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Search Results (1,392)

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Keywords = heat conductance distribution

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19 pages, 7565 KiB  
Article
Improving Mechanical Properties of Low-Quality Pure Aluminum by Minor Reinforcement with Fine B4C Particles and T6 Heat Treatment
by Maxat Abishkenov, Ilgar Tavshanov, Nikita Lutchenko, Nursultan Amanzholov, Daniyar Kalmyrzayev, Zhassulan Ashkeyev, Kayrosh Nogaev, Saltanat Kydyrbayeva and Assylbek Abdirashit
Appl. Sci. 2024, 14(23), 10773; https://doi.org/10.3390/app142310773 - 21 Nov 2024
Viewed by 162
Abstract
Pure aluminum, due to its inherent low strength and softness, is unsuitable for most structural applications. However, unlike many aluminum alloys, pure aluminum exhibits high ductility and is often free from expensive alloying elements. This makes it a promising candidate for minor reinforcement [...] Read more.
Pure aluminum, due to its inherent low strength and softness, is unsuitable for most structural applications. However, unlike many aluminum alloys, pure aluminum exhibits high ductility and is often free from expensive alloying elements. This makes it a promising candidate for minor reinforcement to produce cost-effective composites with an optimal balance of strength and ductility. This study assesses the possibility of improving the mechanical performance of pure aluminum specimens by minor reinforcement (~0.36 wt. %) with fine B4C particles and T6 heat treatment. The composites were obtained using ultrasonic-assisted stir casting and were characterized by assessing their density, microhardness, yield strength (YS), ultimate tensile strength (UTS), and elongation. Light microscopy (LM), scanning electron microscopy (SEM), energy-dispersive spectroscopy (EDS), and X-ray diffraction (XRD) tests were conducted to investigate the presence and distribution of reinforcing particles in the Al matrix. Minor reinforcement of ~0.5–2 μm with B4C particles without/with subsequent T6 heat treatment resulted in an increase in microhardness by 71.45% and 143.37% and UTS by 71.05% and 140.16%, respectively, while the elongation values of the specimens decreased to 51.98% and 42.38%, respectively, compared with the adopted initial matrix Al specimen. Full article
(This article belongs to the Section Materials Science and Engineering)
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<p>Illustration of the stages of the AMCs fabrication process.</p>
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<p>Illustration of the specimen mass measurement process in the MH-300A densitometer for experimental density determination.</p>
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<p>Illustration of Vickers microhardness measurement using the HVT-1000A microhardness tester (Laizhou Laihua Testing Instrument Factory, Laizhou, China).</p>
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<p>Dimensions (in mm) according to ASTM E8 [<a href="#B31-applsci-14-10773" class="html-bibr">31</a>] and appearance of prepared and tested tensile test specimens.</p>
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<p>SEM images and EDS spectra of boron carbide (B<sub>4</sub>C) and potassium hexafluorotitanate (K<sub>2</sub>TiF<sub>6</sub>) particles.</p>
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<p>LM images of the S1, S1-HT, S2, and S2-HT specimens.</p>
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<p>SEM images and EDS spectra of the S1, S1-HT, S2, and S2-HT specimens.</p>
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<p>Combined XRD patterns of the S1, S1-HT, S2, and S2-HT specimens.</p>
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<p>Combined XRD patterns of S2 and S2-HT specimens (fragmented for B<sub>4</sub>C).</p>
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<p>Density and porosity of S1, S1-HT, S2 and S2-HT specimens.</p>
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<p>Measured microhardness values (HV0.1) of the S1, S1-HT, S2, and S2-HT specimens.</p>
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<p>Stress–strain curves (<b>a</b>) and the histogram comparing the values of yield strength (YS), ultimate tensile strength (UTS), and elongation (<b>b</b>) of the S1, S1-HT, S2, and S2-HT specimens.</p>
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16 pages, 12606 KiB  
Article
Monitoring and Modeling Urban Temperature Patterns in the State of Iowa, USA, Utilizing Mobile Sensors and Geospatial Data
by Clemir Abbeg Coproski, Bingqing Liang, James T. Dietrich and John DeGroote
Appl. Sci. 2024, 14(22), 10576; https://doi.org/10.3390/app142210576 - 16 Nov 2024
Viewed by 455
Abstract
Thorough investigations into air temperature variation across urban environments are essential to address concerns about city livability. With limited research on smaller cities, especially in the American Midwest, the goal of this research was to examine the spatial patterns of air temperature across [...] Read more.
Thorough investigations into air temperature variation across urban environments are essential to address concerns about city livability. With limited research on smaller cities, especially in the American Midwest, the goal of this research was to examine the spatial patterns of air temperature across multiple small to medium-sized cities in Iowa, a relatively rural US state. Extensive fieldwork was conducted utilizing manually built mobile temperature sensors to collect air temperature data at a high temporal and spatial resolution in ten Iowa urban areas during the afternoon, evening, and night on days exceeding 32 °C from June to September 2022. Using the random forest machine-learning algorithm and estimated urban morphological variables at varying neighborhood distances derived from 1 m2 aerial imagery and derived products from LiDAR data, we created 24 predicted surface temperature models that demonstrated R2 coefficients ranging from 0.879 to 0.997 with the majority exceeding an R2 of 0.95, all with p-values < 0.001. The normalized vegetation index and 800 m neighbor distance were found to be the most significant in explaining the collected air temperature values. This study expanded upon previous research by examining different sized cities to provide a broader understanding of the impact of urban morphology on air temperature distribution while also demonstrating utility of the random forest algorithm across cities ranging from approximately 10,000 to 200,000 inhabitants. These findings can inform policies addressing urban heat island effects and climate resilience. Full article
(This article belongs to the Special Issue Geospatial Technology: Modern Applications and Their Impact)
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<p>Cities for which temperature data were collected.</p>
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<p>Temperature sensor devices (Adafruit Sensirion SHT40).</p>
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<p>Workflow to derive urban morphometric independent variables and application of the random forest algorithm.</p>
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<p>Measured temperature in Waterloo/Cedar Falls during the afternoon.</p>
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<p>Measured temperature in Waterloo/Cedar Falls during the evening.</p>
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<p>Measured temperature in Waterloo/Cedar Falls during the night.</p>
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<p>Modeled raster surface for Waterloo/Cedar Falls afternoon.</p>
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<p>Modeled raster surface for Waterloo/Cedar Falls evening.</p>
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<p>Modeled raster surface for Waterloo/Cedar Falls night.</p>
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24 pages, 5691 KiB  
Article
Three-Dimensional CFD Analysis of a Hot Water Storage Tank with Various Inlet/Outlet Configurations
by Alina Abdidin, Abzal Seitov, Amankeldy Toleukhanov, Yerzhan Belyayev, Olivier Botella, Abdelhamid Kheiri and Mohammed Khalij
Energies 2024, 17(22), 5716; https://doi.org/10.3390/en17225716 - 15 Nov 2024
Viewed by 285
Abstract
This study presents a comprehensive 3D numerical analysis of thermal stratification, fluid dynamics, and heat transfer efficiency across six hot water storage tank configurations, identified as Tank-1 through Tank-6. The objective is to determine the most effective design for achieving uniform temperature distribution, [...] Read more.
This study presents a comprehensive 3D numerical analysis of thermal stratification, fluid dynamics, and heat transfer efficiency across six hot water storage tank configurations, identified as Tank-1 through Tank-6. The objective is to determine the most effective design for achieving uniform temperature distribution, stable stratification, and efficient heat retention in sensible heat storage systems, with potential for integration with phase change materials (PCMs). Using COMSOL Multiphysics 5.6, simulations were conducted to evaluate key performance indicators, including the Richardson number, capacity ratio, and exergy efficiency. Among the tanks, Tank-1 demonstrated the highest efficiency, with a capacity ratio of 84.6% and an exergy efficiency of 72.5%, while Tank-3, which achieved a capacity ratio of 70.2% and exergy efficiency of 50.5%, was identified as the most practical for real-world applications due to its balanced heat distribution and feasibility for PCM integration. Calculated dimensionless numbers (Reynolds number: 635, Prandtl number: 4.5, and Peclet number: 2858) indicated laminar flow and dominant convective heat transfer across all the configurations. These findings provide valuable insights into the design of efficient thermal storage systems, with Tank-3’s configuration offering a practical balance of thermal performance and operational feasibility. Future work will explore the inclusion of PCM containers within Tank-3, as well as applications for heat pump and solar water heaters, and high-temperature heat storage with various working fluids. Full article
(This article belongs to the Section D: Energy Storage and Application)
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<p>Schematic configurations of the storage tanks with different inlet and outlet configurations.</p>
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<p>Schematic representation of initial and boundary conditions for Tank-1.</p>
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<p>Three-dimensional computational grid for Tank-3 and Tank-6.</p>
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<p>Time evolution of the temperature at the midpoint of Tank-1 on various meshes of increasing size.</p>
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<p>Experimental configuration for validation purposes: (<b>a</b>) 3D diagram; (<b>b</b>) COMSOL results.</p>
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<p>Validation curve showing the progression of dimensionless temperature across the tank’s height.</p>
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<p>Time-dependent temperature distribution of the heat transfer fluid: (<b>a</b>) Tank-1 during the charging mode; (<b>b</b>) Tank-2 during the discharging mode; (<b>c</b>) Tank-3 during the charging mode; (<b>d</b>) Tank-4 during the discharging mode; (<b>e</b>) Tank-5 during the charging mode; (<b>f</b>) Tank-6 during the discharging mode.</p>
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<p>Time-dependent temperature distribution of the heat transfer fluid: (<b>a</b>) Tank-1 during the charging mode; (<b>b</b>) Tank-2 during the discharging mode; (<b>c</b>) Tank-3 during the charging mode; (<b>d</b>) Tank-4 during the discharging mode; (<b>e</b>) Tank-5 during the charging mode; (<b>f</b>) Tank-6 during the discharging mode.</p>
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<p>Time-dependent temperature distribution of the heat transfer fluid: (<b>a</b>) Tank-1 during the charging mode; (<b>b</b>) Tank-2 during the discharging mode; (<b>c</b>) Tank-3 during the charging mode; (<b>d</b>) Tank-4 during the discharging mode; (<b>e</b>) Tank-5 during the charging mode; (<b>f</b>) Tank-6 during the discharging mode.</p>
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<p>Comparison of capacity ratios and exergy efficiencies for the various tanks.</p>
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<p>Comparison of charging and discharging efficiencies for the tanks.</p>
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<p>Time evolution of the Richardson number for the tanks.</p>
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22 pages, 3347 KiB  
Article
Investigating the Reliability of Heating, Ventilation, and Air Conditioning Systems Utilized in Passenger Vehicles
by Sonali K. Kale, Mahendra Shelar, Shashikant Auti, Prachi V. Ingle, Anindita Roy, Chandrakant R. Sonawane and Rajkumar Bhimgonda Patil
Appl. Sci. 2024, 14(22), 10522; https://doi.org/10.3390/app142210522 - 15 Nov 2024
Viewed by 330
Abstract
A Heating, Ventilation, and Air Conditioning (HVAC) system is often utilized in passenger vehicles to enhance the comfort of both the driver and the passengers. The reliability of an HVAC system refers to the probability that a component within the system will fulfil [...] Read more.
A Heating, Ventilation, and Air Conditioning (HVAC) system is often utilized in passenger vehicles to enhance the comfort of both the driver and the passengers. The reliability of an HVAC system refers to the probability that a component within the system will fulfil its intended function during a specified timeframe while operating according to the predefined operational and environmental conditions. Conducting a reliability analysis for the HVAC system of a passenger vehicle is crucial to ensure safety, comfort, cost-effectiveness, and a positive standing. A methodology for analyzing the reliability analysis of a HVAC system using field failure data were developed to identify the critical failure modes, components, and subsystems. A detailed Pareto analysis was applied at subsystem and failure mode levels in order to prioritize them accordingly to their failure frequency. The analysis showed that the A/C evaporator and blower front sides were observed to be the most critical subsystems, contributing to approximately 50% of all failures. Furthermore, the leakages at the joints and vibrations are the primary failure modes of the HVAC system. The Weibull++ software package (version 2021) was used to estimate the best-fit probability distributions for each subsystem and system reliability modelling using a Reliability Block Diagram. The results show that the exponential distribution fits well for several subsystem’s Time-To-Failure (TTF) data and show that the failures were random and due to external reasons. Full article
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<p>Keyword co-occurrence in the HVAC system reliability and fault detection literature.</p>
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<p>Proposed methodology for reliability and failure analysis of an HVAC system.</p>
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<p>Block diagram of an HVAC system.</p>
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<p>Pareto chart for the subsystems of the HVAC system.</p>
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<p>Pareto chart of the A/C evaporator front side.</p>
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<p>Pareto chart of the blower front side.</p>
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<p>Pareto chart of the A/C gas leak at joint.</p>
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<p>Pareto analysis of the A/C compressor.</p>
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<p>Pareto chart of the A/C hose cut/leaking.</p>
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<p>Pareto chart of the HVAC control panel.</p>
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<p>Pareto analysis of A/C condenser front side.</p>
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<p>Pareto chart for all A/C louvers.</p>
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<p>The HVAC subsystem’s reliability versus time plot.</p>
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<p>The HVAC subsystem’s unreliability versus time plot.</p>
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<p>The reliability block diagram for the analyzed HVAC system.</p>
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19 pages, 3072 KiB  
Article
Coordinate-Corrected and Graph-Convolution-Based Hand Pose Estimation Method
by Dang Rong and Feng Gang
Sensors 2024, 24(22), 7289; https://doi.org/10.3390/s24227289 - 14 Nov 2024
Viewed by 264
Abstract
To address the problem of low accuracy in joint point estimation in hand pose estimation methods due to the self-similarity of fingers and easy self-obscuration of hand joints, a hand pose estimation method based on coordinate correction and graph convolution is proposed. First, [...] Read more.
To address the problem of low accuracy in joint point estimation in hand pose estimation methods due to the self-similarity of fingers and easy self-obscuration of hand joints, a hand pose estimation method based on coordinate correction and graph convolution is proposed. First, the standard coordinate encoding is improved by generating an unbiased heat map, and the distribution-aware method is used for decoding coordinates to reduce the error in decoding the coordinate encoding of joints. Then, the complex dependency relationship between the joints and the relationship between pixels and joints of the hand are modeled by using graph convolution, and the feature information of the hand joints is enhanced by determining the relationship between the hand joints. Finally, the skeletal constraint loss function is used to impose constraints on the joints, and a natural and undistorted hand skeleton structure is generated. Training tests are conducted on the public gesture interaction dataset STB, and the experimental results show that the method in this paper can reduce errors in hand joint point detection and improve the estimation accuracy. Full article
(This article belongs to the Section Sensing and Imaging)
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<p>A global network model for hand pose estimation.</p>
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<p>Hourglass network model.</p>
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<p>Residual block module.</p>
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<p>Joint graph reasoning module.</p>
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<p>Skeletal topology of the hand.</p>
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<p>Hand pose estimation visualization results.</p>
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<p>Comparison of the experimental results of different methods.</p>
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18 pages, 13756 KiB  
Article
A Study on the Effect of Cutting Temperature on CFRP Hole Wall Damage in Continuous Drilling Process
by Chong Zhang, Feiyu Chen, Dongxue Song, Jiale Liu, Qingsong Xu, Qunli Zhou and Haoyu Wang
Machines 2024, 12(11), 809; https://doi.org/10.3390/machines12110809 - 14 Nov 2024
Viewed by 247
Abstract
In the assembly process of aerospace parts, drilling is essential for carbon fiber-reinforced materials. However, due to the extreme thermal sensitivity of these composites, continuous drilling often leads to irreparable defects such as hole wall burns and exit delamination caused by concentrated cutting [...] Read more.
In the assembly process of aerospace parts, drilling is essential for carbon fiber-reinforced materials. However, due to the extreme thermal sensitivity of these composites, continuous drilling often leads to irreparable defects such as hole wall burns and exit delamination caused by concentrated cutting heat, resulting in the scrapping of parts. To address this issue, this paper explores the impact of temperature characteristics on drilling quality, providing guidance for optimizing the composite drilling process. A simulation model for single and continuous drilling was established to analyze the temperature distribution on the tool surface during drilling. A drilling temperature measurement system based on thin-film thermocouple technology was developed, enabling real-time online temperature monitoring. Continuous drilling experiments were conducted, analyzing the correlation between maximum drilling temperature and hole quality. Results show that temperatures from −25.75 °C to −9.75 °C and from 182 °C to 200.75 °C cause significant exit damage, while optimal hole quality is achieved between −1.25 °C and 168 °C. Full article
(This article belongs to the Special Issue Composites Machining in Manufacturing)
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<p>Geometric model and stacking diagram of CFRP laminate.</p>
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<p>Tool geometry model.</p>
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<p>Visualization results for temperature field of single drilling model.</p>
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<p>Process of tool temperature field change after single drilling.</p>
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<p>Thrust force output results during single drilling process.</p>
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<p>Progressive delamination damage process during single drilling.</p>
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<p>Temperature field output results for the ninth drilling process.</p>
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<p>Simulation of the variation in maximum temperature and delamination damage with the number of the drilling process.</p>
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<p>Comparison of stratified damage variable with delamination damage in holes 1 and 9.</p>
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<p>The drilling material object and geometric modeling.</p>
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<p>Static calibration data and fitting results.</p>
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<p>Physical image of temperature-measuring tools.</p>
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<p>CFRP drilling temperature testing system.</p>
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<p>The change in outlet layer temperature and damage factor with the number of holes in ordinary dry cutting.</p>
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<p>Morphology of the outlet layer of each hole in ordinary dry cutting.</p>
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<p>The variation in outlet layer temperature and hole wall surface roughness with the number of holes in ordinary dry cutting.</p>
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<p>Changes in outlet layer temperature and damage factor with the number of holes drilled under low-flow cooling conditions.</p>
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<p>Morphology of outlet layers of each hole during low-flow cooling processing.</p>
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<p>The variation in outlet layer temperature and hole wall surface roughness with the number of holes drilled.</p>
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<p>Change in the damage factor and surface roughness of hole wall with the number of holes drilled.</p>
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<p>Change in the damage factor and surface roughness of hole wall with the number of holes drilled.</p>
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<p>Variation in outlet layer temperature and hole wall surface roughness with the number of holes drilled.</p>
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<p>Comparison of damage factors and average surface roughness values obtained with continuous drilling under three different conditions.</p>
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31 pages, 4906 KiB  
Article
The Impact of Residential Building Insulation Standards on Indoor Thermal Environments and Heat-Related Illness Risks During Heatwaves: A Case Study in Korea
by Hee Jung Ham, Sungsu Lee and Ho-Jeong Kim
Sustainability 2024, 16(22), 9831; https://doi.org/10.3390/su16229831 - 11 Nov 2024
Viewed by 534
Abstract
This study investigates the impact of building insulation standards on indoor thermal environments and the risk of heat-related illnesses during heatwaves in South Korea. Indoor temperatures were measured in residential buildings located in Chuncheon and Gwangju during the 2022 heatwave, with outdoor temperature [...] Read more.
This study investigates the impact of building insulation standards on indoor thermal environments and the risk of heat-related illnesses during heatwaves in South Korea. Indoor temperatures were measured in residential buildings located in Chuncheon and Gwangju during the 2022 heatwave, with outdoor temperature data sourced from the Korea Meteorological Administration. Probability distribution fitting was used to estimate the likelihood of indoor temperatures exceeding the critical threshold of 27 °C. Additionally, a linear regression analysis was conducted to examine the relationship between the probability of exceeding the threshold and heat-related illness data from 2017 to 2023 provided by the Korea Disease Control and Prevention Agency. The findings reveal significant variations in indoor thermal conditions during heatwaves, influenced by factors such as building type, year of construction, and climate region, which affect the thermal insulation performance. Buildings with a lower thermal insulation performance were associated with higher indoor temperatures, increasing the likelihood of exceeding the critical threshold and contributing to a higher incidence of heat-related illnesses, particularly in provincial non-metropolitan areas. These results underscore the need for region-specific building insulation standards that address both winter energy efficiency and summer heatwave resilience. Enhancing thermal insulation in vulnerable regions could significantly reduce the risk of heat-related illnesses and improve public health resilience to extreme heat events. Full article
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<p>Flowchart illustrating the study’s assessment of the impact of thermal insulation performance standards on indoor thermal environments during heatwaves (<b>top</b>) and the influence of the standards on indoor thermal exposures and the occurrence of heat-related illnesses during heatwaves (<b>bottom</b>).</p>
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<p>The three climate regions (Central, Southern, and Jeju) and administrative districts (provinces and metropolitan cities) [<a href="#B24-sustainability-16-09831" class="html-bibr">24</a>], with experimental regions marked with circles.</p>
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<p>Locations of residential buildings, weather stations, and representative buildings where experiments were conducted (top: Chuncheon; bottom: Gwangju) (NTS).</p>
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<p>Tzone TempU 03 temperature data logger.</p>
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<p>Cumulative density function of indoor-to-outdoor temperature ratio (thermal transmittance: 0.76 W/m<sup>2</sup>·K).</p>
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<p>Schematics of the methodology for simulating indoor temperatures and the probability of exceeding the threshold temperature during heatwaves.</p>
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<p>Step-by-step process for analyzing correlations between regions.</p>
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<p>Relationship between air conditioning usage and daily average indoor temperature.</p>
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<p>Simulated probability of exceeding the indoor threshold temperature (27 °C) during heatwaves based on building thermal insulation performance.</p>
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<p>Regression analysis between the average probability of exceeding the indoor threshold temperature and the number of indoor heat-related illnesses across regions.</p>
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12 pages, 1556 KiB  
Article
Thermally Conductive Polydimethylsiloxane-Based Composite with Vertically Aligned Hexagonal Boron Nitride
by Haosen Lin, Genghao Xu, Zihao Chen, Luyang Wang, Zhichun Liu and Lei Ma
Polymers 2024, 16(22), 3126; https://doi.org/10.3390/polym16223126 - 8 Nov 2024
Viewed by 492
Abstract
The considerable heat generated in electronic devices, resulting from their high-power consumption and dense component integration, underscores the importance of developing effective thermal interface materials. While composite materials are ideal for this application, the random distribution of filling materials leads to numerous interfaces, [...] Read more.
The considerable heat generated in electronic devices, resulting from their high-power consumption and dense component integration, underscores the importance of developing effective thermal interface materials. While composite materials are ideal for this application, the random distribution of filling materials leads to numerous interfaces, limiting improvements in thermal transfer capabilities. An effective method to improve the thermal conductivity of composites is the alignment of anisotropic fillers, such as hexagonal boron nitride (BN). In this study, the repeat blade coating method was employed to horizontally align BN within a polydimethylsiloxane (PDMS) matrix, followed by flipping and cutting to prepare BN/PDMS composites with vertically aligned BN (V-BP). The V-BP composite with 30 wt.% BN exhibited an enhanced out-of-plane thermal conductivity of up to 1.24 W/mK. Compared to the PDMS, the V-BP composite exhibited outstanding heat dissipation capacities. In addition, its low density and exceptional electrical insulation properties showcase its potential for being used in electronic devices. The impact of coating velocity on the performance of the composites was further studied through computational fluid dynamics simulation. The results showed that increasing the coating velocity enhanced the out-of-plane thermal conductivity of the V-BP composite by approximately 40% compared to those prepared at slower coating velocities. This study provides a promising approach for producing thermal interface materials on a large scale to effectively dissipate the accumulated heat in densely integrated electronic devices. Full article
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Graphical abstract

Graphical abstract
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<p>(<b>a</b>) Schematic illustration of V-BP composite preparation. Optical images of (<b>b</b>) H-BP-30-100 composite, (<b>c</b>) V-BP-30-100 composite, and (<b>d1</b>,<b>d2</b>) bent and recovered V-BP-30-100 composite, respectively.</p>
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<p>(<b>a</b>) Direction of <math display="inline"><semantics> <mrow> <msub> <mi>λ</mi> <mo>⊥</mo> </msub> </mrow> </semantics></math> and <math display="inline"><semantics> <mrow> <msub> <mi>λ</mi> <mo>∥</mo> </msub> </mrow> </semantics></math>. (<b>b</b>) Thermal conductivity of the V-BP composite with different contents of BN prepared at a coating velocity of 100 mm/s. (<b>c</b>) Thermal conductivity of V-BP-30 composites prepared at different coating velocities. (<b>d</b>) Illustration of the performance test for thermal diffusion. (<b>e</b>) IR camera images of the surface of the composites and (<b>f</b>) the temperature variation in the composites while heating. (<b>g</b>) Comparison of the thermal conductivity enhancement of the V-BP-30-100 composite with BN-containing composites reported in References [<a href="#B18-polymers-16-03126" class="html-bibr">18</a>,<a href="#B37-polymers-16-03126" class="html-bibr">37</a>,<a href="#B38-polymers-16-03126" class="html-bibr">38</a>,<a href="#B39-polymers-16-03126" class="html-bibr">39</a>,<a href="#B40-polymers-16-03126" class="html-bibr">40</a>,<a href="#B41-polymers-16-03126" class="html-bibr">41</a>,<a href="#B42-polymers-16-03126" class="html-bibr">42</a>,<a href="#B43-polymers-16-03126" class="html-bibr">43</a>,<a href="#B44-polymers-16-03126" class="html-bibr">44</a>]. (<b>h</b>) Tensile stress–strain curve and (<b>i</b>) fracture toughness of the V-BP composites with a 30 wt.% loading of BN.</p>
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<p>Analysis of the orientation of BN in the composite. (<b>a</b>) The XRD pattern of V-BP-30 composites prepared at different coating velocities and the R-BP-30 composite. (<b>b</b>) Illustration of the ideal distribution of BN in the V-BP composite. The SEM images of (<b>c</b>) the R-BP-30 composite, (<b>d</b>) the V-BP-30-5 composite, (<b>e</b>) the V-BP-30-30 composite, and (<b>f</b>) the V-BP-30-100 composite.</p>
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<p>Impact of coating velocity on the horizontal alignment of BN. (<b>a</b>) Illustration of the rational processes of BN from random to parallel to the shearing direction. (<b>b</b>) Fluid model used in the Ansys CFD 2022 simulation. (<b>c1</b>–<b>e1</b>) The shearing rate, (<b>c2</b>–<b>e2</b>) the expansion rate, and (<b>c3</b>–<b>e3</b>) the absolute value of the ratio of the shearing rate and the expansion rate when the coating velocity is 5, 30, 100 mm/s, respectively.</p>
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<p>Thermal conductivity of V-BP-30 composites prepared at a coating velocity of 100 mm/s and different coating thicknesses.</p>
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18 pages, 7849 KiB  
Article
Evaluation of the Heat Transfer Performance of a Device Utilizing an Asymmetric Pulsating Heat Pipe Structure Based on Global and Local Analysis
by Dong Liu, Jianhong Liu, Kai Yang, Fumin Shang, Chaofan Zheng and Xin Cao
Energies 2024, 17(22), 5588; https://doi.org/10.3390/en17225588 - 8 Nov 2024
Viewed by 432
Abstract
PHPs (pulsating heat pipes) are widely used as an efficient heat transfer element in equipment thermal management and waste heat recovery due to their flexibility. The purpose of this study was to design a heat transfer device that utilizes an asymmetric pulsating heat [...] Read more.
PHPs (pulsating heat pipes) are widely used as an efficient heat transfer element in equipment thermal management and waste heat recovery due to their flexibility. The purpose of this study was to design a heat transfer device that utilizes an asymmetric pulsating heat pipe structure by adjusting the lengths of selected pipes within the entire circulation pipeline. In the experiment, a constant temperature water bath was used as the heat source, with heat dissipated in the condensing section via natural convection. An infrared thermal imager was used to record the temperature of the condensing section, and the local wall temperature distribution was measured in different channels of the condensing section. Based on an in-depth analysis of the wavelet frequency, the following research conclusions are drawn: Firstly, as the heat source temperature increases, the start-up time of the pulsating heat pipe is shortened, the operating state changes from start–stop–start to stable and continuous oscillation, and the oscillation mode changes from high amplitude and low frequency to low amplitude and high frequency. These changes are especially pronounced when the heat source temperature is 80 °C, which is when the thermal resistance reaches its lowest value of 0.0074 K/W, and the equivalent thermal conductivity reaches its highest value of 666.29 W/(m·K). Secondly, the flow and oscillation of the working fluid can be effectively promoted by appropriately shortening the length of the condensing section of the pulsating heat pipes or the heat transfer distance between the evaporation and condensing sections. Third, under a low-temperature heat source, the oscillation frequency of each channel of a pulsating heat pipe is found to be low based on wavelet analysis. However, as the heat source temperature increases, the energy content of the temperature signal of the working fluid in each channel changes from a low- to a high-frequency value, gradually converging to the same characteristic frequency. At this point, the working fluid in the pipes no longer flows randomly in multiple directions but rather in a single direction. Finally, we determined that the maximum oscillation frequency of working fluid in a PHP is around 0.7 HZ when using the water bath heating method. Full article
(This article belongs to the Section J: Thermal Management)
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<p>(<b>a</b>) Internal structure of the device. (<b>b</b>) Exterior structural diagram. (<b>c</b>) Experimental system diagram. (<b>d</b>) Real diagram of the experimental system.</p>
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<p>Average temperature changes in the evaporating and condensing sections for heat source temperatures of (<b>a</b>) 40 °C, (<b>b</b>) 50 °C, (<b>c</b>) 60 °C, (<b>d</b>) 70 °C, and (<b>e</b>) 80 °C.</p>
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<p>Variations in thermal resistance and equivalent thermal conductivity for different heat source temperatures. (<b>a</b>) Thermal resistance. (<b>b</b>) Equivalent thermal conductivity.</p>
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<p>Wall temperature distribution of the condensing sections of the C1, C2, C3, and C4 channels for a heat source temperature of 40 °C.</p>
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<p>Wall temperature distribution of the condensing sections of the C1, C2, C3, and C4 channels for a heat source temperature of 60 °C.</p>
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<p>Wall temperature distribution of the condensing sections of the C1, C2, C3, and C4 channels for a heat source temperature of 60 °C.</p>
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<p>Wall temperature distribution of the condensing sections of the C1, C2, C3, and C4 channels for a heat source temperature of 80 °C.</p>
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<p>Wavelet scale plots of the C1, C2, C3, and C4 channels for a heat source temperature of 40 °C.</p>
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<p>Wavelet scale plots of the C1, C2, C3, and C4 channels for a heat source temperature of 40 °C.</p>
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<p>Wavelet scale plots of the C1, C2, C3, and C4 channels for a heat source temperature of 60 °C.</p>
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<p>Wavelet scale plots of the C1, C2, C3, and C4 channels for a heat source temperature of 80 °C.</p>
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<p>Power mapping.</p>
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<p>Power mapping.</p>
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18 pages, 24816 KiB  
Article
Insights into Adaption and Growth Evolution: Genome–Wide Copy Number Variation Analysis in Chinese Hainan Yellow Cattle Using Whole–Genome Re–Sequencing Data
by Ziqi Zhong, Ziyi Wang, Xinfeng Xie, Deyou Pan, Zhiqing Su, Jinwei Fan, Qian Xiao and Ruiping Sun
Int. J. Mol. Sci. 2024, 25(22), 11919; https://doi.org/10.3390/ijms252211919 - 6 Nov 2024
Viewed by 415
Abstract
Copy number variation (CNV) serves as a crucial source of genomic variation and significantly aids in the mining of genomic information in cattle. This study aims to analyze re–sequencing data from Chinese Hainan yellow cattle, to uncover breed CNV information, and to elucidate [...] Read more.
Copy number variation (CNV) serves as a crucial source of genomic variation and significantly aids in the mining of genomic information in cattle. This study aims to analyze re–sequencing data from Chinese Hainan yellow cattle, to uncover breed CNV information, and to elucidate the resources of population genetic variation. We conducted whole–genome sequencing on 30 Chinese Hainan yellow cattle, thus generating 814.50 Gb of raw data. CNVs were called using CNVnator software, and subsequent filtering with Plink and HandyCNV yielded 197,434 high–quality CNVs and 5852 CNV regions (CNVRs). Notably, the proportion of deleted sequences (81.98%) exceeded that of duplicated sequences (18.02%), with the lengths of CNVs predominantly ranging between 20 and 500 Kb This distribution demonstrated a decrease in CNVR count with increasing fragment length. Furthermore, an analysis of the population genetic structure using CNVR databases from Chinese, Indian, and European commercial cattle breeds revealed differences between Chinese Bos indicus and Indian Bos indicus. Significant differences were also observed between Hainan yellow cattle and European commercial breeds. We conducted gene annotation for both Hainan yellow cattle and European commercial cattle, as well as for Chinese Bos indicus and Indian Bos indicus, identifying 206 genes that are expressed in both Chinese and Indian Bos indicus. These findings may provide valuable references for future research on Bos indicus. Additionally, selection signatures analysis based on Hainan yellow cattle and three European commercial cattle breeds identified putative pathways related to heat tolerance, disease resistance, fat metabolism, environmental adaptation, candidate genes associated with reproduction and the development of sperm and oocytes (CABS1, DLD, FSHR, HSD17B2, KDM2A), environmental adaptation (CNGB3, FAM161A, DIAPH3, EYA4, AAK1, ERBB4, ERC2), oxidative stress anti–inflammatory response (COMMD1, OXR1), disease resistance (CNTN5, HRH4, NAALADL2), and meat quality (EHHADH, RHOD, GFPT1, SULT1B1). This study provides a comprehensive exploration of CNVs at the molecular level in Chinese Hainan yellow cattle, offering theoretical support for future breeding and selection programs aimed at enhancing qualities of this breed. Full article
(This article belongs to the Special Issue Molecular Progression of Genetics in Breeding of Farm Animals)
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<p>CNV variants across chromosomes. This figure presents a statistical analysis of CNV variant types across chromosomes. In the figure, ‘0’ denotes homozygous deletions (loss of 2 copies), ‘1’ represents heterozygous deletions (loss of 1 copy), ‘3’ indicates copy number gain, and ‘4’ denotes amplification (≥2 copies gain). (<b>a</b>) Summary of the total counts of variants for each type by length. (<b>b</b>) Boxplot depicting the statistical analysis of the average lengths of variants for each type.</p>
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<p>CNV fragment statistics and chromosome distribution of CNVR in Hainan yellow cattle. (<b>a</b>) Quantitative analysis of CNV data in Hainan yellow cattle. Red regions indicate deletions, while blue regions represent duplications. (<b>b</b>) The distribution of CNVRs across chromosomes in Hainan yellow cattle. ‘Gain’ denotes regions with duplications, ‘Loss’ signifies regions with deletions, and ‘Mixed’ indicates areas where both duplications and deletions are present simultaneously.</p>
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<p>Genomic CNVR annotation information from the Hainan yellow cattle database. (<b>a</b>) Genomic CNVR annotation information based on the reference genome, where different colors indicating distinct regions. (<b>b</b>) Annotation information of coding regions in Hainan yellow cattle, including frameshift deletions, non–frameshift deletions that do not alter protein coding sequences, and regions of unknown functionality.</p>
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<p>Population genetic structure analysis of Chinese, Indian, and European commercial cattle breeds based on CNVR variations. TB: Tibetan cattle (Tibet, China); CDM: Chaidamu cattle (Qinghai, China); LN: Lingnan cattle (Shanxi, China); NY: Nanyang cattle (Henan, China); GF: Guangfeng cattle (Qinghai, China); HN: Hainan yellow cattle (Qinghai, China); Gir: Gir cattle (Jiangxi, China); Brahman: Brahman cattle (Gujarat, India); AGS: Angus cattle (Aberdeenshire, Scotland, United Kingdom); RAGS: Red Angus cattle (Aberdeenshire, Scotland, United Kingdom); HF: Hereford cattle (Hereford, United Kingdom). (<b>a</b>) Neighbor–joining (NJ) tree constructed based on CNVR variations for Chinese, Indian, and European commercial cattle breeds. (<b>b</b>) Principal component analysis (PCA) plot showing the distribution of Hainan yellow cattle and European commercial cattle breeds along PC1 and PC2 dimensions. The red circle indicates the clustering of Hainan Yellow Cattle, while the blue circle highlights the clustering of European commercial cattle breeds. (<b>c</b>) PCA plot showing the distribution of Chinese and Indian Bos indicus along PC1 and PC2 dimensions. The red circle indicates the clustering of Southern Chinese Bos indicus. (<b>d</b>) Population structure inferred using Bayesian inference method (K = 4–10). Different colors represent distinct subgroups.</p>
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<p>Venn diagram of exonic genes across different cattle populations. (<b>a</b>) Hainan yellow cattle and European commercial cattle breeds. (<b>b</b>) Bos indicus breeds from China and India. GF: Guangfeng cattle; HN: Hainan yellow cattle; Gir: Gir cattle; Brahman: Brahman cattle; AGS: Angus cattle; RAGS: Red Angus cattle, HF: Hereford cattle.</p>
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<p>Manhattan plot of Fixation index (<span class="html-italic">F<sub>ST</sub></span>) for positively selected regions within CNVRs of Hainan yellow cattle, with regions above the red line indicating the top 1% under selection. The different colors represent different chromosomes. The red line represents the critical value.</p>
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<p>Bubble charts depicting enriched gene ontology (GO) terms based on <span class="html-italic">F<sub>ST</sub></span> –filtered candidate genes. (<b>a</b>) Bubble plot of significant GO biological process enrichment analysis based on genes enriched in CNVRs (<span class="html-italic">p</span> &lt; 0.05). (<b>b</b>) Bubble plot of significant GO cellular component enrichment analysis results based on genes enriched in CNVRs (<span class="html-italic">p</span> &lt; 0.05). (<b>c</b>) Bubble plot of significant GO) molecular function enrichment analysis results based on genes enriched in CNVRs (<span class="html-italic">p</span> &lt; 0.05).</p>
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16 pages, 8722 KiB  
Article
Evaluation of the Predictive Capability of CMA Climate Prediction System Model for Summer Surface Heat Source on the Tibetan Plateau
by Xinyu Chen, Minhong Song, Yaqi Wang and Tongwen Wu
Remote Sens. 2024, 16(21), 4118; https://doi.org/10.3390/rs16214118 - 4 Nov 2024
Viewed by 460
Abstract
Surface heat source (SHS) is a crucial factor affecting local weather systems. Particularly SHS on the Tibetan Plateau (TP) significantly influences East Asian atmospheric circulation and global climate. Accurate prediction of summer SHS on the TP is of urgent demand for economic development [...] Read more.
Surface heat source (SHS) is a crucial factor affecting local weather systems. Particularly SHS on the Tibetan Plateau (TP) significantly influences East Asian atmospheric circulation and global climate. Accurate prediction of summer SHS on the TP is of urgent demand for economic development and local climate change. To evaluate the performance of SHS on the TP, the observed SHS data from the eleven sites on the TP verified against CRA40-land (CRA) is evidenced significantly better than ERA5-land (ERA5), another widely used reanalysis. The predictive capability of the CMA Climate Prediction System Model (CMA-CPS) for SHS on the TP was assessed using multiple scoring methods, including the anomaly correlation coefficient and temporal correlation coefficient, among others. Furthermore, relative variability and trend analysis were conducted. Finally, based on these assessments, the causes of the biases were preliminarily discussed. The CMA-CPS demonstrates a reasonable ability to predict the spatial distribution patterns of SHS, sensible heat (SH), and latent heat (LH) on the TP in summer. Specifically, the prediction results of SHS and LH exhibit an “east-high and west-low” distribution, while the distribution of the predicted SH is opposite. Nevertheless, the predicted values are generally lower than CRA, particularly in interannual variations and trends. Among the predictions, LH exhibits the highest temporal correlation coefficients, consistently above 0.6, followed by SHS, while SH predictions are less accurate. The spatial distribution and skill scores indicate that LH on the TP contributes more significantly to SHS than SH in summer. Furthermore, discrepancies in the predictions of surface temperature gradients, ground wind speed, and humidity on the TP may partly explain the biases in SHS and their components. Full article
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<p>Taylor diagrams of SH (<b>a</b>,<b>d</b>), LH (<b>b</b>,<b>e</b>), and SHS (<b>c</b>,<b>f</b>) from different data sources at 11 observation stations on the TP during July–August. Red represents ERA5 data, blue represents CRA data, green represents CMA−CPS data, and the dashed line indicates the root mean square error.</p>
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<p>Spatial distribution of SH on the TP from CMA−CPS (<b>top</b>) and CRA (<b>middle</b>) from 2001 to 2021, and their differences (<b>bottom</b>) (unit: W/m<sup>2</sup>). (<b>a</b>,<b>e</b>,<b>i</b>) Summer, (<b>b</b>,<b>f</b>,<b>j</b>) June, (<b>c</b>,<b>g</b>,<b>k</b>) July, (<b>d</b>,<b>h</b>,<b>l</b>) August. The dotted areas represent regions with a relative variability of 0–10%.</p>
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<p>Spatial distribution of the climatic trend rate of SH on the TP from CMA−CPS (<b>top</b>) and CRA (<b>bottom</b>) from 2001 to 2021 (unit: W/m<sup>2</sup>·(10a)<sup>−1</sup>). (<b>a</b>,<b>e</b>) Summer, (<b>b</b>,<b>f</b>) June, (<b>c</b>,<b>g</b>) July, (<b>d</b>,<b>h</b>) August. The dotted areas indicate regions that passed the 90% significance test.</p>
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<p>Time series of skill scores for SH on the TP predicted by CMA−CPS from 2001 to 2021. (<b>a</b>) <span class="html-italic">MAE</span>, (<b>b</b>) <span class="html-italic">RRMSE</span>, (<b>c</b>) <span class="html-italic">ACC</span>, (<b>d</b>) <span class="html-italic">TCC</span>.</p>
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<p>Spatial distribution of skill scores for SH on the TP predicted by CMA−CPS during different periods from 2001 to 2021. (<b>a</b>–<b>d</b>) <span class="html-italic">MAE</span>, (<b>e</b>–<b>h</b>) <span class="html-italic">RRMSE</span>, (<b>i</b>–<b>l</b>) <span class="html-italic">ACC</span>, (<b>m</b>–<b>p</b>) <span class="html-italic">TCC</span>.</p>
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<p>Spatial distribution of LH on the TP from CMA−CPS (<b>top</b>) and CRA (<b>middle</b>) from 2001 to 2021, and their differences (<b>bottom</b>) (unit: W/m<sup>2</sup>). (<b>a</b>,<b>e</b>,<b>i</b>) Summer, (<b>b</b>,<b>f</b>,<b>j</b>) June, (<b>c</b>,<b>g</b>,<b>k</b>) July, (<b>d</b>,<b>h</b>,<b>l</b>) August. The dotted areas represent regions with a relative variability of 0–10%.</p>
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<p>Spatial distribution of the climatic trend rate of LH on the TP from CMA−CPS (<b>top</b>) and CRA (<b>bottom</b>) from 2001 to 2021 (unit: W/m<sup>2</sup>·(10a)<sup>−1</sup>). (<b>a</b>,<b>e</b>) Summer, (<b>b</b>,<b>f</b>) June, (<b>c</b>,<b>g</b>) July, (<b>d</b>,<b>h</b>) August. The dotted areas indicate regions that passed the 90% significance test.</p>
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<p>Time series of skill scores for LH on the TP predicted by CMA−CPS from 2001 to 2021. (<b>a</b>) <span class="html-italic">MAE</span>, (<b>b</b>) <span class="html-italic">RRMSE</span>, (<b>c</b>) <span class="html-italic">ACC</span>, (<b>d</b>) <span class="html-italic">TCC</span>.</p>
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<p>Spatial distribution of SHS on the TP (unit: W/m<sup>2</sup>) from CMA−CPS (<b>top</b>) and CRA (<b>middle</b>) from 2001 to 2021, and their differences (<b>bottom</b>). (<b>a</b>,<b>e</b>,<b>i</b>) Summer, (<b>b</b>,<b>f</b>,<b>j</b>) June, (<b>c</b>,<b>g</b>,<b>k</b>) July, (<b>d</b>,<b>h</b>,<b>l</b>) August. The dotted areas represent regions with a relative variability of 0–10%.</p>
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<p>Spatial distribution of the differences in ground-air temperature (<b>top</b>), surface wind speed (<b>middle</b>), and specific humidity (<b>bottom</b>) on the TP as predicted by CMA−CPS and reanalyzed by CRA for different periods from 2001 to 2021 (units: °C, m/s, kg/kg, respectively). (<b>a</b>,<b>e</b>,<b>i</b>) Summer, (<b>b</b>,<b>f</b>,<b>j</b>) June, (<b>c</b>,<b>g</b>,<b>k</b>) July, (<b>d</b>,<b>h</b>,<b>l</b>) August.</p>
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21 pages, 8277 KiB  
Article
Identification and Expression Analysis of TCP Transcription Factors Under Abiotic Stress in Phoebe bournei
by Wenzhuo Lv, Hao Yang, Qiumian Zheng, Wenhai Liao, Li Chen, Yiran Lian, Qinmin Lin, Shuhao Huo, Obaid Ur Rehman, Wei Liu, Kehui Zheng, Yanzi Zhang and Shijiang Cao
Plants 2024, 13(21), 3095; https://doi.org/10.3390/plants13213095 - 3 Nov 2024
Viewed by 625
Abstract
The TCP gene family encodes plant transcription factors crucial for regulating growth and development. While TCP genes have been identified in various species, they have not been studied in Phoebe bournei (Hemsl.). This study identified 29 TCP genes in the P. bournei genome, [...] Read more.
The TCP gene family encodes plant transcription factors crucial for regulating growth and development. While TCP genes have been identified in various species, they have not been studied in Phoebe bournei (Hemsl.). This study identified 29 TCP genes in the P. bournei genome, categorizing them into Class I (PCF) and Class II (CYC/TB1 and CIN). We conducted analyses on the PbTCP gene at both the protein level (physicochemical properties) and the gene sequence level (subcellular localization, chromosomal distribution, phylogenetic relationships, conserved motifs, and gene structure). Most P. bournei TCP genes are localized in the nucleus, except PbTCP9 in the mitochondria and PbTCP8 in both the chloroplast and nucleus. Chromosomal mapping showed 29 TCP genes unevenly distributed across 10 chromosomes, except chromosome 8 and 9. We also analyzed the promoter cis-regulatory elements, which are mainly involved in plant growth and development and hormone responses. Notably, most PbTCP transcription factors respond highly to light. Further analysis revealed three subfamily genes expressed in five P. bournei tissues: leaves, root bark, root xylem, stem xylem, and stem bark, with predominant PCF genes. Using qRT-PCR, we examined six representative genes—PbTCP16, PbTCP23, PbTCP7, PbTCP29, PbTCP14, and PbTCP15—under stress conditions such as high temperature, drought, light exposure, and dark. PbTCP14 and PbTCP15 showed significantly higher expression under heat, drought, light and dark stress. We hypothesize that TCP transcription factors play a key role in growth under varying light conditions, possibly mediated by auxin hormones. This work provides insights into the TCP gene family’s functional characteristics and stress resistance regulation in P. bournei. Full article
(This article belongs to the Special Issue Molecular Biology and Bioinformatics of Forest Trees)
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<p>Conceptual framework of regulation of auxin signaling by <span class="html-italic">TCP</span> transcription factors. Note: The role of <span class="html-italic">TCP</span> transcription factors in regulating auxin biosynthesis and plant responses to abiotic stress. <span class="html-italic">TCP5</span>, <span class="html-italic">TCP13</span>, and <span class="html-italic">TCP17</span> enhance auxin synthesis by upregulating PIF, which in turn increases the expression of YUC enzymes, key players in the auxin biosynthesis pathway. Elevated auxin levels contribute to plant growth and stress adaptation. <span class="html-italic">TCP14</span> and <span class="html-italic">TCP15</span> specifically promote plant elongation by regulating auxin-induced genes associated with cell expansion. Together, these <span class="html-italic">TCP</span> factors support plant resilience under various abiotic stresses.</p>
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<p>Chromosomal localization analysis of the <span class="html-italic">TCP</span> gene family of <span class="html-italic">Phoebe bournei</span> (Hemsl.). Distribution of <span class="html-italic">PbTCP</span> genes in the <span class="html-italic">P. bournei</span> chromosome. (<b>A</b>) Each chromosome figure shows the chromosome number at the top. The scale on the left can be used to assess chromosome length and gene position. (<b>B</b>) The number of <span class="html-italic">TCP</span> genes on the chromosome.</p>
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<p>Phylogenetic analysis of <span class="html-italic">TCP</span> protein. (<b>A</b>) Genome and protein sequences <span class="html-italic">P. bournei</span>. (<b>B</b>) The percentage of 3 subfamilies of PbTCP genes. (<b>C</b>) Phylogenetic tree of <span class="html-italic">PbTCP</span> and <span class="html-italic">AtTCP</span> proteins. The arcs of different colors indicate a subfamily of the TCP family. One thousand times with MEGA11 and Bootstrap respectively. The tree was constructed by 29 <span class="html-italic">PbTCPs</span> identified in <span class="html-italic">P. bournei</span> and 25 <span class="html-italic">AtTCPs</span> identified in <span class="html-italic">A. thaliana</span>.</p>
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<p>Multiple sequence alignment of TCP domains. Note: TCP domain serial alignment of <span class="html-italic">P. bournei</span> TCP family members. At the bottom, the highly conserved amino acid position is indicated by the length of the rectangle; The serial indicator is displayed at the bottom.</p>
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<p><span class="html-italic">PbTCP</span> protein structure analysis. Note: The categories of three branches are marked on the left, and the confidence level of the protein’s secondary structure is indicated by different colors, and the four levels of confidence are shown in the lower right corner.</p>
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<p><span class="html-italic">PbTCP</span> conserved domain and motif analysis. (<b>A</b>) Phylogenetic tree of <span class="html-italic">PbTCPS</span>. (<b>B</b>) The motif of <span class="html-italic">PbTCPS</span>. Patterns 1–10 are displayed in rectangles of different colors. Protein length can be estimated using the scale at the bottom. (<b>C</b>) <span class="html-italic">PbTCP</span> protein with conserved domains. (<b>D</b>) Gene structure of the <span class="html-italic">PbTCPS</span> gene. Yellow boxes indicate exons (CDS), black lines indicate introns, and blue boxes indicate 5′ and 3′ untranslated regions. (<b>E</b>) The sequence logo of Motif1.The colored letters indicate the specific sequence of motif1.</p>
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<p>Genomic location, replication events, and homology of the <span class="html-italic">PbTCP</span> gene. Note: Synteny analysis of the <span class="html-italic">PbTCP</span> family in <span class="html-italic">P. bournei</span>. The gray line represents all isotope blocks in the <span class="html-italic">P. bournei</span> genome, while the red line represents the gene pairs of the duplicate <span class="html-italic">PbTCP</span>. The chromosome number is displayed in a rectangular box for each chromosome.</p>
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<p>Orthologous analysis of TCP genes in <span class="html-italic">A. thaliana</span>, <span class="html-italic">O. sativa</span>, <span class="html-italic">P. trichocarpa</span> and <span class="html-italic">P. bournei.</span> (<b>A</b>) Genome homology analysis of <span class="html-italic">A. thaliana</span> and <span class="html-italic">P. trichocarpus</span>. The grey line represents the genome pairs between homologous blocks, and the blue line highlights the <span class="html-italic">TCPS</span> gene pairs synthesized in the three species. (<b>B</b>) Number of genome pairs of three clades of different species. (<b>C</b>) Number of shared genes of three genome pairs of three species.</p>
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<p>Analysis of the cis-acting element of the gene for the promoter. (<b>A</b>) Cis-component predictions of 29 <span class="html-italic">PbTCP</span> gene promoter serial (−2000 bp) were analyzed using PlantCARE technology. Here are the 19 categories of cis-elements. (<b>B</b>) Number of 19 cis-components for the 29 <span class="html-italic">PbTCP</span> genes.</p>
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<p>Expression spectrum of <span class="html-italic">PbTCP</span>. Note: Different colors are used to indicate the level of expression, and there is an expression value on the right. At the bottom, there are three sub-categories with gene names.</p>
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<p>The expression of <span class="html-italic">PbTCPs</span> under high temperature, drought, light stress and dark stress was detected by qRT-PCR. (<b>A</b>) Relative gene expression levels at high temperature (40 °C) and control (25 °C). (<b>B</b>) Relative gene expression levels at the same point (4, 8, 12, and 24 h) were treated with 10% PEG nutrient solution in a simulated arid environment. The control group is treated in distilled water. (<b>C</b>) Relative gene expression levels under light stress. (<b>D</b>) Relative gene expression levels under dark stress. (* <span class="html-italic">p</span> &lt; 0.05, ** <span class="html-italic">p</span> &lt; 0.01, *** <span class="html-italic">p</span> &lt; 0.0005, **** <span class="html-italic">p</span> &lt; 0.0001).</p>
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<p>The different responses of PIFs and HY5 under Pfr and Pr form. Note: Light promotes the degradation of PIF and the expression of HY5 in the nucleus through photosensitive pigments (Pfr); Under dark conditions, the Pr form promotes the transcription and accumulation of PIFs and the degradation of HY5, which together regulate the normal physiological state of plants.</p>
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34 pages, 15689 KiB  
Article
Analysis of the Heat Transfer Performance of a Buried Pipe in the Heating Season Based on Field Testing
by Yongjie Ma, Jingyong Wang, Fuhang Hu, Echuan Yan, Yu Zhang, Yibin Huang, Hao Deng, Xuefeng Gao, Jianguo Kang, Haoxin Shi, Xin Zhang, Jianqiao Zheng and Jixiang Guo
Energies 2024, 17(21), 5466; https://doi.org/10.3390/en17215466 - 31 Oct 2024
Viewed by 439
Abstract
Ground source heat pump (GSHP) systems have been widely used in the field of shallow geothermal heating and cooling because of their high thermal efficiency and environmental friendliness. A borehole heat exchanger (BHE) is the key part of a ground source heat pump [...] Read more.
Ground source heat pump (GSHP) systems have been widely used in the field of shallow geothermal heating and cooling because of their high thermal efficiency and environmental friendliness. A borehole heat exchanger (BHE) is the key part of a ground source heat pump system, and its performance and investment cost have a direct and significant impact on the performance and cost of the whole system. The ground temperature gradient, air temperature, seepage flow rate, and injection flow rate affect the heat exchange performance of BHEs, but most of the research on BHEs lacks field test verification. Therefore, this study relied on the results of a field thermal response test (TRT) based on a distributed optical fiber temperature sensor (DOFTS) and site hydrological, geological, and geothermal data to establish a corrected numerical model of buried pipe heat transfer and carry out the heat transfer performance analysis of a buried pipe in the heating season. The results showed that the ground temperature gradient of the test site was about 3.0 °C/100 m, and the temperature of the constant-temperature layer was about 9.17 °C. Increasing the air temperature could improve the heat transfer performance. The temperature of the surrounding rock and soil mass of the single pipe spread uniformly, and the closer it was to the buried pipe, the lower the temperature. When there is groundwater seepage, the seepage carries the cold energy generated by a buried pipe’s heat transfer through heat convection to form a plume zone, which can effectively alleviate the phenomenon of cold accumulation. With an increase in seepage velocity, the heat transfer of the buried pipe increases nonlinearly. The heat transfer performance can be improved by appropriately reducing the temperature and velocity of the injected fluid. Selecting a backfill material with higher thermal conductivity than the ground body can improve the heat transfer performance. These research results can provide support for the optimization of the heat transfer performance of a buried tube heat exchanger. Full article
(This article belongs to the Section H2: Geothermal)
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<p>Geographical location of the study area and heating objectives.</p>
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<p>Temperature changes in the heating season of the study district.</p>
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<p>Combined thermal response test system. (<b>a</b>) Schematic diagram of the system. (<b>b</b>) Fixing optical fibers with ribbon. (<b>c</b>) High-temperature- and abrasion-resistant hose. (<b>d</b>) Optical fiber disassembly diagram. (<b>e</b>) TRT module. (<b>f</b>) DTRT module.</p>
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<p>Schematic diagram of simplified assumptions for the heat transfer module and model. (<b>a</b>) Heat transfer in porous media. (<b>b</b>) Non-isothermal pipeline flow.</p>
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<p>Vertical buried pipe heat transfer model. (<b>a</b>) Top view of the heat transfer model. (<b>b</b>) Grid encryption. (<b>c</b>) Model boundary.</p>
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<p>Initial formation temperature distribution with unheated and uncirculated fluid.</p>
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<p>Time evolution curves of inlet and outlet temperatures under heat load conditions: (<b>a</b>) 12 kW; (<b>b</b>) 8 kW.</p>
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<p>Field test of the layered thermal conductivity of the rock and soil mass.</p>
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<p>Grid independence test of the buried pipe heat transfer model.</p>
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<p>Comparison of the numerical results of heat transfer in the buried pipe and the results of the thermal response test in the field.</p>
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<p>Evolutionary characteristics of the heat exchanger outlet fluid temperature with time under three temperature conditions.</p>
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<p>Heat transfer capacities under different temperature conditions: (<b>a</b>) 108 days; (<b>b</b>) 174 days.</p>
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<p>Temperature evolution of the outlet fluid of the buried pipe heat exchanger with or without seepage.</p>
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<p>Heat transfer capacities of buried pipe heat exchangers with and without seepage conditions.</p>
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<p>Temperature evolution of the vertical cross-section of the buried tube heat exchanger without seepage.</p>
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<p>Temperature evolution of the vertical cross-section of the buried tube heat exchanger under seepage conditions.</p>
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<p>Temperature evolution of the middle section (z = 86 m) of the muddy sandstone layer without seepage.</p>
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<p>Temperature evolution of the middle section (z = 86 m) of the mudstone sandstone layer under seepage conditions.</p>
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<p>The influence of seepage velocity on the outlet fluid temperatures of heat exchangers.</p>
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<p>Heat transfer capacities of buried pipe heat exchangers with different seepage velocities.</p>
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<p>Temperature distributions in the mid-depth section (z = 86 m) of muddy siltstone layers with different seepage velocities.</p>
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<p>Influence of the inlet fluid temperature on the outlet fluid temperature of a heat exchanger.</p>
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<p>Heat transfer capacities of buried pipe heat exchangers with different inlet fluid temperatures.</p>
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<p>Temperature distribution in the middle depth section (z = 86 m) of muddy siltstone layers with different inlet fluid temperatures.</p>
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<p>The influence of fluid injection velocity on the outlet fluid temperature of heat exchangers.</p>
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<p>Heat transfer capacities of buried pipe heat exchangers with different injection flow rates.</p>
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<p>Temperature distribution at the middle depth section (z = 86 m) of muddy siltstone layers with different injection flow rates.</p>
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<p>The effects of backfill materials on the outlet temperature of heat exchanger fluid.</p>
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<p>Heat exchange capacities of buried pipe heat exchangers with different backfill materials (A: Bentonite; B: Fine sand, bentonite; C: Waste materials of silica; D: Aluminum shavings, cement, fine sand).</p>
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<p>Temperature distributions in the mid-depth section (z = 86 m) of different backfill materials in the muddy siltstone layer (A: Bentonite; B: Fine sand, bentonite; C: Waste materials of silica; D: Aluminum shavings, cement, fine sand).</p>
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19 pages, 4786 KiB  
Article
Dynamic Coupling Between Atmospheric CO2 Concentration and Land Surface Temperature in Major Urban Agglomerations in China: Insights for Sustainable Urban Development
by Qiwen Sun, Xuesheng Zhao and Yiying Hua
Sustainability 2024, 16(21), 9484; https://doi.org/10.3390/su16219484 - 31 Oct 2024
Viewed by 498
Abstract
To provide new insights into the integrated management of carbon and heat for sustainable urban development, this study systematically investigates the complex relationship between atmospheric CO2 concentrations and land surface temperature (LST). Utilizing OCO-2 and OCO-3 satellite observations, combined with meteorological conditions, [...] Read more.
To provide new insights into the integrated management of carbon and heat for sustainable urban development, this study systematically investigates the complex relationship between atmospheric CO2 concentrations and land surface temperature (LST). Utilizing OCO-2 and OCO-3 satellite observations, combined with meteorological conditions, air pollutants, and spatial characteristics, a high-resolution (0.1° × 0.1°) monthly CO2 column concentration (XCO2) dataset for China spanning 2015 to 2022 was generated using the Random Forest algorithm. The study focuses on urban agglomerations, conducting centroid migration and coupling analyses of XCO2 and LST to elucidate their spatiotemporal distribution patterns and evolution. Results reveal significant seasonal variations in XCO2, which has exhibited a gradual increase over the years. The spatiotemporal distributions of XCO2 and LST in urban agglomerations show a high degree of consistency, with centroids either converging or following similar movement trajectories. Additionally, the degree of coupling and coordination between XCO2 and LST has improved annually, indicating a closer interrelationship. These findings enhance our understanding of climate system dynamics and provide essential scientific evidence and decision-making support for addressing climate change. By clarifying the connection between atmospheric CO2 and LST, this study contributes to the development of more effective strategies for carbon reduction and urban heat island mitigation, thereby advancing cities towards greener, lower-carbon, and more sustainable development pathways. Full article
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<p>Geographical location of China’s five major urban agglomerations.</p>
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<p>Flow chart of this study.</p>
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<p>The locations of ground-based stations and the buffer zones with a radius of 1.5°.</p>
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<p>Monthly XCO<sub>2</sub> with 0. 1° × 0.1°spatial distribution from 2015 to 2022 in China.</p>
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<p>The fitting results of the Random Forest model.</p>
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<p>Ground validation results at TCCON stations.</p>
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<p>Comparisons of RF-XCO<sub>2</sub> with TCCON stations.</p>
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<p>The geographical locations of urban agglomerations and their XCO<sub>2</sub> and LST.</p>
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<p>The centroids of XCO<sub>2</sub> and LST from 2015 to 2022 in urban agglomerations.</p>
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<p>Spatial distribution of D values of urban agglomerations.</p>
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<p>The transfer trend of coordination levels after integration.</p>
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27 pages, 21466 KiB  
Article
Identifying Calmodulin and Calmodulin-like Protein Members in Canavalia rosea and Exploring Their Potential Roles in Abiotic Stress Tolerance
by Qianqian Ding, Zengwang Huang, Zhengfeng Wang, Shuguang Jian and Mei Zhang
Int. J. Mol. Sci. 2024, 25(21), 11725; https://doi.org/10.3390/ijms252111725 - 31 Oct 2024
Viewed by 589
Abstract
Calmodulins (CaMs) and calmodulin-like proteins (CMLs) belong to families of calcium-sensors that act as calcium ion (Ca2+) signal-decoding proteins and regulate downstream target proteins. As a tropical halophyte, Canavalia rosea shows great resistance to multiple abiotic stresses, including high salinity/alkalinity, extreme [...] Read more.
Calmodulins (CaMs) and calmodulin-like proteins (CMLs) belong to families of calcium-sensors that act as calcium ion (Ca2+) signal-decoding proteins and regulate downstream target proteins. As a tropical halophyte, Canavalia rosea shows great resistance to multiple abiotic stresses, including high salinity/alkalinity, extreme drought, heat, and intense sunlight. However, investigations of calcium ion signal transduction involved in the stress responses of C. rosea are limited. The CaM and CML gene families have been identified and characterized in many other plant species. Nevertheless, there is limited available information about these genes in C. rosea. In this study, a bioinformatic analysis, including the gene structures, conserved protein domains, phylogenetic relationships, chromosome distribution, and gene synteny, was comprehensively performed to identify and characterize CrCaMs and CrCMLs. A spatio-temporal expression assay in different organs and environmental conditions was then conducted using the RNA sequencing technique. Additionally, several CrCaM and CrCML members were then cloned and functionally characterized using the yeast heterogeneous expression system, and some of them were found to change the tolerance of yeast to heat, salt, alkalinity, and high osmotic stresses. The results of this study provide a foundation for understanding the possible roles of the CrCaM and CrCML genes, especially for halophyte C. rosea’s natural ecological adaptability for its native habitats. This study also provides a theoretical basis for further study of the physiological and biochemical functions of plant CaMs and CMLs that are involved in tolerance to multiple abiotic stresses. Full article
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<p>Locations of the 51 <span class="html-italic">CrCaM</span>s/<span class="html-italic">CrCML</span>s on 11 chromosomes of <span class="html-italic">Canavalia rosea</span>. Seven <span class="html-italic">CrCaM</span> genes were labeled with *.</p>
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<p>The distribution of segmental duplication of <span class="html-italic">CrCaM</span>s/<span class="html-italic">CrCML</span>s in <span class="html-italic">Canavalia rosea</span> chromosomes. Two <span class="html-italic">CrCaM</span> gene pairs (<span class="html-italic">CrCaM3</span> and <span class="html-italic">CrCaM4</span>, <span class="html-italic">CrCaM5</span> and <span class="html-italic">CrCaM7</span>) were marked with yellow background and red font.</p>
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<p>Exon–intron structures of the <span class="html-italic">CrCaM</span>s/<span class="html-italic">CrCML</span>s predicted using the Gene Structure Display Server (GSDS, <a href="http://gsds.cbi.pku.edu.cn/" target="_blank">http://gsds.cbi.pku.edu.cn/</a>, accessed on 10 April 2024).</p>
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<p>Structural analysis of the CrCaM/CrCMLs. The conserved motifs of each group identified using the MEME web server (<a href="http://meme-suite.org/index.html" target="_blank">http://meme-suite.org/index.html</a>, accessed on 10 April 2024). Different motifs are represented by different colored boxes, and the motif sequences are provided at the bottom.</p>
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<p>The phylogenetic tree of the CrCaMs/CrCMLs constructed using MEGA-X (version 10.1.8).</p>
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<p>Phylogenetic relationships of the 51 CrCaMs/CrCMLs from <span class="html-italic">Canavalia rosea</span>, 57 AtCaMs/AtCMLs from <span class="html-italic">Arabidopsis thaliana</span>, and 37 OsCaMs/OsCMLs from <span class="html-italic">Oryza sativa</span>. The amino acid sequences of these 145 CaMs/CMLs from the three plant species were compared with a ClustalW alignment, and the phylogenetic tree was constructed in MEGA-X using the neighbor-joining method with 1000 bootstrap repetitions. The different branch colors represent different subgroups.</p>
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<p>Statistics for the predicted <span class="html-italic">cis</span>-regulatory elements in the <span class="html-italic">CrCaM</span>s/<span class="html-italic">CrCML</span>s promoters (ATG upstream 2000 bp). (<b>A</b>) Summaries of the 13 <span class="html-italic">cis</span>-regulatory elements in the 51 <span class="html-italic">CrCaM</span>s/<span class="html-italic">CrCML</span>s promoter regions. (<b>B</b>) Distribution of these <span class="html-italic">cis</span>-regulatory elements in the 51 <span class="html-italic">CrCaM</span>s/<span class="html-italic">CrCML</span>s promoter regions. The elements are represented by different symbols. The scale bar represents 200 bp.</p>
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<p>Heatmaps showing (<b>A</b>) the expression levels of the <span class="html-italic">CrCaM</span>s/<span class="html-italic">CrCML</span>s in the root, vine, leaf, flower bud, and young fruit of <span class="html-italic">Canavalia rosea</span> plants and (<b>B</b>) the expression differences in the <span class="html-italic">CrCaM</span>s/<span class="html-italic">CrCML</span>s in mature <span class="html-italic">C. rosea</span> leaves planted in the South China National Botanical Garden (SCNBG) and in Yongxing (YX) Island.</p>
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<p>Heatmaps showing the expression levels of the RNA-Seq data for the <span class="html-italic">CrCaM</span>s/<span class="html-italic">CrCML</span>s in the root and leaf samples captured from the heat-shock-treated <span class="html-italic">Canavalia rosea</span> seedlings. The expression levels of each gene are shown in values of log2 (FPKM+1). Red denotes high expression levels and green denotes low expression levels.</p>
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<p>Heatmaps showing the expression changes in the RNA-Seq data for the <span class="html-italic">CrCaM</span>s/<span class="html-italic">CrCML</span>s under high salinity, alkaline, and high osmosis stresses. The expression levels of each gene are shown in values of log2 (FPKM+1). The expression differences of 51 <span class="html-italic">CrCaM</span>s/<span class="html-italic">CrCML</span>s in the root (<b>A</b>) and leaf (<b>B</b>) after the 2 h and 2 d abiotic stress challenges.</p>
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<p>Quantitative RT-PCR detection of the expression levels of the thirteen <span class="html-italic">CrCaM</span>s/<span class="html-italic">CrCML</span>s responding to different abiotic stresses, including heat (45 °C), high salt (600 mM NaCl), alkaline (150 mM NaHCO<sub>3</sub>, pH 8.2), high osmotic stress (300 mM mannitol), and H<sub>2</sub>O<sub>2</sub> (10 mM) oxidative stress. The relative expression values were calculated using the 2<sup>−ΔCt</sup> method with the housekeeping gene, <span class="html-italic">CrEF-1α</span>, as a reference gene. The bars show the mean values ± SDs of n = 3–4 technical replicates. Asterisks indicate significant differences from the CK (control check, without stress, Student’s <span class="html-italic">t</span>-test, * <span class="html-italic">p</span> &lt; 0.1, and ** <span class="html-italic">p</span> &lt; 0.01, *** <span class="html-italic">p</span> &lt; 0.001, and **** <span class="html-italic">p</span> &lt; 0.0001).</p>
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<p>The functional confirmation in yeast strain WTs and <span class="html-italic">skn7Δ</span> by expressing ten <span class="html-italic">CrCaM</span>s/<span class="html-italic">CrCML</span>s. Yeast cultures (WT: 52 °C for 30 min for heat stress; <span class="html-italic">skn7Δ</span>: 52 °C for 15 min for heat stress; or WT without challenges) were adjusted to OD600 = one, and 2 μL of serial dilutions (ten-fold) were spotted on SDG-Ura medium plates supplied with specific challenge stressors. The plates were incubated for 2–5 days at 30 °C. Functional identification of ten <span class="html-italic">CrCaM</span>s/<span class="html-italic">CrCML</span>s in yeast using a heterologous expression assay. (<b>A</b>) The thermotolerance confirmation in the yeast mutant strain, <span class="html-italic">skn7Δ</span>; (<b>B</b>) H<sub>2</sub>O<sub>2</sub> oxidative stress tolerance confirmation in the yeast mutant strain, <span class="html-italic">skn7Δ</span>; (<b>C</b>) high-salinity tolerance confirmation in the WT yeast on NaCl-surplus SDG-Ura medium plates; (<b>D</b>) high-alkaline tolerance confirmation in the WT yeast on NaHCO<sub>3</sub>-surplus SDG-Ura medium plates; and (<b>E</b>) the high-osmotic-stress tolerance confirmation in the WT yeast on sorbitol-surplus SDG-Ura medium plates.</p>
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