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13 pages, 1913 KiB  
Article
Green Veterinary Pharmacology Applied to Beekeeping: Semi-Field and Field Tests Against Varroa destructor, Using Essential Oil of Bergamot (Citrus bergamia) and Lemon (Citrus limon)
by Roberto Bava, Ernesto Palma, Rosa Maria Bulotta, Stefano Ruga, Giovanna Liguori, Renato Lombardi, Carmine Lupia, Mariangela Marrelli, Giancarlo Statti, Vincenzo Musella, Domenico Britti and Fabio Castagna
Vet. Sci. 2025, 12(3), 224; https://doi.org/10.3390/vetsci12030224 (registering DOI) - 2 Mar 2025
Abstract
Varroa destructor is an ectoparasitic mite that causes a true syndrome with complex symptoms in honeybees. For mite control, several synthetic active principles are commercially available. However, these formulations are proving less effective due to the acquisition of resistance mechanisms by the parasite. [...] Read more.
Varroa destructor is an ectoparasitic mite that causes a true syndrome with complex symptoms in honeybees. For mite control, several synthetic active principles are commercially available. However, these formulations are proving less effective due to the acquisition of resistance mechanisms by the parasite. Naturally derived compounds, such as essential oils, are less prone to generating resistance mechanisms in mite populations and have been proven effective in numerous independent laboratory investigations. In this study, semi-field and field tests were conducted to evaluate the acaricidal efficacy of two essential oils (Citrus bergamia and Citrus limon), which, in our previous residual contact toxicity tests, showed efficacy against Varroa mites. For semi-field cage tests, two-level cages were set up. Filter paper soaked with different concentrations of essential oils was placed in the lower compartment of the cage; 20 honeybees and 10 Varroa mites were allocated in the upper level. Mite detachment from the honeybees was evaluated after 24 and 48 h. For field tests, cardboard strips soaked with solutions of essential oils at different concentrations were inserted in the experimental hives. Weekly, the strips were replaced, and the fall of parasites on the diagnostic bottom board was assessed. Semi-field tests showed that the essential oils, at the concentrations used, were not toxic to bees. In the semi-field tests, an average acaricidal efficacy of 33% and 60% was obtained, at the highest concentration used, for bergamot and lemon essential oils, respectively. During the 4 weeks of exposure to 600 mL of BEO, the average mite mortality was 28.5%. In the experimental group treated with the intermediate concentration of 1200 mL, the average mite mortality at the end of four weeks was 30.2%, while with the highest concentration of 1800 mL, it was 40.3%. During the four weeks of exposure to 600 mL of LEO, the average mite mortality was 34.1%. In the experimental group treated with the 1200 mL concentration, the average mite mortality at the end of the four weeks was 46.6%, while with the highest concentration, it was 50.7%. This study shows that the acaricidal efficacy observed in field tests differs from that in laboratory tests. Innovative formulations are needed that allow the time-controlled and gradual release of essential oil in the field. Full article
(This article belongs to the Section Veterinary Physiology, Pharmacology, and Toxicology)
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Figure 1

Figure 1
<p>Effects of BEO on neutralization of mites. * <span class="html-italic">p</span> &lt; 0.05 vs. BEO (10 mg/mL) after 24 h; # <span class="html-italic">p</span> &lt; 0.05 vs. BEO (10 mg/mL) after 48 h; ^ <span class="html-italic">p</span> &lt; 0.05 vs. BEO (30 mg/mL) after 24 h.</p>
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<p>Effects of LEO on neutralization of mites. * <span class="html-italic">p</span> &lt; 0.05 vs. LEO (10 mg/mL) after 24 h; # <span class="html-italic">p</span> &lt; 0.05 vs. LEO (10 mg/mL) after 48 h; ^ <span class="html-italic">p</span> &lt; 0.05 vs. LEO (20 mg/mL) after 24 h; ° <span class="html-italic">p</span> &lt; 0.05 vs. LEO (20 mg/mL) after 48 h; <span>$</span> <span class="html-italic">p</span> &lt; 0.05 vs. LEO (30 mg/mL) after 24 h.</p>
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<p>Comparison of effectiveness between BEO and LEO. * <span class="html-italic">p</span> &lt; 0.05.</p>
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<p>(<b>A</b>) BEO and (<b>B</b>) LEO mortality rate; (<b>C</b>) mortality comparison for BEO and LEO essential oils.</p>
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<p>Comparison of effectiveness between BEO and LEO.</p>
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24 pages, 1902 KiB  
Article
Waveguide Quantum Electrodynamics: Tryptophans Entangled with Water as Data Qubits in a Microtubule
by Akihiro Nishiyama, Shigenori Tanaka and Jack Adam Tuszynski
Dynamics 2025, 5(1), 7; https://doi.org/10.3390/dynamics5010007 (registering DOI) - 1 Mar 2025
Abstract
In this paper, we introduce waveguide Quantum Electrodynamics (wQED) for the description of tryptophans in microtubules representing data qubits for information storage and, possibly, information processing. We propose a Hamiltonian in wQED and derive Heisenberg equations for qubits and photons. Using the Heisenberg [...] Read more.
In this paper, we introduce waveguide Quantum Electrodynamics (wQED) for the description of tryptophans in microtubules representing data qubits for information storage and, possibly, information processing. We propose a Hamiltonian in wQED and derive Heisenberg equations for qubits and photons. Using the Heisenberg equations, we derive time-evolution equations for the probability of qubits and the distribution of photons both at zero and finite temperature. We then demonstrate the resultant sub-radiance with small decay rates, which is required to achieve robust data qubits for information storage by coupling tryptophan residues containing data qubits with water molecules as Josephson quantum filters (JQFs). We also describe an oscillation processes of qubits in a tubulin dimer through the propagation of excitations with changing decay rates of JQFs. Data qubits are found to retain initial values by adopting sub-radiant states involving entanglement with water degrees of freedom. Full article
23 pages, 1927 KiB  
Article
A Contrast-Enhanced Approach for Aerial Moving Target Detection Based on Distributed Satellites
by Yu Li, Hansheng Su, Jinming Chen, Weiwei Wang, Yingbin Wang, Chongdi Duan and Anhong Chen
Remote Sens. 2025, 17(5), 880; https://doi.org/10.3390/rs17050880 (registering DOI) - 1 Mar 2025
Abstract
This study proposes a novel technique for detecting aerial moving targets using multiple satellite radars. The approach enhances the image contrast of fused local three-dimensional (3D) profiles. Exploiting global navigation satellite system (GNSS) satellites as illuminators of opportunity (IOs) has brought remarkable innovations [...] Read more.
This study proposes a novel technique for detecting aerial moving targets using multiple satellite radars. The approach enhances the image contrast of fused local three-dimensional (3D) profiles. Exploiting global navigation satellite system (GNSS) satellites as illuminators of opportunity (IOs) has brought remarkable innovations to multistatic radar. However, target detection is restricted by radiation sources since IOs are often uncontrollable. To address this, we utilize satellite radars operating in an active self-transmitting and self-receiving mode for controllability. The main challenge of multiradar target detection lies in effectively fusing the target echoes from individual radars, as the target ranges and Doppler histories differ. To this end, two periods, namely the integration period and detection period, are precisely designed. In the integration period, we propose a range difference-based positive and negative second-order Keystone transform (SOKT) method to make range compensation accurate. This method compensates for the range difference rather than the target range. In the detection period, we develop two weighting functions, i.e., the Doppler frequency rate (DFR) variance function and smooth spatial filtering function, to extract prominent areas and make efficient detection, respectively. Finally, the results from simulation datasets confirm the effectiveness of our proposed technique. Full article
28 pages, 8440 KiB  
Article
Feasibility Study of Biodegradable Vegetable Peels as Sustainable Fluid Loss Additives in Water-Based Drilling Fluids
by Olajide Ibrahim Oladipo, Foad Faraji, Hossein Habibi, Mardin Abdalqadir, Jagar A. Ali and Perk Lin Chong
J 2025, 8(1), 10; https://doi.org/10.3390/j8010010 (registering DOI) - 1 Mar 2025
Abstract
Drilling fluids are vital in oil and gas well operations, ensuring borehole stability, cutting removal, and pressure control. However, fluid loss into formations during drilling can compromise formation integrity, alter permeability, and risk groundwater contamination. Water-based drilling fluids (WBDFs) are favored for their [...] Read more.
Drilling fluids are vital in oil and gas well operations, ensuring borehole stability, cutting removal, and pressure control. However, fluid loss into formations during drilling can compromise formation integrity, alter permeability, and risk groundwater contamination. Water-based drilling fluids (WBDFs) are favored for their environmental and cost-effective benefits but often require additives to address filtration and rheological limitations. This study explored the feasibility of using vegetable waste, including pumpkin peel (PP), courgette peel (CP), and butternut squash peel (BSP) in fine (75 μm) and very fine (10 μm) particle sizes as biodegradable WBDF additives. Waste vegetable peels were processed using ball milling and characterized via FTIR, TGA, and EDX. WBDFs, prepared per API SPEC 13A with 3 wt% of added additives, were tested for rheological and filtration properties. Results highlighted that very fine pumpkin peel powder (PP_10) was the most effective additive, reducing fluid loss and filter cake thickness by 43.5% and 50%, respectively. PP_10 WBDF maintained mud density, achieved a pH of 10.52 (preventing corrosion), and enhanced rheological properties, including a 50% rise in plastic viscosity and a 44.2% increase in gel strength. These findings demonstrate the remarkable potential of biodegradable vegetable peels as sustainable WBDF additives. Full article
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Figure 1

Figure 1
<p>Flowchart of procedural steps.</p>
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<p>Process flow diagram for the synthesis and characterization of biodegradable drilling fluid additives from vegetable peels.</p>
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<p>EDX analysis for (<b>a</b>) butternut squash peel, (<b>b</b>) courgette peel, (<b>c</b>) pumpkin peel, and FESEM analysis for (<b>d</b>) butternut squash peel, (<b>e</b>) courgette peel, and (<b>f</b>) pumpkin peel.</p>
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<p>FTIR analysis for (<b>a</b>) butternut squash peel, (<b>b</b>) courgette peel, and (<b>c</b>) pumpkin peel.</p>
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<p>TGA analysis for (<b>a</b>) butternut squash peel, (<b>b</b>) courgette peel, and (<b>c</b>) pumpkin peel.</p>
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<p>TGA analysis for (<b>a</b>) butternut squash peel, (<b>b</b>) courgette peel, and (<b>c</b>) pumpkin peel.</p>
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<p>Yield point, plastic viscosity, and apparent viscosity of base mud (BM) and biodegradable drilling fluids with vegetable peel additives at particle sizes (<b>a</b>) below 75 µm and (<b>b</b>) below 10 µm.</p>
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<p>Initial and final gel strength of BM and biodegradable drilling fluids with vegetable peel additives at particle sizes (<b>a</b>) below 75 µm and (<b>b</b>) below 10 µm.</p>
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<p>The relationship between shear strain rate and shear stress of (<b>a</b>) biodegradable pumpkin peel at 75 µm particle size, (<b>b</b>) biodegradable pumpkin peel at 10 µm particle size, (<b>c</b>) biodegradable butternut squash peel at 75 µm particle size, (<b>d</b>) biodegradable at butternut squash peel 10 µm at particle size, (<b>e</b>) biodegradable courgette peel at 75 µm particle size, and (<b>f</b>) biodegradable courgette peel at 10 µm particle size.</p>
Full article ">Figure 8 Cont.
<p>The relationship between shear strain rate and shear stress of (<b>a</b>) biodegradable pumpkin peel at 75 µm particle size, (<b>b</b>) biodegradable pumpkin peel at 10 µm particle size, (<b>c</b>) biodegradable butternut squash peel at 75 µm particle size, (<b>d</b>) biodegradable at butternut squash peel 10 µm at particle size, (<b>e</b>) biodegradable courgette peel at 75 µm particle size, and (<b>f</b>) biodegradable courgette peel at 10 µm particle size.</p>
Full article ">Figure 8 Cont.
<p>The relationship between shear strain rate and shear stress of (<b>a</b>) biodegradable pumpkin peel at 75 µm particle size, (<b>b</b>) biodegradable pumpkin peel at 10 µm particle size, (<b>c</b>) biodegradable butternut squash peel at 75 µm particle size, (<b>d</b>) biodegradable at butternut squash peel 10 µm at particle size, (<b>e</b>) biodegradable courgette peel at 75 µm particle size, and (<b>f</b>) biodegradable courgette peel at 10 µm particle size.</p>
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<p>(<b>a</b>) Viscosity and (<b>b</b>) shear stress as functions of shear rates of BM and biodegradable drilling fluids with vegetable peel additives at particle sizes below 75 µm and 10 µm.</p>
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<p>(<b>a</b>) Mud balance test, (<b>b</b>) pH analysis of BM, and biodegradable drilling fluids with vegetable peel additives at particle sizes below 75 µm and 10 µm.</p>
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<p>(<b>a</b>) Mud balance test, (<b>b</b>) pH analysis of BM, and biodegradable drilling fluids with vegetable peel additives at particle sizes below 75 µm and 10 µm.</p>
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<p>(<b>a</b>) Filtration rate over time, (<b>b</b>) filtration rate at 30 min of BM, and biodegradable drilling fluids with vegetable peel additives at particle sizes below 75 µm and 10 µm.</p>
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<p>Filter cakes thickness of BM and biodegradable drilling fluids with vegetable peel additives at particle sizes below 75 µm and 10 µm.</p>
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<p>Mechanism of filter cake formation on the borehole wall using BM and biodegradable drilling fluids with vegetable peel additives at particle sizes below 75 µm and 10 µm. The direction of the drilling mud during the operation is shown by the arrows.</p>
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19 pages, 1954 KiB  
Article
Visual-Inertial-Wheel Odometry with Slip Compensation and Dynamic Feature Elimination
by Niraj Reginald, Omar Al-Buraiki, Thanacha Choopojcharoen, Baris Fidan and Ehsan Hashemi
Sensors 2025, 25(5), 1537; https://doi.org/10.3390/s25051537 (registering DOI) - 1 Mar 2025
Abstract
Inertial navigation systems augmented with visual and wheel odometry measurements have emerged as a robust solution to address uncertainties in robot localization and odometry. This paper introduces a novel data-driven approach to compensate for wheel slippage in visual-inertial-wheel odometry (VIWO). The proposed method [...] Read more.
Inertial navigation systems augmented with visual and wheel odometry measurements have emerged as a robust solution to address uncertainties in robot localization and odometry. This paper introduces a novel data-driven approach to compensate for wheel slippage in visual-inertial-wheel odometry (VIWO). The proposed method leverages Gaussian process regression (GPR) with deep kernel design and long short-term memory (LSTM) layers to model and mitigate slippage-induced errors effectively. Furthermore, a feature confidence estimator is incorporated to address the impact of dynamic feature points on visual measurements, ensuring reliable data integration. By refining these measurements, the system utilizes a multi-state constraint Kalman filter (MSCKF) to achieve accurate state estimation and enhanced navigation performance. The effectiveness of the proposed approach is demonstrated through extensive simulations and experimental validations using real-world datasets. The results highlight the ability of the method to handle challenging terrains and dynamic environments by compensating for wheel slippage and mitigating the influence of dynamic objects. Compared to conventional VIWO systems, the integration of GPR and LSTM layers significantly improves localization accuracy and robustness. This work paves the way for deploying VIWO systems in diverse and unpredictable environments, contributing to advancements in autonomous navigation and multi-sensor fusion technologies. Full article
(This article belongs to the Section Physical Sensors)
17 pages, 6414 KiB  
Article
miR-204-5p Protects Nephrin from Enzymatic Degradation in Cultured Mouse Podocytes Treated with Nephrotoxic Serum
by George Haddad and Judith Blaine
Cells 2025, 14(5), 364; https://doi.org/10.3390/cells14050364 (registering DOI) - 1 Mar 2025
Abstract
Nephrin is an essential constituent of the slit diaphragm of the kidney filtering unit. Loss of nephrin expression leads to protein leakage into the urine, one of the hallmarks of kidney damage. Autoantibodies against nephrin have been reported in patients with minimal change [...] Read more.
Nephrin is an essential constituent of the slit diaphragm of the kidney filtering unit. Loss of nephrin expression leads to protein leakage into the urine, one of the hallmarks of kidney damage. Autoantibodies against nephrin have been reported in patients with minimal change disease and recurrent focal segmental glomerulosclerosis. Understanding the mechanism of nephrin loss may help improve or lead to the development of novel treatment strategies. In this study, we demonstrated the important function of miR-204-5p expression on the protection of nephrin from anti-nephrin antibodies present in nephrotoxic serum (NTS). In addition, we identified that aspartyl protease cathepsin D is one enzyme that may be involved in nephrin enzymatic degradation and that cathepsin D is a direct target of miR-204-5p gene regulation. The regulation of miR-204-5p expression was determined to be regulated by the long noncoding RNA Josd1-ps. In an NTS in vivo animal model, treatment with the pan aspartic protease inhibitor Pepstatin A ameliorated renal damage. Finally, we showed that the expression of miR-204-5p had a nephrin-protecting function in vitro. Developing a method of delivery of miR-204-5p specifically to podocytes in vivo may provide a novel method of nephroprotection against nephrin autoantibodies. Full article
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Figure 1

Figure 1
<p>NTS targets nephrin when miR-204-5p expression is reduced. Immortalized mouse podocytes were treated with the miRNA control or miR-204-5p mimic or inhibitory sequences or left untreated prior to the addition of 1:200 nephrotoxic serum (NTS). The cell lysates were analyzed by Western blot (<b>A</b>) and probed for the podocyte’s markers nephrin, neph1, and podocin. Actin was used as a loading control. The blots are quantified in (<b>B</b>–<b>D</b>). The miR-204-5p sequence expressions were analyzed by real-time PCR, and the data were normalized to the expression of miR-16-5p (<b>E</b>). The presence of anti-nephrin antibodies in NTS was analyzed by Western blot (<b>F</b>) under native and reduced and denatured conditions. The immunofluorescence images in (<b>G</b>) show mouse podocytes transfected with miR-204-5p mimic, inhibitor, or control sequences prior to NTS treatment. The images were obtained using a Stedycon Abberior (STED) (Göttingen, Germany) and Olympus 1X81(Tokyo, Japan) confocal microscope and 100X objective lens. Nephrin is stained in green, actin is stained in red and the nucleus is stained with DAPI (blue). The scale bar represents 10 μm. The experiments were repeated 4 times (<span class="html-italic">n</span> = 4), and <span class="html-italic">p</span> values &lt; 0.05 were considered significant, **** <span class="html-italic">p</span> &lt; 0.0001.</p>
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<p>Nephrin degradation is mediated by an aspartyl enzyme. Immortalized mouse podocytes were transfected with miR-204-5p mimic, inhibitor, or control sequences and treated with protease inhibitor cocktail before treatment with NTS. As shown by the Western blot (<b>A</b>) and quantitation (<b>B</b>), protease inhibition prevented the NTS-induced degradation of nephrin, even when miR-204 was inhibited. Mouse podocytes treated with the miR-204-5p inhibitory sequence and various protease inhibitors before NTS treatment showed that the aspartic acid protease inhibitor Pepstatin A rescued nephrin from degradation (<b>C</b>), and this was evident through Western blot quantitation (<b>D</b>). The experiments were repeated 4 times (<span class="html-italic">n</span> = 4). <span class="html-italic">p</span> values &lt; 0.05 were considered significant ** <span class="html-italic">p</span> &lt; 0.003, <span class="html-italic">**** p</span> &lt; 0.001.</p>
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<p>LAMP1 and cathepsin D are targets of miR-204-5p. The overexpression of the miR-204-5p mimic sequence reduced the expression of LAMP1 (<b>A</b>,<b>C</b>) but had no effect on nephrin (<b>A</b>,<b>B</b>) or cathepsin E (<b>A</b>,<b>D</b>), as determined by Western blot analysis. Using cathepsin ELISA, the overexpression of miR-204-5p decreased cathepsin D expression (<b>E</b>). The effect of cathepsin D’s enzymatic activity on nephrin is shown in (<b>F</b>) using SDS-PAGE gel and silver staining. Cathepsin D treatment results in nephrin fragments (arrows). The experiments were repeated 4 times (<span class="html-italic">n</span> = 4). * <span class="html-italic">p</span> &lt; 0.05, *** <span class="html-italic">p</span> = 0.0007.</p>
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<p>LncJosd1-ps regulates the expression of miR-204-5p. LncJosd1-ps sequence was cloned into a lentiviral vector (<b>A</b>) to overexpress lncJosd1-ps and an antisense oligo (ASO) was designed (<b>B</b>) to reduce lncJosd1-ps expression. (<b>C</b>) shows that the overexpression of lncJosd1-ps reduced miR-204-5p expression, whereas lncJosd1-ps inhibition increased miR-204-5p expression (<b>D</b>). The subcellular location of lncJosd1-ps was determined, and it shows nuclear as well as cytoplasmic expressions (<b>E</b>). The experiments were repeated 4 times (<span class="html-italic">n</span> = 4). <span class="html-italic">p</span> values &lt; 0.05 were considered significant ** <span class="html-italic">p</span> &lt; 0.003, *** <span class="html-italic">p</span> = 0.0002, **** <span class="html-italic">p</span> &lt; 0.0001.</p>
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<p>Validation of miR-204-5p targets by luciferase assay. The miR-204-5p binding sites within LAMP1 and cathepsin D 3′ UTR and within the lncJosd1-ps nucleotide sequence are shown in (<b>A</b>). miR-204-5p interaction with LAMP1 3′ UTR is validated by a luciferase assay using intact LAMP1 or mutated binding sites (<b>B</b>), cathepsin D (<b>C</b>), and lncJosd1-ps (<b>D</b>). The red letters indicate the complimentary nucleotides between miR-204-5p and the target genes sequences. The experiments were repeated 3 times (<span class="html-italic">n</span> = 3). * <span class="html-italic">p</span> value &lt; 0.05 was considered significant from NT group.</p>
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<p>The effects of NTS on kidney function in vivo. Male C57Bl/6 mice were injected with 100 μL of NTS via the tail vein and euthanized after 1 week. Kidneys were excised, and total RNA was extracted and analyzed by real-time PCR for the expression levels of miR-204-5p (<b>A</b>), lncJosd1 (<b>B</b>), KIM1 (<b>C</b>), NGAL (<b>D</b>), nephrin (<b>E</b>) and podocin (<b>F</b>). Albuminuria was significantly increased in mice treated with NTS compared to the control mice (<b>G</b>). Figure (<b>H</b>) shows glomerular damage induced by NTS (PAS stain, scale bar 50 µm). Seven to eight animals were included per group. * <span class="html-italic">p</span> &lt; 0.05, ** <span class="html-italic">p</span> &lt; 0.003, *** <span class="html-italic">p</span> = 0.0002, **** <span class="html-italic">p</span> &lt; 0.0001.</p>
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<p>Pepstatin A reduced kidney injury in the NTS model of immune-mediated kidney disease. Mouse kidney total RNA was analyzed for miR-204-5p and lncJosd1-ps expression in animals treated with NTS followed by IP injection of Pepstatin A (20 mg/Kg) or carrier (100 µL ethanol) on days 1, 3, and 5 (<b>A</b>,<b>B</b>). The expression levels of the kidney injury markers KIM1 (<b>C</b>) and NGAL (<b>D</b>) were determined at the mRNA level along with the podocyte markers nephrin (<b>E</b>) and podocin (<b>F</b>). Kidney morphology was assessed using PAS stain (<b>G</b>). Glomerulosclerosis was determined using a 0–4 scoring system, where 0 = 0%, 1 = 25%, 2 = 50%, 3 = 75%, and 4 = 100% glomerulosclerosis. All available cortical glomeruli in a PAS-stained tissue section were analyzed (<b>H</b>). Albuminuria was determined using the albumin-to-creatinine ratio (<b>I</b>). At least 5–8 animals were used per group. * <span class="html-italic">p</span> &lt; 0.05 different from control group, # different from NTS group <span class="html-italic">p</span> &lt; 0.002, NS = not significant from control; scale bars: 50 µm.</p>
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18 pages, 5532 KiB  
Article
Field Data Retrieval for Electric Vehicles and Estimating Equivalent Circuit Model Parameters via Particle Swarm Optimization
by Syed Adil Sardar, Shahzad Iqbal, Jeongju Park, Sekyung Han and Woo Young Kim
Technologies 2025, 13(3), 91; https://doi.org/10.3390/technologies13030091 (registering DOI) - 1 Mar 2025
Abstract
Data retrieval techniques are crucial for developing an effective battery management system for an electric vehicle to accurately assess the battery’s health and performance by monitoring operating conditions such as voltage, current, time, temperature, and state of charge. This paper proposes an efficient [...] Read more.
Data retrieval techniques are crucial for developing an effective battery management system for an electric vehicle to accurately assess the battery’s health and performance by monitoring operating conditions such as voltage, current, time, temperature, and state of charge. This paper proposes an efficient approach to retrieve real-world field data (voltage, current, and time) under running vehicle conditions. In the first step, noise is removed from the field data using a moving-average filter. Then, first- and second-order derivations are applied to the filtered data to determine specific data set conditions. After that, a new approach based on zero-crossing is implemented to retrieve the field data. A second-order Randle circuit (SORC) is utilized in this study to analyze the selected field data. Further, a particle swarm optimization algorithm is adapted to estimate the parameters of the SORC. Our experiments indicate that the relative errors of the equivalent circuit model (ECM) are less than 2% compared to the model voltage and real voltage, which is consistent with the stable parameters of ECM. Full article
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Graphical abstract

Graphical abstract
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<p>Fundamental concept of PSO.</p>
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<p>(<b>a</b>) ECM with FORC and (<b>b</b>) ECM with FORC and SORC.</p>
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<p>Comparison of the output from the FORC and SORC models.</p>
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<p>Battery tester setup (BT).</p>
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<p>(<b>a</b>) Field data; (<b>b</b>) field data with moving_avg function; (<b>c</b>) field data after noise is removed.</p>
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<p>Matlab code for the function of moving average filter.</p>
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<p>(<b>a</b>) shows the derivative of the current with respect to time, (<b>b</b>) represents the voltage field data as a function of time, with the red dots indicating the zero-crossing points, and (<b>c</b>) shows that the colored part is the selected field data.</p>
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<p>(<b>a</b>) Retrieved field data; (<b>b</b>) retrieved field data after filtering.</p>
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<p>Methodology for retrieving field data and estimating ECM parameters.</p>
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<p>PSO flow chart.</p>
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<p>Voltage with OCV impact.</p>
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<p>(<b>a</b>) Charge pulse; (<b>b</b>) discharge pulse.</p>
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<p>(<b>a</b>) Comparison of real data and simulation data without OCV impact for charge pulse. (<b>b</b>) Comparison of real data and simulation data without OCV impact for discharge pulse.</p>
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<p>(<b>a</b>) Comparison of real voltage and terminal voltage. (<b>b</b>) Comparison of filtered real voltage and filtered terminal voltage.</p>
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34 pages, 8962 KiB  
Review
Moisture Ingress in Building Envelope Materials: (I) Scientometric Analysis and Experimental Fundamentals
by Mohammad Hossein Yari, Elnaz Esmizadeh, Esrat Jahan, Itzel Lopez-Carreon, Marzieh Riahinezhad, Jacynthe Touchette, Zhe Xiao, Michael Lacasse and Elena Dragomirescu
Buildings 2025, 15(5), 798; https://doi.org/10.3390/buildings15050798 (registering DOI) - 1 Mar 2025
Abstract
Moisture ingress is a critical concern in buildings, as it may profoundly affect structural integrity, the energy efficiency of a building, and as well the quality of the indoor environment that, in turn, could influence the health and safety of building occupants. Moisture [...] Read more.
Moisture ingress is a critical concern in buildings, as it may profoundly affect structural integrity, the energy efficiency of a building, and as well the quality of the indoor environment that, in turn, could influence the health and safety of building occupants. Moisture ingress can occur during any phase in the lifecycle of a building component, where environmental loads, such as precipitation, wind, snow, and elevated relative humidity, play a fundamental role in affecting the building structure. Climate change exacerbates the issue of moisture ingress by intensifying these loads. In this review paper, the statistical perspective on publications related to moisture ingress in building envelope materials (BEMs) was first assessed through a scientometric study. All relevant publications were gathered and manually filtered, and the selected papers were categorized based on the topics discussed. The results of the scientometric study, as presented in this paper, include a bar chart in which the number of publications in each category is illustrated; a science journal mapping diagram showing the interdisciplinary connections of the research; a cluster map depicting the network between topics; and an R&D momentum analysis reflecting the rate of growth and publication count in this field. Given the strong focus on material properties, this review also examines experimental methods for characterizing moisture transport properties in building materials used in BEMs. Additionally, the differences between various codes and standards centered on this topic are reviewed and discussed. This combined strategy is intended to comprehensively evaluate available information and approaches to permit identifying the knowledge gaps that need to be addressed. Full article
(This article belongs to the Section Building Energy, Physics, Environment, and Systems)
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<p>Building envelope components typically used in North America for a cold climate, showing a basic multi-layer wall (<b>left</b>), and a retrofitted externally insulated wall (<b>right</b>).</p>
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<p>Distribution of articles related to moisture ingress in building envelopes across the ten key categories.</p>
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<p>Ten key categories reviewed in the context of moisture ingress in building envelopes, including relevant topics and the number of results.</p>
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<p>R&amp;D momentum of topics used in moisture ingress in building materials.</p>
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<p>Distribution of research on moisture ingress in building envelopes by world region.</p>
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<p>Distribution of research on building components related to moisture ingress in building envelopes.</p>
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<p>Distribution of research on building materials related to moisture ingress in building envelopes.</p>
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<p>Topic cluster map, showing 30% coefficient of correlation between the most frequent topics on moisture ingress in building envelopes.</p>
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<p>Distribution of scientific contributions by field to the study of moisture ingress in building materials (publications ≥ 30).</p>
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<p>Schematic diagram of capillary water absorption test.</p>
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<p>The evolution of water uptake (bright areas) in mortar (dark areas) as obtained by X-ray computed tomography (adapted from [<a href="#B58-buildings-15-00798" class="html-bibr">58</a>]).</p>
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<p>Diagram of a general setup for measurement of gamma-ray Compton scattering events.</p>
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<p>Single-sided NMR-MOUSE probe with a motorized lift for depth profiling.</p>
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<p>(<b>Left</b>) Experimental setup for determination of capillary water absorption coefficient and water diffusion coefficient by infrared thermography coupled with gravimetry (adapted from [<a href="#B76-buildings-15-00798" class="html-bibr">76</a>]) and (<b>Right</b>) Thermal images of gypsum acquired at times: (<b>a</b>) 10 s, (<b>b</b>) 900 s, (<b>c</b>) 1800 s, (<b>d</b>) 3600 s (reprinted with permission from [<a href="#B76-buildings-15-00798" class="html-bibr">76</a>]).</p>
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<p>Simplified schematic diagram of the semi-industrial convective air chamber (adapted from [<a href="#B78-buildings-15-00798" class="html-bibr">78</a>]).</p>
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<p>Schematic of equipment for total immersion test (adapted from [<a href="#B82-buildings-15-00798" class="html-bibr">82</a>]).</p>
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<p>Novel experimental setup for vapour diffusion measurements (adapted from [<a href="#B100-buildings-15-00798" class="html-bibr">100</a>]).</p>
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18 pages, 3530 KiB  
Article
PPRD-FL: Privacy-Preserving Federated Learning Based on Randomized Parameter Selection and Dynamic Local Differential Privacy
by Jianlong Feng, Rongxin Guo and Jianqing Zhu
Electronics 2025, 14(5), 990; https://doi.org/10.3390/electronics14050990 (registering DOI) - 28 Feb 2025
Abstract
As traditional federated learning algorithms often fall short in providing privacy protection, a growing body of research integrates local differential privacy methods into federated learning to strengthen privacy guarantees. However, under a fixed privacy budget, with the increase in the dimensionality of model [...] Read more.
As traditional federated learning algorithms often fall short in providing privacy protection, a growing body of research integrates local differential privacy methods into federated learning to strengthen privacy guarantees. However, under a fixed privacy budget, with the increase in the dimensionality of model parameters, the privacy budget allocated per parameter diminishes, which means that a larger amount of noise is required to meet privacy requirements. This escalation in noise may adversely affect the final model’s performance. For that, we propose a privacy protection federated learning (PPRD-FL) approach. First, we design a randomized parameter selection strategy that combines randomization with importance-based filtering, effectively addressing the privacy budget dilution problem by selecting only the most crucial parameters for global aggregation. Second, we develop a dynamic local differential privacy-based perturbation mechanism, which adjusts the noise levels according to the training phase, not only providing robustness and security but also optimizing the dynamic allocation of the privacy budget. Finally, our experiments have demonstrated that the proposed approach maintains a high performance while ensuring strong privacy guarantees. Full article
(This article belongs to the Special Issue Security and Privacy in Emerging Technologies)
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<p>Privacy risks in federated learning.</p>
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<p>Architecture of the proposed privacy-preserving federated learning approach based on randomized parameter selection and dynamic local differential privacy (PPRD-FL).</p>
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<p>Impact of R-PSS and DLDP on model accuracy and loss over global rounds: (<b>a</b>) accuracy and (<b>b</b>) loss.</p>
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<p>Model accuracy and loss under different privacy levels <math display="inline"><semantics> <mi>ε</mi> </semantics></math> on the MNIST dataset: (<b>a</b>) accuracy and (<b>b</b>) loss.</p>
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<p>Model accuracy and loss under different privacy levels <math display="inline"><semantics> <mi>ε</mi> </semantics></math> on the Fashion-MNIST dataset: (<b>a</b>) accuracy and (<b>b</b>) loss.</p>
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<p>Model accuracy and loss under different privacy levels <math display="inline"><semantics> <mi>ε</mi> </semantics></math> on the CIFAR-10 dataset: (<b>a</b>) accuracy and (<b>b</b>) loss.</p>
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<p>Model accuracy under different schemes.</p>
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24 pages, 12738 KiB  
Article
An Adaptive Weighted Residual-Guided Algorithm for Non-Uniformity Correction of High-Resolution Infrared Line-Scanning Images
by Mingsheng Huang, Weicong Chen, Yaohua Zhu, Qingwu Duan, Yanghang Zhu and Yong Zhang
Sensors 2025, 25(5), 1511; https://doi.org/10.3390/s25051511 (registering DOI) - 28 Feb 2025
Abstract
Gain and bias non-uniformities in infrared line-scanning detectors often result in horizontal streak noise, degrading image quality. This paper introduces a novel non-uniformity correction algorithm combining residual guidance and adaptive weighting, which achieves superior denoising and detail preservation compared to existing methods. The [...] Read more.
Gain and bias non-uniformities in infrared line-scanning detectors often result in horizontal streak noise, degrading image quality. This paper introduces a novel non-uniformity correction algorithm combining residual guidance and adaptive weighting, which achieves superior denoising and detail preservation compared to existing methods. The method combines residual and original images in a dual-guidance mechanism and significantly enhances denoising performance and detail preservation through iterative compensation strategies and locally weighted linear regression. Additionally, the algorithm employs local variance to adjust weights dynamically, achieving efficient correction in complex scenes while reducing computational complexity to meet real-time application requirements. Experimental results on both simulated and real infrared datasets demonstrate that the proposed method outperforms mainstream algorithms regarding peak signal-to-noise ratio (PSNR) and structural similarity (SSIM) metrics, achieving an optimal balance between detail preservation and noise suppression. The algorithm demonstrates robust performance in complex scenes, making it suitable for real-time applications in high-resolution infrared imaging systems. Full article
(This article belongs to the Section Electronic Sensors)
16 pages, 758 KiB  
Article
Antioxidant and Photoprotective Activity of Bromelain Cream: An In Vitro and In Vivo Study
by Zahra Marissa, Soraya Ratnawulan Mita, Cahya Khairani Kusumawulan and Sriwidodo Sriwidodo
Cosmetics 2025, 12(2), 41; https://doi.org/10.3390/cosmetics12020041 - 28 Feb 2025
Abstract
Bromelain, a natural enzyme derived from pineapple, is known for its antioxidant properties, and its potential as a photoprotective agent has garnered interest in skincare applications. The primary objective of this research was to evaluate and optimize the effectiveness of bromelain-based creams in [...] Read more.
Bromelain, a natural enzyme derived from pineapple, is known for its antioxidant properties, and its potential as a photoprotective agent has garnered interest in skincare applications. The primary objective of this research was to evaluate and optimize the effectiveness of bromelain-based creams in providing antioxidant and photoprotective protection against ultraviolet (UV) radiation. Antioxidant activity was assessed using the DPPH radical scavenging assay, and the Sun Protection Factor (SPF) was determined in vitro and in vivo to evaluate photoprotective activity. The results revealed that bromelain exhibited strong antioxidant activity. Photoprotection, as measured by SPF, the formulation F3, which combined bromelain with other UV filters, exhibited the highest SPF values of 22.043 ± 0.277 (in vitro) and 21.3 ± 2.901 (in vivo), indicating enhanced photoprotective efficacy. This improvement in SPF was likely due to the synergistic effect of bromelain with the UV filters Octyl Methoxycinnamate (OMC). The findings suggest a positive correlation between antioxidant activity and photoprotection, with bromelain’s antioxidant properties contributing to its overall photoprotective effect. Bromelain may be used on people without causing skin or eye irritation. This study supports the potential of bromelain-based creams as dual-action skincare formulations, offering both antioxidant and UV protection. Full article
18 pages, 309 KiB  
Review
Next-Generation Biomaterials for Vital Pulp Therapy: Exploring Biological Properties and Dentin Regeneration Mechanisms
by Vidhyashree Rajasekar, Mohamed Mahmoud Abdalla, Mengyu Huang, Prasanna Neelakantan and Cynthia Kar Yung Yiu
Bioengineering 2025, 12(3), 248; https://doi.org/10.3390/bioengineering12030248 - 28 Feb 2025
Abstract
The advancement of Vital Pulp Therapy (VPT) in dentistry has shown remarkable progress, with a focus on innovative materials and scaffolds to facilitate reparative dentin formation and tissue regeneration. A comprehensive search strategy was performed across PubMed, Scopus, and Web of Science using [...] Read more.
The advancement of Vital Pulp Therapy (VPT) in dentistry has shown remarkable progress, with a focus on innovative materials and scaffolds to facilitate reparative dentin formation and tissue regeneration. A comprehensive search strategy was performed across PubMed, Scopus, and Web of Science using keywords such as “vital pulp therapy”, “biomaterials”, “dentin regeneration”, and “growth factors”, with filters for English language studies published in the last 10 years. The inclusion criteria focused on in vitro, in vivo, and clinical studies evaluating traditional and next-generation biomaterials for pulp capping and tissue regeneration. Due to the limitations of calcium-based cements in tissue regeneration, next-generation biomaterials like gelatin, chitosan, alginate, platelet-rich fibrins (PRF), demineralized dentin matrix (DDM), self-assembling peptides, and DNA-based nanomaterials were explored for their enhanced biocompatibility, antibacterial properties, and regenerative potential. These biomaterials hold great potential in enhancing VPT outcomes, but further research is required to understand their efficacy and impact on dentin reparative properties. This review explores the mechanisms and properties of biomaterials in dentin tissue regeneration, emphasizing key features that enhance tissue regeneration. These features include biomaterial sources, physicochemical properties, and biological characteristics that support cells and functions. The discussion also covers the biomaterials’ capability to encapsulate growth factors for dentin repair. The development of innovative biomaterials and next-generation scaffold materials presents exciting opportunities for advancing VPT in dentistry, with the potential to improve clinical outcomes and promote tissue regeneration in a safe and effective manner. Full article
(This article belongs to the Special Issue Applications of Biomaterials in Dental Medicine)
30 pages, 4653 KiB  
Article
Commanded Filter-Based Robust Model Reference Adaptive Control for Quadrotor UAV with State Estimation Subject to Disturbances
by Nigar Ahmed and Nashmi Alrasheedi
Drones 2025, 9(3), 181; https://doi.org/10.3390/drones9030181 - 28 Feb 2025
Abstract
Unmanned aerial vehicles must achieve precise flight maneuvers despite disturbances, parametric uncertainties, modeling inaccuracies, and limitations in onboard sensor information. This paper presents a robust adaptive control for trajectory tracking under nonlinear disturbances. Firstly, parametric and modeling uncertainties are addressed using model reference [...] Read more.
Unmanned aerial vehicles must achieve precise flight maneuvers despite disturbances, parametric uncertainties, modeling inaccuracies, and limitations in onboard sensor information. This paper presents a robust adaptive control for trajectory tracking under nonlinear disturbances. Firstly, parametric and modeling uncertainties are addressed using model reference adaptive control principles to ensure that the dynamics of the aerial vehicle closely follow a reference model. To address the effects of disturbances, a modified nonlinear disturbance observer is designed based on estimated state variables. This observer effectively attenuates constant, nonlinear disturbances with variable frequency and magnitude, and noises. In the next step, a two-stage sliding mode control strategy is introduced, incorporating adaptive laws and a commanded-filter to compute numerical derivatives of the state variables required for control design. An error compensator is integrated into the framework to reduce numerical and computational delays. To address sensor inaccuracies and potential failures, a high-gain observer-based state estimation technique is employed, utilizing the separation principle to incorporate estimated state variables into the control design. Finally, Lyapunov-based stability analysis demonstrates that the system is uniformly ultimately bounded. Numerical simulations on a DJI F450 quadrotor validate the approach’s effectiveness in achieving robust trajectory tracking under disturbances. Full article
(This article belongs to the Section Drone Design and Development)
18 pages, 8374 KiB  
Article
Optimal Control Model of Electromagnetic Interference and Filter Design in Motor Drive System
by Shufen Wang, Wei Zhao, Xianming Zong and Wenzhuo Zhang
Electronics 2025, 14(5), 980; https://doi.org/10.3390/electronics14050980 - 28 Feb 2025
Abstract
Electromagnetic interference (EMI) is a key problem in the design of electric vehicle motor drive systems. Based on the system composition and conducted EMI mechanism, an equivalent circuit model including a motor, inverter, cable, and battery is established, and an optimized double closed-loop [...] Read more.
Electromagnetic interference (EMI) is a key problem in the design of electric vehicle motor drive systems. Based on the system composition and conducted EMI mechanism, an equivalent circuit model including a motor, inverter, cable, and battery is established, and an optimized double closed-loop control strategy is proposed. Through the joint simulation platform of Simulink and Simplorer, the conduction EMI prediction model of a motor drive system is constructed. On this basis, a filter design method based on Π-type topology is proposed based on the 6 dB safety margin of reducing the interference limit. The simulation results show that the designed filter significantly suppresses the conducted EMI in the frequency band from 150 kHz to 30 MHz, and the interference peak is reduced by approximately 40 dBμV. The effectiveness of the model and filter is verified by experimental tests, and the electromagnetic compatibility (EMC) performance of the system is improved, which provides theoretical support and an engineering reference for the high-frequency interference suppression of the motor drive system. Full article
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<p>Schematic diagram of the motor drive system.</p>
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<p>IGBT modeling process.</p>
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<p>The output characteristic curve and transfer characteristic curve in IGBT data sheet.</p>
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<p>IGBT static characteristic measurement circuit.</p>
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<p>Transfer characteristic curves (<b>a</b>) and output characteristic curves (<b>b</b>).</p>
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<p>π-type equivalent circuit model single-phase impedance (<b>a</b>) and three-phase impedance (<b>b</b>).</p>
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<p>Single-phase differential-mode impedance curve (<b>a</b>) and single-phase common-mode impedance curve (<b>b</b>).</p>
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<p>Single-phase differential-mode impedance curve (<b>a</b>) and single-phase common-mode impedance curve (<b>b</b>).</p>
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<p>Equivalent circuit diagram of the motor.</p>
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<p>Multi-conductor transmission line theory model (<b>a</b>) and simplified model (<b>b</b>).</p>
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<p>Cable cross-section diagram.</p>
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<p>Cable circuit model DC busbar (<b>a</b>) and AC busbar (<b>b</b>).</p>
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<p>Motor control model.</p>
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<p>SVPWM model.</p>
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<p>Motor speed (<b>a</b>), current curves (<b>b</b>), conduction EMI system model of the motor drive system (<b>c</b>) and EMI prediction waveform (<b>d</b>).</p>
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<p>Differential and common-mode interference. (<b>a</b>) Differential-mode interference. (<b>b</b>) Common-mode interference.</p>
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<p>Comparison of insertion loss between L-type and Π-type filters.</p>
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<p>Common-mode filter circuit diagram (<b>a</b>) and common-mode interference waveform (<b>b</b>).</p>
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<p>Differential-mode filter circuit diagram (<b>a</b>) and differential-mode interference waveform (<b>b</b>).</p>
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<p>Simulation and experimental results with installed filters.</p>
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20 pages, 11784 KiB  
Article
An Optimized Maximum Second-Order Cyclostationary Blind Deconvolution and Bidirectional Long Short-Term Memory Network Model for Rolling Bearing Fault Diagnosis
by Jixin Liu, Liwei Deng, Yue Cao, Chenglin Wen, Zhihuan Song, Mei Liu and Xiaowei Cui
Sensors 2025, 25(5), 1495; https://doi.org/10.3390/s25051495 - 28 Feb 2025
Abstract
To address the challenge of extracting fault features and accurately identifying bearing fault conditions under strong noisy environments, a rolling bearing failure diagnostic technique is presented that utilizes parameter-optimized maximum second-order cyclostationary blind deconvolution (CYCBD) and bidirectional long short-term memory (BiLSTM) networks. Initially, [...] Read more.
To address the challenge of extracting fault features and accurately identifying bearing fault conditions under strong noisy environments, a rolling bearing failure diagnostic technique is presented that utilizes parameter-optimized maximum second-order cyclostationary blind deconvolution (CYCBD) and bidirectional long short-term memory (BiLSTM) networks. Initially, an adaptive golden jackal optimization (GJO) algorithm is employed to refine important CYCBD parameters. Subsequently, the rolling bearing failure signals are filtered and denoised using the optimized CYCBD, producing a denoised signal. Ultimately, the noise-reduced signal is fed into the BiLSTM model to realize the classification of faults. The experimental findings demonstrate the suggested approach’s strong noise reduction performance and high diagnostic accuracy. The optimized CYCBD–BiLSTM improves the accuracy by approximately 9.89% compared with other methods when the signal-to-noise ratio (SNR) reaches −9 dB, and it can be effectively used for diagnosing rolling bearing faults under noisy backgrounds. Full article
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<p>GJO-CYCBD algorithm flowchart.</p>
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<p>LSTM architecture.</p>
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<p>BiLSTM network structure.</p>
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<p>The fault diagnosis process of the proposed method.</p>
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<p>Flowchart of rolling bearing fault diagnosis based on a CYCBD–BiLSTM approach.</p>
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<p>Simulated signal waveform and spectrum. (<b>a</b>) The time-domain waveform of the fault signal; (<b>b</b>) the time-domain waveform of the noise signal; (<b>c</b>) the time-domain waveform of a mixed-signal system; (<b>d</b>) the envelope spectrum of the mixed signal.</p>
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<p>The curve of Ec with population iteration.</p>
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<p>Envelope spectrum post-CYCBD processing. (<b>a</b>) The envelope spectrum after processing with optimal CYCBD parameters; (<b>b</b>) the envelope spectrum of the signal after modifying optimization parameters.</p>
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<p>CWRU testing rig.</p>
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<p>Comparison of diagnostic accuracy of BiLSTM at different learning rates.</p>
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<p>Comparison of fault diagnosis accuracy after different preprocessing.</p>
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<p>Experimental results under different noise levels.</p>
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<p>Performance of different models in diagnosis. (<b>a</b>) Best precision of the model; (<b>b</b>) best precision of the last epoch; (<b>c</b>) average precision of the last epoch.</p>
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<p>Confusion matrix of classification accuracy of the network model for CWRU data at SNR −6 dB. (<b>a</b>) Classification results of the last epoch of CYCBD-MLP; (<b>b</b>) classification results of the last epoch of CYCBD-CNN; (<b>c</b>) classification results of the last epoch of CYCBD–BiLSTM.</p>
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<p>Confusion matrix of classification accuracy of the network model for CWRU data at SNR −6 dB. (<b>a</b>) Classification results of the last epoch of CYCBD-MLP; (<b>b</b>) classification results of the last epoch of CYCBD-CNN; (<b>c</b>) classification results of the last epoch of CYCBD–BiLSTM.</p>
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