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21 pages, 1550 KiB  
Article
Using 3D Hand Pose Data in Recognizing Human–Object Interaction and User Identification for Extended Reality Systems
by Danish Hamid, Muhammad Ehatisham Ul Haq, Amanullah Yasin, Fiza Murtaza and Muhammad Awais Azam
Information 2024, 15(10), 629; https://doi.org/10.3390/info15100629 - 12 Oct 2024
Viewed by 714
Abstract
Object detection and action/gesture recognition have become imperative in security and surveillance fields, finding extensive applications in everyday life. Advancement in such technologies will help in furthering cybersecurity and extended reality systems through the accurate identification of users and their interactions, which plays [...] Read more.
Object detection and action/gesture recognition have become imperative in security and surveillance fields, finding extensive applications in everyday life. Advancement in such technologies will help in furthering cybersecurity and extended reality systems through the accurate identification of users and their interactions, which plays a pivotal role in the security management of an entity and providing an immersive experience. Essentially, it enables the identification of human–object interaction to track actions and behaviors along with user identification. Yet, it is performed by traditional camera-based methods with high difficulties and challenges since occlusion, different camera viewpoints, and background noise lead to significant appearance variation. Deep learning techniques also demand large and labeled datasets and a large amount of computational power. In this paper, a novel approach to the recognition of human–object interactions and the identification of interacting users is proposed, based on three-dimensional hand pose data from an egocentric camera view. A multistage approach that integrates object detection with interaction recognition and user identification using the data from hand joints and vertices is proposed. Our approach uses a statistical attribute-based model for feature extraction and representation. The proposed technique is tested on the HOI4D dataset using the XGBoost classifier, achieving an average F1-score of 81% for human–object interaction and an average F1-score of 80% for user identification, hence proving to be effective. This technique is mostly targeted for extended reality systems, as proper interaction recognition and users identification are the keys to keeping systems secure and personalized. Its relevance extends into cybersecurity, augmented reality, virtual reality, and human–robot interactions, offering a potent solution for security enhancement along with enhancing interactivity in such systems. Full article
(This article belongs to the Special Issue Extended Reality and Cybersecurity)
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<p>Multi-stage HOI recognition.</p>
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<p>Representation of set of 21 3D hand Landmarks and vertices.</p>
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<p>Confusion matrix for object recognition (hand joints).</p>
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<p>Confusion matrix for object recognition (aand vertices).</p>
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<p>Confusion matrix for object recognition (fusion concatenation).</p>
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<p>Object based F1-Score for interaction classification.</p>
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<p>User identification average F1-Score in object-wise interactions.</p>
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15 pages, 3502 KiB  
Article
Evaluation of Haptic Textures for Tangible Interfaces for the Tactile Internet
by Nikolaos Tzimos, George Voutsakelis, Sotirios Kontogiannis and Georgios Kokkonis
Electronics 2024, 13(18), 3775; https://doi.org/10.3390/electronics13183775 - 23 Sep 2024
Viewed by 715
Abstract
Every texture in the real world provides us with the essential information to identify the physical characteristics of real objects. In addition to sight, humans use the sense of touch to explore their environment. Through haptic interaction we obtain unique and distinct information [...] Read more.
Every texture in the real world provides us with the essential information to identify the physical characteristics of real objects. In addition to sight, humans use the sense of touch to explore their environment. Through haptic interaction we obtain unique and distinct information about the texture and the shape of objects. In this paper, we enhance X3D 3D graphics files with haptic features to create 3D objects with haptic feedback. We propose haptic attributes such as static and dynamic friction, stiffness, and maximum altitude that provide the optimal user experience in a virtual haptic environment. After numerous optimization attempts on the haptic textures, we propose various haptic geometrical textures for creating a virtual 3D haptic environment for the tactile Internet. These tangible geometrical textures can be attached to any geometric shape, enhancing the haptic sense. We conducted a study of user interaction with a virtual environment consisting of 3D objects enhanced with haptic textures to evaluate performance and user experience. The goal is to evaluate the realism and recognition accuracy of each generated texture. The findings of the study aid visually impaired individuals to better understand their physical environment, using haptic devices in conjunction with the enhanced haptic textures. Full article
(This article belongs to the Section Computer Science & Engineering)
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<p>The nine texture patterns we study, representing nine categories of tests.</p>
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<p>(<b>a</b>) The Touch device and a computer monitor. (<b>b</b>) A pattern for the training phase with different scales.</p>
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<p>Experimental setup for haptic pattern texture evaluation without visual graphical textures of the haptic texture (<b>a</b>) and with visual graphical textures of the haptic texture (<b>b</b>).</p>
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<p>Example 4 of the 36 different pattern combinations.</p>
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<p>The seven phases of the experiment.</p>
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<p>Average score for uniformity detection and pattern recognition.</p>
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<p>(<b>a</b>) User-friendliness of haptic device. (<b>b</b>) More interaction time.</p>
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<p>Rate1, Rate2, and Rate3.</p>
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29 pages, 521 KiB  
Review
A Survey on the Use of Large Language Models (LLMs) in Fake News
by Eleftheria Papageorgiou, Christos Chronis, Iraklis Varlamis and Yassine Himeur
Future Internet 2024, 16(8), 298; https://doi.org/10.3390/fi16080298 - 19 Aug 2024
Cited by 1 | Viewed by 7303
Abstract
The proliferation of fake news and fake profiles on social media platforms poses significant threats to information integrity and societal trust. Traditional detection methods, including rule-based approaches, metadata analysis, and human fact-checking, have been employed to combat disinformation, but these methods often fall [...] Read more.
The proliferation of fake news and fake profiles on social media platforms poses significant threats to information integrity and societal trust. Traditional detection methods, including rule-based approaches, metadata analysis, and human fact-checking, have been employed to combat disinformation, but these methods often fall short in the face of increasingly sophisticated fake content. This review article explores the emerging role of Large Language Models (LLMs) in enhancing the detection of fake news and fake profiles. We provide a comprehensive overview of the nature and spread of disinformation, followed by an examination of existing detection methodologies. The article delves into the capabilities of LLMs in generating both fake news and fake profiles, highlighting their dual role as both a tool for disinformation and a powerful means of detection. We discuss the various applications of LLMs in text classification, fact-checking, verification, and contextual analysis, demonstrating how these models surpass traditional methods in accuracy and efficiency. Additionally, the article covers LLM-based detection of fake profiles through profile attribute analysis, network analysis, and behavior pattern recognition. Through comparative analysis, we showcase the advantages of LLMs over conventional techniques and present case studies that illustrate practical applications. Despite their potential, LLMs face challenges such as computational demands and ethical concerns, which we discuss in more detail. The review concludes with future directions for research and development in LLM-based fake news and fake profile detection, underscoring the importance of continued innovation to safeguard the authenticity of online information. Full article
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<p>Flowchart illustrating how papers were systematically selected for the survey.</p>
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<p>Retrieved publications per year.</p>
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<p>Number of publications per type.</p>
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<p>The most popular terms in the titles of the retrieved articles. Higher term count values correspond to a bigger size of the corresponding bubble and a lighter color.</p>
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25 pages, 2254 KiB  
Article
Exposing Data Leakage in Wi-Fi CSI-Based Human Action Recognition: A Critical Analysis
by Domonkos Varga
Inventions 2024, 9(4), 90; https://doi.org/10.3390/inventions9040090 - 15 Aug 2024
Viewed by 1153
Abstract
Wi-Fi channel state information (CSI)-based human action recognition systems have garnered significant interest for their non-intrusive monitoring capabilities. However, the integrity of these systems can be compromised by data leakage, particularly when improper dataset partitioning strategies are employed. This paper investigates the presence [...] Read more.
Wi-Fi channel state information (CSI)-based human action recognition systems have garnered significant interest for their non-intrusive monitoring capabilities. However, the integrity of these systems can be compromised by data leakage, particularly when improper dataset partitioning strategies are employed. This paper investigates the presence and impact of data leakage in three published Wi-Fi CSI-based human action recognition methods that utilize deep learning techniques. The original studies achieve precision rates of 95% or higher, attributed to the lack of human-based dataset splitting. By re-evaluating these systems with proper subject-based partitioning, our analysis reveals a substantial decline in performance, underscoring the prevalence of data leakage. This study highlights the critical need for rigorous dataset management and evaluation protocols to ensure the development of robust and reliable human action recognition systems. Our findings advocate for standardized practices in dataset partitioning to mitigate data leakage and enhance the generalizability of Wi-Fi CSI-based models. Full article
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<p>The general workflow of the method proposed by Jiao and Zhang [<a href="#B67-inventions-09-00090" class="html-bibr">67</a>]. First, CSI signals are converted into images, which are then analyzed by a CNN to predict human actions, using Gramian angular fields.</p>
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<p>Illustration of GASF and GADF computation. (<b>a</b>) Example time series. (<b>b</b>) Normalized time signal obtained using Equation (<a href="#FD3-inventions-09-00090" class="html-disp-formula">3</a>). (<b>c</b>) Mapping to polar coordinates using Equation (<a href="#FD4-inventions-09-00090" class="html-disp-formula">4</a>). (<b>d</b>) GASF obtained using Equation (<a href="#FD6-inventions-09-00090" class="html-disp-formula">6</a>). (<b>e</b>) GADF obtained using Equation (<a href="#FD8-inventions-09-00090" class="html-disp-formula">8</a>).</p>
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<p>Illustration of CSI signal conversion to RGB image applied in the method of Jiao and Zhang [<a href="#B67-inventions-09-00090" class="html-bibr">67</a>]. (<b>a</b>) Raw CSI signal. (<b>b</b>) Filtered CSI signal. (<b>c</b>) GASF. (<b>d</b>) GADF.</p>
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<p>Structure of the CNN proposed and implemented by Jiao and Zhang [<a href="#B67-inventions-09-00090" class="html-bibr">67</a>] for Wi-Fi CSI-based HAR. Batch normalization layers were implemented after each convolutional layer to increase convergence speed, followed by ReLU as activation functions.</p>
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<p>The general workflow of the Wi-Fi CSI-based HAR method proposed by Jawad and Alaziz [<a href="#B75-inventions-09-00090" class="html-bibr">75</a>].</p>
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<p>Illustration of CSI signal conversion to RGB image applied in Jawad et al.’s [<a href="#B75-inventions-09-00090" class="html-bibr">75</a>] method: (<b>a</b>) 30 Hampel filtered CSI signals. (<b>b</b>) CSI signals converted to RGB image using MATLAB’s <span class="html-italic">imagesc</span> function.</p>
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<p>Structure of the CNN proposed and implemented by Shahverdi et al. [<a href="#B81-inventions-09-00090" class="html-bibr">81</a>] for Wi-Fi CSI-based HAR. To avoid overfitting, the authors implemented dropout with <math display="inline"><semantics> <mrow> <mi>p</mi> <mo>=</mo> <mn>0.25</mn> </mrow> </semantics></math> parameter [<a href="#B84-inventions-09-00090" class="html-bibr">84</a>] after each convolutional and dense layer. Further, batch normalization layers were also implemented after each convolutional layer to further reduce overfitting. The authors used leaky ReLU [<a href="#B85-inventions-09-00090" class="html-bibr">85</a>] as the activation function.</p>
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<p>Training of ResNet50 on WiAR [<a href="#B88-inventions-09-00090" class="html-bibr">88</a>] without respect to humans. In the upper figure, the training accuracy is represented by the blue line, whereas the validation accuracy is depicted in black. In the lower figure, the training loss is indicated in red, and the validation loss is shown in black.</p>
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<p>Training of ResNet50 on WiAR [<a href="#B88-inventions-09-00090" class="html-bibr">88</a>] with respect to humans. In the upper figure, the training accuracy is represented by the blue line, whereas the validation accuracy is depicted in black. In the lower figure, the training loss is indicated in red, and the validation loss is shown in black.</p>
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<p>Confusion matrices of fine-tuned ResNet50 obtained on WiAR [<a href="#B88-inventions-09-00090" class="html-bibr">88</a>] test set. (<b>a</b>) Results obtained from retraining without respect to humans. (<b>b</b>) Results obtained from retraining with respect to humans.</p>
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<p>Confusion matrices of fine-tuned ResNet50 obtained on WiAR [<a href="#B88-inventions-09-00090" class="html-bibr">88</a>] test set. (<b>a</b>) Results obtained from retraining without respect to humans. (<b>b</b>) Results obtained from retraining with respect to humans.</p>
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<p>Illustration of RGB images in the CSI-HAR database [<a href="#B90-inventions-09-00090" class="html-bibr">90</a>]. (<b>a</b>) Run. (<b>b</b>) Sit down. (<b>c</b>) Stand up. (<b>d</b>) Walk.</p>
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<p>Confusion matrices of the deep architecture (RGB CSI images as input) proposed by Shahverdi et al. [<a href="#B81-inventions-09-00090" class="html-bibr">81</a>] obtained on CSI-HAR [<a href="#B90-inventions-09-00090" class="html-bibr">90</a>] test set. (<b>a</b>) Results obtained from retraining without respect to humans. (<b>b</b>) Results obtained from retraining with respect to humans.</p>
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<p>Training of CNN architecture proposed by Shahverdi et al. [<a href="#B81-inventions-09-00090" class="html-bibr">81</a>] without respect to humans on CSI-HAR [<a href="#B90-inventions-09-00090" class="html-bibr">90</a>] dataset. In the upper figure, the training accuracy is represented by the blue line, whereas the test accuracy is depicted in black. In the lower figure, the training loss is indicated in red, and the test loss is shown in black.</p>
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<p>Training of CNN architecture proposed by Shahverdi et al. [<a href="#B81-inventions-09-00090" class="html-bibr">81</a>] with respect to humans on CSI-HAR [<a href="#B90-inventions-09-00090" class="html-bibr">90</a>] dataset. In the upper figure, the training accuracy is represented by the blue line, whereas the test accuracy is depicted in black. In the lower figure, the training loss is indicated in red, and the test loss is shown in black.</p>
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19 pages, 12382 KiB  
Article
Mapping the Functional Structure of Urban Agglomerations at the Block Level: A New Spatial Classification That Goes beyond Land Use
by Bin Ai, Zhenlin Lai and Shifa Ma
Land 2024, 13(8), 1148; https://doi.org/10.3390/land13081148 - 26 Jul 2024
Viewed by 560
Abstract
The functional structure of territorial space is an important factor for analyzing the interaction between humans and nature. However, the classification of remote sensing images struggles to distinguish between multiple functions provided by the same land use type. Therefore, we propose a framework [...] Read more.
The functional structure of territorial space is an important factor for analyzing the interaction between humans and nature. However, the classification of remote sensing images struggles to distinguish between multiple functions provided by the same land use type. Therefore, we propose a framework to combine multi-source data for the recognition of dominant functions at the block level. Taking the Guangdong–Hong Kong–Macau Greater Bay Area (GBA) as a case study, its block-level ‘production–living–ecology’ functions were interpreted. The whole GBA was first divided into different blocks and its total, average, and proportional functional intensities were then calculated. Each block was labeled as a functional type considering the attributes of human activity and social information. The results show that the combination of land use/cover data, point of interest identification, and open street maps can efficiently separate the multiple and mixed functions of the same land use types. There is a great difference in the dominant functions of the cities in the GBA, and the spatial heterogeneity of their mixed functions is closely related to the development of their land resources and socio-economy. This provides a new perspective for recognizing the spatial structure of territorial space and can give important data for regulating and optimizing landscape patterns during sustainable development. Full article
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<p>Technical flowchart for identifying and analyzing PLE functions at the block level.</p>
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<p>Scope of the study area, land use in 2020, and divided blocks: (<b>a</b>) the river is not separated to maintain its integrity; (<b>b</b>) blocks containing natural elements; and (<b>c</b>) blocks within urban areas.</p>
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<p>Total, average, and proportional function intensity of different PLE function units.</p>
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<p>Identification principle for determining the function type of blocks in the GBA.</p>
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<p>Distribution pattern of different dominant function types in the GBA, where (<b>a</b>–<b>h</b>) are the local spatial distribution in Zhaoqing, western Guangdong, southern Guangzhou, Foshan, Shenzhen, Jiangmen, Zhongshan, Hong Kong.</p>
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<p>Accuracy validation of PLE identification by comparison of high-resolution images and street view map, where ①–④ are examples of PLE identification results in this study.</p>
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<p>Quantity structure of dominant function types in different cities of the GBA.</p>
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<p>Proportions of POIs for different PLE functions (Logo icons demonstrate representative annotation points for different types of POIs).</p>
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<p>Typical cases of identified PLE blocks overlaid with high-resolution imagery, including residential communities (<b>A</b>,<b>C</b>,<b>E</b>) an ecological space (<b>B</b>) and a production space (<b>D</b>).</p>
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<p>Comparison between PLE identification result and classification product. (<b>a</b>) Examples of PLE identification results in this study. (<b>b</b>) ESA land cover product at 10 m resolution. (<b>c</b>) Google Earth images. ①–④ are the examples for the comparison.</p>
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15 pages, 233 KiB  
Article
No Animal Left Behind: A Thematic Analysis of Public Submissions on the New Zealand Emergency Management Bill
by Steve Glassey
Pets 2024, 1(2), 120-134; https://doi.org/10.3390/pets1020010 - 11 Jul 2024
Viewed by 1024
Abstract
This article presents a thematic analysis of submissions made on New Zealand’s Emergency Management Bill. While a key focus is on the importance and frequency of animal welfare concerns raised by submitters, the analysis also examines other critical themes to provide context on [...] Read more.
This article presents a thematic analysis of submissions made on New Zealand’s Emergency Management Bill. While a key focus is on the importance and frequency of animal welfare concerns raised by submitters, the analysis also examines other critical themes to provide context on the range of issues addressed. The impact of the “No Animal Left Behind” campaign launched by Animal Evac New Zealand in mobilising public engagement on animal welfare provisions is also assessed. Sixty-one percent (n = 191) of public submissions on the Bill raised the importance of including animals in new emergency management legislation and at least 48% (n = 149) of all public submissions were directly attributed to the campaign. Key animal welfare concerns include the need for clear statutory powers and requirements, better coordination and resourcing, and recognition of the human–animal bond. Other prominent non-animal-related themes relate to strengthening community resilience, improving Māori participation in emergency management, and enhancing readiness and response capabilities. Specific recommendations are made for legal changes to better protect animal welfare, including amending key provisions to explicitly address animal rescue and evacuation, mandating animal welfare emergency plans, strengthening animal seizure and disposal processes, and enhancing accountability for animal emergency response charities. With improving animal disaster management law being the most common issue identified, it is logical for a government to apply deliberative democracy to ensure animals are better protected in New Zealand emergency management reforms. The findings underscore the importance of comprehensive, multi-faceted reform to create a world-leading emergency management framework. Full article
12 pages, 297 KiB  
Article
Interdependency and Change: God in the Chinese Theology of Xie Fuya (1892–1991)
by Kenpa Chin
Religions 2024, 15(6), 687; https://doi.org/10.3390/rel15060687 - 31 May 2024
Viewed by 932
Abstract
Xie Fuya (N. Z. Zia, 1892–1991), a major Chinese Christian thinker, has contributed much to the development of Sino-theology. However, his work has yet to receive the recognition it deserves. As a thinker who is well-versed in both Chinese and Western philosophies while [...] Read more.
Xie Fuya (N. Z. Zia, 1892–1991), a major Chinese Christian thinker, has contributed much to the development of Sino-theology. However, his work has yet to receive the recognition it deserves. As a thinker who is well-versed in both Chinese and Western philosophies while dedicating himself to the exploration of the philosophy of religion, Xie presents a dual feature in his writings. On the one hand, his work engages in a dialogical discourse between Eastern and Western philosophies. On the other hand, his writings represent an ambitious attempt to interpret traditional Chinese philosophical tenets within the context of Christian theology, transverse from the level of human nature to the level of ontological existence, representing an innovative model of contemplation in the field of Sino-theology. This contribution is of immense value to the development of Chinese philosophical thought. For this reason, this article attempts to illustrate, through Xie’s writings in various stages of his life, his relentless effort to promote the integration of Eastern and Western philosophies within the framework of Chinese thought. His most notable accomplishment in this East–West confluence effort is his unique assumption of God’s attributions as both zhonghe (literally “middle harmony”, connoted as interdependency by Xie) and bianyi (change). Full article
(This article belongs to the Special Issue History and Theology of Chinese Christianity)
13 pages, 729 KiB  
Review
Hepatocyte Intrinsic Innate Antiviral Immunity against Hepatitis Delta Virus Infection: The Voices of Bona Fide Human Hepatocytes
by Yein Woo, Muyuan Ma, Masashi Okawa and Takeshi Saito
Viruses 2024, 16(5), 740; https://doi.org/10.3390/v16050740 - 8 May 2024
Cited by 3 | Viewed by 2139
Abstract
The pathogenesis of viral infection is attributed to two folds: intrinsic cell death pathway activation due to the viral cytopathic effect, and immune-mediated extrinsic cellular injuries. The immune system, encompassing both innate and adaptive immunity, therefore acts as a double-edged sword in viral [...] Read more.
The pathogenesis of viral infection is attributed to two folds: intrinsic cell death pathway activation due to the viral cytopathic effect, and immune-mediated extrinsic cellular injuries. The immune system, encompassing both innate and adaptive immunity, therefore acts as a double-edged sword in viral infection. Insufficient potency permits pathogens to establish lifelong persistent infection and its consequences, while excessive activation leads to organ damage beyond its mission to control viral pathogens. The innate immune response serves as the front line of defense against viral infection, which is triggered through the recognition of viral products, referred to as pathogen-associated molecular patterns (PAMPs), by host cell pattern recognition receptors (PRRs). The PRRs–PAMPs interaction results in the induction of interferon-stimulated genes (ISGs) in infected cells, as well as the secretion of interferons (IFNs), to establish a tissue-wide antiviral state in an autocrine and paracrine manner. Cumulative evidence suggests significant variability in the expression patterns of PRRs, the induction potency of ISGs and IFNs, and the IFN response across different cell types and species. Hence, in our understanding of viral hepatitis pathogenesis, insights gained through hepatoma cell lines or murine-based experimental systems are uncertain in precisely recapitulating the innate antiviral response of genuine human hepatocytes. Accordingly, this review article aims to extract and summarize evidence made possible with bona fide human hepatocytes-based study tools, along with their clinical relevance and implications, as well as to identify the remaining gaps in knowledge for future investigations. Full article
(This article belongs to the Special Issue Life Cycle of Hepatitis D Virus (HDV) and HDV-Like Agents)
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<p>Overview of the hepatic IFN system in the regulation of HDV infection in human hepatocytes: current understanding and gaps in knowledge. HDV enters hepatocytes through the interaction between its HBsAg and the host cell surface protein NTCP. Replication occurs in the nucleus, generating various viral RNA species. Currently, MDA5, one of the RLHs, and to a lesser extent, RIG-I, are considered key PRRs that sense HDV PAMPs (vRNA species). The interaction between RLHs and vRNA species triggers the activation of the MAVS-IRF3/7 pathway and induces ISGs and IFNs (1). ADAR1, an ISG, facilitates the HDV life cycle by introducing a point mutation enabling L-HDAg production, functioning as a proviral host factor. OAS, another ISG, activates the RNaseL pathway via the production of ppp2′-5′A, which in turn produces RIG-I and MDA5 ligands through cleaving vRNA and host RNA species. Therefore, both RIG-I and MDA5 are expected to play a role in the induction of ISGs and IFNs in HDV infection. IFNs, predominantly type III IFNs, secreted from the infected hepatocytes act on both infected and infection-naïve hepatocytes to induce ISGs via activation of Jak-STAT signaling cascades (2); thereby serving as the second wave of the antiviral response in the infected cells as well as establishing a tissue-wide antiviral state in the liver. Despite these sophisticated innate antiviral responses, the hepatocyte intrinsic IFN system is incapable of halting HDV infection due to HDV’s high resistance to the antiviral properties of ISGs and the establishment of cellular IFN refractoriness resulting from constitutive exposure to IFNs.</p>
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42 pages, 122307 KiB  
Article
Toward Synthetic Physical Fingerprint Targets
by Laurenz Ruzicka, Bernhard Strobl, Stephan Bergmann, Gerd Nolden, Tom Michalsky, Christoph Domscheit, Jannis Priesnitz, Florian Blümel, Bernhard Kohn and Clemens Heitzinger
Sensors 2024, 24(9), 2847; https://doi.org/10.3390/s24092847 - 29 Apr 2024
Viewed by 1124
Abstract
Biometric fingerprint identification hinges on the reliability of its sensors; however, calibrating and standardizing these sensors poses significant challenges, particularly in regards to repeatability and data diversity. To tackle these issues, we propose methodologies for fabricating synthetic 3D fingerprint targets, or phantoms, that [...] Read more.
Biometric fingerprint identification hinges on the reliability of its sensors; however, calibrating and standardizing these sensors poses significant challenges, particularly in regards to repeatability and data diversity. To tackle these issues, we propose methodologies for fabricating synthetic 3D fingerprint targets, or phantoms, that closely emulate real human fingerprints. These phantoms enable the precise evaluation and validation of fingerprint sensors under controlled and repeatable conditions. Our research employs laser engraving, 3D printing, and CNC machining techniques, utilizing different materials. We assess the phantoms’ fidelity to synthetic fingerprint patterns, intra-class variability, and interoperability across different manufacturing methods. The findings demonstrate that a combination of laser engraving or CNC machining with silicone casting produces finger-like phantoms with high accuracy and consistency for rolled fingerprint recordings. For slap recordings, direct laser engraving of flat silicone targets excels, and in the contactless fingerprint sensor setting, 3D printing and silicone filling provide the most favorable attributes. Our work enables a comprehensive, method-independent comparison of various fabrication methodologies, offering a unique perspective on the strengths and weaknesses of each approach. This facilitates a broader understanding of fingerprint recognition system validation and performance assessment. Full article
(This article belongs to the Topic Applications in Image Analysis and Pattern Recognition)
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<p>Synthetic fingerprint generation with SFinGe output (<b>a</b>), applied Gabor filter (<b>b</b>), applied thresholding algorithm (<b>c</b>), and finally, path traced and converted to a vector graphic (<b>d</b>).</p>
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<p>Laser-engraved elastomer targets. (<b>a</b>) Laser-engraved elastomer stripes. Stripe with 0.95 mm thickness on top, stripe with 1.42 mm thickness below. (<b>b</b>) Exemplary elastomer stripes applied to the wooden target holder.</p>
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<p>Silicone plates used for laser engraving. (<b>a</b>) Silicone plate from the company Gospire used as a training skin for tattoo artists. (<b>b</b>) Silicone plate created in-house with Dragon Skin 10 Fast.</p>
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<p>Aluminum half pipe mold. (<b>a</b>) Aluminum half-pipe mold with laser engraving. (<b>b</b>) Plug for filling the mold with silicone.</p>
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<p>Resin-printed mold halves. (<b>a</b>) Printed using the ES2 Elegoo Saturn 2-8K resin printer. (<b>b</b>) Printed using the Alpine3D GmbH SLA service.</p>
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<p>Silicone target made from 3D-printed resin mold.</p>
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<p>CNC-machined master targets.</p>
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<p>Synthetic fingerprint results used for the following: laser-engraved elastomer samples (<b>a</b>), both laser-engraved silicone samples and the laser-engraved aluminum half-pipe mold (<b>b</b>), and the first version of the 3D-printed resin mold (<b>c</b>).</p>
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<p>Upsampling of synthetic fingerprint image used for 3D-printed second-generation resin mold.</p>
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<p>Laser-engraved phantoms (<b>b</b>–<b>e</b>) and mold (<b>a</b>). Phantom (<b>b</b>) is the silicone filling of the aluminum mold (<b>a</b>), and the other images are direct laser engraving on elastomer (<b>c</b>) and silicone (<b>d</b>,<b>e</b>).</p>
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<p>The figure shows the top-down view on the synthetic fingerprint engraved in the elastomer with the corresponding color coded height profile. Scale bar 5 mm.</p>
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<p>The figure shows the top-down view on the checkerboard structure engraved in the elastomer with the corresponding height profile color coded. The yellow/magenta line highlights the area of the height profile in the bottom plot, while the read dashed line indicates the same area in the height map. Within the plot, the increasing and decreasing shoulder and the upper and lower plateau of the structure were selected by hand. Scale bar 5 mm.</p>
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<p>Top-down capture of the Gospire silicone plate. The cyan/yellow line in the upper left image highlights the area where the height profile is measured and the red line the corresponding area in the height map. Scale bar 5 mm.</p>
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<p>Top-down capture of the in-house created Dragon Skin 10 Fast silicone plate. The cyan/yellow line in the upper left image highlights the area where the height profile is measured and the red line the corresponding area in the height map. Scale bar 5 mm.</p>
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<p>Top-down capture of an in-house Dragon Skin 10 Fast synthetic fingerprint sample created by casting the silicone in an aluminum half-pipe with laser-engraved fingerprint structure. The cyan/yellow line in the upper left image highlights the area where the height profile is measured and the red line the corresponding area in the height map. Scale bar 5 mm.</p>
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<p>3D-printed resin molds and fingerprint phantoms. Created with the ES2 Elegoo Saturn 2-8K resin printer (ES2) or the Alpine3D GmbH SLA service (ALP).</p>
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<p>Top-down captures of created Gelafix-based fingerprint targets on different days. From <b>left</b> to <b>right</b>, day 0, day 1, day 4, and after approximately 6 months. The corresponding diameters of the fitted cylinders are summarized in <a href="#sensors-24-02847-t002" class="html-table">Table 2</a>. Scale bar 5 mm.</p>
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<p>Top-down capture of a fresh Gelafix sample created using the in-house ES2 Elegoo Saturn 2-8K resin printer. The yellow/purple line highlights the area selected for measuring the height profile, which can be seen in the height profile via the dashed red line.</p>
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<p>Top-down capture of an approximate 6-month-old Gelafix sample created using the in-house ES2 Elegoo Saturn 2-8K resin printer. The cyan/yellow line, which can be seen in the height profile via the dashed red line. highlights the area selected for measuring the height profile.</p>
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<p>Finger phantom cast with Dragon Skin 10, while using the 3D resin-printed mold by Alpine 3D. The yellow/purple line highlights the area selected for measuring the height profile, which can be seen in the height profile via the dashed red line.</p>
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<p>Fingerprint phantoms made from CNC-machined aluminum master targets.</p>
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<p>Top-down captures of the Dragon Skin 10 Fast-based fingerprint targets with a concentric Ronchi pattern created from the CNC-machined aluminum master target. Along the yellow/cyan line, the height profile is measured, which can be seen in the height profile via the dashed red line.</p>
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<p>Dragon Skin 10 Fast-based fingerprint targets with a synthetic fingerprint created via a negative mold taken from the aluminum milled master targets. The height profile along the cyan/yellow line is taken to measure the ridge line depth and width, which can be seen in the height profile via the dashed red line.</p>
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17 pages, 7113 KiB  
Article
Effect of Face Masks on Automatic Speech Recognition Accuracy for Mandarin
by Xiaoya Li, Ke Ni and Yu Huang
Appl. Sci. 2024, 14(8), 3273; https://doi.org/10.3390/app14083273 - 12 Apr 2024
Cited by 1 | Viewed by 793
Abstract
Automatic speech recognition (ASR) has been widely used to realize daily human–machine interactions. Face masks have become everyday wear in our post-pandemic life, and speech through masks may have impaired the ASR. This study explored the effects of different kinds of face masks [...] Read more.
Automatic speech recognition (ASR) has been widely used to realize daily human–machine interactions. Face masks have become everyday wear in our post-pandemic life, and speech through masks may have impaired the ASR. This study explored the effects of different kinds of face masks (e.g., surgical mask, KN95 mask, and cloth mask) on the Mandarin word accuracy of two ASR systems with or without noises. A mouth simulator was used to play speech audio with or without wearing a mask. Acoustic signals were recorded at distances of 0.2 m and 0.6 m. Recordings were mixed with two noises at a signal-to-noise ratio of +3 dB: restaurant noise and speech-shaped noise. Results showed that masks did not affect ASR accuracy without noise. Under noises, masks did not significantly influence ASR accuracy at 0.2 m but had significant effects at 0.6 m. The activated-carbon mask had the most significant impact on ASR accuracy at 0.6 m, reducing the accuracy by 18.5 percentage points compared to that without a mask, whereas the cloth mask had the least effect on ASR accuracy at 0.6 m, reducing the accuracy by 0.9 percentage points. The acoustic attenuation of masks on the high-frequency band at around 3.15 kHz of the speech signal attributed to the effects of masks on ASR accuracy. When training ASR models, it may be important to consider mask robustness. Full article
(This article belongs to the Special Issue Signal Acquisition and Processing for Measurement and Testing)
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<p>Connection set-up of apparatus and 7 mask conditions. (<b>a</b>) Connection set-up of apparatus; (<b>b</b>) No mask (M0); (<b>c</b>) Surgical mask (M1); (<b>d</b>) Activated-carbon mask (M2); (<b>e</b>) Hanging-ear medical protective mask (M3); (<b>f</b>) Headwear medical protective mask (M4); (<b>g</b>) Anti-particulate mask (M5); and (<b>h</b>) Cloth mask (M6).</p>
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<p>The research procedure.</p>
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<p>The word accuracy (ACC) values for all speakers at a recording distance of 0.2 m. (<b>a</b>) ASR<sub>D</sub>, no noise; (<b>b</b>) ASR<sub>T</sub>, no noise; (<b>c</b>) ASR<sub>D</sub>, restaurant noise; (<b>d</b>) ASR<sub>T</sub>, restaurant noise; (<b>e</b>) ASR<sub>D</sub>, speech-shaped noise; and (<b>f</b>) ASR<sub>T</sub>, speech-shaped noise.</p>
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<p>The ACC for all speakers at a recording distance of 0.6 m. (<b>a</b>) ASR<sub>D</sub>, no noise; (<b>b</b>) ASR<sub>T</sub>, no noise; (<b>c</b>) ASR<sub>D</sub>, restaurant noise; (<b>d</b>) ASR<sub>T</sub>, restaurant noise; (<b>e</b>) ASR<sub>D</sub>, speech-shaped noise; and (<b>f</b>) ASR<sub>T</sub>, speech-shaped noise. Kruskal–Wallis tests and post-pairwise comparisons: *, <span class="html-italic">p</span> &lt; 0.05; **, <span class="html-italic">p</span> &lt; 0.01; and ***, <span class="html-italic">p</span> &lt; 0.001.</p>
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<p>The ACC for male speakers at a recording distance of 0.6 m. (<b>a</b>) ASR<sub>D</sub>, no noise; (<b>b</b>) ASR<sub>T</sub>, no noise; (<b>c</b>) ASR<sub>D</sub>, restaurant noise; (<b>d</b>) ASR<sub>T</sub>, restaurant noise; (<b>e</b>) ASR<sub>D</sub>, speech-shaped noise; and (<b>f</b>) ASR<sub>T</sub>, speech-shaped noise. Kruskal–Wallis tests and post-pairwise comparisons: *, <span class="html-italic">p</span> &lt; 0.05; **, <span class="html-italic">p</span> &lt; 0.01; ***, and <span class="html-italic">p</span> &lt; 0.001.</p>
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<p>The ACC for female speakers at a recording distance of 0.6 m. (<b>a</b>) ASR<sub>D</sub>, no noise; (<b>b</b>) ASR<sub>T</sub>, no noise; (<b>c</b>) ASR<sub>D</sub>, restaurant noise; (<b>d</b>) ASR<sub>T</sub>, restaurant noise; (<b>e</b>) ASR<sub>D</sub>, speech-shaped noise; (<b>f</b>) ASR<sub>T</sub>, speech-shaped noise. Kruskal–Wallis tests and post-pairwise comparisons: *, <span class="html-italic">p</span> &lt; 0.05; **, <span class="html-italic">p</span> &lt; 0.01.</p>
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<p>The sound-transmission loss of masks.</p>
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<p>The average spectra of the restaurant noise, the speech-shaped noise, and the speech material. The amplitude of the spectra was normalized.</p>
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16 pages, 22375 KiB  
Article
Identifying Residues for Substrate Recognition in Human GPAT4 by Molecular Dynamics Simulations
by Yulan Liu, Yunong Xu, Yinuo Xu, Zhihao Zhao, Gui-Juan Cheng, Ruobing Ren and Ying-Chih Chiang
Int. J. Mol. Sci. 2024, 25(7), 3729; https://doi.org/10.3390/ijms25073729 - 27 Mar 2024
Viewed by 1128
Abstract
Glycerol-3-phosphate acyltransferase (GPAT) catalyzes the first step in triacylglycerol synthesis. Understanding its substrate recognition mechanism may help to design drugs to regulate the production of glycerol lipids in cells. In this work, we investigate how the native substrate, glycerol-3-phosphate (G3P), and palmitoyl-coenzyme A [...] Read more.
Glycerol-3-phosphate acyltransferase (GPAT) catalyzes the first step in triacylglycerol synthesis. Understanding its substrate recognition mechanism may help to design drugs to regulate the production of glycerol lipids in cells. In this work, we investigate how the native substrate, glycerol-3-phosphate (G3P), and palmitoyl-coenzyme A (CoA) bind to the human GPAT isoform GPAT4 via molecular dynamics simulations (MD). As no experimentally resolved GPAT4 structure is available, the AlphaFold model is employed to construct the GPAT4–substrate complex model. Using another isoform, GPAT1, we demonstrate that once the ligand binding is properly addressed, the AlphaFold complex model can deliver similar results to the experimentally resolved structure in MD simulations. Following the validated protocol of complex construction, we perform MD simulations using the GPAT4–substrate complex. Our simulations reveal that R427 is an important residue in recognizing G3P via a stable salt bridge, but its motion can bring the ligand to different binding hotspots on GPAT4. Such high flexibility can be attributed to the flexible region that exists only on GPAT4 and not on GPAT1. Our study reveals the substrate recognition mechanism of GPAT4 and hence paves the way towards designing GPAT4 inhibitors. Full article
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<p>(<b>a</b>) Result of multiple sequence alignment. Listed sequences are Human GPAT1 to Human GPAT4, GPAT (PlsB) from <span class="html-italic">Escherichia coli</span>, GPAT (PlsB) from <span class="html-italic">Haemophilus influenzae</span>, GPAT1 from mouse and GPAT1 from rat. Motifs I, II, III, IV are highlighted in red, blue, yellow and orange, respectively. Notably, conserved residues (indicated by *) and conservative replacements (indicated by :) are clustered around motif I and motif IV. The semi-conservative replacements (indicated by .) are also labeled. (<b>b</b>) The phylogenetic tree of the aligned sequences shows their phylogenetic relatedness. Despite having evolved from a common ancestor, GPAT4 shares limited relatedness with GPAT1 and GPAT2 compared to GPAT3. The phylogenetic tree was constructed using the Maximum Likelihood method.</p>
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<p>Binding pockets of GPAT1 and GPAT4. (<b>a</b>) Structure alignment of GPAT1 (8E50, cyan) and GPAT4 (AlphaFold, mauve). The two proteins have a very similar binding pocket, as indicated by the associated secondary structures. (<b>b</b>) The binding pocket of GPAT1 (8E50) found by MOE-SiteFinder. The pocket depicted by the green transparent volume is a combination of one large cavity and five adjacent small cavities. Motifs I to IV are colored in red, blue, yellow and orange, respectively. Ligands are colored in green. (<b>c</b>) The largest binding pocket of GPAT4 (AlphaFold) identified by MOE-SiteFinder (green transparent volume). It coincides with GPAT1’s binding site, which is indicated by the ligands (green).</p>
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<p>Structures of motifs I to IV. In order, they are colored in red, blue, yellow and orange, respectively. Ligands are colored in green. The catalytic histidine is labeled by *. (<b>a</b>) GPAT1 structure taken from 8E50. Residue R320 clearly interacts with the phosphate group on G3P. (<b>b</b>) GPAT1 structure taken from the AlphaFold model. G3P and CoA are depicted for reference. The RMSD of the four motifs’ heavy atoms is 2.14 Å between 8E50 and the current model. Notably, R278 should interact with CoA as in 8E50, but it now appears within 4 Å of G3P. (<b>c</b>) GPAT4 structure taken from the AlphaFold model. G3P and CoA are depicted for reference. Unlike GPAT1, there is no arginine on motif III (yellow) to interact with G3P. The nearby ARG427 is not located on motif III.</p>
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<p>Results of the MD simulations using three different complex models: the complex modeled from PDB (8E50), the complex modeled from AlphaFold with ligands from PDB (AF+ligands) and the complex from AlphaFold with ligands from molecular docking (AF+docking). (<b>a</b>) The contact frequency with G3P obtained from MD trajectories. Important residues such as R318 and R320 have a high contact frequency over all three complex models. These two residues recognize the native substrate through the electrostatic interaction with the phosphate group on G3P. Panels (<b>b</b>–<b>d</b>) depict the first cluster centroid found in the MD simulations of model 8E50, model AF+ligands and model AF+docking, respectively. The former two have a more dominant cluster, viz. 97.5% and 99.1% of the trajectory length. MD simulations find no dominant ligand conformation in model AF+docking, where the largest cluster size is merely 29.2% of the trajectory. G3P is colored in green. CoA is shown in translucent green. Positively charged residues within 3 Å of G3P’s phosphate group are shown in yellow, while T317, N258 and H230 are colored in cyan. Residues R278 and K373 also appear within 3 Å of G3P in model AF+docking. For clearer visualization, these two residues are depicted in <a href="#app1-ijms-25-03729" class="html-app">Figure S9</a>.</p>
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<p>Results of MD simulations for the GPAT4 complex. (<b>a</b>) The contact frequency with G3P. R427 is the major residue interacting with G3P in all trajectories. Panels (<b>b</b>–<b>g</b>) depict the ligands’ centroid conformations of the first to the sixth cluster obtained from the simulations, respectively. The corresponding cluster sizes are 13.8%, 11.5%, 9.1%, 7.1%, 6.4%, 5.2% of the trajectory length, respectively. G3P is again colored in green. CoA is colored in translucent green. Positively charged residues within 3 Å of G3P’s phosphate group are colored in yellow. Other residues are colored in cyan. Residues 419 to 431 (loop in purple) show a large fluctuation over these clusters. (<b>h</b>) Transition between different binding modes over the simulations. Binding modes are assigned according to the electrostatic interactions observed in panels (<b>b</b>–<b>g</b>). See the text for details.</p>
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<p>CoA’s binding with GPAT1. (<b>a</b>) The centroid of the major cluster found in GPAT1’s MD simulations using the PDB structure (8E50). Positively charged residues within 3 Å of CoA’s phosphate groups are colored in yellow. Residues within 3 Å of CoA’s acyl group are colored in white. The transparent volume depicts all protein residues within 3 Å of CoA, and the color represents the type of residue. Positively charged, negatively charged, hydrophobic and polar residues are colored in blue, red, white and cyan, respectively. The size of the cluster is given as the percentage of the total trajectory length. (<b>b</b>) The centroid of the major cluster found in GPAT1’s AF+ligands simulations. (<b>c</b>) The contact frequency of residues from the non-polar pocket with CoA. These residues appear within 3 Å of CoA throughout almost the entire simulation, except for G190 and F244. (<b>d</b>) The contact frequencies of selected residues with CoA. Selected are those that interact with CoA’s phosphate groups and the catalytic H230. The MD simulations find similar contact frequencies with CoA for both systems (8E50 and AF+ligands), except for K288 and R461. CoA is also frequently in contact with the catalytic H230.</p>
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<p>CoA’s binding with GPAT4. (<b>a</b>–<b>f</b>) Centroids of the first six clusters found in GPAT4’s MD simulations, respectively. As in <a href="#ijms-25-03729-f006" class="html-fig">Figure 6</a>, positively charged residues within 3 Å of CoA’s phosphate groups are colored in yellow, residues within 3 Å of CoA’s acyl group are colored in white and the transparent volume depicts all protein residues within 3 Å of CoA. The color of the volume reflects the properties of the residues: positively charged (blue), negatively charged (red), polar (cyan) and hydrophobic (white). The associated cluster size is listed as the percentage of the total trajectory length. (<b>g</b>) The contact frequency of the residues within 3 Å of the acyl group. Depicted are only those with a frequency larger than 0.5. (<b>h</b>) The contact frequencies of selected residues with CoA. Selected are residues that can interact with CoA’s phosphate groups and the catalytic H248.</p>
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15 pages, 6566 KiB  
Article
Two-Stage Method for Clothing Feature Detection
by Xinwei Lyu, Xinjia Li, Yuexin Zhang and Wenlian Lu
Big Data Cogn. Comput. 2024, 8(4), 35; https://doi.org/10.3390/bdcc8040035 - 26 Mar 2024
Viewed by 1688
Abstract
The rapid expansion of e-commerce, particularly in the clothing sector, has led to a significant demand for an effective clothing industry. This study presents a novel two-stage image recognition method. Our approach distinctively combines human keypoint detection, object detection, and classification methods into [...] Read more.
The rapid expansion of e-commerce, particularly in the clothing sector, has led to a significant demand for an effective clothing industry. This study presents a novel two-stage image recognition method. Our approach distinctively combines human keypoint detection, object detection, and classification methods into a two-stage structure. Initially, we utilize open-source libraries, namely OpenPose and Dlib, for accurate human keypoint detection, followed by a custom cropping logic for extracting body part boxes. In the second stage, we employ a blend of Harris Corner, Canny Edge, and skin pixel detection integrated with VGG16 and support vector machine (SVM) models. This configuration allows the bounding boxes to identify ten unique attributes, encompassing facial features and detailed aspects of clothing. Conclusively, the experiment yielded an overall recognition accuracy of 81.4% for tops and 85.72% for bottoms, highlighting the efficacy of the applied methodologies in garment categorization. Full article
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<p>(<b>a</b>) Examples of top material, including cotton, denim, fur, lace, leather, and tweed; (<b>b</b>) examples of top pattern, including floral, plaid, graphic, solid, spotted, and stripe.</p>
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<p>The structure of the facial feature model.</p>
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<p>The structure of the clothing feature model.</p>
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<p>The process for skin pixel recognition.</p>
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<p>(<b>a</b>) Original image; (<b>b</b>) augmented image.</p>
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<p>(<b>a</b>) Eighteen keypoints distribution map of OpenPose; (<b>b</b>) connecting keypoints by direction to obtaining pose map.</p>
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<p>(<b>a</b>) Dlib facial detection box; (<b>b</b>) a diagram of 68 keypoints generated by Dlib.</p>
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<p>(<b>a</b>) Original image; (<b>b</b>) an example image including body part boxes.</p>
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<p>(<b>a</b>) Age confusion matrix heatmap; (<b>b</b>) gender confusion matrix heatmap.</p>
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<p>(<b>a</b>) Top material confusion matrix heatmap; (<b>b</b>) top pattern confusion matrix heatmap.</p>
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<p>Bottom type confusion matrix heatmap.</p>
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<p>The final result of an example displayed.</p>
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24 pages, 4323 KiB  
Review
Recent Advances in Self-Powered Electronic Skin Based on Triboelectric Nanogenerators
by Qingyang Feng, Yuzhang Wen, Fengxin Sun, Zhenning Xie, Mengqi Zhang, Yunlu Wang, Dongsheng Liu, Zihang Cheng, Yupeng Mao and Chongle Zhao
Energies 2024, 17(3), 638; https://doi.org/10.3390/en17030638 - 29 Jan 2024
Cited by 6 | Viewed by 1701
Abstract
Human skin, the body’s largest organ, plays a crucial role in perceiving mechanical stimulation and facilitating interaction with the external environment. Leveraging the unique attributes of human skin, electronic skin technology aimed at replicating and surpassing the capabilities of natural skin holds significant [...] Read more.
Human skin, the body’s largest organ, plays a crucial role in perceiving mechanical stimulation and facilitating interaction with the external environment. Leveraging the unique attributes of human skin, electronic skin technology aimed at replicating and surpassing the capabilities of natural skin holds significant promise across various domains, including medical care, motion tracking, and intelligent robotics. In recent research, triboelectric nanogenerators have emerged as a compelling solution for addressing the energy challenge in electronic skins. Triboelectric nanogenerators harness the combination of the triboelectric effect and electrostatic induction to efficiently convert mechanical energy into electrical power, serving as self-powered sensors for electronic skins, which possess the advantages of self-powered operation, cost-effectiveness, and compatibility with a wide range of materials. This review provides an introduction to the working principles and the four operational modes of triboelectric nanogenerators, highlighting the functional features of electronic skins, such as stretchability, self-healing, and degradability. The primary focus is on the current applications of self-powered electronic skins based on triboelectric nanogenerators in medical care, motion tracking, and machine tactile recognition. This review concludes by discussing the anticipated challenges in the future development of self-powered electronic skins based on triboelectric nanogenerators. This review holds practical significance for advancing the practical use of self-powered electronic skins based on triboelectric nanogenerators and offers valuable guidance for individuals interested in pursuing scientific and healthy endeavors. Full article
(This article belongs to the Section D1: Advanced Energy Materials)
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<p>Characteristics of self-powered electronic skin based on a triboelectric nanogenerator and its application in various fields.</p>
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<p>The summary of the basic working modes of a triboelectric nanogenerator. (<b>I</b>) Contact-separate mode. (<b>II</b>) Lateral-sliding mode. (<b>III</b>) Single-electrode mode. (<b>IV</b>) Freestanding mode.</p>
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<p>The flexibility and stretchability of e-skin. (<b>a</b>) A flexible and ultra-stretched TENG inspired by the skin of the electric eel. Reprinted with permission from ref. [<a href="#B74-energies-17-00638" class="html-bibr">74</a>], Copyright 2016, Wiley. (<b>b</b>) A flexible and stretchable single-electrode TENG using aloe vera gel (AVG) @NaCl as a liquid electrode. Reprinted with permission from ref. [<a href="#B75-energies-17-00638" class="html-bibr">75</a>], Copyright 2020, Elsevier. (<b>c</b>) A flexible TENG with a stretchability of 800% is used for energy harvesting and tactile perception. Reprinted with permission from ref. [<a href="#B63-energies-17-00638" class="html-bibr">63</a>], Copyright 2021, MDPI. (<b>d</b>) A flexible stretchable Kirigami-structured liquid metal paper for multifunctional e-skin. Reprinted with permission from ref. [<a href="#B49-energies-17-00638" class="html-bibr">49</a>], Copyright 2022, American Chemical Society. (<b>e</b>) A flexible and self-powered e-skin based on a super-stretchable triboelectric nanogenerator (STENG). Reprinted with permission from ref. [<a href="#B76-energies-17-00638" class="html-bibr">76</a>], Copyright 2020, Elsevier. (<b>f</b>) A biologically inspired flexible stretchable TENG e-skin with up to 600% strain. Reprinted with permission from ref. [<a href="#B59-energies-17-00638" class="html-bibr">59</a>], Copyright 2017, Elsevier.</p>
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<p>The self-healing and degradability of e-skin. (<b>a</b>) A single-electrode TENG e-skin that can achieve self-healing within 2.5 min at room temperature. Reprinted with permission from ref. [<a href="#B63-energies-17-00638" class="html-bibr">63</a>], Copyright 2021, MDPI. (<b>b</b>) A healing ionic liquid elastic matrix TENG e-skin. Reprinted with permission from ref. [<a href="#B79-energies-17-00638" class="html-bibr">79</a>], Copyright 2022, Wiley. (<b>c</b>) The use of an MPP-water gel as a TENG electrode can realize self-healing after cutting. Reprinted with permission from ref. [<a href="#B62-energies-17-00638" class="html-bibr">62</a>], Copyright 2022, Elsevier. (<b>d</b>) A self-healable TENG based on double-cross-linked PDMS for the e-skin. Reprinted with permission from ref. [<a href="#B80-energies-17-00638" class="html-bibr">80</a>], Copyright 2022, Elsevier. (<b>e</b>) A biodegradable self-powered e-skin based on an all-nanofiber TENG. Reprinted with permission from ref. [<a href="#B64-energies-17-00638" class="html-bibr">64</a>], Copyright 2020, Amer Assoc Advancement Science. (<b>f</b>) A biodegradable TENG based on chitosan. Reprinted with permission from ref. [<a href="#B58-energies-17-00638" class="html-bibr">58</a>], Copyright 2023, American Chemical Society. (<b>g</b>) A degradable self-powered e-skin integrated with a single-electrode TENG. Reprinted with permission from ref. [<a href="#B83-energies-17-00638" class="html-bibr">83</a>], Copyright 2022, Wiley.</p>
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<p>Permeability and antibacterial properties of e-skin. (<b>a</b>) A breathable e-skin with a micro/nano-layered porous structure. Reprinted with permission from ref. [<a href="#B64-energies-17-00638" class="html-bibr">64</a>], Copyright 2020, Amer Assoc Advancement Science. (<b>b</b>) A breathable e-skin with a nanofiber structure inspired by spider webs and ant tentacles. Reprinted with permission from ref. [<a href="#B86-energies-17-00638" class="html-bibr">86</a>], Copyright 2021, Wiley. (<b>c</b>) A novel breathable e-skin made of feather and a PVDF polymer composite material. Reprinted with permission from ref. [<a href="#B87-energies-17-00638" class="html-bibr">87</a>], Copyright 2023, American Chemical Society. (<b>d</b>) An antibacterial e-skin inspired by the microscopic structure of rose petals. Reprinted with permission from ref. [<a href="#B90-energies-17-00638" class="html-bibr">90</a>], Copyright 2021, Elsevier. (<b>e</b>) An antibacterial TENG e-skin fabricated by sandwiching Ag NWs between TPU and PVA/CS. Photographs of the inhibition zone of (<b>i</b>) TPU, (<b>ii</b>) PVA/CS, and (<b>iii</b>) E-skin before and after incubating for 24 h. Reprinted with permission from ref. [<a href="#B91-energies-17-00638" class="html-bibr">91</a>], Copyright 2021, American Chemical Society. (<b>f</b>) An antibacterial electronic skin based on a double-gradient poly (ionic liquid) nanofiber membrane. Reprinted with permission from ref. [<a href="#B51-energies-17-00638" class="html-bibr">51</a>], Copyright 2021, Wiley.</p>
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<p>Application of TENG e-skin in healthcare. (<b>a</b>) A triboelectric e-skin that can monitor physiological signals. Reprinted with permission from ref. [<a href="#B94-energies-17-00638" class="html-bibr">94</a>], Copyright 2020, Elsevier. (<b>b</b>) A self-powered temperature-sensitive electronic skin based on the triboelectric effect of PDMS/PANI nanostructures. Reprinted with permission from ref. [<a href="#B52-energies-17-00638" class="html-bibr">52</a>], Copyright 2019, Elsevier. (<b>c</b>) A self-powered e-skin based on TENG for the real-time monitoring of respiration and sleep. Reprinted with permission from ref. [<a href="#B95-energies-17-00638" class="html-bibr">95</a>], Copyright 2021, Wiley. (<b>d</b>) A self-powered invisible electronic tattoo sticker that detects sound. Reprinted with permission from ref. [<a href="#B96-energies-17-00638" class="html-bibr">96</a>], Copyright 2021, Wiley. (<b>e</b>) A self-powered and photothermal e-skin patches for accelerating wound healing. Reprinted with permission from ref. [<a href="#B97-energies-17-00638" class="html-bibr">97</a>], Copyright 2022, Elsevier. (<b>f</b>) A liquid single-electrode TENG can monitor tiny facial expressions. Reprinted with permission from ref. [<a href="#B75-energies-17-00638" class="html-bibr">75</a>], Copyright 2020, Elsevier. (<b>g</b>) A self-powered artificial synapse was developed based on TENG. Reprinted with permission from ref. [<a href="#B53-energies-17-00638" class="html-bibr">53</a>], Copyright 2019, Elsevier. (<b>h</b>) A hybrid e-skin combining TENG and humidity sensors detects fall behavior. Reprinted with permission from ref. [<a href="#B98-energies-17-00638" class="html-bibr">98</a>], Copyright 2022, Elsevier.</p>
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<p>Application of TENG e-skin in exercise monitoring. (<b>a</b>) An e-skin that allows for real-time perspiration analysis and movement status monitoring. Reprinted with permission from ref. [<a href="#B101-energies-17-00638" class="html-bibr">101</a>], Copyright 2018, Royal Society of Chemistry. (<b>b</b>) E-skin for real-time perspiration analysis under motion-state monitoring. Reprinted with permission from ref. [<a href="#B102-energies-17-00638" class="html-bibr">102</a>], Copyright 2019, IOP Publishing LTD. (<b>c</b>) An e-skin that can detect finger joint movement based on fiber fabric. Reprinted with permission from ref. [<a href="#B103-energies-17-00638" class="html-bibr">103</a>], Copyright 2017, Royal Society of Chemistry. (<b>d</b>) An e-skin that can monitor different walking states. Reprinted with permission from ref. [<a href="#B56-energies-17-00638" class="html-bibr">56</a>], Copyright 2022, Elsevier. (<b>e</b>) An e-skin based on TENG that can monitor arm swing. Reprinted with permission from ref. [<a href="#B104-energies-17-00638" class="html-bibr">104</a>], Copyright 2022, Amer Assoc Advancement Science. (<b>f</b>) A self-powered e-skin for volleyball receiving statistics and analysis. Reprinted with permission from ref. [<a href="#B91-energies-17-00638" class="html-bibr">91</a>], Copyright 2021, American Chemical Society. (<b>g</b>) A TENG e-skin for human motion monitoring. Reprinted with permission from ref. [<a href="#B105-energies-17-00638" class="html-bibr">105</a>], Copyright 2022, MDPI.</p>
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<p>Application of TENG electronic skin in robot recognition technology. (<b>a</b>) A multimodal e-skin for robotic material and texture recognition. Reprinted with permission from ref. [<a href="#B108-energies-17-00638" class="html-bibr">108</a>], Copyright 2021, Wiley. (<b>b</b>) A TENG e-skin for robot intelligent perception and interaction technology. Reprinted with permission from ref. [<a href="#B110-energies-17-00638" class="html-bibr">110</a>], Copyright 2021, Wiley. (<b>c</b>) A multi-dimensional force sensor electronic skin for robot recognition technology. Reprinted with permission from ref. [<a href="#B111-energies-17-00638" class="html-bibr">111</a>], Copyright 2022, American Chemical Society. (<b>d</b>) A kind of fine texture recognition for robot TENG e-skin. Reprinted with permission from ref. [<a href="#B109-energies-17-00638" class="html-bibr">109</a>], Copyright 2021, Elsevier. (<b>e</b>) An e-skin that can be used for robot temperature–pressure-sensing functions. Reprinted with permission from ref. [<a href="#B112-energies-17-00638" class="html-bibr">112</a>], Copyright 2022, Elsevier. (<b>f</b>) A dual-mode sensor array for multifunctional robot e-skin. Reprinted with permission from ref. [<a href="#B113-energies-17-00638" class="html-bibr">113</a>], Copyright 2019, Elsevier.</p>
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<p>Perspective of self-powered electric skin based on TENG.</p>
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21 pages, 3577 KiB  
Article
In Vitro Pre-Clinical Evaluation of a Gonococcal Trivalent Candidate Vaccine Identified by Transcriptomics
by Shea K. Roe, Brian Felter, Bo Zheng, Sanjay Ram, Lee M. Wetzler, Eric Garges, Tianmou Zhu, Caroline A. Genco and Paola Massari
Vaccines 2023, 11(12), 1846; https://doi.org/10.3390/vaccines11121846 - 13 Dec 2023
Viewed by 2008
Abstract
Gonorrhea, a sexually transmitted disease caused by Neisseria gonorrhoeae, poses a significant global public health threat. Infection in women can be asymptomatic and may result in severe reproductive complications. Escalating antibiotic resistance underscores the need for an effective vaccine. Approaches being explored [...] Read more.
Gonorrhea, a sexually transmitted disease caused by Neisseria gonorrhoeae, poses a significant global public health threat. Infection in women can be asymptomatic and may result in severe reproductive complications. Escalating antibiotic resistance underscores the need for an effective vaccine. Approaches being explored include subunit vaccines and outer membrane vesicles (OMVs), but an ideal candidate remains elusive. Meningococcal OMV-based vaccines have been associated with reduced rates of gonorrhea in retrospective epidemiologic studies, and with accelerated gonococcal clearance in mouse vaginal colonization models. Cross-protection is attributed to shared antigens and possibly cross-reactive, bactericidal antibodies. Using a Candidate Antigen Selection Strategy (CASS) based on the gonococcal transcriptome during human mucosal infection, we identified new potential vaccine targets that, when used to immunize mice, induced the production of antibodies with bactericidal activity against N. gonorrhoeae strains. The current study determined antigen recognition by human sera from N. gonorrhoeae-infected subjects, evaluated their potential as a multi-antigen (combination) vaccine in mice and examined the impact of different adjuvants (Alum or Alum+MPLA) on functional antibody responses to N. gonorrhoeae. Our results indicated that a stronger Th1 immune response component induced by Alum+MPLA led to antibodies with improved bactericidal activity. In conclusion, a combination of CASS-derived antigens may be promising for developing effective gonococcal vaccines. Full article
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Figure 1
<p>IgG against NGO0690, NGO0948 and NGO1701 in human sera from <span class="html-italic">N. gonorrhoeae</span>-infected subjects. Total IgG antibody ELISA of banked de-identified sera from: (<b>A</b>) women with uncomplicated acute gonococcal infection (collected 10–30 days following diagnosis) (<span class="html-italic">n</span> = 25); (<b>B</b>) women convalescing from uncomplicated infection (collected 180–240 days following diagnosis) (<span class="html-italic">n</span> = 25); (<b>C</b>) men with uncomplicated acute gonococcal infection as above (<span class="html-italic">n</span> = 25); and (<b>D</b>) men convalescing from uncomplicated infection as above (<span class="html-italic">n</span> = 25) against purified recombinant NGO0690 (dotted bars), NGO0948 (dashed bars), NGO1701 (striped bars) and <span class="html-italic">N. gonorrhoeae</span> F62 (black bars). Sera (1:100 dilution) were tested in triplicate or quadruplicate, and IgG levels are expressed as the mean of the IgG O.D.<sub>405</sub> minus the blank ± SD for each set of sera against each antigen. *, **, ***, ****—<span class="html-italic">p</span> value is significant according to two-way ANOVA with Tukey’s multiple comparisons test. (<b>E</b>) Banked de-identified sera from women with disseminated gonococcal infection (DGI) (<span class="html-italic">n</span> = 7). Individual data points are shown by different symbols. *, ***, ****—<span class="html-italic">p</span> value is significant according to two-way ANOVA with Tukey’s multiple comparisons test. (<b>F</b>) Commercially available pooled whole normal human serum (NHS) as above. **, ***, ****—<span class="html-italic">p</span> value is significant according to one-way ANOVA with Tukey’s multiple comparisons test.</p>
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<p>Mouse serum IgG antibodies against purified antigens. Total IgG (μg/mL ± SD) in sera from mice immunized with NGO0690+NGO0948+NGO1701 and Alum or Alum+MPLA as an adjuvant measured by ELISA against (<b>A</b>) NGO0690 (dotted bars), (<b>B</b>) NGO0948 (dashed bars) and (<b>C</b>) NGO1701 (striped bars). Preimmune sera, white bars; sera from mice immunized with adjuvant only, gray bars. Sera were tested in triplicate or quadruplicate. ***, ****—<span class="html-italic">p</span> value is significant according to one-way ANOVA with Tukey’s multiple comparisons test. Note the different scale in (<b>A</b>) and (<b>C</b>) vs. (<b>B</b>).</p>
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<p>Mouse vaginal lavage IgG antibodies against purified antigens. Total IgG (μg/mL ± SD) in vaginal lavages from mice immunized with NGO0690+NGO0948+NGO1701 and Alum or Alum+MPLA as an adjuvant measured by ELISA against (<b>A</b>) NGO0690 (dotted bars) and (<b>B</b>) NGO1701 (striped bars). Lavages from mice immunized with adjuvant only, gray bars. Lavages were tested in quadruplicate. *, ***, ****—<span class="html-italic">p</span> value is significant according to one-way ANOVA with Tukey’s multiple comparisons test.</p>
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<p>Mouse serum IgG antibodies against whole <span class="html-italic">N. gonorrhoeae</span>. Total IgG (μg/mL ± SD) in sera from mice immunized with NGO0690+NGO0948+NGO1701 and Alum or Alum+MPLA, measured by whole-cell ELISA against (<b>A</b>) <span class="html-italic">N. gonorrhoeae</span> F62 (black bars) and (<b>B</b>) <span class="html-italic">N. gonorrhoeae</span> MS11 (black bars). Preimmune sera, white bars; sera from mice immunized with adjuvant only, gray bars. (<b>C</b>,<b>D</b>) Total IgG in vaginal lavages from mice immunized with NGO0690+NGO0948+NGO1701 and Alum or Alum+MPLA, measured as above. Lavages from mice immunized with adjuvant only, gray bars. Sera and lavages were tested in triplicate or quadruplicate. *, **, ****—<span class="html-italic">p</span> value is significant according to one-way ANOVA with Tukey’s multiple comparisons test.</p>
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<p>Mouse serum IgG antibody subclasses against whole <span class="html-italic">N. gonorrhoeae</span>. IgG1, IgG2a and IgG3 (μg/mL ± SD) measured by whole-cell ELISA in sera from mice immunized with NGO0690+NGO0948+NGO1701 with Alum (<b>A</b>,<b>B</b>) or with Alum+MPLA (<b>C</b>,<b>D</b>) as adjuvants against (<b>A</b>,<b>C</b>) <span class="html-italic">N. gonorrhoeae</span> F62 (black bars) and (<b>B</b>,<b>D</b>) <span class="html-italic">N. gonorrhoeae</span> MS11 (black bars). Preimmune sera, white bars; adjuvant-only sera, gray bars. Sera were tested in triplicate or quadruplicate. *, **, ***, ****—<span class="html-italic">p</span> value is significant according to one-way ANOVA with Tukey’s multiple comparisons test.</p>
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<p>Mouse serum cytokine profile. Th2 cytokines (<b>A</b>) IL-10 and (<b>B</b>) IL-4, and Th1 cytokines (<b>C</b>) IFN-γ and (<b>D</b>) IL-12p70 measured by ELISA. Adjuvant-only sera (gray bars), NGO0690+NGO0948+NGO1701 and Alum sera and NGO0690+NGO0948+NGO1701 and Alum+MPLA (black bars) were tested in quadruplicate and cytokine levels are expressed in pg/mL ± SD. *, ***, ****—<span class="html-italic">p</span> value is significant according to one-way ANOVA with Tukey’s multiple comparisons test. (<b>E</b>) IL-12p70/IL-10 ratio and IFN-γ/IL-4 ratio. * <span class="html-italic">p</span> &lt; 0.05 according to Mann–Whitney test. (<b>F</b>,<b>G</b>) IL-6 and TNF-α measured as above. ***—<span class="html-italic">p</span> value is significant according to one-way ANOVA with Tukey’s multiple comparisons test.</p>
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<p>Mouse serum IgM antibodies against whole <span class="html-italic">N. gonorrhoeae</span>. IgM (μg/mL ± SD) in sera from mice immunized with NGO0690+NGO0948+NGO1701 and Alum or Alum+MPLA, measured by whole-cell ELISA against (<b>A</b>) <span class="html-italic">N. gonorrhoeae</span> F62 (black bars) and (<b>B</b>) <span class="html-italic">N. gonorrhoeae</span> MS11 (black bars). Preimmune sera, white bars; adjuvant-only sera, gray bars. Sera were tested in triplicate or quadruplicate. *, **, ****—<span class="html-italic">p</span> value is significant according to one-way ANOVA with Tukey’s multiple comparisons test.</p>
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<p>Serum IgM and IgG antibody avidity against <span class="html-italic">N. gonorrhoeae</span> F62. (<b>A</b>) IgM (O.D.<sub>405</sub> minus the blank ± SD) determined with a modified ELISA in the presence (open symbols) or absence (closed symbols) of 8M urea treatment. NGO0690+NGO0948+NGO1701 and Alum sera (triangles) or Alum-alone sera (circles) and (<b>B</b>) NGO0690+NGO0948+NGO1701 and Alum+MPLA sera (triangles) or Alum+MPLA-alone sera (circles). (<b>C</b>) IgG antibody avidity as above. Alum-alone sera (circles) and NGO0690+NGO0949+NGO1701 with Alum sera (squares) and (<b>D</b>) Alum+MPLA-alone sera (circles) and NGO0690+NGO0949+NGO1701 with Alum+MPLA sera (squares). Sera were tested in triplicate. *, ***, ****—<span class="html-italic">p</span> value is significant according to two-way ANOVA with Tukey’s multiple comparisons test.</p>
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<p>Serum bactericidal activity (SBA). <span class="html-italic">N. gonorrhoeae</span> F62 survival (% CFU at T30/T0 ± SD). IgM-depleted sera from mice immunized with Alum alone (gray bar) and NGO0690+NGO0948+NGO1701 with Alum (white bars). Serum dilutions are indicated on the <span class="html-italic">X</span>-axis. <sup>#, ##, ####</sup>—<span class="html-italic">p</span> &lt; 0.05, 0.003 and 0.0001 according to one-way ANOVA with Dunnett’s multiple comparison test vs. the Alum-only sera. *, **, ***, ****—<span class="html-italic">p</span> = 0.04 to &lt;0.0001 according to one-way ANOVA with Tukey’s multiple comparisons test among sera dilutions.</p>
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<p>Serum bactericidal activity (SBA) in the presence of 2% BSA. <span class="html-italic">N. gonorrhoeae</span> F62 survival (% CFU T30/T0 ± SD). Sera from mice immunized with (<b>A</b>) Alum alone (gray bar) or with NGO0690+NGO0948+NGO1701 and Alum (white bars), and (<b>B</b>) Alum+MPLA-alone (gray bar) or NGO0690+NGO0948+NGO1701 and Alum+MPLA (white bars). Serum dilutions are indicated on the <span class="html-italic">X</span>-axis. *, **, ***, ****—<span class="html-italic">p</span> = 0.04 to &lt;0.0001 according to one-way ANOVA with Tukey’s multiple comparisons test among sera dilutions. <sup>##</sup>, <sup>####</sup>—<span class="html-italic">p</span> = 0.002 and &lt;0.0001 according to one-way ANOVA with Tukey’s multiple comparisons test vs. adjuvant alone.</p>
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<p>Images of the predicted structure (side view) of (<b>A</b>) NGO0690, (<b>B</b>) NGO0948 and (<b>C</b>) NGO1701, modeled with Alpha Fold [<a href="#B61-vaccines-11-01846" class="html-bibr">61</a>], based on the protein sequence and colored by confidence (dark blue, highest; orange/yellow, lowest). (<b>b</b>) NGO0948, front view. (<b>A<sub>I</sub></b>–<b>C<sub>I</sub></b>) Surface model images of NGO0690, NGO0948 and NGO1701 (light gray) rendered with PyMol [<a href="#B65-vaccines-11-01846" class="html-bibr">65</a>], showing the predicted linear B cell epitopes (LE) (ElliPro and Bepipred [<a href="#B63-vaccines-11-01846" class="html-bibr">63</a>,<a href="#B64-vaccines-11-01846" class="html-bibr">64</a>]) in different colors. LE in regions with low structure model confidence as in (<b>A</b>–<b>C</b>) is shown by transparent colors, and LE in regions with high confidence in solid colors. (<b>A<sub>II</sub></b>–<b>C<sub>II</sub></b>) Predicted conformational epitopes (CE) obtained and rendered as above. CE in regions of low confidence are shown in transparent dark gray, and CE in regions with high confidence in solid dark gray. In (<b>B<sub>II</sub></b>), CE6 and CE7 are at the back of the image, indicated by an arrow, and are not visible.</p>
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20 pages, 11558 KiB  
Article
Reconstructing the Biogeographic History of the Genus Aurelia Lamarck, 1816 (Cnidaria, Scyphozoa), and Reassessing the Nonindigenous Status of A. solida and A. coerulea in the Mediterranean Sea
by Alfredo Fernández-Alías, Concepción Marcos and Angel Pérez-Ruzafa
Diversity 2023, 15(12), 1181; https://doi.org/10.3390/d15121181 - 29 Nov 2023
Cited by 1 | Viewed by 1677
Abstract
The genus Aurelia is one of the most extensively studied within the class Scyphozoa. However, much of the research was historically attributed to the species Aurelia aurita (Linnaeus, 1758) before the recognition of its taxonomic complexity. Initially considered cosmopolitan and globally distributed, recent [...] Read more.
The genus Aurelia is one of the most extensively studied within the class Scyphozoa. However, much of the research was historically attributed to the species Aurelia aurita (Linnaeus, 1758) before the recognition of its taxonomic complexity. Initially considered cosmopolitan and globally distributed, recent phylogenetic analysis has challenged this assumption. Consequently, the current distribution of species within the genus Aurelia and the processes that led to this distribution remain largely unexplored. After genetically confirming that the species traditionally present in the Mar Menor coastal lagoon in the southwestern Mediterranean corresponds to A. solida, we compiled data on the locations where moon jellyfish species have been genetically identified and mapped these coordinates to the geological period when the genus Aurelia diverged from other scyphozoan genera. We propose two hypotheses to explain the disjunct distribution of certain species. The first one assumes recent human-mediated introductions, while the second posits an absence of introductions. Both hypotheses, supported by fossil and historical records, suggest a Paleo-Tethys origin of the genus Aurelia. Migration from this area explains most of the genus’s current distribution without human intervention, being the Mediterranean Sea, where A. solida should be considered autochthonous, part of their natural distribution range. Full article
(This article belongs to the Special Issue Diversity, Phylogeny and Evolutionary History of Cnidaria)
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<p>Mar Menor location and <span class="html-italic">Aurelia</span> sampling locations.</p>
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<p>Cladogram of the <span class="html-italic">Aurelia</span> genus based on the concatenated genetic analysis (COI + 28S + ITS5.8) of Lawley et al. [<a href="#B1-diversity-15-01181" class="html-bibr">1</a>], complemented with Moura et al. [<a href="#B14-diversity-15-01181" class="html-bibr">14</a>].</p>
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<p>Evolutionary history, as inferred by maximum likelihood, of the genus <span class="html-italic">Aurelia</span> in the Mediterranean Sea. (<b>a</b>) Phylogenetic tree for the 28S rDNA. (<b>b</b>) Phylogenetic tree for the COI genetic marker. Numbers above the tree branches indicate bootstrap support for each branch. Clades were collapsed for a support higher than 0.9 at species level to ease visualization. The tree is drawn to scale, with branch length measured in the same units as the evolutionary distances used for their calculation. Non-collapsed ML and BI trees are provided in <a href="#app1-diversity-15-01181" class="html-app">Figure S1 and Figure S2</a>, respectively.</p>
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<p>Present biogeographic distribution of the genus <span class="html-italic">Aurelia</span> based on genetically identified individuals. Dashed areas indicate the main distribution areas of the different lineages. Yellow: boreal; Red: Atlanto-Mediterranean; Green: western Atlantic; Blue: Indo-Pacific.</p>
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<p>Paleogeography of the different lineages of the genus <span class="html-italic">Aurelia</span> explaining the present disjunct distributions through latter possible anthropic introductions.</p>
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<p>Paleogeography of the different lineages of the genus <span class="html-italic">Aurelia</span> explaining the present disjunct distributions without anthropic introductions.</p>
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