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22 pages, 1716 KiB  
Article
Community Assembly Mechanisms of Populus euphratica in Northwest China and Their Relationship with Environmental Factors
by Lijun Zhu, Jie Wang, Houji Liu, Juntuan Zhai and Zhijun Li
Plants 2024, 13(23), 3283; https://doi.org/10.3390/plants13233283 (registering DOI) - 22 Nov 2024
Abstract
Populus euphratica is a key community-building species in the desert riparian forests of Northwest China, exhibiting exceptional resistance to stress and playing a vital role in soil and water conservation as well as maintaining ecological balance in arid regions. To investigate the ecological [...] Read more.
Populus euphratica is a key community-building species in the desert riparian forests of Northwest China, exhibiting exceptional resistance to stress and playing a vital role in soil and water conservation as well as maintaining ecological balance in arid regions. To investigate the ecological processes underlying the composition of P. euphratica communities and to identify their community construction mechanisms, this study analyses the species diversity and phylogenetic diversity of 58 P. euphratica communities, exploring their assembly processes and key influencing factors. This research aims to elucidate the relationship between community structure from the perspective of species evolution and analyse the construction mechanisms of P. euphratica communities across different clusters in arid environments. The results show that the species diversity of P. euphratica clusters in Northwest China is relatively low, and a significant correlation is noted with phylogenetic diversity (PD). The Shannon–Wiener and Margalef indices exhibit similar trends, whereas Simpson’s index show the opposite trends. Pielou’s index range from 0.7 to 0.85. Notably, the PD and species diversity of the P. euphratica–Haloxylon ammodendron association group (Group 4) is significantly higher (p < 0.05) compared to that of the other groups. Additionally, net relatedness index (NRI) and nearest taxon index (NTI) peaked in the P. euphratica–H. ammodendron association group (Group 4) and the Populus pruinosa–Tamarix ramosissima–Phragmites australis association group (Group 1) (p < 0.05). A Pearson correlation analysis indicated that PD was significantly positively correlated with Margalef’s index, Shannon–Wiener’s index, and Pielou’s index, but was significantly negatively correlated with Simpson’s index, while also being associated with environmental factors. Key factors influencing the diversity of P. euphratica communities in Northwest China include total phosphorus, pH, soil moisture content, total potassium, the mean temperature of the coldest quarter, precipitation of the wettest month, and precipitation seasonality. Soil factors primarily affected the Pielou and Simpson indices of species diversity, whereas climatic factors mainly influenced the Margalef and Shannon–Wiener indices. PD and structure were mainly influenced by climatic factors. The combined effects of soil and climatic factors play a crucial role in sustaining the diversity and ecological adaptation of these plant communities. In summary, P. euphratica communities may exhibit a significant ecological niche conservation in response to environmental changes, and competitive exclusion might be the primary process shaping community structure. Climatic factors were shown to be important regulators of community diversity and phylogenetic structure. Full article
(This article belongs to the Section Plant Ecology)
20 pages, 3128 KiB  
Article
Straw Returning Methods Affects Macro-Aggregate Content and Organic Matter Content in Black Soils: Meta-Analysis and Comprehensive Validation
by Kangmeng Liu, Yu Hu, Yumei Li, Lei Wang, Liang Jin, Lianfeng Cai, Xiaoxiao Wu, Zhenguo Yang, Yan Li and Dan Wei
Plants 2024, 13(23), 3284; https://doi.org/10.3390/plants13233284 (registering DOI) - 22 Nov 2024
Abstract
Straw returning into the soil is a crucial method for boosting soil carbon levels. To research the influence of straw return practices on soil aggregates and organic matter content within the farmlands of the Northeast Black Soil Region, the objective was to clarify [...] Read more.
Straw returning into the soil is a crucial method for boosting soil carbon levels. To research the influence of straw return practices on soil aggregates and organic matter content within the farmlands of the Northeast Black Soil Region, the objective was to clarify the varying impacts of these practices on soil carbon enhancement. In this study, 89 pertinent papers were acquired through a rigorous literature compilation. Meta-analysis and the linear regression method were employed to analyze the influence of field return methods, their duration on soil water-stable aggregates, and their organic matter content. Furthermore, the study delved into the trends in the variation of aggregates and organic matter in relation to mean annual temperature and precipitation. Our results showed that the straw-returning method has been discovered to predominantly bolster soil organic matter by altering the proportions of macro-aggregate content. Specifically, straw incorporation has led to a notable enhancement in the content of macro-aggregates (57.14%) and micro-aggregates (20.29%), in addition to augmenting the content of macro-, small, and micro-aggregate organic matter by 13.22%, 16.43%, and 15.08%, respectively. The most significant increase in large agglomerates was witnessed in straw return over a period of more than 5 years (115.17%), as well as shallow mixing return (87.32%). Meanwhile, the highest increase in the organic matter content of large agglomerates was recorded in straw return over 5 years (12.60%) and deep mixing return (8.72%). In the field validation experiment, a period of seven years of straw return significantly boosted the macro-aggregate content across various soil layers, ranging from 11.78% to 116.21%. Furthermore, among the various climatic factors, the primary determinants of disparities in study outcomes were the average annual temperature and average annual precipitation. Specifically, lower precipitation and higher temperatures were conducive to the enhancement of macro-aggregate formation and organic matter content. Full article
(This article belongs to the Special Issue Water and Nitrogen Management in the Soil–Crop System (3rd Edition))
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<p>Effects of straw returning to field on water-stable aggregate (<b>A</b>) and organic matter (<b>B</b>) content.</p>
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<p>Effect of straw returning method (NTS, STS, and DTS) and age (&lt;3 years, 3–5 years and &gt;5 years) on water-stable aggregate contents in different soil layers (A and B). NTS, mulching return; STS: shallow-mixed return; DTS, deep-mixed return; &lt;3 years, short-term; 3–5 years, medium-term; &gt;5 years, long-term; A, 0–20 cm; B, 20–40 cm.</p>
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<p>Relationship between water-stable aggregate content (<b>A</b>), organic matter content of aggregates (<b>B</b>), and straw returning years. The green dots represent response ratio of water-stable aggregate contents observations. The 95% confidence interval is indicated by a light brown shaded.</p>
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<p>Effects of straw returning methods (NTS, STS and DTS) and years (&lt;3 years, 3–5 years, and &gt;5 years) on the content of organic matter in water-stable aggregates in different soil layers (A and B). NTS, mulching return; STS: shallow-mixed return; DTS, deep-mixed return; &lt;3 years, short-term; 3–5 years, medium-term; &gt;5 years, long-term; A, 0–20 cm; B, 20–40 cm.</p>
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<p>The relationship between water-stable aggregates (<b>A</b>,<b>B</b>), organic matter content (<b>C</b>,<b>D</b>), average annual precipitation (MAP), and average annual temperature (MAT).</p>
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<p>The relationship between the response ratio of aggregate and organic matter content.</p>
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<p>Effects of returning years (1 year, 4 years, and 7 years) and methods (NT, NTS, STS, and DTS) on soil water-stable aggregate (LA, MA and SA) content. NT, no tillage with straw removed from the field; NTS, no tillage with 100% straw retention; STS, tilling to a depth of 20 cm in autumn with 100% straw retention; DTS, tilling to a depth of 35 cm in autumn with 100% straw retention; LA, &gt;2 mm; MA, 0.25–2 mm; SA, &lt;0.25 mm.</p>
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<p>Flow diagram of database screening.</p>
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17 pages, 5372 KiB  
Article
Ecological Importance Evaluation and Ecological Function Zoning of Yanshan-Taihang Mountain Area of Hebei Province
by Pengtao Zhang, Qixuan Duan, Jie Dong, Lichao Piao and Zhaoyang Cui
Sustainability 2024, 16(23), 10233; https://doi.org/10.3390/su162310233 (registering DOI) - 22 Nov 2024
Abstract
Ecological importance evaluation can clearly identify the ecological service functions and ecological values of a region. This paper takes the Yanshan-Taihang Mountain area in Hebei Province as the research area, utilizing 2020 land use data. With the help of various analytical models and [...] Read more.
Ecological importance evaluation can clearly identify the ecological service functions and ecological values of a region. This paper takes the Yanshan-Taihang Mountain area in Hebei Province as the research area, utilizing 2020 land use data. With the help of various analytical models and GIS spatial analysis methods, this paper selects water conservation, soil and water conservation, biodiversity, carbon sequestration and oxygen release to evaluate the importance of ecosystem services, and selects soil and water loss sensitivity and land desertification sensitivity to evaluate the ecological sensitivity, so as to identify the important areas of ecological protection in the study area, analyze their spatial change characteristics and divide the leading ecological functions according to the results. The results show that the moderately important and highly important areas in the Yanshan-Taihang region of Hebei Province account for more than 70% of the total study area. Based on the importance evaluation results, three types of dominant ecological function zones were obtained using self-organized feature mapping neural network analysis in the R language, and control measures were proposed. The research results can provide strategic support for local ecological protection and regional ecological restoration, as well as serving as a reference for the optimization of land spatial development patterns. Full article
(This article belongs to the Section Soil Conservation and Sustainability)
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<p>Overview of the study area.</p>
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<p>Group diagram of ecosystem service evaluation results. In the figure, (<b>a</b>) represents the evaluation result of water source conservation; (<b>b</b>) is the evaluation result of carbon fixation and oxygen release; (<b>c</b>) is the result of soil conservation evaluation; (<b>d</b>) is the evaluation result of biodiversity conservation and (<b>e</b>) shows the evaluation results of the importance of ecosystem service functions.</p>
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<p>Group map of ecological sensitivity evaluation results. In the figure, (<b>a</b>) represents the evaluation result of soil erosion; (<b>b</b>) is the evaluation result of land desertification; (<b>c</b>) is the result of ecological sensitivity assessment.</p>
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<p>Results of ecological importance evaluation.</p>
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<p>R language clustering output.</p>
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<p>Partition result diagram.</p>
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27 pages, 2666 KiB  
Review
Farming Practice Variability and Its Implications for Soil Health in Agriculture: A Review
by Elsadig Omer, Dora Szlatenyi, Sándor Csenki, Jomana Alrwashdeh, Ivan Czako and Vince Láng
Agriculture 2024, 14(12), 2114; https://doi.org/10.3390/agriculture14122114 (registering DOI) - 22 Nov 2024
Abstract
Soil health is essential for sustainable agricultural operations, as it supports farm production and ecosystem services. The adoption of sustainable agriculture practices such as conservation tillage, cover cropping, and crop rotation provides significant benefits for both crop productivity and environmental sustainability. These practices [...] Read more.
Soil health is essential for sustainable agricultural operations, as it supports farm production and ecosystem services. The adoption of sustainable agriculture practices such as conservation tillage, cover cropping, and crop rotation provides significant benefits for both crop productivity and environmental sustainability. These practices can increase soil biodiversity, nutrient cycling, and organic matter, which increase the resilience of agroecosystems. This narrative review synthesizes the insights of the soil health practices adoption literature, with a focus on common farming practices that can improve soil health and enhance crop yields, reviewing the results of various approaches and pointing out the challenges and opportunities for implementing sustainable agriculture on a larger scale. This paper discusses the effects of various tillage and cropping system approaches on soil health, including no-till and conventional tillage systems, crop rotation, cover cropping, cultivator combinations, and fertilizer application. This study found that conservation tillage is more beneficial to soil health than conventional tillage—which is still debated among scientists and farmers—and that different tillage methods interact differently. In contrast, agricultural yields increase more with intercropping, crop rotation, and cover crops than monocropping. For maintaining soil fertility, this study shows that agricultural yields could be increased by implementing zero tillage. This review identifies the most suitable farming practices for improving soil health while boosting crop production with minimal negative impact on the soil. It also highlights the benefits of these practices in maintaining soil quality. Full article
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<p>Principles of soil health recommended by USDA-NRCS.</p>
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<p>Linking soil health to ecosystem services.</p>
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<p>Soil management techniques or control measures for sustainable agriculture.</p>
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<p>Optimal physical, biological, and chemical properties promote soil health.</p>
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<p>Pumping and cycling of nutrients through the building of a “safety net” by enhancing organic matter inputs, accessing deep soil nutrients, and improving soil structure, through carful management is required to balance resource competition in agroforestry system, by [<a href="#B189-agriculture-14-02114" class="html-bibr">189</a>].</p>
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<p>Knowledge gaps exist in our current understanding of soil health.</p>
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27 pages, 8809 KiB  
Article
Trend Analysis of High-Resolution Soil Moisture Data Based on GAN in the Three-River-Source Region During the 21st Century
by Zhuoqun Li, Siqiong Luo, Xiaoqing Tan and Jingyuan Wang
Remote Sens. 2024, 16(23), 4367; https://doi.org/10.3390/rs16234367 (registering DOI) - 22 Nov 2024
Abstract
Soil moisture (SM) is a crucial factor in land-atmosphere interactions and climate systems, affecting surface energy, water budgets, and weather extremes. In the Three-River-Source Region (TRSR) of China, rapid climate change necessitates precise SM monitoring. This study employs a novel UNet-Gan model to [...] Read more.
Soil moisture (SM) is a crucial factor in land-atmosphere interactions and climate systems, affecting surface energy, water budgets, and weather extremes. In the Three-River-Source Region (TRSR) of China, rapid climate change necessitates precise SM monitoring. This study employs a novel UNet-Gan model to integrate and downscale SM data from 17 CMIP6 models, producing a high-resolution (0.1°) dataset called CMIP6UNet-Gan. This dataset includes SM data for five depth layers (0–10 cm, 10–30 cm, 30–50 cm, 50–80 cm, 80–110 cm), four Shared Socioeconomic Pathways (SSP1-2.6, SSP2-4.5, SSP3-7.0, SSP5-8.5). The UNet-Gan model demonstrates strong performance in data fusion and downscaling, especially in shallow soil layers. Analysis of the CMIP6UNet-Gan dataset reveals an overall increasing trend in SM across all layers, with higher rates under more intense emission scenarios. Spatially, moisture increases vary, with significant trends in the western Yangtze and northeastern Yellow River regions. Deeper soils show a slower response to climate change, and seasonal variations indicate that moisture increases are most pronounced in spring and winter, followed by autumn, with the least increase observed in summer. Future projections suggest higher moisture increase rates in the early and late 21st century compared to the mid-century. By the end of this century (2071–2100), compared to the Historical period (1995–2014), the increase in SM across the five depth layers ranges from: 5.5% to 11.5%, 4.6% to 9.2%, 4.3% to 7.5%, 4.5% to 7.5%, and 3.3% to 6.5%, respectively. Full article
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<p><b>Above</b> is the location of the study area in China, <b>below</b> is the elevation of the study area and the location of the in-site.</p>
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<p>Training details of the UNet-GAN network.</p>
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<p>The model structure of U-Net (with three output channels for the pretraining phase and five output channels for the fine-tuning phase).</p>
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<p>The model structure of the discriminator. The number following <span class="html-italic">k</span> indicates the convolution kernel size, the number following <span class="html-italic">n</span> indicates the number of output channels, and the number following <span class="html-italic">s</span> indicates the stride.</p>
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<p>Box plots of evaluation metrics for SM from different layers of ERA5-Land and AMSMQTP compared to in situ observations (for MAE, RMSE, and MAPE, smaller values are better, and for R, larger values are better).</p>
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<p>Scatter plot of predicted values versus true values on the test set for five-fold cross-validation (The red dotted line represents a 45-degree diagonal, and the color intensity indicates the density of the points).</p>
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<p>A comparison of the data predicted by the UNet-Gan model for the first layer in the test set with the data from 17 CMIP6 models and their Ensemble, against the AMSMQTP data. The diagram includes three metrics: RMSE, standard deviation, and correlation coefficient.</p>
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<p>Spatial distribution of the annual mean SM trends for Layer 1 under four emission scenarios, with dotted areas indicating regions significant at the 95% confidence level.</p>
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<p>Spatial distribution of annual average SM trend changes in Layer 1 under four emission scenarios for different seasons, with highlighted areas indicating significance at the 95% confidence level (DJF represents winter, MAM represents spring, JJA represents summer, and SON represents autumn).</p>
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<p>Time series of annual average SM for Layer 1 under different historical and future emission scenarios. The legend provides the rate of change, with units in m<sup>3</sup>/m<sup>3</sup> per year, and asterisks indicate significance at the 95% confidence level.</p>
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<p>Time series of annual average SM for Layer 1 under historical and future emission scenarios in different seasons, with the legend showing the rate of change in units of m<sup>3</sup>/m<sup>3</sup> per year. Asterisks indicate significance at the 95% confidence level (DJF represents winter, MAM represents spring, JJA represents summer, and SON represents autumn).</p>
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<p>Time series of annual average SM for Layer 1 under historical and future emission scenarios in different seasons, with the legend showing the rate of change in units of m<sup>3</sup>/m<sup>3</sup> per year. Asterisks indicate significance at the 95% confidence level (DJF represents winter, MAM represents spring, JJA represents summer, and SON represents autumn).</p>
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<p>Same as <a href="#remotesensing-16-04367-f011" class="html-fig">Figure 11</a>, but for JJA and SON.</p>
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<p>Same as <a href="#remotesensing-16-04367-f011" class="html-fig">Figure 11</a>, but for JJA and SON.</p>
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<p>The mean SM of the five layers in TRSR over different periods, with the Historical period covering 1995–2014, and the four emission scenarios covering 2021–2040, 2041–2070, and 2071–2100, respectively. MAM, JJA, SON, and DJF represent mean SM values for spring, summer, autumn, and winter, respectively, while Y represents the mean annual SM.</p>
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<p>The spatial distribution of the percentage increase in Layer 1 SM under four emission scenarios for the end of the 21st century (2071–2100) compared to the beginning of the century (1995–2014).</p>
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<p>The rate of change in SM for the five layers in TRSR over different periods, with the Historical period covering 1995–2014, and the four emission scenarios covering 2021–2040, 2041–2070, and 2071–2100, respectively. Rates that did not pass the 95% significance test are not shown in the figure. MAM, JJA, SON, and DJF represent the rate of SM change for spring, summer, autumn, and winter, respectively, while Y represents the annual rate of SM change, with units in m<sup>3</sup>/m<sup>3</sup> per year.</p>
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16 pages, 1955 KiB  
Article
Adaptive Recognition and Control of Shield Tunneling Machine in Soil Layers Containing Plastic Drainage Boards
by Qiuping Wang, Wanli Li, Zhikuan Xu and Yougang Sun
Actuators 2024, 13(12), 470; https://doi.org/10.3390/act13120470 - 22 Nov 2024
Abstract
The underground plastic vertical drains (PVDs) are a significant problem for shield machines in tunneling construction. At present, the main method to deal with PVDs is to manually adjust the parameters of the shield machine. To ensure that a shield machine autonomously recognizes [...] Read more.
The underground plastic vertical drains (PVDs) are a significant problem for shield machines in tunneling construction. At present, the main method to deal with PVDs is to manually adjust the parameters of the shield machine. To ensure that a shield machine autonomously recognizes and adjusts the control in soil layers containing PVDs, this study constructs a shield machine advance and rotation state-space model utilizing Bayesian decision theory for the judgment of excavation conditions. A Bayesian model predictive control (Bayes-MPC) method for the shield machine is proposed, followed by a simulation analysis. Finally, a validation experiment is conducted based on a Singapore subway project. Compared with traditional methods, the method proposed in this paper has better performance in the simulation, and it also has demonstrated effectiveness and accuracy in experiments. The research outcomes can provide a reference for the adaptive assistance system of shield machines excavating underground obstacles. Full article
(This article belongs to the Section Actuators for Land Transport)
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<p>(<b>a</b>) Distribution of plastic drainage plates in a tunnel project; (<b>b</b>) longitudinal geological section schematic.</p>
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<p>Advance and rotation system of shield machine.</p>
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<p>Simplified control model for a single hydraulic cylinder.</p>
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<p>Bayes-MPC method for shield machine.</p>
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<p>Simulation outputs.</p>
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<p>Simulation inputs.</p>
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<p>Experimental situation.</p>
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<p>(<b>a</b>) Cutterhead speed control performance; (<b>b</b>) excavation speed control performance.</p>
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<p>(<b>a</b>) Comparison of cutterhead torque; (<b>b</b>) comparison of thrust.</p>
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21 pages, 2854 KiB  
Article
Ecological Restoration Process of El Hito Saline Lagoon: Potential Biodiversity Gain in an Agro-Natural Environment
by Carlos Nuévalos-Tello, Daniel Hernández-Torres, Santiago Sardinero-Roscales, Miriam Pajares-Guerra, Anna Chilton and Raimundo Jiménez-Ballesta
Land 2024, 13(12), 1992; https://doi.org/10.3390/land13121992 - 22 Nov 2024
Abstract
In the global context of biodiversity and ecosystem services loss, the integration of agriculture with ecological restoration is crucial.. This study presents the biodiversity value (Bv) index for the first time as a tool for decision-making and securing funding for future restoration projects. [...] Read more.
In the global context of biodiversity and ecosystem services loss, the integration of agriculture with ecological restoration is crucial.. This study presents the biodiversity value (Bv) index for the first time as a tool for decision-making and securing funding for future restoration projects. The Bv index was used to assess biodiversity values in both restored natural habitats and agricultural areas in the saline lagoon of El Hito, a natural reserve located within an agricultural landscape in central Spain. Additionally, we estimated biodiversity gains from habitat transitions and explored the relationship between biodiversity, soil pH, and salinity. Sustainable agricultural practices, combined with ecological restoration methods, can lead to synergistic actions that reduce the potential detrimental effects of agriculture. Our results show that transitioning from agricultural to natural habitats consistently increases biodiversity. Among agricultural practices, multiannual vegetated fallows had the highest Bv values. Restoration led to a continuous biodiversity improvement, with the exception of the final transition from permanent pastures to Elymus 1410, which showed a slight decline in biodiversity. We also found that higher soil salinity and pH were associated with greater biodiversity values, likely due to historical agricultural practices that favored areas with lower salinity and pH for higher productivity. Salinity and pH act as limiting factors for biodiversity; therefore, agricultural plots with lower salinity and pH, particularly those adjacent to natural habitats, are expected to yield greater biodiversity gains if restored. Full article
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<p>Former livestock farm within the El Hito Lagoon Nature Reserve prior to the restoration of the lagoon.</p>
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<p>Habitats distribution and sampling points.</p>
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<p>Sampling methods for (<b>A</b>) pollinators with Blue Vane trap. (<b>B</b>) Aboveground arthropods with BLACK+DECKER BCBLV36B-XJ garden vacuum. (<b>C</b>) Two-square-meter quadrats used to measure flora. (<b>D</b>) Soil bacteria, fungi, and arthropods sampling by a soil auger.</p>
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<p>Histogram representing the total Bv values of each measured habitat.</p>
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<p>Linear regression between electric conductivity and pH.</p>
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<p>Linear regression between total Bv and pH.</p>
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15 pages, 3987 KiB  
Review
Sugarcane Pokkah Boeng Disease: Insights and Future Directions for Effective Management
by Rajendran Poorniammal, Jerald Jernisha, Somasundaram Prabhu and Laurent Dufossé
Life 2024, 14(12), 1533; https://doi.org/10.3390/life14121533 - 22 Nov 2024
Abstract
Pokkah Boeng disease has been observed in nearly all countries where sugarcane is commercially cultivated. The disease was considered a minor concern in earlier times, but due to climate change, it has now become a major issue. It is caused by fungi, specifically [...] Read more.
Pokkah Boeng disease has been observed in nearly all countries where sugarcane is commercially cultivated. The disease was considered a minor concern in earlier times, but due to climate change, it has now become a major issue. It is caused by fungi, specifically the Fusarium fungal complex. Fusarium fujikuroi, F. sacchari, F. oxysporum, F. verticillioides, F. proliferatum, and F. subglutinans are the major species causing the disease in sugarcane. The disease spreads rapidly, and unpredictable environmental conditions, along with the overlap of crop stages with biotic factors, contributed to its increased severity and varied symptom patterns. This disease is primarily airborne, spreading through air currents. Secondary transmission occurs via infected setts, irrigation water, splashed rain, and soil. It typically emerges during hot and humid conditions, particularly when the sugarcane is experiencing rapid growth. The most effective way to control Pokkah Boeng is by cultivating resistant varieties and removing canes exhibiting ‘top rot’ or ‘knife cut’ symptoms. Apply 0.1% carbendazim, 0.2% copper oxychloride, or 0.3% mancozeb for two to three sprayings at 15-day intervals. Using biological methods to control plant pathogens presents a promising alternative to the heavy reliance on chemical fungicides in modern agriculture, which can lead to environmental pollution and the development of resistant strains. Full article
(This article belongs to the Section Plant Science)
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<p>Sugarcane production (MT million tons) from 2019 to 2024 (March) (FAOSTAT, 2024). (<b>a</b>) Worldwide sugarcane production; (<b>b</b>) Indian state wise sugarcane production.</p>
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<p>Morphological and conidial characteristics of <span class="html-italic">Fusarium</span> sp.(<b>a</b>) <span class="html-italic">F. fujikuroi</span> strain CSV1 (<b>b</b>) <span class="html-italic">F. proliferatum</span>, (<b>c</b>) <span class="html-italic">F. fujikuroi</span> strain Augusto 2, and (<b>d</b>) <span class="html-italic">F. oxysporum</span>.</p>
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<p>Pokahh Boeng symptoms on sugarcane (<b>a</b>) dhlorotic phase, (<b>b</b>) top rot phase, (<b>c</b>) knife cut phase, (<b>d</b>) ladder-like lesions on shoot, (<b>e</b>) twisted leafs, and (<b>f</b>) malformed shoot.</p>
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<p>Life cycle of <span class="html-italic">Fusarium</span> in Pokkah Boeng disease.</p>
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<p>Tripartite interaction of Pokka Boeng, mealybug, and sugarcane.</p>
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<p>Mechanism of plant growth promoting rhizobacteria for disease control.</p>
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28 pages, 7925 KiB  
Article
Assessment of Soil Loss Due to Wind Erosion and Dust Deposition: Implications for Sustainable Management in Arid Regions
by Abdulhakim J. Alzahrani, Abdulaziz G. Alghamdi and Hesham M. Ibrahim
Appl. Sci. 2024, 14(23), 10822; https://doi.org/10.3390/app142310822 - 22 Nov 2024
Abstract
Soil loss due to wind erosion and dust deposition has become a growing concern, particularly in arid regions like Al-Baha, Saudi Arabia. The aim of this study was to quantitatively assess soil loss and dust deposition using three different dust collection methods across [...] Read more.
Soil loss due to wind erosion and dust deposition has become a growing concern, particularly in arid regions like Al-Baha, Saudi Arabia. The aim of this study was to quantitatively assess soil loss and dust deposition using three different dust collection methods across 20 sites during the summer of 2022. The methods include Big Spring Number Eight (BSNE), which measures airborne dust particles using passive samplers; Surface Dust Collector (SDC), designed to collect dust settling on the ground surface; and Marble Dust Collector (MDCO), which utilizes marble-coated surfaces to trap and measure dust deposition. These methods collectively provide a comprehensive evaluation of dust dynamics in the study area. The objective was to evaluate the effects of wind erosion and dust deposition on soil properties, offering insights into the mechanisms of soil loss in arid environments. The study revealed significant variations in soil characteristics, including low organic matter content (<1%), high calcite (up to 19.62%), and increased salinity levels, with notable quantities of Cl (211.58 meq kg⁻1) and Na (165.98 meq kg⁻1). July showed the highest dust deposition (0.0133 ton ha−1), particularly at site S11, while soil loss was lowest at site S5. This research offers novel insights into the nonlinear relationship between soil loss and time, contributing to sustainable soil management strategies. By aligning with Saudi Arabia’s Vision 2030 and the Sustainable Development Goals (SDGs), the findings underscore the need to mitigate soil loss to enhance environmental sustainability, prevent desertification, and promote long-term resilience in arid regions. Full article
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<p>Administrative boundaries of Al-Baha region and its affiliated governorates.</p>
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<p>A geological map of Kingdom of Saudi Arabia (KSA) showing a clearer representation of the geological domains and their extent within the Al-Baha region.</p>
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<p>Locations of sample collection from all 20 sites where the dust-sampling methods were employed. Colored lines represent contours in meters, illustrating the topographical variations across the study area.</p>
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<p>Dust-collection instruments and their deposition mechanisms: (<b>A</b>) Big Spring Number Eight (BSNE) Dust Collector, capturing airborne dust at various heights; (<b>B</b>) Surface Dust Collector (SDC), collecting surface dust directly affected by wind; and (<b>C</b>) Marble Dust Collector (MDCO), utilizing marble trays to capture deposited dust.</p>
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<p>The least significant difference (LSD) values at <span class="html-italic">p</span> &lt; 0.05 for the amount of dust (ton h<sup>−1</sup>) collected by the variable dust collector devices at the different sites.</p>
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<p>Mineralogical composition (XRD) of collected soil samples from the studied location (S1 to S20 are the study sites).</p>
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<p>Classification of study area based on contour lines (<b>A</b>), slope degree (<b>B</b>), soil type (<b>C</b>), and stream orders (<b>D</b>). Each panel provides insights into the geographical and environmental characteristics that influence dust-erosion and soil-formation processes in the Al-Baha region.</p>
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<p>Classification of study area based on contour lines (<b>A</b>), slope degree (<b>B</b>), soil type (<b>C</b>), and stream orders (<b>D</b>). Each panel provides insights into the geographical and environmental characteristics that influence dust-erosion and soil-formation processes in the Al-Baha region.</p>
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<p>Soil loss across studied sites (S1–S20) due to the falling dust in terms of wind. Erosion (<b>A</b>), wind surface disturbance (<b>B</b>), and dust deposition (<b>C</b>).</p>
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<p>The sensitivity of various sites in the Al-Baha region to wind erosion (<b>A</b>), wind surface disturbance (<b>B</b>), and dust deposition (<b>C</b>), measured in tons per hectare (ton ha<sup>−1</sup>).</p>
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<p>The sensitivity of various sites in the Al-Baha region to wind erosion (<b>A</b>), wind surface disturbance (<b>B</b>), and dust deposition (<b>C</b>), measured in tons per hectare (ton ha<sup>−1</sup>).</p>
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19 pages, 53371 KiB  
Article
Efficient UAV-Based Automatic Classification of Cassava Fields Using K-Means and Spectral Trend Analysis
by Apinya Boonrang, Pantip Piyatadsananon and Tanakorn Sritarapipat
AgriEngineering 2024, 6(4), 4406-4424; https://doi.org/10.3390/agriengineering6040250 - 22 Nov 2024
Abstract
High-resolution images captured by Unmanned Aerial Vehicles (UAVs) play a vital role in precision agriculture, particularly in evaluating crop health and detecting weeds. However, the detailed pixel information in these images makes classification a time-consuming and resource-intensive process. Despite these challenges, UAV imagery [...] Read more.
High-resolution images captured by Unmanned Aerial Vehicles (UAVs) play a vital role in precision agriculture, particularly in evaluating crop health and detecting weeds. However, the detailed pixel information in these images makes classification a time-consuming and resource-intensive process. Despite these challenges, UAV imagery is increasingly utilized for various agricultural classification tasks. This study introduces an automatic classification method designed to streamline the process, specifically targeting cassava plants, weeds, and soil classification. The approach combines K-means unsupervised classification with spectral trend-based labeling, significantly reducing the need for manual intervention. The method ensures reliable and accurate classification results by leveraging color indices derived from RGB data and applying mean-shift filtering parameters. Key findings reveal that the combination of the blue (B) channel, Visible Atmospherically Resistant Index (VARI), and color index (CI) with filtering parameters, including a spatial radius (sp) = 5 and a color radius (sr) = 10, effectively differentiates soil from vegetation. Notably, using the green (G) channel, excess red (ExR), and excess green (ExG) with filtering parameters (sp = 10, sr = 20) successfully distinguishes cassava from weeds. The classification maps generated by this method achieved high kappa coefficients of 0.96, with accuracy levels comparable to supervised methods like Random Forest classification. This technique offers significant reductions in processing time compared to traditional methods and does not require training data, making it adaptable to different cassava fields captured by various UAV-mounted optical sensors. Ultimately, the proposed classification process minimizes manual intervention by incorporating efficient pre-processing steps into the classification workflow, making it a valuable tool for precision agriculture. Full article
(This article belongs to the Special Issue Computer Vision for Agriculture and Smart Farming)
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<p>Study area of cassava fields captured by the DJI Phantom 4 Pro sensor.</p>
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<p>Study area of cassava fields captured by the DJI Phantom 4 sensor.</p>
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<p>Proposed classification process.</p>
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<p>Boxplot of the spectral value of classes.</p>
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<p>Kappa coefficient of K-means, RF, and the proposed classification process.</p>
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<p>Classification results using the proposed classification process: (<b>a</b>) Plot 1, showing results from an area with patchy weeds and thin weed patches; (<b>b</b>) Plot 5, showing results from an area with fewer weed patches and dense weed coverage; (<b>c</b>) Plot 8, showing results from an area with varying light illumination.</p>
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17 pages, 4157 KiB  
Article
Laccase Production Optimization from Recombinant E. coli BL21 Codon Plus Containing Novel Laccase Gene from Bacillus megaterium for Removal of Wastewater Textile Dye
by Zannara Mustafa, Ikram ul Haq, Ali Nawaz, Abdulrahman H. Alessa, Muhammad Nauman Aftab, Ahmad A. Alsaigh and Aziz ur Rehman
Molecules 2024, 29(23), 5514; https://doi.org/10.3390/molecules29235514 - 22 Nov 2024
Abstract
The aim of the present research was the efficient degradation of industrial textile wastewater dyes using a very active cloned laccase enzyme. For this purpose, potent laccase-producing bacteria were isolated from soil samples collected from wastewater-replenished textile sites in Punjab, Pakistan. The laccase [...] Read more.
The aim of the present research was the efficient degradation of industrial textile wastewater dyes using a very active cloned laccase enzyme. For this purpose, potent laccase-producing bacteria were isolated from soil samples collected from wastewater-replenished textile sites in Punjab, Pakistan. The laccase gene from locally isolated strain LI-81, identified as Bacillus megaterium, was cloned into vector pET21a, which was further transformed into E. coli BL21 codon plus. The optimized conditions for the increased production of laccase include fermentation in a 2% glucose, 5% yeast extract and 250 mg/L CuSO4 medium with pH 7.5; inoculation with 5% inoculum; induction with 0.1 mM IPTG at 0.5 O.D.; and incubation for 36 h at 37 °C. The crude enzyme produced was employed for the removal of commercially used textile dyes. The dyes were quickly precipitated under optimized reaction conditions. Rose bengal, brilliant green, brilliant blue G, Coomassie brilliant blue R and methylene blue were precipitated at rates of 10.69, 54.47, 84.04, 78.99 and 7.40%, respectively. The FTIR and UV–Vis spectroscopic analyses of dyes before and after confirmed the chemical changes brought about by the cloned laccase that led to the dye removal. Full article
(This article belongs to the Section Chemical Biology)
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<p>Brown zone produced by laccase-producing bacteria isolated from soil.</p>
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<p>(<b>a</b>) Colony PCR of laccase gene in vector PCR 2.1 transformed into DH5α. (<b>b</b>) Colony PCR of laccase gene cloned into pET21a further transformed into BL21 codon plus.</p>
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<p>Comparison of rILac activity in different fractions of LB broth culture medium.</p>
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<p>Effect of (<b>A</b>): <b>medium composition</b> (37 °C, 24 h of fermentation, 7 pH, 2% inoculum, 0.1 mM IPTG, 0.4 O.D., 100 mg/L CuSO<sub>4</sub>); (<b>B</b>): <b>temperature</b> (YPD-Cu, 24 h of fermentation, 7 pH, 2% inoculum, 0.1 mM IPTG, 0.4 O.D., 100 mg/L CuSO<sub>4</sub>); (<b>C</b>): <b>fermentation time</b> (37 °C, YPD-Cu, 7 pH, 2% inoculum, 0.1 mM IPTG, 0.4 O.D., 100 mg/L CuSO<sub>4</sub>); (<b>D</b>): <b>pH</b> (37 °C, YPD-Cu, 24 h of fermentation, 2% inoculum, 0.1 mM IPTG, 0.4 O.D., 100 mg/L CuSO<sub>4</sub>); (<b>E</b>): <b>carbon source</b> (37 °C, 24 h of fermentation, 0.2% yeast extract, 0.5% peptone, 2% inoculum, 0.1 mM IPTG, 0.4 O.D., 100 mg/L CuSO<sub>4</sub>); (<b>F</b>): <b>concentration of carbon source</b> (37 °C, YPD-Cu, 24 h of fermentation, 0.2% yeast extract, 0.5% peptone, 2% inoculum, 0.1 mM IPTG, 0.5 O.D., 100 mg/L CuSO<sub>4</sub>).</p>
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<p>Effect of (<b>A</b>): <b>medium composition</b> (37 °C, 24 h of fermentation, 7 pH, 2% inoculum, 0.1 mM IPTG, 0.4 O.D., 100 mg/L CuSO<sub>4</sub>); (<b>B</b>): <b>temperature</b> (YPD-Cu, 24 h of fermentation, 7 pH, 2% inoculum, 0.1 mM IPTG, 0.4 O.D., 100 mg/L CuSO<sub>4</sub>); (<b>C</b>): <b>fermentation time</b> (37 °C, YPD-Cu, 7 pH, 2% inoculum, 0.1 mM IPTG, 0.4 O.D., 100 mg/L CuSO<sub>4</sub>); (<b>D</b>): <b>pH</b> (37 °C, YPD-Cu, 24 h of fermentation, 2% inoculum, 0.1 mM IPTG, 0.4 O.D., 100 mg/L CuSO<sub>4</sub>); (<b>E</b>): <b>carbon source</b> (37 °C, 24 h of fermentation, 0.2% yeast extract, 0.5% peptone, 2% inoculum, 0.1 mM IPTG, 0.4 O.D., 100 mg/L CuSO<sub>4</sub>); (<b>F</b>): <b>concentration of carbon source</b> (37 °C, YPD-Cu, 24 h of fermentation, 0.2% yeast extract, 0.5% peptone, 2% inoculum, 0.1 mM IPTG, 0.5 O.D., 100 mg/L CuSO<sub>4</sub>).</p>
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<p>Effect of (<b>A</b>): <b>inorganic nitrogen source</b> (37 °C, 24 h of fermentation, 2% glucose, 2% inoculum, 0.1 mM IPTG, 0.4 O.D., 100 mg/L CuSO<sub>4</sub>); (<b>B</b>): <b>organic nitrogen source:</b> (37 °C, 24 h of fermentation, 2% glucose, 2% inoculum, 0.1 mM IPTG, 0.4 O.D., 100 mg/L CuSO<sub>4</sub>); (<b>C</b>): <b>concentration of nitrogen source</b> (37 °C, 24 h of fermentation, 2% glucose, 2% inoculum, 0.1 mM IPTG, 0.4 O.D., 100 mg/L CuSO<sub>4</sub>); (<b>D</b>): <b>inoculum size:</b> (37 °C, 24 h of fermentation, 2% glucose, 5% yeast extract, 0.1 mM IPTG, 0.4 O.D., 100 mg/L CuSO<sub>4</sub>).</p>
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<p>Effect of (<b>A</b>): <b>inorganic nitrogen source</b> (37 °C, 24 h of fermentation, 2% glucose, 2% inoculum, 0.1 mM IPTG, 0.4 O.D., 100 mg/L CuSO<sub>4</sub>); (<b>B</b>): <b>organic nitrogen source:</b> (37 °C, 24 h of fermentation, 2% glucose, 2% inoculum, 0.1 mM IPTG, 0.4 O.D., 100 mg/L CuSO<sub>4</sub>); (<b>C</b>): <b>concentration of nitrogen source</b> (37 °C, 24 h of fermentation, 2% glucose, 2% inoculum, 0.1 mM IPTG, 0.4 O.D., 100 mg/L CuSO<sub>4</sub>); (<b>D</b>): <b>inoculum size:</b> (37 °C, 24 h of fermentation, 2% glucose, 5% yeast extract, 0.1 mM IPTG, 0.4 O.D., 100 mg/L CuSO<sub>4</sub>).</p>
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<p>Effect of (<b>A</b>): <b>inducer type</b> (37 °C, 24 h of fermentation, 2% glucose, 5% yeast extract, 4% inoculum, 0.4 O.D., 100 mg/L CuSO<sub>4</sub>); (<b>B</b>): <b>inducer concentration</b> (37 °C, 24 h of fermentation, 2% glucose, 5% yeast extract, 4% inoculum, 0.4 O.D., 100 mg/L CuSO<sub>4</sub>); (<b>C</b>): <b>optical density</b> (37 °C, 24 h of fermentation, 2% glucose, 5% yeast extract, 4% inoculum, 0.1 mM IPTG, 100 mg/L CuSO<sub>4</sub>); (<b>D</b>): <b>CuSO<sub>4</sub> concentration</b> (37 °C, 24 h of fermentation, 2% glucose, 5% yeast extract, 4% inoculum, 0.1 mM IPTG, 0.4 O.D.).</p>
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<p>Effect of (<b>A</b>): <b>inducer type</b> (37 °C, 24 h of fermentation, 2% glucose, 5% yeast extract, 4% inoculum, 0.4 O.D., 100 mg/L CuSO<sub>4</sub>); (<b>B</b>): <b>inducer concentration</b> (37 °C, 24 h of fermentation, 2% glucose, 5% yeast extract, 4% inoculum, 0.4 O.D., 100 mg/L CuSO<sub>4</sub>); (<b>C</b>): <b>optical density</b> (37 °C, 24 h of fermentation, 2% glucose, 5% yeast extract, 4% inoculum, 0.1 mM IPTG, 100 mg/L CuSO<sub>4</sub>); (<b>D</b>): <b>CuSO<sub>4</sub> concentration</b> (37 °C, 24 h of fermentation, 2% glucose, 5% yeast extract, 4% inoculum, 0.1 mM IPTG, 0.4 O.D.).</p>
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<p>Precipitation of textile dyes carried out by recombinant laccase.</p>
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<p>The precipitation percentage of dyes carried out by rILac.</p>
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<p>FTIR analysis of the dyes before and after treatment with rILac.</p>
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<p>UV–Vis spectrophotometer analysis of the dyes before and after treatment with rILac.</p>
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<p>UV–Vis spectrophotometer analysis of the dyes before and after treatment with rILac.</p>
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24 pages, 1325 KiB  
Review
From Cradle to Grave: Microplastics—A Dangerous Legacy for Future Generations
by Tamara Lang, Filip Jelić and Christian Wechselberger
Environments 2024, 11(12), 263; https://doi.org/10.3390/environments11120263 - 22 Nov 2024
Abstract
Microplastics have become a ubiquitous pollutant that permeates every aspect of our environment—from the oceans to the soil to the elementary foundations of human life. New findings demonstrate that microplastic particles not only pose a latent threat to adult populations, but also play [...] Read more.
Microplastics have become a ubiquitous pollutant that permeates every aspect of our environment—from the oceans to the soil to the elementary foundations of human life. New findings demonstrate that microplastic particles not only pose a latent threat to adult populations, but also play a serious role even before birth during the fetal stages of human development. Exposure to microplastics during the early childhood stages is another source of risk that is almost impossible to prevent. This comprehensive review examines the multiple aspects associated with microplastics during early human development, detailing the mechanisms by which these particles enter the adult body, their bioaccumulation in tissues throughout life and the inevitable re-entry of these particles into different ecosystems after death. Full article
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<p>Accumulation of MPs in different tissues. (Created in BioRender. Lang, T. (2024) <a href="https://BioRender.com/w89g368" target="_blank">https://BioRender.com/w89g368</a>, accessed on 17 November 2024).</p>
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<p>Microplastics uptake during different stages of human development. (Created in BioRender. Lang, T. (2024) <a href="https://BioRender.com/d17c062" target="_blank">https://BioRender.com/d17c062</a>, accessed on 15 November 2024).</p>
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22 pages, 3073 KiB  
Article
Effects of Rhizobacteria Strains on Plant Growth Promotion in Tomatoes (Solanum lycopersicum)
by Eduardo Hernández-Amador, David Tomás Montesdeoca-Flores, Néstor Abreu-Acosta and Juan Cristo Luis-Jorge
Plants 2024, 13(23), 3280; https://doi.org/10.3390/plants13233280 - 22 Nov 2024
Abstract
Numerous factors, such as soil fertility, climatic conditions, human activity, pests, and diseases, limit agricultural yields. Pesticides and fertilizers have become indispensable tools to satisfy the global food demand. However, its adverse environmental effects have led to the search for more sustainable and [...] Read more.
Numerous factors, such as soil fertility, climatic conditions, human activity, pests, and diseases, limit agricultural yields. Pesticides and fertilizers have become indispensable tools to satisfy the global food demand. However, its adverse environmental effects have led to the search for more sustainable and ethical techniques. Biofertilizers and biopesticides based on plant- growth-promoting rhizobacteria (PGPRs) are efficient and ecological treatments that promote plant growth and protection against pathogens and abiotic stresses. In this study, twelve rhizobacterial strains with plant-growth-promoting attributes were selected to evaluate their plant-growth-promoting effect on tomato plants (Solanum lycopersicum L. var Robin). Soil inoculation with these strains resulted in a significant increase in shoot length, up to 50% when compared with control plants. Regarding fresh biomass, rhizobacterial treatments significantly improved seedlings’ fresh aerial weight with a maximum increase of 77%. Root biomass also demonstrated a substantial improvement, yielding 62.26% greater fresh root weight compared to the control. Finally, dry root weights exhibited the most remarkable enhancements, with values between 49 and 124%, when compared to the control plants. Concerning the nutritional status, the strains inoculation increased the macronutrients and micronutrients content in the aerial and root parts of the plants. All these findings suggest that rhizobacteria from different ecosystems and agriculture soils of the Canary Islands could be used as fertilizer inoculants to increase crop yield and promote more sustainable practices in modern agriculture. Full article
(This article belongs to the Section Crop Physiology and Crop Production)
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<p>Kruskal–Wallis tests for the six morphological variables studied. Note the clear difference between the three culture batches. (<b>A</b>) Shoot length (cm). (<b>B</b>) Root length (cm). (<b>C</b>) Fresh shoot mass (g). (<b>D</b>) Fresh root mass (g). (<b>E</b>) Dry shoot mass (g). (<b>F</b>) Dry shoot mass (g). Error bars represent standard deviation. The circle refers to outliers. The “*” refers to extreme outliers.</p>
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<p>Kruskal–Wallis tests for the six morphological variables studied. Note the clear difference between the three culture batches. (<b>A</b>) Shoot length (cm). (<b>B</b>) Root length (cm). (<b>C</b>) Fresh shoot mass (g). (<b>D</b>) Fresh root mass (g). (<b>E</b>) Dry shoot mass (g). (<b>F</b>) Dry shoot mass (g). Error bars represent standard deviation. The circle refers to outliers. The “*” refers to extreme outliers.</p>
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<p>Kruskal–Wallis tests for the six morphological variables studied. Note the clear difference between the three culture batches. (<b>A</b>) Shoot length (cm). (<b>B</b>) Root length (cm). (<b>C</b>) Fresh shoot mass (g). (<b>D</b>) Fresh root mass (g). (<b>E</b>) Dry shoot mass (g). (<b>F</b>) Dry shoot mass (g). Error bars represent standard deviation. The circle refers to outliers. The “*” refers to extreme outliers.</p>
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<p>Kruskal–Wallis tests for the two physiological parameters studied. (<b>A</b>) Stomatal conductance. (<b>B</b>) Photosynthesis rate. Error bars represent standard deviation. The circle refers to outliers. The “*” refers to extreme outliers.</p>
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<p>Kruskal–Wallis test for phenol and antioxidant content per gram of extract. (<b>A</b>) mg g<sup>−1</sup> of Galic acid—phenol. (<b>B</b>) mg g<sup>−1</sup> of Trolox—antioxidant. Error bars represent standard deviation.</p>
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23 pages, 5173 KiB  
Article
Multi-Criteria Filtration and Extraction Strategy for Understory Elevation Control Points Using ICESat-2 ATL08 Product
by Jiapeng Huang, Yunqiu Wang and Yang Yu
Forests 2024, 15(12), 2064; https://doi.org/10.3390/f15122064 - 22 Nov 2024
Abstract
Understory terrain plays a multi-faceted role in ecosystems, biodiversity, and productivity in forests by influencing different major factors, such as hydrological processes, soils, climate, and light conditions. Strong illuminants (e.g., sunlight) from ground surfaces and atmosphere can introduce additional photons into the ATLAS [...] Read more.
Understory terrain plays a multi-faceted role in ecosystems, biodiversity, and productivity in forests by influencing different major factors, such as hydrological processes, soils, climate, and light conditions. Strong illuminants (e.g., sunlight) from ground surfaces and atmosphere can introduce additional photons into the ATLAS system. These photons can, consequently, be mistakenly identified as laser photons reflected from ground surfaces. The presence of such ambient light, particularly under low-photon-count conditions, can significantly increase elevation measurement errors. In this context, this study aims to propose a method for extracting reliable understory elevation control points under varying forest conditions, based on the parameter attributes of ICESat-2/ATLAS data. The overall filtered data resulted in a coefficient of determination (R2), root mean square error (RMSE), and standard deviation (STD) of 0.99, 2.77 m, and 2.42 m, respectively. The greatest accuracy improvement was found in the Puerto Rico study area, showing decreases in the RMSE and STD values by 2.68 and 2.67 m, respectively. On the other hand, canopy heights and slopes exhibited relatively large impacts on noise interferences. In addition, there were decreases in the RMSE and STD values by 4.57 and 4.64 m, respectively, under the very tall canopy category, whereas under steep slope conditions, the RMSE and STD values of the filtering results decreased by 4.59 and 4.34 m, respectively. The proposed method can enhance the overall accuracy of elevation data, allowing for the significant extraction of understory elevation control points, ultimately optimizing forest management practices and improving ecological assessments. Full article
(This article belongs to the Special Issue LiDAR Remote Sensing for Forestry)
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<p>Forest types in the study areas: (<b>a</b>) California research area. (<b>b</b>) Quintana Roo area. (<b>c</b>) Puerto Rico area. (<b>d</b>) South Carolina area.</p>
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<p>Flowchart of the methodology adopted in this study.</p>
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<p>Error histogram of the study area: (<b>a</b>) California research area. (<b>b</b>) Quintana Roo area. (<b>c</b>) Puerto Rico area. (<b>d</b>) South Carolina area.</p>
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<p>Histogram of error distribution for landform types: (<b>a</b>) Lowlands. (<b>b</b>) Highlands. (<b>c</b>) Plateau. (<b>d</b>) Mountains. (<b>e</b>) Hills.</p>
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<p>Histogram of error distribution for slope types: (<b>a</b>) Steep slopes. (<b>b</b>) Gentle slopes. (<b>c</b>) Abrupt slopes. (<b>d</b>) Flat slopes. (<b>e</b>) Inclined slopes.</p>
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<p>Histogram of error distribution for aspect: (<b>a</b>) North; (<b>b</b>) east; (<b>c</b>) northeast; (<b>d</b>) southeast; (<b>e</b>) south; (<b>f</b>) northwest; (<b>g</b>) west; (<b>h</b>) southwest.</p>
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<p>Histogram of error distribution for surface cover types: (<b>a</b>) closed evergreen broadleaf forest; (<b>b</b>) closed evergreen coniferous forest; (<b>c</b>) closed mixed-leaf forest; (<b>d</b>) closed deciduous broadleaf forest; (<b>e</b>) closed deciduous coniferous forest; (<b>f</b>) shrubland; (<b>g</b>) open evergreen coniferous forest; (<b>h</b>) tropical rainforest.</p>
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<p>Error histogram of tree height: (<b>a</b>) large trees; (<b>b</b>) shrubs; (<b>c</b>) error histogram of tall trees; (<b>d</b>) small trees; (<b>e</b>) medium trees.</p>
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13 pages, 1648 KiB  
Article
Biomimetic Plant-Root-Inspired Robotic Sensor System
by Margarita Alvira, Alessio Mondini, Gian Luigi Puleo, Islam Bogachan Tahirbegi, Lucia Beccai, Ali Sadeghi, Barbara Mazzolai, Mònica Mir and Josep Samitier
Biosensors 2024, 14(12), 565; https://doi.org/10.3390/bios14120565 - 22 Nov 2024
Abstract
There are many examples in nature in which the ability to detect is combined with decision-making, such as the basic survival instinct of plants and animals to search for food. We can technically translate this innate function via the use of robotics with [...] Read more.
There are many examples in nature in which the ability to detect is combined with decision-making, such as the basic survival instinct of plants and animals to search for food. We can technically translate this innate function via the use of robotics with integrated sensors and artificial intelligence. However, the integration of sensing capabilities into robotics has traditionally been neglected due to the significant associated technical challenges. Inspired by plant-root chemotropism, we present a miniaturized electrochemical array integrated into a robotic tip, embedding a customized micro-potentiometer. The system contains solid-state sensors fitted to the tip of the robotic root to three-dimensionally monitor potassium and pH changes in a moist, soil-like environment, providing an integrated electronic readout. The sensors measure a range of parameters compatible with realistic soil conditions. The sensors’ response can trigger the movement of the robotic root with a control algorithm inspired by the behavior of the plant root that determines the optimal path toward root growth, simulating the decision-making process of a plant. This nature-inspired technology may lead, in the future, to the realization of robotic devices with the potential for monitoring and exploring the soil autonomously. Full article
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<p>Potentiometric response (n = 5) of (<b>A</b>) pH sensor in 0.1 M Tris-HCl solutions of different pH values and (<b>B</b>) K<sup>+</sup> sensor in 0.1 M Tris-HCl solution (pH 7.4) of different KCl concentrations.</p>
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<p>Potentiometric response (n = 5) of (<b>A</b>) a pH sensor in gels made with Phytagel<sup>TM</sup> powder and 0.1 M Tris·HCl solutions of different pH values and (<b>B</b>) a K<sup>+</sup> sensor in gels made with Phytagel<sup>TM</sup> powder and 0.1 M Tris·HCl solution (pH 7.4) of different KCl concentrations.</p>
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<p>Scheme of the robotic biomimetic root with sensors integrated. (<b>A</b>) Soft-bending mechanism design. (<b>B</b>) Prototype. (<b>C</b>) Root tip prototype with integrated chemical sensors. (<b>D</b>) ISE prototype. (<b>E</b>) Micro-potentiometer integrated in the root tip. (<b>F</b>) Sensors’ position with respect to the x–y axis of the embedded accelerometer. (<b>G</b>) Tip architecture with chemical sensor’s front-end schematization for each of the three sensor arrays.</p>
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<p>Comparison between pH (<b>A</b>) and K<sup>+</sup> (<b>B</b>) measurements performed on Phytagel<sup>TM</sup> G1–9 with commercial potentiostat (squared black data) and with the Plantoid robot (circled red data) n = 4.</p>
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<p>Root-based robotic sensor’s response to different pH and potassium stimuli. (<b>A</b>) Gel application on the ISE sensors. (<b>B</b>) Bending of the robotic root used for tropism experiments. (<b>C</b>) K<sup>+</sup> sensors’ response from the arrays on the robot tip under different stimuli. (<b>D</b>) Data acquired from the pH sensors from the arrays on the robot tip under different stimuli. (<b>E</b>) Tip angle extracted by the accelerometer data. These angles are shown in the tip by vectors: <span class="html-italic">θ</span> is the rotation of the tip with respect to the <span class="html-italic">x</span>-axes, and <span class="html-italic">φ</span> is the bending represented as the module of the vector.</p>
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