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Search Results (3,458)

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Keywords = spatial-temporal dynamic

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24 pages, 8231 KiB  
Article
Adaptive Optimization and Dynamic Representation Method for Asynchronous Data Based on Regional Correlation Degree
by Sichao Tang, Yuchen Zhao, Hengyi Lv, Ming Sun, Yang Feng and Zeshu Zhang
Sensors 2024, 24(23), 7430; https://doi.org/10.3390/s24237430 (registering DOI) - 21 Nov 2024
Abstract
Event cameras, as bio-inspired visual sensors, offer significant advantages in their high dynamic range and high temporal resolution for visual tasks. These capabilities enable efficient and reliable motion estimation even in the most complex scenes. However, these advantages come with certain trade-offs. For [...] Read more.
Event cameras, as bio-inspired visual sensors, offer significant advantages in their high dynamic range and high temporal resolution for visual tasks. These capabilities enable efficient and reliable motion estimation even in the most complex scenes. However, these advantages come with certain trade-offs. For instance, current event-based vision sensors have low spatial resolution, and the process of event representation can result in varying degrees of data redundancy and incompleteness. Additionally, due to the inherent characteristics of event stream data, they cannot be utilized directly; pre-processing steps such as slicing and frame compression are required. Currently, various pre-processing algorithms exist for slicing and compressing event streams. However, these methods fall short when dealing with multiple subjects moving at different and varying speeds within the event stream, potentially exacerbating the inherent deficiencies of the event information flow. To address this longstanding issue, we propose a novel and efficient Asynchronous Spike Dynamic Metric and Slicing algorithm (ASDMS). ASDMS adaptively segments the event stream into fragments of varying lengths based on the spatiotemporal structure and polarity attributes of the events. Moreover, we introduce a new Adaptive Spatiotemporal Subject Surface Compensation algorithm (ASSSC). ASSSC compensates for missing motion information in the event stream and removes redundant information, thereby achieving better performance and effectiveness in event stream segmentation compared to existing event representation algorithms. Additionally, after compressing the processed results into frame images, the imaging quality is significantly improved. Finally, we propose a new evaluation metric, the Actual Performance Efficiency Discrepancy (APED), which combines actual distortion rate and event information entropy to quantify and compare the effectiveness of our method against other existing event representation methods. The final experimental results demonstrate that our event representation method outperforms existing approaches and addresses the shortcomings of current methods in handling event streams with multiple entities moving at varying speeds simultaneously. Full article
(This article belongs to the Section Optical Sensors)
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Figure 1
<p>Schematic diagram of the human retina model and corresponding event camera pixel circuit.</p>
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<p>(<b>a</b>) We consider the light intensity change signals received by the corresponding pixels as computational elements in the time domain. (<b>b</b>) From the statistical results, it can be seen that the ON polarity ratio varies randomly over the time index.</p>
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<p>This graph represents the time span changes of each event cuboid processed by our algorithm.</p>
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<p>This figure illustrates the time surface of events in the original event stream. For clarity, only the x–t components are shown. Red crosses represent non-main events, and blue dots represent main events. (<b>a</b>) In the time surface described in [<a href="#B50-sensors-24-07430" class="html-bibr">50</a>] (corresponding to Formula (24)), only the occurrence frequency of the nearest events around the main event is considered. Consequently, non-main events with disruptive effects may have significant weight. (<b>b</b>) The local memory time surface corresponding to Formula (26) considers the influence weight of historical events within the current spatiotemporal window. This approach reduces the ratio of non-main events involved in the time surface calculation, better capturing the true dynamics of the event stream. (<b>c</b>) By spatially averaging the time surfaces of all events in adjacent cells, the time surface corresponding to Formula (29) can be further regularized. Due to the spatiotemporal regularization, the influence of non-main events is almost completely suppressed.</p>
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<p>Schematic of the Gromov–Wasserstein Event Discrepancy between the original event stream and the event representation results.</p>
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<p>Illustration of the grid positions corresponding to non-zero entropy values.</p>
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<p>Grayscale images and 3D event stream diagrams for three captured scenarios: (<b>a</b>) Grayscale illustration of the corresponding scenarios; (<b>b</b>) 3D event stream illustration of the corresponding scenarios.</p>
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<p>Grayscale images and 3D event stream diagrams for three captured scenarios: (<b>a</b>) Grayscale illustration of the corresponding scenarios; (<b>b</b>) 3D event stream illustration of the corresponding scenarios.</p>
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<p>The variation of the value of <math display="inline"><semantics> <mrow> <msub> <mrow> <mi>GWED</mi> </mrow> <mi mathvariant="normal">N</mi> </msub> </mrow> </semantics></math> corresponding to each algorithm with different numbers of event samples.</p>
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<p>Illustration of the event stream processing results for Scene A by different algorithms: (<b>a</b>) TORE; (<b>b</b>) ATSLTD; (<b>c</b>) Voxel Grid; (<b>d</b>) MDES; (<b>e</b>) Ours.</p>
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<p>APED data obtained from the event stream processing results for Scene A by different algorithms.</p>
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<p>Illustration of the event stream processing results for Scene B by different algorithms: (<b>a</b>) TORE; (<b>b</b>) ATSLTD; (<b>c</b>) Voxel Grid; (<b>d</b>) MDES; (<b>e</b>) Ours.</p>
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<p>APED data obtained from the event stream processing results for Scene B by different algorithms.</p>
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<p>Illustration of the event stream processing results for Scene C by different algorithms: (<b>a</b>) TORE; (<b>b</b>) ATSLTD; (<b>c</b>) Voxel Grid; (<b>d</b>) MDES; (<b>e</b>) Ours.</p>
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<p>APED data obtained from the event stream processing results for Scene C by different algorithms.</p>
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26 pages, 5841 KiB  
Article
Sensitization and Habituation of Hyper-Excitation to Constant Presentation of Pattern-Glare Stimuli
by Thomas Jefferis, Cihan Dogan, Claire E. Miller, Maria Karathanou, Austyn Tempesta, Andrew J. Schofield and Howard Bowman
Neurol. Int. 2024, 16(6), 1585-1610; https://doi.org/10.3390/neurolint16060116 (registering DOI) - 21 Nov 2024
Viewed by 124
Abstract
Background/Objectives: Pattern glare, associated with cortical hyperexcitability, induces visual distortions and discomfort, particularly in individuals susceptible to migraines or epilepsy. While previous research has primarily focused on transient EEG responses to patterned stimuli, this study aims to investigate how continuous presentation of pattern-glare [...] Read more.
Background/Objectives: Pattern glare, associated with cortical hyperexcitability, induces visual distortions and discomfort, particularly in individuals susceptible to migraines or epilepsy. While previous research has primarily focused on transient EEG responses to patterned stimuli, this study aims to investigate how continuous presentation of pattern-glare stimuli affects neural adaptation over both fine (seconds) and coarse (entire experiment) temporal scales. Methods: EEG recordings were obtained from 40 healthy participants exposed to horizontal square-wave gratings at three spatial frequencies presented continuously for three seconds each across multiple trials. Participants’ susceptibility to visual stress, headaches, and discomfort was assessed using questionnaires before and during the experiment. The experiment employed a two-by-two design to evaluate habituation (exponentially decreasing response) and sensitisation (exponentially increasing response) effects at two different time granularities. Mass univariate analysis with cluster-based permutation tests was conducted to identify significant brain response changes during the period of constant stimulation, which we call the DC-shift period. Results: Significant effects were observed during the DC-shift period, indicating sustained hyper-excitation to the medium-pattern glare stimulus. In particular, the mean/intercept analysis revealed a consistent positive-going response to the medium stimulus throughout the DC-shift period, suggesting continued neural engagement. Participants reporting higher discomfort exhibited sensitisation at fine temporal granularity and habituation at coarser temporal granularity. These effects were predominantly localised to the right posterior scalp regions. Conclusions: The study demonstrates that individuals sensitive to pattern-glare stimuli exhibit dynamic neural adaptation characterised by short-term sensitisation and long-term habituation. These findings enhance the understanding of cortical hyperexcitability mechanisms and may inform future interventions for visual-stress-related conditions, such as migraines and epilepsy. Further research is needed to explore the underlying neural processes and validate these effects in clinical populations. Full article
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Figure 1
<p>Illustration of the three stimuli used in the pattern glare test and our experiment. (<b>a</b>) First control pattern, 0.5 c/deg (thick); (<b>b</b>) clinically relevant pattern, 3 c/deg (medium); and (<b>c</b>) third control pattern, 12 c/deg (thin). Here the stripes have been scaled so as to avoid distortions in print but are representative of the stimuli shown to the participants.</p>
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<p>Schematic representation of a single trial. This sequence was repeated 6 times per stimulus type (Thick, Medium or Thin) to complete one block (partition) of experiments.</p>
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<p>Intercept regressor for the average of onsets 2–8. X axis represents participants, Y axis represents the scores entered in the regressor for each participant. The full set of participants are duplicated on the x-axis. This is because Fieldtrip implements a one-sample <span class="html-italic">t</span>-test by performing a 2-sample <span class="html-italic">t</span>-test on a duplicated set of participants—see text in brackets beginning ‘Due to the way Fieldtrip’ in previous paragraph.</p>
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<p>Discomfort regressor for the average of onsets 2–8. X axis represents participants, Y axis represents the scores entered in the regressor for each participant. Thus, a column vector is constructed from the numbers in this panel, which becomes a regressor entered into the regression model. For example, a sample (time-space point) in the EEG data that shows a high response for participants with high values on this regressor and a low response for those low on this regressor will obtain a high coefficient for this regressor, indicating a strong correlation between discomfort score and brain response.</p>
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<p>(<b>A</b>) Left: regressor for discomfort by decrease across the blocks/partitions before orthogonalization. X axis represents participants, Y axis represents the scores of each participant. Right: regressor for discomfort by increase across the blocks/partitions before orthogonalization. X axis represents participants, Y axis represents the scores of each participant. (<b>B</b>) Left: regressor for discomfort by decrease across the blocks/partitions after orthogonalization. X axis represents participants, Y axis represents the scores of each participant. Right: regressor for discomfort by increase across the blocks/partitions after orthogonalization. X axis represents participants, Y axis represents the scores of each participant.</p>
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<p>Discomfort by increase in the onsets by decrease in the partitions, orthogonalized regressor. X axis represents participants in each partition with dashed vertical line to mark partition boundaries, Y axis represents the scores of each participant, Onset-pair, Partition that is entered into the regressor.</p>
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<p>Mean/intercept onsets 2–8 positive cluster in the DC-shift period. (<b>a</b>) Topographic maps through time for the whole period, with red crosses indicating the significant cluster, the colour bar on the right represents T-statistic on the topographic maps. (<b>b</b>) Grand-averages at electrode A25 as indicated with a red * on left (<b>b</b>), with time of maximum effect marked with red vertical line, window start marked with a solid black line, and stimulus offset marked with a dashed black line. Top are the grand-averages for thick, medium and thin, with stimulus onset marked at zero; bottom is the time-series of the PGI, with the blue horizontal line indicating the duration of the effect.</p>
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<p>Discomfort- by-decrease across partitions, negative cluster, DC-shift period. (<b>a</b>) Topographic maps through time for the whole period, with red crosses indicating the significant cluster, which corresponds to the blue region in (<b>a</b>), the colour bar on the right represents T-statistic on the topographic maps. (<b>b</b>) Median split (on Discomfort) grand-averages at electrode B16 as indicated with a red * on left (<b>b</b>), the left column of grand-averages is for the low group (i.e., low on discomfort factor), right column of grand-averages is for the high group (i.e., high on discomfort factor). Top is the time-series for the PGI for each partition, red (partition 1), green (partition 2), blue (partition 3) with maximum effect marked with a red vertical line, window start marked with a solid black line, and stimulus offset marked with a dashed black line; second row is the grand-averages for the medium stimulus for each partition, red (partition 1), green (partition 2), blue (partition 3); third, fourth and fifth rows present grand-averages for partitions 1, 2 and 3 (respectively), each showing thin, medium and thick. The most important time series comparisons are the PGI and medium stimuli ERPs, indicated with the red outline. The main feature that drives this interaction is a change in the high group from partition 1 to partitions 2/3. This comes out as a negative effect on a decrease across partitions. A negative decrease is an increase, which is what we observe: the response increases from partition 1 (red) to partitions 2/3 (green and blue). Additionally, since this increase is from negative towards zero, we can functionally view this as an habituation effect, i.e., an extreme negative-going response is tending towards what we take as stasis, which here is zero. This pattern is not observed for the low group. That is, the high group on both outlined rows displays the habituation effect, and the low group does not show any clear pattern.</p>
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<p>Discomfort- by-increase across the partitions, negative cluster, DC-shift period. (<b>a</b>) Topographic map through time for the whole period, with red crosses indicating the first significant cluster the colour bar on the right represents T-statistic on the topographic maps. (<b>b</b>) Median split (on Discomfort) grand-averages at electrode B5 as indicated with a red * on left (<b>b</b>), the left column of grand-averages is for the low group, right column for the high group. Top is the grand-average for the PGI for each partition, red (partition 1), green (partition 2), blue (partition 3), with maximum effect marked with a red vertical line, window start marked with a solid black line, and stimulus offset marked with a dashed black line; second row is the grand-average for the medium stimulus for each partition, red (partition 1), green (partition 2), blue (partition 3); third, fourth and fifth rows present grand-averages for partitions 1, 2 and 3 (respectively), each showing thin, medium and thick. Although not directly plotted, the second significant cluster is at an adjacent electrode to that plotted (<b>b</b>), i.e., A31 is next to B5 and can be seen (<b>a</b>) as the last two scalp maps with the red boxes around the clusters’ area. Accordingly, this second cluster is effectively an extension of the one depicted here and can be interpreted from the plots (<b>b</b>). The most important time series comparisons are the PGI and medium stimuli ERPs, indicated with the red outline. This makes the effects easy to see, with the low group (left column) on both outlined rows displaying the sensitisation (increasing) effect and the high group (right column) only showing its habituation pattern (decrease) for the PGI.</p>
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<p>Discomfort- by-decrease across the onsets, negative cluster, DC-shift period. (<b>a</b>) Topographic maps through time for the whole period, with red crosses indicating the significant cluster, which corresponds to the blue region in (<b>a</b>), the colour bar on the right represents T-statistic on the topographic maps. (<b>b</b>) Median split (on Discomfort) grand-averages at electrode B16 indicated with a red * on left (<b>b</b>); the left column of grand-averages is for the low group, right column of grand-averages is for the high group. Top are the grand-averages for the PGI for each partition, red (partition 1), green (partition 2), blue (partition 3) with maximum effect marked with a red vertical line, window start marked with a solid black line, and stimulus offset marked with a dashed black line; second row are the grand-averages for the medium stimulus for each partition, red (partition 1), green (partition 2), blue (partition 3); third, fourth and fifth rows present grand-averages for partitions 1, 2 and 3 (respectively), each showing thin, medium and thick. The most important time series comparisons are the PGI and medium stimuli ERPs, indicated with the red outline, The top row (<b>b</b>) shows the basic effect, which starts just before 1.5 s. We see a large positive-going change from onset-pair 4,5 to onset-pair 6,7 for the high group, but a negative-going change from onset-pair 2,3 to onset-pair 4,5, for the low group. Additionally, a similar, although weaker, effect can be observed for the medium stimulus for the high group (2nd row (<b>b</b>), right hand side), suggesting that the pattern for the high group is not just driven by changes in response for thick and thin, although the low group shows little difference between mediums (2nd row (<b>b</b>), left hand side), potentially indicating that the PGI effect in the low group (top row, left side) is driven by changes in thick and thin. That is, the high group on both outlined (see red rectangle) rows displays increasing effect, with the low group almost showing a habituation (decreasing) pattern.</p>
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<p>Discomfort- by-increase (through the partitions) by decrease (through the onsets), DC-shift period. (<b>a</b>) Topographic maps through time for the whole period, with red crosses indicating the significant cluster, the colour bar on the right represents T-statistic on the topographic maps. (<b>b</b>) Median split (on Discomfort) grand-averages at electrode B5 as indicated with a red * on right (<b>b</b>); the left panel of grand-averages is for the low group, right panel of grand-averages is for the high group. Top row (partition 1), third row (partition 2) and fifth row (partition 3) show grand-averages for the PGI for each onset, red (onsets 2,3), green (onsets 4,5), blue (onsets 6,7) with maximum effect marked with a red vertical line, window start marked with a solid black line, and stimulus offset marked with a dashed black line. Second row (partition 1), fourth row (partition 2) and sixth row (partition 3) are the grand-averages for just the medium stimulus for each onset, red (onsets 2,3), green (onsets 4,5), blue (onsets 6,7). (<b>b</b>) shows what underlies this effect. The first row (partition 1) shows a striking sensitisation effect for the high group (right side), with a substantially higher response in the final Onset-pair (6,7). This pattern is absent, and potentially reversed into a decrease pattern for the corresponding low group grand-averages (left side of first row). Additionally, this increase across Onsets for high and weak decrease for low in partition 1 is also present when we plot the medium alone (2nd row), suggesting the elevated Onset-pair 6,7 effect for the high group truly reflects hyper-excitation. In contrast, the remaining 4 rows (<b>b</b>), which correspond to partitions 2 and 3, exhibit no, or certainly much weaker, patterns of change through the onsets. Panels are colored in grey and white only to separate each different panel into different rows.</p>
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20 pages, 8833 KiB  
Article
Calcium Indicators with Fluorescence Lifetime-Based Signal Readout: A Structure–Function Study
by Tatiana R. Simonyan, Larisa A. Varfolomeeva, Anastasia V. Mamontova, Alexey A. Kotlobay, Andrey Y. Gorokhovatsky, Alexey M. Bogdanov and Konstantin M. Boyko
Int. J. Mol. Sci. 2024, 25(23), 12493; https://doi.org/10.3390/ijms252312493 - 21 Nov 2024
Viewed by 161
Abstract
The calcium cation is a crucial signaling molecule involved in numerous cellular pathways. Beyond its role as a messenger or modulator in intracellular cascades, calcium’s function in excitable cells, including nerve impulse transmission, is remarkable. The central role of calcium in nervous activity [...] Read more.
The calcium cation is a crucial signaling molecule involved in numerous cellular pathways. Beyond its role as a messenger or modulator in intracellular cascades, calcium’s function in excitable cells, including nerve impulse transmission, is remarkable. The central role of calcium in nervous activity has driven the rapid development of fluorescent techniques for monitoring this cation in living cells. Specifically, genetically encoded calcium indicators (GECIs) are the most in-demand molecular tools in their class. In this work, we address two issues of calcium imaging by designing indicators based on the successful GCaMP6 backbone and the fluorescent protein BrUSLEE. The first indicator variant (GCaMP6s-BrUS), with a reduced, calcium-insensitive fluorescence lifetime, has potential in monitoring calcium dynamics with a high temporal resolution in combination with advanced microscopy techniques, such as light beads microscopy, where the fluorescence lifetime limits acquisition speed. Conversely, the second variant (GCaMP6s-BrUS-145), with a flexible, calcium-sensitive fluorescence lifetime, is relevant for static measurements, particularly for determining absolute calcium concentration values using fluorescence lifetime imaging microscopy (FLIM). To identify the structural determinants of calcium sensitivity in these indicator variants, we determine their spatial structures. A comparative structural analysis allowed the optimization of the GCaMP6s-BrUS construct, resulting in an indicator variant combining calcium-sensitive behavior in the time domain and enhanced molecular brightness. Our data may serve as a starting point for further engineering efforts towards improved GECI variants with fine-tuned fluorescence lifetimes. Full article
(This article belongs to the Collection Feature Papers in Molecular Biophysics)
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Figure 1
<p>Schematics showing the design of chimeric proteins used in the study. At the top, the spatial structure of GCaMP6 is shown, with the mutations required for the EGFP modification. In the center, there is a linear scheme of the GCaMP-type backbone. The numbering of amino acid positions corresponds to those of the unmodified proteins (EGFP and calmodulin). At the bottom (left and right), the modified variants, GCaMP6s-BrUS and GCaMP6s-BrUS-145, are displayed, with the introduced modifications displayed below them.</p>
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<p>Calcium sensitivity of purified GCaMP6s, GCaMP6s-BrUS, and GCaMP6s-BrUS-145 measured in the intensiometric mode. (<b>A</b>) The dependence of fluorescence intensity at 510 nm (λ<sub>ex</sub> = 475 nm) on calcium concentration, expressed as [Ca<sup>2+</sup>]<sub>free</sub> (see <a href="#app1-ijms-25-12493" class="html-app">Supplementary Table S1</a> for details on the correspondence between [Ca<sup>2+</sup>]<sub>free</sub> and [CaEGTA]). (<b>B</b>) Column histogram displaying the relative fluorescence intensity changes observed within the [Ca<sup>2+</sup>]<sub>free</sub> range of 0–39 μM (corresponds to the [CaEGTA] range of 0–10 mM). Standard errors of the mean (S.E.M.) are shown for each data point (<span class="html-italic">n</span> = 3).</p>
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<p>The graph describing the dependence of the amplitude-weighted mean fluorescence lifetime of indicator variants on calcium concentration ([CaEGTA] and [Ca<sup>2+</sup>]<sub>free</sub>; see <a href="#app1-ijms-25-12493" class="html-app">Supplementary Table S1</a> for details). λ<sub>ex</sub> = 450 nm, repetition rate is 20 MHz. Standard errors of the mean (S.E.M.) are shown for each data point (<span class="html-italic">n</span> = 3).</p>
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<p>Comparison of GCaMP6s-BrUS and GCaMP6s-BrUS-145 structures. (<b>A</b>,<b>B</b>) Superposition of GCaMP6s-BrUS and GCaMP6s-BrUS-145 (magenta) structures from two views. Color scheme for GCaMP6s-BrUS is the following: EFGP domain—green, CaM domain—blue, M13 helix—orange, and the linker 314–319—light gray. Chromophore and calcium ions are shown in light and dark gray color for GCaMP6s-BrUS and GCaMP6s-BrUS-145, respectively. Red arrows point to the shift of the linker 314–319 (<b>A</b>) and the C-lobe of the CaM domain (<b>B</b>). (<b>C</b>–<b>F</b>) Differences in the conformation of residues surrounding the chromophore. Panels (<b>C</b>,<b>E</b>) represent GCaMP6s-BrUS structure and (<b>D</b>,<b>F</b>) GCaMP6s-BrUS-145. Solvent molecules are shown as red spheres. Hydrogen bonds are depicted as dashed blue lines.</p>
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<p>Calcium sensitivity of GCaMP6s-BrUS-389K fluorescence. (<b>A</b>) The dependence of fluorescence intensity at 510 nm (λ<sub>ex</sub> = 475 nm) on calcium concentration, expressed as [Ca<sup>2+</sup>]<sub>free</sub>. (<b>B</b>) The graph describing the dependence of the amplitude-weighted mean fluorescence lifetime on calcium concentration ([CaEGTA] and [Ca<sup>2+</sup>]<sub>free</sub>). λ<sub>ex</sub> = 450 nm, repetition rate is 20 MHz. Standard errors of the mean (S.E.M.) are shown for each data point (<span class="html-italic">n</span> = 3).</p>
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<p>Calcium sensitivity of GCaMP6s-BrUS-389K/398G fluorescence. (<b>A</b>) The dependence of fluorescence intensity at 510 nm (λ<sub>ex</sub> = 475 nm) on calcium concentration, expressed as [Ca<sup>2+</sup>]<sub>free</sub>. (<b>B</b>) The graph describing the dependence of the amplitude-weighted mean fluorescence lifetime on calcium concentration ([CaEGTA] and [Ca<sup>2+</sup>]<sub>free</sub>). λ<sub>ex</sub> = 450 nm, repetition rate is 20 MHz. Standard errors of the mean (S.E.M.) are shown for each data point (<span class="html-italic">n</span> = 3).</p>
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34 pages, 16144 KiB  
Article
Unveiling the Intra-Annual and Inter-Annual Spatio-Temporal Dynamics of Sediment Inflow to Rivers and Driving Factors in Cloud-Prone Regions: A Case Study in Minjiang River Basin, China
by Xiaoqin Wang, Zhichao Yu, Lin Li, Mengmeng Li, Jinglan Lin, Lifang Tang, Jianhui Chen, Haihan Lin, Miao Chen, Shilai Jin, Yunzhi Chen and Xiaocheng Zhou
Water 2024, 16(22), 3339; https://doi.org/10.3390/w16223339 (registering DOI) - 20 Nov 2024
Viewed by 249
Abstract
Accurately delineating sediment export dynamics using high-quality vegetation factors remains challenging due to the spatio-temporal resolution imbalance of single remote sensing data and persistent cloud contamination. To address these challenges, this study proposed a new framework for estimating and analyzing monthly sediment inflow [...] Read more.
Accurately delineating sediment export dynamics using high-quality vegetation factors remains challenging due to the spatio-temporal resolution imbalance of single remote sensing data and persistent cloud contamination. To address these challenges, this study proposed a new framework for estimating and analyzing monthly sediment inflow to rivers in the cloud-prone Minjiang River Basin. We leveraged multi-source remote sensing data and the Continuous Change Detection and Classification model to reconstruct monthly vegetation factors at 30 m resolution. Then, we integrated the Chinese Soil Loss Equation model and the Sediment Delivery Ratio module to estimate monthly sediment inflow to rivers. Lastly, the Optimal Parameters-based Geographical Detector model was harnessed to identify factors affecting sediment export. The results indicated that: (1) The simulated sediment transport modulus showed a strong coefficient of determination (R2 = 0.73) and a satisfactory Nash–Sutcliffe efficiency coefficient (0.53) compared to observed values. (2) The annual sediment inflow to rivers exhibited a spatial distribution characterized by lower levels in the west and higher in the east. The monthly average sediment value from 2016 to 2021 was notably high from March to July, while relatively low from October to January. (3) Erosive rainfall was a decisive factor contributing to increased sediment entering the rivers. Vegetation factors, manifested via the quantity (Fractional Vegetation Cover) and quality (Leaf Area Index and Net Primary Productivity) of vegetation, exert a pivotal influence on diminishing sediment export. Full article
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<p>Overview of the study region. (<b>a</b>) Location of the study area in Fujian; (<b>b</b>) Land use in 2021; (<b>c</b>) Total annual precipitation from 2016 to 2021; (<b>d</b>) Multi-year monthly average precipitation from 2016 to 2021; (<b>e</b>) Delineation of small watersheds in the MJRB.</p>
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<p>Available monthly observations of GF-1 WFV, Landsat-8 OLI, Sentinel-2 MSI with cloud cover less than 10% from 2016 to 2021.</p>
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<p>An overview of the workflow in this study (Notes: *: <span class="html-italic">p</span> &lt; 0.05; ***: <span class="html-italic">p</span> &lt; 0.001).</p>
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<p>Spatial distribution of driving factors in 2021. (<b>a</b>) FVC; (<b>b</b>) LAI; (<b>c</b>) NPP; (<b>d</b>) BRI; (<b>e</b>) PF; (<b>f</b>) PT; (<b>g</b>) HAILS; (<b>h</b>) LRR; (<b>i</b>) PB.</p>
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<p>Validation of the model estimation results.</p>
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<p>Demonstrations of turbidity change for typical river sections from 2016 to 2021. (<b>1</b>) River section located in the upper reaches of the MJRB within the Futunxi-Jinxi sub-basin; (<b>2</b>) River section situated in the midstream within the Gutianxi sub-basin; (<b>3</b>) Main stream of the Minjiang River in the lower reaches.</p>
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<p>Proportion of different turbidity for river sections from 2016 to 2021.</p>
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<p>Distribution of sediment inflow to rivers from 2016 to 2021.</p>
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<p>Statistics of annual sediment inflow to rivers for small watersheds from 2016 to 2021. The bottom and top edges of the boxes indicate the 25th and 75th percentiles, respectively. The horizontal bold blue lines denote the median. The dashed lines extended from the interquartile with length of 1.5 times box width. The dots represent the mean.</p>
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<p>Spatial distribution of annual sediment inflow to rivers from 2016 to 2021. (Noted: The values presented represent the actual annual sediment load).</p>
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<p>Spatial distribution of annual average sediment inflow to rivers (<b>a</b>) and annual average sediment influx ratio to rivers (<b>b</b>) from 2016 to 2021.</p>
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<p>Overview of the Gutianxi sub-basin. (<b>a</b>) Elevation of the Gutianxi sub-basin; (<b>b</b>) High-resolution remote sensing image from Google Earth.</p>
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<p>Distribution of monthly average sediment inflow to rivers from 2016 to 2021.</p>
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<p>Statistics of monthly average sediment inflow to rivers for small watersheds from 2016 to 2021. The bottom and top edges of the boxes indicate the 25th and 75th percentiles, respectively. The horizontal bold blue lines denote the median. The dashed lines extended from the interquartile with length of 1.5 times box width. The dots represent the mean.</p>
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<p>Multi-year monthly average sediment inflow to rivers and erosive rainfall/R factor from 2016 to 2021.</p>
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<p>Correlation between sediment inflow to rivers and other factors from 2016 to 2021 (Notes: SR: Sediment inflow to Rivers; FVC: Fractional Vegetation Coverage; LAI: Leaf Area Index; NPP: Net Primary Productivity; BRI: Biological Richness Index; PF: Proportion of Forest Land; ER: erosive rainfall; HAILS: Human Activity Intensity of Land Surface; LRR: Land Reclamation Rate; PB: Proportion of Built-up land; *: <span class="html-italic">p</span> &lt; 0.05; ***: <span class="html-italic">p</span> &lt; 0.001).</p>
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<p>Spatial distribution of dominant factor to sediment inflow to rivers (<b>a</b>) and contribution (q-value) of different factors to sediment inflow to rivers (<b>b</b>) from 2016 to 2021.</p>
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<p>Spatial distribution of dominant factor combinations to sediment inflow to rivers.</p>
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<p>Multi-year monthly average FVC and B factor from 2016 to 2021.</p>
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<p>Comparison of measured sediment transport modulus with simulated values calculated separately using the CSLE + SDR and RUSLE + SDR models.</p>
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21 pages, 7206 KiB  
Article
Remote Sensing Fine Estimation Model of PM2.5 Concentration Based on Improved Long Short-Term Memory Network: A Case Study on Beijing–Tianjin–Hebei Urban Agglomeration in China
by Yiye Ji, Yanjun Wang, Cheng Wang, Xuchao Tang and Mengru Song
Remote Sens. 2024, 16(22), 4306; https://doi.org/10.3390/rs16224306 - 19 Nov 2024
Viewed by 258
Abstract
The accurate prediction of PM2.5 concentration across extensive temporal and spatial scales is essential for air pollution control and safeguarding public health. To address the challenges of the uneven coverage and limited number of traditional PM2.5 ground monitoring networks, the low [...] Read more.
The accurate prediction of PM2.5 concentration across extensive temporal and spatial scales is essential for air pollution control and safeguarding public health. To address the challenges of the uneven coverage and limited number of traditional PM2.5 ground monitoring networks, the low inversion accuracy of PM2.5 concentration, and the incomplete understanding of its spatiotemporal dynamics, this study proposes a refined PM2.5 concentration estimation model, Bi-LSTM-SA, integrating multi-source remote sensing data. First, utilizing multi-source remote sensing data, such as MODIS aerosol optical depth (AOD) products, meteorological data, and PM2.5 monitoring sites, AERONET AOD was used to validate the accuracy of the MODIS AOD data. Variables including temperature (TEMP), relative humidity (RH), surface pressure (SP), wind speed (WS), and total precipitation (PRE) were selected, followed by the application of the variance inflation factor (VIF) and Pearson’s correlation coefficient (R) for variable screening. Second, to effectively capture temporal dependencies and emphasize key features, an improved Long Short-Term Memory Network (LSTM) model, Bi-LSTM-SA, was constructed by combining a bidirectional LSTM (Bi-LSTM) model with a self-adaptive attention mechanism (SA). This model was evaluated through ablation and comparative experiments using three cross-validation methods: sample-based, temporal, and spatial. The effectiveness of this method was demonstrated on Beijing–Tianjin–Hebei urban agglomeration, achieving a coefficient of determination (R2) of 0.89, root mean squared error (RMSE) of 12.76 μg/m3, and mean absolute error (MAE) of 8.27 μg/m3. Finally, this model was applied to predict PM2.5 concentration on Beijing–Tianjin–Hebei urban agglomeration in 2023, revealing the characteristics of its spatiotemporal evolution. Additionally, the results indicated that this model performs exceptionally well in hourly PM2.5 concentration forecasting and can be used for PM2.5 concentration hourly prediction tasks. This study provides technical support for the large-scale, accurate remote sensing inversion of PM2.5 concentration and offers fundamental insights for regional atmospheric environmental protection. Full article
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<p>Research flowchart in this study.</p>
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<p>The distribution of PM<sub>2.5</sub> monitoring stations on Beijing–Tianjin–Hebei urban agglomeration.</p>
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<p>Verification results of MODIS AOD and AERONET AOD. Among them, N represents the number of matches.</p>
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<p>Self-adaptive attention mechanism processing flow.</p>
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<p>Bi-LSTM-SA network structure.</p>
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<p>Density scatter plot of the ablation experiment results. Among them, the units of RMSE and MAE are μg/m<sup>3</sup>. In this figure, the three rows indicate the fitting results under sample-based, spatial, and temporal cross-validation (CV), while the four columns indicate the fitting results of basic LSTM, Bi-LSTM, LSTM-SA and Bi-LSTM-SA models, respectively. The solid black line in the figure represents the fitted straight line of y = x, and the solid red line represents the best fit line of the linear regression.</p>
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<p>The density scatter plot of the comparative experiment results. Among them, the units of RMSE and MAE are μg/m<sup>3</sup>. In this figure, the three rows indicate the fitting results under sample-based, spatial and temporal cross-validation (CV), while the four columns indicate the fitting results of Transformer, CNN, RF and Bi-LSTM-SA models, respectively. The solid black line in the figure represents the fitted straight line of y = x, and the solid red line represents the best fit line of the linear regression.</p>
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<p>Fit comparison. (<b>a</b>): Fit result of Bi-LSTM-SA model predictions vs. observed values; (<b>b</b>): fit result of CHAP PM<sub>2.5</sub> data vs. observed values; N: the number of valid data samples. The units of RMSE and MAE are μg/m<sup>3</sup>. The solid black line in the figure represents the fitted straight line of y = x, and the solid red line represents the best fit line of the linear regression.</p>
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<p>The spatio-temporal pattern of the monthly average PM<sub>2.5</sub> concentrations in the Beijing–Tianjin–Hebei urban agglomeration for 2023.</p>
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<p>The comparison of the five models’ PM<sub>2.5</sub> concentration forecasting performance across different time windows within the next 72 h. The figure represents the R<sup>2</sup>, RMSE and MAE between the predicted and observed values for each station under the same test set.</p>
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<p>PM<sub>2.5</sub> prediction trends versus actual trends at different stations and times, (<b>a</b>–<b>d</b>) representing different stations.</p>
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11 pages, 3124 KiB  
Article
Interspecific Relationship Between Monochamus alternatus Hope and Arhopalus rusticus (L.) in Pinus thunbergii Affected by Pine Wilt Disease
by Yingchao Ji, Chenyu Song, Long Chen, Xue Zheng, Chunyan Jia and Yanxue Liu
Forests 2024, 15(11), 2037; https://doi.org/10.3390/f15112037 - 19 Nov 2024
Viewed by 203
Abstract
Monochamus alternatus Hope and Arhopalus rusticus (L.) are important stem-boring pests that co-occur on weakened Pinus spp. Their larvae damage the xylem and phloem of the trunks and branches. At present, the consequences of the interspecific relationship between two longicorn beetles on the [...] Read more.
Monochamus alternatus Hope and Arhopalus rusticus (L.) are important stem-boring pests that co-occur on weakened Pinus spp. Their larvae damage the xylem and phloem of the trunks and branches. At present, the consequences of the interspecific relationship between two longicorn beetles on the same host of Pinus trees are unclear. The population dynamics and spatial distribution of these two species on Pinus thunbergii trees were investigated to clarify the ecological niches and interspecific relationship of two longicorn beetles on the different degrees of decline in P. thunbergii trees. The results showed temporal niche overlap values from 0.02 ± 0.01 to 0.05 ± 0.02, suggesting a very high degree of temporal ecological niche segregation and no competition in temporal niche resources. There is significant interspecific competition between the two longicorn beetles in spatial distribution, and the spatial niche overlap values are 0.67 ± 0.11 and 0.61 ± 0.09 in the middle and late stages of the decline in P. thunbergii trees, respectively. Full article
(This article belongs to the Section Forest Health)
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<p>The characteristics of the four decline stages of <span class="html-italic">P. thunbergia</span>: (<b>A</b>) early stage of decline, (<b>B</b>) middle stage of decline, (<b>C</b>) later stage of decline, and (<b>D</b>) wilting death stage.</p>
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<p>Two longicorn beetle larvae and the stump of <span class="html-italic">Pinus thunbergii</span>. (<b>A</b>) <span class="html-italic">M. alternatus</span> larvae, (<b>B</b>) <span class="html-italic">A. rusticus</span> larvae, (<b>C</b>) stump in the current year, and (<b>D</b>) stump in the next year.</p>
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<p>Seasonal dynamics of the two species of longicorn beetles in a <span class="html-italic">P. thunbergii</span> forest: (<b>A</b>) population in 2019; (<b>B</b>) population in 2020.</p>
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<p>The within-trunk distribution of <span class="html-italic">Monochamus alternatus</span> and <span class="html-italic">Arhopalus rusticus</span> larvae in their host plant <span class="html-italic">P. thunbergii</span> at various vigor levels: (<b>A</b>) early stage of decline, (<b>B</b>) middle stage of decline, (<b>C</b>) stage of decline, and (<b>D</b>) dead. Data in the figure are mean ± SD of five replications (<span class="html-italic">n</span> = 5). Lowercase letters and capital letters indicate significant differences of distribution proportion at different heights for <span class="html-italic">M. alternatus</span> and <span class="html-italic">A. rusticus</span> larvae, respectively (<span class="html-italic">p</span> &lt; 0.05).</p>
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16 pages, 10577 KiB  
Article
Designing a Multitemporal Analysis of Land Use Changes and Vegetation Indices to Assess the Impacts of Severe Forest Fires Before Applying Control Measures
by Casandra Muñoz-Gómez and Jesús Rodrigo-Comino
Forests 2024, 15(11), 2036; https://doi.org/10.3390/f15112036 - 18 Nov 2024
Viewed by 538
Abstract
Forest fires represent a significant intersection between nature and society, often leading to the loss of natural resources, soil nutrients, and economic opportunities, as well as causing desertification and the displacement of communities. Therefore, the objective of this work is to analyze the [...] Read more.
Forest fires represent a significant intersection between nature and society, often leading to the loss of natural resources, soil nutrients, and economic opportunities, as well as causing desertification and the displacement of communities. Therefore, the objective of this work is to analyze the multitemporal conditions of a sixth-generation forest fire through the use and implementation of tools such as remote sensing, photointerpretation with geographic information systems (GISs), thematic information on land use, and the use of spatial indices such as the Normalized Difference Vegetation Index (NDVI), the Normalized Burned Ratio (NBR), and its difference (dNBR) with satellite images from Sentinel-2. To improve our understanding of the dynamics and changes that occurred due to the devastating forest fire in Los Guájares, Granada, Spain, in September 2022, which affected 5194 hectares and had a perimeter of 150 km, we found that the main land use in the study area was forest, followed by agricultural areas which decreased from 1956 to 2003. We also observed the severity of burning, shown with the dNBR, reflecting moderate–low and moderate–high levels of severity. Health and part of the post-fire recovery process, as indicated by the NDVI, were also observed. This study provides valuable information on the spatial and temporal dimensions of forest fires, which will favor informed decision making and the development of effective prevention strategies. Full article
(This article belongs to the Topic Application of Remote Sensing in Forest Fire)
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<p>Localization of the study area and photographs during the fieldwork campaign.</p>
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<p>Maps of elevation and inclination of the study area.</p>
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<p>Land use maps showing the changes among selected dates.</p>
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<p>Maps considering land use changes between specific intervals of years.</p>
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<p>Satellite images with natural color from March 2022 to September 2022.</p>
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<p>Satellite images with natural color from October 2022 to March 2023.</p>
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<p>Satellite images with natural color from August to October in 2021 and 2023.</p>
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<p>Normalize Difference Vegetation Index (NDVI) from March 2022 to September 2022.</p>
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<p>Normalize Difference Vegetation Index (NDVI) from October 2022 to March 2023.</p>
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<p>Normalized Difference Vegetation Index (NDVI) from August to October in 2021 and 2023.</p>
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<p>Normalized Burn Ratio (NBR) from August and October 2022.</p>
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<p>Difference Normalized Burn Ratio (dNBR) from August and October 2022.</p>
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24 pages, 1655 KiB  
Article
The Spatial–Temporal Evolution and Impact Mechanism of Cultivated Land Use in the Mountainous Areas of Southwest Hubei Province, China
by Zhengxiang Wu, Qingbin Fan, Wen Li and Yong Zhou
Land 2024, 13(11), 1946; https://doi.org/10.3390/land13111946 - 18 Nov 2024
Viewed by 290
Abstract
Changes in cultivated land use significantly impact food production capacity, which in turn affects food security. Therefore, accurately understanding the spatial and temporal variations in cultivated land use is critical for strategic decision-making regarding national food security. Since the second national soil survey [...] Read more.
Changes in cultivated land use significantly impact food production capacity, which in turn affects food security. Therefore, accurately understanding the spatial and temporal variations in cultivated land use is critical for strategic decision-making regarding national food security. Since the second national soil survey was conducted in around 1980, China has implemented major efforts, such as a nationwide soil testing and fertilization project in around 2005 and the establishment of the National Standards for Cultivated Land Quality Grading in 2016. However, limited research has focused on how cultivated land use has changed during these periods and the mechanisms driving these changes. This study, using Enshi Prefecture in the mountainous region of southwestern Hubei Province as a case study, examines the spatiotemporal changes in cultivated land use during 1980–2018. Land use data from 1980, 2005, and 2018 were combined with statistical yearbook data from Enshi Prefecture, and remote sensing and GIS technology were applied. Indicators such as the dynamic degree of cultivated land use, the relative rate of change in cultivated land use, and a Geoscience Information Atlas model were used to explore these changes. Additionally, principal component analysis was employed to examine the mechanisms influencing these changes. The results show that (1) the area of cultivated land in Enshi Prefecture increased slightly from 1980 to 2005, while from 2005 to 2018, it significantly decreased; compared with the earlier period, the transformation of land use types during 2005–2018 was more intense; (2) the increase in cultivated land area from 1980 to 2005 was mainly due to deforestation, the creation of farmland from lakes, and the reclamation of wasteland, while the decrease in land area was primarily attributed to the conversion of farmland back to forests and grassland. From 2005 to 2018, the main drivers for the increase in cultivated land were deforestation and the reclamation of wasteland, while the return of farmland to forests remained the primary reason for the decrease in land area; (3) from 1980 to 2005, the dynamic degree of cultivated land use in each county and city of Enshi Prefecture was generally low. However, between 2005 and 2018, the dynamic degree increased in most counties and cities except Enshi City and Xianfeng County; (4) there were significant variations in the relative rate of change in cultivated land utilization across counties and cities from 1980 to 2005. However, from 2005 to 2018, the relative rate of change decreased in all counties and cities compared to the previous period; (5) since 1980, nearly 50% of the cultivated land in Enshi Prefecture has undergone land classification conversion, with frequent shifts between different land classes; and (6) economic development, population growth, capital investment, food production, and production efficiency are the dominant socioeconomic factors driving changes in cultivated land use in Enshi Prefecture. The results of this study can provide a scientific basis for the protection and optimization of cultivated land resources in the mountainous regions of southwestern Hubei Province. Full article
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<p>Study area location and elevation distribution.</p>
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<p>Atlas of cultivated land use change patterns in Enshi Prefecture from 1980 to 2018.</p>
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<p>Changes in Population and Cultivated Land in Enshi Prefecture from 1980 to 2018.</p>
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<p>Economic Development and Changes in Enshi Prefecture from 1980 to 2018.</p>
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<p>Changes in the total output value of agriculture, forestry, animal husbandry, and fishery in Enshi Prefecture and the income of farmers and residents from 1980 to 2018.</p>
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28 pages, 2534 KiB  
Review
NMDA Receptors in Neurodevelopmental Disorders: Pathophysiology and Disease Models
by Roshan Tumdam, Yara Hussein, Tali Garin-Shkolnik and Shani Stern
Int. J. Mol. Sci. 2024, 25(22), 12366; https://doi.org/10.3390/ijms252212366 - 18 Nov 2024
Viewed by 377
Abstract
N-methyl-D-aspartate receptors (NMDARs) are critical components of the mammalian central nervous system, involved in synaptic transmission, plasticity, and neurodevelopment. This review focuses on the structural and functional characteristics of NMDARs, with a particular emphasis on the GRIN2 subunits (GluN2A-D). The diversity of GRIN2 [...] Read more.
N-methyl-D-aspartate receptors (NMDARs) are critical components of the mammalian central nervous system, involved in synaptic transmission, plasticity, and neurodevelopment. This review focuses on the structural and functional characteristics of NMDARs, with a particular emphasis on the GRIN2 subunits (GluN2A-D). The diversity of GRIN2 subunits, driven by alternative splicing and genetic variants, significantly impacts receptor function, synaptic localization, and disease manifestation. The temporal and spatial expression of these subunits is essential for typical neural development, with each subunit supporting distinct phases of synaptic formation and plasticity. Disruptions in their developmental regulation are linked to neurodevelopmental disorders, underscoring the importance of understanding these dynamics in NDD pathophysiology. We explore the physiological properties and developmental regulation of these subunits, highlighting their roles in the pathophysiology of various NDDs, including ASD, epilepsy, and schizophrenia. By reviewing current knowledge and experimental models, including mouse models and human-induced pluripotent stem cells (hiPSCs), this article aims to elucidate different approaches through which the intricacies of NMDAR dysfunction in NDDs are currently being explored. The comprehensive understanding of NMDAR subunit composition and their mutations provides a foundation for developing targeted therapeutic strategies to address these complex disorders. Full article
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<p>Structural composition of the NMDA receptors; (<b>A</b>) Graphical representation of the subunit composition of NMDA receptors. Each monomer of the receptor contains four functional domains, the amino-terminal domain (ATD), ligand-binding domain (LBD), transmembrane domain (TMD), and C-terminal domain. (<b>B</b>) Ion channel activity of AMPA and Kainate receptors (members of iGluR family). (<b>C</b>) Crystal structure displaying 3D conformation of the heterotetramer NMDA receptor containing GluN1 and GluN2B subunits (PDB: 6WHX); GluN3 subunits also form functional receptors with GluN1 subunit. (<b>D</b>) The proposed GluD2 ion channel activity mechanism is through interaction with pre-synaptic linker proteins; the representation is adapted from Carillo et al. SciAdv, 2021 [<a href="#B12-ijms-25-12366" class="html-bibr">12</a>].</p>
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<p>Activation of the “coincidence receptor”. (<b>A</b>) Initial interaction of AMPAR (blue) and NMDAR (Purple) with pre-synaptic glutamate; the receptors are inactive at the resting membrane potential (−70 mV); upon interaction with the presynaptic glutamate, NMDAR remains inactive still since Mg<sup>2+</sup> is blocking the ion channel pore, while AMPA receptor allows in-flow of Na<sup>+</sup> ions initiating the depolarization of the postsynaptic membrane. (<b>B</b>) Depolarization of the postsynaptic membrane facilitates the release of Mg<sup>2+</sup> ions from the NMAR ion channel pore, activating the receptor. (<b>C</b>) Activation of NMDAR and repetitive presynaptic glutamate release leads to increased in-flow of Ca<sup>2+</sup> and Na<sup>+</sup> ions into the postsynaptic neurons. The representation is adapted from Sprengel et al. 2022 [<a href="#B43-ijms-25-12366" class="html-bibr">43</a>]. This fundamental characteristic of NMDA receptors is disrupted in NDDs, where disease-associated variants are distributed across various domains of the GRIN proteins within NMDARs, impacting multiple physiological properties and leading to either receptor hypofunction or hyperfunction. Missense variants within the transmembrane helix may alter NMDAR surface expression and modify receptor sensitivity to endogenous agonists and inhibitors [<a href="#B2-ijms-25-12366" class="html-bibr">2</a>]. Likewise, variants in the extracellular ATD and LBD regions are often linked to receptor loss of function in DD/ID patients [<a href="#B27-ijms-25-12366" class="html-bibr">27</a>]. These alterations impact the neurons’ downstream calcium signaling, which affects long-term potentiation and synaptic plasticity in NDD patients.</p>
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<p>Schematics of the eight functional isoforms of the GluN1 subunit resulting from alternative RNA splicing. Color coding represents the splicing sites at exons 5, 21, and 22 or 22’, denoted as cassettes N1, C1, C2, and C2’, respectively. N1 cassette site is in the amino-terminal domain, whereas C1, C2, and C2’ sites are in the C-terminal domain. The schematics are adapted from Li et al., PNAS, 2021 [<a href="#B45-ijms-25-12366" class="html-bibr">45</a>].</p>
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<p>Modeling neurodevelopmental disorders using patient-derived iPSCs. The illustration depicts the general protocol for modelling human neurodevelopmental disorders (NDDs) using patient-derived induced pluripotent stem cells (iPSCs). The iPSCs can be derived from the fibroblasts, PBMCs, or lymphoblastoid cell lines (LCLs) of the patients as well as the healthy subjects (Isogenic controls). These iPSCs provide a platform to study the disease biology and explore novel therapeutic strategies through various techniques that can lead to the development of personalized and more effective treatment options for patients suffering with different NDDs. The schematics of the figure are adapted from Liu et al., Development, 2018 [<a href="#B148-ijms-25-12366" class="html-bibr">148</a>]. iPSC-derived neurons from ASD patients frequently exhibit reduced synaptic activity and altered excitatory/inhibitory signaling balance, both of which are critical to understanding synaptic dysfunctions characteristic of ASD [<a href="#B149-ijms-25-12366" class="html-bibr">149</a>,<a href="#B150-ijms-25-12366" class="html-bibr">150</a>,<a href="#B151-ijms-25-12366" class="html-bibr">151</a>,<a href="#B152-ijms-25-12366" class="html-bibr">152</a>]. With their ability to model complex synaptic processes, aberrant connectivity, and neurotransmitter imbalances, hiPSC-derived neurons serve as invaluable tools for dissecting the molecular mechanisms underlying neuropsychiatric disorders such as ASD, schizophrenia, bipolar disorder, and ID [<a href="#B153-ijms-25-12366" class="html-bibr">153</a>,<a href="#B154-ijms-25-12366" class="html-bibr">154</a>,<a href="#B155-ijms-25-12366" class="html-bibr">155</a>,<a href="#B156-ijms-25-12366" class="html-bibr">156</a>,<a href="#B157-ijms-25-12366" class="html-bibr">157</a>]. Integration-free methods for iPSC generation avoids genomic integration of vectors, thereby preserving genetic integrity and reducing tumorigenic risks [<a href="#B158-ijms-25-12366" class="html-bibr">158</a>]. Consequently, several groups have successfully adopted this method for modeling different neurological disorders. For instance, iPSCs were generated by reprogramming fibroblasts derived from a Phelan-McDermid syndrome (PMS) patient, harboring an insertion mutation in SHANK3 (C.3679insG) [<a href="#B159-ijms-25-12366" class="html-bibr">159</a>]. The iPSCs were observed to express the pluripotency markers, differentiate into the three germ layers, retain the disease-causing mutation, and display normal karyotypes. Therefore, this technology allows researchers to explore the functional properties of cellular factors involved in the pathology of NDDs, which can be translated into a patient specific therapeutic intervention.</p>
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23 pages, 28843 KiB  
Article
Spatiotemporal Dynamics and Driving Factors of Soil Salinization: A Case Study of the Yutian Oasis, Xinjiang, China
by Shiqin Li, Ilyas Nurmemet, Jumeniyaz Seydehmet, Xiaobo Lv, Yilizhati Aili and Xinru Yu
Land 2024, 13(11), 1941; https://doi.org/10.3390/land13111941 - 18 Nov 2024
Viewed by 320
Abstract
Soil salinization is a critical global environmental issue, exacerbated by climatic and anthropogenic factors, and posing significant threats to agricultural productivity and ecological stability in arid regions. Therefore, remote sensing-based dynamic monitoring of soil salinization is crucial for timely assessment and effective mitigation [...] Read more.
Soil salinization is a critical global environmental issue, exacerbated by climatic and anthropogenic factors, and posing significant threats to agricultural productivity and ecological stability in arid regions. Therefore, remote sensing-based dynamic monitoring of soil salinization is crucial for timely assessment and effective mitigation strategies. This study used Landsat imagery from 2001 to 2021 to evaluate the potential of support vector machine (SVM) and classification and regression tree (CART) models for monitoring soil salinization, enabling the spatiotemporal mapping of soil salinity in the Yutian Oasis. In addition, the land use transfer matrix and spatial overlay analysis were employed to comprehensively analyze the spatiotemporal trends of soil salinization. The geographical detector (Geo Detector) tool was used to explore the driving factors of the spatiotemporal evolution of salinization. The results indicated that the CART model achieved 5.3% higher classification accuracy than the SVM, effectively mapping the distribution of soil salinization and showing a 26.76% decrease in salinized areas from 2001 to 2021. Improvements in secondary salinization and increased vegetation coverage were the primary contributors to this reduction. Geo Detector analysis highlighted vegetation (NDVI) as the dominant factor, and its interaction with soil moisture (NDWI) has a significant impact on the spatial and temporal distribution of soil salinity. This study provides a robust method for monitoring soil salinization, offering critical insights for effective salinization management and sustainable agricultural practices in arid regions. Full article
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<p>Overview of the study area. (<b>A</b>) Map of China. (<b>B</b>) Map of Xinjiang, China, Yutian County, and study area. (<b>C</b>) Map of the study area. Figure (<b>C</b>) shows Landsat8 OLI 15 July 2021 remote sensing image of the study area.</p>
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<p>Workflow.</p>
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<p>Sample separability and overall classification accuracy.</p>
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<p>2021 Comparison of soil salinity classification details. BB: bare land desert and building; VG: vegetation; MS: moderately salinization soil; HS: highly salinization soil; (<b>A</b>) Landsat8 image for case A; (<b>B</b>) Landsat8 image for case B.</p>
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<p>Spatiotemporal distribution of soil salinization from 2001 to 2021.</p>
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<p>The weighting of different land types and calculation methods for spatiotemporal evolution of soil salinization. SS indicates slightly salinized soil, MS indicates moderately salinized soil, HS indicates highly salinized soil, Other indicates BB, VG, WB, BB indicates bare land building and desert, VG indicates vegetation, WB indicates water body.</p>
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<p>Spatial-temporal evolution of soil salinization in 2001–2021.</p>
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<p>2001–2021 Land transfer Sankey diagram. SS indicates slightly salinized soil, MS indicates moderately salinized soil, HS indicates highly salinized soil, BB indicates bare land, desert, and building, VG indicates vegetation, and WB indicates water body.</p>
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<p>Factor detection and interaction detection results; DDI indicates desertification difference index; TVDI indicates temperature vegetation drought index; S1, S2, SI2 indicates salinity index; NDVI indicates normalized difference salinity index, NDWI indicates normalized difference water index, LST indicates land surface temperature, CSI indicates comprehensive salinity index.</p>
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<p>Landsat8 OLI compared to Planet scope for salinization soil classification in 2021; MS indicates moderately salinized soil, HS indicates highly salinized soil, BB indicates Bare land building and desert, VG indicates Vegetation.</p>
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20 pages, 3073 KiB  
Article
Successful Precipitation Downscaling Through an Innovative Transformer-Based Model
by Fan Yang, Qiaolin Ye, Kai Wang and Le Sun
Remote Sens. 2024, 16(22), 4292; https://doi.org/10.3390/rs16224292 - 18 Nov 2024
Viewed by 272
Abstract
In this research, we introduce a novel method leveraging the Transformer architecture to generate high-fidelity precipitation model outputs. This technique emulates the statistical characteristics of high-resolution datasets while substantially lowering computational expenses. The core concept involves utilizing a blend of coarse and fine-grained [...] Read more.
In this research, we introduce a novel method leveraging the Transformer architecture to generate high-fidelity precipitation model outputs. This technique emulates the statistical characteristics of high-resolution datasets while substantially lowering computational expenses. The core concept involves utilizing a blend of coarse and fine-grained simulated precipitation data, encompassing diverse spatial resolutions and geospatial distributions, to instruct Transformer in the transformation process. We have crafted an innovative ST-Transformer encoder component that dynamically concentrates on various regions, allocating heightened focus to critical spatial zones or sectors. The module is capable of studying dependencies between different locations in the input sequence and modeling at different scales, which allows it to fully capture spatiotemporal correlations in meteorological element data, which is also not available in other downscaling methods. This tailored module is instrumental in enhancing the model’s ability to generate outcomes that are not only more realistic but also more consistent with physical laws. It adeptly mirrors the temporal and spatial distribution in precipitation data and adeptly represents extreme weather events, such as heavy and enduring storms. The efficacy and superiority of our proposed approach are substantiated through a comparative analysis with several cutting-edge forecasting techniques. This evaluation is conducted on two distinct datasets, each derived from simulations run by regional climate models over a period of 4 months. The datasets vary in their spatial resolutions, with one featuring a 50 km resolution and the other a 12 km resolution, both sourced from the Weather Research and Forecasting (WRF) Model. Full article
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<p>Schematic diagram of the suggested STTA framework. In this diagram, the 0 box represents a specific feature or a processed feature map, and the * represents multiplying this feature map by another feature map element by element. This operation is used in neural networks to achieve the weighting of features, where different weights can emphasize or suppress different parts of the input features. In the attention mechanism, this operation can be used to apply attention weights to a feature map to highlight important features and suppress unimportant ones.</p>
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<p>Details of the inception module.</p>
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<p>Encoder module utilized to preprocess the low-resolution input data, ensuring it is appropriately formatted for subsequent transmission to the network.</p>
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<p>Details of the ST-Transformer module depicted in <a href="#remotesensing-16-04292-f001" class="html-fig">Figure 1</a>. The * in the 0 box usually stands for an element-wise multiplication operation, also known as the Hadamard product or dot product, where it means multiplying this feature graph by another feature graph element by element.</p>
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<p>Seven subregions of CONUS.</p>
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<p>Ground Truth and precipitation forecast output of Interpolator, ESPCN, SRCNN, Encoded-CNN, Direct-CNN, and STTA.</p>
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<p>PDFs derived from Ground Truth, Interpolator, ESPCN, SRCNN, Encoded-CNN, Direct-CNN, and STTA precipitation computed based on an analysis of grid cells and temporal intervals across CONUS and its seven distinct subregions.</p>
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<p>Relative frequency (expressed as %) of specific precipitation-related event characteristics: (<b>a</b>) lifetime average size, km<sup>2</sup> (<span class="html-italic">x</span>-axis is in log); (<b>b</b>) lifetime average intensity, mm/3 h; (<b>c</b>) duration in (3 h) increments; and (<b>d</b>) total volume, m<sup>3</sup> (<span class="html-italic">x</span>-axis is in logarithmic scale), during the life of the event.</p>
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28 pages, 30907 KiB  
Article
Local Sustainability Assessment of the Wonogiri Multipurpose Reservoir Catchment Area in Central Java Province, Indonesia
by Bunga Ludmila Rendrarpoetri, Ernan Rustadi, Akhmad Fauzi and Andrea Emma Pravitasari
Land 2024, 13(11), 1938; https://doi.org/10.3390/land13111938 - 17 Nov 2024
Viewed by 339
Abstract
The sustainability of watershed management is a crucial issue that must be addressed to guarantee the persistence of watershed services including agriculture, food production, and energy supply. This issue has also been addressed in Presidential Regulation No. 18/2020 concerning the National Medium-Term Development [...] Read more.
The sustainability of watershed management is a crucial issue that must be addressed to guarantee the persistence of watershed services including agriculture, food production, and energy supply. This issue has also been addressed in Presidential Regulation No. 18/2020 concerning the National Medium-Term Development Plans for 2020–2024, which stipulate the restoration of priority watersheds, including the Upstream Bengawan Solo Watershed. This study seeks to address this information gap by assessing the local sustainability of the watershed from a temporal dynamics perspective by calculating the Local Sustainability Index (LSI), Local Moran Index, and spatial associations. Measuring sustainable development indices locally is essential because each location has different characteristics, and using specific indicators at the local level is rarely done. The enactment of the national law on village autonomy in Indonesia necessitates the formulation of sustainable development indicators at the village level. These indicators serve as the metrics and frameworks for local government policies and initiatives. Our results show that village sustainability in the social and economic dimensions has increased from 2007 to 2021, especially in urban activity center areas that serve social and economic facilities. This seems different in the environmental dimension, where the sustainability value decreased from 2007 to 2021. The concentration of low sustainability values on ecological conditions occurred in pocket areas. Environmental problems were indicated by land-use conversion and disaster areas. Full article
(This article belongs to the Section Land Environmental and Policy Impact Assessment)
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<p>(<b>a</b>) Map of the Bengawan Solo Watershed area oriented towards Indonesia, (<b>b</b>) map of Upper Bengawan Solo Watershed oriented towards the Bengawan Solo Watershed, (<b>c</b>) sloping map (<b>d</b>) village analysis unit map.</p>
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<p>(<b>a</b>) Map of the Bengawan Solo Watershed area oriented towards Indonesia, (<b>b</b>) map of Upper Bengawan Solo Watershed oriented towards the Bengawan Solo Watershed, (<b>c</b>) sloping map (<b>d</b>) village analysis unit map.</p>
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<p>(<b>a</b>) Map of the Bengawan Solo Watershed area oriented towards Indonesia, (<b>b</b>) map of Upper Bengawan Solo Watershed oriented towards the Bengawan Solo Watershed, (<b>c</b>) sloping map (<b>d</b>) village analysis unit map.</p>
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<p>(<b>a</b>) LSI of social dimension in 2007 and 2021, (<b>b</b>) LSI of economic dimension in 2007 and 2021, (<b>c</b>) LSI environmental dimension in 2007 and 2021.</p>
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<p>(<b>a</b>) LSI of social dimension in 2007 and 2021, (<b>b</b>) LSI of economic dimension in 2007 and 2021, (<b>c</b>) LSI environmental dimension in 2007 and 2021.</p>
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<p>(<b>a</b>) Spatial association of social dimension in 2007 and 2021, (<b>b</b>) Spatial association of economic dimension in 2007 and 2021, (<b>c</b>) Spatial association environmental dimension in 2007 and 2021.</p>
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<p>(<b>a</b>) Combined Category Map of Social Dimension, (<b>b</b>) Combined Category Map of Economic Dimension, (<b>c</b>) Combined Category Map of Environmental Dimension.</p>
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<p>(<b>a</b>) Combined Category Map of Social Dimension, (<b>b</b>) Combined Category Map of Economic Dimension, (<b>c</b>) Combined Category Map of Environmental Dimension.</p>
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<p>(<b>a</b>) Combined Category Map of Social Dimension, (<b>b</b>) Combined Category Map of Economic Dimension, (<b>c</b>) Combined Category Map of Environmental Dimension.</p>
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<p>(<b>a</b>) Combined Category Map of Social Dimension, (<b>b</b>) Combined Category Map of Economic Dimension, (<b>c</b>) Combined Category Map of Environmental Dimension.</p>
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<p>(<b>a</b>) Combined Category Map of Social Dimension, (<b>b</b>) Combined Category Map of Economic Dimension, (<b>c</b>) Combined Category Map of Environmental Dimension.</p>
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<p>(<b>a</b>) Combined Category Map of Social Dimension, (<b>b</b>) Combined Category Map of Economic Dimension, (<b>c</b>) Combined Category Map of Environmental Dimension.</p>
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<p>(<b>a</b>) Combined Category Map of Social Dimension, (<b>b</b>) Combined Category Map of Economic Dimension, (<b>c</b>) Combined Category Map of Environmental Dimension.</p>
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<p>(<b>a</b>) Combined Category Map of Social Dimension, (<b>b</b>) Combined Category Map of Economic Dimension, (<b>c</b>) Combined Category Map of Environmental Dimension.</p>
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<p>(<b>a</b>) Combined Category Map of Social Dimension, (<b>b</b>) Combined Category Map of Economic Dimension, (<b>c</b>) Combined Category Map of Environmental Dimension.</p>
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<p>(<b>a</b>) Combined Category Map of Social Dimension, (<b>b</b>) Combined Category Map of Economic Dimension, (<b>c</b>) Combined Category Map of Environmental Dimension.</p>
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29 pages, 27816 KiB  
Article
Trajectory Aware Deep Reinforcement Learning Navigation Using Multichannel Cost Maps
by Tareq A. Fahmy, Omar M. Shehata and Shady A. Maged
Robotics 2024, 13(11), 166; https://doi.org/10.3390/robotics13110166 - 17 Nov 2024
Viewed by 322
Abstract
Deep reinforcement learning (DRL)-based navigation in an environment with dynamic obstacles is a challenging task due to the partially observable nature of the problem. While DRL algorithms are built around the Markov property (assumption that all the necessary information for making a decision [...] Read more.
Deep reinforcement learning (DRL)-based navigation in an environment with dynamic obstacles is a challenging task due to the partially observable nature of the problem. While DRL algorithms are built around the Markov property (assumption that all the necessary information for making a decision is contained in a single observation of the current state) for structuring the learning process; the partially observable Markov property in the DRL navigation problem is significantly amplified when dealing with dynamic obstacles. A single observation or measurement of the environment is often insufficient for capturing the dynamic behavior of obstacles, thereby hindering the agent’s decision-making. This study addresses this challenge by using an environment-specific heuristic approach to augment the dynamic obstacles’ temporal information in observation to guide the agent’s decision-making. We proposed Multichannel Cost Map Observation for Spatial and Temporal Information (M-COST) to mitigate these limitations. Our results show that the M-COST approach more than doubles the convergence rate in concentrated tunnel situations, where successful navigation is only possible if the agent learns to avoid dynamic obstacles. Additionally, navigation efficiency improved by 35% in tunnel scenarios and by 12% in dense-environment navigation compared to standard methods that rely on raw sensor data or frame stacking. Full article
(This article belongs to the Section AI in Robotics)
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<p>M-COST observation approach. This Diagram illustrates the overall architecture of the proposed technique, detailing each module and the sequential process flow.</p>
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<p>This is an example view from the simulated dynamic environment, where the blue lines represent the rays of the robot’s 2D LIDAR. The white obstacles represent the dynamic obstacles, and the grey obstacles represent the static obstacles, which are randomized in each episode.</p>
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<p>Example of the obstacle cost map with inflation corresponding to the view shown in <a href="#robotics-13-00166-f001" class="html-fig">Figure 1</a>. The blue cells represent cost, the magenta regions represent the highest cost, and the green polygon represents the robot. The coordinates on top of the obstacles represent the tracked dynamic obstacles. Both the robot’s green polygon and the coordinates of the dynamic obstacles are for demonstration purposes only and are not included in the agent’s observations.</p>
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<p>(<b>a</b>) Obstacle trajectory cost map channel, where the robot is marked as a green polygon. The predicted dynamic obstacle trajectories are shown, where the magenta area represents a higher probability distribution of the dynamic obstacles’ future positions in the next time steps, and the blue represents a lower probability for the positions that the dynamic obstacles might take in the near future. (<b>b</b>) A 3D representation of Figure (<b>a</b>) where the <span class="html-italic">z</span>-axis represents the probability of the dynamic obstacles’ position in the next time steps (lighter colors represent higher probabilities).</p>
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<p>Gazebo tunnel simulation environment: Boxes represent obstacles that can be static or dynamic, moving at varying speeds from one side to the other. The humanoids are dynamic actors that also move at varying speeds from one side to the other.</p>
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<p>(<b>a</b>) Gazebo simulation for point-to-point navigation: white obstacles are dynamic, grey obstacles represent static randomized obstacles, and blue rays represent the LIDAR rays. (<b>b</b>) The complex environment with 12 dynamic actors.</p>
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<p>SAC learning process.</p>
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<p>Fully connected neural network architecture of the policy.</p>
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<p>Policy architecture uses CNN as a feature extractor for the cost map and FNN for the extracted features and other scalar data.</p>
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<p>(<b>a</b>) Obstacle cost map as observation, (<b>b</b>) Stacked previous cost maps, and (<b>c</b>) M-COST observation of two channels: obstacles cost map channel and predicted trajectory of the dynamic obstacles in the temporal channel.</p>
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<p>Training reward vs. training steps in the tunnel scenario. Solid lines represent mean training reward, while shaded areas indicate variation or distribution of scores over different steps.</p>
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<p>Evaluation scores vs. training steps in the tunnel scenario. Solid lines represent mean evaluation scores, while shaded areas indicate variation or distribution of scores over different steps.</p>
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<p>Navigation performance metrics for the tunnel environment.</p>
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<p>Training reward vs. training steps in the point-to-point simple scenario. Solid lines represent mean training reward, while shaded areas indicate score distributions over different steps.</p>
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<p>Evaluation scores vs. training steps in the point-to-point simple scenario. Solid lines represent mean evaluation scores, while shaded areas indicate score distributions over different steps.</p>
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<p>Training reward vs. training steps in the point-to-point complex scenario. Solid lines represent mean training reward, while shaded areas indicate score distributions over different steps.</p>
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<p>Evaluation scores vs. training steps in the point-to-point complex scenario. Solid lines represent mean evaluation scores, while shaded areas indicate score distributions over different steps.</p>
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<p>Navigation performance metrics for the point-to-point navigation test with 12 dynamic actors and complex structure environment.</p>
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18 pages, 3819 KiB  
Article
Spatial–Temporal Patterns and the Driving Mechanism for the Gross Ecosystem Product of Wetlands in the Middle Reaches of the Yellow River
by Bi Zhang, Aiping Pang and Chunhui Li
Water 2024, 16(22), 3302; https://doi.org/10.3390/w16223302 - 17 Nov 2024
Viewed by 339
Abstract
Wetlands are crucial for sustainable development, and the evaluation of their GEP is a key focus for governments and scientists. This study created a dynamic accounting model for wetland GEP and assessed the GEP of 39 wetlands in the middle reaches of the [...] Read more.
Wetlands are crucial for sustainable development, and the evaluation of their GEP is a key focus for governments and scientists. This study created a dynamic accounting model for wetland GEP and assessed the GEP of 39 wetlands in the middle reaches of the Yellow River in Ningxia province. The results indicate that Ningxia province’s wetlands have an average annual GEP of CNY 5.24 billion. Haba wetland contributes the most at 0.52, while Qingtongxia, Sha, and Tenggeli wetlands follow with 0.12, 0.04, and 0.03, respectively. Climate regulation is the most valuable function at 38.24%, with species conservation and scientific research/tourism at 24.93% and 15.11%, respectively. Ningxia’s northern wetlands are vast and shaped by the Yellow River, while the smaller, seasonal southern wetlands are more affected by rainfall and mountain groundwater. Southern wetlands show a strong correlation between GEP and precipitation (0.82), whereas northern wetlands have a moderate correlation between GEP and evapotranspiration (0.52). The effective conservation and management of these wetlands require consideration of their locations and weather patterns, along with customized strategies. To maintain the stability of wetland habitats and provide a suitable environment for various species, it is essential to preserve wetlands within a certain size range. Our study found a strong correlation of 0.85 between the wetland area and the GEP value, indicating that the size of wetlands is a key factor in conserving their GEP. The results provide accurate insights for creating a wetland ecological benefit compensation mechanism. Full article
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<p>General dynamic wetland GEP accounting model.</p>
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<p>Location and scope of wetland in Ningxia province.</p>
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<p>Total GEP and GEP proportion of different wetlands.</p>
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<p>The area and average GEP values for different wetlands (to more effectively highlight their distinctions, the values of Sha, Haba, Qingtongxia, and Dangjiacha are displayed in their actual figures).</p>
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<p>Variation trend and composition of wetland GEP from 2000 to 2019.</p>
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<p>Effects of precipitation and evapotranspiration on GEP.</p>
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<p>Relationship between wetland GEP and area.</p>
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19 pages, 8056 KiB  
Article
Ecosystem Stability in the Ugan–Kuqa River Basin, Xinjiang, China: Investigation of Spatial and Temporal Dynamics and Driving Forces
by Ting Zhou, Peiyue Zhu, Rongjin Yang, Yilin Sun, Meiying Sun, Le Zhang and Xiuhong Li
Remote Sens. 2024, 16(22), 4272; https://doi.org/10.3390/rs16224272 - 16 Nov 2024
Viewed by 279
Abstract
Ecosystem stability plays a pivotal role in safeguarding the enduring well-being of both the natural world and human society. This work explores the uncertainty surrounding changes in ecosystem stability and their response mechanisms at localized scales, focusing on the Ugan–Kuqa River Basin in [...] Read more.
Ecosystem stability plays a pivotal role in safeguarding the enduring well-being of both the natural world and human society. This work explores the uncertainty surrounding changes in ecosystem stability and their response mechanisms at localized scales, focusing on the Ugan–Kuqa River Basin in Xinjiang, China. Based on remote sensing data and spatial lag modeling (SLM), we evaluated the spatial and temporal dynamics of the basin’s stability from 2001 to 2020. Additionally, structural equation modeling (SEM) was employed to assess the impacts of climate conditions, human activities, and habitat fragmentation on ecosystem stability. The results of the study indicated that the basin ecosystem stability tended to increase in the temporal dimension, and that the spatial distribution was greater in the north than in the south. In addition, the trade-off between resistance and recovery in the watershed decreased, with a considerable increase in high-resistance–high-recovery zones. Climate warming and increased humidity have emerged as the predominant factors driving the watershed ecosystem stability. Full article
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<p>Schematic diagram of the research region. (<b>a</b>) Geographical location of the study area; (<b>b</b>) Remote sensing image map of the study area; (<b>c</b>) Land use types in the study area; (<b>d</b>) Area percentage of different land use types in the study area.</p>
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<p>Conceptual meta-model of the ecological stability drivers. (<b>a</b>) The overall relationship between stability and climate, vegetation, human activities, and fragmentation. (<b>b</b>) The specific modeling relationship between the drivers of ecosystem stability. The pathways illustrate the interconnections among Pre, Tem, ET, LUCC, GDP, NDVI, ED, PD, LSI, and ecosystem resistance and recovery. Unidirectional arrows denote causation.</p>
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<p>Temporal changes in resistance and recovery.</p>
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<p>Spatial trends in ecosystem resistance and recovery. (<b>a</b>) Spatial trend in resistance. (<b>b</b>) Spatial trend in recovery.</p>
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<p>Scatter plot of resistance–recovery trade-off distributions. The data for both resistance and recovery were standardized within the range of 0 to 1, with the intersection where both factors equate to 0 signifying the origin of the coordinates. The delineation was set at 0.5; values below 0.5 for both resistance and recovery indicate a state of low resistance–low recovery, whereas values surpassing 0.5 indicate high resistance–high recovery. Additionally, resistance above 0.5 and recovery below 0.5 indicate high resistance–low recovery, whereas resistance below 0.5 and recovery above 0.5 suggest low resistance–high recovery.</p>
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<p>Drivers of ecosystem stability, illustrating the simulated effects of climate, vegetation, human ac-tivities, and habitat fragmentation on ecosystem stability. The colors of the arrows represent the degree of significance of the relationships, with dashed arrows indicating nonsignificant correlations. Climatic factors are denoted by blue boxes, vegetation variables are denoted by green boxes, human activity variables are denoted by pink boxes, habitat fragmentation variables are denoted by gray boxes, and stability variables are denoted by yellow boxes.</p>
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<p>Impact of drivers on ecosystem stability. (<b>a</b>) Presents a stacked plot showing the proportions of the total effects of these factors on resistance and recovery, with cells representing negative impacts. (<b>b</b>) Showcases a stacked plot illustrating the percentages of direct versus indirect influences of the variables on resistance, with cells denoting negative impacts. Finally, (<b>c</b>) presents a stacked plot displaying the percentages of direct versus indirect influences of the variables on recovery, with cells indicating a negative impact.</p>
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