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Search Results (32,652)

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12 pages, 938 KiB  
Article
Low-Cost Optical Filters Based on SiOxCy:H and Ag Thin Films Fabricated by Plasma Enhanced Chemical Vapor Deposition and Sputtering
by Ahmed Kotbi, Michael Lejeune, Pierre Barroy, Ilham Hamdi Alaoui, Wiaam El Hakim, Frederic Lamarque and Andreas Zeinert
Photonics 2024, 11(11), 1096; https://doi.org/10.3390/photonics11111096 (registering DOI) - 20 Nov 2024
Abstract
Hexamethyldisiloxane (HMDSO) is an organosilicon compound with a modifiable bandgap, depending on the deposition conditions. This material has many unique properties due to its stability, low toxicity, and strong adhesion, making it useful as a protective barrier against corrosion, moisture, and oxidation. In [...] Read more.
Hexamethyldisiloxane (HMDSO) is an organosilicon compound with a modifiable bandgap, depending on the deposition conditions. This material has many unique properties due to its stability, low toxicity, and strong adhesion, making it useful as a protective barrier against corrosion, moisture, and oxidation. In this work, HMDSO films were deposited on glass substrates by the Plasma Enhanced Chemical Vapor Deposition (PECVD) technique at different deposition times. The optical properties of HMDSO films, such as dielectric permittivity, refractive index, extinction and absorption coefficients, and band gap energy, are inferred from transmission and reflection spectra. As the deposition time increased, the real part of the dielectric constant, the refractive index, and the bandgap energy showed a decrease, dropping from 4.24 to 3.40, from 2.06 to 1.84, and from 2.85 eV to 2.03 eV, respectively. The latter result is determined using classical models such as the O’Leary-Johnson-Lim (‘OJL’) interband transition and the harmonic oscillator model. HMDSO and Silver are used in this study for the fabrication of optical filters using two types of structures, a multiple cavity metal–dielectric (MCMD) and the Fabry–Perot structure. The silver layers are deposited by a sputtering process. The MCMD optical filter shows a higher transmittance of about 30%, but a wide range of wavelengths is transmitted. In contrast, the Fabry–Perot filter showed high contrast but a lower transmittance of about 20%. Full article
(This article belongs to the Section Optoelectronics and Optical Materials)
18 pages, 1687 KiB  
Article
Decision Analysis of Manufacturers’ Misreporting Behavior Considering Retailer Corporate Social Responsibility
by Jun Zhao, Tao Li, Qi Tan and Weirong Liu
Sustainability 2024, 16(22), 10151; https://doi.org/10.3390/su162210151 (registering DOI) - 20 Nov 2024
Abstract
Under the increasingly competitive environment, some manufacturers may misreport costs to boost their own profits, potentially harming the overall interests of the supply chain. Conversely, some retailers may engage in corporate social responsibility (CSR) to promote sustainable development within the supply chain. Thus, [...] Read more.
Under the increasingly competitive environment, some manufacturers may misreport costs to boost their own profits, potentially harming the overall interests of the supply chain. Conversely, some retailers may engage in corporate social responsibility (CSR) to promote sustainable development within the supply chain. Thus, this study considers a competitive supply chain comprising one retailer and two manufacturers, and investigates the impact of the manufacturer’s misreporting behavior on the chain supply members’ pricing decisions and performances as well as the impact of retailer CSR on the manufacturer’s misreporting behavior. When the retailer does not engage in CSR, the manufacturers’ misreporting behavior is solely influenced by misreporting factors. If the misreporting factors of both manufacturers are low, they can always benefit from their misreporting behavior. Interestingly, if one manufacturer has a high misreporting factor while the other has a low one, the manufacturer whose misreport factor is high does not misreport its cost, instead benefiting from the competitor’s misreporting behavior. When the retailer engages in CSR, the manufacturer’s misreporting behavior is influenced not only by their misreporting factors but also by the degree of product substitutability. If product substitutability is low, both manufacturers do not have an incentive to misreport their costs. If product substitutability is moderate and both misreporting factors are low, they can benefit from their misreporting behavior. However, when product substitutability is high, if one manufacturer misreports, the other will refrain from doing so. Full article
(This article belongs to the Section Sustainable Management)
27 pages, 1570 KiB  
Article
ImageOP: The Image Dataset with Religious Buildings in the World Heritage Town of Ouro Preto for Deep Learning Classification
by André Luiz Carvalho Ottoni and Lara Toledo Cordeiro Ottoni
Heritage 2024, 7(11), 6499-6525; https://doi.org/10.3390/heritage7110302 (registering DOI) - 20 Nov 2024
Abstract
Artificial intelligence has significant applications in computer vision studies for cultural heritage. In this research field, visual inspection of historical buildings and the digitization of heritage using machine learning models stand out. However, the literature still lacks datasets for the classification and identification [...] Read more.
Artificial intelligence has significant applications in computer vision studies for cultural heritage. In this research field, visual inspection of historical buildings and the digitization of heritage using machine learning models stand out. However, the literature still lacks datasets for the classification and identification of Brazilian religious buildings using deep learning, particularly with images from the historic town of Ouro Preto. It is noteworthy that Ouro Preto was the first Brazilian World Heritage Site recognized by UNESCO in 1980. In this context, this paper aims to address this gap by proposing a new image dataset, termed ImageOP: The Image Dataset with Religious Buildings in the World Heritage Town of Ouro Preto for Deep Learning Classification. This new dataset comprises 1613 images of facades from 32 religious monuments in the historic town of Ouro Preto, categorized into five classes: fronton (pediment), door, window, tower, and church. The experiments to validate the ImageOP dataset were conducted in two stages: simulations and computer vision using smartphones. Furthermore, two deep learning structures (MobileNet V2 and EfficientNet B0) were evaluated using Edge Impulse software. MobileNet V2 and EfficientNet B0 are architectures of convolutional neural networks designed for computer vision applications aiming at low computational cost, real-time classification on mobile devices. The results indicated that the models utilizing EfficientNet achieved the best outcomes in the simulations, with accuracy = 94.5%, precision = 96.0%, recall = 96.0%, and F-score = 96.0%. Additionally, superior accuracy values were obtained in detecting the five classes: fronton (96.4%), church (97.1%), window (89.2%), door (94.7%), and tower (95.4%). The results from the experiments with computer vision and smartphones reinforced the effectiveness of the proposed dataset, showing an average accuracy of 88.0% in detecting building elements across nine religious monuments tested for real-time mobile device application. The dataset is available in the Mendeley Data repository: Full article
9 pages, 2658 KiB  
Article
Gravure-Printed Anodes Based on Hard Carbon for Sodium-Ion Batteries
by Maria Montanino, Claudia Paoletti, Anna De Girolamo Del Mauro and Giuliano Sico
Batteries 2024, 10(11), 407; https://doi.org/10.3390/batteries10110407 - 20 Nov 2024
Abstract
Printed batteries are increasingly being investigated for feeding small, wearable devices more and more involved in our daily lives, promoting the study of printing technologies. Among these, gravure is very attractive as a low-cost and low-waste production method for functional layers in different [...] Read more.
Printed batteries are increasingly being investigated for feeding small, wearable devices more and more involved in our daily lives, promoting the study of printing technologies. Among these, gravure is very attractive as a low-cost and low-waste production method for functional layers in different fields, such as energy, sensors, and biomedical, because it is easy to scale up industrially. Thanks to our research, the feasibility of gravure printing was recently proved for rechargeable lithium-ion batteries (LiBs) manufacturing. Such studies allowed the production of high-quality electrodes involving different active materials with high stability, reproducibility, and good performance. Going beyond lithium-based storage devices, our attention was devoted on the possibility of employing highly sustainable gravure printing for sodium-ion batteries (NaBs) manufacturing, following the trendy interest in sodium, which is more abundant, economical, and ecofriendly than lithium. Here a study on gravure printed anodes for sodium-ion batteries based on hard carbon as an active material is presented and discussed. Thanks to our methodology centered on the capillary number, a high printing quality anodic layer was produced providing typical electrochemical behavior and good performance. Such results are very innovative and relevant in the field of sodium-ion batteries and further demonstrate the high potential of gravure in printed battery manufacturing. Full article
39 pages, 11304 KiB  
Review
An Overview of Sustainable Desalination with Freezing Crystallization: Current Development, Future Challenges, and Prospects
by Senyao Zhao, Rongjie Zhu, Jiatong Song and Han Yuan
Sustainability 2024, 16(22), 10138; https://doi.org/10.3390/su162210138 - 20 Nov 2024
Abstract
As global demand for freshwater grows, seawater desalination has become one of the most promising methods for obtaining freshwater. Many coastal nations have included it in their sustainable development plans and are actively advancing related technologies. Compared with traditional desalination methods, such as [...] Read more.
As global demand for freshwater grows, seawater desalination has become one of the most promising methods for obtaining freshwater. Many coastal nations have included it in their sustainable development plans and are actively advancing related technologies. Compared with traditional desalination methods, such as distillation and membrane-based desalination, seawater freezing desalination offers the benefit of producing large amounts of freshwater at lower costs. This study provides an overview of the main methods and principles of seawater freezing desalination and summarizes the latest research progress. This paper also discusses experimental and simulation studies of different principles. Current research shows that both direct and indirect seawater freezing desalination technologies have become relatively mature, laying a foundation for practical applications. Hydrate-based desalination, eutectic freezing technology, and vacuum freezing technology offer cost-reduction benefits, but existing technologies have limitations, making these areas hot topics in research. Additionally, this paper discusses the experimental progress and simulation methods associated with this, elaborates upon, and analyzes the freezing crystallization process and desalination efficiency from the perspective of the bottom layer of crystal growth, offering valuable insights for future research. It concludes by summarizing and predicting the development of these technologies, emphasizing their great potential due to their low-cost and sustainable features. Full article
(This article belongs to the Section Sustainable Engineering and Science)
19 pages, 3800 KiB  
Article
Fully Open-Source Meeting Minutes Generation Tool
by Amma Liesvarastranta Haz, Yohanes Yohanie Fridelin Panduman, Nobuo Funabiki, Evianita Dewi Fajrianti and Sritrusta Sukaridhoto
Future Internet 2024, 16(11), 429; https://doi.org/10.3390/fi16110429 - 20 Nov 2024
Abstract
With the increasing use of online meetings, there is a growing need for efficient tools that can automatically generate meeting minutes from recorded sessions. Current solutions often rely on proprietary systems, limiting adaptability and flexibility. This paper investigates whether various open-source models and [...] Read more.
With the increasing use of online meetings, there is a growing need for efficient tools that can automatically generate meeting minutes from recorded sessions. Current solutions often rely on proprietary systems, limiting adaptability and flexibility. This paper investigates whether various open-source models and methods such as audio-to-text conversion, summarization, keyword extraction, and optical character recognition (OCR) can be integrated to create a meeting minutes generation tool for recorded video presentations. For this purpose, a series of evaluations are conducted to identify suitable models. Then, the models are integrated into a system that is modular yet accurate. The utilization of an open-source approach ensures that the tool remains accessible and adaptable to the latest innovations, thereby ensuring continuous improvement over time. Furthermore, this approach also benefits organizations and individuals by providing a cost-effective and flexible alternative. This work contributes to creating a modular and easily extensible open-source framework that integrates several advanced technologies and future new models into a cohesive system. The system was evaluated on ten videos created under controlled conditions, which may not fully represent typical online presentation recordings. It showed strong performance in audio-to-text conversion with a low word-error rate. Summarization and keyword extraction were functional but showed room for improvement in terms of precision and relevance, as gathered from the users’ feedback. These results confirm the system’s effectiveness and efficiency in generating usable meeting minutes from recorded presentation videos, with room for improvement in future works. Full article
(This article belongs to the Special Issue Deep Learning and Natural Language Processing II)
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<p>Overview of meeting minutes generation system.</p>
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<p>Acceptable document format.</p>
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<p>Workflow for <span class="html-italic">information correlation function</span>.</p>
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<p>Sample of organized data for each slide.</p>
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<p>(<b>a</b>) Input field for recorded video and presentation document; (<b>b</b>) extracted results for each slide.</p>
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16 pages, 1521 KiB  
Article
Policy Recommendations to Enhance Small-to-Medium-Sized Enterprise Support for Achieving the UK’s Net Zero Targets
by Fatemeh Khosravi, Sam Jelliman, Chidinma Uchendu, Hebba Haddad, Alan Chandler and Stuart Connop
Sustainability 2024, 16(22), 10116; https://doi.org/10.3390/su162210116 - 20 Nov 2024
Viewed by 85
Abstract
In the UK, SMEs account for 99.9% of the business population, with from 43% to 53% of UK business emissions coming from SMEs. However, SMEs face specific barriers in relation to net zero targets, such as a lack of knowledge about strategies to [...] Read more.
In the UK, SMEs account for 99.9% of the business population, with from 43% to 53% of UK business emissions coming from SMEs. However, SMEs face specific barriers in relation to net zero targets, such as a lack of knowledge about strategies to achieve net zero targets. Despite the ongoing lack of a formal decarbonisation plan for SMEs, various types of projects have been designed to help SMEs to move towards the UK’s net zero target. The Eastern New Energy (ENE) project received funding from the European Regional Development Fund (ERDF) to support SMEs across significant economic areas of the UK, namely the East of England, to help address the barriers that prevent SMEs from decarbonising. The project provided a range of measures, including raising their level of knowledge and providing technical support to help with SME decarbonisation. Following the completion of our targeted support activities, semi-structured interviews (n = 21) were conducted with SME beneficiaries to evaluate the effectiveness of the support with the ambition of providing some policy recommendations based on understanding how national and strategic support can be effective for a diverse, often unconnected ecosystem of businesses. The findings from the interviews indicated that engagement in the ENE project had positive impacts on the SMEs’ understanding of net zero, or how to start their journey towards decarbonisation by identifying their source of emissions and learning how to calculate them. However, accounting for emissions from Scope 3 activities, transition costs and building restrictions were the main barriers that undermined the ENE recommendations. Our research concludes that in designing engagement projects and support, one needs to acknowledge such restrictions and barriers, which requires government interventions to cover all aspects of the SME decarbonisation journey, from nuanced and responsive policy making to developing bespoke knowledge transfer and supporting low-carbon material provision that can support a decarbonised marketplace. Full article
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<p>KEP workshop plan.</p>
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<p>Types and number of support interventions provided by the ENE team (n = 39).</p>
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<p>Interviewees categorised by the type of ENE support received.</p>
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<p>Effect of ENE involvement on SMEs’ understanding of net zero meaning.</p>
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<p>Effect of ENE support on SMEs’ net zero journey.</p>
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<p>SMEs’ net zero journey capacity building pyramid (adapted from Potter and Brough, 2004 [<a href="#B40-sustainability-16-10116" class="html-bibr">40</a>]).</p>
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14 pages, 233 KiB  
Article
Assessing Knowledge, Practices, and Barriers to PrEP and nPEP Prescription Among Texas Healthcare Providers
by Yordanos M. Tiruneh, Ruchi Rachmale, Nagla Elerian and David L. Lakey
Healthcare 2024, 12(22), 2315; https://doi.org/10.3390/healthcare12222315 - 20 Nov 2024
Viewed by 74
Abstract
Background: The effectiveness of pre-exposure prophylaxis (PrEP) and non-occupational post-exposure prophylaxis (nPEP) in preventing HIV is well-established, yet their use in clinical practice remains low. Healthcare providers, especially those in primary and emergency care settings, play a crucial role in adopting and implementing [...] Read more.
Background: The effectiveness of pre-exposure prophylaxis (PrEP) and non-occupational post-exposure prophylaxis (nPEP) in preventing HIV is well-established, yet their use in clinical practice remains low. Healthcare providers, especially those in primary and emergency care settings, play a crucial role in adopting and implementing these prevention strategies. We conducted a statewide survey with 519 healthcare providers in Texas to assess their knowledge, practices, and barriers related to prescribing PrEP and nPEP. Methods: The survey collected data on demographics, clinical experience, practice type, awareness of recommended guidelines, knowledge of PrEP and nPEP, prescribing practices, and challenges encountered to prescribe these medications. We used multiple regression analysis to identify factors associated with PrEP and nPEP prescribing behavior. Results: While most providers were familiar with CDC and/or USPSTF-recommended PrEP guidelines, fewer had adequate knowledge of nPEP. Key challenges identified by providers included concerns about cost (48%), limited time (40% for PrEP and 51% for nPEP), and insufficient education or training (40% for PrEP and 35% for nPEP). Providers who were more familiar with the recommended guidelines and had greater experience in sexual health assessment were more likely to prescribe both PrEP and nPEP. Conclusions: This study highlights the need for enhanced education and training to boost providers’ knowledge and confidence in prescribing PrEP and nPEP. It also underscores the importance of addressing cost-related barriers and simplifying care processes to better integrate these HIV prevention strategies into primary and emergency care settings. Full article
9 pages, 2156 KiB  
Article
Phase Retrieval of One-Dimensional Objects by the Multiple-Plane Gerchberg–Saxton Algorithm Implemented into a Digital Signal Processor
by Varis Karitans, Maris Ozolinsh and Sergejs Fomins
Optics 2024, 5(4), 514-522; https://doi.org/10.3390/opt5040038 - 20 Nov 2024
Viewed by 67
Abstract
In the current study, we address the phase retrieval of one-dimensional phase objects from near-field diffraction patterns using the multiple-plane Gerchberg–Saxton algorithm, which is still widely used for phase retrieval. The algorithm was implemented in a low-cost digital signal processor capable of fast [...] Read more.
In the current study, we address the phase retrieval of one-dimensional phase objects from near-field diffraction patterns using the multiple-plane Gerchberg–Saxton algorithm, which is still widely used for phase retrieval. The algorithm was implemented in a low-cost digital signal processor capable of fast Fourier transform using Q15 arithmetic, which is used by the previously mentioned algorithm. We demonstrate similarity between one-dimensional phase objects, i.e., vectors cut out of a phase map of the tertiary spherical aberration retrieved by the multiple-plane Gerchberg–Saxton algorithm, and these vectors are measured with a non-contact profiler. The tertiary spherical aberration was induced by a phase plate fabricated using grayscale lithography. After subtracting the vectors retrieved by the algorithm from those measured with the profiler, the root mean square error decreased, while a corresponding increase in the Strehl ratio was observed. A single vector of size 64 pixels was retrieved in about 2 min. The results suggest that digital signal processors that are capable of one-dimensional FFT and fixed-point arithmetic in Q15 format can successfully retrieve the phase of one-dimensional objects, and they can be used for applications that do not require real-time operation, i.e., analyzing the quality of cylindrical micro-optics. Full article
(This article belongs to the Section Engineering Optics)
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<p>The basic circuit for programming a digital signal processor using MPLAB ICD 4. The resistance of the resistor is 50 kΩ. The actual digital signal processor used in the study was dsPIC30F6010A. Five lines are required for communication between MPLAB ICD 4 and the digital signal processor: V<sub>dd</sub>, V<sub>ss</sub>, V<sub>pp</sub>/MCLR, PGC, and PGD.</p>
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<p>The experimental setup used in the study. L stands for lens; M stands for mirror. The two-dimensional object is shown in the bottom right, and the slit was used to cut out one-dimensional objects from it. These objects were then retrieved using the MPGS algorithm. The phase distortions are given in radians. Abbreviations: C—a fiber collimator, M—mirrors, L—lenses, O—the two-dimensional object.</p>
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<p>Diffraction patterns of the test objects, i.e., the 21st, the 33rd, and the 55th vectors (counting from the bottom). The vectors, i.e., the test objects, were cut out from the two-dimensional object via the slit. The planes change along the horizontal direction (from 1 to 5).</p>
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<p>The left column shows the phase (in radians) of the test objects, i.e., the 55th (<b>top</b>), the 33rd (<b>middle</b>), and the 21st (<b>bottom</b>) vectors measured with the ground-truth method vs. the horizontal coordinate of the test object (in pixels). One pixel of the <span class="html-italic">x</span>-axis is equal to 7.5 μm. The right column shows the corresponding objects retrieved with the MPGS algorithm.</p>
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<p>The residual phase (in radians) of the tests objects, i.e., the 55th (<b>top</b>), the 33rd (<b>middle</b>), and the 21st (<b>bottom</b>) vectors vs. the horizontal coordinate of the test object (in pixels). One pixel of the <span class="html-italic">x</span>-axis is equal to 7.5 μm. The residual phase was calculated by subtracting the retrieved phase from that measured with the non-contact profiler.</p>
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13 pages, 5261 KiB  
Article
A High-Performance Micro Differential Pressure Sensor
by Xutao Fan, Lei Wang and Songsong Zhang
Micromachines 2024, 15(11), 1396; https://doi.org/10.3390/mi15111396 - 20 Nov 2024
Viewed by 41
Abstract
With the development of the micro electromechanical system (MEMS), which widely adopts micro differential pressure sensors (MDPSs), the demand for high-performance MDPSs had continuously increased. Pressure sensors realized using MEMS technology integrated with biomedical catheters are of significant importance in the detection and [...] Read more.
With the development of the micro electromechanical system (MEMS), which widely adopts micro differential pressure sensors (MDPSs), the demand for high-performance MDPSs had continuously increased. Pressure sensors realized using MEMS technology integrated with biomedical catheters are of significant importance in the detection and treatment of various biological diseases. Biomedical catheters used in low-Fr applications (1Fr = 0.33 mm outer diameter) require miniaturized sensors that do not compromise their performance. For instance, catheters (5Fr) used for central venous pressure (CVP) monitoring require the integration of high-performance sensors with total dimensions smaller than 1.65 mm along at least two directions (length, width, or height). In this paper, a silicon-on-insulator (SOI)-based MDPS was designed and fabricated for micro-pressure detection in the range of 0–1 kPa. The dimension of the sensor is only 1 mm × 1 mm × 0.4 mm, with a sensitivity of 3.401 mV/V/kPa at room temperature, nonlinearity of 0.376% FS (full scale), and an overall accuracy of 0.59% FS. The sensor operates normally when the temperature is even increased to 160 °C, and its temperature coefficient of zero output (TCO) and temperature coefficient of sensitivity (TCS) are 0.093% FS/°C and −0.144% FS/°C. The dimension and performance results of this MDPS demonstrate its potential to play a significant role in biomedical catheters. In addition, it is fabricated using an 8-inch MEMS process, which significantly reduces the cost. Full article
(This article belongs to the Special Issue Acoustic Transducers and Their Applications, 2nd Edition)
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<p>(<b>a</b>) Schematic diagram of a typical P-type piezoresistive resistor. (<b>b</b>) Wheatstone bridge diagram.</p>
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<p>P (N, T) coefficient of P-type silicon vs. doping concentration for different temperatures.</p>
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<p>(<b>a</b>) Schematic diagram of inductive diaphragm. (<b>b</b>) Stress distribution on the surface of a piezoresistor at 10 μm from the edge of the diaphragm under an applied pressure of 1 kPa.</p>
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<p>Distribution of doping concentration of the piezoresistor in the depth direction for different device layer thicknesses.</p>
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<p>Simulation results for different diaphragm thicknesses W: (<b>a</b>) Output vs. Pressure. (<b>b</b>) Sensitivity vs. Temperature.</p>
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<p>Three-dimensional model drawing of the sensor (values is μm).</p>
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<p>Manufacturing process flowchart and 8-inch MEMS wafer of the pressure sensor. (<b>a</b>) thermal oxidation; (<b>b</b>) boron light doping; (<b>c</b>) boron heave doping; (<b>d</b>) excess silicon; (<b>e</b>) deposition of silica and metal wiring; (<b>f</b>) back cavity release.</p>
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<p>Profiler result plot of the pressure sensor.</p>
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<p>Diagram of the pressure sensor test environment. (<b>a</b>) gas pressure source; (<b>b</b>) pressure controller; (<b>c</b>) DC power analyzer; (<b>d</b>) test sample; (<b>e</b>) semiconductor device analyzer; (<b>f</b>) digital multimeter; (<b>g</b>) temperature-controlled heating stage.</p>
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<p>(<b>a</b>) Static performance test chart of the sensor. (<b>b</b>) Enlargement of detail from <a href="#micromachines-15-01396-f010" class="html-fig">Figure 10</a>a.</p>
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<p>Temperature performance of the sensor.</p>
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15 pages, 4604 KiB  
Article
Development of an Electrochemical Paper-Based Device Modified with Functionalized Biochar for the Screening of Paracetamol in Substandard Medicines
by Martin Kassio Leme da Silva, Francisco Contini Barreto, Guilherme dos Santos Sousa, Rafael Plana Simões, Gaurav Ahuja, Samriddha Dutta, Ashok Mulchandani and Ivana Cesarino
Molecules 2024, 29(22), 5468; https://doi.org/10.3390/molecules29225468 - 20 Nov 2024
Viewed by 111
Abstract
The global prevalence of counterfeit and low-quality pharmaceuticals poses significant health risks and challenges in medical treatments, creating a need for rapid and reliable drug screening technologies. This study introduces a cost-effective electrochemical paper-based device (ePAD) modified with functionalized bamboo-derived biochar (BCF) for [...] Read more.
The global prevalence of counterfeit and low-quality pharmaceuticals poses significant health risks and challenges in medical treatments, creating a need for rapid and reliable drug screening technologies. This study introduces a cost-effective electrochemical paper-based device (ePAD) modified with functionalized bamboo-derived biochar (BCF) for the detection of paracetamol in substandard medicines. The sensor was fabricated using a custom 3D-printed stencil in PLA, designed for efficient production, and a 60:40 (m/m) graphite (GR) and glass varnish (GV) conductive ink, resulting in a robust and sensitive platform. The electroactive area of the ePAD/BCF sensor was determined as 0.37 cm2. Characterization via SEM and cyclic voltammetry (CV) verified its structural and electrochemical stability. The sensor demonstrated linear detection of paracetamol from 5.0 to 60.0 µmol L−1 with a detection limit of 3.50 µmol L−1. Interference studies showed high selectivity, with recoveries of over 90%, and the sensor successfully quantified paracetamol in commercial analgesic and anti-flu samples. This sustainable, bamboo-based ePAD offers a promising solution for rapid on-site pharmaceutical quality control, with significant potential to enhance drug screening accuracy. Full article
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<p>SEM images of carbon ink spread on a paper substrate at magnifications of (<b>A</b>) 5000× and (<b>B</b>) 10,000×; (<b>C</b>,<b>D</b>) ePAD modified with BCF.</p>
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<p>Cyclic voltammograms in PBS 0.2 mol L<sup>−1</sup> containing 0.1 mol L<sup>−1</sup> of KCl and 5.0 mmol L<sup>−1</sup> of [Fe(CN)<sub>6</sub>]<sup>3−/4−</sup>: (<b>A</b>) Comparison of ePAD performances with different formulations of GR-GV conductive ink. (<b>B</b>) Cyclic voltammograms of ePADs fabricated with different conductive ink compositions (Ink 1: 80:20, Ink 2: 70:30, Ink 3: 60:40, and Ink 4: 50:50 GR ratios, m/m) in 0.1 mol L<sup>−1</sup> PBS containing 5.0 mmol L<sup>−1</sup> [Fe(CN)<sub>6</sub>]<sup>3−</sup>/<sup>4−</sup> at 50 mV s<sup>−1</sup>. (<b>C</b>) Voltammetric response of the ePAD sensor without modification (red line) and after BCF modification (black line).</p>
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<p>(<b>A</b>) Cyclic voltammograms obtained with the ePAD/BCF sensor in a 0.2 mol L<sup>−1</sup> PBS solution (pH 7.4) containing 0.1 mol L<sup>−1</sup> of KCl and 5.0 mmol L<sup>−1</sup> of the redox couple [Fe(CN)<sub>6</sub>]<sup>3−/4−</sup> for scanning speeds of 10, 25, 50, 75 and 100 mV s<sup>−1</sup>. (<b>B</b>) Graph of <span class="html-italic">I</span> vs. <span class="html-italic">v</span><sup>1/2</sup>.</p>
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<p>(<b>A</b>) Cyclic voltammograms of the ePAD/BCF sensor in the absence (dashed line) and presence of 200 μmol L<sup>−1</sup> of PARA in 0.1 mol L<sup>−1</sup> of PBS (pH 7.0), <span class="html-italic">v</span> = 50 mV s<sup>−1</sup>. (<b>B</b>) Paracetamol oxidation mechanism to N-acetyl-p-benzoquinonaimine on the ePAD/BCF sensor.</p>
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<p>LSV voltammograms obtained with the ePAD sensor in the absence (dashed line) and in the presence (curve b) of 50 μmol L<sup>−1</sup> of PARA, as well as the ePAD/BCF sensor in the presence of 50 μmol L<sup>−1</sup> of PARA (curve c) (<span class="html-italic">v</span> = 25 mV s<sup>−1</sup>).</p>
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<p>(<b>A</b>) LSV voltammograms obtained with 150 μL of 0.1 mol L<sup>−1</sup> PBS solution, pH 7.0 with different concentrations of PARA ranging from 5.0 to 60 μmol L<sup>−1</sup> and (<b>B</b>) linear relationship between anodic peak current and PARA concentration.</p>
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<p>LSV voltammograms with 20 µmol L<sup>−1</sup> of PARA, showing the electrochemical response before and after the addition of (<b>A</b>) starch, (<b>B</b>) PEG, (<b>C</b>) PVA and (<b>D</b>) sodium chloride (1:1 ratio). These additions assess the impact of common matrix components on PARA detection.</p>
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<p>Linear sweep voltammetry (LSV) responses for a flu medicine sample with an initial concentration of 50 µM paracetamol, recorded using a graphite-based ePAD sensor. The red curve represents the LSV response of the original sample (50 µM), while subsequent standard additions of paracetamol at concentrations of 10 µM (green), 20 µM (blue) and 30 µM (cyan) show progressive increases in peak current. The inset displays a linear calibration plot of peak current (<span class="html-italic">I</span><sub>pa</sub>) vs. C<sub>PARA</sub>, validating the linearity and accuracy of the standard addition method for quantifying paracetamol in complex matrices.</p>
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<p>Sequential fabrication of ePADs: (<b>A</b>) Stencil design conceptualized in PLA for sensor creation. (<b>B</b>) Stencil produced using PLA filament via 3D printing. (<b>C</b>) Preparation of conductive ink. (<b>D</b>) Definition of the electrolyte area, including the following steps: (I) digital design, (II) wax printing, (III) wax melting to create a hydrophobic barrier within the paper, (IV) sensor fabrication using the stencil and conductive ink and (V) final assembly of the ePAD. (<b>E</b>) Setup adjustment for electrochemical measurements with metallic clips.</p>
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18 pages, 12381 KiB  
Article
AQSA—Algorithm for Automatic Quantification of Spheres Derived from Cancer Cells in Microfluidic Devices
by Ana Belén Peñaherrera-Pazmiño, Ramiro Fernando Isa-Jara, Elsa Hincapié-Arias, Silvia Gómez, Denise Belgorosky, Eduardo Imanol Agüero, Matías Tellado, Ana María Eiján, Betiana Lerner and Maximiliano Pérez
J. Imaging 2024, 10(11), 295; https://doi.org/10.3390/jimaging10110295 - 20 Nov 2024
Viewed by 167
Abstract
Sphere formation assay is an accepted cancer stem cell (CSC) enrichment method. CSCs play a crucial role in chemoresistance and cancer recurrence. Therefore, CSC growth is studied in plates and microdevices to develop prediction chemotherapy assays in cancer. As counting spheres cultured in [...] Read more.
Sphere formation assay is an accepted cancer stem cell (CSC) enrichment method. CSCs play a crucial role in chemoresistance and cancer recurrence. Therefore, CSC growth is studied in plates and microdevices to develop prediction chemotherapy assays in cancer. As counting spheres cultured in devices is laborious, time-consuming, and operator-dependent, a computational program called the Automatic Quantification of Spheres Algorithm (ASQA) that detects, identifies, counts, and measures spheres automatically was developed. The algorithm and manual counts were compared, and there was no statistically significant difference (p = 0.167). The performance of the AQSA is better when the input image has a uniform background, whereas, with a nonuniform background, artifacts can be interpreted as spheres according to image characteristics. The areas of spheres derived from LN229 cells and CSCs from primary cultures were measured. For images with one sphere, area measurements obtained with the AQSA and SpheroidJ were compared, and there was no statistically significant difference between them (p = 0.173). Notably, the AQSA detects more than one sphere, compared to other approaches available in the literature, and computes the sphere area automatically, which enables the observation of treatment response in the sphere derived from the human glioblastoma LN229 cell line. In addition, the algorithm identifies spheres with numbers to identify each one over time. The AQSA analyzes many images in 0.3 s per image with a low computational cost, enabling laboratories from developing countries to perform sphere counts and area measurements without needing a powerful computer. Consequently, it can be a useful tool for automated CSC quantification from cancer cell lines, and it can be adjusted to quantify CSCs from primary culture cells. CSC-derived sphere detection is highly relevant as it avoids expensive treatments and unnecessary toxicity. Full article
(This article belongs to the Special Issue Advancements in Imaging Techniques for Detection of Cancer)
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Graphical abstract

Graphical abstract
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<p>Microfluidic device architecture. (<b>a</b>) The microfluidic device design comprises 6 channels. Each channel has an inlet, 5 chambers, and an outlet. (<b>b</b>) Microfluidic device top view with scale in cm. (<b>c</b>) Microfluidic device side view.</p>
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<p>Flow diagram of image analysis process. Schematic representation of AQSA performance in uniform (12-multiwell plate) and nonuniform (microfluidic chip) environments.</p>
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<p>CLAHE and Gaussian filtering effect. Image quality improvement applying CLAHE and Gaussian filter.</p>
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<p>Gabor filter set application. Parameters of Gabor filter set: <span class="html-italic">f</span> = [1/4, 1/6, 1/8], <span class="html-italic">s</span><span class="html-italic">i</span><span class="html-italic">z</span><span class="html-italic">e</span> = 21, 6 rotations: <span class="html-italic">θ</span> = [<math display="inline"><semantics> <mrow> <msup> <mrow> <mn>0</mn> </mrow> <mrow> <mi>o</mi> </mrow> </msup> <mo>,</mo> <mo> </mo> <msup> <mrow> <mn>30</mn> </mrow> <mrow> <mi>o</mi> </mrow> </msup> <mo>,</mo> <mo> </mo> <msup> <mrow> <mn>60</mn> </mrow> <mrow> <mi>o</mi> </mrow> </msup> <mo>,</mo> <mo> </mo> <msup> <mrow> <mn>90</mn> </mrow> <mrow> <mi>o</mi> </mrow> </msup> <mo>,</mo> <mo> </mo> <msup> <mrow> <mn>120</mn> </mrow> <mrow> <mi>o</mi> </mrow> </msup> <mo>,</mo> <mo> </mo> <msup> <mrow> <mn>150</mn> </mrow> <mrow> <mi>o</mi> </mrow> </msup> </mrow> </semantics></math>], and <span class="html-italic">γ</span> = 10, η = 0.5.</p>
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<p>Relation between average area and cell number. Representative 40× images of LN229 cell line. White arrow indicates cell position. Scale bar: 100 μm. Solid line represents linear regression line. While dashed lines are the boundaries of all possible straight lines.</p>
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<p>Sphere detection, identification, and quantification in 12-well plate. (<b>a</b>) Original 10× image. (<b>b</b>) Detected spheres are circled in green and numbered in red. U251 human glioblastoma cell line. Scale bar 200 μm. (<b>c</b>) Sphere number comparison between AQSA algorithm and manual count performed with a hundred 10× U251 human glioblastoma cell line images. Student’s <span class="html-italic">t</span>-test (<span class="html-italic">p</span> = 0.167); ns means no statistically significant difference (<span class="html-italic">p</span> &gt; 0.05).</p>
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<p>Area quantification. (<b>a</b>) Area measurement comparison among ImageJ, manual, and AQSA for images with more than one sphere. (<b>b</b>) Area measurement comparison between AQSA and SpheroidJ for images with one sphere (Student’s <span class="html-italic">t</span>-test (<span class="html-italic">p</span> = 0.173); ns means no statistically significant difference (<span class="html-italic">p</span> &gt; 0.05)).</p>
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<p>Treatment response. Upper panel shows an image of LN229 cell-line-derived sphere. (<b>a</b>) Original 40× image and (<b>b</b>) AQSA analyzed image. Lower panel indicates treatment response according to (<b>c</b>) sphere formation efficiency (SFE), (<b>d</b>) sphere area, and (<b>e</b>) sphere number. Scale bar corresponds to 50 μm (* <span class="html-italic">p</span> ≤ 0.05 and ** <span class="html-italic">p</span> ≤ 0.01).</p>
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<p>High-grade compact solid canine thyroid carcinoma. (<b>a</b>) 4× image of a tracked in time sphere inside a microfluidic device chamber at day 4. Scale 100 um. (<b>b</b>) 4× image of a tracked in time sphere inside a microfluidic device chamber at day 5. (<b>c</b>) 40× image of sphere tracking at day 4. (<b>d</b>) 40× image of sphere tracking at day 5. Scale bar 100 µm. (<b>e</b>) Original 10× image of sphere derived from thyroid cancer cells. (<b>f</b>) Detected spheres reported by AQSA. Scale bar 100 µm. (<b>g</b>) Sphere manual count compared to AQSA count at days 3–5. (<b>h</b>) Area measurement by AQSA at different cell concentrations at days 3–5. White arrows indicate the tracking of the same sphere during time and ns means no statistically significant difference (<span class="html-italic">p</span> &gt; 0.05).</p>
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<p>Pleomorphic neoplastic lesion with carcinomatous pattern in the canine nasal cavity. (<b>a</b>) 4× image of a tracked in time sphere inside a microfluidic device chamber on day 10. Scale: 100 µm. (<b>b</b>) 40× image of sphere tracking day 10. (<b>c</b>) 40× image of sphere tracking day 12. Scale bar 50 µm. (<b>d</b>) Original 10× image of sphere derived from nasal tumor cells. (<b>e</b>) Detected spheres reported by AQSA. Scale bar 100 µm. (<b>f</b>) Sphere manual count compared to AQSA count on day 5 and day 10. (<b>g</b>) Area measurement by AQSA. White arrows indicate the tracking of the same sphere during time, ns means no statistically significant difference (<span class="html-italic">p</span> &gt; 0.05), and green circles mark the counted spheres.</p>
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<p>Comparison of AQSA sphere detection in two microscopes. Upper panel shows images acquired with phase-contrast Nikon microscope, and lower panel presents images obtained with an inverted Zeiss microscope. Upper panel 4× 12-well plate image scale bar corresponds to 500 µm, while 10× microdevice image scale bar corresponds to 200 µm. Lower panel 40× 12-well plate image scale bar corresponds to 50 µm, while 10× microdevice image scale bar corresponds to 100 µm.</p>
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20 pages, 4602 KiB  
Article
Low-Cost Solution for Air Quality Monitoring: Unmanned Aerial System and Data Transmission via LoRa Protocol
by Francisco David Parra-Medina, Manuel Andrés Vélez-Guerrero and Mauro Callejas-Cuervo
Sustainability 2024, 16(22), 10108; https://doi.org/10.3390/su162210108 - 20 Nov 2024
Viewed by 197
Abstract
For both human health and the environment, air pollution is a serious concern. However, the available air quality monitoring networks have important limitations, such as the high implementation costs, limited portability, and considerable operational complexity. In this context, unmanned aerial systems (UASs) are [...] Read more.
For both human health and the environment, air pollution is a serious concern. However, the available air quality monitoring networks have important limitations, such as the high implementation costs, limited portability, and considerable operational complexity. In this context, unmanned aerial systems (UASs) are emerging as a useful technological alternative due to their ability to cover large distances and access areas that are difficult or impossible for humans to reach. This article presents the development of an integrated platform that combines an unmanned aerial system (UAS) with specialized sensors to measure key parameters in relation to air quality, such as carbon monoxide (CO), ozone (O3), and nitrogen dioxide (NO2). In addition, a web application called PTECA is developed to visualize the data gathered by the wireless sensor array in real time. The platform incorporates a system that allows real-time tracking of the UAS route and measurement values during sample collection, employing the LoRa communication protocol. This solution represents a low-cost alternative that mitigates some of the limitations of traditional monitoring networks by offering greater portability and accessibility in terms of data collection. Preliminary tests successfully demonstrate the viability of the proposed system in a controlled airspace using geofencing. Full article
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<p>(<b>a</b>) Implementation of the general architecture of the described platform, including its hardware and software components, distributed in an aerial system and a ground system. (<b>b</b>) Block diagram showing the logical connection between its components.</p>
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<p>(<b>a</b>) Frontal view of the implemented UAS, showing the battery pack, propellers, landing gear, and electronic speed controllers (ESCs). (<b>b</b>) Top view of the implemented UAS, without the propellers and battery pack, allowing for a clear view of the APM flight controller module.</p>
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<p>(<b>a</b>) Sensor payload arrangement, suspended by a one-meter ribbon cable. (<b>b</b>) Payload components: GPS A1035, MQ7 (CO), MQ131 (O<sub>3</sub>), and MICS6814 (NO<sub>2</sub>).</p>
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<p>(<b>a</b>) General architecture and communication process of the PTECA application. (<b>b</b>) Application deployment process.</p>
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<p>(<b>a</b>) Outdoor UAS assembly and test, following the established design and operation parameters. (<b>b</b>) Flight protocol and platform data collection.</p>
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<p>Open field test with the aerial system on the ground, verifying the operation of the platform before the sampling flight.</p>
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<p>(<b>a</b>) Behavior of air quality measurements in the selected area. (<b>b</b>) Map of the UAV trajectory with the GPS waypoints, showing the location and capture time during sampling.</p>
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<p>(<b>a</b>) Behavior of air quality measurements in the selected area. (<b>b</b>) Map of the UAV trajectory with the GPS waypoints, showing the location and capture time during sampling.</p>
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18 pages, 3270 KiB  
Article
Long-Term Hydropower Plant Scheduling Considering Environmental and Economic Criteria
by Tatiana Myateg, Sergey Mitrofanov, Chen Xi, Yuri Sekretarev, Murodbek Safaraliev, Roman Volosatov, Anna Arestova and Aminjon Gulakhmadov
Sustainability 2024, 16(22), 10106; https://doi.org/10.3390/su162210106 - 19 Nov 2024
Viewed by 265
Abstract
This article is devoted to planning water-energy regimes for hydropower plants, taking into account economic and ecologic criteria. A new methodology based on a probabilistic model of water inflow has been proposed. The probabilistic method requires the calculation of low-water and average-water year [...] Read more.
This article is devoted to planning water-energy regimes for hydropower plants, taking into account economic and ecologic criteria. A new methodology based on a probabilistic model of water inflow has been proposed. The probabilistic method requires the calculation of low-water and average-water year typical hydrographs based on the probability curve. This allows the determination of the guaranteed hydropower plant generation schedule with a month time-step. According to the method considered, the mathematical model of the reservoir filling and normal power station operation has been designed. The software for the automated water-energy mode calculation is presented in this paper. The economic feasibility of maximum replacement of thermal power plants in the energy system with more environmentally friendly hydropower plant is substantiated. The methodology of water resources cost calculation and economic efficiency assessment under various hydropower plant regime scenarios have been proposed in the paper. Using the data and characteristics of HPPs and TPPs, an assessment of energy efficiency will be obtained in accordance with the developed methodology to determine the price of water for HPPs and all participants in the water management complex. The results of the implementation of the developed approach indicate that the price of electricity sales in a competitive electricity market can be brought into line with the price of electricity sales generated by thermal power plants, which increases the economic feasibility of the maximum replacement of thermal power plant capacity in the system with more economical and environmentally friendly hydropower plant. The developed method allows for an increase in the efficiency of water resources use and the efficiency of hydropower plant participation in the energy balance, which makes it possible to displace part of the power generated by thermal power plants. Full article
(This article belongs to the Section Environmental Sustainability and Applications)
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<p>Dependence of downstream level on water discharge.</p>
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<p>Reservoir drawdown/fill calculation program interface.</p>
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<p>Low-water year drawdown/fill schedule.</p>
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<p>Average water year drawdown/fill schedule.</p>
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<p>Graph of profit maximization when marginal revenue equals marginal cost (<span class="html-italic">MR</span>—marginal revenue, <span class="html-italic">MC</span>—marginal cost, <span class="html-italic">D</span>—market demand).</p>
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<p>Graph of profit maximization when marginal revenue equals marginal cost (<span class="html-italic">MR</span>—marginal revenue (blue line), <span class="html-italic">MC</span>—marginal cost (green line), <span class="html-italic">D</span>—market demand (red line)).</p>
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26 pages, 10461 KiB  
Article
Accuracy and Precision of Shallow-Water Photogrammetry from the Sea Surface
by Elisa Casella, Giovanni Scicchitano and Alessio Rovere
Remote Sens. 2024, 16(22), 4321; https://doi.org/10.3390/rs16224321 - 19 Nov 2024
Viewed by 339
Abstract
Mapping shallow-water bathymetry and morphology represents a technical challenge. In fact, acoustic surveys are limited by water depths reachable by boat, and airborne surveys have high costs. Photogrammetric approaches (either via drone or from the sea surface) have opened up the possibility to [...] Read more.
Mapping shallow-water bathymetry and morphology represents a technical challenge. In fact, acoustic surveys are limited by water depths reachable by boat, and airborne surveys have high costs. Photogrammetric approaches (either via drone or from the sea surface) have opened up the possibility to perform shallow-water surveys easily and at accessible costs. This work presents a simple, low-cost, and highly portable platform that allows gathering sequential photos and echosounder depth values of shallow-water sites (up to 5 m depth). The photos are then analysed in conjunction with photogrammetric techniques to obtain digital bathymetric models and orthomosaics of the seafloor. The workflow was tested on four repeated surveys of the same area in the Western Mediterranean and allowed obtaining digital bathymetric models with centimetric average accuracy and precision and root mean square errors within a few decimetres. The platform presented in this work can be employed to obtain first-order bathymetric products, enabling the contextual establishment of the depth accuracy of the final products. Full article
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<p>(<b>A</b>) Map of the Italian Peninsula. The star indicates the study site. Site where the test area (dashed line) is located as seen in: (<b>B</b>) orthomosaic of the area (Background image from Google Earth, 2022) and (<b>C</b>) oblique drone photo.</p>
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<p>Field setup used in this study. An operator working in snorkelling is dragging a diver’s buoy on top of which are fixed a dry case with a GNSS receiver (1) and a mobile phone (2). Fixed on the underwater part of the diver’s buoy are located a GoPro camera (3) and a portable echosounder (4). See text for details. The drawing is not to scale.</p>
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<p>Example of results obtained using the workflow outlined in the main text. (<b>A</b>) grid pattern followed by the snorkelling operator. (<b>B</b>) Orthomosaic (with hillshade in the background). (<b>C</b>) Digital bathymetric model (DBM) and echosounder points. Panels A, B, and C refer to the survey performed on the 13 August 2020. The same results for all surveys are shown in <a href="#remotesensing-16-04321-f0A2" class="html-fig">Figure A2</a>. (<b>D</b>–<b>G</b>) show an example of a picture for each survey date. The location pin (also shown in panel B) helps orient the image and place it in the reconstructed scene.</p>
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<p>Percentage of points and corresponding confidence calculated by Agisoft Metashape. Note that the surveys of 28 July and 13 August have higher confidence than the other two surveys, for which fewer photos were aligned by the program.</p>
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<p>Histograms showing the depth differences between DBM depths and control echosounder points (that represent the accuracy of each DBM), with average difference and RMSE for each survey date (panels <b>A</b>–<b>D</b>). For a plot of echosounder depths versus DBM depths, see <a href="#remotesensing-16-04321-f0A4" class="html-fig">Figure A4</a>.</p>
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<p>(<b>A</b>) Screenshot of the echosounder during data collection. The upper part shows the map location, while the lower part shows the sonogram surveyed by the echosounder. (<b>B</b>) Picture of the GNSS screen. This data is needed to syncronise the pictures taken with the GoPro camera with GNSS time.</p>
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<p>Same as in <a href="#remotesensing-16-04321-f003" class="html-fig">Figure 3</a>, but for all survey dates. The orthomosaics and DBMs shown here are not aligned to the 13 August one.</p>
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<p>Heatmap showing the RMSE between echosounder control points and DBM depths divided by survey date and depth bin. Darker blue colors represent higher RMSE.</p>
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<p>Scatterplots of DBM depths (x-axis) versus echosounder points depth (y-axis) for each survey date (panels <b>A</b>–<b>D</b>).</p>
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<p>Maps of the differences between DBMs from surveys performed on different dates.</p>
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<p>Histograms showing the differences between DBMs from surveys performed on different dates.</p>
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