in the abundance of Candidatus Brocadia through co-culturing. Moreover, the metabolism of the dominant genus Paracoccus within the two groups of reactors also showed positive regulation by C6-HSL, with its abundance trend similar to that of Candidatus Brocadia, jointly completing the nitrogen removal process in the reactors. However, it is still unknown which genera secrete large amounts of C6-HSL after inoculation with Pseudomonas aeruginosa. This research provides a novel and low-cost method for the enrichment of anammox bacteria. Full article
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<p>Monitoring of nitrogen concentrations in different forms during long-term synthetic wastewater treatment by anammox in situ enrichment reactors. (<b>a</b>) R1. (<b>b</b>) R2.</p>
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<p>Nitrogen removal rate of anammox in situ enrichment reactors during long-term synthetic wastewater treatment. (<b>a</b>) R1. (<b>b</b>) R2.</p>
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<p>The reaction ratios R<sub>S</sub> (NO<sub>2</sub><sup>−</sup>-N consumption/NH<sub>4</sub><sup>+</sup>-N consumption) and R<sub>P</sub> (NO<sub>3</sub><sup>−</sup>-N production/NH<sub>4</sub><sup>+</sup>-N consumption) of the anammox in situ enrichment reactors. (<b>a</b>) R1. (<b>b</b>) R2.</p>
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<p>Bacterial community analysis of anammox in situ enrichment reactors in cycles 20 and 64.</p>
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<p>Secretion of quorum-sensing signal molecule C6-HSL and C8-HSL in anammox in situ enrichment reactors. Error bars represent standard deviations of triplicate measurements.</p>
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14 pages, 4241 KiB  
Article
Comparison of Aroma and Taste Profiles Between Two Fermented Pea Pastes Using Intelligent Sensory Analysis and Gas Chromatography–Mass Spectrometry
by Tianyang Wang, Lian Yang, Wanting Tang, Haibin Yuan, Chuantao Zeng, Ping Dong, Yuwen Yi, Jing Deng, Huachang Wu and Ju Guan
Fermentation 2024, 10(11), 543; https://doi.org/10.3390/fermentation10110543 - 24 Oct 2024
Viewed by 486
Abstract
The traditionally produced pea paste (PP) suffers from suboptimal flavor and inferior quality. Based on the study of single-strain fermentation, we further selected S. cerevisiae, Z. rouxii, and L. paracasei for PP production by dual-strain fermentation (SL, ZL). By combining intelligent [...] Read more.
The traditionally produced pea paste (PP) suffers from suboptimal flavor and inferior quality. Based on the study of single-strain fermentation, we further selected S. cerevisiae, Z. rouxii, and L. paracasei for PP production by dual-strain fermentation (SL, ZL). By combining intelligent sensory technology, gas chromatography–mass spectrometry (GC-MS), and ultra-high-performance liquid chromatography (UPLC) technology, the aroma and taste characteristics of SL- and ZL-fermented PP were compared. The electronic nose and tongue revealed the differences in the aroma and taste characteristics between the two fermentation methods for fermenting PP. In total, 74 volatile compounds (VOCs) in PP were identified through GC-MS analysis. In contrast, the number of VOCs and the concentrations of alcohols and acids compounds in SL were higher than in ZL. Among the 15 VOCs that were common to both and had significant differences, the concentrations of ethanol, 1-pentanol, and ethyl acetate were higher in SL. For taste characteristics, SL demonstrated significantly higher levels of sweet and bitter amino acids, as well as tartaric acid, compared with ZL. These results elucidate the flavor differences of dual-strain fermented PP, providing a theoretical basis for selecting suitable strains for fermenting PP. Full article
(This article belongs to the Special Issue Analysis of Quality and Sensory Characteristics of Fermented Products)
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<p>The rPCA model based on the response values of the intelligent sensory E-nose and E-tongue sensors. (<b>a</b>,<b>c</b>) Score chart of the model’s load, (<b>b</b>,<b>d</b>) Pearson correlation coefficients of the sensors’ response value and its importance to PC 1.</p>
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<p>The types and relative contents of volatile organic compounds in SL and ZL, represented as (<b>a</b>) a pie chart and (<b>b</b>) a bar chart. A, B, C, D, E, F, and G in the figure represent alcohols, aldehydes, acids, esters, ketones, heterocycles, and others, respectively. The number after the letter indicates the quantity of the compound. *** indicates a significant difference according to Tukey’s HSD and a post facto test (<span class="html-italic">p</span> &lt; 0.05).</p>
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<p>The rPCA model established on basis of the concentration of compounds with significant differences found by GC-MS. (<b>a</b>) Score map of model loading; (<b>b</b>) Pearson correlation coefficients between the concentration of each compound and its importance on PC 1.</p>
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<p>The rPCA model was established on the basis of the concentration of compounds with significant differences found via the free amino acid content. (<b>a</b>) Score map of model loading; (<b>b</b>) Pearson correlation coefficients between the concentration of each compound and its importance on PC 1, (<b>c</b>) Histogram of the taste properties of free amino acids. *** indicates a significant difference by Tukey’s HSD and a post facto test (<span class="html-italic">p</span> &lt; 0.05).</p>
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<p>The rPCA established from the content of six organic acids detected in PP. (<b>a</b>) Score map of model loading; (<b>b</b>) Pearson correlation coefficients between the concentration of each compound and its importance on PC 1. (<b>c</b>) Bar chart of the classification of six organic acids.* and *** denote statistically significant and highly significant differences, respectively.</p>
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<p>Spearman’s correlation heatmap, showing correlations between compounds and the sensors’ responses. (<b>a</b>) Correlation between volatile compound levels and the electronic nose sensors’ responses. (<b>b</b>) Correlation between organic acids/free amino acids and the electronic tongue sensors’ reactions. Red represents positive correlations, and blue represents negative correlations. The symbols “*” and “**” represent significance at <span class="html-italic">p</span> &lt; 0.05 and <span class="html-italic">p</span> &lt; 0.01, respectively.</p>
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9 pages, 197 KiB  
Article
Eight Conditions That Will Change Mining Work in Mining 4.0
by Joel Lööw and Jan Johansson
Mining 2024, 4(4), 904-912; https://doi.org/10.3390/mining4040050 - 24 Oct 2024
Viewed by 823
Abstract
The mining industry is undergoing a transformation driven by the adoption of Industry 4.0 technologies, implementing autonomous trucks, drones, positions systems, and similar technologies. This article, drawing on experiences and observations from several studies conducted in the mining industry, explores the impact of [...] Read more.
The mining industry is undergoing a transformation driven by the adoption of Industry 4.0 technologies, implementing autonomous trucks, drones, positions systems, and similar technologies. This article, drawing on experiences and observations from several studies conducted in the mining industry, explores the impact of these technologies on mining work. It identifies eight key potential changes in working conditions. Firstly, routine and dangerous tasks are increasingly automated, reducing physical strain but potentially leading to job displacement and increased maintenance demands. Secondly, operators and managers are shifting toward handling disturbances and training algorithms, as AI takes over decision-making processes. Thirdly, managers are responsible for more capital with fewer people, potentially altering managerial roles and spans of control. Fourthly, the global connectivity of operations makes the world both larger and smaller, with a universal language blurring boundaries. Fifthly, work becomes location-independent, allowing for remote operation and management. Sixthly, the distinction between work and private life blurs, with increased availability expected from operators and managers. Seventhly, technology expands human senses, providing real-time data and situational awareness. Eighthly and lastly, the pervasive collection and retention of data create a scenario where one’s history is inescapable, raising concerns about data ownership and privacy. These changes necessitate a strategic response from the mining industry to ensure socially sustainable technology development and to attract a future workforce. Full article
(This article belongs to the Special Issue Envisioning the Future of Mining, 2nd Edition)
9 pages, 1515 KiB  
Article
Temperature and Lateral Pressure Sensing Using a Sagnac Sensor Based on Cascaded Tilted Grating and Polarization-Maintaining Fibers
by Yifan Liu, Yujian Li, Pin Xu and Changyuan Yu
Sensors 2024, 24(21), 6779; https://doi.org/10.3390/s24216779 - 22 Oct 2024
Viewed by 404
Abstract
This study introduces a Sagnac Interferometer (SI) fiber sensor that integrates Polarization-Maintaining Fibers (PMFs) with a Tilted Fiber Bragg Grating (TFBG) for the dual-parameter measurement of strain and lateral pressure. By incorporating a 6° TFBG with PMFs into the SI sensor, its sensitivity [...] Read more.
This study introduces a Sagnac Interferometer (SI) fiber sensor that integrates Polarization-Maintaining Fibers (PMFs) with a Tilted Fiber Bragg Grating (TFBG) for the dual-parameter measurement of strain and lateral pressure. By incorporating a 6° TFBG with PMFs into the SI sensor, its sensitivity is significantly enhanced, enabling advanced multi-parameter sensing capabilities. The sensor demonstrates a temperature sensitivity of −1.413 nm/°C and a lateral pressure sensitivity of −4.264 dB/kPa, as validated by repeated experiments. The results exhibit excellent repeatability and high precision, underscoring the sensor’s potential for robust and accurate multi-parameter sensing applications. Full article
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Figure 1

Figure 1
<p>(<b>a</b>) Structure of the core-to-core linking of TFBG and PMF. (<b>b</b>) Structure of the designed sensor.</p>
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<p>Transmission spectrum of SI combined with TFBG.</p>
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<p>Temperature measurement experiment.</p>
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<p>(<b>a</b>) Obtained transmission spectrum, (<b>b</b>) spectrum at 47 °C, (<b>c</b>) detailed graph of peak 1, and (<b>d</b>) linear fit of temperature sensing. (<b>e</b>) Intensity change of Peak A with temperature variation).</p>
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<p>Lateral pressure measurement experiment.</p>
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<p>(<b>a</b>) Obtained transmission spectrum, (<b>b</b>) detailed graph of peak at 47 °C, and (<b>c</b>) linear fit of temperature sensing.</p>
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